FastQCFastQC Report
Thu 26 May 2016
SRR937690_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937690_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences348763
Sequences flagged as poor quality0
Sequence length101
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT27020.7747381459615842No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT18630.5341736365382795No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12620.3618503109561507No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5090.1459443805678928No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT4480.12845399311280153No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT4150.11899198022726033No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT4140.11870525256406214No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN3950.11325742696329599No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC3570.10236177576176371No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTGGTCG550.002575445234.5501569
GTATCAA25750.032.484441
GGTATCA16600.031.4937611
ACTGTGC2002.3646862E-1128.503888
GTAGACC2350.028.3098583
TATCAAC30200.027.5364932
AGTCACG700.00835464427.162165
CACGGTG700.00837811127.146559
ATCAACG31250.026.4592043
TCAACGC31400.026.3441644
CAACGCA31650.026.1323185
AACGCAG32250.025.7787026
GTACATG15150.025.410451
CAATGCA1551.4042544E-624.5194669
GAGTACT19700.024.35610812-13
TACATGG15600.023.4554442
GTACTTT20250.023.22538414-15
ACGCAGA36200.022.83467
ATGGGGC1252.2407004E-422.8162165
TCTAGCA1252.245068E-422.8096582
Produced by FastQC (version 0.11.5)