##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937698_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 552016 Sequences flagged as poor quality 0 Sequence length 101 %GC 41 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.31762303991189 33.0 31.0 34.0 31.0 34.0 2 32.492259282339646 34.0 31.0 34.0 31.0 34.0 3 32.555579910727225 34.0 31.0 34.0 31.0 34.0 4 36.16934654067998 37.0 35.0 37.0 35.0 37.0 5 36.11715240137967 37.0 35.0 37.0 35.0 37.0 6 36.1985504043361 37.0 36.0 37.0 35.0 37.0 7 36.152705718674824 37.0 36.0 37.0 35.0 37.0 8 36.144709573635545 37.0 36.0 37.0 35.0 37.0 9 37.805163256137504 39.0 38.0 39.0 35.0 39.0 10-11 37.750604149155095 39.0 37.0 39.0 35.0 39.0 12-13 37.65826624590592 39.0 37.0 39.0 35.0 39.0 14-15 38.77250478247007 40.0 38.0 41.0 35.0 41.0 16-17 38.760649147850785 40.0 38.0 41.0 35.0 41.0 18-19 38.86710801860816 40.0 38.0 41.0 35.0 41.0 20-21 38.826078410770705 40.0 38.0 41.0 35.0 41.0 22-23 38.67705646213153 40.0 38.0 41.0 34.5 41.0 24-25 38.723979920871855 40.0 38.0 41.0 35.0 41.0 26-27 38.56485500420278 40.0 38.0 41.0 34.5 41.0 28-29 38.39801473145706 40.0 38.0 41.0 34.0 41.0 30-31 38.32223431929509 40.0 37.5 41.0 34.0 41.0 32-33 38.11919491464016 40.0 37.0 41.0 34.0 41.0 34-35 37.921633612069215 40.0 37.0 41.0 34.0 41.0 36-37 37.69824244224805 40.0 36.0 41.0 33.0 41.0 38-39 37.49743032810643 40.0 36.0 41.0 33.0 41.0 40-41 37.25566650242022 40.0 36.0 41.0 33.0 41.0 42-43 36.93293219761746 40.0 35.5 41.0 32.5 41.0 44-45 36.425086410538825 39.5 35.0 41.0 31.0 41.0 46-47 35.7714522767456 39.0 35.0 41.0 29.0 41.0 48-49 35.184952972377616 39.0 35.0 41.0 25.0 41.0 50-51 34.81558958435987 39.0 35.0 41.0 23.0 41.0 52-53 34.54011206196922 39.0 35.0 40.0 22.0 41.0 54-55 34.30866043737863 38.5 35.0 40.0 20.0 41.0 56-57 34.05213707573694 38.0 34.0 40.0 18.5 41.0 58-59 33.797007695429116 38.0 34.0 40.0 16.5 41.0 60-61 33.416299527549924 38.0 33.5 40.0 12.5 41.0 62-63 33.07313646705893 37.0 33.0 40.0 8.0 41.0 64-65 32.6762847453697 36.5 33.0 39.5 7.0 41.0 66-67 32.24728812208342 36.0 32.0 39.0 2.0 40.5 68-69 31.813625873163097 35.5 32.0 39.0 2.0 40.0 70-71 31.384991558215702 35.0 31.0 38.0 2.0 40.0 72-73 30.892868141503143 35.0 31.0 37.5 2.0 39.5 74-75 30.39989511173589 35.0 30.0 37.0 2.0 39.0 76-77 29.186150401437637 33.5 28.5 35.5 2.0 38.0 78-79 29.734596460972146 34.0 30.0 36.0 2.0 38.5 80-81 29.52386796759514 34.0 30.0 36.0 2.0 37.5 82-83 29.17140082896148 34.0 29.5 35.0 2.0 37.0 84-85 28.79194262485145 34.0 29.0 35.0 2.0 36.5 86-87 28.360304773774672 34.0 29.0 35.0 2.0 36.0 88-89 27.980659980870122 34.0 28.5 35.0 2.0 36.0 90-91 27.451843787136603 33.5 27.0 35.0 2.0 35.0 92-93 26.99238790179995 33.0 26.0 35.0 2.0 35.0 94-95 26.540722551520247 33.0 25.0 35.0 2.0 35.0 96-97 25.989679646966756 33.0 24.5 35.0 2.0 35.0 98-99 25.136778644097273 32.0 19.0 34.0 2.0 35.0 100-101 22.634261506912843 29.5 2.0 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.4232348853610972 1101 2 0.508377809643747 1101 3 0.3859150820148969 1101 4 0.2491503522220455 1101 5 0.25485686086159376 1101 6 0.24077826361222066 1101 7 0.25974624178282113 1101 8 0.3520664390964967 1101 9 0.44725714190883537 1101 10-11 0.4230949034506182 1101 12-13 0.42316745692355795 1101 14-15 0.37255754132203833 1101 16-17 0.3721891159801416 1101 18-19 0.3545751667925785 1101 20-21 0.4731744126974746 1101 22-23 0.5275684956891382 1101 24-25 0.41342060879726006 1101 26-27 0.3129453998245353 1101 28-29 