##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937692_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 60205 Sequences flagged as poor quality 0 Sequence length 101 %GC 40 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.022506436342496 33.0 31.0 34.0 31.0 34.0 2 32.0964537829084 33.0 31.0 34.0 31.0 34.0 3 32.11278133045428 34.0 31.0 34.0 30.0 34.0 4 35.696819201063036 37.0 35.0 37.0 35.0 37.0 5 35.72628519225978 37.0 35.0 37.0 35.0 37.0 6 35.80745785233785 37.0 36.0 37.0 35.0 37.0 7 35.75830911053899 37.0 36.0 37.0 35.0 37.0 8 35.7784901586247 37.0 36.0 37.0 35.0 37.0 9 37.34480524873349 39.0 37.0 39.0 35.0 39.0 10-11 37.31285607507682 39.0 37.0 39.0 35.0 39.0 12-13 37.24034548625529 39.0 37.0 39.0 35.0 39.0 14-15 38.33796196329209 40.0 38.0 41.0 34.0 41.0 16-17 38.31789718461921 40.0 38.0 41.0 34.0 41.0 18-19 38.371904326883154 40.0 38.0 41.0 34.0 41.0 20-21 38.40695955485425 40.0 38.0 41.0 34.0 41.0 22-23 38.36378207790051 40.0 38.0 41.0 34.0 41.0 24-25 38.266871522298814 40.0 38.0 41.0 34.0 41.0 26-27 38.15032804584337 40.0 38.0 41.0 34.0 41.0 28-29 37.95654845943028 40.0 38.0 41.0 34.0 41.0 30-31 37.57212856075077 40.0 37.0 41.0 33.0 41.0 32-33 37.240694294493814 40.0 36.0 41.0 33.0 41.0 34-35 37.1050411095424 40.0 35.0 41.0 33.0 41.0 36-37 36.87396395648202 40.0 35.0 41.0 32.5 41.0 38-39 36.643891703346895 39.5 35.0 41.0 31.5 41.0 40-41 36.30976663067851 39.0 35.0 41.0 31.0 41.0 42-43 35.864861722448296 39.0 35.0 41.0 30.0 41.0 44-45 35.28912880989951 39.0 35.0 41.0 26.5 41.0 46-47 34.56103313678266 39.0 35.0 41.0 20.5 41.0 48-49 33.92351964122581 39.0 35.0 40.0 8.0 41.0 50-51 32.91574620048169 37.5 33.5 39.5 2.0 40.5 52-53 32.92218254297816 38.0 33.5 39.5 2.0 40.5 54-55 33.10308113944025 38.0 34.0 40.0 2.0 41.0 56-57 32.78621376962046 38.0 33.5 40.0 2.0 41.0 58-59 32.48833153392576 38.0 33.0 40.0 2.0 41.0 60-61 32.08531683414999 38.0 33.0 40.0 2.0 41.0 62-63 31.714309442737314 37.0 32.0 40.0 2.0 41.0 64-65 31.270808072419236 36.5 31.5 40.0 2.0 41.0 66-67 30.69530769869612 36.0 31.0 39.0 2.0 41.0 68-69 30.21555518644631 35.5 30.0 39.0 2.0 40.5 70-71 29.760269080641145 35.0 29.0 39.0 2.0 40.0 72-73 29.33027987708662 35.0 29.0 38.0 2.0 40.0 74-75 28.826501121169336 35.0 28.5 37.0 2.0 39.5 76-77 28.31783074495474 34.5 26.5 37.0 2.0 39.0 78-79 27.834424051158543 34.0 26.0 36.0 2.0 39.0 80-81 27.37785067685408 34.0 25.5 36.0 2.0 38.5 82-83 26.921609500872023 34.0 24.5 35.0 2.0 37.0 84-85 26.446657254380867 34.0 23.0 35.0 2.0 37.0 86-87 25.90607922929989 33.5 20.0 35.0 2.0 36.0 88-89 25.419275807657172 33.0 18.0 35.0 2.0 36.0 90-91 24.88925338427041 32.5 9.5 35.0 2.0 35.5 92-93 24.278847271821277 32.0 2.0 35.0 2.0 35.0 94-95 23.576928826509423 31.5 2.0 34.0 2.0 35.0 96-97 22.796553442405116 31.0 2.0 34.0 2.0 35.0 98-99 21.747504360102983 30.5 2.0 34.0 2.0 35.0 100-101 19.020023253882567 26.0 2.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.9793236242768053 1101 2 0.5944600944217449 1101 3 0.4058820291829832 1101 4 0.742192025533619 1101 5 0.641972318052531 1101 6 0.6738101560234 1101 7 0.6051671145200004 1101 8 0.7330173855855335 1101 9 0.9171024636063692 1101 10-11 0.828771529083383 1101 12-13 0.684177243754533 1101 14-15 0.6050752745837045 1101 16-17 0.4506874984750624 1101 18-19 0.9614230048786041 1101 20-21 1.0850475069838552 1101 22-23 0.40605253088659055 1101 24-25 -0.1834105313868477 1101 26-27 0.24079172752279732 1101 28-29 0.3636284710805242 1101 30-31 0.5443636079069378 1101 32-33 0.6862693728059028 1101 34-35 0.7781623291088877 1101 36-37 1.1177469047954318 1101 38-39 1.3627605293450387 1101 40-41 1.1824651145750735 1101 42-43 1.434719648545176 1101 44-45 1.611114019564262 1101 46-47 1.6671084766248043 1101 48-49 1.3300357903503084 1101 50-51 2.103538127467214 1101 52-53 2.2605050377260447 1101 54-55 1.9825430451883221 1101 56-57 2.6540872250896292 1101 58-59 2.247987654770057 1101 60-61 2.42586455942887 1101 62-63 2.192058634364951 1101 64-65 2.120355869037038 1101 66-67 2.2984372638228905 1101 68-69 2.9717548926879154 1101 70-71 3.641679523117606 1101 72-73 3.2247437131988335 1101 74-75 3.612739682179452 1101 76-77 3.650298003516987 1101 78-79 3.72701732689384 1101 80-81 3.928397468216769 1101 82-83 3.376511860530826 1101 84-85 3.971421699786749 1101 86-87 4.1717830269721965 1101 88-89 4.600749386862834 1101 90-91 5.060048017428059 1101 92-93 5.140930412735241 1101 94-95 5.439771466185473 1101 96-97 4.821976511962092 1101 98-99 