##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937696_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 423444 Sequences flagged as poor quality 0 Sequence length 101 %GC 41 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.950593230745977 33.0 31.0 34.0 31.0 34.0 2 32.02243507996335 33.0 31.0 34.0 30.0 34.0 3 32.04241647065491 34.0 31.0 34.0 30.0 34.0 4 35.64551865181701 37.0 35.0 37.0 35.0 37.0 5 35.6664446774544 37.0 35.0 37.0 35.0 37.0 6 35.76527002389926 37.0 35.0 37.0 35.0 37.0 7 35.70102540123369 37.0 35.0 37.0 35.0 37.0 8 35.72956518453444 37.0 35.0 37.0 35.0 37.0 9 37.28488773013669 39.0 37.0 39.0 34.0 39.0 10-11 37.221810912422896 39.0 37.0 39.0 34.0 39.0 12-13 37.1274324349855 39.0 37.0 39.0 34.0 39.0 14-15 38.19826234401715 40.0 38.0 41.0 33.5 41.0 16-17 38.1279189219826 40.0 38.0 41.0 33.0 41.0 18-19 38.16897393752185 40.0 38.0 41.0 33.5 41.0 20-21 38.237829559516726 40.0 38.0 41.0 34.0 41.0 22-23 38.23792520380499 40.0 38.0 41.0 34.0 41.0 24-25 38.158336167238176 40.0 38.0 41.0 34.0 41.0 26-27 38.02494308574451 40.0 38.0 41.0 33.5 41.0 28-29 37.866301092942635 40.0 38.0 41.0 33.0 41.0 30-31 37.561328062270334 40.0 37.0 41.0 33.0 41.0 32-33 37.33154915407941 39.5 36.0 41.0 33.0 41.0 34-35 37.24349500760431 40.0 36.0 41.0 33.0 41.0 36-37 37.042659714153466 40.0 36.0 41.0 32.0 41.0 38-39 36.81653772399656 39.0 35.5 41.0 31.0 41.0 40-41 36.54269631875762 39.0 35.0 41.0 30.5 41.0 42-43 36.1895162052125 39.0 35.0 41.0 30.0 41.0 44-45 35.777729758834695 39.0 35.0 41.0 29.0 41.0 46-47 35.171779503310944 39.0 35.0 40.0 25.5 41.0 48-49 34.623011543439034 39.0 35.0 40.0 23.0 41.0 50-51 33.67768937569076 37.5 33.5 39.5 19.5 40.5 52-53 33.75782039655775 38.0 33.5 39.5 20.0 40.5 54-55 34.00277368435968 38.0 34.0 40.0 18.5 41.0 56-57 33.76710615807521 38.0 34.0 40.0 15.5 41.0 58-59 33.49569010305967 38.0 34.0 40.0 11.5 41.0 60-61 33.12247192072624 38.0 33.0 40.0 8.0 41.0 62-63 32.750334164612084 37.0 33.0 40.0 6.0 41.0 64-65 32.298551874628046 36.5 32.0 39.5 2.0 41.0 66-67 31.757217010986103 36.0 31.0 39.0 2.0 40.5 68-69 31.312140448323746 35.5 31.0 39.0 2.0 40.0 70-71 30.818875695487478 35.0 30.5 38.0 2.0 40.0 72-73 30.314793691727832 35.0 29.5 37.5 2.0 40.0 74-75 29.773928783971435 34.5 29.0 37.0 2.0 39.0 76-77 29.215052049385516 34.0 29.0 36.5 2.0 39.0 78-79 28.699437233731025 34.0 28.0 36.0 2.0 38.5 80-81 28.13666506078726 34.0 26.5 35.5 2.0 37.5 82-83 27.58422129018241 34.0 26.0 35.0 2.0 37.0 84-85 27.036843124474547 33.0 25.5 35.0 2.0 36.5 86-87 26.438551496774075 33.0 24.5 35.0 2.0 36.0 88-89 25.845265253492787 32.0 22.0 35.0 2.0 35.5 90-91 25.237155326324142 32.0 19.5 34.5 2.0 35.0 92-93 24.570028150121388 31.5 14.5 34.0 2.0 35.0 94-95 23.79394441767979 31.0 4.5 34.0 2.0 35.0 96-97 22.90748835737429 31.0 2.0 34.0 2.0 35.0 98-99 21.721596007972718 30.0 2.0 34.0 2.0 35.0 100-101 18.65604778908191 24.5 2.0 32.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.35196819298156257 1101 2 0.32079393364574926 1101 3 0.18716257952504733 1101 4 0.22921909460455936 1101 5 0.12385129064423239 1101 6 0.1647969572047785 1101 7 0.053286270786649936 1101 8 0.2486477681880217 1101 9 0.22233010838521494 1101 10-11 0.33522987414018957 1101 12-13 0.32155700424215894 1101 14-15 0.4596601824419011 1101 16-17 0.3642587817437928 1101 18-19 0.4447758989674213 1101 20-21 0.5162387383386076 1101 22-23 0.29566362429348914 1101 24-25 0.18595856377480402 1101 26-27 0.2507246693915377 1101 28-29 0.2924230545512856 1101 30-31 0.36146782063302396 