##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937691_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 354412 Sequences flagged as poor quality 0 Sequence length 101 %GC 42 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.2553779217408 33.0 31.0 34.0 31.0 34.0 2 32.55923614324571 34.0 31.0 34.0 31.0 34.0 3 32.65177533492094 34.0 31.0 34.0 31.0 34.0 4 36.24387718248818 37.0 35.0 37.0 35.0 37.0 5 36.18539157816327 37.0 35.0 37.0 35.0 37.0 6 36.24255386386466 37.0 37.0 37.0 35.0 37.0 7 36.194623771204135 37.0 36.0 37.0 35.0 37.0 8 36.17766892768868 37.0 36.0 37.0 35.0 37.0 9 37.87782298567769 39.0 38.0 39.0 35.0 39.0 10-11 37.812272157827614 39.0 38.0 39.0 35.0 39.0 12-13 37.72050325609742 39.0 37.0 39.0 35.0 39.0 14-15 38.94998053113326 40.0 38.0 41.0 36.0 41.0 16-17 38.92854784826699 40.0 38.0 41.0 36.0 41.0 18-19 38.93592203424264 40.0 38.0 41.0 35.5 41.0 20-21 38.868132286717156 40.0 38.0 41.0 35.0 41.0 22-23 38.80410510930781 40.0 38.0 41.0 35.0 41.0 24-25 38.82502285475661 40.0 38.0 41.0 35.0 41.0 26-27 38.68873373362076 40.0 38.0 41.0 35.0 41.0 28-29 38.53148736498764 40.0 38.0 41.0 34.0 41.0 30-31 38.550919551256726 40.0 38.0 41.0 35.0 41.0 32-33 38.45175106937688 40.0 38.0 41.0 35.0 41.0 34-35 38.30057249754523 40.0 38.0 41.0 34.0 41.0 36-37 38.147255171946775 40.0 38.0 41.0 34.0 41.0 38-39 38.002037177070754 40.0 37.5 41.0 34.0 41.0 40-41 37.803392661648026 40.0 37.0 41.0 33.0 41.0 42-43 37.55397672765031 40.0 37.0 41.0 33.0 41.0 44-45 37.182658882882066 40.0 37.0 41.0 32.5 41.0 46-47 36.70091024005959 40.0 37.0 41.0 31.0 41.0 48-49 36.26870280915996 40.0 36.0 41.0 30.0 41.0 50-51 35.96164210015462 39.5 36.0 41.0 29.0 41.0 52-53 35.74860332042933 39.0 36.0 41.0 28.0 41.0 54-55 35.519013182397885 39.0 35.0 40.5 28.0 41.0 56-57 35.25392057830999 39.0 35.0 40.0 27.0 41.0 58-59 34.999273444465764 38.5 35.0 40.0 26.0 41.0 60-61 34.65563101700845 38.0 34.5 40.0 26.0 41.0 62-63 34.318575556132416 38.0 34.0 40.0 25.0 41.0 64-65 33.91946943105764 37.5 34.0 40.0 23.5 41.0 66-67 33.50744613613534 37.0 33.5 39.5 22.0 41.0 68-69 33.06745539090099 36.0 33.0 39.0 21.0 40.5 70-71 32.64108438766182 36.0 32.5 39.0 19.5 40.0 72-73 32.14665981964493 35.0 32.0 38.0 17.5 40.0 74-75 31.66861026150356 35.0 31.5 37.5 13.5 39.5 76-77 30.42258303894902 34.0 30.0 36.0 9.5 39.0 78-79 30.954607913953254 35.0 31.0 36.0 7.5 39.0 80-81 30.73111237768473 35.0 31.0 36.0 7.0 38.5 82-83 30.34376375517759 34.0 31.0 36.0 2.0 37.0 84-85 29.97654706951232 34.0 31.0 35.0 2.0 37.0 86-87 29.627735516856088 34.0 30.0 35.0 2.0 36.5 88-89 29.233238434364523 34.0 30.0 35.0 2.0 36.0 90-91 28.758646716251143 34.0 29.0 35.0 2.0 35.5 92-93 28.34803985192375 34.0 29.0 35.0 2.0 35.0 94-95 27.935771079986004 34.0 29.0 35.0 2.0 35.0 96-97 27.443402029276662 33.5 27.5 35.0 2.0 35.0 98-99 26.676805525772266 33.0 25.5 35.0 2.0 35.0 100-101 24.116116271458075 31.0 13.0 33.