##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937692_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 60205 Sequences flagged as poor quality 0 Sequence length 101 %GC 41 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.33341084627523 33.0 31.0 34.0 31.0 34.0 2 32.51357860642804 34.0 31.0 34.0 31.0 34.0 3 32.588804916535175 34.0 31.0 34.0 31.0 34.0 4 36.209351382775516 37.0 35.0 37.0 35.0 37.0 5 36.1048750103812 37.0 35.0 37.0 35.0 37.0 6 36.22313761315505 37.0 36.0 37.0 35.0 37.0 7 36.18684494643302 37.0 36.0 37.0 35.0 37.0 8 36.188107300058135 37.0 36.0 37.0 35.0 37.0 9 37.842106137364006 39.0 38.0 39.0 35.0 39.0 10-11 37.81059712648451 39.0 38.0 39.0 35.0 39.0 12-13 37.755526949588905 39.0 37.0 39.0 35.0 39.0 14-15 38.93837721119509 40.0 38.0 41.0 35.5 41.0 16-17 38.917797525122495 40.0 38.0 41.0 35.5 41.0 18-19 38.966555933892536 40.0 38.0 41.0 35.5 41.0 20-21 38.94012125238768 40.0 38.0 41.0 35.0 41.0 22-23 38.79902001494892 40.0 38.0 41.0 35.0 41.0 24-25 38.700348808238516 40.0 38.0 41.0 35.0 41.0 26-27 38.58979320654431 40.0 38.0 41.0 35.0 41.0 28-29 38.431002408437834 40.0 38.0 41.0 34.0 41.0 30-31 38.37854829333112 40.0 37.5 41.0 35.0 41.0 32-33 38.16926335022008 40.0 37.0 41.0 34.0 41.0 34-35 37.93735570135371 40.0 37.0 41.0 34.0 41.0 36-37 37.687210364587656 40.0 36.0 41.0 33.0 41.0 38-39 37.47119840544805 40.0 36.0 41.0 33.0 41.0 40-41 37.155103396727846 40.0 36.0 41.0 33.0 41.0 42-43 36.79993356033552 40.0 35.0 41.0 32.5 41.0 44-45 36.26991944190682 40.0 35.0 41.0 30.5 41.0 46-47 35.63101901835396 39.5 35.0 41.0 28.0 41.0 48-49 35.03713146748609 39.0 35.0 41.0 23.5 41.0 50-51 34.654048667054234 39.0 35.0 41.0 21.0 41.0 52-53 34.35536085042771 39.0 35.0 40.5 17.5 41.0 54-55 34.07615646540985 39.0 35.0 40.0 11.0 41.0 56-57 33.711784735487086 38.0 34.0 40.0 4.5 41.0 58-59 33.397026825014535 38.0 34.0 40.0 2.0 41.0 60-61 32.99171165185616 38.0 33.5 40.0 2.0 41.0 62-63 32.60204301968275 37.0 33.0 40.0 2.0 41.0 64-65 32.16805913130139 36.5 33.0 40.0 2.0 41.0 66-67 31.67425463001412 36.0 32.0 39.0 2.0 41.0 68-69 31.166082551283118 35.5 31.5 39.0 2.0 40.0 70-71 30.727979403704012 35.0 31.0 38.5 2.0 40.0 72-73 30.22144340171082 35.0 30.5 38.0 2.0 40.0 74-75 29.76137364006312 35.0 30.0 37.0 2.0 39.5 76-77 28.5872851092102 34.0 28.5 36.0 2.0 38.5 78-79 29.03253882567893 34.0 29.0 36.0 2.0 39.0 80-81 28.813869279960137 34.0 29.0 36.0 2.0 37.5 82-83 28.481272319574785 34.0 29.0 35.5 2.0 37.0 84-85 28.094244664064448 34.0 29.0 35.0 2.0 37.0 86-87 27.704700606261937 34.0 28.0 35.0 2.0 36.0 88-89 27.336566730338014 34.0 27.0 35.0 2.0 36.0 90-91 26.928934473880908 34.0 26.0 35.0 2.0 35.0 92-93 26.583755502034712 33.5 25.0 35.0 2.0 35.0 94-95 26.168474379204383 33.0 24.5 35.0 2.0 35.0 96-97 25.74146665559339 33.0 23.0 35.0 2.0 35.0 98-99 25.114243003072836 33.0 17.5 35.0 2.0 35.0 100-101 23.044639149572294 30.5 2.0 33.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.26128297557217195 1101 2 0.12471267443429213 1101 3 0.20336140157109384 1101 4 0.2777232601925945 1101 5 0.2652751214451996 1101 6 0.10743441114433239 1101 7 0.34597282232314086 1101 8 0.36650198296714365 1101 9 0.22068177493549257 1101 10-11 0.28127365692684947 1101 12-13 0.14063860979538845 1101 14-15 0.3285116503031986 1101 16-17 -0.027967099253949357 1101 18-19 0.29834444538349203 1101 20-21 0.4441749037452496 1101 22-23 0.5434746633047993 1101 24-25 0.6283552635869043 1101 26-27 0.8918353660042087 1101 28-29 0.5231607897248978 1101 30-31 0.8035771796029962 1101 32-33 0.9215685684228774 1101 34-35 0.9296297172558994 1101 36-37 1.2489214926540129 1101 38-39 0.8686407079634151 1101 40-41 1.1870210576097406 1101 42-43 0.7056370224268846 1101 44-45 1.0166677965256383 1101 46-47 0.9104155755386287 1101 48-49 1.307897368815219 1101 50-51 1.372461942827428 1101 52-53 1.0655341142431496 1101 54-55 1.199935314053775 1101 56-57 1.2218063267638755 1101 58-59 0.6387832404624731 1101 60-61 1.062237338149096 1101 62-63 1.3193693189467766 1101 64-65 1.2082736544717676 1101 66-67 1.6019120045870956 1101 68-69 1.1635108708580155 1101 70-71 0.9633147832673998 1101 72-73 1.092968222660165 1101 74-75 0.5929281380274567 1101 76-77 1.0593289130661638 1101 78-79 0.7992840212050112 1101 80-81 0.8005952492431341 1101 82-83 0.3463335229870026 1101 84-85 0.47843967667799703 1101 86-87 1.2855354330994224 1101 88-89 1.4422603474555267 1101 90-91 1.6428566006848406 