##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937695_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 124759 Sequences flagged as poor quality 0 Sequence length 101 %GC 41 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.18677610432915 33.0 31.0 34.0 31.0 34.0 2 32.49198855393198 34.0 31.0 34.0 31.0 34.0 3 32.56418374626279 34.0 31.0 34.0 31.0 34.0 4 36.18894027685377 37.0 35.0 37.0 35.0 37.0 5 36.133793954744746 37.0 35.0 37.0 35.0 37.0 6 36.20247036285959 37.0 36.0 37.0 35.0 37.0 7 36.15246194663311 37.0 36.0 37.0 35.0 37.0 8 36.141633068556175 37.0 36.0 37.0 35.0 37.0 9 37.8205580358932 39.0 38.0 39.0 35.0 39.0 10-11 37.748984041231495 39.0 37.0 39.0 35.0 39.0 12-13 37.66120279899647 39.0 37.0 39.0 35.0 39.0 14-15 38.811552673554615 40.0 38.0 41.0 35.0 41.0 16-17 38.797850255292204 40.0 38.0 41.0 35.5 41.0 18-19 38.84235606248848 40.0 38.0 41.0 35.0 41.0 20-21 38.797373335791406 40.0 38.0 41.0 35.0 41.0 22-23 38.722008031484705 40.0 38.0 41.0 35.0 41.0 24-25 38.70322782324321 40.0 38.0 41.0 35.0 41.0 26-27 38.523970214573694 40.0 38.0 41.0 34.0 41.0 28-29 38.35192651431961 40.0 38.0 41.0 34.0 41.0 30-31 38.308687148822926 40.0 37.5 41.0 34.0 41.0 32-33 38.13454339967457 40.0 37.0 41.0 34.0 41.0 34-35 37.90852363356551 40.0 37.0 41.0 33.5 41.0 36-37 37.70134819932831 40.0 36.0 41.0 33.0 41.0 38-39 37.510319896760954 40.0 36.0 41.0 33.0 41.0 40-41 37.255997563302046 40.0 36.0 41.0 33.0 41.0 42-43 36.932726296299265 40.0 35.5 41.0 32.5 41.0 44-45 36.450528619177774 40.0 35.0 41.0 31.0 41.0 46-47 35.81824557747337 39.0 35.0 41.0 29.0 41.0 48-49 35.26800070535994 39.0 35.0 41.0 26.5 41.0 50-51 34.86238668152197 39.0 35.0 40.5 24.0 41.0 52-53 34.599716252935664 39.0 35.0 40.0 23.0 41.0 54-55 34.34624756530591 38.5 34.5 40.0 21.5 41.0 56-57 34.01679638342725 38.0 34.0 40.0 19.5 41.0 58-59 33.73496902027108 38.0 34.0 40.0 17.5 41.0 60-61 33.363260365985624 37.5 33.5 40.0 12.0 41.0 62-63 32.99844099423689 37.0 33.0 40.0 7.5 41.0 64-65 32.57630712012761 36.5 33.0 39.5 4.0 41.0 66-67 32.132102693994014 36.0 32.0 39.0 2.0 40.5 68-69 31.64149279811477 35.5 31.5 39.0 2.0 40.0 70-71 31.201576639761463 35.0 31.0 38.5 2.0 40.0 72-73 30.68397871095472 35.0 31.0 37.5 2.0 40.0 74-75 30.248984041231495 35.0 30.0 37.0 2.0 39.0 76-77 29.055490986622207 33.5 28.5 35.5 2.0 38.5 78-79 29.547519617823163 34.0 30.0 36.0 2.0 39.0 80-81 29.325423416346716 34.0 30.0 36.0 2.0 37.5 82-83 28.95220384902091 34.0 29.0 35.0 2.0 37.0 84-85 28.57154193284653 34.0 29.0 35.0 2.0 37.0 86-87 28.22428442036246 34.0 29.0 35.0 2.0 36.0 88-89 27.80804190479244 34.0 28.0 35.0 2.0 36.0 90-91 27.318129353393342 33.5 26.5 35.0 2.0 35.0 92-93 26.910222909770035 33.0 25.5 35.0 2.0 35.0 94-95 26.46835498841767 33.0 25.0 35.0 2.0 35.0 96-97 25.954660585609055 33.0 24.5 35.0 2.0 35.0 98-99 25.112653195360657 32.0 19.0 34.0 2.0 35.0 100-101 22.70357649548329 29.5 2.0 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.3215584940315992 1101 2 0.3618827169042049 1101 3 0.44696139921887834 1101 4 0.26560685787798377 1101 5 0.11558064534731471 1101 6 0.27598974849450286 1101 7 0.39037551570538653 1101 8 0.11885318258325128 1101 9 0.2896141291371208 1101 10-11 0.1837447059185493 1101 12-13 0.2077916053629849 1101 14-15 0.5653857604704697 1101 16-17 