##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937693_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 94654 Sequences flagged as poor quality 0 Sequence length 101 %GC 41 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.33246349863714 33.0 31.0 34.0 31.0 34.0 2 32.513533500961394 34.0 31.0 34.0 31.0 34.0 3 32.55727174762821 34.0 31.0 34.0 31.0 34.0 4 36.149893295581805 37.0 35.0 37.0 35.0 37.0 5 36.1032814249794 37.0 35.0 37.0 35.0 37.0 6 36.17908382107465 37.0 36.0 37.0 35.0 37.0 7 36.13119361041266 37.0 35.0 37.0 35.0 37.0 8 36.111215585183935 37.0 35.0 37.0 35.0 37.0 9 37.79208485642445 39.0 38.0 39.0 35.0 39.0 10-11 37.710614448412116 39.0 37.0 39.0 35.0 39.0 12-13 37.623687324360304 39.0 37.0 39.0 35.0 39.0 14-15 38.7771462378769 40.0 38.0 41.0 35.0 41.0 16-17 38.76933885519894 40.0 38.0 41.0 34.5 41.0 18-19 38.79474718448243 40.0 38.0 41.0 35.0 41.0 20-21 38.74338643903057 40.0 38.0 41.0 35.0 41.0 22-23 38.67699199188624 40.0 38.0 41.0 35.0 41.0 24-25 38.6425243518499 40.0 38.0 41.0 34.0 41.0 26-27 38.41357470365753 40.0 38.0 41.0 34.0 41.0 28-29 38.35708475077651 40.0 38.0 41.0 34.0 41.0 30-31 38.26539290468443 40.0 37.5 41.0 34.0 41.0 32-33 38.01647051366028 40.0 37.0 41.0 34.0 41.0 34-35 37.782259598115246 40.0 36.0 41.0 33.0 41.0 36-37 37.55007184059839 40.0 36.0 41.0 33.0 41.0 38-39 37.31712870031906 40.0 36.0 41.0 33.0 41.0 40-41 37.040790669174044 40.0 35.0 41.0 32.5 41.0 42-43 36.70110085152238 40.0 35.0 41.0 31.5 41.0 44-45 36.16118706024045 39.0 35.0 41.0 30.0 41.0 46-47 35.52346968960636 39.0 35.0 41.0 27.5 41.0 48-49 34.93880343144505 39.0 35.0 41.0 23.5 41.0 50-51 34.53270860185518 39.0 35.0 40.5 21.0 41.0 52-53 34.211586409449154 39.0 35.0 40.0 17.5 41.0 54-55 33.94521626133074 38.0 34.0 40.0 14.0 41.0 56-57 33.57907219980139 38.0 34.0 40.0 7.5 41.0 58-59 33.27743676970863 38.0 34.0 40.0 4.0 41.0 60-61 32.87007944724998 37.5 33.0 40.0 2.0 41.0 62-63 32.433600270458726 37.0 33.0 40.0 2.0 41.0 64-65 32.00740063811355 36.0 32.0 39.5 2.0 41.0 66-67 31.546463963488073 36.0 31.5 39.0 2.0 40.5 68-69 31.001352293616755 35.0 31.0 39.0 2.0 40.0 70-71 30.51746888668202 35.0 30.5 38.0 2.0 40.0 72-73 30.04554482642044 35.0 30.0 37.5 2.0 40.0 74-75 29.569252223889112 35.0 29.0 37.0 2.0 39.0 76-77 28.388256175122024 33.5 28.0 35.5 2.0 38.5 78-79 28.888087138419927 34.0 29.0 36.0 2.0 39.0 80-81 28.68452997232024 34.0 29.0 36.0 2.0 37.5 82-83 28.300737422612883 34.0 29.0 35.0 2.0 37.0 84-85 27.908149681999703 34.0 28.5 35.0 2.0 37.0 86-87 27.62436875356562 34.0 27.0 35.0 2.0 36.0 88-89 27.255292961734316 34.0 26.5 35.0 2.0 36.0 90-91 26.796231538022695 33.5 25.5 35.0 2.0 35.0 92-93 26.401890041625286 33.0 25.0 35.0 2.0 35.0 94-95 25.96411139518668 33.0 23.5 35.0 2.0 35.0 96-97 25.508039808143344 33.0 21.5 35.0 2.0 35.0 98-99 24.821211993154016 32.5 10.0 34.5 2.0 35.0 100-101 22.74366112367148 30.5 2.0 33.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.4787487626915663 1101 2 0.3162361765569415 1101 3 0.4010563298201504 1101 4 0.3891319244482645 1101 5 0.5033012864558302 1101 6 0.36559448965529384 1101 7 0.42677301626161324 1101 8 0.367332313374348 1101 9 0.2951358748676256 1101 10-11 0.48849251515659375 1101 12-13 0.5737049801669585 1101 14-15 0.7882074057718214 1101 16-17 0.850079776166055 1101 18-19 0.7976422804221457 1101 20-21 0.5790588963701921 1101 22-23 0.6060161074933035 1101 24-25 0.5684448204669579 1101 26-27 0.8303485982925238 1101 28-29 1.0660563336434095 1101 30-31 0.9695655870703419 1101 32-33 0.946847899871635 1101 34-35 1.1603177115359173 1101 36-37 1.336266843694034 1101 38-39 1.0900385440810751 1101 40-41 1.284318183512383 1101 42-43 1.4804596441321678 1101 44-45 1.912032479799663 1101 46-47 1.8702319602586215 1101 48-49 1.8732419983801307 1101 50-51 1.7791402850999773 1101 52-53 1.8517509110743475 1101 54-55 1.859811032322476 1101 56-57 2.168212039147903 1101 58-59 2.1812144422999395 1101 60-61 2.6833895177594727 1101 62-63 2.523419338249088 1101 64-65 2.6786618462252143 1101 66-67 3.1375598922383183 1101 68-69 3.143926088981541 1101 70-71 2.9128154599485754 1101 72-73 2.975184897179176 1101 74-75 3.1691997969919576 1101 76-77 2.4452344836629862 1101 78-79 3.0006867091952927 1101 80-81 2.64106895056533 1101 82-83 3.271470078429772 1101 84-85 2.9959227751560995 1101 86-87 3.0031716714675127 1101 88-89 2.219959197585524 