##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937695_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 124759 Sequences flagged as poor quality 0 Sequence length 101 %GC 41 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.928293750350676 33.0 31.0 34.0 30.0 34.0 2 32.05052942072315 33.0 31.0 34.0 30.0 34.0 3 32.02167378706145 34.0 31.0 34.0 30.0 34.0 4 35.615554789634416 37.0 35.0 37.0 35.0 37.0 5 35.62451606697713 37.0 35.0 37.0 35.0 37.0 6 35.73403121217708 37.0 35.0 37.0 35.0 37.0 7 35.69966896175827 37.0 35.0 37.0 35.0 37.0 8 35.697144093812874 37.0 35.0 37.0 35.0 37.0 9 37.34280492790099 39.0 37.0 39.0 35.0 39.0 10-11 37.22859673450412 39.0 37.0 39.0 34.0 39.0 12-13 37.11124247549275 39.0 37.0 39.0 34.0 39.0 14-15 38.23229987415738 40.0 38.0 41.0 33.5 41.0 16-17 38.18892023821929 40.0 38.0 41.0 33.5 41.0 18-19 38.25633020463454 40.0 38.0 41.0 34.0 41.0 20-21 38.20582082254587 40.0 38.0 41.0 34.0 41.0 22-23 38.11625614184147 40.0 38.0 41.0 34.0 41.0 24-25 38.09678259684672 40.0 38.0 41.0 34.0 41.0 26-27 37.96004296283234 40.0 38.0 41.0 33.5 41.0 28-29 37.811003614969664 40.0 38.0 41.0 33.0 41.0 30-31 37.47819796567782 39.5 37.0 41.0 33.0 41.0 32-33 37.196731297942435 39.0 36.0 41.0 33.0 41.0 34-35 37.107924077621654 40.0 36.0 41.0 32.5 41.0 36-37 36.91767327407241 40.0 35.0 41.0 32.0 41.0 38-39 36.72210421693024 39.0 35.0 41.0 31.0 41.0 40-41 36.43566396011511 39.0 35.0 41.0 30.5 41.0 42-43 36.03719170560841 39.0 35.0 41.0 30.0 41.0 44-45 35.60891398616533 39.0 35.0 41.0 28.0 41.0 46-47 34.98931139236448 39.0 35.0 41.0 24.0 41.0 48-49 34.442573281286315 39.0 35.0 40.0 20.5 41.0 50-51 33.46101684046842 38.0 33.5 39.5 15.0 40.5 52-53 33.4903814554461 38.0 33.5 39.5 11.5 40.5 54-55 33.70847393775199 38.0 34.0 40.0 8.0 41.0 56-57 33.47596966952284 38.0 34.0 40.0 4.5 41.0 58-59 33.20574868346172 38.0 33.5 40.0 2.0 41.0 60-61 32.8356992281118 38.0 33.0 40.0 2.0 41.0 62-63 32.45165479043596 37.0 33.0 40.0 2.0 41.0 64-65 32.067710545932556 37.0 32.0 40.0 2.0 41.0 66-67 31.583452897185772 36.0 31.5 39.0 2.0 41.0 68-69 31.093796840308116 35.5 31.0 39.0 2.0 40.5 70-71 30.63461553875873 35.0 30.5 39.0 2.0 40.0 72-73 30.166829647560498 35.0 30.0 38.0 2.0 40.0 74-75 29.66529468815877 35.0 29.0 37.0 2.0 39.5 76-77 29.18454780817416 34.5 29.0 37.0 2.0 39.0 78-79 28.603455462130988 34.0 28.0 36.0 2.0 39.0 80-81 28.05682155195216 34.0 26.5 36.0 2.0 38.0 82-83 27.56103768064829 34.0 26.0 35.0 2.0 37.0 84-85 27.06425588534695 34.0 25.5 35.0 2.0 37.0 86-87 26.545668047996536 33.0 24.5 35.0 2.0 36.0 88-89 26.097175354082673 33.0 23.5 35.0 2.0 36.0 90-91 25.545118989411584 32.5 20.0 35.0 2.0 35.5 92-93 24.964042674276005 32.0 16.5 35.0 2.0 35.0 94-95 24.22352295225194 32.0 4.5 34.0 2.0 35.0 96-97 23.476226164044277 31.0 2.0 34.0 2.0 35.0 98-99 22.475693136366914 31.0 2.0 34.0 2.0 35.0 100-101 20.01384669643072 27.5 2.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.22764460884455318 1101 2 0.12608005486951868 1101 3 0.19389570948888846 1101 4 0.12918508761521963 1101 5 0.3203116253347673 1101 6 -0.068815804879506 1101 7 0.13045207719424212 1101 8 0.1712191269649992 1101 9 0.5366581719007399 1101 10-11 0.2635468039774622 1101 12-13 0.37773684853179645 1101 14-15 0.638901717368114 1101 16-17 0.5849482058233448 1101 18-19 0.6020127260007158 1101 20-21 0.30414600589874397 1101 22-23 0.14766048909576313 1101 24-25 0.2177566112901559 1101 26-27 0.39935085425295824 1101 28-29 0.237291990043488 1101 30-31 0.6254667601167156 1101 32-33 0.6954095640258728 1101 34-35 0.7720971430790158 1101 36-37 1.0607650584165995 1101 38-39 1.0496469640110462 1101 40-41 1.2910821002886124 1101 42-43 1.1514785931993288 1101 44-45 1.2123985241345991 1101 46-47 1.0850667196158454 1101 48-49 1.380561356941314 1101 50-51 1.6404357175391056 1101 52-53 1.7777588238608644 1101 54-55 1.9207891060548405 1101 56-57 1.9305758070375774 1101 58-59 1.8776528833497892 1101 60-61 1.75397542315973 1101 62-63 2.1644674013016036 1101 64-65 1.9477806471942927 1101 66-67 1.613325475078483 1101 68-69 1.7528451200030233 1101 70-71 1.6224084581000966 1101 72-73 2.125485878109469 1101 74-75 1.7774750323244923 1101 76-77 1.8097593130846263 1101 78-79 1.90769207101388 1101 80-81 2.3499863806456602 1101 82-83 2.2044375538748504 1101 84-85 1.7525967750744904 1101 86-87 2.1520482733936035 1101 88-89 1.645357572353241 1101 90-91 2.027509939935115 1101 92-93 2.238862774527849 1101 94-95 2.3715037346323733 1101 96-97 