0.18636512443702458 1101 18-19 0.39933353332040866 1101 20-21 0.13222280459885383 1101 22-23 0.28820241688494974 1101 24-25 0.23964321860143656 1101 26-27 0.1925097257964481 1101 28-29 0.09905621299596845 1101 30-31 0.27951929695542077 1101 32-33 0.21473286295606187 1101 34-35 0.02339801329675595 1101 36-37 0.20578104616599546 1101 38-39 0.25101050621559295 1101 40-41 0.3212111361165171 1101 42-43 0.4771721868453227 1101 44-45 0.39257239355506357 1101 46-47 0.4283575520424421 1101 48-49 0.06735925705648071 1101 50-51 -0.10403337018801295 1101 52-53 0.21103536217479046 1101 54-55 0.02774968041310899 1101 56-57 -0.11241379496020443 1101 58-59 -0.2515827106337696 1101 60-61 0.14434807954833673 1101 62-63 0.20986344940077117 1101 64-65 0.18713461039920176 1101 66-67 0.6696945724479129 1101 68-69 0.7345327420089589 1101 70-71 0.5695037068155635 1101 72-73 0.3983572031925924 1101 74-75 1.025136628238215 1101 76-77 0.7445762049024189 1101 78-79 0.9152592333384781 1101 80-81 0.7311285318004153 1101 82-83 0.6393377890552081 1101 84-85 1.1646191306336817 1101 86-87 0.8128910771545037 1101 88-89 0.6389262973473784 1101 90-91 0.6428000659253819 1101 92-93 -0.0053415401766976345 1101 94-95 -0.8860030317927965 1101 96-97 -0.40705027592194654 1101 98-99 -0.07333329452676196 1101 100-101 -0.3557459545469186 1102 1 0.2898511769584289 1102 2 0.34237052178225724 1102 3 0.08291049784030236 1102 4 0.2271126903276155 1102 5 0.009324017563642428 1102 6 0.06930894255600606 1102 7 0.14668516575841295 1102 8 0.34907587611453295 1102 9 0.20106693931739272 1102 10-11 0.2331614609556638 1102 12-13 0.34792946326330565 1102 14-15 0.32917155050228786 1102 16-17 0.31510319442642043 1102 18-19 0.09180437107226425 1102 20-21 0.31117296366566194 1102 22-23 0.1894749513494176 1102 24-25 0.1958468241157476 1102 26-27 0.31965712770524846 1102 28-29 0.25939555551983773 1102 30-31 0.5038565185884991 1102 32-33 0.2600138809836281 1102 34-35 0.2316164650464927 1102 36-37 0.40424340035475126 1102 38-39 0.5628344722813381 1102 40-41 0.5303309664452556 1102 42-43 0.692654689496436 1102 44-45 1.0233253097798851 1102 46-47 0.860064869115611 1102 48-49 0.8344854500575423 1102 50-51 0.7618202883485665 1102 52-53 1.2088084268619568 1102 54-55 0.7323626178468317 1102 56-57 0.6697177002677961 1102 58-59 0.845766175898568 1102 60-61 0.5270628197392213 1102 62-63 0.7944869912883661 1102 64-65 0.8178345043546607 1102 66-67 1.7524093126387932 1102 68-69 1.7476292425391797 1102 70-71 1.2114443218738913 1102 72-73 1.2456530674555815 1102 74-75 0.9527612305711983 1102 76-77 0.5110661306712387 1102 78-79 1.3931489470182257 1102 80-81 1.45493553073997 1102 82-83 1.5844597402747205 1102 84-85 1.7004621847163932 1102 86-87 1.8098688078013758 1102 88-89 1.193698301589162 1102 90-91 1.0448679344301546 1102 92-93 0.9940752148604126 1102 94-95 1.1040818038381666 1102 96-97 0.8445403921586454 1102 98-99 0.6455426333629539 1102 100-101 0.5084640136397205 1103 1 0.6426967054137123 1103 2 0.46432174129445514 1103 3 0.3170568393037172 1103 4 0.17833220252273918 1103 5 0.3052589769132368 1103 6 0.33922764174300113 1103 7 0.006847767384428494 1103 8 0.1962303476592524 1103 9 0.19131084175641888 1103 10-11 0.5177143064841232 1103 12-13 0.49996198358850563 1103 14-15 0.3429926887136645 1103 16-17 