##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937698_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 552016 Sequences flagged as poor quality 0 Sequence length 101 %GC 42 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.785783383090344 33.0 31.0 34.0 30.0 34.0 2 31.87435146807339 33.0 31.0 34.0 30.0 34.0 3 31.899425016666182 33.0 31.0 34.0 30.0 34.0 4 35.5036194603055 37.0 35.0 37.0 33.0 37.0 5 35.49953986840961 37.0 35.0 37.0 33.0 37.0 6 35.61507093997275 37.0 35.0 37.0 33.0 37.0 7 35.566914727109356 37.0 35.0 37.0 33.0 37.0 8 35.565344120460274 37.0 35.0 37.0 33.0 37.0 9 37.162475363032954 39.0 37.0 39.0 34.0 39.0 10-11 37.0579847323266 39.0 37.0 39.0 33.0 39.0 12-13 36.92776839801745 39.0 37.0 39.0 33.0 39.0 14-15 37.98080852728907 40.0 38.0 41.0 33.0 41.0 16-17 37.94244913190922 40.0 38.0 41.0 33.0 41.0 18-19 37.996765492304576 40.0 38.0 41.0 33.0 41.0 20-21 37.86620496507348 40.0 38.0 41.0 33.0 41.0 22-23 37.74392499492768 40.0 37.5 41.0 33.0 41.0 24-25 37.586376119532765 40.0 37.0 41.0 32.0 41.0 26-27 37.65324555809977 40.0 37.0 41.0 33.0 41.0 28-29 37.58215703892641 40.0 37.0 41.0 33.0 41.0 30-31 37.325779506391115 39.0 37.0 41.0 32.0 41.0 32-33 37.01539085823599 39.0 35.5 41.0 31.5 41.0 34-35 36.710046447929045 39.0 35.0 40.5 31.0 41.0 36-37 36.661057831657054 39.0 35.0 41.0 31.0 41.0 38-39 36.556824439871306 39.0 35.0 41.0 30.5 41.0 40-41 36.32015104634648 39.0 35.0 41.0 30.0 41.0 42-43 36.002280730993306 39.0 35.0 41.0 30.0 41.0 44-45 35.63650510130141 39.0 35.0 40.5 28.0 41.0 46-47 35.07194447262398 39.0 35.0 40.0 25.0 41.0 48-49 34.57567353120199 38.5 34.5 40.0 23.0 41.0 50-51 33.62253177444132 37.5 33.5 39.5 20.5 40.5 52-53 33.703542107475144 38.0 33.5 39.5 20.0 40.5 54-55 33.98797408046144 38.0 34.0 40.0 18.5 41.0 56-57 33.71873822497899 38.0 34.0 40.0 15.5 41.0 58-59 33.492305476652845 38.0 33.5 40.0 13.5 41.0 60-61 33.15847185588824 38.0 33.0 40.0 9.0 41.0 62-63 32.78946896466769 37.0 32.5 40.0 7.5 41.0 64-65 32.40084073650039 36.5 32.0 39.5 6.0 41.0 66-67 31.98830559259152 36.0 31.5 39.0 2.0 41.0 68-69 31.518543846555172 35.5 31.0 39.0 2.0 40.0 70-71 31.04540629257123 35.0 31.0 38.5 2.0 40.0 72-73 30.553803694095823 35.0 30.0 37.5 2.0 40.0 74-75 30.066087758325846 35.0 29.5 37.0 2.0 39.0 76-77 29.594224623923942 34.5 29.0 36.5 2.0 39.0 78-79 29.052662422828323 34.0 29.0 36.0 2.0 38.5 80-81 28.508308635981567 34.0 27.5 35.5 2.0 37.5 82-83 27.97510488826411 34.0 26.5 35.0 2.0 37.0 84-85 27.48976660096809 33.5 26.0 35.0 2.0 36.5 86-87 26.961367061824294 33.0 25.5 35.0 2.0 36.0 88-89 26.41015658966407 33.0 24.5 35.0 2.0 36.0 90-91 25.85386293150923 32.0 22.0 35.0 2.0 35.0 92-93 25.28835124344222 32.0 19.5 35.0 2.0 35.0 94-95 24.6504956378076 32.0 15.0 34.0 2.0 35.0 96-97 23.850702697023273 31.0 2.0 34.0 2.0 35.0 98-99 22.91636655459262 31.0 2.0 34.0 2.0 35.0 100-101 20.5063240920553 28.0 2.0 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.21444916613716813 1101 2 0.3471029531586609 1101 3 0.3903019714737077 1101 4 0.29228718710998436 1101 5 0.25248041415414235 1101 6 0.34888140856038774 1101 7 0.37105744858507705 1101 8 0.4324018377263883 1101 9 0.4644315960856815 1101 10-11 0.40669136804775263 1101 12-13 0.40456151558094433 1101 14-15 0.7460920194172829 1101 16-17 0.797452283869589 1101 18-19 0.75197813687614 1101 20-21 0.8557802822102047 1101 22-23 0.8008446114593255 1101 24-25 0.5348909824722696 1101 26-27 0.6158345661881839 1101 28-29 0.6417788696913647 1101 30-31 0.7077597514646712 1101 32-33 0.712626415369968 1101 34-35 0.8841476788920843 