FastQCFastQC Report
Thu 26 May 2016
SRR937693_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937693_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences94654
Sequences flagged as poor quality0
Sequence length101
%GC40

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13961.4748452257696454No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10531.1124727956557567No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7490.7913030616772667No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT2480.2620068882456103No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2330.24615969742430324No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2290.2419337798719547No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2130.22503010966256048No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT1960.20706996006507913No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT1910.2017875631246434No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC1790.18910981046759778No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1390.14685063494411224No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN1310.13839879983941514No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1310.13839879983941514No Hit
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT1160.12255160901810805No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1040.10987385636106241No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT960.10142202125636528No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGTAGC156.595147E-495.013782
CCATACT301.3564386E-679.178154
CCCATAC200.002066973671.260333
GTTAGAG301.2941414E-463.3425182
GTAGTAG250.00498318957.0687521
GGTTAGA352.7583272E-454.3511961
ATTGGCG205.3728267E-447.50688616-17
TTAGAGC459.5733133E-442.2283443
GGTATCA4900.039.792841
ATTACGA250.001611246138.00551234-35
GCGGCGC250.001611246138.00551220-21
GTATCAA7700.036.4400021
ATGGGAT1651.8189894E-1234.550475
TAGAGCC550.002565866634.5504654
GTACAGT702.0373936E-433.9334956
ATGTACA851.61484E-533.5342754
ATGGGGA600.00392996531.6712595
ATGGGAG1201.4792022E-731.6712597
CGGCGCT300.00393986331.67125922-23
CTCATAA300.00393986331.67125950-51
Produced by FastQC (version 0.11.5)