2.498649620577229 1101 98-99 1.751467580501437 1101 100-101 1.5123158700099992 1102 1 -0.013818805789593824 1102 2 0.03297294988542632 1102 3 0.1702477773573925 1102 4 0.07139081402031167 1102 5 -0.0013214605613072195 1102 6 0.14157655991370177 1102 7 0.12621029564598985 1102 8 -0.10417854005515181 1102 9 0.3382488399813326 1102 10-11 -0.05941183865244426 1102 12-13 0.4513847806632896 1102 14-15 0.5756461500298258 1102 16-17 0.35043070847446245 1102 18-19 0.33303075357229517 1102 20-21 0.4427992402571732 1102 22-23 0.3717856216514335 1102 24-25 0.29241196653936186 1102 26-27 0.3436244491628173 1102 28-29 0.4391477694708428 1102 30-31 0.2181496869459849 1102 32-33 1.0442960963694503 1102 34-35 1.163613579982517 1102 36-37 1.2898742842914714 1102 38-39 1.1174624465136986 1102 40-41 1.279523245569628 1102 42-43 1.2617649134538382 1102 44-45 1.8347208995322646 1102 46-47 2.0292342699869934 1102 48-49 2.5994372848310263 1102 50-51 2.6835958447925563 1102 52-53 2.334174518452599 1102 54-55 2.4577986500633244 1102 56-57 2.101042403007888 1102 58-59 1.98396253340281 1102 60-61 2.014367787952949 1102 62-63 2.1885925338572783 1102 64-65 2.320792312093559 1102 66-67 2.4641738313881376 1102 68-69 2.2376807509680283 1102 70-71 2.3842324241658446 1102 72-73 2.2454752736555967 1102 74-75 2.6684082242651073 1102 76-77 2.2926331200835683 1102 78-79 2.5481480625303163 1102 80-81 2.5526905163826683 1102 82-83 2.393514966388107 1102 84-85 2.2917802321264524 1102 86-87 2.5736813592896794 1102 88-89 2.672770085608807 1102 90-91 2.2142543725968338 1102 92-93 1.86277581800611 1102 94-95 2.3999236710056486 1102 96-97 2.9119051879155116 1102 98-99 3.072835555050748 1102 100-101 3.1118386695858185 1103 1 0.7048803812022761 1103 2 0.7093957141130716 1103 3 0.929597370853326 1103 4 0.760821708329253 1103 5 0.23445089716226875 1103 6 0.5448285924340297 1103 7 0.6091371249142838 1103 8 0.4958214599448496 1103 9 0.6667041245341778 1103 10-11 0.6535962914288547 1103 12-13 0.45788884570393407 1103 14-15 0.8813372069404011 1103 16-17 1.15530875725495 1103 18-19 0.8598600218649821 1103 20-21 1.0541813540783096 1103 22-23 0.7791026948221642 1103 24-25 0.8924119665393562 1103 26-27 1.0631366442847678 1103 28-29 1.1797981759749092 1103 30-31 1.2880683861329771 1103 32-33 1.5711253646621302 1103 34-35 1.5993859377060957 1103 36-37 1.8191425769744 1103 38-39 1.8280315522047559 1103 40-41 1.6762712130493043 1103 42-43 2.0308706045107456 1103 44-45 1.8989485418086929 1103 46-47 1.9641936195804917 1103 48-49 2.4319576100342815 1103 50-51 2.2209942187762977 1103 52-53 2.092711103818445 1103 54-55 2.726904341120232 1103 56-57 2.70592045178838 1103 58-59 2.9140438342158177 1103 60-61 3.177782422099284 1103 62-63 2.743877086702806 1103 64-65 3.1541256454268876 1103 66-67 3.060108790737729 1103 68-69 2.833615710317627 1103 70-71 2.4321999038406403 1103 72-73 2.1584834037368985 1103 74-75 2.3407659478423426 1103 76-77 2.1080802745551104 1103 78-79 2.9749773308229983 1103 80-81 2.5518775082525877 1103 82-83 2.3910759419978618 1103 84-85 2.558446898793118 1103 86-87 2.15498217229781 1103 88-89 2.227241630324251 1103 90-91 2.255717787230978 1103 92-93 1.7562717529654606 1103 94-95 2.33000497181866 1103 96-97 2.614344212305756 1103 98-99 2.1744615713109106 1103 100-101 2.123220783406957 1104 1 0.8560396705729438 1104 2 0.6485706614180984 1104 3 0.6357099875350478 1104 4 0.44013516813051723 1104 5 0.4870856457319732 1104 6 0.2918927297420666 1104 7 0.2541235747787809 1104 8 0.32478863850552386 1104 9 0.6742319775904875 1104 10-11 0.23007927848094312 1104 12-13 0.25012010134982887 1104 14-15 -0.20116806255673936 1104 16-17 0.3739176100101389 1104 18-19 0.452603171518696 1104 20-21 0.08941170638183138 1104 22-23 0.20909366139849794 1104 24-25 0.4964468957775381 1104 26-27 0.5807518204365323 1104 28-29 0.4455313888625909 1104 30-31 0.9711457724623926 1104 32-33 1.0221943197961352 1104 34-35 0.815736434544398 1104 36-37 0.32922990006984776 1104 38-39 0.19207852600782616 1104 40-41 0.2974696472558733 1104 42-43 0.3434270634086687 1104 44-45 -0.030891867706515086 1104 46-47 0.25326317694273115 1104 48-49 0.7186182544185016 1104 50-51 1.2702263777645513 1104 52-53 1.192680992406224 1104 54-55 1.4390392402470127 1104 56-57 1.583367004031679 1104 58-59 1.4985063455076002 1104 60-61 0.893079019509706 1104 62-63 1.277752425456196 1104 64-65 1.582912155514208 1104 66-67 1.3109368545739244 1104 68-69 1.4028719584356573 1104 70-71 