##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937694_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 119921 Sequences flagged as poor quality 0 Sequence length 101 %GC 41 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.358602746808316 33.0 31.0 34.0 31.0 34.0 2 32.55879287197405 34.0 31.0 34.0 31.0 34.0 3 32.60473978702646 34.0 31.0 34.0 31.0 34.0 4 36.20942954111457 37.0 35.0 37.0 35.0 37.0 5 36.14876460336388 37.0 35.0 37.0 35.0 37.0 6 36.217384778312386 37.0 36.0 37.0 35.0 37.0 7 36.16986182570192 37.0 36.0 37.0 35.0 37.0 8 36.16334920489322 37.0 36.0 37.0 35.0 37.0 9 37.83678421627572 39.0 38.0 39.0 35.0 39.0 10-11 37.78651362146746 39.0 37.5 39.0 35.0 39.0 12-13 37.70234154151483 39.0 37.0 39.0 35.0 39.0 14-15 38.85650136339757 40.0 38.0 41.0 35.0 41.0 16-17 38.8603247137699 40.0 38.0 41.0 35.5 41.0 18-19 38.91373070604815 40.0 38.0 41.0 35.0 41.0 20-21 38.844451764078016 40.0 38.0 41.0 35.0 41.0 22-23 38.695299405441915 40.0 38.0 41.0 34.5 41.0 24-25 38.71692197363264 40.0 38.0 41.0 35.0 41.0 26-27 38.54289073640146 40.0 38.0 41.0 34.0 41.0 28-29 38.395793897649284 40.0 38.0 41.0 34.0 41.0 30-31 38.3335612611636 40.0 37.5 41.0 34.0 41.0 32-33 38.154455850101314 40.0 37.0 41.0 34.0 41.0 34-35 37.96806647709742 40.0 37.0 41.0 34.0 41.0 36-37 37.764574178000515 40.0 36.5 41.0 33.0 41.0 38-39 37.53104543824685 40.0 36.0 41.0 33.0 41.0 40-41 37.29031195537062 40.0 36.0 41.0 33.0 41.0 42-43 36.97580907430725 40.0 36.0 41.0 32.5 41.0 44-45 36.49315799568049 40.0 35.0 41.0 31.0 41.0 46-47 35.87371686360187 39.0 35.0 41.0 29.0 41.0 48-49 35.3127392199865 39.0 35.0 41.0 26.5 41.0 50-51 34.926334837101095 39.0 35.0 41.0 24.0 41.0 52-53 34.63945430741905 39.0 35.0 40.0 23.0 41.0 54-55 34.37461745649219 38.5 35.0 40.0 21.5 41.0 56-57 34.0784808332152 38.0 34.0 40.0 19.5 41.0 58-59 33.802244811167355 38.0 34.0 40.0 17.0 41.0 60-61 33.354600111740226 38.0 33.5 40.0 10.0 41.0 62-63 33.00200548694558 37.0 33.0 40.0 7.5 41.0 64-65 32.61062282669424 36.5 33.0 39.5 2.0 41.0 66-67 32.14001300856397 36.0 32.0 39.0 2.0 40.5 68-69 31.673184846690738 35.5 32.0 39.0 2.0 40.0 70-71 31.17603255476522 35.0 31.0 38.5 2.0 40.0 72-73 30.642668923708108 35.0 31.0 37.5 2.0 40.0 74-75 30.13535994529732 35.0 30.0 37.0 2.0 39.0 76-77 28.93643315182495 33.5 28.5 35.5 2.0 38.5 78-79 29.46158721158096 34.0 30.0 36.0 2.0 38.5 80-81 29.258019863076527 34.0 30.0 36.0 2.0 37.5 82-83 28.92601379241334 34.0 29.0 35.0 2.0 37.0 84-85 28.54633883973616 34.0 29.0 35.0 2.0 37.0 86-87 28.113795748868007 34.0 29.0 35.0 2.0 36.0 88-89 27.7949691880488 34.0 28.0 35.0 2.0 36.0 90-91 27.313881638745507 34.0 26.5 35.0 2.0 35.0 92-93 26.87871598802545 33.0 25.5 35.0 2.0 35.0 94-95 26.442795673818594 33.0 25.0 35.0 2.0 35.0 96-97 25.92189858323396 33.0 24.0 35.0 2.0 35.0 98-99 25.131690863151576 32.5 19.0 34.5 2.0 35.0 100-101 22.735117285546316 29.5 2.0 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.24088614958013466 1101 2 0.199889778165236 1101 3 0.030248634138736463 1101 4 0.08905838491816809 1101 5 0.19590334431423173 1101 6 -0.12281628367937003 1101 7 -0.1643829449364631 1101 8 -0.12499998834073267 1101 9 0.11357595905572282 1101 10-11 