FastQCFastQC Report
Thu 26 May 2016
SRR937694_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937694_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences119921
Sequences flagged as poor quality0
Sequence length101
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13461.1224055836759201No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11020.9189383010481901No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8100.6754446677395952No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT6480.5403557341916763No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA5550.46280467974750045No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2920.2434936333085948No Hit
GTCCCAGGGTGTGCTTGTCAAAGAGATATTCTGCCATGCCAGCTTCAGGG2600.21680939952135156No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2140.17845081345218936No Hit
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA2090.1742814019229326No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT1710.1425938743005812No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1360.11340799359578389No Hit
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT1320.11007246437237847No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1310.10923858206652715No Hit
CTACTGGAACTGCACAAACTGGCTACTGACAAGAATGATCCCCACTTATG1270.10590305284312172No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAAGGG255.2582527E-575.993662
GGACCGA250.005011996756.9952476
CATCGGT205.3707545E-447.51585486-87
AGGGTAG459.594503E-442.21875
TAAGGGT459.594503E-442.21873
GGTGTGC1051.0113581E-940.7108888
GACCGAG500.001610708437.996837
TAAGGTG500.001610708437.996835
GGTAGTT500.001610708437.996837
AGGGTGT1203.7362042E-935.622036
CCAGGGT958.619445E-734.9970784
TTGCGTA501.2943601E-633.24722760-61
GCGTAAG501.2943601E-633.24722762-63
TGCGTAA501.2943601E-633.24722760-61
CAGGGTG1201.487897E-731.6640265
AAGGGTA600.00393858331.6640264
CTTGCGT552.72334E-630.22475258-59
GCTATAC650.005802140629.252731
GACACGG650.00582586529.2283337
GGGTAGT650.00582586529.2283336