FastQCFastQC Report
Thu 26 May 2016
SRR937695_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937695_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences124759
Sequences flagged as poor quality0
Sequence length101
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13841.1093388052164574No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12160.9746791814618584No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8430.6757027549114694No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT6850.549058584951787No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA6160.49375195376686254No Hit
GTCCCAGGGTGTGCTTGTCAAAGAGATATTCTGCCATGCCAGCTTCAGGG3130.2508837037808896No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2560.20519561714986492No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2240.17954616500613182No Hit
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA1940.15549980362138202No Hit
CTACTGGAACTGCACAAACTGGCTACTGACAAGAATGATCCCCACTTATG1880.15069053134443205No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT1620.12985035147764892No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1370.10981171699035741No Hit
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT1360.10901017161086574No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT1260.10099471781594915No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1250.1001931724364575No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAAGGTG502.8060986E-547.5180445
GTAAGGT601.4776488E-647.5180444
CTACGTC500.001607294438.0144353
CGTCTAT500.001610465537.99926
AAGGTGT500.001610465537.99926
AGGTAAG651.3184556E-436.552342
CCTACGT550.002566069134.558582
TCCTACG550.002566069134.558581
GGTAAGG600.003930261431.6786983
ACGTCTA600.003930261431.6786985
GGTTCAC300.003945484331.66599884-85
AGGGTGT953.466717E-529.9993676
TTGTAGG650.00581357629.2418731
ACAGTAC650.005824984529.2301528
GTCTATC650.005824984529.2301527
TACATAA700.00834793327.1531682
CTTCTAT700.00834793327.1531681
TCCGCCC350.00838006627.14228488-89
GTATATA908.882991E-426.3989121
GTTATAG908.882991E-426.3989121
Produced by FastQC (version 0.11.5)