1101 90-91 2.5512386064170833 1101 92-93 2.8508711428280478 1101 94-95 2.8455173992059137 1101 96-97 2.6539298723353646 1101 98-99 2.6467446452571863 1101 100-101 2.981976255812544 1102 1 0.2947487626915688 1102 2 0.3149028432236136 1102 3 0.2970563298201512 1102 4 0.24513192444825904 1102 5 0.18063461978916706 1102 6 0.2562611563219619 1102 7 0.2894396829282755 1102 8 -0.02333435329232003 1102 9 0.2298025415342977 1102 10-11 0.015159181823257484 1102 12-13 0.33503831350029856 1102 14-15 0.3108740724384873 1102 16-17 0.13074644283271653 1102 18-19 0.2596422804221419 1102 20-21 0.28039222970352995 1102 22-23 0.22134944082663566 1102 24-25 0.1304448204669626 1102 26-27 0.1230152649591929 1102 28-29 0.4427230003100746 1102 30-31 0.4948989204036778 1102 32-33 0.6361812332049652 1102 34-35 1.0003177115359208 1102 36-37 0.5336001770273668 1102 38-39 0.8207052107477395 1102 40-41 1.170984850179046 1102 42-43 1.313126310798836 1102 44-45 1.153365813132993 1102 46-47 1.422231960258621 1102 48-49 1.8259086650467964 1102 50-51 2.061140285099974 1102 52-53 1.7950842444076827 1102 54-55 2.421144365655806 1102 56-57 2.9242120391479034 1102 58-59 2.5345477756332784 1102 60-61 2.517389517759476 1102 62-63 2.564752671582422 1102 64-65 2.8706618462252145 1102 66-67 2.827559892238316 1102 68-69 2.9999260889815353 1102 70-71 2.7681487932819095 1102 72-73 3.309184897179179 1102 74-75 3.310533130325286 1102 76-77 3.201234483662983 1102 78-79 3.5020200425286276 1102 80-81 3.357735617231995 1102 82-83 3.34813674509644 1102 84-85 3.8065894418227657 1102 86-87 3.193171671467514 1102 88-89 3.065959197585528 1102 90-91 3.0739052730837493 1102 92-93 2.845537809494715 1102 94-95 2.7488507325392497 1102 96-97 2.7272632056686987 1102 98-99 2.4200779785905198 1102 100-101 3.5053095891458774 1103 1 0.5212178984940365 1103 2 0.34068062100138974 1103 3 0.15068595944977403 1103 4 0.28888501086801455 1103 5 0.151202521023734 1103 6 0.26732288471702503 1103 7 0.20973597922457543 1103 8 -0.054642995267627725 1103 9 0.18476550449725693 1103 10-11 0.14083819416894272 1103 12-13 -0.2132086000799518 1103 14-15 -0.2759901250923704 1103 16-17 0.0768698996228494 1103 18-19 0.34344474955794624 1103 20-21 0.3202934642714226 1103 22-23 0.6024358605797246 1103 24-25 0.6359756846644942 1103 26-27 0.3645461291567216 1103 28-29 0.491118062038467 1103 30-31 0.5912939821320649 1103 32-33 0.4967244430815114 1103 34-35 0.5346386991902392 1103 36-37 0.6081186955458833 1103 38-39 0.8690508897600893 1103 40-41 0.6862934921543555 1103 42-43 0.9874966811692047 1103 44-45 1.185118899552748 1103 46-47 0.5151702318635571 1103 48-49 0.22768644282457728 1103 50-51 1.2595106554703435 1103 52-53 0.8461953555187947 1103 54-55 1.187465353310131 1103 56-57 1.5872243848269108 1103 58-59 2.087881108966613 1103 60-61 1.5573401350434253 1103 62-63 1.1752711901009398 1103 64-65 1.2376741919042296 1103 66-67 1.448794460139549 1103 68-69 1.1700989284877075 1103 70-71 1.046519163652281 1103 72-73 0.6096293416236165 1103 74-75 1.2012985624240535 1103 76-77 0.9333826318111349 1103 78-79 0.6905632524051697 1103 80-81 0.6814393209356986 1103 82-83 1.008383658676685 1103 84-85 0.6823425282425184 1103 86-87 1.3865050048008492 1103 88-89 1.29119376548676 1103 90-91 1.3087694706146138 1103 92-93 2.0234637354206377 1103 94-95 1.8051470288355453 1103 96-97 2.2050409834464766 1103 98-99 1.408966867479407 1103 100-101 1.591729342232295 1104 1 0.42783967178247906 1104 2 0.39926647958724715 1104 3 0.7937836025474212 1104 4 0.5418591971755333 1104 5 0.35299825615280156 1104 6 0.2904429745037831 1104 7 0.4770760465646404 1104 8 0.520665646707684 1104 9 0.5854389051706619 1104 10-11 0.39515918182326004 1104 12-13 0.8579474044093871 1104 14-15 0.4457831633475777 1104 16-17 0.41401917010544764 1104 18-19 0.6167331895130488 1104 20-21 0.5433013206126134 1104 22-23 0.6948039862811797 1104 24-25 1.002081184103325 1104 26-27 0.5033789013228258 1104 28-29 1.0081775457646245 1104 30-31 0.999262556767313 1104 32-33 1.465635778659511 1104 34-35 1.1166813478995508 1104 36-37 1.7168729043000894 1104 38-39 1.6930688471113768 1104 40-41 1.6473484865426826 1104 42-43 1.2262172198897474 1104 44-45 1.7744567222239027 1104 46-47 2.2556865057131645 1104 48-49 2.8408177559558894 1104 50-51 3.216413012372705 1104 52-53 3.780538789862227 1104 54-55 3.714962547473995 1104 56-57 