0.2614489892180103 1101 30-31 0.2796643067825286 1101 32-33 0.2874952241137194 1101 34-35 0.3281678781978101 1101 36-37 0.37406732033739587 1101 38-39 0.4216162418440632 1101 40-41 0.485316247479318 1101 42-43 0.3931767838696274 1101 44-45 0.4494677571832497 1101 46-47 0.8169195229208199 1101 48-49 0.6216480986982091 1101 50-51 0.8532997917493716 1101 52-53 0.6681762282979449 1101 54-55 0.7985028935139908 1101 56-57 0.6560539206418028 1101 58-59 0.6151599619243555 1101 60-61 0.6408728567332318 1101 62-63 0.6061281364108524 1101 64-65 0.7271331145633866 1101 66-67 0.5952613372802347 1101 68-69 0.6258318544171964 1101 70-71 0.6660747173570876 1101 72-73 0.5775891239631292 1101 74-75 0.6497934750003509 1101 76-77 0.7783366090023343 1101 78-79 0.9939806031212548 1101 80-81 1.1271834154585214 1101 82-83 1.0372622049838647 1101 84-85 0.9925203536890663 1101 86-87 0.9144052443974466 1101 88-89 1.1183074338873418 1101 90-91 0.8490578760939691 1101 92-93 0.8600180801635169 1101 94-95 0.819887060573901 1101 96-97 1.2316139810939184 1101 98-99 1.409329753827123 1101 100-101 1.8548397853607952 1102 1 0.13371316303916814 1102 2 0.06334479540115012 1102 3 -0.05177773575518785 1102 4 -0.030606630181452488 1102 5 -0.18066487682174426 1102 6 -0.015470632286024966 1102 7 -0.003425532127863562 1102 8 0.07756818133706389 1102 9 0.12919390920131235 1102 10-11 0.13786029622922058 1102 12-13 0.07028373515976227 1102 14-15 0.1046787736148076 1102 16-17 -1.9720314302418274E-4 1102 18-19 0.06578439183475382 1102 20-21 0.00574445435251647 1102 22-23 -0.06424412576205185 1102 24-25 -0.07359003410358156 1102 26-27 0.056929485397859025 1102 28-29 -0.10012929383655234 1102 30-31 -0.105713929675737 1102 32-33 0.11112440977947102 1102 34-35 0.1076675568882024 1102 36-37 -0.0918316169086566 1102 38-39 -0.037007784946254674 1102 40-41 0.021196674219446265 1102 42-43 -0.05979040158619142 1102 44-45 -0.18737557429817997 1102 46-47 -0.08331192240918739 1102 48-49 -0.1152159706944289 1102 50-51 -0.35493443911734346 1102 52-53 -0.36891519004716855 1102 54-55 -0.4045903835624287 1102 56-57 -0.05892781207467834 1102 58-59 -0.10808237208914306 1102 60-61 -0.3055145223276199 1102 62-63 -0.3371165530197189 1102 64-65 -0.3539137147124052 1102 66-67 -0.25933518233501474 1102 68-69 -0.30600442361419056 1102 70-71 -0.49471450097727754 1102 72-73 -0.4310235097771944 1102 74-75 -0.4679146529807241 1102 76-77 -0.4232371680369553 1102 78-79 -0.32361026605325094 1102 80-81 -0.43723980178517863 1102 82-83 -0.32052308850878575 1102 84-85 -0.20477910942945599 1102 86-87 -0.34052128963192985 1102 88-89 -0.1512303667163586 1102 90-91 -0.11495416725594865 1102 92-93 -0.09582681050160247 1102 94-95 -0.6450671496061062 1102 96-97 -0.3838782626712671 1102 98-99 -0.42688299153948606 1102 100-101 -0.17395646850195945 1103 1 0.17162909949201577 1103 2 0.21584198395900955 1103 3 0.2073858530721111 1103 4 0.1151659312849489 1103 5 0.07623243275656222 1103 6 0.0908703828015689 1103 7 0.11947587616203492 1103 8 0.0993518629847685 1103 9 0.16261724673991296 1103 10-11 0.225227644682505 1103 12-13 0.1762922294255631 1103 14-15 0.20845238544741562 1103 16-17 0.23453252735811958 1103 18-19 0.22618929658823106 1103 20-21 0.22022323866780624 1103 22-23 0.3177509420836202 1103 24-25 0.26031795610060726 1103 26-27 0.20255077129726118 1103 28-29 0.1662484269829605 1103 30-31 0.12622005709193473 1103 32-33 0.1584051601062768 1103 34-35 0.14282770919130172 1103 36-37 