##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937690_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 348763 Sequences flagged as poor quality 0 Sequence length 101 %GC 42 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.97436367963345 33.0 31.0 34.0 30.0 34.0 2 32.092171474611696 33.0 31.0 34.0 30.0 34.0 3 32.131232384169195 34.0 31.0 34.0 30.0 34.0 4 35.68833849921007 37.0 35.0 37.0 35.0 37.0 5 35.678108056187156 37.0 35.0 37.0 35.0 37.0 6 35.77432525812658 37.0 35.0 37.0 35.0 37.0 7 35.73264939228072 37.0 35.0 37.0 35.0 37.0 8 35.7258969558124 37.0 35.0 37.0 35.0 37.0 9 37.38196999108277 39.0 37.0 39.0 35.0 39.0 10-11 37.28328406396321 39.0 37.0 39.0 35.0 39.0 12-13 37.17415981626492 39.0 37.0 39.0 34.0 39.0 14-15 38.35676949676428 40.0 38.0 41.0 34.0 41.0 16-17 38.32825729793585 40.0 38.0 41.0 34.0 41.0 18-19 38.31858740749449 40.0 38.0 41.0 34.0 41.0 20-21 38.07159159658565 40.0 38.0 41.0 33.5 41.0 22-23 38.0369548948713 40.0 38.0 41.0 33.5 41.0 24-25 37.908379042501636 40.0 38.0 41.0 33.0 41.0 26-27 37.99643740878476 40.0 38.0 41.0 33.5 41.0 28-29 37.93051585173886 40.0 38.0 41.0 33.0 41.0 30-31 37.699591126352274 40.0 37.5 41.0 33.0 41.0 32-33 37.442113985715224 40.0 37.0 41.0 33.0 41.0 34-35 37.167778692120436 39.5 36.0 41.0 32.0 41.0 36-37 37.199249920433076 40.0 37.0 41.0 32.0 41.0 38-39 37.14284055361377 40.0 36.5 41.0 32.0 41.0 40-41 36.93929115187104 40.0 36.0 41.0 31.5 41.0 42-43 36.659513767228745 40.0 36.0 41.0 31.0 41.0 44-45 36.36772249349845 40.0 36.0 41.0 30.0 41.0 46-47 35.91619237132379 39.0 35.5 41.0 29.0 41.0 48-49 35.49089066214019 39.0 35.0 41.0 26.5 41.0 50-51 34.59438787944822 38.5 34.0 40.0 25.0 40.5 52-53 34.67451535856728 38.5 35.0 40.0 24.5 40.5 54-55 34.96404148375831 39.0 35.0 40.0 24.0 41.0 56-57 34.730884583513735 39.0 35.0 40.0 23.5 41.0 58-59 34.51972686322804 38.5 34.5 40.0 23.0 41.0 60-61 34.22230712546916 38.0 34.0 40.0 22.0 41.0 62-63 33.87110874720082 38.0 34.0 40.0 19.5 41.0 64-65 33.50671659551042 37.5 34.0 40.0 18.0 41.0 66-67 33.1016119829225 37.0 33.0 40.0 13.5 41.0 68-69 32.6433638315991 36.5 32.5 39.0 8.0 41.0 70-71 32.191245917714895 36.0 32.0 39.0 6.0 40.5 72-73 31.718847756212675 35.5 31.5 39.0 2.0 40.0 74-75 31.229546998964913 35.0 31.0 38.0 2.0 40.0 76-77 30.779258407571902 35.0 31.0 37.0 2.0 39.0 78-79 30.2585480684591 35.0 30.0 37.0 2.0 39.0 80-81 29.72365761276282 34.0 29.5 36.0 2.0 38.5 82-83 29.205509185320693 34.0 29.0 36.0 2.0 37.0 84-85 28.74102040640779 34.0 29.0 35.0 2.0 37.0 86-87 28.258486422011508 34.0 28.5 35.0 2.0 36.5 88-89 27.79814515874677 34.0 27.0 35.0 2.0 36.0 90-91 27.307720429059273 33.5 26.0 35.0 2.0 36.0 92-93 26.785850276548828 33.0 25.5 35.0 2.0 35.0 94-95 26.21086669170755 33.0 24.5 35.0 2.0 35.0 96-97 25.52871720910762 32.0 21.5 35.0 2.0 35.0 98-99 24.666763676192716 32.0 10.0 34.0 2.0 35.0 100-101 22.184641145993126 29.0 2.0 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.4373855478099564 1101 2 0.4881832228451479 1101 3 0.4816620722040028 1101 4 0.36874393775342185 1101 5 0.3855316575455561 1101 6 0.3598421057397658 1101 7 0.47919033329258554 1101 8 0.3201651943696291 1101 9 0.5262785809996302 1101 10-11 0.5094213729304684 1101 12-13 0.4567921585733714 1101 14-15 0.8895972992785275 1101 16-17 0.7712504281126158 1101 18-19 0.7532472835385136 1101 20-21 0.7077641267159223 1101 22-23 0.8037360219028713 1101 24-25 0.7022827988163627 1101 26-27 0.7801886023126983 1101 