0.3882739261337349 1103 18-19 0.5462759157877102 1103 20-21 0.33393719130793187 1103 22-23 0.27646682126812294 1103 24-25 0.17145658021331656 1103 26-27 -0.15270059587198404 1103 28-29 0.16671262869056847 1103 30-31 0.20548253484866308 1103 32-33 0.007168352528346134 1103 34-35 0.4348684975668178 1103 36-37 0.3879832377531187 1103 38-39 -0.2062712187755693 1103 40-41 -0.009506431928734571 1103 42-43 0.38452460819561907 1103 44-45 0.1582846593733791 1103 46-47 0.14705673903431205 1103 48-49 0.45237162891933735 1103 50-51 0.3585682558282457 1103 52-53 0.4608409471871582 1103 54-55 0.5535008292289376 1103 56-57 0.5778477815686074 1103 58-59 0.5368230864676775 1103 60-61 0.7904774538855577 1103 62-63 0.7359504059225088 1103 64-65 0.9324686506961193 1103 66-67 0.8011898004436695 1103 68-69 1.171206478311536 1103 70-71 1.2935581430121026 1103 72-73 1.0058156690815956 1103 74-75 0.8641433443923319 1103 76-77 0.9679766997769335 1103 78-79 1.2199782153109062 1103 80-81 1.2329843112277743 1103 82-83 0.9316142118194328 1103 84-85 1.1500556806513558 1103 86-87 0.6846655557688557 1103 88-89 1.0327226918330652 1103 90-91 1.4708841945927524 1103 92-93 1.5802540766490338 1103 94-95 1.598390746927599 1103 96-97 1.704702993784661 1103 98-99 1.8983881618182359 1103 100-101 2.142610355103134 1104 1 0.1302004693402381 1104 2 0.32731481566963794 1104 3 0.21588249422693195 1104 4 0.2810121182107821 1104 5 0.3389837586055009 1104 6 0.15645136953583005 1104 7 0.17727836057924407 1104 8 -0.04255315128685311 1104 9 0.07989861652305308 1104 10-11 0.4975396602932136 1104 12-13 0.22730314588902445 1104 14-15 -0.10506512519780387 1104 16-17 0.4635524566968172 1104 18-19 0.47415908350827607 1104 20-21 -0.16726726518107427 1104 22-23 -0.08050397066201498 1104 24-25 0.2182798262235508 1104 26-27 -0.3419508217075773 1104 28-29 -0.007331333971286824 1104 30-31 -0.010416290806254835 1104 32-33 -0.15558383054904112 1104 34-35 0.311562264504488 1104 36-37 -0.2825979125028226 1104 38-39 -0.14890897848650297 1104 40-41 -0.9024603614680231 1104 42-43 -0.5097843348938085 1104 44-45 -0.18799658121680096 1104 46-47 -0.3950992380810163 1104 48-49 -0.4729520687620834 1104 50-51 -0.9027988022867817 1104 52-53 -0.4304568546797469 1104 54-55 -0.2441504806174919 1104 56-57 0.3225030058986249 1104 58-59 -0.20391765427306296 1104 60-61 -0.14132019742428525 1104 62-63 -0.6097286064231682 1104 64-65 -0.8290489644800267 1104 66-67 -0.8270848156358248 1104 68-69 -0.7464388092524494 1104 70-71 -0.7815698304898575 1104 72-73 -0.505024439319488 1104 74-75 -0.6542246170650614 1104 76-77 -1.0428634086241502 1104 78-79 -0.8422018509341989 1104 80-81 -0.88038515580023 1104 82-83 -0.4637486306978822 1104 84-85 -0.3216395918569255 1104 86-87 -0.2245485363720654 1104 88-89 0.004116850219091361 1104 90-91 -0.02008238173967314 1104 92-93 0.5325274882720414 1104 94-95 -0.23976041236177537 1104 96-97 0.3817580976690387 1104 98-99 0.6894149609751175 1104 100-101 0.05378168903869707 1105 1 0.5851948576679646 1105 2 0.5073372623587531 1105 3 0.4576333359777749 1105 4 0.2976226681546663 1105 5 0.12506681135521802 1105 6 0.30168144809924513 1105 7 0.12318183981606268 1105 8 0.10739073199036397 1105 9 0.5066101765679463 1105 10-11 0.32761822370510885 1105 12-13 0.4277520796712935 1105 14-15 