##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937696_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 423444 Sequences flagged as poor quality 0 Sequence length 101 %GC 41 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.364187472251345 33.0 31.0 34.0 31.0 34.0 2 32.55526350591814 34.0 31.0 34.0 31.0 34.0 3 32.63960523705614 34.0 31.0 34.0 31.0 34.0 4 36.22738780098431 37.0 35.0 37.0 35.0 37.0 5 36.12642285638715 37.0 35.0 37.0 35.0 37.0 6 36.255896883649314 37.0 36.0 37.0 35.0 37.0 7 36.22391390597104 37.0 36.0 37.0 35.0 37.0 8 36.23646101963896 37.0 37.0 37.0 35.0 37.0 9 37.89707966106498 39.0 38.0 39.0 35.0 39.0 10-11 37.87300918185167 39.0 38.0 39.0 35.0 39.0 12-13 37.79595294773335 39.0 38.0 39.0 35.0 39.0 14-15 38.93248575962819 40.0 38.0 41.0 35.5 41.0 16-17 38.93527833668679 40.0 38.0 41.0 35.5 41.0 18-19 39.03537775951483 40.0 38.5 41.0 36.0 41.0 20-21 39.02234061646877 40.0 38.0 41.0 36.0 41.0 22-23 38.88493283645535 40.0 38.0 41.0 35.0 41.0 24-25 38.813470022009994 40.0 38.0 41.0 35.0 41.0 26-27 38.688419247881654 40.0 38.0 41.0 35.0 41.0 28-29 38.5575117370892 40.0 38.0 41.0 35.0 41.0 30-31 38.4943085744514 40.0 38.0 41.0 35.0 41.0 32-33 38.274499107319976 40.0 37.0 41.0 35.0 41.0 34-35 38.07424476436081 40.0 37.0 41.0 34.0 41.0 36-37 37.85461832025014 40.0 37.0 41.0 34.0 41.0 38-39 37.65884863169628 40.0 36.0 41.0 33.0 41.0 40-41 37.40836686787391 40.0 36.0 41.0 33.0 41.0 42-43 37.09895051057519 40.0 36.0 41.0 33.0 41.0 44-45 36.600863396340486 40.0 35.5 41.0 31.5 41.0 46-47 35.90707035641077 40.0 35.0 41.0 29.5 41.0 48-49 35.275812149894676 39.0 35.0 41.0 25.0 41.0 50-51 34.90509725016767 39.0 35.0 41.0 23.0 41.0 52-53 34.64543009229084 39.0 35.0 41.0 21.5 41.0 54-55 34.426965549163526 39.0 35.0 40.0 20.0 41.0 56-57 34.158585314705135 38.5 34.5 40.0 17.5 41.0 58-59 33.91471245312249 38.0 34.0 40.0 14.0 41.0 60-61 33.54092158585315 38.0 34.0 40.0 9.0 41.0 62-63 33.179782922889444 37.5 33.5 40.0 7.5 41.0 64-65 32.77405040572071 37.0 33.0 40.0 2.0 41.0 66-67 32.35242322479478 36.0 33.0 39.0 2.0 40.5 68-69 31.907981929133484 36.0 32.0 39.0 2.0 40.0 70-71 31.482897384305836 35.0 31.5 38.5 2.0 40.0 72-73 31.025526397823562 35.0 31.0 37.5 2.0 40.0 74-75 30.5475021490445 35.0 31.0 37.0 2.0 39.0 76-77 29.36032273452924 33.5 29.5 35.5 2.0 38.5 78-79 29.843250819470818 34.0 30.0 36.0 2.0 38.5 80-81 29.621184855612547 34.0 30.0 36.0 2.0 37.5 82-83 29.283292477871925 34.0 30.0 35.0 2.0 37.0 84-85 28.893102747943058 34.0 29.5 35.0 2.0 36.5 86-87 28.484885840866795 34.0 29.0 35.0 2.0 36.0 88-89 28.062213657532048 34.0 29.0 35.0 2.0 36.0 90-91 27.56270250611651 34.0 27.0 35.0 2.0 35.0 92-93 27.15913910694212 33.5 26.5 35.0 2.0 35.0 94-95 26.73182050046759 33.0 25.5 35.0 2.0 35.0 96-97 26.22476525821596 33.0 25.0 35.0 2.0 35.0 98-99 25.49422591889364 33.0 22.0 34.5 2.0 35.0 100-101 23.24463329271403 30.5 2.0 33.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.21346308916411516 1101 2 0.17589869470693031 1101 3 0.15700331919143906 1101 4 0.0942032174991212 1101 5 -0.013861124198086827 1101 6 0.05487630124495979 1101 7 0.1225094510840421 1101 8 0.06510845058360815 1101 9 0.30297467432696834 1101 10-11 0.11077686123075381 1101 12-13 0.3015687167236223 1101 14-15 0.38005280599678315 1101 16-17 0.18920590099208567 1101 18-19 0.34061205715099874 1101 20-21 0.3746175599520285 1101 22-23 0.38734748384606377 1101 24-25 0.2827506074850561 