28-29 0.7632664625487493 1101 30-31 0.7841294308481395 1101 32-33 0.8633637402706285 1101 34-35 0.7579442511764256 1101 36-37 0.8315898548842284 1101 38-39 0.9442204062663322 1101 40-41 1.2105091866500999 1101 42-43 1.2257880255961808 1101 44-45 1.5808517106482327 1101 46-47 1.7709291017324276 1101 48-49 1.8190232508891526 1101 50-51 2.03108037673978 1101 52-53 1.9554423878405913 1101 54-55 2.1384361960473868 1101 56-57 2.2434825818573643 1101 58-59 2.1014981454177573 1101 60-61 2.2569846682638257 1101 62-63 2.2393265046156117 1101 64-65 2.1984071248860886 1101 66-67 2.2780821845650507 1101 68-69 2.5577844102391154 1101 70-71 2.643266222314299 1101 72-73 2.4024931519260733 1101 74-75 2.660151410163081 1101 76-77 2.631333940127366 1101 78-79 2.491966453187569 1101 80-81 2.3885805248302496 1101 82-83 2.5894304903394563 1101 84-85 2.550644977703243 1101 86-87 2.438001449507201 1101 88-89 2.6724076074145664 1101 90-91 2.5607409316821474 1101 92-93 2.5490654890115216 1101 94-95 2.5224909206836443 1101 96-97 2.3696213143271336 1101 98-99 1.9823283272915084 1101 100-101 2.4114150002467234 1102 1 -0.02244147515614614 1102 2 0.20444916040961303 1102 3 0.192893879287503 1102 4 -0.09779525918730769 1102 5 0.03391842655747723 1102 6 0.13703179855004066 1102 7 0.16107331362098876 1102 8 0.19246146603409642 1102 9 0.08390936832532248 1102 10-11 -0.03190930016510407 1102 12-13 0.19202152710558806 1102 14-15 0.28764461086810655 1102 16-17 0.2490617667602706 1102 18-19 0.2030845595043118 1102 20-21 0.13960477108462044 1102 22-23 0.2769412081349074 1102 24-25 0.46795095262982755 1102 26-27 0.6245939437252659 1102 28-29 0.5424190043997754 1102 30-31 0.36963113506295286 1102 32-33 0.13616896714519555 1102 34-35 0.1204986336410272 1102 36-37 0.5214027453372339 1102 38-39 0.5374734398302863 1102 40-41 0.848290412884225 1102 42-43 1.1018021642672338 1102 44-45 1.200397684421489 1102 46-47 1.3560428546074235 1102 48-49 1.6316581486516526 1102 50-51 1.4790902787358817 1102 52-53 1.3065383503755754 1102 54-55 1.3420598463000175 1102 56-57 1.36671563284834 1102 58-59 1.4449567008224378 1102 60-61 1.2757110178572475 1102 62-63 1.3860968097116384 1102 64-65 1.6135612431743027 1102 66-67 1.6768145689570915 1102 68-69 1.3292826105759517 1102 70-71 1.6570323369200501 1102 72-73 1.5113797007910108 1102 74-75 1.5390644074566637 1102 76-77 1.5234265065486348 1102 78-79 1.6602141755129303 1102 80-81 1.5968934599284559 1102 82-83 1.6082368933839497 1102 84-85 1.815905166814126 1102 86-87 1.5368751590301741 1102 88-89 1.6811811577543345 1102 90-91 1.9590314826038409 1102 92-93 1.890906041010858 1102 94-95 1.7454413214127484 1102 96-97 1.3863032219392935 1102 98-99 1.7626431836725374 1102 100-101 1.6069409360253815 1103 1 -0.13646893908800806 1103 2 0.24439571003589222 1103 3 0.3273563336591323 1103 4 -0.05739242588294502 1103 5 -0.16616739822958948 1103 6 0.1432513796381727 1103 7 0.18559312936929473 1103 8 -0.04678327583970798 1103 9 0.17784405457597785 1103 10-11 0.1801023676603748 1103 12-13 0.26909544506143845 1103 14-15 0.38516438316082 1103 16-17 0.36632938800135406 1103 18-19 0.15557673749840717 1103 20-21 0.26051994577608895 1103 22-23 0.2979329733009095 1103 24-25 0.23337497053353218 1103 26-27 0.1318048554235176 1103 28-29 0.05292710886701002 1103 30-31 -0.07975396836135218 1103 32-33 0.03430092003933538 1103 34-35 0.23072916553742573 1103 36-37 0.5733050137189508 1103 38-39 0.3695012948624381 1103 40-41 