1101 92-93 1.585296571554423 1101 94-95 0.9188877702789071 1101 96-97 0.9377104431343533 1101 98-99 1.1506609206378897 1101 100-101 2.0624068693328397 1102 1 -0.06880360451440737 1102 2 -0.0705254208038042 1102 3 -0.0011840529743594175 1102 4 -0.26556678309744797 1102 5 0.07739633356641207 1102 6 -0.29927554556562797 1102 7 -0.22112674477642713 1102 8 0.3210474375125969 1102 9 0.49405839831212006 1102 10-11 -0.13907266341947633 1102 12-13 -0.38046528630850673 1102 14-15 -0.08263553584398409 1102 16-17 -0.5074043286911873 1102 18-19 0.3263747484137909 1102 20-21 0.13183724140759523 1102 22-23 0.23546600529613926 1102 24-25 -0.43885252862089175 1102 26-27 -0.2195282703594259 1102 28-29 -0.4402591236950215 1102 30-31 -0.6375483615225477 1102 32-33 -0.6096002627459569 1102 34-35 0.2324435700697478 1102 36-37 -0.16330794457542908 1102 38-39 -0.979086564763854 1102 40-41 -0.5529140073253274 1102 42-43 0.03377555056541581 1102 44-45 -0.012444757586919764 1102 46-47 0.13877055389360038 1102 48-49 -1.710067999150148 1102 50-51 -1.3610878407223623 1102 52-53 -1.2430156693066365 1102 54-55 -1.5338309197124609 1102 56-57 -2.4817651018075537 1102 58-59 -1.9468228201435807 1102 60-61 -2.0225029215911654 1102 62-63 -1.5764099018324416 1102 64-65 -0.9793886831905709 1102 66-67 -1.1328282551531643 1102 68-69 -1.1796709473238032 1102 70-71 -1.0586549137022985 1102 72-73 -0.2746724699805334 1102 74-75 -0.07904155894224374 1102 76-77 -0.15160182286457058 1102 78-79 -0.630369658448668 1102 80-81 -0.6141233654754821 1102 82-83 0.2725239991774764 1102 84-85 0.17465179789011742 1102 86-87 0.3926782902422765 1102 88-89 -0.0786270984319195 1102 90-91 -0.3515156936874497 1102 92-93 0.427071463329316 1102 94-95 0.37191807330921023 1102 96-97 0.5127104431343525 1102 98-99 0.7993622193391907 1102 100-101 0.6198744018003737 1103 1 -0.0728440085548101 1103 2 -0.3816365319149142 1103 3 0.12002806823775813 1103 4 -0.041324358855021615 1103 5 -0.6013915452214675 1103 6 -0.5457401920302729 1103 7 -0.13021765386733364 1103 8 0.022057538522702202 1103 9 0.11830082255453789 1103 10-11 0.022543498196689882 1103 12-13 -0.2319804378236512 1103 14-15 -0.347282000490452 1103 16-17 -0.7033639246507803 1103 18-19 -0.06554444350539512 1103 20-21 0.2671907767611259 1103 22-23 -0.22716025733012657 1103 24-25 -0.5196606094289749 1103 26-27 -0.5720535228846799 1103 28-29 -1.2331884166243157 1103 30-31 -0.4900736140477946 1103 32-33 -1.1752568284025173 1103 34-35 -2.3806877430615643 1103 36-37 -2.0451261263936047 1103 38-39 -3.5841370698143677 1103 40-41 -2.438772593183913 1103 42-43 -1.853093136303272 1103 44-45 -1.8043639495061115 1103 46-47 -1.9268860117629671 1103 48-49 -1.6817851708673217 1103 50-51 -0.7822999619344841 1103 52-53 -0.4197833460743112 1103 54-55 -0.8429218288033695 1103 56-57 -0.37065399069644656 1103 58-59 -0.441772315093079 1103 60-61 -1.1548261539143994 1103 62-63 -1.1127735381960804 1103 64-65 -0.1814088852107716 1103 66-67 -0.4429292652541754 1103 68-69 -0.9392669069197623 1103 70-71 -0.6172407722881559 1103 72-73 -0.8534603487684116 1103 74-75 -1.1376274175281011 1103 76-77 -1.161702832965581 1103 78-79 -1.1919858200648292 1103 80-81 -0.47876983012194785 1103 82-83 -0.8052537786003029 1103 84-85 -1.5687825455442272 1103 86-87 -1.518432820868835 1103 88-89 -0.1422634620682821 1103 90-91 -0.7888894310611896 1103 92-93 0.09878863504648905 1103 94-95 -0.3200011186099836 1103 96-97 -0.8095117790878668 1103 98-99 -1.6056882857113166 1103 100-101 -0.476085194159225 1104 1 -0.03633607204687195 1104 2 -0.018144468422853777 1104 3 0.35812330633299894 1104 4 0.13486611733545573 1104 5 -0.1156772595071871 1104 6 -0.4520893983794778 1104 7 -0.15402717767685914 1104 8 -0.20492658846142575 1104 9 0.13734844160215687 1104 10-11 -0.37348824783506274 1104 12-13 -0.38912329496650955 1104 14-15 0.3106545074460527 1104 16-17 0.41250909122224044 1104 18-19 -0.5290365069974641 1104 20-21 -0.2105870010166484 1104 22-23 -0.027953908123777182 1104 24-25 -0.2823590221273875 1104 26-27 0.24302584219469026 1104 28-29 -0.6554106388465328 1104 30-31 -0.6607085346827191 1104 32-33 -0.2570028601485461 1104 34-35 -0.16560837798220263 1104 36-37 -0.9832213644888483 1104 38-39 -0.5063592920365849 1104 40-41 -0.41416941858074097 1104 42-43 -0.7467439299540644 1104 44-45 -0.6678560129981719 1104 46-47 0.3032727183957675 1104 48-49 1.1769449878628393 1104 50-51 1.6819857523512383 1104 