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.22366525792144643 1101 2 0.22054794223711127 1101 3 0.15875871109374629 1101 4 0.008363781196855768 1101 5 0.09543100549524297 1101 6 0.017867296797170695 1101 7 0.05735199209310338 1101 8 -0.03505881046763193 1101 9 0.02058714666155481 1101 10-11 -0.06839228381678453 1101 12-13 0.07055339997078391 1101 14-15 0.0598540533390306 1101 16-17 0.1566885368297264 1101 18-19 0.04638870427992714 1101 20-21 0.06909009064513327 1101 22-23 0.15745776656072508 1101 24-25 0.1136932107584343 1101 26-27 -0.20110547471736595 1101 28-29 -0.05704920416850001 1101 30-31 0.01555309601895516 1101 32-33 0.0375168918724782 1101 34-35 -0.19163089028267422 1101 36-37 -0.08927161658362337 1101 38-39 -0.059979973828113486 1101 40-41 -0.23027016119730348 1101 42-43 0.04528974275358877 1101 44-45 0.11493041886595279 1101 46-47 0.12767348636101872 1101 48-49 -0.009025895596145972 1101 50-51 0.10842640927414493 1101 52-53 0.172940026855521 1101 54-55 -0.08601104964176898 1101 56-57 -0.15422031495148758 1101 58-59 -0.03654387889236688 1101 60-61 -0.06880796406057499 1101 62-63 -0.42652105784410566 1101 64-65 -0.368698810964986 1101 66-67 -0.5223568785561312 1101 68-69 5.539318940606108E-5 1101 70-71 -0.0022109198166262445 1101 72-73 0.05174441733654689 1101 74-75 0.07494134430199395 1101 76-77 -0.054692639961110956 1101 78-79 -0.24002439697001066 1101 80-81 -0.16251390546907984 1101 82-83 -0.2308054822682024 1101 84-85 0.12777632206324085 1101 86-87 -0.5089751077430336 1101 88-89 -0.3740812806315752 1101 90-91 -0.2382863216127049 1101 92-93 -0.6285630546023633 1101 94-95 -0.5231728011308832 1101 96-97 -0.6408328419667271 1101 98-99 -0.8039291790410452 1101 100-101 -0.8381388955242493 1102 1 0.27094878511103104 1102 2 0.13891706441625473 1102 3 0.10488904214057015 1102 4 -0.0022776013130041406 1102 5 0.036342129861481 1102 6 0.03957671125188966 1102 7 0.02697289543008452 1102 8 0.08732330173702252 1102 9 0.13732340275063137 1102 10-11 0.17353244362465148 1102 12-13 0.08197973459649432 1102 14-15 0.27104552366411383 1102 16-17 0.0938260919213505 1102 18-19 0.10643029224427636 1102 20-21 0.059150815700633075 1102 22-23 0.24386256160330788 1102 24-25 0.039251168805947145 1102 26-27 0.00246015473552319 1102 28-29 0.07418070602800952 1102 30-31 0.1328775484164808 1102 32-33 0.13253959127930415 1102 34-35 0.0012995700016489309 1102 36-37 -0.008317992962986409 1102 38-39 -0.04212490208473696 1102 40-41 0.2998976818619852 1102 42-43 0.3009107455040194 1102 44-45 0.32481501640712906 1102 46-47 0.21970756860909546 1102 48-49 0.36754400863555503 1102 50-51 0.4396161398636593 1102 52-53 0.32831291017725306 1102 54-55 0.4015270588514426 1102 56-57 0.5276566111843124 1102 58-59 0.47677131148440566 1102 60-61 0.36252011692825903 1102 62-63 0.47392050360998184 1102 64-65 0.48235704283094805 1102 66-67 0.029607365735877522 1102 68-69 0.013225605602833923 1102 70-71 0.23505794388751866 1102 72-73 0.21007899333813995 1102 74-75 0.004192098187406401 1102 76-77 0.19510646200395954 1102 78-79 0.6127670501585989 1102 80-81 0.42506106884911077 1102 82-83 0.3946253093943213 1102 84-85 0.3929617281672293 1102 86-87 0.21090261370044772 1102 88-89 0.1766982865884934 1102 90-91 0.302808717655612 1102 92-93 0.13525334530485011 1102 94-95 -0.284974007347536 1102 96-97 -0.3302651911068004 1102 98-99 -0.17617873996492506 1102 100-101 -0.11358045697833674 1103 1 0.30753323625050655 1103 2 0.11729017206317138 1103 3 