0.0513610933987394 1103 42-43 0.39561354528583337 1103 44-45 0.6583408227840835 1103 46-47 0.7522573296639123 1103 48-49 0.8782523169116914 1103 50-51 0.48218105746002493 1103 52-53 0.6028402798036936 1103 54-55 0.7000578473429542 1103 56-57 1.0759352791198467 1103 58-59 0.8616632017784625 1103 60-61 0.9841864871775954 1103 62-63 0.8577800619278619 1103 64-65 0.9030623730195941 1103 66-67 0.7360472519051129 1103 68-69 0.6939238846938025 1103 70-71 0.9399879687643029 1103 72-73 1.054436040961349 1103 74-75 0.7311055164432787 1103 76-77 0.7372687410349918 1103 78-79 0.7942991094604359 1103 80-81 0.9226256865928022 1103 82-83 0.9232029980250083 1103 84-85 1.2692691725502634 1103 86-87 1.0767347974796806 1103 88-89 0.88358642457165 1103 90-91 1.1594568041751998 1103 92-93 1.414369342766463 1103 94-95 1.3982366021358494 1103 96-97 1.5326236199924814 1103 98-99 2.002306185584583 1103 100-101 2.0159911055021773 1104 1 0.5492137992263117 1104 2 0.5248290924429604 1104 3 0.41318169251080406 1104 4 0.3404038567817622 1104 5 0.20647476224151973 1104 6 0.3059653104442077 1104 7 0.4029974355893913 1104 8 0.4375284650509812 1104 9 0.5657604145318089 1104 10-11 0.5899236319224244 1104 12-13 0.4350758462392079 1104 14-15 0.5407932937239437 1104 16-17 0.2096480758924102 1104 18-19 0.45832333300815264 1104 20-21 0.4467087569649024 1104 22-23 0.6752505570329745 1104 24-25 0.3795207655280066 1104 26-27 0.23120791946472963 1104 28-29 0.20005054464176197 1104 30-31 0.30322818106817095 1104 32-33 0.5042068364913064 1104 34-35 0.3576039355043008 1104 36-37 0.27353734717497247 1104 38-39 0.8283598704973301 1104 40-41 0.9518660161076156 1104 42-43 0.6256524208839167 1104 44-45 0.7642538242562935 1104 46-47 1.4369796796823167 1104 48-49 1.220990171162704 1104 50-51 1.522885418888066 1104 52-53 1.267682017013854 1104 54-55 1.3462438981342686 1104 56-57 1.3386503600915063 1104 58-59 1.3912684649363527 1104 60-61 1.2779675416494456 1104 62-63 1.689775369252125 1104 64-65 1.8439898665786671 1104 66-67 1.6214620016290766 1104 68-69 1.8989639104575176 1104 70-71 1.582896047527644 1104 72-73 1.667238534520429 1104 74-75 1.169617662192266 1104 76-77 1.5150854966477993 1104 78-79 1.4809192327581933 1104 80-81 1.8739736807039549 1104 82-83 1.7135968147346183 1104 84-85 1.8439781806474294 1104 86-87 1.4905873922533246 1104 88-89 1.462464783055271 1104 90-91 1.4366984769760798 1104 92-93 1.602841232718614 1104 94-95 1.1951978645576382 1104 96-97 0.475793936518059 1104 98-99 1.0722707604833666 1104 100-101 1.0064849866210572 1105 1 0.3862583336392653 1105 2 0.2728048009449857 1105 3 0.2657139665922372 1105 4 0.36874393775342185 1105 5 0.5888175931940793 1105 6 0.5768384293808779 1105 7 0.567346988717496 1105 8 0.5027450260470872 1105 9 0.557185821299818 1105 10-11 0.5305794259794112 1105 12-13 0.7211216028658995 1105 14-15 0.8376193574318407 1105 16-17 0.5561074754799193 1105 18-19 0.7579566688877719 1105 20-21 0.5167377845410996 1105 22-23 0.44933198702303656 1105 24-25 0.6868215828834465 1105 26-27 0.9002171624561015 1105 28-29 0.9921691757623776 1105 30-31 0.8155076096832516 1105 32-33 0.9340918720118907 1105 34-35 0.4278239339765406 1105 36-37 0.5753954939933905 1105 38-39 0.5170677614147579 1105 40-41 0.7953844638961698 1105 42-43 0.38818966020864565 1105 44-45 0.9242874351339978 1105 46-47 1.426008401705083 1105 48-49 0.8517192980437684 1105 50-51 1.0375519837035938 1105 52-53 1.399795682891174 1105 54-55 