0.9205241003914182 1105 16-17 0.44525840506943837 1105 18-19 0.411981754664275 1105 20-21 -0.18062304520351802 1105 22-23 -0.13314145663283483 1105 24-25 0.2060463806567725 1105 26-27 0.7099010301442732 1105 28-29 0.40063724066396134 1105 30-31 0.8144995341095651 1105 32-33 0.9143376060390622 1105 34-35 0.4650976180398345 1105 36-37 0.6202079236363431 1105 38-39 1.2049630753855496 1105 40-41 0.9682467092390468 1105 42-43 0.3902156651061972 1105 44-45 -0.014146974033863557 1105 46-47 0.31926664295153273 1105 48-49 0.5460153835387018 1105 50-51 0.6414211752665295 1105 52-53 0.8802053226490969 1105 54-55 0.3390145025474922 1105 56-57 0.6850170350793263 1105 58-59 0.6792473288919254 1105 60-61 0.520632664528577 1105 62-63 0.46322313319523545 1105 64-65 -0.13914997458103784 1105 66-67 -0.10856629711730648 1105 68-69 -0.36922219870250217 1105 70-71 -0.5271366093899665 1105 72-73 -1.2312870655821122 1105 74-75 -1.4268396563467647 1105 76-77 -0.8024593682201129 1105 78-79 -1.7888909642899797 1105 80-81 -1.4386343140493878 1105 82-83 -1.6288440291266113 1105 84-85 -1.2729302764809418 1105 86-87 -1.638689950513477 1105 88-89 -1.2865677737988648 1105 90-91 -0.6850655467228428 1105 92-93 -0.029649840571956076 1105 94-95 -0.8083350476030731 1105 96-97 -1.3791846632737261 1105 98-99 -0.726297721404233 1105 100-101 0.5377323063226491 1106 1 0.1805328530059569 1106 2 0.638805793827288 1106 3 0.5634608418052807 1106 4 0.5535667240987223 1106 5 0.3427824290708372 1106 6 0.5030800494978465 1106 7 0.43273894937316726 1106 8 -0.23153700693737278 1106 9 0.5746754446332147 1106 10-11 0.4467324428193322 1106 12-13 0.700712452631663 1106 14-15 0.840803820671141 1106 16-17 0.5343026941137268 1106 18-19 0.4133803560628806 1106 20-21 0.9443186797382097 1106 22-23 0.5628958394044545 1106 24-25 1.2633890379994313 1106 26-27 0.817593337836577 1106 28-29 0.7386325786592991 1106 30-31 0.44969766930770305 1106 32-33 0.7541977458992051 1106 34-35 1.0765195294617484 1106 36-37 1.5896717931002158 1106 38-39 1.4550796255021012 1106 40-41 1.614633655625994 1106 42-43 -0.4072202323297063 1106 44-45 0.7583471984603065 1106 46-47 1.2283575520424392 1106 48-49 1.9676937052170231 1106 50-51 1.0292999631453128 1106 52-53 1.386732129175904 1106 54-55 1.240180003712993 1106 56-57 1.645389995452284 1106 58-59 1.8701564198010132 1106 60-61 1.484035927931835 1106 62-63 0.4667196366917352 1106 64-65 -0.07691221234327728 1106 66-67 0.42220293365192774 1106 68-69 1.05175682227652 1106 70-71 1.8276419453885886 1106 72-73 1.6915567572617114 1106 74-75 1.8775892480821383 1106 76-77 1.2178203520596114 1106 78-79 1.3975892221902093 1106 80-81 1.59539831998325 1106 82-83 1.8277993275167468 1106 84-85 1.267629164078496 1106 86-87 2.113058301234773 1106 88-89 2.2472317600006697 1106 90-91 1.6622538005965062 1106 92-93 2.1640564531343394 1106 94-95 2.168121828853799 1106 96-97 2.1837523996633372 1106 98-99 -0.04657744168395439 1106 100-101 -0.7391907706004304 1107 1 0.2739394464125482 1107 2 0.32378747880896697 1107 3 -0.24972597138152963 1107 4 -0.21016953963754048 1107 5 0.2640278503162534 1107 6 0.33458188099967856 1107 7 0.32101733765155416 1107 8 -0.24802052342089098 1107 9 0.45379632375409784 1107 10-11 0.7709082669951499 1107 12-13 0.5734230753422835 1107 14-15 1.052342282209601 