0.020211471904787004 1101 12-13 0.006600804594611986 1101 14-15 0.10291515748411939 1101 16-17 0.031822500522935115 1101 18-19 0.019423893864768615 1101 20-21 0.22168739837050566 1101 22-23 0.0790149338681303 1101 24-25 0.0554571697189985 1101 26-27 0.36545507428256485 1101 28-29 -0.002549426426647017 1101 30-31 -0.13776486416676192 1101 32-33 0.2099133483695752 1101 34-35 0.33130871984774757 1101 36-37 0.3670206237125342 1101 38-39 0.4757644970265815 1101 40-41 0.22164346270464108 1101 42-43 0.5779889282536388 1101 44-45 0.5902245926143976 1101 46-47 -0.052404866618729784 1101 48-49 0.4013846054834147 1101 50-51 0.6378302057832457 1101 52-53 0.4054427057344938 1101 54-55 0.5082484381539274 1101 56-57 0.6834887446482512 1101 58-59 0.7772175872054632 1101 60-61 0.9878647247923169 1101 62-63 1.2877558035634422 1101 64-65 1.3077141920326874 1101 66-67 1.43479648638181 1101 68-69 1.2990230761179546 1101 70-71 1.2731143494675798 1101 72-73 1.2346242055893946 1101 74-75 1.2722897961336663 1101 76-77 0.7769103489538729 1101 78-79 1.146834844235034 1101 80-81 1.5584202136527203 1101 82-83 1.4801548208299344 1101 84-85 1.4312128322728022 1101 86-87 1.1259032284848374 1101 88-89 0.8962136905536511 1101 90-91 0.4191733883716111 1101 92-93 0.44864870665336554 1101 94-95 0.6154539527461615 1101 96-97 0.9196980388836842 1101 98-99 1.2951593729760695 1101 100-101 0.48534078157268823 1102 1 0.28983274627715616 1102 2 0.2651197519266191 1102 3 0.17639387260145867 1102 4 0.14753577340404433 1102 5 0.15158709180612817 1102 6 0.16823943479570858 1102 7 0.1252257909795773 1102 8 0.04622628114529448 1102 9 -0.06339965446488094 1102 10-11 0.15962341819279402 1102 12-13 0.2105867592172359 1102 14-15 -0.15537122930396663 1102 16-17 -0.05292244468466123 1102 18-19 0.09633584015277563 1102 20-21 0.015058288940039688 1102 22-23 0.11147557594252788 1102 24-25 0.37435283262994545 1102 26-27 -0.24157340233419689 1102 28-29 0.06393459888590058 1102 30-31 -0.2687071392092051 1102 32-33 -0.3519789189556235 1102 34-35 0.018008056165079722 1102 36-37 0.4264819603385561 1102 38-39 0.24662033897750035 1102 40-41 -0.15727303675515714 1102 42-43 -0.021910747622264637 1102 44-45 0.26034150880516904 1102 46-47 -0.09137693020879567 1102 48-49 0.40048747629681003 1102 50-51 -0.2649711524510465 1102 52-53 -0.5992069315552087 1102 54-55 -0.5367913056336917 1102 56-57 -0.566723479460407 1102 58-59 -0.42056563551409454 1102 60-61 -0.21780937614919083 1102 62-63 -0.40866725555061834 1102 64-65 0.02500273964805544 1102 66-67 -0.012938503585768757 1102 68-69 0.225226965607078 1102 70-71 -0.2347958218551618 1102 72-73 0.23456632628703034 1102 74-75 0.2758011404769256 1102 76-77 0.5354186064010129 1102 78-79 0.8283925691925909 1102 80-81 1.0241749597555128 1102 82-83 0.7943526136992034 1102 84-85 0.657482318304595 1102 86-87 0.7426457813479317 1102 88-89 0.613733755378469 1102 90-91 0.3935521505262649 1102 92-93 0.5915044714318789 1102 94-95 0.6171999783673989 1102 96-97 1.018063913247012 1102 98-99 0.8540322700280818 1102 100-101 0.4145447482342135 1103 1 -0.09208657530840725 1103 2 0.014176429096259824 1103 3 -0.2863983629763993 1103 4 -0.1649083079745708 1103 5 -0.07379898980588706 1103 6 -0.43116928656423426 1103 7 -0.16041625204372423 1103 8 -0.006949896549492962 1103 9 0.1657986183790925 1103 10-11 0.14458860277287044 1103 12-13 -0.19943774772639244 1103 14-15 -0.14801914621376255 1103 16-17 -0.2154073709693307 1103 18-19 -0.28861767445413733 1103 20-21 -0.4205234592219469 1103 22-23 0.04608482634125011 1103 24-25 0.08978053714622547 1103 26-27 -0.02814904321022027 1103 28-29 -0.35351979096899555 1103 30-31 -0.29908796933329285 1103 32-33 -0.18636254987328016 1103 34-35 -0.22392993737753386 1103 36-37 0.008969220617856877 1103 38-39 -0.6111421381688089 1103 40-41 -0.13424428972126634 1103 42-43 0.5880203995360986 1103 44-45 1.0569338766427236 1103 46-47 0.3534435467041135 1103 48-49 -0.23326986606130617 1103 50-51 -0.40275463554903723 1103 52-53 0.35249882952154366 1103 54-55 0.38657587062180454 1103 56-57 0.34769671737313956 1103 58-59 0.44563812221726096 1103 60-61 0.21545782733623753 1103 62-63 0.565522653812593 1103 64-65 0.9375168408656229 1103 66-67 1.238199318797239 1103 68-69 1.2354576420765184 1103 70-71 0.8636598516967915 1103 72-73 0.5360600828513924 1103 74-75 0.11119616906836072 1103 76-77 3.8923696856230094E-4 1103 78-79 0.47687352768638647 1103 80-81 0.6559484066765862 1103 82-83 1.2269585187056222 1103 84-85 0.546345273919794 1103 86-87 1.1338038316844177 1103 88-89 1.4544191718020123 1103 90-91 1.2653574953739728 1103 92-93 1.3266155306764418 1103 94-95 1.3371789724157104 1103 96-97 1.131457152442561 1103 98-99 0.6256897952157736 1103 100-101 0.5432840021339302 1104 1 -0.19387964803365776 1104 2 0.200165960224453 1104 3 -0.24819811796024993 1104 4 -0.255230170097299 1104 5 -0.19920270959650566 1104 6 0.03747978348810932 1104 7 0.17648095254475749 1104 8 0.08771543366545131 1104 9 -0.01529058977326514 1104 10-11 -0.305572885143377 1104 12-13 -0.15750657499000909 1104 14-15 0.1904372520488522 1104 16-17 0.16369943379734764 1104 18-19 0.20648200257002713 1104 20-21 0.2958146630633891 1104 22-23 -0.20277362114733677 1104 24-25 -0.3101526402677379 1104 26-27 0.48766563548451103 1104 28-29 -0.018126094566284223 1104 30-31 0.369360632960948 1104 32-33 0.636780339089853 1104 34-35 -0.14830904418229807 1104 36-37 0.010082930385088673 1104 38-39 0.7132258315382884 1104 40-41 0.6533712576670823 1104 42-43 0.8372686454432099 1104 44-45 1.0171744379524412 1104 46-47 1.3570074179592666 1104 48-49 0.7823777561683443 1104 50-51 0.6092177444487348 1104 52-53 1.123018931982834 1104 54-55 1.6691797240575994 1104 56-57 1.4037163186650474 1104 58-59 1.8388890410278194 1104 60-61 1.339803522847987 1104 62-63 1.5607893873018455 1104 64-65 0.9892486595536774 1104 66-67 1.6299467294220271 1104 68-69 1.5627769426667868 1104 70-71 1.7456066163699155 1104 72-73 0.616915411952629 1104 74-75 0.7922853772207183 1104 76-77 0.9191441094487942 1104 78-79 1.7061863687600045 1104 80-81 0.8021784991144969 1104 82-83 0.29795751636683576 1104 84-85 0.5918403179113305 1104 86-87 0.8527591719227523 1104 88-89 1.2472134696080026 1104 90-91 0.4275693229716957 1104 92-93 -0.31866790845931803 1104 94-95 -0.09165497345799523 1104 96-97 -0.08900169598154051 1104 98-99 -0.21282228853520024 1104 100-101 