5.3069406346609505 1101 100-101 4.498960011127551 1102 1 -0.5072564623032818 1102 2 -0.19515029518864324 1102 3 -0.11987554657459043 1102 4 -0.5917906584490709 1102 5 -0.6311878551076404 1102 6 -0.4638521816389414 1102 7 -0.27362076426788207 1102 8 -0.6591904066222583 1102 9 -0.8865771900732824 1102 10-11 -0.373068297756447 1102 12-13 -0.24071453113724317 1102 14-15 -0.8138641193556921 1102 16-17 -0.7250700772825098 1102 18-19 -0.7284038349482387 1102 20-21 -0.15640270946636292 1102 22-23 -0.10195612712206525 1102 24-25 0.35197907900276704 1102 26-27 -0.25336411663304403 1102 28-29 -0.42305984060779167 1102 30-31 0.7997748633181985 1102 32-33 0.7997975113340416 1102 34-35 -0.08828788734133042 1102 36-37 -0.2844175973690781 1102 38-39 0.09912416570867322 1102 40-41 -0.21288120577124658 1102 42-43 0.19218718101271293 1102 44-45 -0.036180352730106335 1102 46-47 1.1108314203477434 1102 48-49 1.2056851409996554 1102 50-51 0.2937978677269548 1102 52-53 -0.4406854384644312 1102 54-55 -0.03347427082898946 1102 56-57 0.7441305151329196 1102 58-59 0.3549140616964621 1102 60-61 0.01471737328168743 1102 62-63 0.04876859107491072 1102 64-65 -0.30659218291101453 1102 66-67 -0.8262380608524325 1102 68-69 -1.6954528995198785 1102 70-71 -1.4533421219040399 1102 72-73 -0.534780096324976 1102 74-75 -0.2558750364352669 1102 76-77 -0.4358491826302 1102 78-79 -0.13683548695897585 1102 80-81 -0.08826919844990044 1102 82-83 -0.5943755853566195 1102 84-85 -0.7695956162305642 1102 86-87 -1.0560308258416562 1102 88-89 -1.4581250720116223 1102 90-91 -0.8973112899312454 1102 92-93 -1.9030089812041489 1102 94-95 -2.076786975372972 1102 96-97 -2.3562702412846583 1102 98-99 -1.70236672464641 1102 100-101 -1.5262564390888969 1103 1 -0.21432716937398766 1103 2 0.42303152299317404 1103 3 0.5003264736274318 1103 4 0.06679520013678797 1103 5 -0.4231070470268321 1103 6 -0.19920571699247347 1103 7 -0.5281662188133325 1103 8 -0.20666515409700992 1103 9 0.7639278604317639 1103 10-11 0.3077397830516375 1103 12-13 0.7976693072465935 1103 14-15 1.0911863856948116 1103 16-17 0.31735416514172954 1103 18-19 0.24634363979923535 1103 20-21 0.25369830063465315 1103 22-23 0.44652872136278177 1103 24-25 -0.37428354725985713 1103 26-27 -0.40285906612799494 1103 28-29 -0.35740327495122415 1103 30-31 0.00785567139900678 1103 32-33 0.27555508709162524 1103 34-35 0.3329242338707914 1103 36-37 0.23679452384305222 1103 38-39 0.9294271960117015 1103 40-41 0.8042905114004668 1103 42-43 1.3406720294975543 1103 44-45 2.4789711624214092 1103 46-47 1.5512354607517835 1103 48-49 1.7248770601915808 1103 50-51 1.8948079687370587 1103 52-53 2.3370923393133474 1103 54-55 1.6301620928073746 1103 56-57 1.3905951615975631 1103 58-59 0.8003686071510074 1103 60-61 1.2389597975241067 1103 62-63 1.6507887930951135 1103 64-65 0.7580542817354505 1103 66-67 1.633357898743526 1103 68-69 1.868183464116484 1103 70-71 1.3567588881969712 1103 72-73 -0.10144676299164246 1103 74-75 -0.4134507940110268 1103 76-77 -0.3116067583877751 1103 78-79 -0.038855688979175795 1103 80-81 -0.24382475400545545 1103 82-83 -0.18023417121520424 1103 84-85 0.7162629696280227 1103 86-87 0.7793227095118844 1103 88-89 1.5499557360691831 1103 90-91 1.1370321444121885 1103 92-93 0.34648596829079636 1103 94-95 1.0838190852330918 1103 96-97 1.203325718311298 1103 98-99 -0.025599047878731795 1103 100-101 2.4303092174767578 1104 1 0.1459902909434696 1104 2 0.09446009442174486 1104 3 0.7987391720401291 1104 4 0.6588586922002833 1104 5 0.45149612757634117 1104 6 -0.0642850820718408 1104 7 -0.08530907595618942 1104 8 0.661588814156957 1104 9 0.09567389217779976 1104 10-11 0.5252001005119524 1104 12-13 0.6722724818497667 1104 14-15 0.8729324174408433 1104 16-17 0.022116069903638902 1104 18-19 0.401899195354801 1104 20-21 -0.039952493016144786 1104 22-23 -0.02251889768483295 1104 24-25 -0.5107914837677967 1104 26-27 0.0026964894275565143 1104 28-29 0.0957713282233783 1104 30-31 0.5324588460021786 1104 32-33 -0.010159198622666565 1104 34-35 0.742448043394603 1104 36-37 -0.19177690472837838 1104 38-39 -0.23247756589305624 1104 40-41 0.14079844790840212 1104 42-43 1.4406720294975628 1104 44-45 1.3789711624214007 1104 46-47 1.7147275242438482 1104 48-49 2.478845314159834 1104 50-51 3.008300032229119 1104 52-53 2.8736002758212855 1104 54-55 2.8873049499502343 1104 56-57 2.7017062727086802 1104 58-59 2.759892416674816 1104 60-61 2.2651502737145854 1104 62-63 2.834915777222097 1104 64-65 2.8405939642751328 