##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937693_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 94654 Sequences flagged as poor quality 0 Sequence length 101 %GC 40 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.98096224142667 33.0 31.0 34.0 31.0 34.0 2 32.09898155386988 33.0 31.0 34.0 31.0 34.0 3 32.080672766074336 34.0 31.0 34.0 30.0 34.0 4 35.68351046971073 37.0 35.0 37.0 35.0 37.0 5 35.6801086060811 37.0 35.0 37.0 35.0 37.0 6 35.75181186215057 37.0 35.0 37.0 35.0 37.0 7 35.727840344834874 37.0 35.0 37.0 35.0 37.0 8 35.70682696980582 37.0 35.0 37.0 35.0 37.0 9 37.38901684028145 39.0 37.0 39.0 35.0 39.0 10-11 37.295771969488875 39.0 37.0 39.0 35.0 39.0 12-13 37.16438291038942 39.0 37.0 39.0 34.0 39.0 14-15 38.30079552897923 40.0 38.0 41.0 34.0 41.0 16-17 38.28615800705728 40.0 38.0 41.0 34.0 41.0 18-19 38.317635810425344 40.0 38.0 41.0 34.0 41.0 20-21 38.308798360343985 40.0 38.0 41.0 34.0 41.0 22-23 38.11500834618717 40.0 38.0 41.0 34.0 41.0 24-25 37.969008177150464 40.0 38.0 41.0 33.5 41.0 26-27 37.865383396369936 40.0 38.0 41.0 33.5 41.0 28-29 37.83713313753249 40.0 38.0 41.0 33.0 41.0 30-31 37.53190567752023 40.0 37.0 41.0 33.0 41.0 32-33 37.19308745536375 39.5 35.5 41.0 33.0 41.0 34-35 37.11844718659539 40.0 36.0 41.0 33.0 41.0 36-37 36.96433853825512 40.0 35.0 41.0 32.5 41.0 38-39 36.77053267690748 40.0 35.0 41.0 31.5 41.0 40-41 36.4239176368669 39.5 35.0 41.0 30.5 41.0 42-43 36.06031969066284 39.0 35.0 41.0 30.0 41.0 44-45 35.601680858706445 39.0 35.0 41.0 28.0 41.0 46-47 34.977032138102985 39.0 35.0 41.0 24.0 41.0 48-49 34.38141547108416 39.0 35.0 41.0 17.5 41.0 50-51 33.35422697403173 38.0 34.0 39.5 10.0 40.5 52-53 33.39123016459949 38.0 33.5 39.5 7.5 40.5 54-55 33.65236545734993 38.0 34.0 40.0 2.0 41.0 56-57 33.38218141864052 38.0 34.0 40.0 2.0 41.0 58-59 33.122947788788636 38.0 34.0 40.0 2.0 41.0 60-61 32.76394024552581 38.0 33.0 40.0 2.0 41.0 62-63 32.40231263338052 37.5 33.0 40.0 2.0 41.0 64-65 32.01881061550489 37.0 32.5 40.0 2.0 41.0 66-67 31.579853994548564 36.0 32.0 39.5 2.0 41.0 68-69 31.090767426627508 36.0 31.0 39.0 2.0 41.0 70-71 30.62849958797304 35.0 31.0 39.0 2.0 40.0 72-73 30.173167536501364 35.0 30.0 38.5 2.0 40.0 74-75 29.717439305259155 35.0 29.5 37.5 2.0 40.0 76-77 29.23773427430431 35.0 29.0 37.0 2.0 39.0 78-79 28.73038117776322 34.5 29.0 36.5 2.0 39.0 80-81 28.22683668941619 34.0 27.5 36.0 2.0 38.5 82-83 27.725067086441143 34.0 26.0 35.5 2.0 37.0 84-85 27.274542016185265 34.0 26.0 35.0 2.0 37.0 86-87 26.832347285904454 34.0 25.0 35.0 2.0 36.0 88-89 26.357972193462505 33.5 24.5 35.0 2.0 36.0 90-91 25.877791746783018 33.0 21.5 35.0 2.0 35.5 92-93 25.354448834703234 33.0 19.0 35.0 2.0 35.0 94-95 24.74978870412238 32.0 11.0 35.0 2.0 35.0 96-97 24.075443193103304 32.0 2.0 34.5 2.0 35.0 98-99 23.184609208274345 31.5 2.0 34.0 2.0 35.0 100-101 21.093979123967294 28.0 2.0 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.3202742266329537 1101 2 0.36048500055019517 1101 3 0.21231803164763008 1101 4 0.20705658714432218 1101 5 0.23886746244434676 1101 6 0.2293795275290904 1101 7 0.16728896053080433 1101 8 0.3184335263766158 1101 9 0.3223663348201171 1101 10-11 0.5991908796476579 1101 12-13 0.3274305491444025 1101 14-15 0.46934033776549455 1101 16-17 0.5357810080990859 1101 18-19 0.683557983211081 1101 20-21 0.6576234421551703 1101 22-23 0.9155173434068402 1101 24-25 0.6843465276375866 1101 26-27 0.7461592618016368 1101 28-29 0.4814698606812584 1101 30-31 0.8688613045285436 1101 32-33 0.9392828195446583 1101 34-35 0.8999832731561952 1101 36-37 1.4937698810778173 1101 38-39 1.4089978147664723 1101 40-41 1.873473564346206 1101 42-43 2.0138108094849727 1101 44-45 2.11684160183885 1101 46-47 2.3731266294612325 1101 48-49 2.4955735126694307 1101 50-51 2.7821824836483344 1101 52-53 2.9782577931486713 1101 54-55 3.362263152884509 1101 56-57 3.156646961098801 1101 58-59 3.5160635693844284 1101 60-61 3.1196404195913736 1101 62-63 3.671296762475677 1101 64-65 3.7318670098206077 1101 66-67 3.8714871028433784 1101 68-69 3.718687770543987 1101 70-71 3.9216898444919437 1101 72-73 3.5449080422296504 1101 74-75 3.4120429538248267 1101 76-77 3.508099052485864 1101 78-79 3.185069339279842 1101 80-81 3.3970340089196966 1101 82-83 3.4124905700869554 1101 84-85 3.130672469843251 1101 86-87 2.6184308322880483 1101 88-89 2.616049554089262 1101 90-91 2.6714608922064116 1101 92-93 3.028543147129433 1101 94-95 2.520794708582155 1101 96-97 2.3961856042747094 1101 