0.1513806101527706 1103 4 0.1075820114031103 1103 5 0.0700888326979765 1103 6 0.07329216135376981 1103 7 0.08983464583324263 1103 8 0.09854303346354953 1103 9 -0.01284598707097473 1103 10-11 0.1878868246329901 1103 12-13 0.14545716387156205 1103 14-15 0.16731769747932645 1103 16-17 0.012809627980750804 1103 18-19 -0.11209195345222867 1103 20-21 -0.0984973535141691 1103 22-23 -0.01458505256293563 1103 24-25 0.07874524953800943 1103 26-27 -0.06262053982524662 1103 28-29 -0.09973763682698689 1103 30-31 0.021089641974661788 1103 32-33 0.010594546087851597 1103 34-35 -0.0954838985518549 1103 36-37 -0.26447478168200433 1103 38-39 -0.4924424298684116 1103 40-41 -0.5486453156502975 1103 42-43 -0.4624685501843473 1103 44-45 -0.1119075718193443 1103 46-47 -0.42899294268564603 1103 48-49 -0.8995543624719247 1103 50-51 -0.9788454755276845 1103 52-53 -0.9732259744751488 1103 54-55 -1.272810070647381 1103 56-57 -0.9571858125277615 1103 58-59 -0.87795035166112 1103 60-61 -0.6046707622736704 1103 62-63 -0.8405382615319681 1103 64-65 -0.4349888499346619 1103 66-67 -0.5320636554784812 1103 68-69 -0.5538427628794054 1103 70-71 -0.7664859412024256 1103 72-73 -0.7990718073569205 1103 74-75 -0.5880659743939454 1103 76-77 -0.5590470264262741 1103 78-79 -0.502861817367311 1103 80-81 -0.15697735951336966 1103 82-83 -0.18783190561198992 1103 84-85 -0.6588646816436281 1103 86-87 -0.4108142865283213 1103 88-89 -0.41570042329862034 1103 90-91 -0.46087685483198015 1103 92-93 -0.14831521694434358 1103 94-95 -0.19724133073833272 1103 96-97 -0.552865918670463 1103 98-99 -0.21587908874544226 1103 100-101 -0.7616965421357911 1104 1 0.36414144839763907 1104 2 0.22888127557044413 1104 3 0.15875871109374629 1104 4 0.15082409865716784 1104 5 0.0025738626380942264 1104 6 0.058343487273361916 1104 7 0.14227262701373888 1104 8 0.22009991969109421 1104 9 0.02892047999488767 1104 10-11 0.24490136697686893 1104 12-13 0.08841054282792982 1104 14-15 0.284854053339032 1104 16-17 0.03327583841702619 1104 18-19 0.2674204503116826 1104 20-21 0.17881231286735044 1104 22-23 0.11837046497342385 1104 24-25 -0.03749726543204446 1104 26-27 0.22786277925088427 1104 28-29 0.14215714503784938 1104 30-31 0.24154515951101985 1104 32-33 0.11866768552327045 1104 34-35 0.5000357763839887 1104 36-37 0.4964426691306656 1104 38-39 0.46898828014013816 1104 40-41 0.10207110864396185 1104 42-43 -0.031694384230540606 1104 44-45 0.3278272442627781 1104 46-47 0.40465761334515093 1104 48-49 0.2687518821816255 1104 50-51 0.4417597426074735 1104 52-53 0.365995582411081 1104 54-55 0.5445445059137768 1104 56-57 0.7195892088580322 1104 58-59 0.3604799306314419 1104 60-61 0.7155174327648197 1104 62-63 0.9113757675527197 1104 64-65 0.7636424588762836 1104 66-67 0.46236534366608595 1104 68-69 0.28557126620528805 1104 70-71 0.5924319373262321 1104 72-73 0.3362682268603585 1104 74-75 0.42513975700040874 1104 76-77 0.15522799495952455 1104 78-79 0.3294200474744322 1104 80-81 0.12954958659441118 1104 82-83 -0.03298802195074302 1104 84-85 0.06468108396800432 1104 86-87 0.4499534636855316 1104 88-89 0.6572679257176333 1104 90-91 0.5700470117206287 1104 92-93 0.12778615174684305 1104 94-95 0.1950811671230852 1104 96-97 