FastQCFastQC Report
Thu 26 May 2016
SRR937694_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937694_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences119921
Sequences flagged as poor quality0
Sequence length101
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16171.3483876885616364No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12601.0506917053727038No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7760.6470926693406492No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2860.2384903394734867No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT2750.22931763410912182No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2660.22181269335645967No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2640.22014492874475694No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT2350.19596234187506775No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT2220.18512187189900017No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC1930.16093928502931096No Hit
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT1540.12841787510110822No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1440.12007905204259471No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1290.10757081745482443No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGCTTG352.7723113E-454.3076555
GGTATCA7000.044.1433941
AGTACTA459.5714803E-442.2392855
GGACCAC459.591172E-442.221676
GTATCAA12250.041.5238951
TATCAAC15100.033.043152
ATCAACG15250.032.718143
CAACGCA15300.032.6112175
TCAACGC15400.032.3994524
CTAAGGA600.003929182431.6794663
TCTAGGA600.003929182431.6794662
GGACAGT753.0623787E-431.666256
GACAGTG600.003937223531.6662487
AACGCAG15800.031.566046
ACAGATC804.4702712E-429.687118
ACATAAT650.0058001329.2547931
ACGCAGA18050.027.6312167
CGCAGAG18100.027.5548868
ATGGGAG2450.027.1538285
AATACAG700.00834565927.1538285
Produced by FastQC (version 0.11.5)