1101 32-33 0.3056476822379963 1101 34-35 0.5421734774831393 1101 36-37 0.8431934408830131 1101 38-39 0.8769466852050911 1101 40-41 0.7821341504716699 1101 42-43 0.6423640378539446 1101 44-45 0.8125400066635677 1101 46-47 1.0920899024788824 1101 48-49 1.2765996472548693 1101 50-51 1.0385642894438334 1101 52-53 1.0336041947507724 1101 54-55 1.1120957529706104 1101 56-57 1.3029161380900405 1101 58-59 1.1945166728719627 1101 60-61 1.216524503742768 1101 62-63 1.1475637311377582 1101 64-65 1.2327794136553862 1101 66-67 1.3042408501279539 1101 68-69 1.3893755727783912 1101 70-71 1.190509258004841 1101 72-73 1.2226575275320108 1101 74-75 1.2194384929098483 1101 76-77 1.3396353089275124 1101 78-79 1.6052392969090086 1101 80-81 1.5700386811534557 1101 82-83 1.8232044392335354 1101 84-85 2.0730097270914634 1101 86-87 2.396378032748533 1101 88-89 2.2115040616786352 1101 90-91 2.3785575531339234 1101 92-93 2.123665060409234 1101 94-95 2.4342274697206285 1101 96-97 2.584998313997019 1101 98-99 3.3753453389287493 1101 100-101 4.222450333674551 1102 1 -0.024932973989294283 1102 2 -0.03217326949567223 1102 3 -0.1746675183677695 1102 4 -0.0977401443755852 1102 5 -0.2604333175079745 1102 6 -0.06408971941709041 1102 7 -0.004080578392049006 1102 8 -0.12004035746873853 1102 9 0.0499530274715454 1102 10-11 0.09221234192404637 1102 12-13 0.1588171115371111 1102 14-15 0.1632924483564011 1102 16-17 0.4672605515574091 1102 18-19 0.05418632980642002 1102 20-21 0.15000484640785317 1102 22-23 -0.038333389146025354 1102 24-25 0.234317338178883 1102 26-27 0.022930299611097382 1102 28-29 -0.009171160370463838 1102 30-31 0.21572781731462953 1102 32-33 0.16312237943395047 1102 34-35 0.22198930625367552 1102 36-37 0.33110201894838553 1102 38-39 0.23814075632947151 1102 40-41 0.30493432745303295 1102 42-43 0.3124735450714624 1102 44-45 0.24503129586217653 1102 46-47 0.2943395015054975 1102 48-49 0.1870927062670944 1102 50-51 0.07994750939847961 1102 52-53 0.33507396965260483 1102 54-55 0.12471950165707568 1102 56-57 0.2614223047843609 1102 58-59 0.0951858836456978 1102 60-61 0.06296551917332494 1102 62-63 0.4181378144296062 1102 64-65 0.34261847122963474 1102 66-67 0.3742245346586728 1102 68-69 0.052509525546490465 1102 70-71 0.6174214863107998 1102 72-73 0.6107444696259208 1102 74-75 0.5286995957249623 1102 76-77 0.31075831097385986 1102 78-79 0.71541019453635 1102 80-81 0.9870095345354564 1102 82-83 0.9672921556209033 1102 84-85 1.0122829973751841 1102 86-87 1.2754154200611794 1102 88-89 1.4845116386931814 1102 90-91 1.117406344683065 1102 92-93 1.1185699329273469 1102 94-95 1.9504046723089807 1102 96-97 2.0647491574238224 1102 98-99 1.8523101085764466 1102 100-101 2.5249294554045427 1103 1 0.2705318786184172 1103 2 0.18664759218232874 1103 3 0.30886989659821396 1103 4 0.3786770891845066 1103 5 0.3869136212675386 1103 6 0.2909216184513923 1103 7 0.3459420973449099 1103 8 0.2727941096514428 1103 9 0.3547149322334491 1103 10-11 0.5467474893163384 1103 12-13 0.6394973836459528 1103 14-15 0.6075328111681841 1103 16-17 0.7866029551855291 1103 18-19 0.7069981211896348 1103 20-21 0.6535422613738433 1103 22-23 0.5427096947542012 1103 24-25 0.4947255014441865 1103 26-27 0.7492341544863805 1103 28-29 0.5741168214889498 1103 30-31 0.8277686336411563 1103 32-33 0.7076802025632034 1103 34-35 0.8261843175915402 1103 36-37 0.8522584815334184 1103 38-39 0.9577325930641649 1103 40-41 0.9094241233714015 1103 42-43 1.011090325116811 1103 44-45 1.1866185974494812 1103 46-47 