0.1219407390513183 1103 38-39 0.23341869798244375 1103 40-41 0.44234642561374926 1103 42-43 0.3676858081902097 1103 44-45 0.6748223740626145 1103 46-47 0.41566625394958834 1103 48-49 0.5688321634784401 1103 50-51 0.4884140027443138 1103 52-53 0.3940488406189573 1103 54-55 0.3798547776174104 1103 56-57 0.400269171547599 1103 58-59 0.3468803227851822 1103 60-61 0.29599709163996835 1103 62-63 0.2008793026005904 1103 64-65 0.10479519113710722 1103 66-67 0.37843404536040026 1103 68-69 0.510485469465376 1103 70-71 0.41668511198561475 1103 72-73 0.37491990745223447 1103 74-75 0.5977489329923173 1103 76-77 0.35978229228032177 1103 78-79 0.5546742690169921 1103 80-81 0.5902181407529419 1103 82-83 0.6755879877169768 1103 84-85 0.5827229599061283 1103 86-87 0.8351253420605893 1103 88-89 0.8215828450637268 1103 90-91 0.8146755786581856 1103 92-93 1.0336165524969623 1103 94-95 0.966024774562019 1103 96-97 0.9959204551969449 1103 98-99 1.0971213012615095 1103 100-101 1.499993308009799 1104 1 0.19687768195564814 1104 2 0.23404397261872134 1104 3 0.021709133538870162 1104 4 0.12568446197769134 1104 5 0.14881440333754625 1104 6 0.13143109718511425 1104 7 0.03463598244302091 1104 8 0.09105893286177746 1104 9 0.065259205816254 1104 10-11 0.16939246238869288 1104 12-13 0.18034967046426686 1104 14-15 0.24296289028210083 1104 16-17 0.28751209292089897 1104 18-19 0.12249000604121818 1104 20-21 0.12256046166613288 1104 22-23 0.24806567624418818 1104 24-25 0.13485913998314913 1104 26-27 0.2846035283027675 1104 28-29 0.32565278779047446 1104 30-31 0.2769753916852906 1104 32-33 0.2874215131344684 1104 34-35 0.091844336139566 1104 36-37 0.22064098831857848 1104 38-39 0.3309102749402939 1104 40-41 0.2178175681343575 1104 42-43 0.15408293750787294 1104 44-45 0.24086107896852837 1104 46-47 0.23157142282131105 1104 48-49 0.4406615386667596 1104 50-51 0.0452969293063461 1104 52-53 -0.009610046717718035 1104 54-55 -0.21267199806944603 1104 56-57 -0.27966549851567635 1104 58-59 0.023185551919318925 1104 60-61 3.551599556317342E-4 1104 62-63 0.060282978607936855 1104 64-65 -0.07572840707693729 1104 66-67 0.015407776425682584 1104 68-69 -0.006559206898057113 1104 70-71 0.06961468713558716 1104 72-73 0.2563536050992603 1104 74-75 -0.11757621013091679 1104 76-77 -0.13292458585263844 1104 78-79 -0.0548252790148922 1104 80-81 0.28174305378331965 1104 82-83 0.15387297415793455 1104 84-85 -0.0336040458735809 1104 86-87 0.008164074358500528 1104 88-89 -0.06621264621191969 1104 90-91 0.14674887966895156 1104 92-93 0.5164588841045976 1104 94-95 0.4084262318167937 1104 96-97 0.10284857857054774 1104 98-99 0.23389301627506853 1104 100-101 1.1803628261843926 1105 1 0.23090436591747476 1105 2 0.19939188330157265 1105 3 0.0671552560256643 1105 4 0.053786009276869606 1105 5 -0.001259718778634067 1105 6 0.0572240494072318 1105 7 0.14976461519881923 1105 8 0.11186717277035996 1105 9 0.08974266731907932 1105 10-11 0.12001246300637547 1105 12-13 0.26967840204955706 1105 14-15 0.24027210304345914 1105 16-17 0.15415791274238444 1105 18-19 0.11441378379840472 1105 20-21 0.1810513044963642 1105 22-23 0.10686304490906195 1105 24-25 0.15955493046462976 1105 26-27 -0.03435320291190891 1105 28-29 -0.03219103073387686 1105 30-31 -0.10833993615064941 1105 32-33 0.09926715382750473 1105 34-35 0.11997213502154835 1105 36-37 -0.07704423976135644 1105 38-39 -0.27643939614281976 1105 40-41 -0.19868309587626243 1105 42-43 -0.25853017592984884 1105 44-45 0.05660971234200218 