0.13654811041422477 1104 98-99 0.39230097968911437 1104 100-101 0.4507499933646386 1105 1 0.25799723715864076 1105 2 0.3081974570193253 1105 3 0.06862668672001604 1105 4 0.037655513687639086 1105 5 0.031365331832411414 1105 6 -0.020075605482226422 1105 7 0.09325434671583821 1105 8 0.10446010926455784 1105 9 0.19396787335981713 1105 10-11 0.29926832703103656 1105 12-13 0.2950371508170946 1105 14-15 0.38830699849814465 1105 16-17 0.6840713698997618 1105 18-19 0.2571293196414004 1105 20-21 0.31725171706504796 1105 22-23 0.12241582719413913 1105 24-25 0.23791742651108194 1105 26-27 0.22720265738222878 1105 28-29 -0.20809674798855582 1105 30-31 -0.18669620812608656 1105 32-33 -0.07980049321742655 1105 34-35 -0.22211215861939593 1105 36-37 0.14951477475015906 1105 38-39 -0.09032959392892081 1105 40-41 0.08214050607118395 1105 42-43 -0.20330914387847798 1105 44-45 -0.07934946528359887 1105 46-47 -0.040154505815308994 1105 48-49 -0.3136787203911595 1105 50-51 -0.6275953691054568 1105 52-53 -0.6740518109538485 1105 54-55 -0.7124544785141183 1105 56-57 -0.8883661059693182 1105 58-59 -0.7977292772223237 1105 60-61 -0.7922009152378848 1105 62-63 -1.0360429866788294 1105 64-65 -0.7054502967093654 1105 66-67 -0.5525889555891652 1105 68-69 -0.6711484358935635 1105 70-71 -0.9749174194781034 1105 72-73 -0.8453583811288077 1105 74-75 -1.2486500195737271 1105 76-77 -1.1892794525692523 1105 78-79 -1.1392241637645633 1105 80-81 -0.7616826408937385 1105 82-83 -1.0361447585790415 1105 84-85 -1.0541239261877173 1105 86-87 -0.9089412553395135 1105 88-89 -0.8079656558666564 1105 90-91 -0.0798852834723327 1105 92-93 -0.3078350398577605 1105 94-95 -0.723080647365741 1105 96-97 -1.0114038191727772 1105 98-99 -0.21011100406173355 1105 100-101 0.13098018970857694 1106 1 0.1444644693257544 1106 2 0.22892677147581253 1106 3 -0.023111170616900267 1106 4 -0.01755624711171322 1106 5 0.17789233397567727 1106 6 0.12497224068555823 1106 7 0.13699510199099052 1106 8 0.18315724453186277 1106 9 0.4797166583388375 1106 10-11 0.3819350339468457 1106 12-13 -0.029139502667973716 1106 14-15 0.04336178764294374 1106 16-17 0.12979540165633097 1106 18-19 0.12465429926526639 1106 20-21 0.39111895526953333 1106 22-23 0.33977628844931473 1106 24-25 0.5121046453959792 1106 26-27 0.21755795668492084 1106 28-29 0.3739360349377634 1106 30-31 0.25920894477034295 1106 32-33 0.14554439161971544 1106 34-35 -0.3851211844895275 1106 36-37 -0.34148954197033987 1106 38-39 0.2180442401037439 1106 40-41 0.08381587661484957 1106 42-43 -0.05928765083654497 1106 44-45 -0.2775412725707014 1106 46-47 -0.12347023014893921 1106 48-49 -0.7610479358347462 1106 50-51 -0.8904172364644083 1106 52-53 -1.3355967742768229 1106 54-55 -1.4887698707923107 1106 56-57 -1.399746550923588 1106 58-59 -1.131535537976383 1106 60-61 -1.0786590913480083 1106 62-63 -1.1571979358139757 1106 64-65 -1.4633366130072432 1106 66-67 -1.8243612259426492 1106 68-69 -1.91508842442256 1106 70-71 -1.8300657878785032 1106 72-73 -1.7377422371978781 1106 74-75 -1.4523056479131498 1106 76-77 -1.3455568094080839 1106 78-79 -1.9884257214063688 1106 80-81 -2.130641496208135 1106 82-83 -2.274696393197331 1106 84-85 -2.2818384792713076 