1101 36-37 1.0045617729596685 1101 38-39 1.1194994915869998 1101 40-41 1.2500007307460024 1101 42-43 1.2159213719603272 1101 44-45 1.489121225431326 1101 46-47 1.6764555176019087 1101 48-49 1.589892259289833 1101 50-51 1.5550291247427879 1101 52-53 1.5553530068827826 1101 54-55 1.5709963358330725 1101 56-57 1.6336227337595162 1101 58-59 1.6705602261070283 1101 60-61 1.6860147125742344 1101 62-63 1.6094307044062646 1101 64-65 1.595496423173806 1101 66-67 1.7385857370873588 1101 68-69 2.02020578357973 1101 70-71 1.9642847670836403 1101 72-73 1.7486944892008616 1101 74-75 1.840961484704902 1101 76-77 1.6989565741752308 1101 78-79 1.8089333193706203 1101 80-81 2.043023701328895 1101 82-83 2.005970941422671 1101 84-85 2.160370289529183 1101 86-87 2.113338764720293 1101 88-89 2.2351105791242105 1101 90-91 2.238585477079493 1101 92-93 2.51952802499461 1101 94-95 2.8220107677103456 1101 96-97 2.7923131997936146 1101 98-99 3.0134981497942803 1101 100-101 3.1427591990809347 1102 1 0.023511889357738625 1102 2 0.06658302044165865 1102 3 -0.003198613540206452 1102 4 0.1204646319432996 1102 5 0.14712670475628187 1102 6 0.018242296859853013 1102 7 -0.11494702158689307 1102 8 -0.015309183321313924 1102 9 -0.11172390240505337 1102 10-11 0.034902864019223045 1102 12-13 -0.10271851534670873 1102 14-15 0.1747561651049523 1102 16-17 0.042102030790267975 1102 18-19 0.26377954449387886 1102 20-21 0.10544553135957102 1102 22-23 -0.021197227916587735 1102 24-25 0.03486409999478468 1102 26-27 0.09837887747190877 1102 28-29 -0.003200891643437842 1102 30-31 0.04313145932126616 1102 32-33 0.007734572537735573 1102 34-35 0.17252881609768878 1102 36-37 0.06731571636309752 1102 38-39 0.1833773785730628 1102 40-41 0.04064511653259473 1102 42-43 0.28626769507746985 1102 44-45 0.24512433867824512 1102 46-47 0.21390792921616963 1102 48-49 0.2666035850056119 1102 50-51 0.16524984268416176 1102 52-53 0.1016663951246457 1102 54-55 0.21268491385242072 1102 56-57 0.5296822746067917 1102 58-59 0.2777957089291263 1102 60-61 0.03092457946757321 1102 62-63 0.35045387710186304 1102 64-65 0.23567958245376275 1102 66-67 -0.04777921430655496 1102 68-69 0.27748005397648967 1102 70-71 0.21578186505618646 1102 72-73 0.13659327795323506 1102 74-75 0.005963571297204595 1102 76-77 0.30288807250211747 1102 78-79 0.3026082564528245 1102 80-81 0.6980203858233374 1102 82-83 0.5279057629358199 1102 84-85 0.6461904227126567 1102 86-87 0.033849839020899 1102 88-89 0.05594270701311643 1102 90-91 0.443647575602494 1102 92-93 0.7871814919381244 1102 94-95 0.510022974915362 1102 96-97 0.5039755865991822 1102 98-99 1.06894005932066 1102 100-101 1.0218328545090216 1103 1 0.1684595708456058 1103 2 0.3202007898976973 1103 3 0.24638323795856465 1103 4 0.2785533079681244 1103 5 0.373666147719689 1103 6 0.2223701218597114 1103 7 0.3436148900795928 1103 8 0.4081596510576304 1103 9 0.1161370610775947 1103 10-11 0.24865095880335275 1103 12-13 0.3968447834881843 1103 14-15 0.6602265750331924 1103 16-17 0.4147936512655548 1103 18-19 0.3097416285574255 1103 20-21 0.3822498492780184 1103 22-23 0.3283249696321775 1103 24-25 0.3547405677959219 1103 26-27 0.25595093316415074 1103 28-29 0.2936103335744846 1103 30-31 0.40213264098324686 1103 32-33 0.36452227868637266 1103 34-35 0.31517168588082 1103 36-37 0.33581985788852364 1103 38-39 0.5485523159221515 1103 40-41 0.5604507987854035 1103 42-43 0.21340139413613457 1103 44-45 0.33633636993805993 1103 46-47 0.5415023051901784 1103 48-49 0.5064823932966576 1103 50-51 0.47224505324261656 1103 52-53 0.5372315663514584 