1105 46-47 -0.2702121405994262 1105 48-49 -0.551713920735935 1105 50-51 -0.3651118232802091 1105 52-53 -0.26796036636934417 1105 54-55 -0.6226792558599854 1105 56-57 -0.43192853937549813 1105 58-59 -0.3509960163811172 1105 60-61 -0.399711241106786 1105 62-63 -0.5319913349903871 1105 64-65 -0.31730087688761444 1105 66-67 -0.21589183134479129 1105 68-69 -0.20269713580259108 1105 70-71 0.0509220159598236 1105 72-73 -0.270639198878623 1105 74-75 -0.5133226816093455 1105 76-77 -0.5616978648297533 1105 78-79 -0.09855732756487967 1105 80-81 -0.17647832427035937 1105 82-83 -0.06242462613855437 1105 84-85 -0.41012895399304483 1105 86-87 -0.054658279636374374 1105 88-89 0.028771757259466568 1105 90-91 -0.33650415470517814 1105 92-93 -0.23421670810022377 1105 94-95 0.1298042854935595 1105 96-97 0.2534979191925508 1105 98-99 -0.0069922797501362766 1105 100-101 0.2637193226790373 1106 1 -0.11461312742791563 1106 2 -0.08300690582141357 1106 3 -0.08228192972913462 1106 4 -0.06823246703622488 1106 5 -0.0014597918488021833 1106 6 -0.06361138614314399 1106 7 -0.12349170450054459 1106 8 -0.06117863526387879 1106 9 -0.12487484516373826 1106 10-11 -0.1566755447877739 1106 12-13 -0.1546635315261895 1106 14-15 -0.10503226898806872 1106 16-17 0.12442096533550284 1106 18-19 0.07178082219503779 1106 20-21 0.16810309732505146 1106 22-23 0.12965397724956773 1106 24-25 0.05797870262490079 1106 26-27 0.030744484939312144 1106 28-29 0.1550382182092207 1106 30-31 0.14693155430673954 1106 32-33 0.31232757589469173 1106 34-35 0.03819009288442743 1106 36-37 0.37029739805419837 1106 38-39 0.23272047093907133 1106 40-41 0.20380737890582878 1106 42-43 -0.12954828689210984 1106 44-45 -0.0446446588196352 1106 46-47 0.004040195036196792 1106 48-49 0.14683685432973448 1106 50-51 0.4578887508425211 1106 52-53 0.204655719505503 1106 54-55 0.30311538218173695 1106 56-57 0.3494220408364086 1106 58-59 0.4043972576841952 1106 60-61 0.46068994889304093 1106 62-63 0.21161502559348122 1106 64-65 0.21565898671474315 1106 66-67 0.5129874115091226 1106 68-69 -0.014006483564383387 1106 70-71 0.3922510230754668 1106 72-73 0.5487861564685446 1106 74-75 0.7159306108974839 1106 76-77 0.3321155278875878 1106 78-79 0.789949779378528 1106 80-81 0.9736373701571708 1106 82-83 0.6716956612707605 1106 84-85 1.0261434237449159 1106 86-87 1.1304136596805954 1106 88-89 0.595291703674679 1106 90-91 0.5183341340611527 1106 92-93 0.6684129479480738 1106 94-95 0.9125814736840283 1106 96-97 0.8695837940337974 1106 98-99 0.6915323988281301 1106 100-101 0.3695405791379187 1107 1 0.2831689916251747 1107 2 0.30852377678029086 1107 3 0.16356959865515108 1107 4 0.10450943137793445 1107 5 0.15297597004501284 1107 6 0.1896522314160407 1107 7 0.2323920003948814 1107 8 0.2159875334329726 1107 9 0.3241943343352247 1107 10-11 0.3588151470072205 1107 12-13 0.35678785895876075 1107 14-15 0.5165145018692101 1107 16-17 0.322783410750354 1107 18-19 0.5118922908229422 1107 20-21 0.42336625521978277 1107 22-23 0.4495344644938797 1107 24-25 0.34821083316960966 1107 26-27 0.4252400032109662 1107 28-29 0.5238270023967573 1107 30-31 0.3056881619831344 1107 32-33 -0.02699097156565955 1107 34-35 0.10976559276951292 1107 36-37 -0.05559320180790195 1107 38-39 0.09256878167223448 1107 40-41 0.08185265585365897 1107 42-43 0.3223305915266437 1107 44-45 0.2740924131178488 1107 46-47 -0.29526456249080724 1107 48-49 -0.5092364621492322 1107 50-51 -0.853091483586347 1107 52-53 -1.0786124946981275 