98-99 2.398980333566339 1101 100-101 3.1670649610998503 1102 1 0.6482742266329495 1102 2 0.6884850005501981 1102 3 0.7736513649809673 1102 4 0.35372325381099046 1102 5 0.7055341291110153 1102 6 0.4733795275290902 1102 7 0.43395562719747005 1102 8 0.2877668597099472 1102 9 0.8410330014867853 1102 10-11 0.4265242129809934 1102 12-13 0.6754305491444086 1102 14-15 0.8693403377654931 1102 16-17 0.9784476747657536 1102 18-19 0.44755798321107676 1102 20-21 0.5342901088218426 1102 22-23 0.5541840100735058 1102 24-25 0.8223465276375848 1102 26-27 0.6621592618016336 1102 28-29 0.4341365273479241 1102 30-31 0.9241946378618735 1102 32-33 0.8206161528779958 1102 34-35 1.5793166064895274 1102 36-37 1.7031032144111506 1102 38-39 1.7043311480998042 1102 40-41 1.9481402310128786 1102 42-43 1.9398108094849746 1102 44-45 2.178174935172187 1102 46-47 2.3731266294612325 1102 48-49 2.825573512669429 1102 50-51 2.574182483648336 1102 52-53 2.5609244598153396 1102 54-55 2.658929819551176 1102 56-57 2.8393136277654634 1102 58-59 2.9733969027177594 1102 60-61 2.7503070862580365 1102 62-63 3.287296762475677 1102 64-65 3.1565336764872747 1102 66-67 3.551487102843371 1102 68-69 3.880687770543986 1102 70-71 3.4270231778252764 1102 72-73 3.1929080422296465 1102 74-75 3.3180429538248255 1102 76-77 3.3540990524858607 1102 78-79 3.646402672613174 1102 80-81 3.3910340089196964 1102 82-83 3.204490570086957 1102 84-85 3.137339136509919 1102 86-87 3.2744308322880507 1102 88-89 3.352049554089259 1102 90-91 3.427460892206412 1102 92-93 3.325876480462764 1102 94-95 3.796794708582155 1102 96-97 3.1308522709413786 1102 98-99 3.5843136668996713 1102 100-101 4.5183982944331795 1103 1 0.16669397971936917 1103 2 4.850005501957355E-4 1103 3 -0.26595357329063773 1103 4 0.04804424146531261 1103 5 0.24676869701225002 1103 6 -0.04395380580424302 1103 7 -0.019377706135863093 1103 8 0.27472982267291 1103 9 0.4979218903756717 1103 10-11 0.3274624845859293 1103 12-13 0.36743054914440876 1103 14-15 0.11353786862969173 1103 16-17 -0.04619430054289353 1103 18-19 0.3438048967913261 1103 20-21 -0.23188273068433318 1103 22-23 -0.16596413807464927 1103 24-25 0.3597786264030134 1103 26-27 -0.0345814789391099 1103 28-29 0.428013070557796 1103 30-31 0.21565142798532833 1103 32-33 -0.11701347675163731 1103 34-35 0.04924253241544818 1103 36-37 0.32710321441114587 1103 38-39 0.34714596291462385 1103 40-41 0.9724859100252203 1103 42-43 0.8159095749170788 1103 44-45 0.12597740430798154 1103 46-47 0.558805641806913 1103 48-49 0.10310437686695906 1103 50-51 0.04391087871006505 1103 52-53 -0.011865663641451363 1103 54-55 0.836213770168456 1103 56-57 1.1267704178889204 1103 58-59 0.9602611002486299 1103 60-61 1.0811219010728514 1103 62-63 1.5601856513645629 1103 64-65 1.097669478956412 1103 66-67 1.0067957448186853 1103 68-69 1.093379128568678 1103 70-71 1.17255404202281 1103 72-73 1.793550017538287 1103 74-75 2.0422898674050707 1103 76-77 1.9151360895228997 1103 78-79 1.9742051417489783 1103 80-81 1.9240710459567367 1103 82-83 1.5268115577412757 1103 84-85 2.005487284658063 1103 86-87 1.7199123137695302 1103 88-89 1.8474075787806221 1103 90-91 2.294917682329867 1103 92-93 1.732493764413384 1103 94-95 1.1803008814216618 1103 96-97 1.7774201721759475 1103 98-99 2.6868815681342433 1103 100-101 3.2837316277665174 1104 1 0.698456044814769 1104 2 0.5277577278229195 1104 3 1.0056513649809666 1104 4 0.5937232538109924 1104 5 0.836443220020108 1104 6 0.9621068002563646 1104 7 0.8594101726520194 1104 8 0.511766859709951 1104 9 0.936305728759514 1104 10-11 0.40434239479917977 1104 12-13 0.8765214582353167 1104 14-15 1.212976701401864 1104 16-17 1.3806294929475698 1104 18-19 0.7999216195747181 1104 20-21 0.9651991997309253 1104 22-23 1.4094567373462326 1104 24-25 0.9398010730921271 1104 26-27 0.7516138072561773 1104 28-29 0.6726819818933762 1104 30-31 0.6285582742255045 1104 32-33 0.11261615287799032 1104 34-35 -0.05850157532865552 1104 36-37 0.145285032592966 1104 38-39 0.33233114809980435 1104 40-41 0.5237765946492416 1104 42-43 0.32017444584861465 1104 44-45 0.3719931169903674 1104 46-47 0.17676299309760424 1104 48-49 1.0692098763057913 1104 50-51 1.7185461200119718 1104 52-53 1.454015368906255 1104 54-55 1.6825661831875394 1104 56-57 1.5214954459472807 1104 58-59 1.9293969027177624 1104 60-61 1.7117616317125872 1104 62-63 2.034933126112037 1104 64-65 1.601260949214545 1104 66-67 2.386032557388834 1104 68-69 2.6777786796348977 1104 70-71 2.211386814188913 1104 72-73 