0.2033531709288674 1104 72-73 0.9102148099398519 1104 74-75 1.2108352040904613 1104 76-77 1.163033601866161 1104 78-79 -0.05046681250732021 1104 80-81 -0.3719406188175718 1104 82-83 0.345607709537763 1104 84-85 0.889070205026183 1104 86-87 0.2395952406507149 1104 88-89 0.46180953155882065 1104 90-91 1.196699419269521 1104 92-93 0.3664795217098131 1104 94-95 0.5066084045196533 1104 96-97 1.4788428572922072 1104 98-99 0.5856329052464702 1104 100-101 1.0041301480561593 1105 1 0.418553699753641 1105 2 0.8059219521027217 1105 3 0.8678087529671465 1105 4 0.8758254038207554 1105 5 0.9649756569553176 1105 6 0.7841486219979643 1105 7 0.09250741316262179 1105 8 0.6040254510756711 1105 9 0.869069239094415 1105 10-11 0.6645349462699528 1105 12-13 0.6810965323262579 1105 14-15 0.5521428240874826 1105 16-17 0.8934462295387604 1105 18-19 0.765846718095581 1105 20-21 1.203553120523246 1105 22-23 1.2952889475937823 1105 24-25 0.7655826982466749 1105 26-27 1.036307375992088 1105 28-29 1.2675291441936807 1105 30-31 0.7163758510258091 1105 32-33 0.39817636244484333 1105 34-35 0.896207815015778 1105 36-37 1.3202512244245028 1105 38-39 1.1008327347620295 1105 40-41 0.8076828908024467 1105 42-43 1.228948948930551 1105 44-45 1.0402641367828167 1105 46-47 1.301074624754186 1105 48-49 -0.26331216084525266 1105 50-51 0.6937954013335741 1105 52-53 0.9042410372995988 1105 54-55 1.0500381179125498 1105 56-57 0.705476992808336 1105 58-59 0.5604592074604611 1105 60-61 0.6387580318553816 1105 62-63 0.975732223435994 1105 64-65 0.33453954047493184 1105 66-67 0.6945507715211718 1105 68-69 0.7117384006354364 1105 70-71 0.5081792090882402 1105 72-73 -0.09842716536879337 1105 74-75 -0.06817714158855281 1105 76-77 -0.07344226794304021 1105 78-79 0.19958930421546128 1105 80-81 0.22963064942036127 1105 82-83 0.22854822581161116 1105 84-85 1.3567469727029504 1105 86-87 0.4164751508639597 1105 88-89 0.46966587274849303 1105 90-91 0.845296501199936 1105 92-93 1.2900485452788395 1105 94-95 0.931187529098775 1105 96-97 1.4990448774942244 1105 98-99 1.6635229164698124 1105 100-101 1.0180470953064358 1106 1 0.8968753780753183 1106 2 1.123404469585239 1106 3 1.298577983736383 1106 4 0.8902776182729681 1106 5 0.07872857070823613 1106 6 0.03263347048280707 1106 7 0.9036962243514353 1106 8 0.8879415349917537 1106 9 0.8774608474860202 1106 10-11 1.0482179299529406 1106 12-13 0.6204904717202027 1106 14-15 0.4488794208240776 1106 16-17 0.6787609148534415 1106 18-19 1.2835623358111903 1106 20-21 1.1364202533903764 1106 22-23 0.8472702995751362 1106 24-25 1.4399416726056558 1106 26-27 1.3157945554792718 1106 28-29 1.5528438295083618 1106 30-31 2.0100821447321024 1106 32-33 1.4063348706033452 1106 34-35 0.6262777450857087 1106 36-37 1.066171970345259 1106 38-39 -0.12131178738248849 1106 40-41 0.2309929141124698 1106 42-43 0.09841281839442217 1106 44-45 0.7288422253609141 1106 46-47 0.9726363963159557 1106 48-49 1.4688556713225793 1106 50-51 1.0793431868813599 1106 52-53 1.39258602564459 1106 54-55 0.8388493067237377 1106 56-57 1.966083053414387 1106 58-59 1.8161701631714138 1106 60-61 1.5592708523682006 1106 62-63 1.0640772117809796 1106 64-65 2.230110636046028 1106 66-67 3.1276510046214128 1106 68-69 2.7495006383976737 1106 70-71 2.9944262953353302 1106 72-73 2.0887523218106985 1106 74-75 2.224130550719142 1106 76-77 2.8619889674881946 1106 78-79 2.382106786732944 1106 80-81 1.7272996470893602 1106 82-83 1.6509258481892317 1106 84-85 2.2523180682740467 1106 86-87 1.7372210716098806 1106 88-89 0.7016006046832288 1106 90-91 1.856718412621845 1106 92-93 1.4704681256984173 1106 94-95 2.2558961538074023 1106 96-97 1.3976462760956245 1106 98-99 0.07471172765862732 1106 100-101 -0.24325826599892153 1107 1 -0.134260153060211 1107 2 0.08860593478670609 1107 3 -0.09885791369951846 1107 4 -0.025473297477951462 1107 5 -0.39563040365074187 1107 6 -0.4911760533267113 1107 7 -0.49190817125295894 1107 8 -0.6267104796602609 1107 9 -0.06209959207441784 1107 10-11 -0.505811374076373 1107 12-13 -0.6533190520893299 1107 14-15 -0.4357359637913092 1107 16-17 -0.3377226016300767 1107 18-19 0.4309982332470952 1107 20-21 0.46242757939770485 1107 22-23 -0.03826083595600238 1107 24-25 -0.22489349222951205 1107 26-27 0.20773594742065882 1107 28-29 -0.11840159173705445 1107 30-31 -0.20786657321661295 1107 32-33 -0.47736476309628273 1107 34-35 -1.2583376395296781 1107 36-37 -0.9503115461382592 1107 38-39 -0.683582849653547 1107 40-41 -0.1169924338728805 1107 