1101 26-27 0.22286989530297774 1101 28-29 0.0779340995172717 1101 30-31 0.23446672406514324 1101 32-33 0.40895196355253915 1101 34-35 0.2617207335088949 1101 36-37 0.24290576387879526 1101 38-39 0.3011438257500103 1101 40-41 0.2737706323056841 1101 42-43 0.1710833263504199 1101 44-45 0.2985854480352046 1101 46-47 0.3147378570439443 1101 48-49 0.3312340248500476 1101 50-51 0.6403837718251921 1101 52-53 0.6918327815393326 1101 54-55 0.5656707692163181 1101 56-57 0.7583035171193657 1101 58-59 0.82549689137754 1101 60-61 0.8450911847444118 1101 62-63 0.7197786795084014 1101 64-65 0.5770068044726813 1101 66-67 0.840793703965609 1101 68-69 0.650187559590627 1101 70-71 0.7168350848969283 1101 72-73 0.9676451388127241 1101 74-75 0.9110438358447901 1101 76-77 0.6941541021338544 1101 78-79 0.7320629705392356 1101 80-81 0.970886154690259 1101 82-83 0.7591530971974727 1101 84-85 1.0123727402323937 1101 86-87 1.1032058310109356 1101 88-89 0.9606203956362478 1101 90-91 0.9679343273835741 1101 92-93 1.207828512073391 1101 94-95 1.2154561922930647 1101 96-97 1.599344921191058 1101 98-99 1.781043359490063 1101 100-101 2.2405277122366734 1102 1 0.22109541038528135 1102 2 0.23688813113190577 1102 3 -0.004049719910383942 1102 4 0.03257775430571286 1102 5 -0.21510828973097063 1102 6 -0.10363263078313167 1102 7 0.0807529110696592 1102 8 -0.025995470659687214 1102 9 0.16015901147645906 1102 10-11 0.12542621690798228 1102 12-13 -0.005127539014445404 1102 14-15 -0.10498673827621019 1102 16-17 -0.0126634646403474 1102 18-19 -0.14401960039005957 1102 20-21 0.07550523209405213 1102 22-23 0.11044769954209954 1102 24-25 0.08259574879360798 1102 26-27 0.0015947998989602752 1102 28-29 -0.05156648120281915 1102 30-31 0.16811945344958446 1102 32-33 0.18616976124071982 1102 34-35 -0.1440712580844874 1102 36-37 -0.537091194254053 1102 38-39 -0.38970568478590906 1102 40-41 -0.30756474737464146 1102 42-43 -0.08878200189469965 1102 44-45 -0.18063306863975725 1102 46-47 -0.38346744133556143 1102 48-49 -0.4961745808686615 1102 50-51 -0.21471965165802231 1102 52-53 -0.41849712278011 1102 54-55 -0.17742972301309834 1102 56-57 0.016198545049235236 1102 58-59 -0.00621180836252222 1102 60-61 -0.10184206009824237 1102 62-63 -0.2949052428410255 1102 64-65 -0.3160162584110111 1102 66-67 -0.18195807967467914 1102 68-69 -0.18419660057750065 1102 70-71 -0.08307918975602391 1102 72-73 -0.22542355207826503 1102 74-75 -0.1470350867811696 1102 76-77 -0.10208227859730101 1102 78-79 -0.0460950406327143 1102 80-81 -0.09572382263400314 1102 82-83 -0.2640687931846948 1102 84-85 -1.3348176860361605E-4 1102 86-87 0.1831585438033727 1102 88-89 0.010265050246054841 1102 90-91 -0.0894601751328814 1102 92-93 0.21557144664559758 1102 94-95 0.04985417913007595 1102 96-97 -0.21949806315715392 1102 98-99 -0.19561031010785612 1102 100-101 0.7013360192993368 1103 1 0.15021105664379064 1103 2 0.25570899280991455 1103 3 0.16833123247057102 1103 4 0.18355281099506016 1103 5 -0.14830556864253452 1103 6 0.06512020368398908 1103 7 0.13063953238485482 1103 8 0.07668026630176428 1103 9 0.3505898504787268 1103 10-11 0.1883513869760023 1103 12-13 0.11215137255017993 1103 14-15 0.3125189306580296 1103 16-17 0.11942270316011161 1103 18-19 0.1298125991564234 1103 20-21 0.15602677404417165 1103 22-23 0.18223908276205236 1103 24-25 0.2740243202221819 1103 26-27 0.22637938039782313 1103 28-29 0.33779859816225155 1103 30-31 