2.3030898604114647 1104 74-75 2.0347702265520944 1104 76-77 2.141735416122227 1104 78-79 2.892948127158629 1104 80-81 3.332488554374244 1104 82-83 2.590672388268775 1104 84-85 1.7391573183281004 1104 86-87 1.111158105015324 1104 88-89 0.5287768268165358 1104 90-91 1.7532790740245936 1104 92-93 -0.01812351953723379 1104 94-95 0.46806743585488064 1104 96-97 0.8610340891231978 1104 98-99 1.0704954850814907 1104 100-101 2.059489203524091 1105 1 -0.019725773367049726 1105 2 0.15048500055019431 1105 3 -0.04434863501903408 1105 4 -0.13127674618900897 1105 5 0.37053412911101447 1105 6 0.3643795275290955 1105 7 -0.38604437280253023 1105 8 0.01176685970995095 1105 9 -0.21596699851321688 1105 10-11 0.24752421298099136 1105 12-13 0.39243054914441444 1105 14-15 0.6118403377654928 1105 16-17 0.09994767476575817 1105 18-19 0.4635579832110821 1105 20-21 0.5992901088218403 1105 22-23 0.7321840100735031 1105 24-25 0.8693465276375818 1105 26-27 0.35615926180162916 1105 28-29 -0.844363472652077 1105 30-31 0.09219463786187276 1105 32-33 0.03761615287799458 1105 34-35 0.03581660648952578 1105 36-37 -0.36039678558885413 1105 38-39 0.3448311480998072 1105 40-41 0.6726402310128776 1105 42-43 0.44631080948497726 1105 44-45 0.19017493517218753 1105 46-47 0.36312662946123453 1105 48-49 0.8930735126694245 1105 50-51 1.069682483648343 1105 52-53 1.9199244598153342 1105 54-55 1.1939298195511796 1105 56-57 1.2158136277654705 1105 58-59 1.3668969027177624 1105 60-61 1.753807086258039 1105 62-63 1.9212967624756772 1105 64-65 0.9285336764872767 1105 66-67 0.9314871028433735 1105 68-69 1.7686877705439912 1105 70-71 2.500023177825277 1105 72-73 2.722408042229649 1105 74-75 2.0120429538248246 1105 76-77 1.8780990524858616 1105 78-79 1.488402672613173 1105 80-81 2.124534008919696 1105 82-83 2.797490570086957 1105 84-85 0.9948391365099205 1105 86-87 1.6509308322880507 1105 88-89 1.6060495540892603 1105 90-91 0.333960892206413 1105 92-93 0.2443764804627655 1105 94-95 0.9157947085821547 1105 96-97 -1.0321477290586252 1105 98-99 -2.4726863331003273 1105 100-101 -2.0246017055668197 1106 1 0.5802742266329517 1106 2 0.9352676092458445 1106 3 0.918694843241834 1106 4 0.5458971668544663 1106 5 0.38901238998057863 1106 6 0.528509962311702 1106 7 0.20525997502355864 1106 8 0.5074190336229947 1106 9 0.7177286536606999 1106 10-11 0.3855676912418602 1106 12-13 0.47612620131832273 1106 14-15 0.6515142508089724 1106 16-17 0.9472302834614084 1106 18-19 0.1548623310371724 1106 20-21 -0.14962293465642773 1106 22-23 0.5039231405082845 1106 24-25 0.38782478850714597 1106 26-27 0.40181143571467715 1106 28-29 7.452230000950522E-4 1106 30-31 0.6574120291662169 1106 32-33 0.8060944137475587 1106 34-35 0.21027312822865696 1106 36-37 -0.12941852471929138 1106 38-39 0.7932007133171979 1106 40-41 1.2970967527520045 1106 42-43 0.6338108094849773 1106 44-45 0.5227836308243567 1106 46-47 0.3435614120699313 1106 48-49 -1.224861269939268 1106 50-51 -1.148252298960358 1106 52-53 -1.1746407575759648 1106 54-55 -0.9767223543618648 1106 56-57 -0.8010341983214921 1106 58-59 -0.059733532064846884 1106 60-61 0.16848099930152216 1106 62-63 0.7908619798669818 1106 64-65 0.878533676487276 1106 66-67 0.5206175376259843 1106 68-69 0.40564429228312093 1106 70-71 -0.3945420395660264 1106 72-73 -0.30857021863991463 1106 74-75 -0.5814353070447389 1106 76-77 -0.5023357301228337 1106 78-79 -0.9768147186911733 1106 80-81 -0.41731381716725835 1106 82-83 -1.0296833429565204 1106 84-85 -0.05570434175095329 1106 86-87 -0.109395254668474 1106 88-89 -0.17003740243247734 1106 90-91 -0.24158258605445582 1106 92-93 -1.0985583021459284 1106 94-95 -0.5396400740265399 1106 96-97 -0.03323468558036424 1106 98-99 -0.44986024614380327 1106 100-101 0.4286591639984003 1107 1 0.0802742266329517 1107 2 0.18230318236837562 1107 3 -0.2064698471402444 1107 4 0.18160204168977856 1107 5 -0.6544658708889841 1107 6 -0.7106204724709073 1107 7 -0.7769534637116209 1107 8 -0.324596776653685 1107 9 -0.45157305911927637 1107 10-11 -0.004748514291733841 1107 12-13 0.10985479156865097 1107 14-15 0.46146154988671384 1107 16-17 0.5200234323415103 1107 18-19 0.051436771089868216 1107 20-21 0.2591385936703219 1107 22-23 0.876123404012894 1107 24-25 1.033740467031521 1107 26-27 0.31221986786223965 1107 28-29 0.8090456182570165 1107 30-31 0.19825524392248184 1107 32-33 0.6035252437870895 1107 34-35 0.6839226670955867 1107 36-37 1.5240729113808413 1107 38-39 1.4717250874937449 1107 40-41 1.67226144313409 1107 42-43 