0.940938140961272 1103 48-49 1.0313557448158477 1103 50-51 1.117453178332724 1103 52-53 1.1546204549133776 1103 54-55 1.2414995470085515 1103 56-57 1.138905297981637 1103 58-59 1.0905600333055645 1103 60-61 1.3251423899216306 1103 62-63 1.2924688801892472 1103 64-65 1.2788769746309896 1103 66-67 1.2671270289897407 1103 68-69 1.0015571445941163 1103 70-71 1.3036799897121618 1103 72-73 1.2146900478572142 1103 74-75 1.4693571920968402 1103 76-77 1.4311664742391663 1103 78-79 1.7233693782098207 1103 80-81 1.945512935895998 1103 82-83 1.8417139243283884 1103 84-85 2.0977251742459337 1103 86-87 2.236662585594061 1103 88-89 2.195758804226063 1103 90-91 2.046159179150184 1103 92-93 2.7586379601382305 1103 94-95 2.699701724463175 1103 96-97 2.5238194522084 1103 98-99 2.9100878863542228 1103 100-101 3.6972877320485367 1104 1 -0.11161543723676459 1104 2 -0.2519291868438849 1104 3 -0.2876844105178904 1104 4 -0.22594213678303277 1104 5 -0.2686169654990067 1104 6 -0.43212457380828084 1104 7 -0.09820272163136679 1104 8 -0.2106515832324547 1104 9 0.004402822370778381 1104 10-11 -0.192266243517615 1104 12-13 0.08696305156105666 1104 14-15 -0.03030738858212345 1104 16-17 -0.013933873778270822 1104 18-19 -0.05653496245580669 1104 20-21 -0.08261254511804594 1104 22-23 -0.008401416356910829 1104 24-25 -0.20157911778177606 1104 26-27 -0.225547368609746 1104 28-29 -0.041401280883086145 1104 30-31 -0.04814896935509694 1104 32-33 -0.30711380492743245 1104 34-35 -0.5883600020089546 1104 36-37 -0.424183466032126 1104 38-39 -0.06940848059375071 1104 40-41 -0.029627062646078173 1104 42-43 -0.4468247810005437 1104 44-45 -0.35662734512230543 1104 46-47 -0.7425200363171243 1104 48-49 -0.3781573637983797 1104 50-51 -0.29046192778463364 1104 52-53 -0.438413252951797 1104 54-55 -0.5669898412561167 1104 56-57 -0.44744925882235975 1104 58-59 -0.2894149979054177 1104 60-61 -0.5495392580184486 1104 62-63 -0.3876559637558259 1104 64-65 -0.5324713399757499 1104 66-67 -0.2878916976020207 1104 68-69 -0.2989796843696837 1104 70-71 -0.5359454784526356 1104 72-73 -0.4601414128169452 1104 74-75 -0.5108300738207596 1104 76-77 -0.47307821989317134 1104 78-79 -0.3023609588688281 1104 80-81 -0.15386284437865783 1104 82-83 -0.07853576356175296 1104 84-85 -0.3212261366154898 1104 86-87 -0.0785808600988247 1104 88-89 -0.04638851162912161 1104 90-91 0.14031648252097284 1104 92-93 0.20037329097468515 1104 94-95 0.6161311876342133 1104 96-97 0.23561720501738748 1104 98-99 0.7816484356675844 1104 100-101 1.8685611402882358 1105 1 0.442076594065572 1105 2 0.3451841775481874 1105 3 0.18648507274997783 1105 4 0.25970689948260883 1105 5 0.036452916660493884 1105 6 0.06588096804488686 1105 7 0.2965112030359691 1105 8 0.3265610473208156 1105 9 0.5265306503906331 1105 10-11 0.4802163240046866 1105 12-13 0.5031288199603168 1105 14-15 0.689673732577404 1105 16-17 0.5211016001719742 1105 18-19 0.6974859260676922 1105 20-21 0.6981493074442966 1105 22-23 0.7780484481417318 1105 24-25 0.6788447426365991 1105 26-27 0.6890715528603693 1105 28-29 0.6199975802965341 1105 30-31 0.6592320482752996 1105 32-33 0.5671652974141494 1105 34-35 0.8124986807351746 1105 36-37 0.8821500804494065 1105 38-39 0.8803342190804315 1105 40-41 1.0199390285204544 1105 42-43 0.9790849050626136 1105 44-45 1.6265643969074688 1105 46-47 1.2082823144030073 1105 48-49 1.2091877231356278 1105 50-51 0.9579409832107757 1105 52-53 1.2852979616884355 1105 54-55 1.6466485984990697 1105 56-57 1.7033226421550793 1105 58-59 1.5278500062052913 1105 