0.06722876212947426 1105 1 -0.058185670261622136 1105 2 0.03319775138495373 1105 3 0.024999090620781317 1105 4 0.03450878053593698 1105 5 -0.6590114457040315 1105 6 -0.4595478722368824 1105 7 0.03639307454010776 1105 8 0.005782117563100542 1105 9 0.19018886228153065 1105 10-11 -0.0894963795863859 1105 12-13 -0.12235537312906786 1105 14-15 -0.19725982729981695 1105 16-17 -0.007016513475846864 1105 18-19 -0.2848594293245199 1105 20-21 -0.4090186271924878 1105 22-23 -0.5451101422122164 1105 24-25 -0.7134868351501424 1105 26-27 -0.527842247689442 1105 28-29 -0.7954587386603649 1105 30-31 -1.5025700984942105 1105 32-33 -1.387773809268893 1105 34-35 -1.9969779508765342 1105 36-37 -2.9794492484724984 1105 38-39 -2.8441533362053164 1105 40-41 -1.9758611021158146 1105 42-43 -1.5709763791109381 1105 44-45 -1.4423758334355128 1105 46-47 -2.0755332902340697 1105 48-49 -2.5502663379128165 1105 50-51 -2.2098569518552225 1105 52-53 -2.4503850483738425 1105 54-55 -2.106214434639739 1105 56-57 -2.4480541646639793 1105 58-59 -2.509262936227284 1105 60-61 -2.38816235980903 1105 62-63 -2.0285101733081348 1105 64-65 -2.049521048381191 1105 66-67 -2.1762047309036383 1105 68-69 -2.5428058952758334 1105 70-71 -3.307378651141068 1105 72-73 -2.4646302070703747 1105 74-75 -2.205646905022757 1105 76-77 -2.3619362730789923 1105 78-79 -1.1884892580169435 1105 80-81 -1.3715097924337059 1105 82-83 -0.9946245461816261 1105 84-85 -1.8621529620059611 1105 86-87 -2.63832379525223 1105 88-89 -3.097966163372096 1105 90-91 -3.5388302634847513 1105 92-93 -2.9139121576193077 1105 94-95 -3.218044221325659 1105 96-97 -2.5276163250846686 1105 98-99 -2.7987618077908216 1105 100-101 -2.570920630478433 1106 1 -0.07091054959466447 1106 2 0.3798410159746908 1106 3 0.10573625604421011 1106 4 0.15254300607345073 1106 5 0.40417416201865564 1106 6 0.1999519083686465 1106 7 0.28957429437336657 1106 8 -0.005626394942389368 1106 9 -0.2749274167882376 1106 10-11 -0.6270128341717296 1106 12-13 -0.39484330643314536 1106 14-15 0.3457414890670094 1106 16-17 0.16784088011782927 1106 18-19 -0.3996027377931455 1106 20-21 0.03920427759030787 1106 22-23 -0.05081439846495783 1106 24-25 0.49647367384503127 1106 26-27 0.5520173398489518 1106 28-29 0.6784333192597742 1106 30-31 0.6584654434101296 1106 32-33 0.8006860415428747 1106 34-35 0.93961704692952 1106 36-37 0.5352501811018797 1106 38-39 0.26993178884954006 1106 40-41 0.05397654597545909 1106 42-43 -0.6907657604185289 1106 44-45 0.4233547501537842 1106 46-47 0.8073100621134515 1106 48-49 0.7603962333903951 1106 50-51 0.2227889454681673 1106 52-53 -0.2917628456533521 1106 54-55 -0.4155606391153839 1106 56-57 -0.9326965516758321 1106 58-59 -0.537345428548484 1106 60-61 -0.3374822369481265 1106 62-63 0.4945262461740967 1106 64-65 0.278034897208979 1106 66-67 -0.7558449239707734 1106 68-69 -1.1382424902736403 1106 70-71 -0.7360754479817864 1106 72-73 -0.35361660461315836 1106 74-75 0.005410576328714001 1106 76-77 0.13784433244974892 1106 78-79 0.03463491925379003 1106 80-81 0.10896409962421316 1106 82-83 -1.3825139713681125 1106 84-85 -0.1486821414706334 1106 86-87 -0.39238259297052736 1106 88-89 -0.8618977122970612 1106 90-91 -0.4986810752442956 