3.5354847664206233 1104 58-59 3.490911411996912 1104 60-61 3.8642986086685696 1104 62-63 3.2385708534005992 1104 64-65 2.731389118952489 1104 66-67 2.011196255874676 1104 68-69 2.100289725345174 1104 70-71 2.457966975100092 1104 72-73 2.092457624451903 1104 74-75 1.793442221234379 1104 76-77 1.7670526654811702 1104 78-79 1.5594745879831677 1104 80-81 1.859553799050179 1104 82-83 1.7281367450964389 1104 84-85 2.0898621690954933 1104 86-87 2.9622625805584235 1104 88-89 3.1899591975855266 1104 90-91 2.405178000356475 1104 92-93 2.724810536767439 1104 94-95 3.3633961870846996 1104 96-97 3.245445023850518 1104 98-99 3.9473507058632507 1104 100-101 4.559855043691332 1105 1 -0.23125123730843455 1105 2 0.06290284322361117 1105 3 -0.4539436701798536 1105 4 -0.23086807555174005 1105 5 0.016634619789165583 1105 6 0.07226115632196439 1105 7 0.3134396829282764 1105 8 -0.3293343532923174 1105 9 0.5218025415342922 1105 10-11 0.14515918182326004 1105 12-13 0.029538313500296454 1105 14-15 -0.0326259275615044 1105 16-17 -0.025753557167277563 1105 18-19 -0.3423577195778549 1105 20-21 -1.422607770296473 1105 22-23 -0.9131505591733671 1105 24-25 -0.7990551795330347 1105 26-27 -0.48298473504080874 1105 28-29 -0.1372769996899308 1105 30-31 -0.11210107959632154 1105 32-33 0.35768123320496414 1105 34-35 0.6053177115359105 1105 36-37 0.9021001770273642 1105 38-39 0.8067052107477437 1105 40-41 -0.2640151498209562 1105 42-43 0.8296263107988437 1105 44-45 1.002865813132992 1105 46-47 1.3602319602586235 1105 48-49 1.8374086650468016 1105 50-51 2.501640285099974 1105 52-53 2.1600842444076775 1105 54-55 1.8081443656558065 1105 56-57 2.658212039147898 1105 58-59 2.6295477756332772 1105 60-61 3.1858895177594704 1105 62-63 1.7567526715824222 1105 64-65 2.6336618462252197 1105 66-67 2.660059892238312 1105 68-69 2.101426088981537 1105 70-71 2.401148793281912 1105 72-73 2.015184897179175 1105 74-75 2.0650331303252862 1105 76-77 1.9352344836629847 1105 78-79 2.364020042528626 1105 80-81 2.727735617231996 1105 82-83 2.7781367450964396 1105 84-85 2.975089441822764 1105 86-87 3.1906716714675127 1105 88-89 2.464959197585525 1105 90-91 2.0654052730837513 1105 92-93 2.1975378094947153 1105 94-95 2.6338507325392477 1105 96-97 3.552263205668698 1105 98-99 3.8825779785905183 1105 100-101 3.1303095891458774 1106 1 -0.5855990633953887 1106 2 -0.32840150460247486 1106 3 -0.5006828006146335 1106 4 -0.5004332929430433 1106 5 -0.47466972803692187 1106 6 -1.0407823219389059 1106 7 -0.10829944750650355 1106 8 -0.7532473967705755 1106 9 -0.3651539802048376 1106 10-11 -0.9222321225245693 1106 12-13 -0.6547008169344863 1106 14-15 -1.2233867971267287 1106 16-17 -1.3328187745585822 1106 18-19 -1.0097490239256857 1106 20-21 -1.030216465948648 1106 22-23 -1.1180418635211922 1106 24-25 -1.7789464838808655 1106 26-27 -1.5938543002582009 1106 28-29 -1.6068422170812298 1106 30-31 -1.821883688291983 1106 32-33 -2.2493839841863377 1106 34-35 -2.3805518536814745 1106 36-37 -2.616921562103073 1106 38-39 -2.6976426153392126 1106 40-41 -1.6694499324296501 1106 42-43 -1.3937432544185526 1106 44-45 -2.9737646216496145 1106 46-47 -2.491941952784856 1106 48-49 -1.843569595822764 1106 50-51 -2.7630336279435 1106 52-53 -1.1018722773314522 1106 54-55 -2.3798991126050595 1106 56-57 -3.4722227434607973 1106 58-59 -5.7889304852362855 1106 60-61 -5.041827873544875 1106 62-63 -5.8867255892871455 1106 64-65 -6.1804685885573925 1106 66-67 -4.843744455587771 1106 68-69 -5.89042173710542 1106 70-71 -6.5629816415006985 1106 72-73 -6.280467276733869 1106 74-75 -6.538771217500798 1106 76-77 -6.225635081554405 1106 78-79 -5.359893000949636 1106 80-81 -6.196177426246265 1106 82-83 -7.267515428816605 1106 84-85 -7.096106210351149 1106 86-87 -7.29465441548901 1106 88-89 -6.979606019805779 1106 90-91 -6.461225161698859 1106 92-93 -6.369853494853114 1106 94-95 -7.66506231093901 1106 96-97 -8.13795418563565 1106 98-99 -7.697313325757307 1106 100-101 -7.54903823694108 1107 1 -0.4024633585205528 1107 2 -0.31588503556426417 1107 3 -0.6001557913919733 1107 4 -0.13086807555173863 1107 5 -0.08336538021083584 1107 6 -0.03682975276894496 1107 7 -0.20777243828384684 1107 8 -0.018728292686255088 1107 9 0.009681329413083972 1107 10-11 0.08303796970204758 1107 12-13 0.20037164683362363 1107 14-15 0.2548740724384899 1107 16-17 -0.21325355716727756 1107 18-19 -0.1408425680627019 