0.2762146915448227 1103 32-33 -0.08718624782957818 1103 34-35 -0.10701910388947766 1103 36-37 -0.007839493573776224 1103 38-39 0.12482946260637817 1103 40-41 0.07093865398589827 1103 42-43 0.040310968626840804 1103 44-45 0.1504553667343913 1103 46-47 0.5205915155805343 1103 48-49 0.39967575926739585 1103 50-51 0.4018336363238362 1103 52-53 0.02377045091603236 1103 54-55 -0.13693085679994255 1103 56-57 0.11513278541204386 1103 58-59 -0.011427227863649136 1103 60-61 0.11706950452761333 1103 62-63 0.2184507662292674 1103 64-65 0.35765721097674685 1103 66-67 0.4427584736133028 1103 68-69 -0.09458208810584878 1103 70-71 0.2358323748698261 1103 72-73 0.3428077404387402 1103 74-75 0.17037987920522468 1103 76-77 0.15317849237775505 1103 78-79 0.2518868187777201 1103 80-81 0.3692194880235924 1103 82-83 0.2318495741622435 1103 84-85 0.243063797142959 1103 86-87 0.04256897464237497 1103 88-89 0.18768001623244857 1103 90-91 0.15736522169251543 1103 92-93 0.13080954188369276 1103 94-95 0.3212827505586482 1103 96-97 0.3125427531693781 1103 98-99 0.6056368554250255 1103 100-101 0.9691591485510394 1104 1 0.10159682443404705 1104 2 0.037107244994679434 1104 3 0.06468578927456292 1104 4 -0.0324022451847199 1104 5 -0.36910456989097185 1104 6 -0.14064758657818288 1104 7 -0.17572750881365096 1104 8 -0.014306000864273472 1104 9 -0.013180436662345585 1104 10-11 -0.09335897507143187 1104 12-13 -0.038809925826846836 1104 14-15 2.5925525228132074E-4 1104 16-17 -0.17914159147159836 1104 18-19 -0.07313371794719359 1104 20-21 0.035415038713331626 1104 22-23 0.15007928251236535 1104 24-25 -0.0623302365818148 1104 26-27 -0.15817742359718068 1104 28-29 -0.06258841806745608 1104 30-31 -0.2518377429121017 1104 32-33 -0.3184596560692725 1104 34-35 -0.26874195033142456 1104 36-37 -0.36302051729412455 1104 38-39 -0.4624489393911233 1104 40-41 -0.6560526069379407 1104 42-43 -0.4769549489761573 1104 44-45 -0.5146008130408859 1104 46-47 -0.7068616679400677 1104 48-49 -0.7813105078986524 1104 50-51 -0.8361189229770289 1104 52-53 -0.7729087001451376 1104 54-55 -0.5961817931295244 1104 56-57 -0.5500732070973129 1104 58-59 -0.7579940193742658 1104 60-61 -0.8784860510279415 1104 62-63 -0.6317240152938268 1104 64-65 -0.7997585193603314 1104 66-67 -0.8099256712056757 1104 68-69 -0.8315608646351862 1104 70-71 -0.8407469010352919 1104 72-73 -0.5846454430818575 1104 74-75 -0.462179421668683 1104 76-77 -0.6718776873975258 1104 78-79 -0.6762155532572365 1104 80-81 -0.6984958677816522 1104 82-83 -0.44443007627470976 1104 84-85 -0.6488837684001112 1104 86-87 -0.6206020615623693 1104 88-89 -0.5580628052407093 1104 90-91 -0.42784077081684657 1104 92-93 -0.4355075617367845 1104 94-95 -0.43814296729403424 1104 96-97 -0.24791916942737657 1104 98-99 0.09496269812165536 1104 100-101 0.6499206966159576 1105 1 0.1917828723619479 1105 2 0.4137035727557148 1105 3 0.32231497230797146 1105 4 0.16398641533110236 1105 5 0.07082722268538078 1105 6 0.1795375478574286 1105 7 0.22278045379406564 1105 8 0.2134824343234456 1105 9 0.2914570591508152 1105 10-11 0.38144081787032036 1105 12-13 0.44689391997565764 1105 14-15 0.5575595810645311 1105 16-17 0.5374443833769149 1105 18-19 0.5706256072865088 1105 20-21 0.4877611913883442 1105 22-23 0.46559951636638885 1105 24-25 0.3328861088400643 1105 26-27 0.4142655592596114 1105 28-29 0.15110483122457907 1105 30-31 0.2964585939838429 1105 32-33 0.31376226165552623 1105 34-35 