2.277750203424368 1107 44-45 2.9295688745661224 1107 46-47 2.137369053703658 1107 48-49 3.2540583611542715 1107 50-51 3.4367279381937905 1107 52-53 3.838863853754731 1107 54-55 4.158323758945116 1107 56-57 3.7608893853412226 1107 58-59 2.5960635693844267 1107 60-61 2.4178222377731977 1107 62-63 1.8379634291423415 1107 64-65 1.234594282547885 1107 66-67 0.21330528466155485 1107 68-69 0.20505140690762147 1107 70-71 1.2477504505525516 1107 72-73 1.5909686482902536 1107 74-75 1.2499217417036128 1107 76-77 0.9841596585464671 1107 78-79 0.44446327867378344 1107 80-81 1.084003705889394 1107 82-83 1.7815814791778628 1107 84-85 0.2664300456008242 1107 86-87 -0.019144925287708503 1107 88-89 1.3833222813619876 1107 90-91 0.14115786190338042 1107 92-93 -0.6423659437796552 1107 94-95 0.23473410252154636 1107 96-97 0.7398219679110731 1107 98-99 0.6795863941724001 1107 100-101 1.7837316277665174 1108 1 -1.5447257733670483 1108 2 -0.43701499944980426 1108 3 0.41190136498096663 1108 4 0.7062232538109896 1108 5 -0.1544658708889841 1108 6 -0.3356204724709073 1108 7 -0.3735443728025274 1108 8 -0.1257331402900519 1108 9 0.796533001486786 1108 10-11 0.2850242129809928 1108 12-13 0.42993054914440876 1108 14-15 0.7024653377654957 1108 16-17 0.09057267476575248 1108 18-19 0.38855798321107926 1108 20-21 0.8524151088218375 1108 22-23 0.7634340100735031 1108 24-25 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12-13 0.22592111518214608 2311 14-15 -0.20348985091374772 2311 16-17 0.8379193728789573 2311 18-19 0.7258221341544768 2311 20-21 1.3780636937274977 2311 22-23 0.6142594817716187 2311 24-25 0.7452899238640001 2311 26-27 1.4231403938771052 2311 28-29 1.1049289801781157 2311 30-31 0.8280436944656486 2311 32-33 1.4654463415572394 2311 34-35 1.2082222668668834 2311 36-37 0.8837069879960495 2311 38-39 0.768180204703576 2311 40-41 0.8374987215789176 2311 42-43 1.0724900547679965 2311 44-45 1.188288142719351 2311 46-47 0.2225605917253901 2311 48-49 0.79236596549962 2311 50-51 0.18972965345965775 2311 52-53 0.5015282334002507 2311 54-55 0.45477887615494694 2311 56-57 0.5599174013503756 2311 58-59 1.1275101102649288 2311 60-61 1.0964957655033238 2311 62-63 0.5769571398341711 2311 64-65 1.0907978274306735 2311 66-67 1.6069588009565798 2311 68-69 1.125291544128892 2311 70-71 1.041060913674336 2311 72-73 1.8528325705315325 2311 74-75 2.4243071047682214 2311 76-77 1.4913065996556725 2311 78-79 1.4044404084622322 2311 80-81 1.2360906126932818 2311 82-83 1.5706037776341262 2311 84-85 1.9139429100948213 2311 86-87 1.879940266250312 2311 88-89 1.910766535221338 2311 90-91 2.379008062017732 2311 92-93 1.5177255370665392 2311 94-95 1.6615494255632903 2311 96-97 1.7220975539602463 2311 98-99 2.376841968786465 2311 100-101 2.1013416906595985 2312 1 0.4691631155218374 2312 2 0.593077593142791 2312 3 0.26491062424022616 2312 4 0.9492788093665467 2312 5 0.401089684666573 2312 6 0.5856758238253903 2312 7 0.6287704420122893 2312 8 0.5154705634136505 2312 9 0.9053292977830836 2312 10-11 0.8521538426106261 2312 12-13 0.4692824009962564 2312 14-15 0.22156255998772423 2312 16-17 0.9754106377287144 2312 18-19 1.5598542795073698 2312 20-21 1.4872530717847994 2312 22-23 1.0655173434068317 2312 24-25 1.1869391202301784 2312 26-27 1.2431962988386687 2312 28-29 1.3199883791997706 2312 30-31 1.203305748972987 2312 32-33 0.8089124491742936 2312 34-35 0.1881314213043339 2312 36-37 0.08636247367040539 2312 38-39 0.2452941110627691 2312 40-41 -0.3176375467648995 2312 42-43 -1.1254484497742823 2312 44-45 -1.4727880277907772 2312 46-47 -1.4072437409091307 2312 48-49 -2.3259079688120536 2312 50-51 -2.5178175163516627 2312 52-53 -2.2772977624068815 2312 54-55 -2.2069961063747456 2312 56-57 -1.5244641500123137 2312 58-59 -1.6354179120970542 2312 60-61 -2.5392484692975152 2312 62-63 -2.3379624967835824 2312 64-65 -2.2233181753645788 2312 66-67 -1.9592536378973655 2312 68-69 -2.4127937109374926 2312 70-71 -2.1360879332858325 2312 72-73 -1.7650919577703519 2312 74-75 -1.9268459350640654 2312 76-77 -1.7793083549215432 2312 78-79 -1.7078936236831233 2312 80-81 -1.5111141392284502 2312 82-83 -0.812694615098227 2312 84-85 -0.735253456082674 2312 86-87 -1.215272871415653 2312 88-89 -0.593950445910739 2312 90-91 -1.0785391077935884 2312 92-93 0.06891351749980501 2312 94-95 0.34079470858215544 2312 96-97 0.8854448635339693 2312 98-99 0.6837951483811544 2312 100-101 0.9966945907294793 2313 1 0.09722337917532542 2313 2 0.034383305634943184 2313 3 -0.6384164316292029 