1103 54-55 0.4913202369827445 1103 56-57 0.7375620190937937 1103 58-59 0.5132604103454099 1103 60-61 0.7150854567516873 1103 62-63 0.6424942134473852 1103 64-65 0.5342875618117873 1103 66-67 0.7234364704009089 1103 68-69 1.0885111824922156 1103 70-71 0.9435229497876279 1103 72-73 1.0496526407691285 1103 74-75 0.970652143427106 1103 76-77 0.884201544019497 1103 78-79 0.7045474379663794 1103 80-81 0.916152617794431 1103 82-83 0.7807129236932262 1103 84-85 0.8616325184215725 1103 86-87 0.6666754953570617 1103 88-89 0.8711795817169872 1103 90-91 0.8404219809182578 1103 92-93 0.8628050041571917 1103 94-95 0.9386437413401758 1103 96-97 0.8172301805241844 1103 98-99 1.1397798385154196 1103 100-101 1.2090178160051082 1104 1 0.18378539876901812 1104 2 0.3385739786012323 1104 3 0.22713350503063268 1104 4 0.3783355415123708 1104 5 0.17147204795799809 1104 6 0.28225589719046695 1104 7 0.4427260194520386 1104 8 0.3562860377779842 1104 9 0.254220168426869 1104 10-11 0.31152588689933935 1104 12-13 0.36729907992448574 1104 14-15 0.4258331710284793 1104 16-17 0.3006648540485841 1104 18-19 0.3728733567191398 1104 20-21 0.6206253663021428 1104 22-23 0.40702220577866655 1104 24-25 0.5760063524399683 1104 26-27 0.4930013618490605 1104 28-29 0.4991941942039446 1104 30-31 0.6814249684820837 1104 32-33 0.8688399437916914 1104 34-35 0.8069369637727348 1104 36-37 0.6628530296090176 1104 38-39 0.5734961623077766 1104 40-41 0.6451913964789995 1104 42-43 0.944781677369491 1104 44-45 0.8378908663360036 1104 46-47 0.4780624169495695 1104 48-49 0.45999815739669003 1104 50-51 0.2832367808565053 1104 52-53 0.513253288213015 1104 54-55 0.6837388142418916 1104 56-57 0.5872723486196421 1104 58-59 1.0372523844873598 1104 60-61 1.0610807453264854 1104 62-63 0.9964194880522612 1104 64-65 0.8886065965174552 1104 66-67 0.9734227743974024 1104 68-69 1.0165831960786598 1104 70-71 0.7383191532554605 1104 72-73 0.6979153027243719 1104 74-75 0.7433635412412265 1104 76-77 1.180846707960658 1104 78-79 1.1071003730199287 1104 80-81 1.1039796098781416 1104 82-83 1.2679105844158265 1104 84-85 1.202861597776213 1104 86-87 1.3153867014271299 1104 88-89 1.1661401372268898 1104 90-91 1.1985895926091672 1104 92-93 1.2082250605847271 1104 94-95 1.1799159631058664 1104 96-97 1.0126276385459327 1104 98-99 1.1749374795157763 1104 100-101 1.2632950779000858 1105 1 0.32392252468396165 1105 2 0.3637368928357674 1105 3 0.44966199571904397 1105 4 0.2696771518153511 1105 5 0.37887499431211324 1105 6 0.29279513558572035 1105 7 0.4607083536810137 1105 8 0.19249932416731497 1105 9 0.3411592338705205 1105 10-11 0.2954390713707511 1105 12-13 0.43829030880740305 1105 14-15 -0.1830645713353931 1105 16-17 0.3075829182259824 1105 18-19 0.4763895246057359 1105 20-21 0.523529254624826 1105 22-23 0.24318144479160964 1105 24-25 0.42522189337699245 1105 26-27 0.36253871630720624 1105 28-29 0.35521198351182903 1105 30-31 0.4657720761753339 1105 32-33 0.2568962457157795 1105 34-35 0.5889986395502476 1105 36-37 0.47235146995615906 1105 38-39 0.4811716618599533 1105 40-41 0.7388361971213868 1105 42-43 0.6132859548332803 1105 44-45 0.6425250428084084 1105 46-47 0.7941700793233295 1105 48-49 0.6656020982225357 1105 50-51 0.5743243684904655 1105 52-53 0.15755592263656837 1105 54-55 0.4352019539311982 1105 56-57 0.5905036096221465 1105 58-59 0.47100188261886444 1105 60-61 0.3615455527634026 1105 62-63 0.3854833874932595 1105 64-65 0.47859161767313907 1105 66-67 0.5646933510056975 1105 68-69 0.7011665680172605 1105 70-71 0.4274811100793272 1105 72-73 0.6459564713830694 