60-61 1.3411857503552298 1105 62-63 1.5723604791052352 1105 64-65 1.4590666765280105 1105 66-67 1.7212462701821494 1105 68-69 1.4506899359220284 1105 70-71 1.1010783636959012 1105 72-73 1.3188634895916316 1105 74-75 1.599519793722859 1105 76-77 1.913822300797431 1105 78-79 1.800361248128521 1105 80-81 1.864754128307922 1105 82-83 1.6938006451955943 1105 84-85 2.0445544425386153 1105 86-87 2.259182910797314 1105 88-89 2.176951216150183 1105 90-91 2.343665954217933 1105 92-93 2.789992973688367 1105 94-95 2.7255553829997616 1105 96-97 2.786556579579674 1105 98-99 3.2249388348637105 1105 100-101 3.3122199813710296 1106 1 -0.4116002535137149 1106 2 -0.3279920474573075 1106 3 -0.11266163493331405 1106 4 -0.1811577456503244 1106 5 0.08927097862489575 1106 6 -0.09580510827533573 1106 7 -0.27584468944187535 1106 8 -0.14986354800621626 1106 9 0.039925142443664186 1106 10-11 -0.4698816398127903 1106 12-13 -0.7251497635012001 1106 14-15 -1.0402299515945757 1106 16-17 -0.7564976454150667 1106 18-19 -0.288895272203753 1106 20-21 -0.21685563652405904 1106 22-23 -0.21346898392447855 1106 24-25 -0.7338255496068626 1106 26-27 -0.42426434401210855 1106 28-29 -0.16402882415669495 1106 30-31 -0.5662403753678547 1106 32-33 -0.28578826590526063 1106 34-35 -0.1945739406667144 1106 36-37 -0.17920548243053958 1106 38-39 -0.5763740135424342 1106 40-41 -0.5079257264784474 1106 42-43 -0.16791117638469188 1106 44-45 -0.12361413528325471 1106 46-47 0.30858078360391517 1106 48-49 -0.06015326419316125 1106 50-51 -0.04871048533094324 1106 52-53 0.14779613058905028 1106 54-55 -0.41578273527373 1106 56-57 -0.4822959032195655 1106 58-59 -0.9957162429707154 1106 60-61 -0.6761639163846738 1106 62-63 -0.1960146032942376 1106 64-65 0.14090400165802208 1106 66-67 0.4485909929537044 1106 68-69 0.46791600345297013 1106 70-71 0.1955493590815287 1106 72-73 -0.035445087277924614 1106 74-75 -0.027759925020273357 1106 76-77 0.2136739817466733 1106 78-79 -0.08426575692531557 1106 80-81 0.07509251547558193 1106 82-83 0.26420641682087975 1106 84-85 0.3080404895612503 1106 86-87 0.5977211441526187 1106 88-89 0.5232212666885268 1106 90-91 0.6729159359069428 1106 92-93 0.9759502724505431 1106 94-95 0.9398443671058168 1106 96-97 0.8576032359921832 1106 98-99 0.7428881866545218 1106 100-101 0.880643587904391 1107 1 0.2132219370979449 1107 2 0.4772177676209246 1107 3 0.2587821772999668 1107 4 0.45886714766403713 1107 5 0.34887268559501905 1107 6 0.44269062429934536 1107 7 0.4919362493916992 1107 8 0.4343291973707366 1107 9 0.535124288958599 1107 10-11 0.5771933957490916 1107 12-13 0.6486055707804539 1107 14-15 1.1088632205249098 1107 16-17 1.0215517855948804 1107 18-19 0.9519030919498803 1107 20-21 0.850267407572673 1107 22-23 0.7685722678536067 1107 24-25 0.7128760862395112 1107 26-27 0.7515002363577281 1107 28-29 0.9529253010211178 1107 30-31 0.8248958266236102 1107 32-33 0.8131626587035541 1107 34-35 0.7170103409833573 1107 36-37 0.5259865517088613 1107 38-39 0.8604592012513024 1107 40-41 0.601427047348011 1107 42-43 0.241850559034944 1107 44-45 0.41248117054888667 1107 46-47 0.48647907663379186 1107 48-49 0.10729872727199563 1107 50-51 -0.2843523772228309 1107 52-53 -0.251563755612942 1107 54-55 0.01744983940621836 1107 56-57 0.04419769564245257 1107 58-59 -0.073241136285084 1107 60-61 0.40207221448303443 1107 62-63 0.345948412352989 1107 64-65 0.08756118515730549 1107 66-67 0.14635948513009112 1107 68-69 0.097361238161362 1107 70-71 0.07179402479988184 1107 72-73 0.31186110048879456 