FastQCFastQC Report
Thu 26 May 2016
SRR937695_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937695_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences124759
Sequences flagged as poor quality0
Sequence length101
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17601.4107198679053214No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13311.0668569001033994No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9110.7302078407169021No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT3150.2524867945398729No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2870.2300435239141064No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT2860.22924197853461475No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2740.21962343398071482No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2390.19156934569850673No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT2380.19076780031901505No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC2180.17473689272918186No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1600.12824726071866557No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1490.11943026154425734No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1390.11141480774934072No Hit
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT1330.10660553547239078No Hit
GTCCCAGGGTGTGCTTGTCAAAGAGATATTCTGCCATGCCAGCTTCAGGG1310.10500244471340744No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA6950.044.4441641
GTATCAA12000.038.8088721
GTGCAGA651.3179558E-436.554671
GTCCTAT550.002565260734.5607761
CTGGACA550.002565260734.5607764
GTGTGGG600.003929025531.6807121
TAAGGTG753.0548804E-431.6807125
GACGTAT300.003952009631.65530862-63
ATCAACG15000.031.0470963
TCAACGC14850.031.0406994
TATCAAC15350.030.6487662
CAACGCA15000.030.4134835
GTACATG8200.030.135311
AACGCAG15150.030.100286
CATGGGG1901.0913936E-1130.0133064
TTCCTAC804.4593378E-429.7006681
TACATGG8450.029.2437342
AGAGCCC650.005811749529.2437345
ACATGGG8700.028.4033973
CCTACCC700.00834531827.1548963
Produced by FastQC (version 0.11.5)