52-53 1.2500579237669598 1104 54-55 0.9856495997680597 1104 56-57 1.5372825172400653 1104 58-59 1.5256880023672394 1104 60-61 1.841999242911001 1104 62-63 1.718178842756302 1104 64-65 1.4463688925670013 1104 66-67 2.250721528396621 1104 68-69 2.6932727756199206 1104 70-71 3.195457640410254 1104 72-73 3.2060634607554057 1104 74-75 3.229832899932216 1104 76-77 2.981947960685215 1104 78-79 2.4302364021573943 1104 80-81 2.2648809635288494 1104 82-83 1.572523999177477 1104 84-85 2.0796301528684715 1104 86-87 1.9402973378613275 1104 88-89 1.460117490312669 1104 90-91 1.4642851721134136 1104 92-93 1.1091060953639484 1104 94-95 2.097459198850334 1104 96-97 3.2293771098010176 1104 98-99 2.1030418730188423 1104 100-101 1.473121155047128 1105 1 -0.31958730357396803 1105 2 -0.29811162770035793 1105 3 -0.20181101222200937 1105 4 -0.1056921749469737 1105 5 0.10435558121530875 1105 6 0.12016019111148779 1105 7 -0.16470041248802403 1105 8 -0.030049741170785182 1105 9 -0.2288256142270697 1105 10-11 0.07848219551469526 1105 12-13 -0.12968158724893897 1105 14-15 0.4875838998926909 1105 16-17 0.36406902554079323 1105 18-19 0.038286974119124295 1105 20-21 0.19171184955806098 1105 22-23 -0.06202615771326947 1105 24-25 -0.46831961326039107 1105 26-27 -0.12109566847855291 1105 28-29 -0.09793937447872736 1105 30-31 0.1919187231169488 1105 32-33 0.015164627536179864 1105 34-35 -0.9377758656669286 1105 36-37 0.41004628739949567 1105 38-39 -0.4546351541055529 1105 40-41 -0.4441365778582451 1105 42-43 -0.5718670826634238 1105 44-45 -2.807429083605726 1105 46-47 -1.091636969617376 1105 48-49 -1.14078900228494 1105 50-51 -1.8742539849229871 1105 52-53 -1.8148025031624364 1105 54-55 -1.6153356219068193 1105 56-57 -1.6852133776696263 1105 58-59 -1.233343196319133 1105 60-61 -2.3804966519986905 1105 62-63 -1.585187331299526 1105 64-65 -0.5047805327203534 1105 66-67 -2.4011668131468973 1105 68-69 -2.2373511981075005 1105 70-71 -3.0467426879969715 1105 72-73 -5.451161498193695 1105 74-75 -5.701612091857601 1105 76-77 -5.023388656720371 1105 78-79 -4.713441758762148 1105 80-81 -4.516317722842253 1105 82-83 -4.237820828408729 1105 84-85 -3.876445380793271 1105 86-87 -4.1590458476887555 1105 88-89 -4.6043324275855255 1105 90-91 -4.936782151367705 1105 92-93 -4.23531098181175 1105 94-95 -5.336859356157873 1105 96-97 -4.2562550741070275 1105 98-99 -5.096876025174918 1105 100-101 -4.356928106036619 1106 1 0.22937821366740963 1106 2 0.04947457919620035 1106 3 0.253361401571091 1106 4 0.5853423078116435 1106 5 0.451941788111867 1106 6 0.3298153635252845 1106 7 0.3897823461326695 1106 8 0.06650198296714649 1106 9 0.5849674892212064 1106 10-11 0.3336546093077928 1106 12-13 0.021352895509679115 1106 14-15 0.2682735550651003 1106 16-17 0.3144138531270002 1106 18-19 -0.2899888879498391 1106 20-21 0.9027463323166813 1106 22-23 1.1772841871143171 1106 24-25 0.9447838350154711 1106 26-27 0.9768353660042024 1106 28-29 0.5979226944867975 1106 30-31 0.2788152748410937 1106 32-33 0.11585428270859666 1106 34-35 0.9215344791606626 1106 36-37 1.0948738736063959 1106 38-39 0.9336407079634128 1106 40-41 1.2434496290383095 1106 42-43 1.1446846414745053 1106 44-45 1.4111916060494494 1106 46-47 0.8442250993481437 1106 48-49 0.24265927357712513 1106 50-51 0.5443667047321838 1106 52-53 1.282438876147907 1106 54-55 1.2170781711966256 1106 56-57 1.5582348981924454 1106 58-59 0.9204499071291394 1106 60-61 0.7829516238633829 1106 62-63 1.5072264618039242 1106 64-65 1.9697022259003347 1106 66-67 1.972626290301374 1106 68-69 1.918510870858018 1106 70-71 -0.655018550065936 1106 72-73 -0.9201270154350745 1106 74-75 -0.495405195305878 1106 76-77 -0.266147277410024 1106 78-79 -0.7030969311759421 1106 80-81 -0.5032142745663926 1106 82-83 -1.7874760008225223 1106 84-85 -1.9798936566553387 1106 86-87 -1.8673217097577215 1106 88-89 -1.3022634620682858 1106 90-91 -1.4333338755056353 1106 92-93 -1.7856558093979586 1106 94-95 -2.004445563054425 1106 96-97 -1.507289556865647 1106 98-99 -1.467910507933535 1106 100-101 0.3416925836185527 1107 1 0.3580738658413267 1107 2 -0.07922107297771674 1107 3 0.1490135754841404 1107 4 0.21664665563773156 1107 5 -0.5897973423229175 1107 6 -0.10322811473558602 1107 7 -0.4780437408238569 1107 8 -0.48132410398937964 1107 9 0.22844575009077062 1107 10-11 0.05974156582953327 1107 12-13 -0.2421253653598825 1107 14-15 -0.2830307927609823 1107 16-17 0.2831095053009136 1107 18-19 -0.040423670558539015 1107 20-21 0.34013763666450814 1107 22-23 0.012936361027364285 1107 24-25 -0.15347703454974493 1107 26-27 0.755965800786818 1107 28-29 0.9605313901389678 1107 30-31 0.9422935357106539 1107 32-33 0.6358542827085927 1107 34-35 0.7893605661171748 1107 36-37 0.950526047519439 1107 38-39 0.7762494036155871 1107 40-41 0.4704061507774355 1107 42-43 0.23772811973537245 1107 44-45 0.4511916060494485 1107 46-47 1.5320511863046633 1107 48-49 -0.7764711612054853 1107 50-51 -0.6156332952678127 1107 52-53 -0.42799590646079366 1107 54-55 -0.8994435679338082 1107 56-57 -0.2991564061553831 1107 58-59 -0.5369413972186834 1107 60-61 -0.842265767440967 1107 62-63 -2.434512668630859 1107 64-65 -2.3833412523605304 1107 66-67 -3.4221563183942685 1107 68-69 -4.439749998707203 1107 70-71 -3.277627245718108 1107 72-73 -3.1940400589133375 1107 74-75 -4.919753021392832 1107 76-77 -4.853973364366549 1107 78-79 -4.403096931175941 1107 80-81 -4.527562100653348 1107 82-83 -4.166606435605132 1107 84-85 -4.197284961003167 1107 86-87 -4.281234753235985 1107 88-89 -3.764002592503065 1107 90-91 -3.268116484201286 1107 92-93 -4.067394939832738 1107 94-95 -3.2948803456631204 1107 96-97 -2.469898252517819 1107 98-99 -2.0496496383683187 1107 100-101 -0.6200465468162264 1108 1 -0.30062178633258796 1108 2 0.1794745791961958 1108 3 -0.3466385984289033 1108 4 -0.3746576921883573 1108 5 -0.21805821188813468 1108 6 0.20981536352527996 1108 7 -0.7802176538673322 1108 8 -0.5334980170328549 1108 9 -0.26503251077879497 1108 10-11 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0.7614283918523022 2310 98-99 0.8695253895023605 2310 100-101 0.2734874554134272 2311 1 0.23718309171619012 2311 2 0.358742871879123 2311 3 -0.03444347647768353 2311 4 0.06924474683603421 2311 5 0.15145398323381443 2311 6 0.1512787781594298 2311 7 -0.057046922160019164 2311 8 0.3055263732110447 2311 9 0.5264309038553563 2311 10-11 0.14828875564926136 2311 12-13 0.020865090631630778 2311 14-15 0.07168818921145004 2311 16-17 0.8207553165416286 2311 18-19 0.23635257546479238 2311 20-21 0.08616096646302651 2311 22-23 0.5002110163826146 2311 24-25 0.5686862740398695 2311 26-27 0.18756707332128286 2311 28-29 -0.8464675494156424 2311 30-31 0.39881527484109114 2311 32-33 0.7334152583183453 2311 34-35 0.0025100889167575247 2311 36-37 0.7182885077527388 2311 38-39 1.1033968055243903 2311 40-41 0.17613255586758214 2311 42-43 0.8490748853769432 2311 44-45 0.06094770361042379 2311 46-47 0.6354446115432708 2311 48-49 1.048025127235661 2311 50-51 -0.28636500258488695 2311 52-53 -0.21219527019356121 2311 54-55 0.2765903663185796 2311 56-57 0.33286904453391486 2311 58-59 0.31459624859255797 2311 60-61 0.8814882092292322 2311 62-63 0.5384459739990461 2311 64-65 0.7492144210222875 2311 66-67 0.7809189732282071 2311 68-69 0.4775352611019166 2311 70-71 0.9318107182267461 2311 72-73 0.4798729845649241 2311 74-75 -0.05442958554978006 2311 76-77 0.981657600638755 2311 78-79 0.8286103858972318 2311 80-81 1.2280052376287323 2311 82-83 1.2920361942994276 2311 84-85 1.5444965872470995 2311 86-87 1.5756051195105698 2311 88-89 2.038224342809766 2311 90-91 2.2764222220553414 2311 92-93 2.2226368735288737 2311 94-95 2.335554436945575 2311 96-97 0.6297836138660635 2311 98-99 1.155016321334756 2311 100-101 -0.5739171724790069 2312 1 0.16114291954976778 2312 2 0.029474579196197226 2312 3 0.6004202251005069 2312 4 0.14887171957634848 2312 5 0.25547119987656686 2312 6 0.3392271282311654 2312 7 0.2815470520150214 2312 8 0.13120786532008566 2312 9 -0.05032662842585012 2312 10-11 0.2924781387195665 2312 12-13 0.2278234837449702 2312 14-15 0.0788617903592197 2312 16-17 -0.046174382167116335 2312 18-19 0.4576581708736853 2312 20-21 -0.4654889618009719 2312 22-23 -0.035656989356269264 2312 24-25 -0.4105102826315843 2312 26-27 0.3109530130630347 2312 28-29 -0.11619495257203027 2312 30-31 -0.10118472515890886 2312 32-33 -0.07002807023258129 2312 34-35 -0.33787728554522545 2312 36-37 -1.8921849499230206 2312 38-39 -1.226947527330701 2312 40-41 -0.9400797827264 2312 42-43 -0.41060947617255295 2312 44-45 -0.01939662924466745 2312 46-47 0.46363686405403115 2312 48-49 0.8891298618124139 2312 50-51 0.0020137635557162525 2312 52-53 1.0847918173243727 2312 54-55 1.057078171196629 2312 56-57 1.3741172511336188 2312 58-59 1.9892734365409126 2312 60-61 2.0023633885692647 2312 62-63 2.2048735206274515 2312 64-65 1.603231637665047 