1107 74-75 0.3168791219214029 1107 76-77 0.345786357280101 1107 78-79 0.21205358873613633 1107 80-81 0.6504836961299176 1107 82-83 0.595909830761137 1107 84-85 0.4274839461757587 1107 86-87 1.046289778576515 1107 88-89 0.9975278100740148 1107 90-91 1.1645144423080787 1107 92-93 1.2603119367464153 1107 94-95 1.6736637127672687 1107 96-97 2.078753662734716 1107 98-99 2.334948997465336 1107 100-101 2.781914925030989 1108 1 0.24241469390959125 1108 2 0.40261758146421656 1108 3 0.2980231656228689 1108 4 0.4283734092988354 1108 5 0.4866921135862228 1108 6 0.4671131154145911 1108 7 0.45489529491547387 1108 8 0.44415531008016274 1108 9 0.7742933530980451 1108 10-11 0.6565902903167 1108 12-13 0.640133325053597 1108 14-15 0.5457285953768292 1108 16-17 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-2.2287600991831766 2316 84-85 -1.8555398997017356 2316 86-87 -2.0571826931317645 2316 88-89 -2.0708775249358666 2316 90-91 -1.9396428686313847 2316 92-93 -1.5380590440331794 2316 94-95 -1.7038174260866903 2316 96-97 -2.4193546092250457 2316 98-99 -2.8526576578247678 2316 100-101 -2.689731608110737 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1559.0 3 266.0 4 242.0 5 259.0 6 313.0 7 370.0 8 435.0 9 588.0 10 809.0 11 1148.0 12 1888.0 13 2520.0 14 2885.0 15 3877.0 16 5568.0 17 8103.0 18 6658.0 19 3744.0 20 3280.0 21 3305.0 22 3654.0 23 3959.0 24 4265.0 25 4907.0 26 5655.0 27 6602.0 28 7634.0 29 9196.0 30 11317.0 31 13918.0 32 17634.0 33 22660.0 34 31213.0 35 43417.0 36 58883.0 37 75607.0 38 50695.0 39 4411.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 46.812396512148496 18.69994385895094 14.586249943740508 19.901409685160047 2 14.63566515526406 21.113781387254495 41.73531117924919 22.51524227823225 3 20.121282727038093 27.276063828527725 30.603169641905765 21.99948380252842 4 11.59237388063589 16.686161321027235 38.84329612774602 32.87816867059085 5 11.94170504457362 39.48547953638699 33.83214746587424 14.74066795316514 6 28.49211983529746 36.85124710114061 18.61044062662691 16.04619243693502 7 26.368256912862208 35.91139042823543 20.535566398023747 17.184786260878617 8 26.102430493931454 33.278197018852836 20.32471266407786 20.29465982313785 9 27.521007906213985 16.90145511253517 21.411381067208733 34.16615591404211 10-11 24.436684963632857 27.339075243956263 26.63811882418229 21.586120968228595 12-13 26.855949450965543 25.558145588792126 27.63075066262779 19.95515429761454 14-15 23.338164908564902 25.620995462263423 28.775757719180962 22.265081909990712 16-17 19.853881602688105 29.611869309812413 29.131985731272298 21.402263356227188 18-19 19.58486391841612 27.447131964531835 33.456608282609594 19.511395834442453 20-21 21.23774663483493 25.725027742352218 33.09668535085499 19.940540271957865 22-23 20.42438906077152 26.01468265470916 33.092171814272106 20.468756470247214 24-25 20.321127673746968 26.018771688422625 33.63984529270275 20.020255345127655 26-27 20.305942680411885 25.76423553965908 34.03786836348234 19.891953416446697 28-29 19.48001239981353 26.556425067500893 34.1198380451836 19.843724487501984 30-31 19.534085167660002 26.61841390645023 33.8582211320585 19.989279793831265 32-33 19.40186018005483 26.616239956364684 33.613862967603616 20.368036895976864 34-35 20.156181596601865 26.092147141995103 33.50320053953643 20.248470721866607 36-37 20.295211683530045 25.95477648528674 33.86652532346454 19.883486507718665 38-39 20.251437954425537 26.189935383734493 33.79340776862124 19.765218893218723 40-41 19.862038125838456 