FastQCFastQC Report
Thu 26 May 2016
SRR937692_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937692_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences60205
Sequences flagged as poor quality0
Sequence length101
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6711.1145253716468733No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5800.9633751349555684No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4310.715887384768707No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3670.6095839216011959No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT2880.47836558425379955No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1500.24914874179885393No Hit
GTCCCAGGGTGTGCTTGTCAAAGAGATATTCTGCCATGCCAGCTTCAGGG1300.2159289095590067No Hit
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA1170.19433601860310604No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1050.17440411925919774No Hit
CTACTGGAACTGCACAAACTGGCTACTGACAAGAATGATCCCCACTTATG700.11626941283946517No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT620.10298147994352627No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGACAG250.005002925656.986555
ACAGGCC459.56356E-442.2122543
CAGCCCT500.001605539937.991039
GTACAGG550.002552860934.566011
CACAGCA550.002563301734.53734
TACAGGC550.002563301734.53732
AGCACTG550.002563301734.53735
GTATCAA7750.033.1164671
GGTATCA5200.031.9901771
GCACTGT600.00392607431.6591916
TATTATA650.005807456629.2238712
TCAACGC8950.028.1218514
TATCAAC9150.028.026172
CAACGCA9000.027.965625
ATCAACG9000.027.965623
GCATGTT350.00833803327.15900680-81
AACGCAG9350.026.9187776
GAGTACT5400.026.82236912-13
GTACTTT5400.026.82236914-15
TAGAAGA908.861241E-426.382664
Produced by FastQC (version 0.11.5)