1107 54-55 -1.1713215288732002 1107 56-57 -1.1641208228730804 1107 58-59 -0.978905431352814 1107 60-61 -1.0395571207461245 1107 62-63 -1.0644824232688492 1107 64-65 -1.1198328542413556 1107 66-67 -1.185398017127973 1107 68-69 -1.1301924178323688 1107 70-71 -1.102600735141035 1107 72-73 -0.8351600627913953 1107 74-75 -1.0687798005021918 1107 76-77 -1.037851146440154 1107 78-79 -1.0600272373992219 1107 80-81 -1.1952663301416102 1107 82-83 -1.2363657039326412 1107 84-85 -1.2283612878156447 1107 86-87 -1.333002796306534 1107 88-89 -0.9192624218137162 1107 90-91 -0.850741940404486 1107 92-93 -0.6961584149570079 1107 94-95 -0.9453477379914474 1107 96-97 -0.7617883043343916 1107 98-99 -0.9090996397836832 1107 100-101 -0.9224727511309894 1108 1 0.1445521005603041 1108 2 0.075904437734998 1108 3 0.19380215679468193 1108 4 0.11638213639629669 1108 5 0.026721380081731638 1108 6 0.17135357780527016 1108 7 0.12572125376085097 1108 8 0.06121397162146991 1108 9 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-1.5856058698589877 2316 86-87 -1.5071049634891942 2316 88-89 -1.2210735673245523 2316 90-91 -1.1717326524787914 2316 92-93 -0.9781398391056122 2316 94-95 -1.2424994407778236 2316 96-97 -1.4422836603715439 2316 98-99 -0.8196569153254814 2316 100-101 -0.21200835484615865 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1.0 3 1.0 4 6.0 5 14.0 6 26.0 7 58.0 8 135.0 9 284.0 10 510.0 11 947.0 12 1704.0 13 2717.0 14 3352.0 15 5041.0 16 7418.0 17 11688.0 18 10264.0 19 4983.0 20 3540.0 21 3236.0 22 3160.0 23 3316.0 24 3727.0 25 4210.0 26 5025.0 27 5905.0 28 7423.0 29 9213.0 30 11247.0 31 14425.0 32 19000.0 33 24910.0 34 35402.0 35 53133.0 36 79324.0 37 116342.0 38 91604.0 39 8724.0 40 1.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 46.462073126758725 19.362700243345156 15.174192005305395 19.001034624590726 2 15.386872844265383 22.208957711370687 40.444842178487576 21.959327265876354 3 20.79432480217965 27.312976435465643 30.799469580591865 21.093229181762847 4 12.034071476189096 17.49188429320889 38.06972986290253 32.40431436769949 5 11.95691170341916 39.99836924024715 33.20226856801174 14.842450488321948 6 28.755144778412216 37.653075273180484 17.476123880467235 16.11565606794006 7 26.476406480971566 36.493507434567114 19.874423929741166 17.155662154720154 8 26.696327642677026 33.8727500652155 19.132235297527608 20.298686994579867 9 27.633619315382163 17.225406509956233 20.774760151879658 34.366214022781946 10-11 24.742670502304282 27.96938856844729 25.70939972754413 21.578541201704297 12-13 27.55777416061819 26.0053242898305 27.09246492517643 19.344436624374886 14-15 23.755017970493608 26.12234065679256 28.46892119069013 21.653720182023708 16-17 19.77497029071621 29.87304715805339 29.181581693284254 21.17040085794615 18-19 19.57696878351352 27.221493579896233 34.01088011941683 19.190657517173413 20-21 21.230000579693343 25.439298860902582 34.058342511811254 19.272358047592824 22-23 20.500155794843554 25.818013507050626 33.67587063955595 20.005960058549878 24-25 20.440349555085362 25.81238587287325 34.41892988609026 19.32833468595113 26-27 20.61313884480772 25.514934457946563 34.75947986637971 19.112446830865995 28-29 19.526183973949628 26.321834395366007 35.03412544213621 19.117856188548163 30-31 19.69562476449958 26.31427349931886 34.52255007101244 19.467551665169125 32-33 19.540103910031593 26.455030288977134 34.358605547665285 19.64626025332599 34-35 20.336367061824294 25.862927886148228 