2313 4 -0.1825479326296886 2313 5 0.00655107826355561 2313 6 -0.32078996399633297 2313 7 -0.31316301687032677 2313 8 0.34566516479469556 2313 9 0.15140588284271672 2313 10-11 0.07845641637081968 2313 12-13 -0.556298264414913 2313 14-15 -1.1828630520650094 2313 16-17 -0.7375523252342475 2313 18-19 -0.2872894744160348 2313 20-21 -0.0562183657544395 2313 22-23 -0.280527854333279 2313 24-25 0.03502449373927874 2313 26-27 0.3078541770558658 2313 28-29 -0.4852533031605475 2313 30-31 -1.7806867180703279 2313 32-33 -0.9704346945796303 2313 34-35 -0.19871729181555509 2313 36-37 -1.3254391584702105 2313 38-39 -1.2303807163069749 2313 40-41 -2.133080107970166 2313 42-43 -1.0144942752607875 2313 44-45 -1.73863862414985 2313 46-47 -1.3292462518946948 2313 48-49 -2.8147654703814204 2313 50-51 -2.7551056519448807 2313 52-53 -3.4025331673033037 2313 54-55 -3.68784984146577 2313 56-57 -3.022110101048092 2313 58-59 -3.3672132667737635 2313 60-61 -3.318438676453823 2313 62-63 -3.8710761188802536 2313 64-65 -3.201974798088994 2313 66-67 -3.273597642919338 2313 68-69 -3.965210534540759 2313 70-71 -3.575824279801843 2313 72-73 -2.871024161160186 2313 74-75 -3.0972790800734806 2313 76-77 -3.7913924729378685 2313 78-79 -3.737868513827504 2313 80-81 -3.5875422622667408 2313 82-83 -3.8817467180486354 2313 84-85 -3.3121523889138125 2313 86-87 -3.2361454388983937 2313 88-89 -2.759204683198874 2313 90-91 -2.6166747010139275 2313 92-93 -2.845242163605029 2313 94-95 -3.642256138875471 2313 96-97 -3.6096901019399787 2313 98-99 -2.369720231405413 2313 100-101 -1.2529915360752923 2314 1 0.4673710008265033 2314 2 -0.14467628977238434 2314 3 -0.8169292801803252 2314 4 0.1679168021980857 2314 5 0.20037283878843226 2314 6 0.41841178559361225 2314 7 0.007504014294248407 2314 8 0.4246700855163965 2314 9 0.18967816277710625 2314 10-11 0.3918790516906725 2314 12-13 0.5771079684992486 2314 14-15 0.07998549905581598 2314 16-17 -0.23755232523424752 2314 18-19 0.28775153159817535 2314 20-21 0.4275159152734531 2314 22-23 0.19992594555737497 2314 24-25 -0.1637179884914488 2314 26-27 0.47228829405969464 2314 28-29 0.2503945918640511 2314 30-31 -0.12554729762199912 2314 32-33 -0.05189997615426023 2314 34-35 -0.3444253289943475 2314 36-37 0.19000644021759427 2314 38-39 -0.1854107873840647 2314 40-41 0.4762692632709431 2314 42-43 0.14187532561400928 2314 44-45 0.03856203194637686 2314 46-47 0.16473953268704378 2314 48-49 0.010412222346850797 2314 50-51 -0.2516884840935987 2314 52-53 -0.3292690885717562 2314 54-55 -1.071392761093982 2314 56-57 -0.8837831464280832 2314 58-59 -1.0544740650241735 2314 60-61 -2.3224025911613104 2314 62-63 -1.6916064633307748 2314 64-65 -1.64565987189982 2314 66-67 -0.9281903165114649 2314 68-69 -0.9071186810689156 2314 70-71 -1.2023961770134335 2314 72-73 -2.00702744164132 2314 74-75 -1.97344091714292 2314 76-77 -2.0170622378367185 2314 78-79 -1.5567586177094057 2314 80-81 -1.0484498620480451 2314 82-83 -0.9984771718485277 2314 84-85 -0.9249189280062105 2314 86-87 -0.8018917483571109 2314 88-89 -0.714918187846223 2314 90-91 -1.2559584626323002 2314 92-93 -0.8632848098598132 2314 94-95 -0.8285601301275243 2314 96-97 -0.360373535510238 2314 98-99 -0.4670411718100027 2314 100-101 -0.10605331847004251 2315 1 0.3441631155218374 2315 2 0.54215166721686 2315 3 0.3667624760920809 2315 4 0.3520565871443253 2315 5 0.7622007957776802 2315 6 0.7338239719735355 2315 7 0.6334000716419155 2315 8 0.4367668597099481 2315 9 0.6506996681534503 2315 10-11 0.2433575463143285 2315 12-13 -0.021458339744476973 2315 14-15 0.5687847822099386 2315 16-17 0.3388365636546382 2315 18-19 0.9927246498777507 2315 20-21 0.9131789977107232 2315 22-23 1.1210728989623888 2315 24-25 0.5040687498598047 2315 26-27 1.0256037062460734 2315 28-29 0.5723031940145944 2315 30-31 1.58525019341743 2315 32-33 0.6098383751002245 2315 34-35 -0.04335006017714704 2315 36-37 1.0146032144111459 2315 38-39 0.33094225921092146 2315 40-41 0.25180689767955045 2315 42-43 0.5643663650405344 2315 44-45 0.14295271294996326 2315 46-47 0.13673774057235022 2315 48-49 0.4750179571138702 2315 50-51 -0.3580952941294413 2315 52-53 -0.1175755401846601 2315 54-55 0.4480964862178496 2315 56-57 0.4755358499876934 2315 58-59 -0.11226976394890542 2315 60-61 -0.11563735818640097 2315 62-63 0.08101898469789859 2315 64-65 0.10770034315394028 2315 66-67 -0.0587906749344036 2315 68-69 -0.5563122294560117 2315 70-71 -0.6846990443969432 2315 72-73 -1.4942586244370197 2315 74-75 -0.7532348239529547 2315 76-77 -0.8927342808474705 2315 78-79 -1.008819549609047 2315 80-81 -0.13379932441363707 2315 82-83 0.08082390342028845 2315 84-85 0.12817246984324981 2315 86-87 0.12731972117693857 2315 88-89 0.531049554089261 2315 90-91 0.6367386699841902 2315 92-93 0.24020981379610262 2315 94-95 -0.35364973586228743 2315 96-97 -0.10298106239195803 2315 98-99 0.028702555788562734 2315 100-101 1.0128982944331817 2316 1 0.21027422663295425 2316 2 -0.18951499944980554 2316 3 -0.19434863501903266 2316 4 -0.07627674618900926 2316 5 -0.1544658708889841 2316 6 -0.010620472470904474 2316 7 -0.20604437280253052 2316 8 0.3117668597099481 2316 9 -0.020966998513216595 2316 10-11 0.2325242129809908 2316 12-13 0.21743054914440307 2316 14-15 0.20934033776549654 2316 16-17 0.35744767476575134 2316 18-19 0.34355798321107756 2316 20-21 0.18429010882183405 2316 22-23 0.4021840100735048 2316 24-25 0.16934652763758606 2316 26-27 -0.3638407381983697 2316 28-29 0.03813652734792328 2316 30-31 -0.5078053621381287 2316 32-33 -0.3723838471220091 2316 34-35 -1.1716833935104773 2316 36-37 -1.0778967855888553 2316 38-39 -1.14266885190019 2316 40-41 -0.5648597689871266 2316 42-43 -0.8261891905150236 2316 44-45 -1.2048250648278156 2316 46-47 -1.9568733705387658 2316 48-49 -1.184426487330576 2316 50-51 -2.2378175163516616 2316 52-53 -2.3350755401846612 2316 54-55 -2.1710701804488224 2316 56-57 -1.6066863722345346 2316 58-59 -2.1206030972822347 2316 60-61 -2.2136929137419585 2316 62-63 -2.5087032375243226 2316 64-65 -2.036466323512723 2316 66-67 -3.2935128971566243 2316 68-69 -2.3713122294560094 2316 70-71 -2.9049768221747208 2316 72-73 -2.8700919577703523 2316 74-75 -2.9079570461751736 2316 76-77 -2.766900947514138 2316 78-79 -2.3765973273868255 2316 80-81 -1.8529659910803034 2316 82-83 -2.3375094299130446 2316 84-85 -2.0226608634900813 2316 86-87 -2.421569167711951 2316 88-89 -2.6989504459107394 2316 90-91 -3.308539107793589 2316 92-93 -2.4531235195372325 2316 94-95 -2.164205291417847 2316 96-97 -2.672147729058622 2316 98-99 -2.982686333100329 2316 100-101 -2.3996017055668197 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 469.0 3 81.0 4 58.0 5 77.0 6 83.0 7 87.0 8 109.0 9 132.0 10 199.0 11 287.0 12 427.0 13 626.0 14 681.0 15 1026.0 16 1400.0 17 1988.0 18 1751.0 19 1004.0 20 914.0 21 867.0 22 904.0 23 996.0 24 1060.0 25 1116.0 26 1087.0 27 1216.0 28 1301.0 29 1568.0 30 1888.0 31 2265.0 32 3055.0 33 3968.0 34 5530.0 35 8382.0 36 12017.0 37 17688.0 38 15863.0 39 2484.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 45.0042372881356 19.16843220338983 15.564618644067796 20.26271186440678 2 14.915476846163623 20.792907681226964 41.99678007033004 22.29483540227937 3 18.727428698516253 27.00401385255711 31.147072217574106 23.12148523135253 4 11.422459439996612 16.887999322234933 38.95348837209303 32.73605286567543 5 11.880380799085067 38.39547615769911 34.29733250029121 15.426810542924613 6 28.309337285623542 37.81812407368198 18.021384713106077 15.851153927588395 7 25.425810071241813 35.7691045550298 21.73669111965025 17.068394254078143 8 25.52268035780448 33.886624675805855 20.530355157995025 20.06033980839464 9 26.192593533633996 16.620085117194943 21.924029727497935 35.263291621673126 10-11 23.751925509896193 27.740046900392244 27.010073526687172 21.497954063024387 12-13 25.745048011264387 25.87579533544364 28.57543644197889 19.803720211313085 14-15 22.128474942304845 26.05439224238318 29.254620905761048 22.56251190955093 16-17 18.98239058290505 30.18742093758105 29.420482393229346 21.409706086284555 18-19 18.825528401060772 26.88294983564385 34.824977900581736 19.46654386271365 20-21 19.969726800249806 25.770325913223886 34.95072666264436 19.309220623881956 22-23 19.41718797963439 26.15509193102791 35.03858245212918 19.389137637208513 24-25 19.23048425509394 25.917967716327073 35.30934109552792 19.54220693305107 26-27 18.730483015592416 26.37161396860346 35.4952418253607 19.402661190443425 28-29 18.435177539223783 26.844735226238114 35.45702852061234 19.263058713925766 30-31 18.53061354109328 26.634945166617268 35.27173222678579 19.562709065503665 32-33 18.419478629085617 26.86780468439857 35.072647876141325 19.640068810374487 34-35 18.994235990917318 26.709221885238527 34.85822866549164 19.43831345835252 36-37 19.003868727871247 26.56773449201116 35.00907642722188 19.419320352895724 38-39 18.61533494582965 27.164564599531072 34.76323296690501 19.456867487734268 40-41 18.602940398192153 27.277009219175035 