0.10013536765523412 1105 36-37 0.1355209400305597 1105 38-39 0.2598159124708772 1105 40-41 0.18129095750893498 1105 42-43 0.2940508060252114 1105 44-45 0.5865258274389973 1105 46-47 0.6772039817051976 1105 48-49 0.43861884869829026 1105 50-51 0.5140287582750602 1105 52-53 0.8127677408889298 1105 54-55 1.0859959724683463 1105 56-57 0.9900108341925318 1105 58-59 0.8116080024886543 1105 60-61 0.690619640028963 1105 62-63 1.0666621483430916 1105 64-65 1.1616951513561489 1105 66-67 0.7083411294398516 1105 68-69 0.5865019227342572 1105 70-71 0.3774041905879848 1105 72-73 0.15904080276936483 1105 74-75 0.24132838869032014 1105 76-77 0.21515681216095217 1105 78-79 0.5616700166096962 1105 80-81 0.3926341221699339 1105 82-83 0.43022355790208167 1105 84-85 0.6055299218042087 1105 86-87 0.36641721312475894 1105 88-89 0.4663792032243208 1105 90-91 0.5959831078713798 1105 92-93 0.8135195689839634 1105 94-95 1.0047515852469964 1105 96-97 1.287691804659893 1105 98-99 1.110650405560527 1105 100-101 1.596557522534777 1106 1 -0.2454195739868439 1106 2 0.034913197014112995 1106 3 -0.012629728490388459 1106 4 -0.1153661079238617 1106 5 -0.1140863494233173 1106 6 -0.13708700352322012 1106 7 -0.11823434148902123 1106 8 -0.07351492889343092 1106 9 0.02141567630455654 1106 10-11 0.06260063622526246 1106 12-13 -0.027630909732103248 1106 14-15 0.012413825552918922 1106 16-17 0.06630708754449444 1106 18-19 0.06804833489216122 1106 20-21 -0.14973148421408666 1106 22-23 -0.3859441004211277 1106 24-25 -0.15190661070874967 1106 26-27 -0.32374073972229667 1106 28-29 -0.4737029033392517 1106 30-31 -0.5470510742209456 1106 32-33 -0.39304210368543124 1106 34-35 -0.5711182029885791 1106 36-37 -0.84233649057078 1106 38-39 -0.7780008677239536 1106 40-41 -0.7626499346026847 1106 42-43 -0.8895764187605408 1106 44-45 -1.0659635521845274 1106 46-47 -0.8036427186536983 1106 48-49 -0.8695734899818532 1106 50-51 -1.3116048021145943 1106 52-53 -1.443068888423312 1106 54-55 -1.4527867126558007 1106 56-57 -1.4595744217951605 1106 58-59 -1.3615773780138056 1106 60-61 -1.2332157807576678 1106 62-63 -1.1494396241611184 1106 64-65 -1.198221167401634 1106 66-67 -1.1852445293897027 1106 68-69 -2.134214220237979 1106 70-71 -1.9530039614665107 1106 72-73 -1.4000075748765752 1106 74-75 -1.1477207213953768 1106 76-77 -1.552632318433055 1106 78-79 -1.6149074755165742 1106 80-81 -1.6118841155800112 1106 82-83 -1.3089912666785963 1106 84-85 -1.4540082749291088 1106 86-87 -1.6490601544867545 1106 88-89 -1.4847899537375184 1106 90-91 -1.190382526055231 1106 92-93 -1.0024412088670616 1106 94-95 -0.7668553875794899 1106 96-97 -0.5891164059897811 1106 98-99 -0.6599036851155162 1106 100-101 0.15705704644893714 1107 1 0.3512198285736119 1107 2 0.2777119167865223 1107 3 0.18224936112554246 1107 4 0.1752633373108452 1107 5 -0.1827207733208951 1107 6 -0.03404646298267977 1107 7 -0.08427274831689857 1107 8 0.12314225460585959 1107 9 0.29061909024481025 1107 10-11 0.25103413551401843 1107 12-13 0.11612798073731057 1107 14-15 0.3125116207165064 1107 16-17 0.35973703064549056 1107 18-19 0.29960061085233036 1107 20-21 0.3654712184886151 1107 22-23 0.31386919972111826 1107 24-25 0.29017198104089204 1107 26-27 -0.018130853520304413 1107 28-29 0.11533514786985677 1107 30-31 0.3245260950535993 1107 32-33 0.3931865591879671 1107 34-35 0.21164317681228084 1107 36-37 0.43189003858996244 1107 38-39 0.3251730298578437 1107 40-41 