1106 86-87 -1.9687112314918984 1106 88-89 -1.8955528204152152 1106 90-91 -1.5242660001083088 1106 92-93 -1.2142406908373253 1106 94-95 -0.9876986326949293 1106 96-97 -0.14659565664673835 1106 98-99 -0.15850657263117895 1106 100-101 -1.1830989602295396 1107 1 0.2655852173946016 1107 2 0.21459556128473167 1107 3 0.19409305759830175 1107 4 0.11799735093680397 1107 5 0.0669355647657568 1107 6 -0.03063827564457 1107 7 0.22494739905021532 1107 8 0.11797226011663042 1107 9 0.4337077140374461 1107 10-11 0.41845759797991633 1107 12-13 0.09619929662731863 1107 14-15 0.4059786733998223 1107 16-17 0.029324470635870625 1107 18-19 0.10663017675544495 1107 20-21 0.04112234326248654 1107 22-23 0.19268657439591408 1107 24-25 0.4819176281849735 1107 26-27 0.2667685543268732 1107 28-29 0.2545431632749384 1107 30-31 0.4411272263803241 1107 32-33 0.2498606946418107 1107 34-35 0.323630001308004 1107 36-37 0.20157184846196685 1107 38-39 0.5832360005048756 1107 40-41 0.7318203487655452 1107 42-43 0.3506764364381496 1107 44-45 0.2779032321047268 1107 46-47 0.7673855768709856 1107 48-49 0.5301500584734242 1107 50-51 0.41687372437039016 1107 52-53 0.5995062207089532 1107 54-55 0.7964821958833852 1107 56-57 0.7229326739036352 1107 58-59 0.9570604777439016 1107 60-61 1.3103502595125462 1107 62-63 0.7879335183125988 1107 64-65 1.3169859239218766 1107 66-67 1.112859264638736 1107 68-69 1.1384588041992032 1107 70-71 1.155653821824707 1107 72-73 1.0024156432737321 1107 74-75 0.5640075381554226 1107 76-77 0.7567097578774558 1107 78-79 1.1609170079607587 1107 80-81 0.9997623525518584 1107 82-83 1.170621400541659 1107 84-85 1.044696281536396 1107 86-87 1.1885932813147129 1107 88-89 1.3014928497297902 1107 90-91 1.4077369813254883 1107 92-93 1.5202253614732903 1107 94-95 1.6038957567887415 1107 96-97 1.9452867122379338 1107 98-99 1.996138365707349 1107 100-101 1.5857044006594698 1108 1 0.0142245048760401 1108 2 -0.13424164801759275 1108 3 0.10020389381799788 1108 4 -0.024258957821231775 1108 5 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-0.0539652540771165 2316 100-101 0.4234772660919113 >>END_MODULE >>Per sequence quality scores pass #Quality Count 3 1.0 4 2.0 5 3.0 6 17.0 7 28.0 8 78.0 9 151.0 10 254.0 11 455.0 12 715.0 13 1135.0 14 1549.0 15 2267.0 16 3369.0 17 5216.0 18 4649.0 19 2443.0 20 1913.0 21 1842.0 22 1839.0 23 2017.0 24 2228.0 25 2542.0 26 3111.0 27 3554.0 28 4359.0 29 5187.0 30 6544.0 31 8188.0 32 10530.0 33 14399.0 34 20422.0 35 30496.0 36 50005.0 37 82735.0 38 73277.0 39 6892.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.14442052031685 20.626141138556445 15.302756228817504 20.9266821123092 2 15.126745144069615 21.443687008340575 40.9588275792016 22.470740268388205 3 19.598377030123135 26.005327133392775 30.64512488290464 23.75117095357945 4 12.484340259359165 17.883705969323838 38.13894563389502 31.493008137421985 5 12.732624757991228 38.85912972799061 34.01302755089945 14.395217963118709 6 30.431531663713418 37.828572395968536 17.358610882250037 14.381285058068011 7 27.4906041556155 34.71355371714276 20.601446903603716 17.194395223638026 8 26.265758495761993 35.38395991106396 19.509497421080553 18.840784172093493 9 26.742886809701705 