1104 66-67 2.5127229781086022 1104 68-69 2.0134215593545797 1104 70-71 2.391679523117606 1104 72-73 2.4747437131988335 1104 74-75 2.3984539678937367 1104 76-77 2.316964670183655 1104 78-79 2.28654113641765 1104 80-81 1.4641117539310535 1104 82-83 1.501511860530826 1104 84-85 1.2630883664534203 1104 86-87 2.3682115984007694 1104 88-89 1.9578922440056914 1104 90-91 1.6910003983804458 1104 92-93 1.676644698449529 1104 94-95 0.7790571804711881 1104 96-97 -0.10064253565695225 1104 98-99 -0.06210698438666995 1104 100-101 -0.2927066555391171 1105 1 0.040078960894209814 1105 2 0.2988935919586915 1105 3 -0.42457774093195866 1105 4 -0.3140477117406064 1105 5 -0.9434135604367953 1105 6 0.6245490722795566 1105 7 0.6511441260142519 1105 8 0.6492735432209997 1105 9 0.09649491024019596 1105 10-11 -0.2884287993238388 1105 12-13 0.12793750648030766 1105 14-15 -0.18659139208296693 1105 16-17 0.1288484180152878 1105 18-19 -0.28200885062221914 1105 20-21 -1.0087538066450463 1105 22-23 -0.5097931177176775 1105 24-25 -0.5953563441947267 1105 26-27 -1.1323609818368041 1105 28-29 -0.5926523170968139 1105 30-31 -0.3538301523557905 1105 32-33 -0.615249510609523 1105 34-35 -0.5785700190027683 1105 36-37 -1.1195273152374128 1105 38-39 -1.0165498154825485 1105 40-41 -1.153947036492248 1105 42-43 -2.2949601544104894 1105 44-45 -3.0922932053946894 1105 46-47 -3.9158143476773333 1105 48-49 -4.468609529846734 1105 50-51 -3.8435062075081596 1105 52-53 -4.547581990188569 1105 54-55 -3.3663896313305557 1105 56-57 -3.964960393957991 1105 58-59 -4.265148634228304 1105 60-61 -4.5012700875333636 1105 62-63 -4.794805076636688 1105 64-65 -5.621639204854592 1105 66-67 -4.327178499723907 1105 68-69 -4.107678604849031 1105 70-71 -4.143624253565481 1105 72-73 -4.748573199773254 1105 74-75 -4.429542748034013 1105 76-77 -4.738043539996973 1105 78-79 -3.927744577868065 1105 80-81 -3.833096784656796 1105 82-83 -3.954563673130913 1105 84-85 -3.5542351146631646 1105 86-87 -2.947880355622221 1105 88-89 -1.8048335359614676 1105 90-91 -2.7104774341318745 1105 92-93 -2.1033224608279752 1105 94-95 -3.1667556274105877 1105 96-97 -3.546865852569926 1105 98-99 -5.8650626494113 1105 100-101 -5.677353617772285 1106 1 -0.5654382804851004 1106 2 -0.7769684770068253 1106 3 -0.6907846374836808 1106 4 -0.8754270220854323 1106 5 -0.7875514914712767 1106 6 -1.3147612725480329 1106 7 -0.5281662188133325 1106 8 -0.7355540429858962 1106 9 -0.37384991734600703 1106 10-11 -0.37670466139280734 1106 12-13 -0.5534418038645157 1106 14-15 -0.8865913920829698 1106 16-17 -1.0759791681916013 1106 18-19 -0.40476747131187096 1106 20-21 -0.2218572549209057 1106 22-23 -0.5401379453038828 1106 24-25 -0.22983910281541853 1106 26-27 -0.7606368439057718 1106 28-29 -0.7685143860623427 1106 30-31 -0.5165887730454415 1106 32-33 -0.091111579575049 1106 34-35 0.20625756720412625 1106 36-37 -0.5898721428236158 1106 38-39 -1.077239470654959 1106 40-41 -1.380153933043978 1106 42-43 -1.1259946371691072 1106 44-45 -0.734362170911929 1106 46-47 -0.9109867614704399 1106 48-49 0.12932150463602454 1106 50-51 -1.8607475868185013 1106 52-53 -0.7606854384644315 1106 54-55 -0.3298379071926263 1106 56-57 -0.2449603939579923 1106 58-59 1.1603686071510069 1106 60-61 0.8456264641907758 1106 62-63 0.09967768198400151 1106 64-65 0.7824987261798917 1106 66-67 0.19558012096574728 1106 68-69 -0.2718165358835165 1106 70-71 -0.09879666735858095 1106 72-73 -0.3547800963249763 1106 74-75 0.4932158726556395 1106 76-77 0.7750599082788909 1106 78-79 -0.04774457786806252 1106 80-81 0.0717308015500997 1106 82-83 0.3619880510070175 1106 84-85 1.0740407474057996 1106 86-87 1.3548782650674376 1106 88-89 1.1455112916247394 1106 90-91 2.6681432555232973 1106 92-93 1.9642637460685783 1106 94-95 0.4104857518997598 1106 96-97 1.4455479405335225 1106 98-99 0.6921787298990445 1106 100-101 -1.1208018936343542 1107 1 0.0745617195149002 1107 2 0.19694456647143355 1107 3 0.20660666686414686 1107 4 0.7054425431319586 1107 5 0.8107093780939394 1107 6 0.7669778578867508 1107 7 0.619659868143188 1107 8 0.30444595701410293 1107 9 -0.9964586129981825 1107 10-11 -0.584530748349323 1107 12-13 0.12568863091809135 1107 14-15 0.16123469487354924 1107 16-17 0.8927164839823121 1107 18-19 0.45262383303595755 1107 20-21 0.6111862233399705 1107 22-23 0.7233403155656788 1107 24-25 0.5875522015324108 1107 26-27 0.7237109821811814 1107 28-29 0.4793117008941863 1107 30-31 0.5764547052154327 1107 32-33 -0.39111157957504616 1107 