1106 92-93 -0.8028985551620913 1106 94-95 -0.4881188154458904 1106 96-97 0.20472241997896035 1106 98-99 -0.2263616322752462 1106 100-101 -0.8567916265293363 1107 1 0.3739506657091667 1107 2 0.3813413910684602 1107 3 0.4133131502677685 1107 4 0.18180032040203287 1107 5 0.12735495721745593 1107 6 -0.03410660626001061 1107 7 0.10577834538612052 1107 8 0.30645162456249153 1107 9 0.3192211203460502 1107 10-11 0.028275988033819033 1107 12-13 0.15579435298170807 1107 14-15 -0.13700419735459946 1107 16-17 0.5781934682648711 1107 18-19 0.30369808741315296 1107 20-21 -0.2702480855004623 1107 22-23 0.36328912741651465 1107 24-25 0.5111023310093188 1107 26-27 -0.35833524829808283 1107 28-29 -0.09125910384599933 1107 30-31 0.20497707131710996 1107 32-33 0.41959076772441506 1107 34-35 0.6901796875896835 1107 36-37 1.1654077204867264 1107 38-39 0.4233451421878698 1107 40-41 -1.3751307308437504 1107 42-43 -0.5752368781979769 1107 44-45 -1.1617915364178586 1107 46-47 -1.4395016408122814 1107 48-49 -0.6086960396778807 1107 50-51 -0.20894398776513867 1107 52-53 -0.32439600394292256 1107 54-55 -1.1147354328138164 1107 56-57 -1.2882854489001332 1107 58-59 -1.3497985418267966 1107 60-61 -0.9657643074657543 1107 62-63 -1.6033732286946218 1107 64-65 -1.4080116144189283 1107 66-67 -1.674074481360119 1107 68-69 -3.446944665817526 1107 70-71 -2.912369521500164 1107 72-73 -2.6000532137654453 1107 74-75 -2.0422263328985935 1107 76-77 -1.3884122316912872 1107 78-79 -2.32695547834561 1107 80-81 -2.5665797863472797 1107 82-83 -3.3363774372345816 1107 84-85 -2.7945936193401018 1107 86-87 -1.7652258037732302 1107 88-89 -1.158221793317317 1107 90-91 -1.500181450338065 1107 92-93 -2.1138512933466345 1107 94-95 -1.2454331440280306 1107 96-97 -0.27989873530986387 1107 98-99 -0.2229857883142543 1107 100-101 -0.1981269603627922 1108 1 0.553674168013309 1108 2 -0.08593971492231844 1108 3 0.4206864221571678 1108 4 0.08410446786747627 1108 5 -0.18785241605443304 1108 6 -0.25253978598351523 1108 7 -0.4094290278857713 1108 8 -0.51658985009189 1108 9 0.1330460051386737 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-0.1153240617640563 2316 48-49 0.5158648035032201 2316 50-51 1.2386765807433235 2316 52-53 1.095757301710222 2316 54-55 0.3823900861897016 2316 56-57 -0.4497354648694696 2316 58-59 -0.5548448529095182 2316 60-61 -0.5085621356356711 2316 62-63 -0.12509152955696123 2316 64-65 -0.7050413173892274 2316 66-67 -0.527316257150602 2316 68-69 0.08786849291769983 2316 70-71 -0.042200246508148354 2316 72-73 0.14315981721507143 2316 74-75 0.027399984571861324 2316 76-77 0.18999722215732717 2316 78-79 0.6139579678377167 2316 80-81 -0.13061683521345913 2316 82-83 0.041616813803425856 2316 84-85 0.1857640938505689 2316 86-87 -0.45973235120216316 2316 88-89 -0.6219713940838467 2316 90-91 -0.28028365410683165 2316 92-93 -0.647706291749703 2316 94-95 0.3011973254705289 2316 96-97 -0.16380164172953826 2316 98-99 0.23645209095754538 2316 100-101 0.9895127713523131 >>END_MODULE >>Per sequence quality scores pass #Quality Count 4 2.0 5 5.0 6 4.0 7 17.0 8 34.0 9 61.0 10 119.0 11 198.0 12 375.0 13 553.0 14 715.0 15 1127.0 16 1614.0 17 2349.0 18 2099.0 19 1196.0 20 923.0 21 903.0 22 954.0 23 963.0 24 1031.0 25 1145.0 26 1263.0 27 1350.0 28 1658.0 29 1934.0 30 2230.0 31 2853.0 32 3707.0 33 4986.0 34 7076.0 35 10872.0 36 16566.0 37 25596.0 38 21396.0 39 2047.