1107 20-21 -0.06578958847828886 1107 22-23 0.5766221680993695 1107 24-25 -0.07670669468455316 1107 26-27 -1.1360150380711147 1107 28-29 -0.6766709390838628 1107 30-31 -0.4704344129296558 1107 32-33 -0.3192127061889707 1107 34-35 -0.33180350058529484 1107 36-37 -0.07706648963930007 1107 38-39 -0.46147660743407926 1107 40-41 -0.09204545285125221 1107 42-43 0.46561115928368224 1107 44-45 -0.17402812626094288 1107 46-47 -1.1352225851959261 1107 48-49 -0.3682731531350143 1107 50-51 0.23762513358482096 1107 52-53 -0.8103703010468664 1107 54-55 0.2149625474739878 1107 56-57 -0.7584546275187662 1107 58-59 -0.7606037395182383 1107 60-61 -0.1660044216344616 1107 62-63 0.36887388370363894 1107 64-65 1.2404800280433967 1107 66-67 0.7445295892080139 1107 68-69 0.31544124049668554 1107 70-71 0.4034215205546374 1107 72-73 0.725790957785236 1107 74-75 0.11768464547680324 1107 76-77 -0.29052309209458826 1107 78-79 1.1776564061649921 1107 80-81 1.5686447081410861 1107 82-83 1.3069246238843135 1107 84-85 1.6868318660651873 1107 86-87 1.2652928835887245 1107 88-89 1.7838985915249168 1107 90-91 0.1263901215686012 1107 92-93 0.4763256882825928 1107 94-95 1.3421840658725834 1107 96-97 1.2908995693050613 1107 98-99 0.7321991907117322 1107 100-101 0.8689459527822407 1108 1 -0.2812512373084317 1108 2 0.28165284322361117 1108 3 -0.7601936701798522 1108 4 -0.5371180755517386 1108 5 -0.08336538021083584 1108 6 0.24726115632196155 1108 7 0.3509396829282778 1108 8 -0.17308435329231742 1108 9 -0.18444745846570498 1108 10-11 -0.2829658181767414 1108 12-13 -0.28921168649970497 1108 14-15 -0.3545009275615101 1108 16-17 -0.6195035571672776 1108 18-19 -0.16735771957785772 1108 20-21 0.14301722970352415 1108 22-23 0.28684944082663577 1108 24-25 -0.4053051795330376 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7 0.08325100368299587 2311 8 -0.09820227782061863 2311 9 0.27840631511919867 2311 10-11 0.5593101252194899 2311 12-13 0.24439680406633357 2311 14-15 0.09449671394792603 2311 16-17 0.2961804050968766 2311 18-19 0.009529072874975952 2311 20-21 0.3561658146091844 2311 22-23 0.34934944082663577 2311 24-25 -0.07098914179719173 2311 26-27 -0.13487152749364384 2311 28-29 0.11177960408366516 2311 30-31 -0.13710107959632722 2311 32-33 0.3465963275445887 2311 34-35 0.09729884361139085 2311 36-37 0.985826592121704 2311 38-39 0.46991275791754816 2311 40-41 0.937399944518674 2311 42-43 1.702031971176197 2311 44-45 1.6710261904914887 2311 46-47 2.3819300734661724 2311 48-49 3.289531306556235 2311 50-51 3.34197047377922 2311 52-53 3.7256502821435262 2311 54-55 2.5142764411274996 2311 56-57 2.917646001412052 2311 58-59 2.2828496624257326 2311 60-61 3.009238574363252 2311 62-63 1.581280973469216 2311 64-65 2.6907373179233325 2311 66-67 2.9249183828043535 2311 68-69 2.5695864663400236 2311 70-71 3.36482803856493 2311 72-73 4.438769802839552 2311 74-75 3.5176274699479286 2311 76-77 3.124857125172422 2311 78-79 2.2857181557361734 2311 80-81 1.9022639191187913 2311 82-83 2.5602122167945502 2311 84-85 2.404098875785028 2311 86-87 2.543737709203363 2311 88-89 1.5031667447553367 2311 90-91 2.466584518366769 2311 92-93 2.5598019604381115 2311 94-95 3.4239450721618887 2311 96-97 3.342357545291339 2311 98-99 3.218191186137691 2311 100-101 4.599649211787387 2312 1 0.40393394787675163 2312 2 0.5629028432236112 2312 3 0.7247600335238502 2312 4 0.27653933185566615 2312 5 0.23144943460398082 2312 6 0.3907796748404806 2312 7 0.46899523848383495 2312 8 0.5132582393002778 2312 9 0.8884692082009593 2312 10-11 0.4062702929343729 2312 12-13 0.23740868387066172 2312 14-15 0.6159851835496042 2312 16-17 0.870079776166051 2312 18-19 0.6243089470888137 2312 20-21 0.9375774148887075 2312 22-23 0.7937938852710786 2312 24-25 0.48474111676326004 2312 26-27 0.9077560056999303 2312 28-29 0.9479081854952653 2312 30-31 0.8073433648481156 2312 32-33 0.9998108628346003 2312 34-35 1.3710584522766567 2312 36-37 1.7562668436940285 2312 38-39 1.5881866922292218 2312 40-41 1.7850589242531214 2312 42-43 2.2652744589469904 2312 44-45 1.3486991464663305 2312 46-47 1.5676393676660254 2312 48-49 2.1258345909727225 2312 50-51 2.3335847295444196 2312 52-53 2.651750911074352 2312 54-55 1.9868480693595174 2312 56-57 2.584137965073822 2312 58-59 1.3841774052629106 2312 60-61 1.1807969251668808 2312 62-63 1.3604563752861303 2312 64-65 2.058661846225217 