42-43 -0.5957263757447677 1107 44-45 0.10887885539754194 1107 46-47 -0.6171071934276355 1107 48-49 -1.9542212517543405 1107 50-51 -2.1477630402248664 1107 52-53 -0.921516538457972 1107 54-55 -1.515546297671868 1107 56-57 -1.4267740894427519 1107 58-59 -1.5226576756564256 1107 60-61 -2.2731467300493797 1107 62-63 -3.214311066607298 1107 64-65 -2.6132959573605667 1107 66-67 -2.971250094279693 1107 68-69 -3.06185467295764 1107 70-71 -2.412167111258082 1107 72-73 -3.017474784416411 1107 74-75 -1.991986665398077 1107 76-77 -2.3669487614495317 1107 78-79 -2.4521422975161435 1107 80-81 -2.666473246683534 1107 82-83 -3.33075913349575 1107 84-85 -2.8291837632277854 1107 86-87 -2.817723983335174 1107 88-89 -2.665615512532888 1107 90-91 -1.678080122176688 1107 92-93 -1.6586527534224587 1107 94-95 -1.5224921245808751 1107 96-97 -1.0171888887395397 1107 98-99 -2.273273620326723 1107 100-101 -2.4804377531784105 1108 1 0.3644867141077093 1108 2 -0.030441684260914315 1108 3 -0.30061229966442937 1108 4 0.27151918372506145 1108 5 0.30612398231417615 1108 6 -0.028770038289117394 1108 7 0.18102415957410756 1108 8 -0.04525684557504661 1108 9 -0.22375372741276323 1108 10-11 0.3049655682795205 1108 12-13 -0.20407093178857139 1108 14-15 -0.5234552620369186 1108 16-17 -0.2625346317052575 1108 18-19 -0.3497035211388706 1108 20-21 -0.1722842000509175 1108 22-23 0.2787817705602933 1108 24-25 -0.7712594070164798 1108 26-27 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2311 22-23 -0.07144219360544213 2311 24-25 -0.0982470889873639 2311 26-27 -0.2360330495398273 2311 28-29 -0.39069135870767013 2311 30-31 -0.21495877179817313 2311 32-33 -0.16758766076599585 2311 34-35 -0.9923801927211642 2311 36-37 -0.8277178277998658 2311 38-39 -0.5711208435745263 2311 40-41 -0.3145608229306305 2311 42-43 -0.49542242437699 2311 44-45 -0.20596410306243484 2311 46-47 -0.5426391083212465 2311 48-49 -0.5712425283500906 2311 50-51 -1.5398603046625539 2311 52-53 -1.4942115739189674 2311 54-55 -1.2609870271551458 2311 56-57 -1.7927821948125597 2311 58-59 -1.0302564598509534 2311 60-61 -1.3569866489956794 2311 62-63 -1.0993161324634286 2311 64-65 -1.5279362815753572 2311 66-67 -1.5938438126180898 2311 68-69 -1.570466628378103 2311 70-71 -1.1624204040645694 2311 72-73 -1.568639931326235 2311 74-75 -1.1277516096736555 2311 76-77 -1.0736356915407193 2311 78-79 -0.8895789743145208 2311 80-81 -1.8351662558020756 2311 82-83 -0.8728057393721436 2311 84-85 -0.2291331046664915 2311 86-87 -0.6419387756350723 2311 88-89 0.46483028280652405 2311 90-91 1.1638651665771107 2311 92-93 1.364903477580576 2311 94-95 1.5712262138183135 2311 96-97 1.749528436488422 2311 98-99 1.5362501891970872 2311 100-101 1.5851650837010212 2312 1 0.43533691653685835 2312 2 0.027250623431392285 2312 3 -0.7014220162636207 2312 4 0.23002120801655934 2312 5 0.033856775836440534 2312 6 0.23135141920075597 2312 7 0.23061930127450836 2312 8 0.09947999653021355 2312 9 0.6082300782552537 2312 10-11 -0.246653864918855 2312 12-13 -0.43079157956185554 2312 14-15 -0.5639410919964405 2312 16-17 -0.15777754668501132 2312 18-19 -0.16195048470162732 2312 20-21 0.7998817918519165 2312 22-23 0.6709882482930851 2312 24-25 0.792505775169758 2312 26-27 0.18438429906900922 2312 28-29 0.06245921412374855 2312 30-31 0.01969752934748925 2312 32-33 -1.0039215396530565 2312 34-35 -1.2775684087604517 2312 36-37 -2.12293059375731 2312 38-39 -1.9610553771260797 2312 40-41 -2.676058367938815 2312 42-43 -2.568253848272242 2312 44-45 -3.4794911079724216 2312 46-47 -5.607491808812249 2312 48-49 -5.636913559446647 2312 50-51 -5.904631172092998 2312 52-53 -6.312542179483614 2312 54-55 -6.363073770199335 2312 56-57 -6.184557972226635 2312 58-59 -7.007547785546535 2312 60-61 -6.145857352760004 2312 62-63 -5.9872048395010715 2312 64-65 -6.016684235748848 2312 66-67 -6.077477200506799 2312 68-69 -7.06780705391002 2312 70-71 -7.210701909792881 2312 72-73 -6.071504088445714 2312 74-75 -6.208561756973165 2312 76-77 -6.019421288922061 2312 78-79 -5.781354751728596 2312 80-81 -6.202187532397819 2312 82-83 -5.916381844118458 2312 84-85 -5.638707572751596 2312 86-87 -6.490343030954222 2312 88-89 -6.295194267111643 2312 90-91 -6.358025177121743 2312 92-93 -5.856454951224659 2312 94-95 -6.055001282090036 2312 96-97 -5.948507570058222 2312 98-99 -4.925288272341373 2312 100-101 -3.958001855742511 2313 1 -0.7802501279975544 2313 2 -0.701494315839863 2313 3 -0.6558754575591657 2313 4 -0.8600597636433633 