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.86434189345855 20.460895602058436 15.229738859187469 19.445023645295546 2 15.582758649444218 22.265491448536952 40.26067160880913 21.891078293209695 3 19.963976284387222 27.188732582283336 30.590138507851 22.25715262547844 4 12.19302707615847 18.066060156269543 37.84741621567532 31.893496551896668 5 12.090249395279006 39.64967887229961 33.44399032446409 14.816081407957293 6 29.531941861725635 38.10008255434828 16.930312455700005 15.437663128226081 7 26.598343909740578 36.482350880996655 19.95730522594041 16.961999983322354 8 26.72759566714754 34.501880404599696 19.147605506958747 19.62291842129402 9 27.48392691855471 17.55072089125341 20.330884498961815 34.63446769123006 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27.88377348421044 32.86622026167227 19.277274205518633 36-37 20.57354424996456 27.35550904345361 33.06551813277074 19.00542857381109 38-39 19.851402173097288 27.965493950183873 32.909582141576536 19.273521735142303 40-41 19.839975983889193 28.25979094309099 32.48679322378762 19.41343984923219 42-43 19.80468033292746 28.508164729037745 31.79115306990476 19.896001868130035 44-45 20.315457676303566 28.34282569358161 31.57828904028485 19.76342758982997 46-47 20.548110839636095 29.086231769248087 30.99790695541231 19.367750435703503 48-49 20.987983755972685 29.046205418567222 30.187373354124798 19.778437471335295 50-51 20.511836959331557 29.317217167968913 30.30786934732032 19.86307652537921 52-53 20.402598377265033 30.040193127142032 29.27427222921757 20.282936266375366 54-55 19.98440640088058 29.919697133946517 29.516515039067386 20.57938142610552 56-57 20.51558942970789 29.712477380942452 29.700803028660534 20.071130160689123 58-59 20.70071130160689 28.89819130927861 29.8642439606074 20.5368534285071 60-61 20.029019104243627 29.208395527055313 30.64350697542549 20.119078393275572 62-63 19.89267934723693 28.813552255234697 30.774843438597077 20.518924958931297 64-65 19.62375230359987 28.913201190783933 30.72355967678722 20.73948682882898 66-67 19.53869630840303 28.776444492624307 30.380417107929386 21.30444209104327 68-69 19.68170712385654 28.799376256035224 29.88550795940661 21.63340866070163 70-71 19.923949933706357 29.127925884540655 29.808790787268286 21.1393333944847 72-73 19.757173472536085 28.39285863193269 30.263673585110197 21.586294310421028 74-75 19.258928794789902 29.1108312972707 29.107912709200225 22.52232719873917 76-77 19.47240266508785 28.09015935490865 29.94304583851035 22.49439214149315 78-79 19.59206477597752 28.605915561077712 29.358494342108553 22.443525320836216 80-81 19.253925500954796 28.326981929770433 29.388931046272127 23.030161523002644 82-83 18.949457550513262 28.197366555758467 29.373994112692735 23.479181781035532 84-85 18.47849200095063 28.269561918104074 29.655480088892222 23.596465992053066 86-87 18.66311988725911 28.3215616947824 28.977410128334487 24.037908289624003 88-89 18.068978744340026 27.881688778445813 29.046205418567222 