2312 66-67 1.6155674667719673 2312 68-69 2.3767461649756676 2312 70-71 3.387334391110535 2312 72-73 4.288696513976692 2312 74-75 4.016947745870592 2312 76-77 3.376205663766445 2312 78-79 2.5821971864711166 2312 80-81 2.5561974901394926 2312 82-83 2.4989945874127706 2312 84-85 2.328929872756426 2312 86-87 1.6573841725952185 2312 88-89 1.8459718320493614 2312 90-91 1.240195536259069 2312 92-93 1.2337559553079274 2312 94-95 1.232613260474988 2312 96-97 1.6509457372519982 2312 98-99 1.4867953744194047 2312 100-101 0.784045524795026 2313 1 0.16604488033407705 2313 2 0.2239190236406401 2313 3 0.1478058460155367 2313 4 -0.4802132477439116 2313 5 0.23749734366742103 2313 6 -0.19018463647471862 2313 7 -0.6024398760895551 2313 8 0.06094642741158651 2313 9 0.12941193366565074 2313 10-11 0.21976572041891274 2313 12-13 0.19024178439856598 2313 14-15 -1.2667264449348963 2313 16-17 -0.4783639246507789 2313 18-19 -0.8960999990609579 2313 20-21 -0.5716981121277627 2313 22-23 -0.5854935906634537 2313 24-25 -1.0224383872067477 2313 26-27 -0.4553868562180128 2313 28-29 -0.5026328610687614 2313 30-31 -3.712295836270023 2313 32-33 -2.6558123839580716 2313 34-35 -3.5584655208393414 2313 36-37 -3.3701261263936075 2313 38-39 -3.242470403147699 2313 40-41 -3.060994815406133 2313 42-43 -4.147537580747716 2313 44-45 -4.854363949506109 2313 46-47 -4.62133045620741 2313 48-49 -4.134562948645097 2313 50-51 -4.837855517490034 2313 52-53 -4.797561123852091 2313 54-55 -3.623477384358928 2313 56-57 -3.9706539906964444 2313 58-59 -3.3334389817597447 2313 60-61 -2.7770483761366194 2313 62-63 -2.7877735381960775 2313 64-65 -3.6925199963218844 2313 66-67 -3.3901514874763983 2313 68-69 -2.2531557958086523 2313 70-71 -1.8977963278437144 2313 72-73 -2.645127015435076 2313 74-75 -2.1987385286392147 2313 76-77 -2.964480610743358 2313 78-79 -3.250319153398163 2313 80-81 -3.7648809412330557 2313 82-83 -4.191364889711409 2313 84-85 -3.0076714344331172 2313 86-87 -2.829543931979945 2313 88-89 -3.283930128734948 2313 90-91 -4.180556097727855 2313 92-93 -3.712322476064621 2313 94-95 -2.8172233408322036 2313 96-97 -2.7706228901989824 2313 98-99 -2.2779105079335373 2313 100-101 -1.3066407497147772 2314 1 -0.4204331070873053 2314 2 -0.14977070382266788 2314 3 -0.3390914286175857 2314 4 0.29704042101919015 2314 5 0.21496065603638925 2314 6 0.1683059295630187 2314 7 0.0207257423590832 2314 8 0.017445379193560484 2314 9 0.09062786657970179 2314 10-11 -0.03993029635257983 2314 12-13 0.03720195211344901 2314 14-15 0.2780848758198218 2314 16-17 0.1366780040703972 2314 18-19 0.2598224328048744 2314 20-21 0.5472746342034753 2314 22-23 0.33615211164262604 2314 24-25 -0.1280463536637697 2314 26-27 0.49796744147590744 2314 28-29 0.4137717510905645 2314 30-31 0.4554190484259948 2314 32-33 0.8056656034633107 2314 34-35 0.7528552338776393 2314 36-37 1.2265719868139442 2314 38-39 1.2766595758879404 2314 40-41 2.0155251007364257 2314 42-43 1.239778981097146 2314 44-45 0.9794934928419039 2314 46-47 0.7125269861406025 2314 48-49 1.0098290848978806 2314 50-51 1.5070082141661487 2314 52-53 2.381684159166781 2314 54-55 2.396700812706065 2314 56-57 2.2106877283811244 2314 58-59 1.623846133544241 2314 60-61 1.345593133297342 2314 62-63 2.4268491033133586 2314 64-65 1.6806456221267467 2314 66-67 2.2956451582259056 2314 68-69 2.1328504934995287 2314 70-71 1.8823399405001027 2314 72-73 2.0270427958856807 2314 74-75 2.733651408467704 2314 76-77 2.6825319678729933 2314 78-79 1.8799219367485875 2314 80-81 2.6635781782637977 2314 82-83 2.657429659554836 2314 84-85 2.840861060325796 2314 86-87 2.854942441185674 2314 88-89 3.0407554058562454 2314 90-91 3.5345906527962505 2314 92-93 3.350570605696383 2314 94-95 3.1751770784550075 2314 96-97 1.208936858228693 2314 98-99 1.4437876052740108 2314 100-101 1.646975602486478 2315 1 -0.11728845299925439 2315 2 -0.03719208747047276 2315 3 0.15336140157109668 2315 4 -0.024657692188355895 2315 5 0.2819417881118653 2315 6 0.1598153635252828 2315 7 0.13644901279933208 2315 8 0.19983531630047935 2315 9 0.018300822554543572 2315 10-11 0.5253212759744628 2315 12-13 0.44301956217634597 2315 14-15 0.9166068883984408 2315 16-17 0.5244138531270011 2315 18-19 0.23167777871682205 2315 20-21 0.13941299898334591 2315 22-23 0.25061752044765484 2315 24-25 0.6497838350154765 2315 26-27 0.40850203267087437 2315 28-29 -0.18041063884653852 2315 30-31 0.20714860817442826 2315 32-33 