34.704213723975144 19.41583665865766 42-43 18.605377368476766 27.226103524928547 33.95787022335133 20.21064888324336 44-45 18.648750205079622 27.461087795248503 33.72514567268763 20.165016326984244 46-47 19.221588894357737 27.51589053130739 33.23383558526375 20.028684989071124 48-49 19.265355810199274 27.602614655834017 32.56516791489136 20.566861619075343 50-51 19.005499711516578 27.906668995707157 32.820944425917986 20.266886866858282 52-53 19.433710577299724 28.381168150587037 31.77689318949363 20.408228082619605 54-55 18.905501600995 27.769456720209583 32.20513906163169 21.119902617163724 56-57 18.999439076275042 27.981097928815608 31.968418935938274 21.051044058971076 58-59 19.625213668428934 27.324156836595908 32.1410238091458 20.90960568582935 60-61 19.11883781852822 27.09216480114313 33.06078166759281 20.728215712735835 62-63 18.763495490918327 26.771878572335833 33.276705194970155 21.18792074177569 64-65 18.76514854838539 26.587356188016635 33.62210391507287 21.025391348525098 66-67 18.78731106312714 26.51467039248063 33.07718362335408 21.62083492103815 68-69 18.541080085526175 26.16645849651756 33.04348286300993 22.248978554946333 70-71 18.72807040755304 26.206491423974764 32.95088300512815 22.11455516334404 72-73 18.5800852054722 25.79132597708449 33.41112963403985 22.217459183403456 74-75 18.277469924792133 26.30475857798384 32.88293294803194 22.534838549192084 76-77 18.111086411127694 26.021256100013762 33.00517641081012 22.86248107804842 78-79 18.406051014412974 25.929591852937904 32.7904428694681 22.873914263181018 80-81 18.072837552463465 25.683149766329173 32.84305682726354 23.400955853943824 82-83 18.367400946342187 25.18656913908266 32.969016291058445 23.47701362351671 84-85 18.3626808084978 25.138798473613733 32.72256712341817 23.775953594470288 86-87 18.336985170366134 24.9198183608015 32.69823124067194 24.04496522816043 88-89 18.031945297118785 24.489806719309016 32.784152253530074 24.69409573004213 90-91 18.00879639675878 24.8612000571607 32.99866095765345 24.131342588427074 92-93 17.90363894239929 25.441372594678125 32.68538707423951 23.969601388683078 94-95 17.762618217228624 25.724386487645074 31.8068514392468 24.706143855879503 96-97 17.842154564265943 26.227660261329298 31.535746227977796 24.394438946426966 98-99 18.613327970532822 26.226740616863186 30.665869302906497 24.494062109697495 100-101 19.100136535388067 25.481842910214752 30.836887839883154 24.581132714514027 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 193.0 1 126.0 2 77.5 3 101.5 4 78.5 5 63.5 6 122.0 7 266.5 8 463.0 9 548.0 10 437.0 11 338.0 12 351.0 13 370.5 14 371.5 15 374.0 16 387.5 17 400.5 18 420.0 19 437.0 20 467.0 21 534.5 22 588.0 23 657.0 24 782.0 25 884.5 26 1018.0 27 1177.0 28 1293.0 29 1393.5 30 1526.0 31 1676.5 32 1816.0 33 2032.5 34 2212.5 35 2327.0 36 2505.5 37 2622.5 38 2727.5 39 2834.0 40 2869.0 41 2907.0 42 3045.5 43 3200.5 44 3221.0 45 3235.0 46 3269.5 47 3481.0 48 3867.0 49 3754.5 50 3182.0 51 2790.5 52 2631.5 53 2505.0 54 2285.5 55 2011.0 56 1793.0 57 1543.0 58 1293.5 59 1114.0 60 907.0 61 703.0 62 557.5 63 426.0 64 298.5 65 227.0 66 168.0 67 114.5 68 86.0 69 67.5 70 51.5 71 32.0 72 21.0 73 19.0 74 20.0 75 17.0 76 8.5 77 4.0 78 3.0 79 5.0 80 3.0 81 3.0 82 3.0 83 0.5 84 0.5 85 2.0 86 2.0 87 0.5 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.26834576457413317 2 0.2556680119170875 3 0.24404673864812898 4 0.23876434170769328 5 0.2334819447672576 6 0.20495700128890484 7 0.19756164557229489 8 0.19967460434846918 9 0.20706996006507913 10-11 0.20971115853529698 12-13 0.20812643945316628 14-15 0.20284404251273055 16-17 0.19808988526633844 18-19 0.20548524098294843 20-21 0.19122276924377204 22-23 0.19122276924377204 24-25 0.18910981046759778 26-27 0.1954486867961206 28-29 0.20706996006507913 30-31 0.19650516618420774 32-33 0.202315802818687 34-35 0.1991463646544256 36-37 0.18858157077355422 38-39 0.19386396771398992 40-41 0.1869968516914235 42-43 0.19439220740803345 44-45 0.18858157077355422 46-47 0.18963805016164134 48-49 0.1970334058782513 50-51 0.20548524098294843 52-53 0.20812643945316628 54-55 0.19175100893781563 56-57 0.1764320578105521 58-59 0.18329917383311853 60-61 0.18646861199737993 62-63 0.18805333107951064 64-65 0.18277093413907494 66-67 0.18805333107951064 68-69 0.1901662898556849 70-71 0.1854121326092928 72-73 0.18646861199737993 74-75 0.19280748832590278 76-77 0.19756164557229489 78-79 0.20125932343059988 80-81 0.19386396771398992 82-83 0.1954486867961206 84-85 0.19280748832590278 86-87 0.19122276924377204 88-89 0.1901662898556849 90-91 0.19386396771398992 92-93 0.18594037230333635 94-95 0.18752509138546708 96-97 0.1854121326092928 98-99 0.18594037230333635 100-101 0.18277093413907494 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 94654.