FastQCFastQC Report
Thu 26 May 2016
SRR937697_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937697_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences515451
Sequences flagged as poor quality0
Sequence length101
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT54721.061594603560765No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT42760.8295647888936096No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT28340.5498097782330424No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA19370.37578741723267584No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT19190.3722953297209628No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA17590.3412545518390691No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT14380.2789789912135198No Hit
GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT12080.2343578730082976No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10440.20254107567935653No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT9680.187796706185457No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC9020.17499238530917585No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7860.1524878213448029No Hit
CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA7470.1449216317360913No Hit
CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG7260.14084752963909275No Hit
GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT7070.13716143726561789No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN7020.1361914129568087No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA6810.13211731085981016No Hit
TGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC5970.11582090247181595No Hit
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT5370.1041806107661058No Hit
ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC5310.10301658159553477No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT5290.1026285718720111No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGCGA250.001616443737.9966280-81
TTACGCG250.001616443737.9966280-81
GCCGTTC753.0741983E-431.6700068
GTTACGC300.00395060331.66692578-79
GGACCGA804.4874556E-429.690636
GTAGTAT1004.9279384E-528.5168691
GGCCGTT1004.9443046E-528.5030067
ATTGTAC1852.8740033E-1028.2517173
TACCTGG3650.027.3396222
GAGTACT36400.024.85940612-13
GTACATG48850.024.8099671
TACATGG48900.024.4882532
TGGCCGT1000.001651393823.7525046
CATGGGG11000.022.8932274
ACATGGG49950.022.7345453
AGTACTT37500.022.5468512-13
GTACTTT40650.022.26032814-15
GTATAGA1604.959752E-520.7935491
GTACCTG5050.020.7053151
CATGGGA38150.020.3009764
Produced by FastQC (version 0.11.5)