0.35252094937526124 2315 34-35 0.6748678124939929 2315 36-37 -1.411792793060279 2315 38-39 0.16864070796341224 2315 40-41 0.5751162957049729 2315 42-43 0.824684641474505 2315 44-45 1.3428582727161142 2315 46-47 2.0258917660148157 2315 48-49 2.1126592735771297 2315 50-51 2.5677000380655244 2315 52-53 2.4607722094812416 2315 54-55 2.423744837863296 2315 56-57 2.409901564859112 2315 58-59 2.472116573795816 2315 60-61 2.956284957196715 2315 62-63 2.603893128470588 2315 64-65 2.829702225900334 2315 66-67 3.5626262903013775 2315 68-69 3.4635108708580127 2315 70-71 3.14664811660073 2315 72-73 2.7965396512315905 2315 74-75 2.7762614713607867 2315 76-77 2.2855193892566383 2315 78-79 2.705236402157393 2315 80-81 1.9517857254336093 2315 82-83 1.8391906658441428 2315 84-85 2.203439676677995 2315 86-87 2.476011623575612 2315 88-89 2.216069871265052 2315 90-91 2.6083327911610326 2315 92-93 2.8876775239353734 2315 94-95 2.5272211036122414 2315 96-97 1.862710443134354 2315 98-99 1.9887561587331284 2315 100-101 2.226692583618558 2316 1 -0.024815334719683335 2316 2 0.045603611454261284 2316 3 -0.35309021133213037 2316 4 -0.2069157567044826 2316 5 0.012586949402184189 2316 6 -0.06115237841019905 2316 7 -0.5173144280608852 2316 8 -0.520594791226408 2316 9 -0.4118067043271765 2316 10-11 -0.38731313262768907 2316 12-13 -1.1099374270709603 2316 14-15 -0.9643070900961845 2316 16-17 -1.0352635662278402 2316 18-19 -0.7003114685950038 2316 20-21 -1.5546730225220244 2316 22-23 -0.8359416193372979 2316 24-25 -0.4171516488554943 2316 26-27 -0.25735818238289454 2316 28-29 -0.41078698293256366 2316 30-31 -0.7866685961266526 2316 32-33 -0.783500556001087 2316 34-35 -0.8084655208393414 2316 36-37 -1.0435132231677997 2316 38-39 -1.522488324294649 2316 40-41 -1.4471955322520103 2316 42-43 -1.465637939170655 2316 44-45 -2.0004212971763593 2316 46-47 -2.049645868393789 2316 48-49 -2.037340726422876 2316 50-51 -1.688213940429101 2316 52-53 -1.983044994819835 2316 54-55 -2.7735669900936912 2316 56-57 -2.549507037291427 2316 58-59 -3.0211629960966633 2316 60-61 -2.8093064406527475 2316 62-63 -3.3321283769057572 2316 64-65 -3.1073945482932146 2316 66-67 -3.3825350000212 2316 68-69 -3.138908483980696 2316 70-71 -3.640179840388516 2316 72-73 -3.310449596080236 2316 74-75 -3.4379858404671673 2316 76-77 -3.48195372902293 2316 78-79 -3.7095485440791656 2316 80-81 -3.5619239519857437 2316 82-83 -3.927476000822523 2316 84-85 -3.141183979235983 2316 86-87 -2.8169991291125633 2316 88-89 -3.1777473330360273 2316 90-91 -3.0510758109895058 2316 92-93 -3.0214622610108606 2316 94-95 -3.0515423372479766 2316 96-97 -3.469547621381775 2316 98-99 -4.503716959546441 2316 100-101 -3.630565480897573 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1.0 3 0.0 4 0.0 5 1.0 6 4.0 7 9.0 8 26.0 9 46.0 10 68.0 11 135.0 12 211.0 13 334.0 14 453.0 15 640.0 16 911.0 17 1254.0 18 1158.0 19 668.0 20 558.0 21 520.0 22 538.0 23 529.0 24 619.0 25 622.0 26 609.0 27 681.0 28 759.0 29 871.0 30 1042.0 31 1346.0 32 1575.0 33 2133.0 34 3185.0 35 5045.0 36 8021.0 37 12980.0 38 11496.0 39 1157.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.52659035403964 21.019753783788275 15.292984000930371 19.160671861241713 2 15.639897018520058 22.539656174736315 40.35711319657836 21.463333610165268 3 19.171165185615813 28.034216427207042 31.392741466655593 21.401876920521552 4 12.344489660327215 18.99343908313263 37.49522464911552 31.16684660742463 5 12.130374111236627 40.03588278290916 33.26300750880457 14.570735597049639 6 28.62386844946433 38.5217174653268 17.495224649115524 15.359189436093349 7 25.874927331616977 37.15970434349307 20.19101403537912 16.774354289510836 8 26.097500207623952 35.369155385765296 19.39706004484677 19.136284361763973 9 27.422971513993854 18.089859646208787 20.21925089278299 34.26791794701437 10-11 24.376712897599866 29.167012706585833 26.118262602773857 20.338011793040444 12-13 26.179362811866714 27.045613102554732 27.54476595461945 19.230258130959104 14-15 22.43086122415082 27.775101735736236 28.04086039365501 21.753176646457938 16-17 19.58059961797193 31.547213686570885 28.20695955485425 20.66522714060294 18-19 19.05240428535836 28.79245909808155 33.292915870774856 18.862220745785233 20-21 20.761737079454193 27.444792332799047 33.31810745043975 18.475363137307014 22-23 20.24333527115688 27.764305290258285 33.28378041690889 18.70857902167594 24-25 20.137862303795366 28.198654596794288 