1105 74-75 0.695473705236406 1105 76-77 0.5504728545371442 1105 78-79 0.7014362079746839 1105 80-81 0.8575390156658429 1105 82-83 0.5708808738009203 1105 84-85 0.37803686569678163 1105 86-87 0.47576070927172154 1105 88-89 0.7610511906874677 1105 90-91 0.6425386027700739 1105 92-93 0.7554006991158708 1105 94-95 0.8054873210848008 1105 96-97 0.9710072982018829 1105 98-99 1.076594417654693 1105 100-101 1.5450517720537036 1106 1 0.28924899174149843 1106 2 0.13312223356890485 1106 3 0.23604484858704566 1106 4 0.0848705267870784 1106 5 -0.23296585190998087 1106 6 -0.23975588304716666 1106 7 -0.2426180785812022 1106 8 -0.20060946338988117 1106 9 -0.2296666329678061 1106 10-11 -0.3993886048240327 1106 12-13 -0.4904706299702397 1106 14-15 -0.2364666834111091 1106 16-17 -0.33931855883520967 1106 18-19 -0.5560136139318175 1106 20-21 -0.2907985868477141 1106 22-23 -0.35099207945202693 1106 24-25 -0.40859132536376563 1106 26-27 -0.3207151677200102 1106 28-29 -0.23976183301245868 1106 30-31 -0.09929081635932135 1106 32-33 0.0532827520920236 1106 34-35 -0.0317237112213391 1106 36-37 0.12703839909548975 1106 38-39 -0.11585507834082165 1106 40-41 -0.04062238775308202 1106 42-43 -0.24156103276219199 1106 44-45 -0.4547278669499306 1106 46-47 -0.15812119227151555 1106 48-49 0.18213857241310905 1106 50-51 0.3436401185904998 1106 52-53 0.37141228756984646 1106 54-55 0.5693596463058128 1106 56-57 0.6516955231905754 1106 58-59 0.38644926340140984 1106 60-61 0.630221938921828 1106 62-63 0.47395917865176074 1106 64-65 0.5088504948282733 1106 66-67 0.7322241013667004 1106 68-69 0.6499783079570598 1106 70-71 0.18773268021798728 1106 72-73 0.40224572484955345 1106 74-75 0.5224713913478602 1106 76-77 0.5447011814043456 1106 78-79 0.7602011482137279 1106 80-81 0.6995247843813708 1106 82-83 0.6789029340316155 1106 84-85 0.8370479828004207 1106 86-87 0.43289287928163844 1106 88-89 0.7239115396844937 1106 90-91 0.7888920535955926 1106 92-93 0.6863232969342086 1106 94-95 0.8546096092248199 1106 96-97 0.862780815486456 1106 98-99 1.1310577868851937 1106 100-101 1.145645032200889 1107 1 0.5276654477286264 1107 2 0.4426947456350945 1107 3 0.4050404817748152 1107 4 0.4335374998967083 1107 5 0.3360987309445349 1107 6 0.2727699730778639 1107 7 0.3934196139032835 1107 8 0.185600603261463 1107 9 0.6090141300751739 1107 10-11 0.3189922271686143 1107 12-13 0.5020196021603027 1107 14-15 0.6881138727828642 1107 16-17 0.6289186280183898 1107 18-19 0.7076958781389706 1107 20-21 0.8943197767468618 1107 22-23 0.7974852820740566 1107 24-25 0.7244355650062673 1107 26-27 0.8482747196622 1107 28-29 0.6567217339836304 1107 30-31 0.5708884527742057 1107 32-33 0.7741745011152332 1107 34-35 0.7501540180363051 1107 36-37 1.1192585783646294 1107 38-39 1.1990265580646096 1107 40-41 1.4001383569033123 1107 42-43 1.382398976431098 1107 44-45 1.3516097565848852 1107 46-47 1.1033072149911902 1107 48-49 0.6793576025211294 1107 50-51 0.5225472663726123 1107 52-53 0.48652950200330736 1107 54-55 0.4995078880893047 1107 56-57 0.6035514183870916 1107 58-59 0.7069790266742189 1107 60-61 0.7602035523440236 1107 62-63 0.7599853795251263 1107 64-65 0.4806098604913416 1107 66-67 0.7672620236834113 1107 68-69 0.3342290549117841 1107 70-71 0.4004366563154349 1107 72-73 0.5027398641278786 1107 74-75 0.31469157061698994 1107 76-77 0.31677082056775774 1107 78-79 0.33783272716109636 1107 80-81 0.6030412173852788 1107 82-83 0.9841488545096659 1107 84-85 0.8698852615869015 1107 86-87 0.919344269766583 1107 88-89 0.9719234909600587 1107 90-91 1.275711175636502 