1107 16-17 0.6240462838573109 1107 18-19 0.030596473278997394 1107 20-21 0.3838791192986477 1107 22-23 0.46491049141911134 1107 24-25 0.5216307962411832 1107 26-27 0.5218057920490295 1107 28-29 -0.4500121099853871 1107 30-31 0.12185884146887105 1107 32-33 0.13057137227283278 1107 34-35 0.42267337561559515 1107 36-37 -0.12827692484850672 1107 38-39 -0.8168983964759207 1107 40-41 -0.4064286154362762 1107 42-43 -1.1645462396557136 1107 44-45 -0.4357919956788834 1107 46-47 -0.8430710193861302 1107 48-49 -0.9297421922188818 1107 50-51 -0.661176227330877 1107 52-53 0.41786766031143685 1107 54-55 0.7942093077422996 1107 56-57 0.9869650870273716 1107 58-59 -1.2488911992466072 1107 60-61 -1.7696270757311652 1107 62-63 -2.2164305464584437 1107 64-65 -1.9679378533689196 1107 66-67 -2.47523296378397 1107 68-69 -2.178096657576962 1107 70-71 -2.4901236223769807 1107 72-73 -2.5566117409067886 1107 74-75 -2.3742422537493617 1107 76-77 -1.9277840435447864 1107 78-79 -2.2791507045497177 1107 80-81 -2.13170790712298 1107 82-83 -2.663043163325746 1107 84-85 -3.511674865225533 1107 86-87 -2.502326314149844 1107 88-89 -1.9835374707685638 1107 90-91 -1.927489789147085 1107 92-93 -1.8038922648143831 1107 94-95 -1.499460588728617 1107 96-97 -2.1486651827542467 1107 98-99 -1.793830188936699 1107 100-101 -0.7456010270106859 1108 1 0.1899795466631815 1108 2 0.24609324321999537 1108 3 -0.04045278842414035 1108 4 0.22968008442261123 1108 5 0.22267446685761172 1108 6 0.20550919929541323 1108 7 0.13179428000744764 1108 8 0.16050077983725686 1108 9 -0.17903575644640313 1108 10-11 0.002737840929988522 1108 12-13 0.5502401179488032 1108 14-15 0.3812896506306558 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0.9473598018956579 2316 64-65 1.118751184662404 2316 66-67 1.6041853807350428 2316 68-69 1.6335843906812677 2316 70-71 1.3073356297802583 2316 72-73 1.7317391699273443 2316 74-75 1.4515007951480499 2316 76-77 1.4276809612490773 2316 78-79 1.4176374066679251 2316 80-81 1.2447702008987527 2316 82-83 1.3430290642229927 2316 84-85 1.2712429999070949 2316 86-87 1.1352575784676056 2316 88-89 0.9437555941083993 2316 90-91 1.060205960293633 2316 92-93 0.5814544913377446 2316 94-95 0.6284556784493951 2316 96-97 0.6742015478306094 2316 98-99 0.918058593421879 2316 100-101 1.3947792861884203 >>END_MODULE >>Per sequence quality scores pass #Quality Count 4 2.0 5 8.0 6 9.0 7 14.0 8 35.0 9 76.0 10 136.0 11 250.0 12 379.0 13 569.0 14 719.0 15 1143.0 16 1697.0 17 2393.0 18 2206.0 19 1192.0 20 932.0 21 883.0 22 950.0 23 934.0 24 1086.0 25 1178.0 26 1267.0 27 1425.0 28 1780.0 29 2048.0 30 2455.0 31 3114.0 32 3935.0 33 5414.0 34 7464.0 35 11424.0 36 17650.0 37 26581.0 38 21437.0 39 1974.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.79123240227047 20.530417214507903 15.366545874354617 19.311804508867013 2 15.436160918250385 22.01925312001539 40.585448745180706 21.959137216553515 3 19.771719875920777 27.445715339173926 30.547695957806653 22.234868827098648 4 12.197917585104081 18.39386336857461 37.745573465641755 31.66264558067955 5 12.139483791303512 39.60967630715941 33.42367119157773 14.827168709959349 6 29.27804807669186 38.43009321972764 16.989555863705224 15.302302839875278 7 26.51672424434309 36.6362346604253 19.867103776080285 16.979937319151322 