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 76.03376508124326 #Duplication Level Percentage of deduplicated Percentage of total 1 90.10268309966791 68.50846239989858 2 6.030374188886882 9.170241088596361 3 1.6826689268990815 3.838189616920574 4 0.7280912615153747 2.214380797430642 5 0.41962511636954797 1.595283876011579 6 0.27233947949811727 1.2424197603904747 7 0.15840153399380288 0.8430705516935364 8 0.10976948408342481 0.6676949732710715 9 0.09448512554016313 0.6465653855093287 >10 0.36126665647709433 4.71823694719716 >50 0.019452819964151234 0.9476620111141633 >100 0.0166738456835582 2.229171508863862 >500 0.0013894871402965166 0.7913030616772667 >1k 0.002778974280593033 2.587318021425402 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1396 1.4748452257696454 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1053 1.1124727956557567 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 749 0.7913030616772667 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 248 0.2620068882456103 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 233 0.24615969742430324 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 229 0.2419337798719547 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 213 0.22503010966256048 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 196 0.20706996006507913 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 191 0.2017875631246434 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 179 0.18910981046759778 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 139 0.14685063494411224 No Hit NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 131 0.13839879983941514 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 131 0.13839879983941514 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 116 0.12255160901810805 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 104 0.10987385636106241 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 96 0.10142202125636528 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.0010564793880871385 0.0 24-25 0.0 0.0 0.0 0.002112958776174277 0.0 26-27 0.0 0.0 0.0 0.0036976778583049847 0.0 28-29 0.0 0.0 0.0 0.008451835104697106 0.0 30-31 0.0 0.0 0.0 0.02218606714982991 0.0 32-33 0.0 0.0 0.0 0.040674456441354825 0.0 34-35 0.0 0.0 0.0 0.050711010628182646 0.0 36-37 0.0 0.0 0.0 0.06391700297927189 0.0 38-39 0.0 0.0 0.0 0.09508314492784245 0.0 40-41 0.0 0.0 0.0 0.17960149597481354 0.0 42-43 0.0 0.0 0.0 0.3084919813214444 0.0 44-45 0.0 0.0 0.0 0.43315654911572676 0.0 46-47 0.0 0.0 0.0 0.7432332495193019 0.0 48-49 0.0 0.0 0.0 1.0723265789084455 0.0 50-51 0.0 0.0 0.0 1.282565977137786 0.0 52-53 0.0 0.0 0.0 1.5620047752868342 0.0 54-55 0.0 0.0 0.0 1.8409153337418387 0.0 56-57 0.0 0.0 0.0 2.5096667864009974 0.0 58-59 0.0 0.0 0.0 3.2375810847930357 0.0 60-61 0.0 0.0 0.0 3.9871532106408605 0.0 62-63 0.0 0.0 0.0 4.903120840112409 0.0 64-65 0.0 0.0 0.0 5.842331016121875 0.0 66-67 0.0 0.0 0.0 6.897753924820927 0.0 68-69 0.0 0.0 0.0 8.018678555581381 0.0 70-71 0.0 0.0 0.0 9.120586557356265 0.0 72-73 0.0 0.0 0.0 10.2267204766835 0.0 74-75 0.0 0.0 0.0 11.333910875398821 0.0 76-77 0.0 0.0 0.0 12.789739472182898 0.0 78-79 0.0 0.0 0.0 13.971939907452406 0.0 80-81 0.0 0.0 0.0 15.490100788133624 0.0 82-83 0.0 0.0 0.0 17.27924863185919 0.0 84-85 0.0 0.0 0.0 19.35734358822659 0.0 86-87 0.0 0.0 0.0 21.130644241130856 0.0 88-89 0.0 0.0 0.0 22.94726054894669 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TAGTAGC 15 6.595147E-4 95.01378 2 CCATACT 30 1.3564386E-6 79.17815 4 CCCATAC 20 0.0020669736 71.26033 3 GTTAGAG 30 1.2941414E-4 63.342518 2 GTAGTAG 25 0.004983189 57.068752 1 GGTTAGA 35 2.7583272E-4 54.351196 1 ATTGGCG 20 5.3728267E-4 47.506886 16-17 TTAGAGC 45 9.5733133E-4 42.228344 3 GGTATCA 490 0.0 39.79284 1 ATTACGA 25 0.0016112461 38.005512 34-35 GCGGCGC 25 0.0016112461 38.005512 20-21 GTATCAA 770 0.0 36.440002 1 ATGGGAT 165 1.8189894E-12 34.55047 5 TAGAGCC 55 0.0025658666 34.550465 4 GTACAGT 70 2.0373936E-4 33.933495 6 ATGTACA 85 1.61484E-5 33.534275 4 ATGGGGA 60 0.003929965 31.671259 5 ATGGGAG 120 1.4792022E-7 31.671259 7 CGGCGCT 30 0.003939863 31.671259 22-23 CTCATAA 30 0.003939863 31.671259 50-51 >>END_MODULE