2312 66-67 2.066078410756834 2312 68-69 1.8028149778704226 2312 70-71 1.9168895340226513 2312 72-73 1.600370082364357 2312 74-75 1.787718315510471 2312 76-77 1.8685678169963218 2312 78-79 2.026983005491587 2312 80-81 1.829587469083851 2312 82-83 1.3633219302816215 2312 84-85 1.0681449973783224 2312 86-87 1.3031716714675134 2312 88-89 1.195514753141083 2312 90-91 1.3797571249356047 2312 92-93 1.5234637354206413 2312 94-95 1.5273692510577668 2312 96-97 1.9920780204835147 2312 98-99 1.899707608220151 2312 100-101 2.727531811368099 2313 1 -0.4337936101897881 2313 2 -0.16591071609842345 2313 3 -0.2925029922137483 2313 4 -0.6054443467381816 2313 5 -0.21895860054981853 2313 6 -0.07689138605091728 2313 7 -0.20520438486833115 2313 8 -0.04543604820756997 2313 9 -1.184977119482653 2313 10-11 -0.8065357334309766 2313 12-13 -0.8533006695505563 2313 14-15 -0.456990334341171 2313 16-17 -0.3064738961503295 2313 18-19 -0.6652390755100654 2313 20-21 -0.8408281092795278 2313 22-23 -0.8794641184953988 2313 24-25 -1.4852839930923594 2313 26-27 -1.282137277413696 2313 28-29 -0.9660905590119597 2313 30-31 -1.5523553168844657 2313 32-33 -1.6849035125577458 2313 34-35 -1.7976483901589972 2313 36-37 -1.3341286365319576 2313 38-39 -1.4568541112861553 2313 40-41 -2.458506675244678 2313 42-43 -2.840500807845231 2313 44-45 -2.344803678392431 2313 46-47 -1.9795985482159537 2313 48-49 -1.541616758682018 2313 50-51 -2.3566224267644316 2313 52-53 -2.9852547386431674 2313 54-55 -2.1617708885814793 2313 56-57 -1.6680591472927802 2313 58-59 -1.491214936231131 2313 60-61 -1.201186753426967 2313 62-63 -0.8432473284175792 2313 64-65 -1.2294737469951222 2313 66-67 -1.7151519721684636 2313 68-69 -1.8106501822049061 2313 70-71 -1.5187664609553764 2313 72-73 -2.4619337468886258 2313 74-75 -1.9652634798442037 2313 76-77 -1.3079858553200658 2313 78-79 -2.338522330352731 2313 80-81 -2.3697220098866474 2313 82-83 -2.0972869837171224 2313 84-85 -1.7543597107196085 2313 86-87 -1.099370701413843 2313 88-89 -1.3049560566517613 2313 90-91 -1.1542981167467588 2313 92-93 -1.1821232074544383 2313 94-95 -0.7623357081387176 2313 96-97 -0.7591774722974058 2313 98-99 -0.6833118519179529 2313 100-101 -0.10485990237954823 2314 1 -0.007057688921335625 2314 2 -0.24354876967961303 2314 3 0.1500885878846674 2314 4 0.4820351502547098 2314 5 -0.11562344472696395 2314 6 -0.07935174690384628 2314 7 0.23602032808956608 2314 8 -0.0470762887761893 2314 9 -0.29432649072376904 2314 10-11 0.15806240762970702 2314 12-13 -0.003929428435192506 2314 14-15 0.271003104696554 2314 16-17 0.01255289444562635 2314 18-19 -0.018164171190761635 2314 20-21 0.3491664232519085 2314 22-23 0.2767687956653475 2314 24-25 0.3739286914347062 2314 26-27 0.7879830068946774 2314 28-29 0.3998197745036265 2314 30-31 0.12902795266173683 2314 32-33 0.21695542675335133 2314 34-35 0.8641886792778521 2314 36-37 -0.43459337135973186 2314 38-39 -0.16748833763935522 2314 40-41 -0.047079665949986804 2314 42-43 0.028416633379485745 2314 44-45 0.07181742603621899 2314 46-47 0.21668357316184483 2314 48-49 0.4660376973048628 2314 50-51 0.0343015754225533 2314 52-53 0.4850842444076804 2314 54-55 0.9202411398493595 2314 56-57 0.2872442972124105 2314 58-59 0.673902614342957 2314 60-61 1.4588733887272198 2314 62-63 0.7938494457759724 2314 64-65 1.6312424913865122 2314 66-67 1.1765921503028345 2314 68-69 1.0429583470460528 2314 70-71 1.474535890056103 2314 72-73 1.5038945745985295 2314 74-75 1.0622105496801275 2314 76-77 0.7852344836629861 2314 78-79 1.0640200425286253 2314 80-81 1.7116065849739321 2314 82-83 1.0813625515480538 2314 84-85 1.1577507321453488 2314 86-87 1.7628490908223533 2314 88-89 0.9383462943597181 2314 90-91 1.0117762408256858 2314 92-93 0.4685055514301979 2314 94-95 0.12981847447473172 2314 96-97 0.6127470766364418 2314 98-99 0.1491102366550372 2314 100-101 0.8601482988232974 2315 1 -0.12847345953065314 2315 2 0.17401395433472544 2315 3 -0.21505478129096645 2315 4 0.20246525778159707 2315 5 0.3055235086780499 2315 6 0.16392782298862585 2315 7 0.3301063495949421 2315 8 0.12899898004101829 2315 9 0.24958031931207358 2315 10-11 0.2534925151565872 2315 12-13 -0.09129501983303356 2315 14-15 0.5743185168829328 2315 16-17 -0.004920223833948967 2315 18-19 -0.18819105291118632 2315 20-21 -0.26844110362980445 2315 22-23 -0.0950950036178071 2315 24-25 0.5078892649114053 