34-35 -0.8641772154045668 1107 36-37 -0.038567794997533156 1107 38-39 -2.680717731524524 1107 40-41 -1.7836321939135402 1107 42-43 -0.7912120284734527 1107 44-45 -0.5195795622162791 1107 46-47 -0.11446502233999922 1107 48-49 -0.9393741475378903 1107 50-51 -2.512921499861978 1107 52-53 -2.2537289167253007 1107 54-55 -2.1220118202361036 1107 56-57 -2.725829959175382 1107 58-59 -2.582240088501166 1107 60-61 -3.076982231461397 1107 62-63 -2.7385831875812165 1107 64-65 -1.8757621433853267 1107 66-67 -1.6052894442516425 1107 68-69 -2.418773057622648 1107 70-71 -3.298796667358584 1107 72-73 -3.291301835455414 1107 74-75 -2.8815667360400106 1107 76-77 -2.5101574830254556 1107 78-79 -2.6233967517811116 1107 80-81 -2.770877894102071 1107 82-83 -2.818881514210375 1107 84-85 -2.714654904768114 1107 86-87 -1.704252169715172 1107 88-89 -2.1901408822883077 1107 90-91 -1.8214219618680048 1107 92-93 -0.9931275582792516 1107 94-95 -1.8234272915785006 1107 96-97 -2.896191189901259 1107 98-99 -3.47825605270965 1107 100-101 -4.17210624146044 1108 1 0.37456171951489736 1108 2 0.5730315229931726 1108 3 0.3892153625163175 1108 4 0.9945729779145651 1108 5 0.9324485085287222 1108 6 -0.004761272548030604 1108 7 0.22183378118666752 1108 8 0.5544459570141029 1108 9 0.03615008265398956 1108 10-11 0.01329533860719323 1108 12-13 0.9365581961354792 1108 14-15 0.5134086079170359 1108 16-17 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2.393992408419443 2311 42-43 2.4691273140504038 2311 44-45 2.9114914876246587 2311 46-47 2.8680376287734646 2311 48-49 2.3522483339043205 2311 50-51 2.5051060717180853 2311 52-53 2.232485293242892 2311 54-55 1.1886986781732247 2311 56-57 0.8155274109200583 2311 58-59 0.6150027534924689 2311 60-61 1.3275776837029731 2311 62-63 0.581141096618147 2311 64-65 1.3220109213018425 2311 66-67 1.1067996331608718 2311 68-69 1.5096468787506332 2311 70-71 1.0060813814219038 2311 72-73 1.543268684162829 2311 74-75 2.0532158726556418 2311 76-77 2.1770111277910864 2311 78-79 1.8283529831075462 2311 80-81 2.243438118623274 2311 82-83 0.8444270753972596 2311 84-85 0.7233090400887257 2311 86-87 0.8275611918967059 2311 88-89 0.5420966574783961 2311 90-91 -0.19575918350108878 2311 92-93 0.4193856972880887 2311 94-95 0.3017052640948812 2311 96-97 0.19871867224084028 2311 98-99 0.2985201933136814 2311 100-101 -0.30519213753679253 2312 1 0.3392676018678422 2312 2 -0.35932141818329555 2312 3 -0.30490228454250357 2312 4 -0.11425055149720009 2312 5 0.2736249791169527 2312 6 0.5687681392166724 2312 7 -0.13993092469569035 2312 8 0.1279753687788059 2312 9 0.40379714147751855 2312 10-11 -0.06023407315751683 2312 12-13 0.504205254959011 2312 14-15 0.6810556667405692 2312 16-17 -0.17186152113277586 2312 18-19 -0.05888511837069643 2312 20-21 0.639319215667328 2312 22-23 1.209273819402 2312 24-25 1.3113373677728148 2312 26-27 1.522304332564815 2312 28-29 0.822662084525895 2312 30-31 1.0310582857780872 2312 32-33 1.4177119498367219 2312 34-35 1.4574340377923605 2312 36-37 0.4083631512940329 2312 38-39 1.9362899411097416 2312 40-41 2.0980813610736675 2312 42-43 1.6857700687132393 2312 44-45 3.001520182029246 2312 46-47 2.3113661797060345 2312 48-49 3.6104979752242556 2312 50-51 3.1245465308285567 2312 52-53 2.90931456153557 2312 54-55 2.912515033983844 2312 56-57 3.285039606042009 2312 58-59 2.568015665974535 2312 60-61 2.29386175830842 2312 62-63 3.004383564336944 2312 64-65 3.602498726179892 2312 66-67 3.390874238612806 2312 68-69 3.727006993528253 2312 70-71 3.5100268620531807 2312 72-73 4.282866962498552 2312 74-75 3.2590982255968157 2312 76-77 3.819765790631834 2312 78-79 3.8957848338966414 2312 80-81 4.099966095667746 2312 82-83 4.633164521595248 2312 84-85 4.379334865052858 2312 86-87 5.167231206243908 2312 88-89 4.505511291624739 2312 90-91 4.180496196699771 2312 92-93 3.562499040186225 2312 94-95 3.3651916342526995 2312 96-97 4.507900881709993 2312 98-99 6.034531671075516 2312 100-101 4.866256929895059 2313 1 0.5190061639593431 2313 2 0.21747596743761832 2313 3 0.46143758473854035 2313 4 0.02790631124790366 2313 5 -0.1119959359157221 2313 6 -0.5325390503258092 2313 7 -0.2559439965911139 2313 8 0.1655570681252172 2313 9 -0.8249610284571247 2313 10-11 -0.3339268836150282 2313 12-13 -0.4189973594200751 2313 14-15 0.10507527458369736 2313 16-17 -0.3409791681916019 2313 18-19 -0.19532302686742753 2313 20-21 -0.6351905882542397 2313 22-23 -0.4173601675261054 2313 24-25 -0.11595021392653138 2313 26-27 -0.40008128835021495 2313 28-29 0.35370783615988444 2313 30-31 -0.1838109952676703 2313 32-33 -0.21055602401948903 2313 34-35 -0.06707576612920008 2313 36-37 -0.9854276983791763 2313 38-39 -2.22057280398829 2313 40-41 -2.531820599710649 2313 42-43 -2.75377241494688 2313 44-45 -3.009917726467485 2313 46-47 -2.6265423170259936 2313 48-49 -3.1362340509195334 2313 50-51 -2.680192031262944 2313 52-53 -4.007352105131094 2313 54-55 -3.650393462748184 2313 56-57 -3.3538492828468804 2313 58-59 -3.8968536150712154 2313 60-61 -3.308262424698114 2313 62-63 -2.6631000957937765 2313 64-65 -1.6336123849312187 2313 66-67 -2.024975434589809 2313 68-69 -1.8873720914390724 2313 70-71 -3.5765744451363624 2313 72-73 -2.8347800963249767 2313 74-75 -3.2384507940110225 2313 76-77 -3.77827342505444 2313 78-79 -3.608300133423622 2313 80-81 -3.363269198449899 2313 82-83 -3.294123060104095 2313 84-85 -3.050403697038643 2313 86-87 -2.687343957154784 2313 88-89 -3.5917109305974826 2313 90-91 -2.7435234111433644 2313 92-93 -3.0951806983758665 2313 94-95 -2.5661809147669103 2313 96-97 -2.346674281688699 2313 98-99 -2.125599047878733 2313 100-101 -3.225246338078799 2314 1 -0.2084571484096287 2314 2 -0.014704326063426976 2314 3 -0.5956902978610401 2314 4 -0.23467230510429715 2314 5 -0.44113639713165753 2314 6 -1.1698556121706716 2314 7 -0.5017511244737136 2314 8 -0.03517668449533318 2314 9 -0.32705746451582485 2314 10-11 -0.4631197557324356 2314 12-13 -0.3115550114116843 2314 14-15 -0.4035725241584416 2314 16-17 -0.5367338851727297 2314 18-19 -0.8025033203684728 2314 20-21 -0.3678949907699547 2314 22-23 -1.6125907754925635 2314 24-25 -0.37512212168334 2314 26-27 -0.6183726929623745 2314 28-29 -0.868514386062337 2314 30-31 -0.8082868862529935 2314 32-33 -1.1646964852354245 2314 34-35 -1.0356292252487052 2314 36-37 -0.45817402961606746 2314 38-39 0.2872888312318338 2314 40-41 -0.023172800968502827 2314 42-43 -0.07505124094269888 2314 44-45 -0.1336074539308001 2314 46-47 0.9856170121144743 2314 48-49 0.3957365989756454 2314 50-51 1.3279316584645109 2314 52-53 1.234786259648775 2314 54-55 1.5018602060149178 2314 56-57 1.4878697947212522 2314 58-59 1.1248969090378012 2314 60-61 1.2999660868322884 2314 62-63 2.2774135310406045 2314 64-65 3.121366650708193 2314 66-67 2.636712196437447 2314 68-69 1.7530891244938438 2314 70-71 1.3427127666036789 2314 72-73 2.2029557527316257 2314 74-75 0.4022724764292249 2314 76-77 0.5361919837505909 2314 78-79 0.33640636552815906 2314 80-81 -0.7712880663744279 2314 82-83 -0.539144024464683 2314 84-85 -0.256902648820617 2314 86-87 0.006576378274985473 2314 88-89 0.03381317841719067 2314 90-91 -0.44242278221254594 2314 92-93 -0.3134721029880261 2314 94-95 -1.6495142481002425 2314 96-97 -2.402753946258933 2314 98-99 -2.385557119157557 2314 100-101 -2.224198120049449 2315 1 -0.15877161381843408 2315 2 -0.12696847700682312 2315 3 0.1392153625163175 2315 4 0.04457297791456938 2315 5 -0.08421815813794353 2315 6 -0.10476127254803203 2315 7 -0.6614995521466653 2315 8 -0.02888737631923277 2315 9 -0.047183250679346145 2315 10-11 -0.028371328059471068 2315 12-13 -0.04677513719785509 2315 14-15 0.2884086079170274 2315 16-17 0.04235416514173096 2315 18-19 -0.17310080464520183 2315 20-21 0.38147607841243314 2315 22-23 0.3965287213627846 2315 24-25 0.2618275638512486 2315 26-27 -0.5973035105724378 2315 28-29 -0.29351438606233415 2315 30-31 0.13841122695455965 2315 32-33 0.667221753758291 2315 34-35 0.34125756720413136 2315 36-37 0.4534611905097208 2315 38-39 0.0627605293450415 2315 40-41 -0.06515393304398032 2315 42-43 -0.1843279705024372 2315 44-45 0.07897116242140356 2315 46-47 -0.5126534281370994 2315 48-49 -0.1890118286973106 2315 50-51 -0.9524142534851698 2315 52-53 -1.140685438464427 2315 54-55 -0.09483790719262686 2315 56-57 0.960039606042006 2315 58-59 0.6337019404843396 2315 60-61 0.7556264641907724 2315 62-63 0.7146776819840035 2315 64-65 0.5941653928465591 2315 66-67 0.8722467876324167 2315 68-69 0.7848501307831519 2315 70-71 0.21786999930808193 2315 72-73 -0.41811342965831244 2315 74-75 0.11154920598897533 2315 76-77 0.47172657494555637 2315 78-79 -0.41107791120139936 2315 80-81 -0.6966025317832347 2315 82-83 -0.3330119489929828 2315 84-85 0.027374080739132722 2315 86-87 0.5598782650674359 2315 88-89 0.7638446249580717 2315 90-91 1.3231432555232985 2315 92-93 1.374263746068575 2315 94-95 1.2421524185664232 2315 96-97 0.4672146072001908 2315 98-99 0.6355120632323796 2315 100-101 0.7691981063656428 