26.617579905974875 33.79450755111167 19.725874417075 42-43 19.587999649804434 26.996173876830543 32.86407916312724 20.55174731023778 44-45 19.810542828985742 27.18439480536689 32.29183550091865 20.713226864728714 46-47 20.27124234816415 27.434922422748105 31.494684236804062 20.799150992283685 48-49 20.594224350396193 27.406116409854125 31.10584983594997 20.89380940379971 50-51 20.52145435529269 27.9295278231641 31.070392906876727 20.47862491466648 52-53 20.50047269100207 28.375895243628136 30.66646473275506 20.45716733261473 54-55 19.82614909720341 27.95031276361885 31.01595230517494 21.207585834002796 56-57 20.535156619664996 28.46432289202233 30.0820952020441 20.918425286268572 58-59 20.754226021791276 27.768854467779096 30.335101173776664 21.14181833665296 60-61 20.43823423990385 27.57826076155709 31.31140229988395 20.67210269865511 62-63 20.711656383663275 27.58738975737159 31.094572088269807 20.606381770695332 64-65 20.318447980126574 28.086946235287158 31.1060507482108 20.488555036375466 66-67 20.230604618480307 28.07605906714789 30.720429458811395 20.972906855560407 68-69 20.04296323284132 28.199471476529915 30.76011327530773 20.99745201532103 70-71 20.257081816181532 28.394457102163166 30.092129264882285 21.256331816773017 72-73 20.2917925651103 27.960102122665504 30.358541415757085 21.389563896467116 74-75 20.07347243009175 28.616032607088137 30.203321048490622 21.10717391432949 76-77 20.277477409264392 27.948981257793477 30.32458728577547 21.448954047166662 78-79 20.21086803238736 28.05039348466995 30.539899771433436 21.19883871150925 80-81 20.113335896601697 28.472508946792463 30.167223684599687 21.246931472006153 82-83 20.039840496066226 28.460995231679348 30.10110353915618 21.398060733098248 84-85 20.031653954615138 28.136769687908092 29.921030717111112 21.910545640365655 86-87 20.25513662372198 28.246609988040472 29.940935776973117 21.557317611264434 88-89 20.41596623246647 27.807937689058082 29.735753434542872 22.040342643932572 90-91 19.933705343675804 27.669264902989056 30.311468951884322 22.085560801450814 92-93 20.118956363739603 27.95699306324466 30.224214291797974 21.699836281217767 94-95 19.72444288820383 27.89841271343433 30.192277208514422 22.184867189847424 96-97 19.513132895294113 27.785165187475897 30.061092037923437 22.64060987930655 98-99 19.82486939733495 28.0451478270745 29.39222440944882 22.737758366141733 100-101 20.177518922301168 27.308319661805637 30.388642006266625 22.125519409626566 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 705.0 1 451.0 2 257.5 3 304.5 4 277.0 5 275.5 6 431.5 7 904.5 8 1531.0 9 1818.0 10 1541.0 11 1274.5 12 1284.0 13 1328.5 14 1372.5 15 1418.0 16 1475.0 17 1523.0 18 1552.5 19 1637.5 20 1782.5 21 1981.0 22 2258.5 23 2637.0 24 3004.0 25 3483.0 26 4152.0 27 4852.0 28 5420.5 29 5993.0 30 6681.5 31 7321.5 32 8146.5 33 8991.5 34 9798.0 35 10394.5 36 11222.5 37 12136.0 38 12337.0 39 12149.0 40 12070.5 41 12380.0 42 12632.0 43 13133.0 44 13986.5 45 14394.5 46 14676.0 47 16285.0 48 21832.0 49 21732.5 50 15719.5 51 12870.5 52 11161.5 53 10147.5 54 9453.0 55 8685.5 56 7676.5 57 6790.5 58 6122.0 59 5440.5 60 4734.5 61 3950.5 62 3130.0 63 2356.5 64 1692.5 65 1262.0 66 891.5 67 624.5 68 461.0 69 330.5 70 253.5 71 184.5 72 131.0 73 114.5 74 101.5 75 89.0 76 66.0 77 40.5 78 29.0 79 20.0 80 12.0 81 6.5 82 4.5 83 3.0 84 2.0 85 3.5 86 2.5 87 0.0 88 1.0 89 1.5 90 0.5 91 0.5 92 0.5 93 0.0 94 0.5 95 1.0 96 0.5 97 0.0 98 0.5 99 0.5 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.3053532462379913 