2315 26-27 0.6461819316258541 2315 28-29 0.6238341114211892 2315 30-31 0.8003989204036728 2315 32-33 0.14101456653829558 2315 34-35 -0.10579339957519807 2315 36-37 0.7215446214718071 2315 38-39 0.5789274329699623 2315 40-41 0.4609848501790452 2315 42-43 0.39490408857661663 2315 44-45 0.9875880353552162 2315 46-47 0.13245418248084206 2315 48-49 -0.05703577939764415 2315 50-51 -0.3678041593444661 2315 52-53 -0.10519353337009818 2315 54-55 -0.05713341212197065 2315 56-57 0.047100928036783785 2315 58-59 0.06704777563327724 2315 60-61 0.6206117399816975 2315 62-63 0.3789748938046422 2315 64-65 1.0100507351141026 2315 66-67 1.0961710033494292 2315 68-69 0.906981644537094 2315 70-71 1.1344821266152465 2315 72-73 0.949907119401395 2315 74-75 0.9844775747697305 2315 76-77 1.0005122614407647 2315 78-79 1.043186709195293 2315 80-81 1.088846728343107 2315 82-83 1.0670256339853275 2315 84-85 0.9848116640449867 2315 86-87 0.6990050048008492 2315 88-89 0.6538480864744152 2315 90-91 0.3404052730837499 2315 92-93 -0.1385733016163968 2315 94-95 -0.505038156349638 2315 96-97 -0.3574590165535234 2315 98-99 0.47841131192385333 2315 100-101 0.8178095891458774 2316 1 -0.3312512373084324 2316 2 -0.4070971567763877 2316 3 -0.2689436701798513 2316 4 0.01913192444825995 2316 5 -0.26336538021083555 2316 6 -0.057738843678038165 2316 7 0.003439682928281229 2316 8 0.07066564670768116 2316 9 0.08180254153429445 2316 10-11 -0.2848408181767397 2316 12-13 -0.5229616864997055 2316 14-15 -0.5001259275615055 2316 16-17 -0.6632535571672804 2316 18-19 -0.47735771957786 2316 20-21 0.337392229703525 2316 22-23 0.4943494408266318 2316 24-25 0.49344482046696214 2316 26-27 -0.292984735040811 2316 28-29 0.20772300031007518 2316 30-31 0.06789892040367107 2316 32-33 -0.08481876679503131 2316 34-35 0.06031771153591592 2316 36-37 0.24460017702736536 2316 38-39 -0.018294789252259136 2316 40-41 -0.2190151498209545 2316 42-43 -0.09787368920116535 2316 44-45 -0.30963418686700805 2316 46-47 -0.29476803974137766 2316 48-49 -0.3350913349531979 2316 50-51 -0.08585971490002464 2316 52-53 -0.14991575559231762 2316 54-55 0.01814436565580735 2316 56-57 0.41821203914789606 2316 58-59 1.029547775633283 2316 60-61 1.2883895177594766 2316 62-63 1.0667526715824174 2316 64-65 0.8786618462252171 2316 66-67 0.9325598922383129 2316 68-69 1.6689260889815394 2316 70-71 1.4711487932819125 2316 72-73 1.4801848971791713 2316 74-75 0.8525331303252877 2316 76-77 1.1352344836629875 2316 78-79 1.399020042528626 2316 80-81 0.5227356172319979 2316 82-83 0.8831367450964365 2316 84-85 1.367589441822762 2316 86-87 1.573171671467513 2316 88-89 0.9849591975855247 2316 90-91 1.0129052730837493 2316 92-93 1.3075378094947148 2316 94-95 0.94385073253925 2316 96-97 1.3722632056686983 2316 98-99 0.7150779785905215 2316 100-101 1.515309589145879 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1.0 3 0.0 4 1.0 5 3.0 6 6.0 7 16.0 8 23.0 9 93.0 10 109.0 11 234.0 12 339.0 13 540.0 14 702.0 15 1021.0 16 1458.0 17 1989.0 18 1783.0 19 1150.0 20 817.0 21 872.0 22 803.0 23 912.0 24 899.0 25 1001.0 26 1040.0 27 1158.0 28 1321.0 29 1500.0 30 1733.0 31 2233.0 32 2732.0 33 3692.0 34 5277.0 35 7967.0 36 12553.0 37 19766.0 38 17276.0 39 1634.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.543446322309244 20.554692371922926 15.391444969401022 19.510416336366806 2 15.64329029940626 22.545270141779532 40.10607053056395 21.70536902825026 3 19.89561983645699 27.270902444693302 30.81222135356139 22.021256365288313 4 12.287911762841507 18.389080229044733 37.596931983857 31.726076024256766 5 12.137091662088492 39.822719012804804 33.3463635211089 14.693825803997804 6 29.408160246793585 38.29949077693494 16.970228410843706 15.322120565427769 7 26.28943309316035 37.11834682105352 19.679041561899126 16.913178523886998 8 26.58630380121284 34.74443763602172 19.110655651108246 19.558602911657193 9 27.255055253871994 17.64743169860756 20.473514061740655 34.623998985779785 10-11 24.744331988082912 28.752614786485513 25.9444925729499 20.55856065248167 12-13 26.452751246724702 26.707907192967628 27.312251711605107 19.527089848702563 14-15 22.683668941618947 27.38869989646502 27.703530754114986 22.224100407801046 16-17 19.965875715764785 30.870327719906182 28.169438164261418 20.994358400067615 18-19 19.43552306294504 28.30889344348892 33.03980814334312 19.215775350222916 20-21 