FastQCFastQC Report
Thu 26 May 2016
SRR937693_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937693_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences94654
Sequences flagged as poor quality0
Sequence length101
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10091.0659877025799227No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8880.9381536966213789No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6450.6814292053162043No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT5880.6212098801952374No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4650.4912629154605194No Hit
GTCCCAGGGTGTGCTTGTCAAAGAGATATTCTGCCATGCCAGCTTCAGGG2260.23876434170769328No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2160.22819954782682192No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1890.19967460434846918No Hit
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA1620.17114966087011643No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT1210.12783400595854374No Hit
CTACTGGAACTGCACAAACTGGCTACTGACAAGAATGATCCCCACTTATG1070.11304329452532381No Hit
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT1040.10987385636106241No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTAGCT156.587489E-495.0417568
TCGTAGC200.002064578271.281327
TTCGTAG250.00500885256.994936
TATTCGT250.00500885256.994934
ATATTCG250.00500885256.994933
GTCCCAG801.0639915E-535.640661
TGGGGAG550.002562198334.560648
TACACTG550.00256885334.542385
GGTGTGC851.6116343E-533.5441478
GTGTGCT902.3826384E-531.6805849
GAATGCG300.003944410531.66384782-83
CTATTGT600.003934533331.6638474
GGTATCA11650.029.3691251
GGGAATC650.005804877329.2436169
ACTGTGC650.005804877329.2436168
GAACTGC1004.8911952E-528.5125267
GACAGCT700.00833549227.1547897
TAGAACA700.00835702727.1404444
TCTTATC350.00837781827.14044494-95
TACCTGG700.00835702727.1404442
Produced by FastQC (version 0.11.5)