0.5432266656818072 1107 42-43 0.8700697405566657 1107 44-45 0.8217053667343919 1107 46-47 0.7754380068086064 1107 48-49 0.8975851159925412 1107 50-51 1.0032956246279312 1107 52-53 0.8160438427289023 1107 54-55 0.7709419502176047 1107 56-57 1.074387171376955 1107 58-59 1.019201427107106 1107 60-61 0.9701981594983735 1107 62-63 0.730716848100613 1107 64-65 0.9877230004504298 1107 66-67 0.8694763098706133 1107 68-69 0.4642921809000029 1107 70-71 0.4405326672674903 1107 72-73 0.5315358106141801 1107 74-75 0.9757234464566871 1107 76-77 0.6989679660619679 1107 78-79 0.9730637193625142 1107 80-81 0.7817414178481528 1107 82-83 0.7924489893669211 1107 84-85 0.712910288371031 1107 86-87 0.7401201442330176 1107 88-89 0.9005674431330348 1107 90-91 0.9877380287100621 1107 92-93 1.0544133430532838 1107 94-95 0.6055663762896408 1107 96-97 0.7535222853331192 1107 98-99 1.1026763291092365 1107 100-101 1.8432234760364175 1108 1 0.2749484628602943 1108 2 0.11898159016610776 1108 3 0.11946377980890333 1108 4 0.1990512032780174 1108 5 0.05714637848144122 1108 6 0.19645996788548814 1108 7 0.21209719583608688 1108 8 0.20532621128212014 1108 9 0.2483319012111309 1108 10-11 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0.09890884081272588 2316 96-97 0.13358560489482585 2316 98-99 -0.04299795686166519 2316 100-101 -0.28824320260558167 >>END_MODULE >>Per sequence quality scores pass #Quality Count 4 1.0 5 9.0 6 11.0 7 36.0 8 94.0 9 228.0 10 310.0 11 683.0 12 1251.0 13 2055.0 14 2558.0 15 3723.0 16 5737.0 17 9261.0 18 8557.0 19 3886.0 20 2814.0 21 2443.0 22 2441.0 23 2437.0 24 2631.0 25 3066.0 26 3492.0 27 4060.0 28 5094.0 29 6245.0 30 7611.0 31 10053.0 32 12766.0 33 17563.0 34 25456.0 35 38819.0 36 60318.0 37 92953.0 38 77379.0 39 7402.0 40 1.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 46.813517593994916 19.350441001698794 15.096127265553195 18.739914138753097 2 15.399911204315092 22.2733112288756 40.468397237887416 21.85838032892189 3 20.803931570644522 27.501393336545092 30.86098752137237 20.833687571438016 4 12.004893209019373 17.670341296605926 38.0503679353114 32.2743975590633 5 11.805316088560538 40.08748352598289 33.24137800724621 14.865822378210366 6 28.555842094822452 37.75540567347748 17.560055166680836 16.128697065019225 7 26.28470352632225 36.777708504548414 19.89235884792322 17.04522912120611 8 26.676963187576163 33.82572429884471 19.15837749501705 20.338935018562076 9 27.808163535201817 17.225418237122263 20.720567536675453 34.24585069100046 10-11 24.68685351545895 28.147759798225973 25.571976459697147 21.593410226617923 12-13 27.54402206771463 26.262564238210402 26.96044626662636 19.23296742744861 14-15 23.749893728568594 26.232748604301868 28.584063063828985 21.433294603300553 16-17 19.580393157064453 29.99664654594232 29.255108113469554 21.167852183523678 18-19 19.352027658911215 27.284835775214667 34.27678748547624 19.08634908039788 20-21 21.002304909267814 25.54186622079897 34.387900170978924 19.06792869895429 22-23 20.326986930913797 25.900831754998322 33.98257612612826 19.789605187959626 24-25 20.276402469768477 25.861956992777024 34.71007142680022 19.151569110654272 26-27 20.41035862689507 25.654026773916627 35.000690779801836 18.93492381938647 28-29 19.400616387403026 26.449514093072136 35.26432628384522 18.88554323567962 30-31 19.577320598004682 26.40246645640543 34.844246489774854 19.17596645581503 32-33 19.408587676292495 