1107 92-93 1.2907016007724046 1107 94-95 1.3842407285077414 1107 96-97 1.5038863084765772 1107 98-99 1.6080056149706934 1107 100-101 1.9244027890383961 1108 1 0.3814598173736705 1108 2 0.6319848313144121 1108 3 0.3259707143329571 1108 4 0.28647507639609415 1108 5 0.49007669912078455 1108 6 0.32760473439977034 1108 7 0.30590431402568186 1108 8 0.473727898243105 1108 9 0.5255379978842356 1108 10-11 0.5946654217830556 1108 12-13 0.5519890024051008 1108 14-15 0.8010269694780945 1108 16-17 0.6456260943586614 1108 18-19 0.6368268450912353 1108 20-21 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84-85 0.3558159257788702 2315 86-87 0.44023781344946755 2315 88-89 0.46144357580693907 2315 90-91 0.44816245496833673 2315 92-93 0.4249717850492196 2315 94-95 0.4332232288391751 2315 96-97 0.41793244396643203 2315 98-99 0.05181444434892413 2315 100-101 0.6468109820650412 2316 1 -0.34852650273576913 2316 2 -0.37411639987573864 2316 3 -0.5514301064604759 2316 4 -2.4688464010320885 2316 5 -0.8396907427396769 2316 6 -0.5156510795537201 2316 7 -0.5686237297499659 2316 8 -0.16591642460231526 2316 9 -0.4947629597081047 2316 10-11 -0.36610065208835607 2316 12-13 -0.5400082616167836 2316 14-15 -0.5955393779911304 2316 16-17 -0.49210304380823544 2316 18-19 -0.5147189825421421 2316 20-21 -0.47072666288161713 2316 22-23 -0.5914775662231619 2316 24-25 -0.3675458591423464 2316 26-27 -0.12923302027588335 2316 28-29 -0.41389746106280967 2316 30-31 -0.6778112376282692 2316 32-33 -0.6418007310829026 2316 34-35 -0.27631657019899336 2316 36-37 -0.6785742389728355 2316 38-39 -1.0488681787774965 2316 40-41 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Count 2 2103.0 3 413.0 4 336.0 5 416.0 6 446.0 7 577.0 8 670.0 9 938.0 10 1160.0 11 1614.0 12 2301.0 13 3320.0 14 3946.0 15 5173.0 16 7094.0 17 9618.0 18 8157.0 19 4603.0 20 4168.0 21 4297.0 22 4537.0 23 5019.0 24 5587.0 25 6406.0 26 7239.0 27 8419.0 28 10038.0 29 11925.0 30 14280.0 31 17775.0 32 22369.0 33 29302.0 34 39706.0 35 54976.0 36 75540.0 37 96611.0 38 72271.0 39 8664.0 40 2.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 46.85214187345341 18.763240226305673 14.337053011776762 20.04756488846415 2 14.69467069837551 21.048970733789172 41.512980468005125 22.743378099830196 3 20.12991493967327 27.35404999019373 30.303013794155465 22.21302127597754 4 11.768658559618766 16.64959537404292 38.69716979761438 32.88457626872394 5 12.111647267569516 39.596133135498256 33.57953750905786 14.712682087874368 6 28.864667658910225 36.73285657240353 18.485679021309036 15.91679674737721 7 26.788812661076626 35.66481541979745 20.37769066027805 17.16868125884787 8 26.171579365756358 33.551426803173214 19.906277280589197 20.370716550481227 9 27.48173075875889 16.96126158504046 21.34587948290696 34.211128173293694 10-11 24.482940766449328 27.38462599654386 26.57078184220844 21.561651394798368 12-13 27.010013994543762 25.444385755696285 27.497281863906238 20.04831838585371 14-15 23.48916358091919 25.616514194438395 28.50432004646773 22.39000217817469 16-17 20.003502747751345 29.712738386485565 28.96091804659199 21.322840819171102 18-19 19.766066905699862 27.67158617673029 32.988577407642346 19.573769509927498 20-21 21.68657971890338 25.83896606387603 32.4996075463617 19.974846670858888 22-23 20.742097922151228 26.105828085850902 32.457618582213286 20.69445540978459 24-25 20.61880649025373 26.106029256960323 33.14639369850085 20.12877055428509 26-27 20.625024955262024 25.93069515377886 33.50230314381854 19.94197674714058 28-29 19.68641067550484 26.652460733269383 