2313 5 -0.3386128597910911 2313 6 -0.2919279330259599 2313 7 -0.3452916298995774 2313 8 -0.4268357929434714 2313 9 -0.8948063589917155 2313 10-11 -0.5042449580362671 2313 12-13 -0.48696566863067403 2313 14-15 -0.687928946247446 2313 16-17 -0.6375346317052646 2313 18-19 -0.7970719421915007 2313 20-21 -0.7643894632088148 2313 22-23 -0.20806033470287133 2313 24-25 -0.41599624912174704 2313 26-27 0.06393895493945934 2313 28-29 0.02602196716019023 2313 30-31 -0.5126911346201268 2313 32-33 0.04769789354531895 2313 34-35 -0.5609692184770481 2313 36-37 -0.5374042779678305 2313 38-39 -0.3856505188264805 2313 40-41 -1.7043984489104744 2313 42-43 -1.4923429170981493 2313 44-45 -2.1743291646525833 2313 46-47 -1.531580877638163 2313 48-49 -0.6121159885964431 2313 50-51 -0.8018983785707299 2313 52-53 -1.0509025033702564 2313 54-55 -0.9055838916568284 2313 56-57 -1.3935660693926266 2313 58-59 -2.0611915102428924 2313 60-61 -1.6296630207761957 2313 62-63 -1.537306054075973 2313 64-65 -1.6872307944532992 2313 66-67 -1.9894205203448578 2313 68-69 -1.6928070539100197 2313 70-71 -0.9829691162706133 2313 72-73 -0.411585060105633 2313 74-75 -0.4089666152727638 2313 76-77 -0.26638485167509884 2313 78-79 -0.444310217315639 2313 80-81 -0.01797700608203101 2313 82-83 0.09373961337141878 2313 84-85 0.06523979566945926 2313 86-87 0.3057096006247235 2313 88-89 -0.4788987205529409 2313 90-91 -0.062478618417287635 2313 92-93 0.7219863443218983 2313 94-95 0.3979339405820319 2313 96-97 0.6096908105085816 2313 98-99 1.2467765049865598 2313 100-101 0.6335973345408874 2314 1 -0.2154926460574096 2314 2 -0.28534364504522713 2314 3 -0.5596422274250088 2314 4 -0.2929803002790692 2314 5 0.016134302231613162 2314 6 -0.47717045108581857 2314 7 -0.04653001999245987 2314 8 -0.2779709838619411 2314 9 -0.29599314949739153 2314 10-11 -0.2572119343004573 2314 12-13 0.02735321681824132 2314 14-15 -0.30828498339914745 2314 16-17 -0.26279262964127525 2314 18-19 -0.5052762765568275 2314 20-21 -0.14751639819333207 2314 22-23 -0.13349893119409018 2314 24-25 -0.1422604390082256 2314 26-27 -0.45192791812555555 2314 28-29 -0.34459206792752894 2314 30-31 -0.2176704947852457 2314 32-33 -0.08310706001505253 2314 34-35 -0.5965729336473302 2314 36-37 -0.6596952996396581 2314 38-39 -0.7234988160400988 2314 40-41 -0.4937431341529859 2314 42-43 -0.7567908015150806 2314 44-45 -1.078095934518423 2314 46-47 -1.4847542522511645 2314 48-49 -1.3610839968523791 2314 50-51 -0.9138694828018927 2314 52-53 -1.568575361987385 2314 54-55 -1.323669546971587 2314 56-57 -0.9848973387424707 2314 58-59 -0.38820389414382106 2314 60-61 0.26816979656126705 2314 62-63 -0.3186528033019762 2314 64-65 -1.2295424559600079 2314 66-67 -0.8746114388175101 2314 68-69 -1.0163364656747227 2314 70-71 -0.5361166910900153 2314 72-73 -0.8592114790942809 2314 74-75 -1.2671967494316867 2314 76-77 -1.588237276855697 2314 78-79 -1.7532627456954124 2314 80-81 -1.4815992971037026 2314 82-83 -0.5142325228514899 2314 84-85 -0.7514526707908118 2314 86-87 -1.2771077368365766 2314 88-89 -1.0619740559502588 2314 90-91 -0.8608532314203856 2314 92-93 -1.3960477114056538 2314 94-95 -0.5287946455892794 2314 96-97 -0.8365166198319791 2314 98-99 -0.9049262813911483 2314 100-101 -1.642202459060762 2315 1 -0.2082332537508691 2315 2 -0.37395313464259416 2315 3 -0.06196223939926426 2315 4 -0.10835225058590936 2315 5 -0.21115203214946376 2315 6 0.09115764351079747 2315 7 -0.16148287136201134 2315 8 -0.23830626301177205 2315 9 -0.01684332162730584 2315 10-11 -0.3341465249306026 2315 12-13 0.13321311803063196 2315 14-15 0.07698668251912011 2315 16-17 -0.4604052624806698 2315 18-19 -0.3240908252770822 2315 20-21 -0.45723799675641885 2315 22-23 -0.6341350635096177 2315 24-25 -0.09166921015027185 2315 26-27 -0.4972804102674502 2315 28-29 -0.529070439428807 2315 30-31 -0.42033476660083835 2315 32-33 -0.46546000119152353 2315 34-35 -0.36139107464418174 2315 36-37 -0.1429687616962454 2315 38-39 -0.6546842673198583 2315 40-41 -0.2909291841455044 2315 42-43 -0.09409060693342752 2315 44-45 0.30889703060655194 2315 46-47 0.5699157073357171 2315 48-49 0.4160077558792423 2315 50-51 0.6390417580303165 2315 52-53 0.4531653953842607 2315 54-55 0.3009749673226807 2315 56-57 0.819772620844418 2315 58-59 0.8219442872661489 2315 60-61 1.2273076501759874 2315 62-63 0.849847421215312 2315 64-65 1.257023926318098 2315 66-67 0.27865902967523226 2315 68-69 0.27093340410524647 2315 70-71 0.4102610966663107 2315 