2316 1 -1.1996318288721959 2316 2 -1.6382587995874687 2316 3 -1.510784637483681 2316 4 -1.7489754091822078 2316 5 -1.586906330180959 2316 6 -1.851535466096415 2316 7 -1.8410694446197837 2316 8 -1.421360494598801 2316 9 -1.1654628205718183 2316 10-11 -1.2447691775218388 2316 12-13 -1.2908611587032297 2316 14-15 -2.1301397791797427 2316 16-17 -2.0546888456109613 2316 18-19 -1.909928761634447 2316 20-21 -1.6244379000821922 2316 22-23 -2.1588476227232363 2316 24-25 -1.6540326512025132 2316 26-27 -1.7903142632606048 2316 28-29 -2.5539982570300808 2316 30-31 -2.893040385948673 2316 32-33 -3.2620793215105337 2316 34-35 -3.2821295295700637 2316 36-37 -3.139549562178459 2316 38-39 -3.7340136642033457 2316 40-41 -4.6272507072375255 2316 42-43 -4.919543024265884 2316 44-45 -4.075329912847412 2316 46-47 -4.119373858244629 2316 48-49 -4.508097850202688 2316 50-51 -4.030102425528181 2316 52-53 -4.010040277174106 2316 54-55 -4.1311282297732745 2316 56-57 -4.263347490732183 2316 58-59 -4.686728167042542 2316 60-61 -4.076631600325353 2316 62-63 -4.066773930919222 2316 64-65 -4.357178693174948 2316 66-67 -3.62667794355038 2316 68-69 -3.4769778262060953 2316 70-71 -3.11331279639084 2316 72-73 -3.157360741486265 2316 74-75 -2.8500099337959703 2316 76-77 -3.55784331752756 2316 78-79 -3.427744577868065 2316 80-81 -3.0769788758692513 2316 82-83 -2.8241409812510483 2316 84-85 -3.622088284852264 2316 86-87 -4.247379799448691 2316 88-89 -3.2767467728913893 2316 90-91 -3.435727712218636 2316 92-93 -3.59025238296368 2316 94-95 -3.31080457068089 2316 96-97 -3.0034843175309938 2316 98-99 -3.2671761088106344 2316 100-101 -3.4340277000859665 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 311.0 3 43.0 4 39.0 5 48.0 6 59.0 7 71.0 8 66.0 9 113.0 10 117.0 11 182.0 12 303.0 13 444.0 14 492.0 15 761.0 16 1052.0 17 1475.0 18 1216.0 19 700.0 20 675.0 21 662.0 22 691.0 23 710.0 24 721.0 25 752.0 26 718.0 27 828.0 28 850.0 29 1009.0 30 1251.0 31 1429.0 32 1947.0 33 2458.0 34 3534.0 35 5317.0 36 7850.0 37 11111.0 38 9138.0 39 1062.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 45.22393777378038 19.64722930095438 15.481603624310864 19.64722930095438 2 15.17805117618659 21.201325186874655 41.9531523132502 21.66747132368855 3 18.55965365082008 27.66297560569478 31.44450919990009 22.332861543585047 4 11.345699157481102 17.396516700522827 39.183789003962836 32.07399513803323 5 11.387325585267574 38.46947950314696 34.56658563388724 15.576609277698225 6 27.61099647207615 38.42774412567397 18.085602076815547 15.875657325434334 7 25.11399567315693 36.20569146280579 21.64919287735064 17.031119986686637 8 25.13813074157902 34.10165091199574 20.538210624417523 20.222007722007724 9 26.36133670616429 17.05665024630542 21.651577686060445 34.93043536146984 10-11 23.51878973471358 27.93579203142163 27.17771194621043 21.36770628765436 12-13 25.714190611711647 26.14607517620724 28.775661349243993 19.364072862837123 14-15 21.925240488632962 26.536131544785807 29.572612588622977 21.966015377958257 16-17 18.382799563998237 30.619139146135478 29.79955567759167 21.198505612274616 18-19 18.243310702875398 27.0725173056443 35.68373935037274 19.00043264110756 20-21 19.596815122344896 25.89002687344521 35.6934264058639 18.819731598345992 22-23 18.984898281815536 26.153014103257476 36.02196613554104 18.840121479385946 24-25 18.658057644944417 26.41200159755042 36.184517073820146 18.74542368368502 26-27 18.19127338081608 26.756473407441923 36.15789123344206 18.89436197829994 28-29 17.626500911156047 27.293908152142254 36.58437138553966 18.495219551162037 30-31 17.89283604197352 26.839254062960283 36.17262068219454 19.09528921287166 32-33 17.796046062703855 27.29065433002729 35.95320508553551 18.960094521733343 34-35 18.575874579768996 26.88146989315315 35.475817994208306 19.066837532869556 36-37 18.210939710115987 27.273559316392927 35.867738338908026 18.647762634583064 38-39 18.104065094763467 27.564603890377228 35.329550559928116 19.001780454931193 40-41 18.02291238550047 27.818497965839413 35.38108272253051 18.777506926129604 42-43 18.02744011515197 27.49419664029154 34.920000998427476 19.558362246129015 44-45 18.0005158456124 27.843182933830317 34.22302834654841 19.93327287400887 46-47 18.220924234103805 28.059008536768008 33.88414623999467 19.83592098913351 48-49 18.618061391757294 27.90504164621107 33.49420447831984 19.982692483711798 50-51 18.524989807719507 28.06579637071612 33.34664569968965 20.062568121874712 52-53 