2 0.2595384513654698 3 0.26567857851333354 4 0.2611915625206639 5 0.2619000387300328 6 0.20404114829824013 7 0.19506711631290088 8 0.20191571967013347 9 0.20427730703469643 10-11 0.20746544997685645 12-13 0.20876432302736608 14-15 0.20699313250394386 16-17 0.19872757672797348 18-19 0.1916428146342846 20-21 0.19010778284731863 22-23 0.197546783045692 24-25 0.19825525925506088 26-27 0.20038068788316754 28-29 0.20238803714304607 30-31 0.20722929124040015 32-33 0.20085300535608014 34-35 0.20286035461595867 36-37 0.19648406873163868 38-39 0.18774619548275567 40-41 0.1868015605369305 42-43 0.194358640103532 44-45 0.19258744958010976 46-47 0.19672022746809492 48-49 0.2029784339841868 50-51 0.1977829417821483 52-53 0.203923068930012 54-55 0.19849141799151718 56-57 0.1804252746526105 58-59 0.1667280679381453 60-61 0.18196030643957642 62-63 0.1747574649776594 64-65 0.18160606833489198 66-67 0.18243262391248902 68-69 0.17877216349741643 70-71 0.18349533822654235 72-73 0.1924693702118816 74-75 0.197546783045692 76-77 0.1924693702118816 78-79 0.19128857652960013 80-81 0.1904620209520031 82-83 0.17912640160210086 84-85 0.17664673486930974 86-87 0.18148798896666382 88-89 0.18644732243224604 90-91 0.18550268748642088 92-93 0.18136990959843569 94-95 0.18255070328071715 96-97 0.18963546537440606 98-99 0.1860930843275616 100-101 0.17204163950841198 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 423444.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 63.238484828521734 #Duplication Level Percentage of deduplicated Percentage of total 1 82.87206160695291 52.4070361063961 2 10.45853592033784 13.227639302536682 3 2.990679955230552 5.673782069274339 4 1.2335214906448593 3.1202412028720174 5 0.6402218950976862 2.024333130001123 6 0.4029606029552153 1.5289570785885211 7 0.27072211685146913 1.1984039535479842 8 0.1848367596904907 0.9351037298752171 9 0.12900110790834413 0.7342051144791883 >10 0.7457338723007815 8.356615350052877 >50 0.040180837216448065 1.7625344311336932 >100 0.024784441639163476 3.2419994674867127 >500 0.0037552184301762846 1.6046140054063667 >1k 0.003004174744141028 4.184535058349207 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4639 1.0955403784207594 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3771 0.890554595176694 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2443 0.5769357931627322 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 1547 0.36533756529789063 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 1485 0.3506957236376002 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 1406 0.33203918345755284 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 1253 0.29590689677973947 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 1077 0.2543429591634313 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 954 0.22529543457930684 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 863 0.20380498956178386 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 746 0.17617441739639716 No Hit CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG 695 0.16413032183712606 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 674 0.15917098837154384 No Hit GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT 626 0.1478353690216416 No Hit CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA 617 0.14570994039353494 No Hit NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 568 0.1341381623071764 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 511 0.12067711432916749 