FastQCFastQC Report
Thu 26 May 2016
SRR937691_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937691_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences354412
Sequences flagged as poor quality0
Sequence length101
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT26830.7570285430515897No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20510.578705009988375No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16090.4539913998397345No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT10500.2962653634752774No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5860.16534428856810718No Hit
GCTAAGGGTAGTTTATTTTGGACACAGACATATTAGGGCATTGTCACCAC4390.12386713768156835No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3950.1114522081645091No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3730.10524474340597947No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACACG352.780344E-454.3053133
TGTTACG550.002572669434.5579262
CCTAGAC1001.292181E-633.2620053
AACCGAC300.003951144431.66469676-77
TATTCGT804.4779602E-429.698224
GTATCAA33850.028.7770021
GTATAGT1004.9314218E-528.510291
ACTATAC700.00836912527.1526573
GTGTAGC1658.346615E-825.9184441
GGTATCA23900.025.4485151
GAGTACT21650.024.90029512-13
GTACATG18800.024.5168271
TATCAAC39650.024.3278222
ATCAACG39750.024.1470813
TCAACGC39750.024.1470814
CAACGCA40100.023.9363175
GTACTTT22650.023.80094314-15
AGTACTT22400.023.7485212-13
TACATGG19850.023.4593492
AACGCAG41150.023.4311036
Produced by FastQC (version 0.11.5)