17.618195772152184 21.02750471203007 34.61141270611605 10-11 24.435261785718314 28.164960554383033 27.031110684739794 20.368666975158856 12-13 25.992155866875464 25.591625400318847 28.153524922052455 20.262693810753234 14-15 22.347437445684683 26.80425606356444 27.496952699118538 23.351353791632338 16-17 20.51299053079467 30.26675169012336 27.97563287924788 21.24462489983409 18-19 20.279364129882733 28.23183187928174 31.751887633601573 19.73691635723395 20-21 21.46400234755031 27.14312720788235 31.642692685349257 19.750177759218086 22-23 20.99131369591869 27.485514596856454 31.47370901092948 20.049462696295375 24-25 20.921413496157015 27.35714366330711 31.969148900150106 19.752293940385766 26-27 20.57230568679927 27.674703061580068 31.958178203965197 19.79481304765546 28-29 20.084534980016056 28.03945718159361 32.179583222233035 19.696424616157298 30-31 20.446401363384986 27.631965057616558 31.832584675462456 20.089048903535996 32-33 20.308708508741237 27.9653341308985 31.575539202961522 20.150418157398732 34-35 20.64193650327867 27.695450492646977 31.822003769624054 19.8406092344503 36-37 21.25619335688408 27.368006726634537 31.570601446903602 19.80519846957778 38-39 20.560957303928763 27.828910984955364 31.736086814216225 19.874044896899655 40-41 20.56917290475129 28.15659308877596 31.473664607840096 19.800569398632653 42-43 20.542461748587616 28.210099163012252 30.966571285055565 20.280867803344567 44-45 20.91830976377775 28.0731182917057 30.632145638409536 20.376426306107017 46-47 20.838459194383937 28.831980858435948 30.144013182397888 20.18554676478223 48-49 21.206674717560354 28.991540918479057 29.67238693949415 20.12939742446644 50-51 21.06164576820198 29.023001478505243 29.458088326580363 20.457264426712413 52-53 21.153488030879316 29.477416114578514 28.935391578163266 20.433704276378904 54-55 20.73660034084625 29.33436226764331 29.180304278636164 20.74873311287428 56-57 21.202160197735967 29.42042030179565 29.115408056160625 20.262011444307753 58-59 21.25929708926334 28.80122569213232 29.095798110673453 20.843679107930882 60-61 20.94398609527894 28.91493516020902 29.902345293048764 20.23873345146327 62-63 20.922118889879577 28.748885477918357 29.92421249844813 20.404783133753938 64-65 20.73547171089015 28.984063745019924 29.74433709919529 20.536127444894642 66-67 20.531189688836722 28.929607349638275 29.510287462049817 21.028915499475186 68-69 20.456135796756318 28.882769206460278 29.64967326162771 21.011421735155693 70-71 20.66493233863413 29.237864406397073 29.205275216414794 20.891928038554 72-73 20.60723113212871 28.56816360619844 29.53652810852906 21.2880771531438 74-75 20.53048429511416 28.94667787772423 29.031466203175967 21.491371623985643 76-77 20.391662810514315 28.38969899439071 29.58900940148753 21.629628793607438 78-79 20.578592146992765 28.18964933467264 29.07181472410641 22.159943794228187 80-81 20.494227057774566 28.462213469069898 29.32349920431588 21.720060268839656 82-83 20.453545066702784 28.61461930881921 29.34033656125144 21.591499063226564 84-85 20.104567565432323 28.86978996196517 28.82605555116644 22.19958692143607 86-87 20.015264720156203 28.647167703125177 28.602304662370347 