33.410846275226305 18.252636824184037 26-27 19.340907580891635 28.69659823267557 33.70623214404358 18.25626204238921 28-29 18.604554590302808 29.36813780044184 33.7366908625816 18.290616746673756 30-31 19.09309857985217 28.613902499792378 33.39423635910639 18.898762561249065 32-33 19.362179220994935 29.094759571464163 32.72734822689146 18.815712980649447 34-35 19.303214018769204 29.16950419400382 32.7821609500872 18.745120837139773 36-37 19.756664728843116 28.833153392575365 33.00307283448218 18.407109044099325 38-39 19.235943858483516 29.366331700024915 32.76887301719126 18.628851424300308 40-41 18.858068266755254 29.713478946931314 32.436674694792785 18.99177809152064 42-43 19.47540656821542 29.886709081546204 31.478097642817986 19.15978670742039 44-45 19.612158458599787 29.652852753093594 31.357030146997754 19.37795864130886 46-47 19.65451374470559 30.634498795781077 30.597126484511257 19.113860975002076 48-49 20.280707582426707 30.1951665144091 30.043185781911802 19.480940121252388 50-51 20.018270907731917 30.501619466821694 30.01744041192592 19.462669213520474 52-53 19.889544057802507 31.296403953160034 29.297400548127232 19.516651440910223 54-55 19.256706253633418 31.07964454779503 29.173656673033797 20.489992525537744 56-57 19.75832572045511 30.59795698031725 29.73507183788722 19.90864546134042 58-59 20.044846773523794 29.618802425047754 29.757495224649116 20.578855576779336 60-61 19.344738809069014 29.946848268416243 30.474213105223818 20.234199817290925 62-63 19.09143758824018 29.29075658167926 30.913545386595796 20.70426044348476 64-65 18.844780333859315 29.200232538825677 31.036458765883236 20.918528361431772 66-67 18.573208205298563 28.92450793123495 30.80142845278631 21.700855410680177 68-69 18.68532513910805 28.91703346898098 30.430196827506023 21.96744456440495 70-71 18.927829914458933 29.335603355203055 30.15696370733328 21.579603023004733 72-73 18.538327381446724 28.520056473714806 30.396976995266172 22.544639149572294 74-75 17.984386678847272 28.848102317083296 29.909475957146412 23.258035046923013 76-77 17.866456274395816 27.90216759405365 30.516568391329624 23.714807740220913 78-79 18.360601278963543 28.015945519475128 29.7176314259613 23.90582177560003 80-81 17.668798272568722 27.65883232289677 29.568142180881985 25.10422722365252 82-83 17.523607039340913 27.272047770515496 29.771860907407255 25.43248428273634 84-85 17.041773939041608 27.2826177227805 30.03571131965784 25.63989701852006 86-87 17.19375467153891 27.623951499044928 29.052404285358357 26.129889544057804 88-89 16.260277385599203 27.559172826177225 29.089776596628187 27.090773191595378 90-91 16.452521158462137 27.598607984983513 29.45656597536565 26.492304881188694 92-93 16.398548257161128 27.69855573180961 28.602988198360563 27.2999078126687 94-95 15.897350718378872 28.455277800847107 27.373972261440077 28.273399219333946 96-97 15.962129391246574 29.85383273814467 26.636491985715473 27.54754588489328 98-99 16.322700130388927 29.914707372372497 25.495602488186098 28.266990009052478 100-101 16.001096363726973 28.071894881974785 25.175667369889865 30.75134138440838 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 179.0 1 111.5 2 43.5 3 53.0 4 63.5 5 64.0 6 84.0 7 123.5 8 170.5 9 215.0 10 219.5 11 200.5 12 208.5 13 236.5 14 248.0 15 252.5 16 257.0 17 271.0 18 272.0 19 282.0 20 307.0 21 316.5 22 357.0 23 432.5 24 493.0 25 545.5 26 606.5 27 695.0 28 797.5 29 897.0 30 985.5 31 1089.0 32 1192.0 33 1296.5 34 1407.0 35 1476.5 36 1622.0 37 1738.5 38 1712.5 39 1711.5 40 1723.5 41 1821.5 42 1922.0 43 2004.0 44 2063.5 45 2053.0 46 2088.0 47 2248.0 48 2495.5 49 2384.5 50 2098.0 51 1876.0 52 1743.0 53 1678.5 54 1507.0 55 1338.0 56 1156.0 57 998.5 58 903.0 59 773.0 60 578.5 61 431.0 62 360.5 63 255.0 64 151.5 65 108.0 66 91.5 67 66.5 68 41.5 69 27.0 70 17.5 71 11.5 72 11.0 73 8.5 74 6.5 75 6.5 76 5.0 77 2.5 78 0.5 79 2.0 80 2.0 81 1.0 82 1.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.02325388256789303 2 0.0 3 0.0 4 0.0 5 0.014948924507931236 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.004982974835977079 14-15 0.0 16-17 0.0 18-19 0.0 20-21 8.304958059961797E-4 22-23 0.0 24-25 0.0 26-27 0.0016609916119923595 28-29 0.003321983223984719 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.009965949671954157 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 8.304958059961797E-4 84-85 0.0 86-87 0.0 88-89 0.0 90-91 0.007474462253965618 92-93 0.0024914874179885393 94-95 0.0 96-97 0.0 98-99 8.304958059961797E-4 100-101 0.009965949671954157 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 60205.