FastQCFastQC Report
Thu 26 May 2016
SRR937696_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937696_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences423444
Sequences flagged as poor quality0
Sequence length101
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT46391.0955403784207594No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT37710.890554595176694No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT24430.5769357931627322No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT15470.36533756529789063No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA14850.3506957236376002No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA14060.33203918345755284No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT12530.29590689677973947No Hit
GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT10770.2543429591634313No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9540.22529543457930684No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT8630.20380498956178386No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC7460.17617441739639716No Hit
CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG6950.16413032183712606No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6740.15917098837154384No Hit
GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT6260.1478353690216416No Hit
CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA6170.14570994039353494No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN5680.1341381623071764No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA5110.12067711432916749No Hit
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT5030.11878784443751712No Hit
TGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC4930.11642625707295416No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT4660.11004997118863416No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4260.1006036217303823No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGTAGG1553.6379788E-1133.7288741
GTACACG700.00836241227.1583161
GGACACG700.0083865927.1422486
GAGTACT33000.026.05244612-13
ACCATGC1858.936695E-925.6750988
TAGATAG1309.994081E-625.5793745
TACCTGG3500.024.4280222
GTACATG41450.023.9641841
GTCGGGA1000.00165213723.7494682
TACATGG41450.023.720822
AGTACTT34150.023.57560712-13
GTACTTT36200.023.42143614-15
GTATCAA77050.022.761171
ACATGGG43250.021.7451783
GTATAGC1754.0193336E-621.7266521
ACTGATC1100.002874762521.5904258
CATGGGG9250.021.0560724
GTGTAAG1604.9579176E-520.7930831
ACTTTTT41850.020.71339416-17
GTATAGA1150.003707187720.6639371
Produced by FastQC (version 0.11.5)