33.60181216404223 20.05931642718355 30-31 19.864641553300793 26.693908960645686 33.229064859709446 20.21238462634408 32-33 19.705375463867565 26.713870967449182 33.063853769522375 20.516899799160885 34-35 20.483992017562088 26.374352379802872 32.81611443309354 20.3255411695415 36-37 20.56854715404292 26.035423493040614 33.296250488867436 20.09977886404903 38-39 20.523659683811683 26.32171580796423 33.251854594021744 19.90276991420234 40-41 20.15649398312935 26.750508777835343 33.11751293606775 19.975484302967562 42-43 19.895321688899617 27.063735262598648 32.337848886114244 20.703094162387494 44-45 20.124426731420836 27.251831646421916 31.805766532911562 20.817975089245692 46-47 20.580690414896832 27.519411850643312 30.91105257283584 20.988845161624017 48-49 20.904836193447736 27.524800817512627 30.67215184244368 20.89821114659595 50-51 20.75001860345244 28.046927889388407 30.64043065631165 20.5626228508475 52-53 20.71776822600905 28.368480857335783 30.277392560753363 20.636358355901805 54-55 19.98083555874963 28.053765694742754 30.482622697340663 21.482776049166954 56-57 20.90654549875608 28.515227344074646 29.616971007891763 20.96125614927751 58-59 21.05401165539023 27.907428292493496 29.848087966167974 21.190472085948294 60-61 20.77934213522544 27.69393511348925 30.81651112302599 20.710211628259316 62-63 21.03576548295197 27.69992197281751 30.567420929430767 20.69689161479976 64-65 20.603834799160243 28.211376655519487 30.66151827853191 20.52327026678836 66-67 20.462743874114505 28.167550952270354 30.320592846359308 21.049112327255838 68-69 20.28704904185429 28.35704843396865 30.273521318186397 21.082381205990668 70-71 20.551278619807377 28.443291158764794 29.720686104167704 21.28474411726012 72-73 20.59344154907406 28.075697185852416 29.971211624950893 21.35964964012263 74-75 20.278705616851916 28.74694992754235 29.79230016070445 21.182044294901285 76-77 20.527668192194763 28.147989533014194 29.93425469907416 21.390087575716883 78-79 20.51848807129007 28.212694256090025 30.05791728254252 21.210900390077388 80-81 20.311884718610553 28.729070225325685 29.747998889431273 21.211046166632492 82-83 20.29885434932684 28.67927677682899 29.555138464162244 21.466730409681922 84-85 20.320315689209416 28.373293418605705 29.43502517349299 21.871365718691884 86-87 20.469610828826916 28.479375714971283 29.540957818848845 21.510055637352956 88-89 20.76131756312333 27.943918196193774 29.327546366649216 21.96721787403368 90-91 20.26913738853989 27.961745183217385 29.873760193360845 21.89535723488189 92-93 20.414597369323534 28.29102198641658 29.787176132662445 21.50720451159744 94-95 20.05031608590569 28.242783733370107 29.643214858577867 22.063685322146338 96-97 19.852910488792272 28.06794860395614 29.531403955412244 22.547736951839344 98-99 20.136539829561126 28.139653895849364 29.11724568458646 22.60656059000305 100-101 20.54039048261009 27.500074845070703 29.919248972808106 22.040285699511102 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 802.0 1 511.0 2 327.5 3 423.5 4 348.0 5 337.5 6 599.0 7 1222.5 8 1939.0 9 2231.0 10 1941.0 11 1589.0 12 1583.0 13 1686.0 14 1727.5 15 1769.5 16 1838.5 17 1880.0 18 1890.0 19 1965.0 20 2174.0 21 2451.0 22 2820.0 23 3219.5 24 3712.0 25 4322.5 26 5104.0 27 6044.0 28 6722.0 29 7524.5 30 8491.0 31 9257.0 32 10229.0 33 11326.0 34 12410.0 35 13295.5 36 14338.5 37 15525.5 38 15854.0 39 15686.5 40 15805.5 41 16336.5 42 16723.0 43 17125.0 44 18123.5 45 18887.5 46 19312.5 47 21344.0 48 28660.5 49 28497.0 50 20832.0 51 17371.5 52 14832.0 53 13520.0 54 12762.5 