25.00312705864694 90-91 18.148382899938284 27.78718329357997 29.356329124480844 24.7081046820009 92-93 18.19243149485482 27.78607048149569 29.27875715071966 24.742740872929836 94-95 17.749601821198958 28.42663086531967 28.418292042261157 25.40547527122022 96-97 17.678721825201592 29.401856222012825 28.000933948182556 24.91848800460303 98-99 18.132466070423416 29.67581879208623 26.826776742343693 25.364938395146662 100-101 17.433461503173664 28.719358094300752 26.894814542967477 26.952365859558107 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 234.0 1 150.5 2 84.5 3 101.5 4 105.0 5 115.5 6 158.0 7 267.5 8 381.0 9 449.0 10 459.0 11 427.0 12 417.0 13 450.0 14 491.0 15 502.0 16 500.0 17 506.0 18 492.0 19 508.5 20 577.0 21 634.0 22 690.0 23 776.5 24 860.5 25 979.5 26 1145.5 27 1311.0 28 1473.5 29 1677.5 30 1910.5 31 2091.0 32 2219.5 33 2426.0 34 2676.5 35 2780.0 36 3015.5 37 3311.5 38 3461.0 39 3518.0 40 3552.5 41 3718.0 42 3829.0 43 3967.0 44 4078.0 45 4108.5 46 4072.0 47 4332.5 48 4918.0 49 4686.5 50 4064.0 51 3687.0 52 3525.5 53 3434.0 54 3165.0 55 2814.0 56 2418.5 57 2155.0 58 1925.0 59 1684.0 60 1370.0 61 1074.5 62 856.5 63 626.5 64 419.5 65 285.0 66 228.5 67 200.5 68 150.0 69 83.5 70 58.5 71 46.0 72 38.0 73 34.0 74 24.5 75 17.0 76 13.0 77 8.5 78 7.5 79 6.5 80 7.0 81 5.0 82 2.0 83 2.5 84 3.0 85 2.0 86 1.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.02001317534043245 2 0.0 3 0.0 4 0.0 5 0.02585035148139192 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.00208470576462838 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 4.16941152925676E-4 24-25 0.0 26-27 4.16941152925676E-4 28-29 8.33882305851352E-4 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0012508234587770282 42-43 0.012508234587770282 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 0.001667764611702704 84-85 4.16941152925676E-4 86-87 0.0 88-89 0.0 90-91 0.012508234587770282 92-93 0.0025016469175540563 94-95 0.0 96-97 0.0 98-99 0.0029185880704797325 100-101 0.02334870456383786 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 119921.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 72.17334745374038 #Duplication Level Percentage of deduplicated Percentage of total 1 88.05675266605816 63.55350605815495 2 7.178426592413721 10.361821532508902 3 2.011530773763446 4.355367283461612 4 0.9763029889891509 2.81852219377757 5 0.452912155838754 1.6344093194686504 6 0.2888470381624707 1.2508234587770282 7 0.2021929267137295 1.0215058246679063 8 0.14557890723388522 0.840553364298163 9 0.10629571004378921 0.6904545492449196 >10 0.5326339383715959 6.560152100132586 >50 0.026573927510947302 1.3600620408435553 >100 0.016175434137098356 1.8328733082612718 >500 0.003466164457949648 1.6786050816787719 >1k 0.002310776305299765 2.04134388472411 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1346 1.1224055836759201 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1102 0.9189383010481901 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 810 0.6754446677395952 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 648 0.5403557341916763 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 555 0.46280467974750045 