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 503 0.11878784443751712 No Hit TGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC 493 0.11642625707295416 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 466 0.11004997118863416 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 426 0.1006036217303823 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 2.3615873645629646E-4 0.0 0.0 0.0 0.0 8 2.3615873645629646E-4 0.0 0.0 0.0 0.0 9 2.3615873645629646E-4 0.0 0.0 0.0 0.0 10-11 2.3615873645629646E-4 0.0 0.0 0.0 0.0 12-13 2.3615873645629646E-4 0.0 0.0 0.0 0.0 14-15 2.3615873645629646E-4 0.0 0.0 0.0 0.0 16-17 2.3615873645629646E-4 0.0 0.0 0.0 0.0 18-19 2.3615873645629646E-4 0.0 0.0 0.0 0.0 20-21 2.3615873645629646E-4 0.0 0.0 1.1807936822814823E-4 0.0 22-23 2.3615873645629646E-4 0.0 0.0 5.903968411407411E-4 0.0 24-25 2.3615873645629646E-4 0.0 0.0 7.084762093688894E-4 0.0 26-27 2.3615873645629646E-4 0.0 0.0 0.0014169524187377788 0.0 28-29 3.542381046844447E-4 0.0 0.0 0.003424301678616299 0.0 30-31 4.723174729125929E-4 0.0 0.0 0.009800587562936303 0.0 32-33 4.723174729125929E-4 0.0 0.0 0.017003429024853346 0.0 34-35 4.723174729125929E-4 0.0 0.0 0.02467858795968298 0.0 36-37 4.723174729125929E-4 0.0 0.0 0.03483341362730373 0.0 38-39 4.723174729125929E-4 0.0 0.0 0.04156393761630818 0.0 40-41 4.723174729125929E-4 0.0 0.0 0.04770406476417188 0.0 42-43 4.723174729125929E-4 0.0 0.0 0.05644193801305486 0.0 44-45 4.723174729125929E-4 0.0 0.0 0.06388093821142819 0.0 46-47 4.723174729125929E-4 0.0 0.0 0.0771058274529808 0.0 48-49 7.084762093688894E-4 0.0 0.0 0.09162958974504304 0.0 50-51 7.084762093688894E-4 0.0 0.0 0.10261097099026081 0.0 52-53 7.084762093688894E-4 0.0 0.0 0.11902400317397342 0.0 54-55 7.084762093688894E-4 0.0 0.0 0.16165065510433493 0.0 56-57 7.084762093688894E-4 0.0 0.0 0.2500921019072179 0.0 58-59 7.084762093688894E-4 0.0 0.0 0.34550023143556174 0.0 60-61 7.084762093688894E-4 0.0 0.0 0.42815578919526553 0.0 62-63 7.084762093688894E-4 0.0 0.0 0.5315933157631234 0.0 64-65 7.084762093688894E-4 0.0 0.0 0.6521523507240627 0.0 66-67 7.084762093688894E-4 0.0 0.0 0.8304521967485665 0.0 68-69 7.084762093688894E-4 0.0 0.0 1.054566837645592 0.0 70-71 7.084762093688894E-4 0.0 0.0 1.2667554623515742 0.0 72-73 7.084762093688894E-4 0.0 0.0 1.499962214602167 0.0 74-75 7.084762093688894E-4 0.0 0.0 1.7388367765277108 0.0 76-77 7.084762093688894E-4 0.0 0.0 2.0134893870263837 0.0 78-79 7.084762093688894E-4 0.0 0.0 2.232172376984914 0.0 80-81 0.0014169524187377788 0.0 0.0 2.418383540680704 0.0 82-83 0.0014169524187377788 0.0 0.0 2.642143943473045 0.0 84-85 0.0014169524187377788 0.0 0.0 2.9923673496377328 0.0 86-87 0.0014169524187377788 0.0 0.0 3.3985603763425623 0.0 88-89 0.0014169524187377788 0.0 0.0 3.9269655491635262 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTGTAGG 155 3.6379788E-11 33.728874 1 GTACACG 70 0.008362412 27.158316 1 GGACACG 70 0.00838659 27.142248 6 GAGTACT 3300 0.0 26.052446 12-13 ACCATGC 185 8.936695E-9 25.675098 8 TAGATAG 130 9.994081E-6 25.579374 5 TACCTGG 350 0.0 24.428022 2 GTACATG 4145 0.0 23.964184 1 GTCGGGA 100 0.001652137 23.749468 2 TACATGG 4145 0.0 23.72082 2 AGTACTT 3415 0.0 23.575607 12-13 GTACTTT 3620 0.0 23.421436 14-15 GTATCAA 7705 0.0 22.76117 1 ACATGGG 4325 0.0 21.745178 3 GTATAGC 175 4.0193336E-6 21.726652 1 ACTGATC 110 0.0028747625 21.590425 8 CATGGGG 925 0.0 21.056072 4 GTGTAAG 160 4.9579176E-5 20.793083 1 ACTTTTT 4185 0.0 20.713394 16-17 GTATAGA 115 0.0037071877 20.663937 1 >>END_MODULE