8 26.579244783943444 34.81512355822025 19.142506753019823 19.463124904816485 9 27.62526150418006 17.423993459389703 20.316770733975105 34.63397430245514 10-11 24.707636322830417 28.419192202566546 26.10873764618184 20.764433828421197 12-13 26.403433889216192 26.562944616112187 27.52241366181321 19.511207832858407 14-15 22.70337210141152 27.020094742663858 28.00960251364631 22.26693064227831 16-17 19.931628179129362 30.688367171907437 28.306975849437716 21.073028799525485 18-19 19.52524467172709 28.124624275603367 33.172356302952096 19.177774749717454 20-21 21.20127606024415 26.602088827258957 33.2825687926322 18.914066319864702 22-23 20.554994469292552 26.936549159172156 33.09807787877331 19.410378492761986 24-25 20.50553467084539 27.372774709640186 33.373544193204495 18.748146426309926 26-27 19.7258681842775 27.50095184658237 33.791154840390355 18.982025128749775 28-29 19.233636319915355 28.179355231728625 33.70605492232963 18.880953526026385 30-31 19.568928894909384 27.524667559053857 33.345890877612035 19.560512668424725 32-33 19.65589656858423 27.97032679005122 32.95153055090214 19.422246090462412 34-35 19.892352455534272 27.77034121786805 33.13348135204674 19.203824974550933 36-37 20.364863456744605 27.432089067722572 33.17796712060853 19.025080354924295 38-39 19.598586073950578 28.09136014235446 33.19159339205989 19.11846039163507 40-41 19.472019942528966 28.66979003434636 32.41622815279119 19.441961870333486 42-43 19.754377948526788 28.562782327076548 31.802604523646334 19.880235200750334 44-45 20.213772152710426 28.170713134924135 31.69069966896176 19.924815043403683 46-47 20.499523080499202 29.35980570540001 30.797778116208047 19.342893097892738 48-49 20.864226228167908 29.11974286424226 30.228280124079227 19.787750783510607 50-51 20.410551543375625 29.382248976025778 30.38818842728781 19.819011053310785 52-53 20.51715707884802 30.02909609727555 29.522920190126563 19.93082663374987 54-55 19.862854486285446 29.774844098174064 29.65781753474727 20.704483880793216 56-57 20.350034867224007 29.780617029633135 29.76819307625101 20.10115502689185 58-59 20.711531833374746 29.075257095680474 29.80185798218966 20.411353088755117 60-61 19.909585681193338 29.408299200859254 30.772930209443807 19.909184908503594 62-63 20.09313957309693 28.785097668304495 30.836252294423648 20.28551046417493 64-65 19.437074679983006 29.173045631978457 30.72203207784609 20.667847610192453 66-67 19.774525284748997 28.900119430261544 30.367348247421027 20.958007037568432 68-69 19.83464118821087 28.915348792471885 29.964571694226468 21.285438325090773 70-71 20.066287802883963 29.263219487171266 29.76498689473305 20.90550581521173 72-73 19.917200362298512 28.781891486786527 30.04713086831411 21.253777282600854 74-75 19.389783502593 29.5722152309653 29.159419360527096 21.878581905914604 76-77 19.33487764409782 28.668072042898707 29.769796167009993 22.227254145993474 78-79 19.70879856363068 28.82557570996882 29.482442148462233 21.983183577938263 80-81 19.45230404219335 28.717367083737443 29.322934617943396 22.50739425612581 82-83 18.98219767712149 28.619177774749716 29.192282721086254 23.206341827042536 84-85 18.654440378811866 28.429726231078444 29.555902002669153 23.359931387440536 86-87 18.693641340504495 28.465681834577065 29.150201588662945 