34.21748644966813 19.583218602359352 36-37 20.519695081301993 25.736210544621898 34.41331410683748 19.33078026723863 38-39 20.41926358951877 25.998998216535586 34.48897721169061 19.092760982255026 40-41 20.075052512302687 26.37761576483184 34.2575860916445 19.289745631220974 42-43 19.782238653318466 26.92937296001609 33.45442258094031 19.833965805725136 44-45 20.25312672096461 26.847500797078343 32.70992145155213 20.189451030404914 46-47 20.737442392974117 27.360529404944785 31.78250268108171 20.11952552099939 48-49 21.02520579113649 27.487971363148894 31.287680067244427 20.19914277847019 50-51 20.947309498275413 28.086504738993074 31.150636938059765 19.81554882467175 52-53 20.993775542737893 28.711848931915018 30.28281788933654 20.01155763601055 54-55 20.17504927393409 28.37481159966378 30.648024694936378 20.802114431465753 56-57 21.00129344076983 28.6721761688067 29.932012840207527 20.394517550215934 58-59 21.39349221761688 28.080798382655576 30.01697414567694 20.50873525405061 60-61 20.770955914321323 28.05706718645836 31.11494956667923 20.057027332541086 62-63 21.18498376858642 27.712783687429347 30.96613141648068 20.13610112750355 64-65 20.805103475261586 28.31711399669575 30.824378278890467 20.053404249152198 66-67 20.681646908785254 28.212316309672183 30.522666009680876 20.583370771861688 68-69 20.581868246333887 28.595962066248198 30.223725804552416 20.598443882865503 70-71 20.93390408973653 28.79717254572331 29.76435465638677 20.504568708153386 72-73 21.054552766586475 28.248365989391612 29.890166227065883 20.80691501695603 74-75 20.68409249007275 29.202142691516187 29.490540129271615 20.623224689139448 76-77 20.95763528593374 28.258510622880493 30.0286585896061 20.755195501579664 78-79 20.819233500478244 28.43993652357903 30.019238572794993 20.721591403147734 80-81 20.645053766557492 29.02950276803571 29.7914372047187 20.534006260688095 82-83 20.544448631179257 29.190156664396167 29.399573919218565 20.86582078520601 84-85 20.471454580038586 28.83780332056194 29.40554151608199 21.285200583317483 86-87 20.694237123561635 28.96637053998435 29.22388119184951 21.115511144604504 88-89 20.820863888003245 28.574715225645637 29.071983420770415 21.53243746558071 90-91 20.583628719808754 28.308547441347702 29.514159074788864 21.593664764054672 92-93 20.741388212063296 28.795300767203198 29.398284435829385 21.065026584904125 94-95 20.408556998347873 28.693099475377526 29.268898002956433 21.629445523318164 96-97 20.08410988087302 28.67915060433031 29.250692008927277 21.986047505869394 98-99 20.507367213973023 28.8636999745474 28.598213970306507 22.030718841173066 100-101 20.466087952267838 28.842951697097185 28.96507239365625 21.725887956978728 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 842.0 1 556.5 2 395.0 3 511.5 4 443.5 5 428.5 6 713.5 7 1467.5 8 2283.0 9 2602.5 10 2320.5 11 1936.0 12 1949.5 13 2067.5 14 2127.5 15 2222.5 16 2238.5 17 2246.5 18 2292.5 19 2386.5 20 2596.0 21 2836.5 22 3150.0 23 3534.5 24 4015.0 25 4530.0 26 5277.5 27 6165.5 28 6661.5 29 7383.5 30 8410.0 31 9156.5 32 9973.0 33 10942.5 34 12068.5 35 12891.5 36 13832.0 37 15022.0 38 15375.0 39 15231.0 40 15491.5 41 16185.0 42 16526.0 43 17007.0 44 17971.5 45 18519.0 46 18688.5 47 20790.0 48 29515.0 49 28815.0 50 20049.5 51 16945.0 52 14427.0 53 13286.0 54 12597.5 55 11575.0 56 10155.0 57 9097.0 58 8337.0 59 7469.5 60 6529.0 61 5384.0 62 4179.0 63 3104.0 64 2252.5 65 1703.0 66 1235.0 67 890.0 68 648.0 69 427.5 70 303.0 