55 11689.5 56 10352.0 57 9127.5 58 8250.5 59 7428.0 60 6504.5 61 5417.5 62 4276.5 63 3169.5 64 2284.0 65 1754.0 66 1306.5 67 939.0 68 663.5 69 463.0 70 332.5 71 245.0 72 206.5 73 178.0 74 147.5 75 119.0 76 91.0 77 69.0 78 52.5 79 29.0 80 17.5 81 16.5 82 11.5 83 5.0 84 5.0 85 6.0 86 2.5 87 0.5 88 1.0 89 1.0 90 0.5 91 0.5 92 1.5 93 1.5 94 0.0 95 0.5 96 0.5 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.291658212805426 2 0.2501739080026666 3 0.2441958204110026 4 0.2507173705109997 5 0.2516231413582215 6 0.19492188632213558 7 0.18767571954436102 8 0.18640764035825047 9 0.19745804469435668 10-11 0.2014434364221327 12-13 0.1969145821860236 14-15 0.19854496971102287 16-17 0.18477725283325122 18-19 0.18957783832352684 20-21 0.1803389756818643 22-23 0.18794745079852757 24-25 0.18767571954436102 26-27 0.18658879452769483 28-29 0.1942878467290803 30-31 0.18740398829019447 32-33 0.19519361757630213 34-35 0.19138938001797048 36-37 0.18070128402075303 38-39 0.17245876931103446 40-41 0.17037549636242427 42-43 0.18015782151241994 44-45 0.18278455696936322 46-47 0.18106359235964176 48-49 0.19501246340685777 50-51 0.18894379873047157 52-53 0.19926958638880032 54-55 0.1803389756818643 56-57 0.16937914843048027 58-59 0.17037549636242427 60-61 0.1602308628735399 62-63 0.167748760905481 64-65 0.16394452334714937 66-67 0.16521260253325992 68-69 0.1676581838207588 70-71 0.16910741717631372 72-73 0.1676581838207588 74-75 0.18196936320686358 76-77 0.17209646097214573 78-79 0.17599127561519956 80-81 0.17155299846381264 82-83 0.15298469609576534 84-85 0.15235065650271007 86-87 0.15533970029854208 88-89 0.16168009622909482 90-91 0.16702414422770354 92-93 0.16358221500826065 94-95 0.16303875249992755 96-97 0.1672052983971479 98-99 0.17535723602214429 100-101 0.15896278368742936 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 552016.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 61.090562895122446 #Duplication Level Percentage of deduplicated Percentage of total 1 81.36492524807639 49.706290833245475 2 11.291184443566186 13.795696268202166 3 3.2361245258094438 5.930900066412305 4 1.3751608174023326 3.360373936257008 5 0.7198608663063605 2.198835276441303 6 0.43880688117486216 1.6084175623935264 7 0.298865052396054 1.278048399638864 8 0.20289332913051825 0.9915894147398965 9 0.1650352349545739 0.9073885860813335 >10 0.8246014578213005 8.930615991943975 >50 0.04916770501641566 2.055247132059601 >100 0.026520759042415885 3.2616350356593133 >500 0.004171804793188917 1.7659832271546918 >1k 0.0023838884532508095 3.1800805066658397 >5k 2.979860566563512E-4 1.0288977631046612 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5652 1.023883365699545 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4497 0.8146502999913047 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2919 0.5287890206080983 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 2132 0.3862206892553839 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 2092 0.3789745224776093 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 1857 0.3364032926581838 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 1519 0.27517318338598884 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 1315 0.23821773281933858 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1138 0.20615344482768616 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 972 0.17608185269992174 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 904 0.163763369177705 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 850 0.15398104402770935 No