FastQCFastQC Report
Thu 26 May 2016
SRR937691_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937691_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences354412
Sequences flagged as poor quality0
Sequence length101
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT26970.7609787478979266No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT18750.5290452919201382No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12990.3665225782422717No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT4970.14023227204496463No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4900.1382571696217961No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT4480.126406555082785No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN3990.1125808381206054No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT3820.10778416080719615No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA25500.035.5948641
GTAAGCG352.0554998E-433.9202494-95
GGTATCA16100.030.1070671
GTACATG17200.029.8393571
ATCAACG30700.029.4026493
TATCAAC30750.029.354842
TACATGG17100.029.171882
TCAACGC31050.028.9223044
CAACGCA31200.028.7832555
AACGCAG31850.028.1878646
ACATGGG16750.028.0796433
TAGGCCC856.386362E-427.9501254
TAAGGTG1205.3544954E-627.7172075
GGGTATG700.00836497827.1553961
ACGCGGC350.00839948427.1361994-95
ACGCAGA34800.025.6618777
CGCAGAG34700.025.4620448
CTAGACT1151.2765205E-424.7905464
GAGTACT18450.023.94228712-13
ACGCGAC500.001651979323.75256354-55
Produced by FastQC (version 0.11.5)