21.06197308090519 26.937583197751813 33.16341623174932 18.837027489593677 22-23 20.537445259715906 27.276377025192417 33.049661125285915 19.136516589805762 24-25 20.473514061740655 27.592600418365837 33.22046611870602 18.713419401187483 26-27 19.85948601463247 27.68019862127255 33.804706690261746 18.655608673833232 28-29 19.201390356252375 28.59263407006719 33.55185310400203 18.6541224696784 30-31 19.544340439918017 27.810763412005834 33.13172185010671 19.513174297969446 32-33 19.575506581866588 28.317345278593614 32.675850994147105 19.431297145392694 34-35 20.00021129587762 28.07171382086335 32.97536290066981 18.95271198258922 36-37 20.367337883237898 27.842986033342488 33.0371669448729 18.752509138546706 38-39 19.700171149660868 28.437255689141505 32.796817884083076 19.065755277114544 40-41 19.281378924181354 28.873734198946682 32.50064709704343 19.34423977982853 42-43 19.818658325231432 29.010440884304856 31.725810542334195 19.445090248129517 44-45 20.059374141610498 28.73888055444038 31.47833160775033 19.72341369619879 46-47 20.37262028017833 29.7356688571006 30.572928772159656 19.318782090561413 48-49 20.78042132397997 29.472077249772855 30.206330424493416 19.541171001753757 50-51 20.3916369091639 29.7805692310943 30.219008177150464 19.608785682591332 52-53 20.27436769708623 30.539121431740867 29.2058444439749 19.980666427198006 54-55 19.86551017389651 30.373254167811186 29.294588712574214 20.46664694571809 56-57 20.476683499904915 29.68971200371881 29.817546009677354 20.016058486698924 58-59 20.70646776681387 29.26078137215543 29.6617152999345 20.371035561096203 60-61 19.829589874701544 29.566103915312613 30.597227798085658 20.007078411900185 62-63 19.643121262704163 29.12660848986836 30.725061804044202 20.505208443383268 64-65 19.33145984321846 28.9755319373719 30.960656707587635 20.732351511822003 66-67 19.400659243138165 28.92323620766159 30.179918439791244 21.496186109409006 68-69 19.576034821560633 28.907917256534326 29.712954550256725 21.80309337164832 70-71 19.80370612969341 29.14562511885393 29.6944661609652 21.35620259048746 72-73 19.373719018741944 28.344285502989834 30.423436938745326 21.858558539522893 74-75 18.94373190779048 29.012508715954954 29.533881293975956 22.509878082278615 76-77 18.89513385593847 28.028926405645826 30.160901810805672 22.915037927610033 78-79 19.33991167832316 28.411900183827417 29.45887125742177 22.789316880427663 80-81 18.766771610285886 28.323684154922134 29.195279650094026 23.714264584697954 82-83 18.42456961443581 27.955120518522826 29.556223488830312 24.064086378211055 84-85 18.059458659961543 27.797557419654744 29.850296870708053 24.29268704967566 86-87 18.203139856741394 27.78065374944535 28.904747818370062 25.11145857544319 88-89 17.455680689669745 27.895810002746845 28.911086694698586 25.737422612884824 90-91 17.773293041144523 27.47458844910294 29.342680839373642 25.409437670378903 92-93 17.68823196919192 27.865145984437483 28.94860565976936 25.498016386601236 94-95 17.048407885562153 28.591501679802228 27.974517717159337 26.38557271747628 96-97 17.222198744902485 29.51063874743804 27.540832928349566 25.72632957930991 98-99 17.61962114248888 29.695308125468816 26.517384551995182 26.167686180047117 100-101 17.118825847831484 28.40757197123792 26.213961548418457 28.259640632512138 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 202.0 1 128.5 2 63.5 3 72.0 4 72.0 5 76.0 6 102.5 7 191.0 8 293.0 9 351.0 10 344.5 11 320.5 12 348.0 13 366.0 14 379.0 15 400.5 16 402.0 17 407.0 18 431.0 19 449.0 20 433.0 21 481.5 22 545.0 23 605.5 24 694.0 25 797.0 26 918.0 27 1058.0 28 1196.0 29 1332.5 30 1535.5 31 1711.0 32 1829.5 33 1980.5 34 2125.5 35 2237.0 36 2471.5 37 2625.0 38 2643.5 39 2714.5 40 2785.5 41 2900.5 42 3056.0 43 3191.5 44 3205.0 45 3204.0 46 3223.0 47 3449.0 48 3921.5 49 3760.5 50 3240.5 51 2951.0 52 2795.5 53 2682.5 54 2413.0 55 2140.5 56 1914.0 57 1686.5 58 1513.0 59 1319.0 60 1038.5 61 794.5 62 617.5 63 469.5 64 326.0 65 198.5 66 142.0 67 134.5 68 104.5 69 64.0 70 45.0 71 30.0 72 21.5 73 17.5 74 12.5 75 12.5 76 13.0 77 8.5 78 5.0 79 3.5 80 3.0 81 3.0 82 2.5 83 1.0 84 1.0 85 1.