FastQCFastQC Report
Thu 26 May 2016
SRR937692_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937692_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences60205
Sequences flagged as poor quality0
Sequence length101
%GC40

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9831.6327547545884895No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7191.1942529690225065No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5310.8819865459679428No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1980.32887633917448716No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1680.2790465908147164No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1650.27406361597873935No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT1470.24416576696287684No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT1100.18270907731915953No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1060.17606511087119012No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT1000.16609916119923596No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1000.16609916119923596No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC820.13620131218337347No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT770.12789635412341166No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN750.12457437089942697No Hit
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT640.106303463167511No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT630.10464247155551865No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGTCATA250.004997692557.00157
CCTACCC250.004997692557.00153
GTATGTC250.004997692557.00151
CTACCCT250.004997692557.00154
GGTATCA3350.055.2999571
ATACAGT352.7663258E-454.2871366
GTATCAA6100.041.271581
ACAGTCT608.1960054E-539.584378
ACTGGTC651.313061E-436.539428
TCAACGC7500.032.9341964
ATCAACG7550.032.716093
TATCAAC7650.032.288432
CAACGCA7700.032.0787665
GCACTGG600.003920983531.6674966
AACGCAG7950.031.0700026
CGGTGGT404.4693163E-429.68827894-95
CATGGGG650.005799939429.2315374
GCAGAGT8750.028.2293139
ACGCAGA8800.027.5291337
CGCAGAG8850.027.37368
Produced by FastQC (version 0.11.5)