23.6904752362555 88-89 18.033568720493108 28.474498833751472 29.0247597367725 24.46717270898292 90-91 18.357850014028617 28.42278247625155 29.091747164214997 24.12762034550483 92-93 18.34945113960171 28.60446382410818 29.01205128307891 24.034033753211204 94-95 17.89730600597953 28.97346083248503 28.328216801994245 24.801016359541194 96-97 17.85963337314342 29.687236992922355 28.21439735810643 24.238732275827797 98-99 18.336699850911366 29.9748312733452 26.9257282098142 24.76274066592924 100-101 17.625365775443942 29.14699162223915 26.927887120695875 26.299755481621034 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 286.0 1 182.0 2 99.0 3 123.5 4 111.0 5 117.5 6 181.0 7 317.5 8 456.0 9 504.5 10 492.5 11 449.5 12 452.0 13 509.0 14 527.0 15 529.0 16 557.0 17 561.0 18 553.0 19 570.0 20 572.0 21 591.0 22 684.5 23 784.5 24 896.0 25 1057.0 26 1237.0 27 1413.0 28 1555.5 29 1753.0 30 2055.0 31 2214.0 32 2318.5 33 2509.5 34 2761.5 35 2951.5 36 3230.5 37 3538.5 38 3641.5 39 3647.0 40 3644.5 41 3856.5 42 4039.5 43 4088.0 44 4183.5 45 4217.0 46 4134.0 47 4500.0 48 5156.0 49 4937.5 50 4241.0 51 3793.0 52 3630.5 53 3485.5 54 3132.5 55 2828.5 56 2481.5 57 2151.0 58 1968.0 59 1756.0 60 1406.5 61 1084.0 62 857.5 63 653.5 64 454.0 65 311.5 66 229.0 67 183.5 68 138.0 69 89.5 70 64.5 71 44.5 72 32.0 73 26.0 74 27.5 75 24.0 76 13.0 77 9.0 78 9.0 79 8.0 80 6.5 81 5.0 82 4.5 83 4.0 84 2.0 85 1.5 86 1.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.02164172524627482 2 0.0 3 0.0 4 0.0 5 0.03366490593864972 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0020038634487291496 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 8.015453794916599E-4 24-25 0.0 26-27 0.00120231806923749 28-29 8.015453794916599E-4 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 4.0077268974582993E-4 42-43 0.01162240800262907 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 8.015453794916599E-4 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 0.0 84-85 4.0077268974582993E-4 86-87 0.0 88-89 0.0 90-91 0.011221635312883239 92-93 4.0077268974582993E-4 94-95 0.0 96-97 0.0 98-99 8.015453794916599E-4 100-101 0.01923708910779984 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 124759.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 71.36479131766045 #Duplication Level Percentage of deduplicated Percentage of total 1 87.78331873216973 62.64638222492967 2 7.289350135903138 10.404059025801745 3 2.104813891322416 4.506288123502112 4 0.9254891389806141 2.641893570804511 5 0.482961565244738 1.7233225659070688 6 0.32234876564009257 1.3802611434846384 7 0.21003212255992096 1.0492229017545829 8 0.15499696745063682 0.8849060989587926 9 0.14151897028101623 0.9089524603435423 >10 0.534627221061617 6.687293101098919 >50 0.028079160770042907 1.430758502392613 >100 0.01684749646202574 1.9341290007133753 >500 0.0033694992924051485 1.7185132936301188 >1k 0.0022463328616034325 2.084017986678316 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1384 1.1093388052164574 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1216 0.9746791814618584 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 843 0.6757027549114694 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 685 0.549058584951787 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 