26.642602091421768 34.509639999622145 19.43917023266359 34-35 20.17397814114735 26.050434059757606 34.51034847583151 19.265239323263526 36-37 20.465988418775563 25.865521769112327 34.610007462616075 19.058482349496035 38-39 20.29406485863538 26.176306666288813 34.72702411653017 18.80260435854564 40-41 19.911369626207954 26.62536250366046 34.43607655321601 19.02719131691558 42-43 19.66273027869627 27.154463863958433 33.64277279168635 19.540033065658953 44-45 20.122849774704566 27.00734925987852 32.94556068807209 19.924240277344822 46-47 20.54026034139107 27.639428118003796 31.924764549739752 19.89554699086538 48-49 21.030053560801427 27.835794107367207 31.31996202567518 19.814190306156185 50-51 20.84951020678059 28.503178696592702 31.155359386365138 19.491951710261567 52-53 20.776419077847365 29.111759760440574 30.442278081635354 19.669543080076703 54-55 19.95742057981693 28.767204163950844 30.573936577209736 20.70143867902249 56-57 20.798617999074256 29.015761234071093 29.948706322441694 20.236914444412957 58-59 21.165372516790885 28.418870027677805 30.038446642295085 20.377310813236225 60-61 20.601071216028565 28.260525594883855 31.245926261796132 19.892476927291447 62-63 21.048592021613246 27.990241921009623 31.01992235100745 19.941243706369676 64-65 20.75587326777567 28.62255693787136 30.815290805868074 19.806278988484898 66-67 20.425605274841537 28.53718555464241 30.672296690943785 20.36491247957227 68-69 20.46055676783707 28.836634832468995 30.313217332161983 20.389591067531953 70-71 20.718324028679117 29.19902041356118 29.886714654121917 20.195940903637787 72-73 20.882454350516245 28.54462455484078 29.959923862423366 20.61299723221961 74-75 20.475434768233818 29.53873475595356 29.6198552819263 20.365975193886324 76-77 20.792359792558166 28.579103730363403 30.03821048355863 20.590325993519805 78-79 20.689040345358535 28.63731685889988 30.071272706662512 20.602370089079074 80-81 20.48370032400979 29.295137019298895 29.789181095965468 20.431981560725855 82-83 20.359362749265546 29.389836672617868 29.515355041044387 20.7354455370722 84-85 20.214007046976697 29.08731733121735 29.586320741349503 21.112354880456447 86-87 20.467405371194303 29.21177298532982 29.352051274784856 20.96877036869102 88-89 20.545455833593106 28.862021896638048 29.12321345915871 21.46930881061014 90-91 20.416848718160672 28.617077807824476 29.41192447126342 21.554149002751437 92-93 20.60781609412565 28.984210053987162 29.44543895860984 20.962534893277347 94-95 20.115883091979107 28.954005724487768 29.423725451299344 21.50638573223378 96-97 19.867798339331767 28.873475595356172 29.370943973701362 21.887782091610696 98-99 20.287028006130694 29.18432941387625 28.57055140833595 21.958091171657106 100-101 20.077067786822425 29.455577376601198 28.865582490047675 21.6017723465287 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 689.0 1 457.5 2 322.0 3 420.0 4 363.0 5 344.5 6 555.5 7 1075.5 8 1735.0 9 2093.0 10 1909.0 11 1612.0 12 1638.0 13 1702.5 14 1715.0 15 1780.0 16 1854.5 17 1933.0 18 1979.0 19 2031.0 20 2070.5 21 2283.0 22 2610.5 23 2907.5 24 3214.5 25 3599.0 26 4155.0 27 4757.0 28 5299.5 29 5942.5 30 6593.5 31 7081.5 32 7840.5 33 8740.5 34 9434.5 35 9993.0 36 10842.0 37 11649.0 38 11965.5 39 11900.0 40 11849.0 41 12270.5 42 12498.0 43 12909.5 44 13649.5 45 13808.5 46 14004.5 47 15895.5 48 22621.5 49 22062.0 50 15215.0 51 12710.5 52 10782.5 53 9976.5 54 9517.5 55 8634.5 