18.696433623458535 28.431992544392486 32.61329028607565 20.258283546073326 54-55 18.317523714428358 28.073722749209516 33.07871526044267 20.530038275919456 56-57 18.519442919183348 28.04955157323749 32.76843208705636 20.662573420522804 58-59 18.820495757777408 27.33821327566129 32.86391615371818 20.977374812843124 60-61 18.40047255361986 26.880646932561277 33.753473435498094 20.965407078320773 62-63 18.156935824695097 26.2840884511073 34.39793015091263 21.16104557328497 64-65 18.089549258747777 26.116869935608396 34.442022595297914 21.351558210345917 66-67 18.236497953479088 26.084822468470264 34.190209976373495 21.48846960167715 68-69 18.03425985241142 25.88707060790855 33.74237722443614 22.336292315243888 70-71 18.338838368291 25.84842711305983 33.69330992653482 22.11942459211435 72-73 17.749490411414783 25.4003910312409 34.05549315695328 22.79462540039103 74-75 17.674809465171233 25.52750690584751 33.75378573568077 23.043897893300496 76-77 17.601297944920542 25.28829353523588 33.66003827273484 23.450370247108747 78-79 17.54070672024894 25.142067909708878 33.957350506285934 23.35987486375625 80-81 17.45717447190862 24.637886136925214 33.59401648959624 24.310922901569924 82-83 17.72863786490961 23.85254698380213 34.02717115498207 24.391643996306186 84-85 17.47656009517392 23.685327077145782 34.09705410104741 24.74105872663289 86-87 17.6928132305107 23.760679494538586 33.47059655427721 25.075910720673505 88-89 17.195104703112392 23.394926662063114 33.59235255455627 25.817616080268223 90-91 17.239744091049012 24.179069709398583 33.81309328696578 24.768092912586628 92-93 16.99251247920133 24.72712146422629 33.43594009983361 24.844425956738768 94-95 16.984334312265492 25.33964508856147 31.83969916555046 25.83632143362258 96-97 17.495049669700652 25.44136978551342 31.662146196981546 25.40143434780438 98-99 18.117678381256656 24.97670394036209 30.928015175718848 25.977602502662407 100-101 19.4140352044721 23.513459554786547 31.388713273217316 25.683791967524037 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 117.0 1 82.0 2 58.0 3 73.0 4 58.0 5 45.5 6 83.0 7 190.5 8 328.0 9 368.5 10 296.0 11 231.5 12 227.0 13 241.5 14 242.0 15 243.0 16 259.5 17 290.0 18 308.0 19 310.0 20 333.0 21 361.5 22 386.5 23 442.5 24 523.5 25 602.5 26 688.5 27 792.0 28 879.5 29 942.0 30 1015.5 31 1129.0 32 1239.5 33 1335.5 34 1427.0 35 1477.5 36 1603.5 37 1749.0 38 1770.0 39 1751.0 40 1746.5 41 1825.5 42 1917.5 43 1991.5 44 2036.5 45 2059.5 46 2117.5 47 2246.5 48 2446.5 49 2346.5 50 1990.5 51 1791.5 52 1691.0 53 1541.5 54 1412.5 55 1277.0 56 1057.5 57 876.0 58 770.5 59 649.0 60 528.5 61 381.5 62 277.0 63 214.0 64 144.0 65 111.5 66 86.5 67 66.5 68 47.0 69 28.0 70 18.5 71 19.0 72 14.0 73 6.0 74 3.5 75 5.5 76 7.0 77 4.0 78 2.5 79 2.0 80 1.0 81 1.5 82 1.5 83 0.5 84 0.5 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.27572460759073164 2 0.2292168424549456 3 0.24914874179885393 4 0.24416576696287684 5 0.24416576696287684 6 0.18769205215513662 7 0.19101403537912134 8 0.19433601860310604 9 0.19433601860310604 10-11 0.19765800182709076 12-13 0.19848849763308696 14-15 0.19765800182709076 16-17 0.1885225479611328 18-19 0.18104808570716718 20-21 0.18021758990117098 22-23 0.18686155634914042 24-25 0.18769205215513662 26-27 0.18769205215513662 28-29 0.1935055227971099 30-31 0.19848849763308696 32-33 0.18769205215513662 34-35 0.19765800182709076 36-37 0.18603106054314425 38-39 0.1793870940951748 40-41 0.1768956066771863 42-43 0.18353957312515573 44-45 0.18187858151316336 46-47 0.18603106054314425 48-49 0.19184453118511752 50-51 0.18353957312515573 52-53 0.1926750269911137 54-55 0.19101403537912134 56-57 0.17606511087119012 58-59 0.15779420313927414 60-61 0.17606511087119012 62-63 0.17274312764720537 64-65 0.17274312764720537 66-67 0.17108213603521302 68-69 0.17523461506519392 70-71 0.18021758990117098 72-73 0.17855659828917864 74-75 0.18437006893115188 76-77 0.18270907731915953 78-79 0.18353957312515573 80-81 0.1768956066771863 82-83 0.17357362345320157 84-85 0.17357362345320157 86-87 0.16859064861722448 88-89 0.1768956066771863 90-91 0.17523461506519392 92-93 0.17440411925919774 94-95 0.17523461506519392 96-97 0.1793870940951748 98-99 0.18104808570716718 100-101 0.16443816958724358 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 60205.