616 0.49375195376686254 No Hit GTCCCAGGGTGTGCTTGTCAAAGAGATATTCTGCCATGCCAGCTTCAGGG 313 0.2508837037808896 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 256 0.20519561714986492 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 224 0.17954616500613182 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 194 0.15549980362138202 No Hit CTACTGGAACTGCACAAACTGGCTACTGACAAGAATGATCCCCACTTATG 188 0.15069053134443205 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 162 0.12985035147764892 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 137 0.10981171699035741 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 136 0.10901017161086574 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 126 0.10099471781594915 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 125 0.1001931724364575 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 4.0077268974583E-4 0.0 12-13 0.0 0.0 0.0 8.0154537949166E-4 0.0 14-15 0.0 0.0 0.0 8.0154537949166E-4 0.0 16-17 0.0 0.0 0.0 8.0154537949166E-4 0.0 18-19 0.0 0.0 0.0 8.0154537949166E-4 0.0 20-21 0.0 0.0 0.0 8.0154537949166E-4 0.0 22-23 0.0 0.0 0.0 0.00160309075898332 0.0 24-25 0.0 0.0 0.0 0.0024046361384749797 0.0 26-27 0.0 0.0 0.0 0.004809272276949959 0.0 28-29 0.0 0.0 0.0 0.009217771864154088 0.0 30-31 0.0 0.0 0.0 0.02164172524627482 0.0 32-33 0.0 0.0 0.0 0.03126026980017474 0.0 34-35 0.0 0.0 0.0 0.037672632836108014 0.0 36-37 0.0 0.0 0.0 0.048493495459245424 0.0 38-39 0.0 0.0 0.0 0.07253985684399522 0.0 40-41 0.0 0.0 0.0 0.13185421492637805 0.0 42-43 0.0 0.0 0.0 0.24046361384749798 0.0 44-45 0.0 0.0 0.0 0.3566876938737887 0.0 46-47 0.0 0.0 0.0 0.5947466715828116 0.0 48-49 0.0 0.0 0.0 0.8291986950841221 0.0 50-51 0.0 0.0 0.0 0.9867023621542335 0.0 52-53 0.0 0.0 0.0 1.1678516179193483 0.0 54-55 0.0 0.0 0.0 1.3710433716204844 0.0 56-57 0.0 0.0 0.0 1.845959008969293 0.0 58-59 0.0 0.0 0.0 2.4030330477159962 0.0 60-61 0.0 0.0 0.0 2.971729494465329 0.0 62-63 0.0 0.0 0.0 3.597736435848316 0.0 64-65 0.0 0.0 0.0 4.234965012544185 0.0 66-67 0.0 0.0 0.0 4.975592943194479 0.0 68-69 0.0 0.0 0.0 5.753091961301389 0.0 70-71 0.0 0.0 0.0 6.615955562324161 0.0 72-73 0.0 0.0 0.0 7.455173574651929 0.0 74-75 0.0 0.0 0.0 8.320441811813176 0.0 76-77 0.0 0.0 0.0 9.402528074126916 0.0 78-79 0.0 0.0 0.0 10.367187938345129 0.0 80-81 0.0 0.0 0.0 11.49736692342837 0.0 82-83 0.0 0.0 0.0 12.943354788031325 0.0 84-85 0.0 0.0 0.0 14.576102726055836 0.0 86-87 0.0 0.0 0.0 15.986421821271412 0.0 88-89 0.0 0.0 0.0 17.475693136366914 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TAAGGTG 50 2.8060986E-5 47.518044 5 GTAAGGT 60 1.4776488E-6 47.518044 4 CTACGTC 50 0.0016072944 38.014435 3 CGTCTAT 50 0.0016104655 37.9992 6 AAGGTGT 50 0.0016104655 37.9992 6 AGGTAAG 65 1.3184556E-4 36.55234 2 CCTACGT 55 0.0025660691 34.55858 2 TCCTACG 55 0.0025660691 34.55858 1 GGTAAGG 60 0.0039302614 31.678698 3 ACGTCTA 60 0.0039302614 31.678698 5 GGTTCAC 30 0.0039454843 31.665998 84-85 AGGGTGT 95 3.466717E-5 29.999367 6 TTGTAGG 65 0.005813576 29.241873 1 ACAGTAC 65 0.0058249845 29.230152 8 GTCTATC 65 0.0058249845 29.230152 7 TACATAA 70 0.008347933 27.153168 2 CTTCTAT 70 0.008347933 27.153168 1 TCCGCCC 35 0.008380066 27.142284 88-89 GTATATA 90 8.882991E-4 26.398912 1 GTTATAG 90 8.882991E-4 26.398912 1 >>END_MODULE