Hit CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG 840 0.1521695023332657 No Hit CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA 761 0.13785832294716097 No Hit GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT 733 0.13278600620271877 No Hit NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 697 0.12626445610272166 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 667 0.12082983101939075 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 609 0.11032288919161763 No Hit TGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC 608 0.11014173502217327 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 1.8115416944436394E-4 0.0 0.0 0.0 0.0 16-17 2.7173125416654593E-4 0.0 0.0 0.0 0.0 18-19 3.623083388887279E-4 0.0 0.0 0.0 0.0 20-21 3.623083388887279E-4 0.0 0.0 0.0 0.0 22-23 3.623083388887279E-4 0.0 0.0 2.7173125416654593E-4 0.0 24-25 5.434625083330917E-4 0.0 0.0 5.434625083330917E-4 0.0 26-27 5.434625083330917E-4 0.0 0.0 6.340395930552738E-4 0.0 28-29 5.434625083330917E-4 0.0 0.0 0.0028078896263876406 0.0 30-31 5.434625083330917E-4 0.0 0.0 0.007246166777774557 0.0 32-33 5.434625083330917E-4 0.0 0.0 0.013767716877771658 0.0 34-35 5.434625083330917E-4 0.0 0.0 0.02101388365554622 0.0 36-37 7.246166777774558E-4 0.0 0.0 0.028531781687487316 0.0 38-39 7.246166777774558E-4 0.0 0.0 0.035777948465261875 0.0 40-41 8.151937624996377E-4 0.0 0.0 0.043929886090258254 0.0 42-43 9.963479319440016E-4 0.0 0.0 0.05380278832497609 0.0 44-45 0.0010869250166661835 0.0 0.0 0.060686646763861915 0.0 46-47 0.0010869250166661835 0.0 0.0 0.0743637865569114 0.0 48-49 0.0010869250166661835 0.0 0.0 0.08849381177357178 0.0 50-51 0.0010869250166661835 0.0 0.0 0.09854786817773398 0.0 52-53 0.0010869250166661835 0.0 0.0 0.12055809976522419 0.0 54-55 0.0010869250166661835 0.0 0.0 0.16267644416103882 0.0 56-57 0.0012680791861105476 0.0 0.0 0.24664140169850152 0.0 58-59 0.0012680791861105476 0.0 0.0 0.33042520506651984 0.0 60-61 0.0012680791861105476 0.0 0.0 0.41602055012898176 0.0 62-63 0.0012680791861105476 0.0 0.0 0.5120322599344946 0.0 64-65 0.0012680791861105476 0.0 0.0 0.6190037969913915 0.0 66-67 0.0012680791861105476 0.0 0.0 0.7822237036607635 0.0 68-69 0.0012680791861105476 0.0 0.0 0.9738848149329005 0.0 70-71 0.0012680791861105476 0.0 0.0 1.1795853743369757 0.0 72-73 0.0013586562708327297 0.0 0.0 1.3860105504188285 0.0 74-75 0.0014492333555549115 0.0 0.0 1.6010405495492885 0.0 76-77 0.0014492333555549115 0.0 0.0 1.8415227094866815 0.0 78-79 0.0014492333555549115 0.0 0.0 2.04278499173937 0.0 80-81 0.002445581287498913 0.0 0.0 2.204193356714298 0.0 82-83 0.002717312541665459 0.0 0.0 2.4024665951711546 0.0 84-85 0.002717312541665459 0.0 0.0 2.70780194776963 0.0 86-87 0.002717312541665459 0.0 0.0 3.077537607605577 0.0 88-89 0.002717312541665459 0.0 0.0 3.5334120750123184 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position ATCAGCG 25 0.0016173314 37.99261 94-95 GTAAGCG 45 2.4160827E-5 31.660505 94-95 TACCTGG 325 0.0 30.712833 2 CATTCCG 100 4.9443246E-5 28.503517 9 TAAGGCG 95 0.0012209745 25.016718 5 GACAGTG 305 0.0 24.92337 7 GTACATG 4950 0.0 24.20679 1 GAGTACT 4220 0.0 23.804892 12-13 GTACTAG 100 0.0016513688 23.75293 6 TACATGG 5085 0.0 23.27514 2 GTGTAAG 165 2.4043948E-6 23.054085 1 GTATAGA 125 2.2381305E-4 22.823544 1 ATGGGAT 1370 0.0 22.551565 5 AGTACTT 4280 0.0 22.194967 12-13 GTACTTT 4545 0.0 21.997168 14-15 ACATGGG 5210 0.0 21.984863 3 GTACCTG 485 0.0 21.56864 1 GTATAGC 245 7.408744E-9 21.348553 1 CATGGGA 3760 0.0 21.111183 4 GTATCAA 9325 0.0 20.906284 1 >>END_MODULE