72-73 0.10157283463120947 2315 74-75 -0.5674137828099255 2315 76-77 0.0016444773727108952 2315 78-79 -0.2083218685812085 2315 80-81 -0.21554631102377542 2315 82-83 0.44804857279287447 2315 84-85 -0.04901291626304527 2315 86-87 0.5850386484350913 2315 88-89 0.022407024726287972 2315 90-91 0.19269414172734756 2315 92-93 0.4986536806015316 2315 94-95 0.5492999510279937 2315 96-97 0.03651885389950138 2315 98-99 -0.3645131695815387 2315 100-101 0.16141975318291202 2316 1 -0.3958453240413533 2316 2 -0.3593007446635994 2316 3 -0.3818556765630525 2316 4 0.12121893646260418 2316 5 0.06186400350809862 2316 6 -0.04342916933821783 2316 7 -0.1381210188080928 2316 8 -0.3686408088389115 2316 9 -0.45759336005140483 2316 10-11 -0.0437627962559759 2316 12-13 0.05785065069111539 2316 14-15 -0.4715301472364928 2316 16-17 -0.5218069736339075 2316 18-19 -0.20602637525401946 2316 20-21 -0.14958903933032275 2316 22-23 0.34916326119964225 2316 24-25 -0.037773006451303104 2316 26-27 -0.41402819447770867 2316 28-29 -0.5604757368313358 2316 30-31 0.20935937756637912 2316 32-33 -0.2976747662921966 2316 34-35 -1.2314920019457887 2316 36-37 -1.0281319360391876 2316 38-39 -1.0881849589536472 2316 40-41 -1.3466185124922418 2316 42-43 -0.6844128570487058 2316 44-45 -0.9036650883544581 2316 46-47 -1.2211340390652197 2316 48-49 -0.7629460839691049 2316 50-51 -0.38985316486532184 2316 52-53 -0.30350758991211535 2316 54-55 -0.716648886358346 2316 56-57 -0.5551697430061893 2316 58-59 -0.7484486631923772 2316 60-61 -0.818288948010391 2316 62-63 -0.17228486015156363 2316 64-65 -0.07534453518095674 2316 66-67 -0.29946997283514065 2316 68-69 -0.150860745185188 2316 70-71 0.12202911756902068 2316 72-73 0.1451969957050352 2316 74-75 -0.19972076574962472 2316 76-77 0.28214040441815413 2316 78-79 0.15675830970661409 2316 80-81 0.5780138098840588 2316 82-83 0.8827276034809195 2316 84-85 0.6515608836242457 2316 86-87 1.1917039489115524 2316 88-89 0.8021857019126202 2316 90-91 0.7341921692902353 2316 92-93 1.09566172404638 2316 94-95 1.324903209391639 2316 96-97 0.5597526261214405 2316 98-99 0.9456461623514478 2316 100-101 0.7650879738909424 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 515.0 3 94.0 4 81.0 5 82.0 6 86.0 7 117.0 8 132.0 9 187.0 10 241.0 11 361.0 12 593.0 13 830.0 14 971.0 15 1269.0 16 1901.0 17 2542.0 18 2153.0 19 1174.0 20 1167.0 21 1076.0 22 1154.0 23 1255.0 24 1424.0 25 1407.0 26 1582.0 27 1836.0 28 2000.0 29 2406.0 30 2766.0 31 3486.0 32 4439.0 33 5822.0 34 8002.0 35 11756.0 36 16414.0 37 22630.0 38 18392.0 39 2416.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 45.14117665964935 19.47546884792775 15.313288443923057 20.070066048499847 2 14.986102408380328 20.6937549203901 42.082389421763786 22.237753249465786 3 19.20762810574598 26.819667917131913 31.308490043137034 22.664213933985074 4 11.573653810621702 17.134341787100038 38.802708847133296 32.489295555144956 5 11.822220793932626 38.57104981963381 34.54514778780259 15.061581598630985 6 28.551933404509803 37.35079029966365 18.346672232345693 15.75060406348085 7 25.703207783450534 35.8732299393122 21.35552130494814 17.06804097228912 8 25.924825792363766 33.97048906586173 20.368645836678333 19.736039305096178 9 26.458216263947982 16.756843541783738 21.810227181504374 34.97471301276391 10-11 24.033413079430325 27.71230391291084 27.079285817504534 21.1749971901543 12-13 25.792403681360803 25.56241897353771 28.827158263996754 19.81801908110473 14-15 22.294335805799058 26.048389686285844 29.024178788170694 22.633095719744407 16-17 19.220687468137463 30.00959388560395 29.50822304379067 21.261495602467917 18-19 19.171071802714128 26.870326441653518 34.35163220826761 19.606969547364745 20-21 20.44759466033056 25.771611132070927 34.52162116602583 19.259173041572684 22-23 19.751882354829906 25.773411035656373 35.05795565830243 19.416750951211288 24-25 19.480780114231585 25.960191536587423 35.1512986517783 19.407729697402694 26-27 19.04295581607221 26.285604202871216 35.27610439752291 19.395335583533672 28-29 18.66435469498832 26.90516902279058 35.32540198604789 19.105074296173203 30-31 18.9084501388822 26.325401795032356 35.11190854647336 19.654239519612094 32-33 18.71683298677735 26.654436853218154 34.88547595918401 19.743254200820495 34-35 19.260457517651638 26.535060431025144 34.64658587788558 19.557896173437644 36-37 19.204327916748603 26.231975374538973 34.993759355949486 19.56993735276294 38-39 19.010144950800196 26.85241664927685 