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 77.75932231542231 #Duplication Level Percentage of deduplicated Percentage of total 1 90.7081063761615 70.53400880325555 2 5.818647869272668 9.049082302134375 3 1.49951938481256 3.498048334855909 4 0.6942219374132222 2.159289095590067 5 0.3567232724554096 1.38692799601362 6 0.19438214247570224 0.9069014201478283 7 0.16447719747944037 0.8952744788638818 8 0.09398696998825162 0.5846690474213105 9 0.07903449749012069 0.5531102067934557 >10 0.3545872049556766 4.964703928245163 >50 0.017088539997863934 0.7524292002325388 >100 0.01495247249813094 2.6376546798438665 >500 0.004272134999465983 2.077900506602442 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 671 1.1145253716468733 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 580 0.9633751349555684 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 431 0.715887384768707 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 367 0.6095839216011959 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 288 0.47836558425379955 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 150 0.24914874179885393 No Hit GTCCCAGGGTGTGCTTGTCAAAGAGATATTCTGCCATGCCAGCTTCAGGG 130 0.2159289095590067 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 117 0.19433601860310604 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 105 0.17440411925919774 No Hit CTACTGGAACTGCACAAACTGGCTACTGACAAGAATGATCCCCACTTATG 70 0.11626941283946517 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 62 0.10298147994352627 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0016609916119923595 0.0 3 0.0 0.0 0.0 0.0016609916119923595 0.0 4 0.0 0.0 0.0 0.0016609916119923595 0.0 5 0.0 0.0 0.0 0.0016609916119923595 0.0 6 0.0 0.0 0.0 0.0016609916119923595 0.0 7 0.0 0.0 0.0 0.0016609916119923595 0.0 8 0.0 0.0 0.0 0.0016609916119923595 0.0 9 0.0 0.0 0.0 0.0016609916119923595 0.0 10-11 0.0 0.0 0.0 0.003321983223984719 0.0 12-13 0.0 0.0 0.0 0.003321983223984719 0.0 14-15 0.0 0.0 0.0 0.003321983223984719 0.0 16-17 0.0 0.0 0.0 0.003321983223984719 0.0 18-19 0.0 0.0 0.0 0.004982974835977079 0.0 20-21 0.0 0.0 0.0 0.004982974835977079 0.0 22-23 0.0 0.0 0.0 0.006643966447969438 0.0 24-25 0.0 0.0 0.0 0.011626941283946516 0.0 26-27 0.0 0.0 0.0 0.014118428701935054 0.0 28-29 0.0 0.0 0.0 0.020762395149904495 0.0 30-31 0.0 0.0 0.0 0.03986379868781663 0.0 32-33 0.0 0.0 0.0 0.06228718544971348 0.0 34-35 0.0 0.0 0.0 0.07889710156963708 0.0 36-37 0.0 0.0 0.0 0.09218503446557594 0.0 38-39 0.0 0.0 0.0 0.14035379121335437 0.0 40-41 0.0 0.0 0.0 0.22755585084295324 0.0 42-43 0.0 0.0 0.0 0.4235528610580517 0.0 44-45 0.0 0.0 0.0 0.5722116103313678 0.0 46-47 0.0 0.0 0.0 0.9924424881654348 0.0 48-49 0.0 0.0 0.0 1.4259612988954404 0.0 50-51 0.0 0.0 0.0 1.6883979735902335 0.0 52-53 0.0 0.0 0.0 1.9873764637488582 0.0 54-55 0.0 0.0 0.0 2.294659911967445 0.0 56-57 0.0 0.0 0.0 3.128477701187609 0.0 58-59 0.0 0.0 0.0 4.109293248069097 0.0 60-61 0.0 0.0 0.0 5.13910804750436 0.0 62-63 0.0 0.0 0.0 6.33917448716884 0.0 64-65 0.0 0.0 0.0 7.562494809401212 0.0 66-67 0.0 0.0 0.0 8.989286604102649 0.0 68-69 0.0 0.0 0.0 10.374553608504277 0.0 70-71 0.0 0.0 0.0 11.756498629681921 0.0 72-73 0.0 0.0 0.0 13.204052819533262 0.0 74-75 0.0 0.0 0.0 14.77618138028403 0.0 76-77 0.0 0.0 0.0 16.779337264346815 0.0 78-79 0.0 0.0 0.0 18.40627854829333 0.0 80-81 0.0 0.0 0.0 20.446806743625945 0.0 82-83 0.0 0.0 0.0 22.867702018104808 0.0 84-85 0.0 0.0 0.0 25.68059131301387 0.0 86-87 0.0 0.0 0.0 27.675442239016693 0.0 88-89 0.0 0.0 0.0 29.691886055975417 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TAGACAG 25 0.0050029256 56.98655 5 ACAGGCC 45 9.56356E-4 42.212254 3 CAGCCCT 50 0.0016055399 37.99103 9 GTACAGG 55 0.0025528609 34.56601 1 CACAGCA 55 0.0025633017 34.5373 4 TACAGGC 55 0.0025633017 34.5373 2 AGCACTG 55 0.0025633017 34.5373 5 GTATCAA 775 0.0 33.116467 1 GGTATCA 520 0.0 31.990177 1 GCACTGT 60 0.003926074 31.659191 6 TATTATA 65 0.0058074566 29.223871 2 TCAACGC 895 0.0 28.121851 4 TATCAAC 915 0.0 28.02617 2 CAACGCA 900 0.0 27.96562 5 ATCAACG 900 0.0 27.96562 3 GCATGTT 35 0.008338033 27.159006 80-81 AACGCAG 935 0.0 26.918777 6 GAGTACT 540 0.0 26.822369 12-13 GTACTTT 540 0.0 26.822369 14-15 TAGAAGA 90 8.861241E-4 26.38266 4 >>END_MODULE