22.735262914348272 88-89 20.027538570928748 28.315350496032863 28.906611514282808 22.750499418755574 90-91 19.94276990283865 27.74220493905898 29.2700340615026 23.044991096599777 92-93 20.165206472820643 27.58553067817892 29.087343575852486 23.16191927314795 94-95 19.30719050145029 28.376437592406578 29.050935069918626 23.265436836224506 96-97 19.2920950757875 28.582835795627688 29.396013678995068 22.729055449589744 98-99 19.309524615697164 29.63604094715695 27.98596532809643 23.06846910904946 100-101 19.03209692577321 30.379896876666862 27.07312048270977 23.514885714850156 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 161.0 1 115.0 2 75.5 3 85.0 4 86.0 5 105.0 6 175.0 7 322.0 8 512.5 9 622.0 10 624.5 11 659.5 12 752.0 13 829.5 14 894.5 15 946.5 16 990.0 17 1026.5 18 1051.5 19 1102.0 20 1194.5 21 1282.5 22 1438.0 23 1720.0 24 1961.0 25 2242.0 26 2784.0 27 3411.0 28 3951.0 29 4566.5 30 5292.0 31 6064.0 32 6842.5 33 7568.0 34 8540.5 35 9438.0 36 10192.0 37 10945.5 38 11562.0 39 11877.5 40 12129.0 41 12713.5 42 13119.5 43 13504.5 44 14070.0 45 14142.0 46 13923.0 47 14547.0 48 15693.5 49 14607.0 50 12444.5 51 11026.0 52 9848.5 53 9248.0 54 8550.0 55 7548.5 56 6515.0 57 5574.5 58 4879.5 59 4255.5 60 3363.0 61 2514.0 62 1839.5 63 1295.5 64 893.0 65 615.0 66 467.0 67 363.5 68 252.0 69 174.0 70 104.0 71 60.0 72 49.0 73 49.0 74 37.0 75 19.5 76 10.0 77 7.0 78 9.0 79 6.0 80 5.0 81 3.5 82 2.0 83 2.0 84 2.0 85 1.5 86 1.5 87 1.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.014390031940227758 2 0.0 3 0.0 4 0.0 5 0.024265544056070336 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0019751024231685157 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 9.875512115842579E-4 24-25 0.0 26-27 9.875512115842579E-4 28-29 7.053937225601841E-4 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 8.46472467072221E-4 42-43 0.012979244495107388 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 0.0011286299560962947 84-85 0.0 86-87 0.0 88-89 0.0 90-91 0.014954346918275905 92-93 0.0019751024231685157 94-95 0.0 96-97 0.0 98-99 0.0011286299560962947 100-101 0.02229044163290182 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 354412.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 68.1359802184878 #Duplication Level Percentage of deduplicated Percentage of total 1 85.75829571841867 58.432255406414015 2 8.622243621688988 11.74970041692767 3 2.3101387710087904 4.7221070881025 4 1.0409188615110434 2.836961078278692 5 0.5659247222633359 1.927991784064393 6 0.3615309192979649 1.4779958139394707 7 0.25265353072498054 1.2050357180125864 8 0.17329505135126072 0.9446102552665062 9 0.12346149612573254 0.7570953051991041 >10 0.72163094103171 8.86448039554702 >50 0.04369147911113008 1.973016844092477 >100 0.02413434084210338 2.8465424794562306 >500 4.1610932486385143E-4 0.16614281802403894 >1k 0.0016644372994554057 2.0960645966752898 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2683 0.7570285430515897 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2051 0.578705009988375 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1609 0.4539913998397345 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 1050 0.2962653634752774 