34.67181405204103 19.46562434788192 40-41 18.8693708336845 26.891973785071293 34.6753835719245 19.563271809319705 42-43 18.793799619500213 26.988593033803472 33.917462050364044 20.30014529633227 44-45 19.0100623326791 27.0374517854921 33.69055216398351 20.261933717845288 46-47 19.46032077319505 27.368070383868222 33.02494902628157 20.14665981665516 48-49 19.5467899629148 27.40331358666859 32.76461333461767 20.28528311579894 50-51 19.4524385789573 27.77744329516218 32.39571166528191 20.374406460598617 52-53 19.690163348708747 28.239776153256336 31.572186158916736 20.497874339118184 54-55 19.35962884479548 27.73389841392153 32.01775508893598 20.88871765234701 56-57 19.419630316309906 27.796104114998432 31.7469681282255 21.03729744046616 58-59 19.753106238160978 27.321652165537614 32.021944328506756 20.903297267794652 60-61 19.456160134194253 27.14704784683235 32.8141867081877 20.582605310785702 62-63 19.126334376755757 27.027851352435988 33.01187896299864 20.833935307809615 64-65 19.06078567501515 26.967297651964188 33.14217836403986 20.829738308980804 66-67 19.138502532163695 26.94687673057939 32.73794714158447 21.176673595672447 68-69 18.962133525976764 26.81476130275119 32.598077579114246 21.625027592157807 70-71 19.233563020960837 26.80201782654236 32.31331693280734 21.65110221968946 72-73 19.019126275151894 26.606631191158414 32.670776046808406 21.703466486881283 74-75 18.872800821882624 26.997319250032103 32.12967124695004 22.000208681135224 76-77 18.832320670364158 26.514379279069583 32.260873752899535 22.392426297666727 78-79 19.213218443854924 26.562907608150354 32.04075981169256 22.18311413630216 80-81 18.909298135075588 26.388259042696216 31.967782196734074 22.734660625494122 82-83 18.886293772523334 26.01028950262053 32.14787347603798 22.955543248818152 84-85 18.835450149481332 25.815124701538956 32.23058247557134 23.118842673408373 86-87 18.84204528126229 25.598118764998112 32.19528246615141 23.364553487588182 88-89 18.690658683595913 25.459682014815765 32.1440323603297 23.70562694125862 90-91 18.691986339576303 25.692351527166345 32.51935293574705 23.096309197510305 92-93 18.60641495351105 25.976027191120348 32.27179884349456 23.145759011874045 94-95 18.272294591188913 26.38912298443733 31.510197202090005 23.828385222283757 96-97 18.327815566122915 26.550693232207706 31.48217259575834 23.639318605911033 98-99 18.850524896465377 26.69026292978908 30.601303412629623 23.857908761115926 100-101 19.564318932344644 25.705102740962765 30.75568074665254 23.974897580040043 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 180.0 1 127.5 2 91.5 3 122.5 4 108.5 5 88.0 6 164.5 7 360.5 8 617.5 9 728.0 10 587.5 11 443.0 12 414.0 13 416.5 14 447.5 15 494.5 16 500.5 17 506.0 18 534.0 19 567.0 20 611.0 21 651.0 22 747.0 23 864.0 24 977.5 25 1102.0 26 1300.5 27 1530.5 28 1676.0 29 1903.5 30 2137.5 31 2258.0 32 2431.0 33 2669.0 34 2990.0 35 3174.0 36 3331.0 37 3598.0 38 3675.5 39 3640.5 40 3644.5 41 3827.0 42 3999.0 43 4094.0 44 4235.5 45 4284.0 46 4264.5 47 4504.5 48 5077.5 49 4928.0 50 4210.0 51 3716.5 52 3433.5 53 3289.5 54 3056.5 55 2722.0 56 2294.5 57 1946.5 58 1739.5 59 1488.5 60 1212.5 61 1016.0 62 809.5 63 615.0 64 440.0 65 304.5 66 229.0 67 187.0 68 141.5 69 93.0 70 69.0 71 50.0 72 35.5 73 26.5 74 25.5 75 19.0 76 13.0 77 10.0 78 8.0 79 8.0 80 3.0 81 2.0 82 3.0 83 1.5 84 1.0 85 1.0 86 0.0 87 0.0 88 1.0 89 1.0 90 0.0 91 0.5 92 0.5 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.24447134074495627 2 0.2220280701191898 3 0.21802034322173147 4 0.22282961549868144 5 0.23244816005258137 6 0.14908744058544876 7 0.1498889859649404 8 0.15630134900087367 9 0.15149207672392373 10-11 0.15790443975985702 12-13 0.14868666789570292 14-15 0.1498889859649404 16-17 0.1607098485880778 18-19 0.1510913040341779 20-21 0.1458812590674821 22-23 0.14507971368799044 24-25 0.15028975865468622 26-27 0.14227430485976963 28-29 0.15229362210341538 30-31 0.15469825824189037 32-33 0.160309075898332 34-35 0.1567021216906195 36-37 0.13826657796231134 38-39 0.1322549876161239 40-41 0.139068123341803 42-43 0.14908744058544876 44-45 0.14868666789570292 46-47 0.14828589520595709 48-49 0.14507971368799044 50-51 0.1510913040341779 52-53 0.16792375700350276 54-55 0.14027044141104048 56-57 0.13305653299561554 58-59 0.13706425989307383 60-61 0.13105266954688638 62-63 0.13546116913409054 64-65 0.1326557603058697 66-67 0.12985035147764892 68-69 0.14147275948027796 70-71 0.13586194182383635 72-73 0.13305653299561554 74-75 0.13385807837510721 76-77 0.13626271451358218 78-79 0.1406712141007863 80-81 0.13586194182383635 82-83 0.13305653299561554 84-85 0.12944957878790309 86-87 0.12824726071866557 88-89 0.12985035147764892 90-91 0.1326557603058697 92-93 0.12784648802891976 94-95 0.13305653299561554 96-97 0.12944957878790309 98-99 0.13065189685714057 100-101 0.11983103423400315 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 124759.