56 7489.0 57 6747.5 58 6295.5 59 5711.0 60 4846.5 61 3844.5 62 3014.0 63 2248.0 64 1557.5 65 1184.5 66 876.5 67 629.5 68 451.5 69 295.5 70 199.0 71 152.5 72 146.5 73 151.0 74 135.5 75 107.5 76 79.0 77 44.5 78 31.0 79 25.0 80 17.0 81 10.5 82 3.5 83 0.0 84 0.0 85 0.5 86 0.5 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.5 94 0.5 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.04794022350062818 2 0.0 3 0.0 4 0.0 5 0.011807936822814823 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.004723174729125929 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 4.723174729125929E-4 24-25 8.265555775970376E-4 26-27 0.0022435079963348164 28-29 0.002125428628106668 30-31 5.903968411407411E-4 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.010390984404077045 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 0.0 84-85 0.0 86-87 0.0 88-89 0.0 90-91 0.006848603357232597 92-93 0.0023615873645629645 94-95 0.0 96-97 0.0 98-99 2.3615873645629646E-4 100-101 0.011926016191042972 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 423444.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 59.663288050597394 #Duplication Level Percentage of deduplicated Percentage of total 1 81.41848638009166 48.57694605539049 2 11.124987830132028 13.275067069371152 3 3.335145853304513 5.969573032093879 4 1.3579703531170288 3.24083905368771 5 0.7422003158944771 2.214105561922829 6 0.44088600097373 1.578282508414297 7 0.28451661774620135 1.1882637843841326 8 0.20919809291315244 0.9985156861690442 9 0.15771486250157718 0.8468808544162765 >10 0.8342489624626901 8.824760140745338 >50 0.05494206834191244 2.1996261871851743 >100 0.03248399660814461 4.20544216940839 >500 0.0040103699516227925 1.5665128517931683 >1k 0.002406221970973675 2.7140606809057446 >5k 8.020739903245583E-4 2.601124364112347 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5867 1.3855433067890914 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5004 1.1817383172273075 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3416 0.8067182437347087 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 2391 0.5646555388670048 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 1708 0.40335912186735434 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 1617 0.3818686768498314 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1209 0.28551591237566243 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1002 0.23663105392920905 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 939 0.22175305353246239 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 865 0.20427730703469643 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 840 0.19837333862328904 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 595 0.1405144481914964 No Hit CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA 583 0.13768054335402083 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 566 0.1336658448342638 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 559 0.13201273367906974 No Hit GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT 545 0.12870651136868158 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 530 0.12516413032183712 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 525 0.12398333663955563 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 499 0.11784320949169193 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 492 0.11619009833649786 