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 79.32065443069513 #Duplication Level Percentage of deduplicated Percentage of total 1 91.73280284786934 72.76305954654929 2 5.149199036750079 8.168756747778422 3 1.449062925348131 3.4482185864961377 4 0.6240184273898021 1.9799020014948925 5 0.30782117055805674 1.220828834814384 6 0.1905559627264161 0.9069014201478283 7 0.11935922940006281 0.6627356531849514 8 0.07747879803161972 0.4916535171497384 9 0.07119673332635326 0.5082634332696621 >10 0.24290650193696997 3.6309276638152976 >50 0.016752172547377236 1.0248318245992858 >100 0.012564129410532929 1.4849265011211694 >500 0.0062820647052664646 3.7089942695789384 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 983 1.6327547545884895 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 719 1.1942529690225065 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 531 0.8819865459679428 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 198 0.32887633917448716 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 168 0.2790465908147164 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 165 0.27406361597873935 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 147 0.24416576696287684 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 110 0.18270907731915953 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 106 0.17606511087119012 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 100 0.16609916119923596 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 100 0.16609916119923596 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 82 0.13620131218337347 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 77 0.12789635412341166 No Hit NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 75 0.12457437089942697 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 64 0.106303463167511 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 63 0.10464247155551865 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0016609916119923595 0.0 3 0.0 0.0 0.0 0.0016609916119923595 0.0 4 0.0 0.0 0.0 0.0016609916119923595 0.0 5 0.0 0.0 0.0 0.0016609916119923595 0.0 6 0.0 0.0 0.0 0.0016609916119923595 0.0 7 0.0 0.0 0.0 0.0016609916119923595 0.0 8 0.0 0.0 0.0 0.0016609916119923595 0.0 9 0.0 0.0 0.0 0.0016609916119923595 0.0 10-11 0.0 0.0 0.0 0.0016609916119923595 0.0 12-13 0.0 0.0 0.0 0.0016609916119923595 0.0 14-15 0.0 0.0 0.0 0.0016609916119923595 0.0 16-17 0.0 0.0 0.0 0.0016609916119923595 0.0 18-19 0.0 0.0 0.0 0.0016609916119923595 0.0 20-21 0.0 0.0 0.0 0.0016609916119923595 0.0 22-23 0.0 0.0 0.0 0.003321983223984719 0.0 24-25 0.0 0.0 0.0 0.008304958059961797 0.0 26-27 0.0 0.0 0.0 0.010796445477950337 0.0 28-29 0.0 0.0 0.0 0.017440411925919774 0.0 30-31 0.0 0.0 0.0 0.03654181546383191 0.0 32-33 0.0 0.0 0.0 0.05896520222572876 0.0 34-35 0.0 0.0 0.0 0.07474462253965618 0.0 36-37 0.0 0.0 0.0 0.0863715638236027 0.0 38-39 0.0 0.0 0.0 0.12872684992940786 0.0 40-41 0.0 0.0 0.0 0.21925089278299142 0.0 42-43 0.0 0.0 0.0 0.41441740719209363 0.0 44-45 0.0 0.0 0.0 0.5597541732414251 0.0 46-47 0.0 0.0 0.0 0.9733410846275227 0.0 48-49 0.0 0.0 0.0 1.399385433103563 0.0 50-51 0.0 0.0 0.0 1.6601611161863632 0.0 52-53 0.0 0.0 0.0 1.954156631509011 0.0 54-55 0.0 0.0 0.0 2.2481521468316585 0.0 56-57 0.0 0.0 0.0 3.0711734905738726 0.0 58-59 0.0 0.0 0.0 4.0362096171414334 0.0 60-61 0.0 0.0 0.0 5.055227971098747 0.0 62-63 0.0 0.0 0.0 6.239514990449298 0.0 64-65 0.0 0.0 0.0 7.437920438501786 0.0 66-67 0.0 0.0 0.0 8.848932812889295 0.0 68-69 0.0 0.0 0.0 10.224233867618969 0.0 70-71 0.0 0.0 0.0 11.585416493646708 0.0 72-73 0.0 0.0 0.0 13.029648700274063 0.0 74-75 0.0 0.0 0.0 14.59347230296487 0.0 76-77 0.0 0.0 0.0 16.58084876671373 0.0 78-79 0.0 0.0 0.0 18.196993605182293 0.0 80-81 0.0 0.0 0.0 20.21260692633502 0.0 82-83 0.0 0.0 0.0 22.613570301469977 0.0 84-85 0.0 0.0 0.0 25.39988373058716 0.0 86-87 0.0 0.0 0.0 27.392243169171998 0.0 88-89 0.0 0.0 0.0 29.370484179054895 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TGTCATA 25 0.0049976925 57.0015 7 CCTACCC 25 0.0049976925 57.0015 3 GTATGTC 25 0.0049976925 57.0015 1 CTACCCT 25 0.0049976925 57.0015 4 GGTATCA 335 0.0 55.299957 1 ATACAGT 35 2.7663258E-4 54.287136 6 GTATCAA 610 0.0 41.27158 1 ACAGTCT 60 8.1960054E-5 39.58437 8 ACTGGTC 65 1.313061E-4 36.53942 8 TCAACGC 750 0.0 32.934196 4 ATCAACG 755 0.0 32.71609 3 TATCAAC 765 0.0 32.28843 2 CAACGCA 770 0.0 32.078766 5 GCACTGG 60 0.0039209835 31.667496 6 AACGCAG 795 0.0 31.070002 6 CGGTGGT 40 4.4693163E-4 29.688278 94-95 CATGGGG 65 0.0057999394 29.231537 4 GCAGAGT 875 0.0 28.229313 9 ACGCAGA 880 0.0 27.529133 7 CGCAGAG 885 0.0 27.3736 8 >>END_MODULE