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 292 0.2434936333085948 No Hit GTCCCAGGGTGTGCTTGTCAAAGAGATATTCTGCCATGCCAGCTTCAGGG 260 0.21680939952135156 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 214 0.17845081345218936 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 209 0.1742814019229326 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 171 0.1425938743005812 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 136 0.11340799359578389 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 132 0.11007246437237847 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 131 0.10923858206652715 No Hit CTACTGGAACTGCACAAACTGGCTACTGACAAGAATGATCCCCACTTATG 127 0.10590305284312172 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 4.169411529256761E-4 0.0 12-13 0.0 0.0 0.0 8.338823058513521E-4 0.0 14-15 0.0 0.0 0.0 0.0012508234587770282 0.0 16-17 0.0 0.0 0.0 0.0025016469175540563 0.0 18-19 0.0 0.0 0.0 0.0033355292234054086 0.0 20-21 0.0 0.0 0.0 0.0033355292234054086 0.0 22-23 0.0 0.0 0.0 0.0045863526821824365 0.0 24-25 0.0 0.0 0.0 0.005837176140959465 0.0 26-27 0.0 0.0 0.0 0.008755764211439197 0.0 28-29 0.0 0.0 0.0 0.016260704964101366 0.0 30-31 0.0 0.0 0.0 0.02710117494016894 0.0 32-33 0.0 0.0 0.0 0.03794164491623652 0.0 34-35 0.0 0.0 0.0 0.049199056045229776 0.0 36-37 0.0 0.0 0.0 0.06671058446810817 0.0 38-39 0.0 0.0 0.0 0.0958964651729055 0.0 40-41 0.0 0.0 0.0 0.16469175540564204 0.0 42-43 0.0 0.0 0.0 0.27726586669557457 0.0 44-45 0.0 0.0 0.0 0.3802503314682166 0.0 46-47 0.0 0.0 0.0 0.6600178450813452 0.0 48-49 0.0 0.0 0.0 0.9381175940827711 0.0 50-51 0.0 0.0 0.0 1.1357477005695416 0.0 52-53 0.0 0.0 0.0 1.3696516873608457 0.0 54-55 0.0 0.0 0.0 1.6035556741521502 0.0 56-57 0.0 0.0 0.0 2.141409761426272 0.0 58-59 0.0 0.0 0.0 2.746808315474354 0.0 60-61 0.0 0.0 0.0 3.386396044062341 0.0 62-63 0.0 0.0 0.0 4.097280709800619 0.0 64-65 0.0 0.0 0.0 4.876543724618708 0.0 66-67 0.0 0.0 0.0 5.736276381951451 0.0 68-69 0.0 0.0 0.0 6.665221270669858 0.0 70-71 0.0 0.0 0.0 7.591247571317784 0.0 72-73 0.0 0.0 0.0 8.505182578530865 0.0 74-75 0.0 0.0 0.0 9.486245111364983 0.0 76-77 0.0 0.0 0.0 10.711218218660619 0.0 78-79 0.0 0.0 0.0 11.74481533676337 0.0 80-81 0.0 0.0 0.0 12.9973065601521 0.0 82-83 0.0 0.0 0.0 14.50454882797842 0.0 84-85 0.0 0.0 0.0 16.270294610618656 0.0 86-87 0.0 0.0 0.0 17.81464464105536 0.0 88-89 0.0 0.0 0.0 19.423620550195544 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CTAAGGG 25 5.2582527E-5 75.99366 2 GGACCGA 25 0.0050119967 56.995247 6 CATCGGT 20 5.3707545E-4 47.515854 86-87 AGGGTAG 45 9.594503E-4 42.2187 5 TAAGGGT 45 9.594503E-4 42.2187 3 GGTGTGC 105 1.0113581E-9 40.710888 8 GACCGAG 50 0.0016107084 37.99683 7 TAAGGTG 50 0.0016107084 37.99683 5 GGTAGTT 50 0.0016107084 37.99683 7 AGGGTGT 120 3.7362042E-9 35.62203 6 CCAGGGT 95 8.619445E-7 34.997078 4 TTGCGTA 50 1.2943601E-6 33.247227 60-61 GCGTAAG 50 1.2943601E-6 33.247227 62-63 TGCGTAA 50 1.2943601E-6 33.247227 60-61 CAGGGTG 120 1.487897E-7 31.664026 5 AAGGGTA 60 0.003938583 31.664026 4 CTTGCGT 55 2.72334E-6 30.224752 58-59 GCTATAC 65 0.0058021406 29.25273 1 GACACGG 65 0.005825865 29.228333 7 GGGTAGT 65 0.005825865 29.228333 6 >>END_MODULE