71 228.5 72 204.5 73 197.0 74 157.0 75 141.0 76 112.0 77 66.0 78 46.0 79 31.0 80 21.5 81 18.0 82 11.5 83 8.0 84 6.0 85 1.0 86 1.5 87 1.5 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.022644271180545492 2 0.0 3 0.0 4 0.0 5 0.022825425349989856 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.003170197965276369 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0014492333555549115 24-25 0.0 26-27 7.246166777774558E-4 28-29 0.0015398104402770934 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 9.057708472218197E-5 40-41 4.5288542361090984E-4 42-43 0.014764064809715661 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 1.8115416944436394E-4 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 0.0014492333555549115 84-85 1.8115416944436394E-4 86-87 0.0 88-89 0.0 90-91 0.010054056404162199 92-93 0.0019926958638880033 94-95 0.0 96-97 0.0 98-99 0.0017209646097214573 100-101 0.018658879452769485 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 552016.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 57.58251055093317 #Duplication Level Percentage of deduplicated Percentage of total 1 79.89475063385744 46.00540321338271 2 11.83157201616768 13.625832409102024 3 3.656867811139141 6.3171488795486255 4 1.5467096303597938 3.5625369443769115 5 0.8159251391923852 2.349150896815857 6 0.49605420384690674 1.713842785610956 7 0.3281347011074545 1.3226373922053114 8 0.2254691121244382 1.0386462022251999 9 0.17431019208376547 0.9033496627318752 >10 0.928919420496902 9.508591915120785 >50 0.05937522073400217 2.360737723925874 >100 0.033021511852769504 3.6592164956887143 >500 0.005397747129779615 1.9897698172569307 >1k 0.002857630833412737 3.1856794354391953 >5k 6.350290740917194E-4 2.4574562265689983 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7284 1.319526970232747 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6157 1.1153662212689488 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4282 0.7757021535607663 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 2984 0.540564041621982 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 2131 0.3860395350859395 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 2043 0.37009796817483553 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1491 0.27010086664154664 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 1180 0.21376191994434945 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1166 0.21122576157212836 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1133 0.20524767398046434 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 1014 0.18369032781658504 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 948 0.171734152633257 No Hit CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA 773 0.14003217298049334 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 744 0.13477870206660678 No Hit GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT 725 0.13133677284716386 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 707 0.1280759977971653 No Hit TGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC 687 0.12445291440827803 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 653 0.11829367264716965 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 631 0.11430828091939364 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 609 0.11032288919161763 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 592 0.10724326831106346 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 