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 74.03313588598819 #Duplication Level Percentage of deduplicated Percentage of total 1 88.78013923324275 65.7267211183161 2 6.8479802518324435 10.139549050569498 3 1.8524733930253456 4.1143324329306905 4 0.876974546084471 2.5970070295529784 5 0.44714875004060073 1.6551912086502776 6 0.3118131719411453 1.3850704157615885 7 0.1981312863376027 1.0267796311288162 8 0.11584725485313382 0.6861228448448609 9 0.08986282385803838 0.59875439848027 >10 0.4352392191678486 5.301421139957839 >50 0.02381906174550415 1.1333851666012071 >100 0.01732295399673029 2.427880954480238 >500 0.0010826846247956432 0.7302078407169021 >1k 0.0021653692495912863 2.477576768008721 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1760 1.4107198679053214 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1331 1.0668569001033994 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 911 0.7302078407169021 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 315 0.2524867945398729 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 287 0.2300435239141064 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 286 0.22924197853461475 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 274 0.21962343398071482 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 239 0.19156934569850673 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 238 0.19076780031901505 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 218 0.17473689272918186 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 160 0.12824726071866557 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 149 0.11943026154425734 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 139 0.11141480774934072 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 133 0.10660553547239078 No Hit GTCCCAGGGTGTGCTTGTCAAAGAGATATTCTGCCATGCCAGCTTCAGGG 131 0.10500244471340744 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 8.0154537949166E-4 0.0 24-25 0.0 0.0 0.0 0.00160309075898332 0.0 26-27 0.0 0.0 0.0 0.00320618151796664 0.0 28-29 0.0 0.0 0.0 0.0076146811051707695 0.0 30-31 0.0 0.0 0.0 0.019237089107799837 0.0 32-33 0.0 0.0 0.0 0.028054088282208095 0.0 34-35 0.0 0.0 0.0 0.034466451318141376 0.0 36-37 0.0 0.0 0.0 0.044886541251532956 0.0 38-39 0.0 0.0 0.0 0.06893290263628275 0.0 40-41 0.0 0.0 0.0 0.1282472607186656 0.0 42-43 0.0 0.0 0.0 0.23845975039876882 0.0 44-45 0.0 0.0 0.0 0.3546838304250595 0.0 46-47 0.0 0.0 0.0 0.5899373993058616 0.0 48-49 0.0 0.0 0.0 0.8247901954969181 0.0 50-51 0.0 0.0 0.0 0.9794884537388084 0.0 52-53 0.0 0.0 0.0 1.1578323006757028 0.0 54-55 0.0 0.0 0.0 1.362226372446076 0.0 56-57 0.0 0.0 0.0 1.8343366009666635 0.0 58-59 0.0 0.0 0.0 2.3890060035748926 0.0 60-61 0.0 0.0 0.0 2.955698586875496 0.0 62-63 0.0 0.0 0.0 3.580903982878991 0.0 64-65 0.0 0.0 0.0 4.212922514608165 0.0 66-67 0.0 0.0 0.0 4.949542718361 0.0 68-69 0.0 0.0 0.0 5.716220873844772 0.0 70-71 0.0 0.0 0.0 6.571469793762374 0.0 72-73 0.0 0.0 0.0 7.405878533813192 0.0 74-75 0.0 0.0 0.0 8.263131317179521 0.0 76-77 0.0 0.0 0.0 9.33199208073165 0.0 78-79 0.0 0.0 0.0 10.28743417308571 0.0 80-81 0.0 0.0 0.0 11.406792295545813 0.0 82-83 0.0 0.0 0.0 12.830737662212748 0.0 84-85 0.0 0.0 0.0 14.445450829198695 0.0 86-87 0.0 0.0 0.0 15.827315063442317 0.0 88-89 0.0 0.0 0.0 17.287329972186377 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 695 0.0 44.444164 1 GTATCAA 1200 0.0 38.808872 1 GTGCAGA 65 1.3179558E-4 36.55467 1 GTCCTAT 55 0.0025652607 34.560776 1 CTGGACA 55 0.0025652607 34.560776 4 GTGTGGG 60 0.0039290255 31.680712 1 TAAGGTG 75 3.0548804E-4 31.680712 5 GACGTAT 30 0.0039520096 31.655308 62-63 ATCAACG 1500 0.0 31.047096 3 TCAACGC 1485 0.0 31.040699 4 TATCAAC 1535 0.0 30.648766 2 CAACGCA 1500 0.0 30.413483 5 GTACATG 820 0.0 30.13531 1 AACGCAG 1515 0.0 30.10028 6 CATGGGG 190 1.0913936E-11 30.013306 4 TTCCTAC 80 4.4593378E-4 29.700668 1 TACATGG 845 0.0 29.243734 2 AGAGCCC 65 0.0058117495 29.243734 5 ACATGGG 870 0.0 28.403397 3 CCTACCC 70 0.008345318 27.154896 3 >>END_MODULE