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.04542861368774695 2 0.0 3 0.0 4 0.0 5 0.002112958776174277 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.00633887632852283 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.002641198470217846 24-25 0.0 26-27 0.0015847190821307075 28-29 0.002112958776174277 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 5.282396940435693E-4 42-43 0.027468464090265602 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 5.282396940435693E-4 84-85 0.0 86-87 0.0 88-89 0.0 90-91 0.01267775265704566 92-93 0.0036976778583049843 94-95 0.0 96-97 0.0 98-99 0.0010564793880871385 100-101 0.016375430515350644 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 94654.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 74.19549094597164 #Duplication Level Percentage of deduplicated Percentage of total 1 89.08285750900625 66.09546347750755 2 6.648250722635948 9.865404525957699 3 1.8425436785373566 4.101252984554272 4 0.7916957382277977 2.349610159105796 5 0.4399891782596933 1.6322606545946288 6 0.28193481325378406 1.2550975130475204 7 0.19792393455694943 1.0279544446087858 8 0.15520653860940636 0.9212500264119847 9 0.06407609392131457 0.42787415217529107 >10 0.4499565706474533 5.879307794704925 >50 0.028478263965028693 1.457941555560251 >100 0.011391305586011476 1.6798022270585502 >500 0.004271739594754304 2.2407927821328206 >1k 0.0014239131982514345 1.0659877025799227 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1009 1.0659877025799227 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 888 0.9381536966213789 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 645 0.6814292053162043 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 588 0.6212098801952374 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 465 0.4912629154605194 No Hit GTCCCAGGGTGTGCTTGTCAAAGAGATATTCTGCCATGCCAGCTTCAGGG 226 0.23876434170769328 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 216 0.22819954782682192 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 189 0.19967460434846918 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 162 0.17114966087011643 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 121 0.12783400595854374 No Hit CTACTGGAACTGCACAAACTGGCTACTGACAAGAATGATCCCCACTTATG 107 0.11304329452532381 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 104 0.10987385636106241 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.0010564793880871385 0.0 24-25 0.0 0.0 0.0 0.002112958776174277 0.0 26-27 0.0 0.0 0.0 0.0036976778583049847 0.0 28-29 0.0 0.0 0.0 0.007923595410653538 0.0 30-31 0.0 0.0 0.0 0.021129587761742768 0.0 32-33 0.0 0.0 0.0 0.038033257971136984 0.0 34-35 0.0 0.0 0.0 0.04859805185200837 0.0 36-37 0.0 0.0 0.0 0.06233228389714117 0.0 38-39 0.0 0.0 0.0 0.09402666553975532 0.0 40-41 0.0 0.0 0.0 0.1785450165867264 0.0 42-43 0.0 0.0 0.0 0.3121896591797494 0.0 44-45 0.0 0.0 0.0 0.4357977475859446 0.0 46-47 0.0 0.0 0.0 0.7506286052359119 0.0 48-49 0.0 0.0 0.0 1.0776089758488812 0.0 50-51 0.0 0.0 0.0 1.2910178122424831 0.0 52-53 0.0 0.0 0.0 1.575739007331967 0.0 54-55 0.0 0.0 0.0 1.8604602024214507 0.0 56-57 0.0 0.0 0.0 2.533437572632958 0.0 58-59 0.0 0.0 0.0 3.266634267965432 0.0 60-61 0.0 0.0 0.0 4.020432311365605 0.0 62-63 0.0 0.0 0.0 4.937984659919286 0.0 64-65 0.0 0.0 0.0 5.884061951951318 0.0 66-67 0.0 0.0 0.0 6.948993175143153 0.0 68-69 0.0 0.0 0.0 8.07308724406787 0.0 70-71 0.0 0.0 0.0 9.18344708094745 0.0 72-73 0.0 0.0 0.0 10.294335157521076 0.0 74-75 0.0 0.0 0.0 11.412090350117268 0.0 76-77 0.0 0.0 0.0 12.892746212521395 0.0 78-79 0.0 0.0 0.0 14.079700805037294 0.0 80-81 0.0 0.0 0.0 15.61846303378621 0.0 82-83 0.0 0.0 0.0 17.424514547721174 0.0 84-85 0.0 0.0 0.0 19.518456694909883 0.0 86-87 0.0 0.0 0.0 21.307604538635452 0.0 88-89 0.0 0.0 0.0 23.15274578992964 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGTAGCT 15 6.587489E-4 95.041756 8 TCGTAGC 20 0.0020645782 71.28132 7 TTCGTAG 25 0.005008852 56.99493 6 TATTCGT 25 0.005008852 56.99493 4 ATATTCG 25 0.005008852 56.99493 3 GTCCCAG 80 1.0639915E-5 35.64066 1 TGGGGAG 55 0.0025621983 34.56064 8 TACACTG 55 0.002568853 34.54238 5 GGTGTGC 85 1.6116343E-5 33.544147 8 GTGTGCT 90 2.3826384E-5 31.680584 9 GAATGCG 30 0.0039444105 31.663847 82-83 CTATTGT 60 0.0039345333 31.663847 4 GGTATCA 1165 0.0 29.369125 1 GGGAATC 65 0.0058048773 29.243616 9 ACTGTGC 65 0.0058048773 29.243616 8 GAACTGC 100 4.8911952E-5 28.512526 7 GACAGCT 70 0.008335492 27.154789 7 TAGAACA 70 0.008357027 27.140444 4 TCTTATC 35 0.008377818 27.140444 94-95 TACCTGG 70 0.008357027 27.140444 2 >>END_MODULE