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 586 0.16534428856810718 No Hit GCTAAGGGTAGTTTATTTTGGACACAGACATATTAGGGCATTGTCACCAC 439 0.12386713768156835 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 395 0.1114522081645091 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 373 0.10524474340597947 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 1.4107874451203684E-4 0.0 18-19 0.0 0.0 0.0 2.821574890240737E-4 0.0 20-21 0.0 0.0 0.0 5.643149780481474E-4 0.0 22-23 0.0 0.0 0.0 0.0014107874451203682 0.0 24-25 0.0 0.0 0.0 0.002539417401216663 0.0 26-27 0.0 0.0 0.0 0.0029626536347527738 0.0 28-29 0.0 0.0 0.0 0.00578422852499351 0.0 30-31 0.0 0.0 0.0 0.012838165750595352 0.0 32-33 0.0 0.0 0.0 0.02102073293229349 0.0 34-35 0.0 0.0 0.0 0.02849790639143144 0.0 36-37 0.0 0.0 0.0 0.04077175716397864 0.0 38-39 0.0 0.0 0.0 0.057983363994447146 0.0 40-41 0.0 0.0 0.0 0.07547712831393971 0.0 42-43 0.0 0.0 0.0 0.11780075166755076 0.0 44-45 0.0 0.0 0.0 0.16760154848029976 0.0 46-47 2.821574890240737E-4 0.0 0.0 0.21627371533695247 0.0 48-49 2.821574890240737E-4 0.0 0.0 0.2772197329661524 0.0 50-51 2.821574890240737E-4 0.0 0.0 0.3495931289008273 0.0 52-53 2.821574890240737E-4 0.0 0.0 0.4997009130616345 0.0 54-55 2.821574890240737E-4 0.0 0.0 0.6348543503041658 0.0 56-57 2.821574890240737E-4 0.0 0.0 0.8701736961502433 0.0 58-59 2.821574890240737E-4 0.0 0.0 1.164746114691376 0.0 60-61 2.821574890240737E-4 0.0 0.0 1.4809035811428508 0.0 62-63 2.821574890240737E-4 0.0 0.0 1.9298161461801517 0.0 64-65 2.821574890240737E-4 2.821574890240737E-4 0.0 2.2018159655993585 0.0 66-67 2.821574890240737E-4 2.821574890240737E-4 0.0 2.6567949166506777 0.0 68-69 2.821574890240737E-4 2.821574890240737E-4 0.0 3.14803110504159 0.0 70-71 2.821574890240737E-4 2.821574890240737E-4 0.0 3.645615836935544 0.0 72-73 2.821574890240737E-4 2.821574890240737E-4 0.0 4.49110075279618 0.0 74-75 2.821574890240737E-4 2.821574890240737E-4 0.0 5.279448777129442 0.0 76-77 2.821574890240737E-4 2.821574890240737E-4 0.0 5.873813527758654 0.0 78-79 2.821574890240737E-4 2.821574890240737E-4 0.0 6.515016421565861 0.0 80-81 2.821574890240737E-4 2.821574890240737E-4 0.0 7.588484588557949 0.0 82-83 2.821574890240737E-4 2.821574890240737E-4 0.0 8.290633499994357 0.0 84-85 2.821574890240737E-4 2.821574890240737E-4 0.0 9.122998092615376 0.0 86-87 2.821574890240737E-4 2.821574890240737E-4 0.0 10.637196257463067 0.0 88-89 2.821574890240737E-4 2.821574890240737E-4 0.0 12.565037301220048 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTACACG 35 2.780344E-4 54.305313 3 TGTTACG 55 0.0025726694 34.557926 2 CCTAGAC 100 1.292181E-6 33.262005 3 AACCGAC 30 0.0039511444 31.664696 76-77 TATTCGT 80 4.4779602E-4 29.69822 4 GTATCAA 3385 0.0 28.777002 1 GTATAGT 100 4.9314218E-5 28.51029 1 ACTATAC 70 0.008369125 27.152657 3 GTGTAGC 165 8.346615E-8 25.918444 1 GGTATCA 2390 0.0 25.448515 1 GAGTACT 2165 0.0 24.900295 12-13 GTACATG 1880 0.0 24.516827 1 TATCAAC 3965 0.0 24.327822 2 ATCAACG 3975 0.0 24.147081 3 TCAACGC 3975 0.0 24.147081 4 CAACGCA 4010 0.0 23.936317 5 GTACTTT 2265 0.0 23.800943 14-15 AGTACTT 2240 0.0 23.74852 12-13 TACATGG 1985 0.0 23.459349 2 AACGCAG 4115 0.0 23.431103 6 >>END_MODULE