592 0.10724326831106346 No Hit CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG 588 0.106518651633286 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 554 0.10035940987217762 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 1.8115416944436394E-4 0.0 0.0 0.0 0.0 6 1.8115416944436394E-4 0.0 0.0 0.0 0.0 7 1.8115416944436394E-4 0.0 0.0 0.0 0.0 8 1.8115416944436394E-4 0.0 0.0 0.0 0.0 9 1.8115416944436394E-4 0.0 0.0 0.0 0.0 10-11 1.8115416944436394E-4 0.0 0.0 0.0 0.0 12-13 1.8115416944436394E-4 0.0 0.0 0.0 0.0 14-15 1.8115416944436394E-4 0.0 0.0 0.0 0.0 16-17 2.7173125416654593E-4 0.0 0.0 0.0 0.0 18-19 3.623083388887279E-4 0.0 0.0 0.0 0.0 20-21 3.623083388887279E-4 0.0 0.0 0.0 0.0 22-23 3.623083388887279E-4 0.0 0.0 2.7173125416654593E-4 0.0 24-25 3.623083388887279E-4 0.0 0.0 5.434625083330917E-4 0.0 26-27 3.623083388887279E-4 0.0 0.0 6.340395930552738E-4 0.0 28-29 5.434625083330917E-4 0.0 0.0 0.0029890437958320047 0.0 30-31 5.434625083330917E-4 0.0 0.0 0.007789629286107649 0.0 32-33 5.434625083330917E-4 0.0 0.0 0.014673487724993479 0.0 34-35 5.434625083330917E-4 0.0 0.0 0.0221008086722124 0.0 36-37 5.434625083330917E-4 0.0 0.0 0.03007159212776441 0.0 38-39 5.434625083330917E-4 0.0 0.0 0.037589490159705516 0.0 40-41 5.434625083330917E-4 0.0 0.0 0.046103736123590616 0.0 42-43 5.434625083330917E-4 0.0 0.0 0.056248369612475 0.0 44-45 5.434625083330917E-4 0.0 0.0 0.0633133822208052 0.0 46-47 9.057708472218196E-4 0.0 0.0 0.07680936784441031 0.0 48-49 0.0010869250166661835 0.0 0.0 0.09021477638329324 0.0 50-51 0.0010869250166661835 0.0 0.0 0.10072171821106635 0.0 52-53 0.0010869250166661835 0.0 0.0 0.12336598939161184 0.0 54-55 0.0010869250166661835 0.0 0.0 0.16657125880409263 0.0 56-57 0.0010869250166661835 0.0 0.0 0.2515325642734993 0.0 58-59 0.0010869250166661835 0.0 0.0 0.3365844468276282 0.0 60-61 0.0010869250166661835 0.0 0.0 0.42217979189009014 0.0 62-63 0.0010869250166661835 0.0 0.0 0.5183726558650474 0.0 64-65 0.0010869250166661835 0.0 0.0 0.6267934262774992 0.0 66-67 0.0010869250166661835 0.0 0.0 0.7920966058954813 0.0 68-69 0.0010869250166661835 0.0 0.0 0.9841200255065071 0.0 70-71 0.0010869250166661835 0.0 0.0 1.190182893249471 0.0 72-73 0.0011775021013883656 0.0 0.0 1.3969703776702125 0.0 74-75 0.0012680791861105476 0.0 0.0 1.6141742268340047 0.0 76-77 0.0012680791861105476 0.0 0.0 1.860181588939451 0.0 78-79 0.0012680791861105476 0.0 0.0 2.0644329149879717 0.0 80-81 0.0014492333555549115 0.0 0.0 2.2291020550128984 0.0 82-83 0.0014492333555549115 0.0 0.0 2.4304549143503085 0.0 84-85 0.0014492333555549115 0.0 0.0 2.7418589316251705 0.0 86-87 0.0014492333555549115 0.0 0.0 3.117300947798615 0.0 88-89 0.0014492333555549115 0.0 0.0 3.5817802382539634 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTCTTAC 85 3.621917E-7 39.137165 1 GTGTGCG 60 0.0039500548 31.665247 9 GTACTAC 65 0.0058324155 29.240055 3 GTCGGGA 90 8.9250645E-4 26.397272 2 TGCGTAA 70 1.7800156E-5 23.748938 60-61 GAGTACT 5295 0.0 23.591955 12-13 TACCTGG 445 0.0 22.422852 2 GTACTTT 5655 0.0 22.006088 14-15 GTACATG 5155 0.0 21.756725 1 TACATGG 5265 0.0 21.027569 2 AGTACTT 5405 0.0 20.870943 12-13 AGGACCG 160 4.9710823E-5 20.787851 5 TTGCGTA 80 4.9907787E-5 20.78032 60-61 GTACCTG 505 0.0 20.703394 1 CATGGGG 1085 0.0 20.582573 4 ACATGGG 5235 0.0 20.421959 3 TTGCGCG 70 4.8500724E-4 20.358076 18-19 AGATAGC 140 4.848809E-4 20.356232 6 ACTTTTT 6415 0.0 19.659012 16-17 ACGCTTT 110 1.3452282E-6 19.430948 78-79 >>END_MODULE