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 477 0.11264771728965342 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 466 0.11004997118863416 No Hit GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA 462 0.10910533624280898 No Hit TGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC 461 0.10886917750635268 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 446 0.10532679645950822 No Hit CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG 434 0.10249289162203266 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 2.3615873645629646E-4 0.0 0.0 0.0 0.0 20-21 2.3615873645629646E-4 0.0 0.0 1.1807936822814823E-4 0.0 22-23 2.3615873645629646E-4 0.0 0.0 5.903968411407411E-4 0.0 24-25 4.723174729125929E-4 0.0 0.0 7.084762093688894E-4 0.0 26-27 4.723174729125929E-4 0.0 0.0 0.0014169524187377788 0.0 28-29 7.084762093688894E-4 0.0 0.0 0.003424301678616299 0.0 30-31 7.084762093688894E-4 0.0 0.0 0.009800587562936303 0.0 32-33 7.084762093688894E-4 0.0 0.0 0.01723958776130964 0.0 34-35 7.084762093688894E-4 0.0 0.0 0.02491474669613928 0.0 36-37 9.446349458251858E-4 0.0 0.0 0.03495149299553188 0.0 38-39 9.446349458251858E-4 0.0 0.0 0.04156393761630818 0.0 40-41 9.446349458251858E-4 0.0 0.0 0.046995588554803 0.0 42-43 0.001062714314053334 0.0 0.0 0.055615382435457814 0.0 44-45 0.0011807936822814823 0.0 0.0 0.06317246200205931 0.0 46-47 0.0014169524187377788 0.0 0.0 0.07616119250715561 0.0 48-49 0.0014169524187377788 0.0 0.0 0.08997647858984895 0.0 50-51 0.0014169524187377788 0.0 0.0 0.10107593920329488 0.0 52-53 0.0014169524187377788 0.0 0.0 0.11772513012346379 0.0 54-55 0.0014169524187377788 0.0 0.0 0.16129641699965047 0.0 56-57 0.0014169524187377788 0.0 0.0 0.25127289558949945 0.0 58-59 0.0014169524187377788 0.0 0.0 0.34644486638138694 0.0 60-61 0.0014169524187377788 0.0 0.0 0.4294546622457751 0.0 62-63 0.0014169524187377788 0.0 0.0 0.5338368237594582 0.0 64-65 0.0014169524187377788 0.0 0.0 0.6548681761933101 0.0 66-67 0.0014169524187377788 0.0 0.0 0.8330499428495858 0.0 68-69 0.0014169524187377788 0.0 0.0 1.0582272980606644 0.0 70-71 0.0014169524187377788 0.0 0.0 1.2733679069723505 0.0 72-73 0.0014169524187377788 0.0 0.0 1.5104712783744723 0.0 74-75 0.0014169524187377788 0.0 0.0 1.7527701419786323 0.0 76-77 0.0014169524187377788 0.0 0.0 2.0313193716288342 0.0 78-79 0.0014169524187377788 0.0 0.0 2.253308583897753 0.0 80-81 0.0016531111551940753 0.0 0.0 2.4435344461132997 0.0 82-83 0.0016531111551940753 0.0 0.0 2.6720180236347666 0.0 84-85 0.0018892698916503717 0.0 0.0 3.0273188426332642 0.0 86-87 0.0018892698916503717 0.0 0.0 3.4392977583812736 0.0 88-89 0.0018892698916503717 0.0 0.0 3.9780939156063138 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTAAGG 135 1.19362085E-8 31.665918 3 CTACGTC 75 3.0756157E-4 31.665918 3 TGCACCG 65 0.005840631 29.23008 5 ATGGGAG 740 0.0 28.884453 5 ACCGTGC 85 6.39691E-4 27.943817 8 ATGGACG 70 0.0083866585 27.142216 5 ACGTCTA 90 8.9394115E-4 26.388266 5 TAAGGTG 185 8.936695E-9 25.67507 5 TACCTGG 385 0.0 24.674742 2 AGGTAAG 175 1.4818579E-7 24.427996 2 ACTTAGC 125 2.2511953E-4 22.802155 8 GAGTACT 4640 0.0 22.77963 12-13 GTTCTAG 190 3.2589196E-7 22.51276 1 GTAAGGT 170 3.1418476E-6 22.352411 4 CGACTAC 85 3.1480085E-6 22.352411 88-89 AGTACTT 4745 0.0 22.325607 12-13 TATAGAC 130 2.9377305E-4 21.92256 2 TACGTCT 130 2.9377305E-4 21.92256 4 GTACATG 3865 0.0 21.765247 1 ATAGGTG 175 4.0400882E-6 21.713774 5 >>END_MODULE