1.0442902658678221 1105 56-57 1.0650272602519237 1105 58-59 1.2024650885709107 1105 60-61 1.3713809554451757 1105 62-63 1.245376545632844 1105 64-65 1.7877107435170245 1105 66-67 1.802540605249888 1105 68-69 1.5721936102512686 1105 70-71 1.4866753615601915 1105 72-73 1.7312424303267022 1105 74-75 1.6285111768206377 1105 76-77 1.5815815626473402 1105 78-79 1.7351492381053788 1105 80-81 1.825820369268616 1105 82-83 1.8931858453663502 1105 84-85 2.23831398387102 1105 86-87 1.528600149109181 1105 88-89 1.2914980883795373 1105 90-91 1.1451718556331727 1105 92-93 1.3030646686669805 1105 94-95 1.888641833696738 1105 96-97 2.210850463903288 1105 98-99 2.077188261056282 1105 100-101 1.4233935497560388 1106 1 -0.07183415190986864 1106 2 0.31182214198544855 1106 3 0.28114640197232177 1106 4 0.1837728394875242 1106 5 0.13895937528591418 1106 6 0.07994672908640865 1106 7 -0.10050119538109925 1106 8 0.22417048138572682 1106 9 0.4143948953536949 1106 10-11 0.14756328463356994 1106 12-13 0.16802148289644236 1106 14-15 -0.01773564311347542 1106 16-17 0.18714684727618902 1106 18-19 0.2226747406513141 1106 20-21 0.6076691838212795 1106 22-23 0.46563692758362407 1106 24-25 0.5802532548214998 1106 26-27 0.4453147739731733 1106 28-29 0.6702829286252836 1106 30-31 0.423234733688048 1106 32-33 0.726013019356202 1106 34-35 0.7093977151958626 1106 36-37 0.8924556968508526 1106 38-39 0.5851640641740588 1106 40-41 0.7327276856215548 1106 42-43 0.9990932483861954 1106 44-45 0.17971332672522777 1106 46-47 0.8931122298215612 1106 48-49 1.1540832417671822 1106 50-51 1.0788869166297559 1106 52-53 0.793131924575107 1106 54-55 0.4628337275321215 1106 56-57 0.9721599901025115 1106 58-59 1.158989791373834 1106 60-61 0.43898647404044766 1106 62-63 -0.15097256550250648 1106 64-65 -0.053847768861643885 1106 66-67 0.1840980269970771 1106 68-69 0.1695052036638529 1106 70-71 0.48338983423986903 1106 72-73 0.5699789337621937 1106 74-75 0.12380782858200945 1106 76-77 0.418750283336621 1106 78-79 0.08345603273947333 1106 80-81 -0.05665045633940835 1106 82-83 0.3646113007049898 1106 84-85 -0.13978068294106905 1106 86-87 -0.14932274324549155 1106 88-89 0.3465068134313434 1106 90-91 0.46354081626137855 1106 92-93 0.782792556113808 1106 94-95 0.7696403068126756 1106 96-97 0.649297949997738 1106 98-99 1.621345202925621 1106 100-101 1.1618873876881999 1107 1 -0.3806908189031084 1107 2 -0.6297375719363707 1107 3 -0.43159975262746286 1107 4 -0.5888831808906474 1107 5 -0.2731107726517763 1107 6 -0.24855331989957818 1107 7 -0.3360748213240825 1107 8 -0.17178327583970798 1107 9 -0.11862166191092882 1107 10-11 -0.32907039813315464 1107 12-13 -0.2638879909940286 1107 14-15 -0.2693521807837058 1107 16-17 -0.07340963357028585 1107 18-19 -0.3959448341502849 1107 20-21 0.09045060833386742 1107 22-23 -0.01988771236934639 1107 24-25 -0.12445919741715983 1107 26-27 -0.2622388656858803 1107 28-29 -0.3148743164026371 1107 30-31 0.23659811176192136 1107 32-33 0.10602183683440813 1107 34-35 -0.026445333692151962 1107 36-37 -0.6714465271130976 1107 38-39 -0.25631727216375566 1107 40-41 -0.24219148749493513 1107 42-43 -0.47849566118566145 1107 44-45 -0.295891650251356 1107 46-47 -0.17828485061343713 1107 48-49 -0.3754553102069522 1107 50-51 0.35039176624276536 1107 52-53 0.12653057256179068 1107 54-55 0.44495384117961834 1107 56-57 -0.09223209376150976 1107 58-59 0.03645133737168038 1107 60-61 0.4424154933409241 1107 62-63 0.32720417594943996 1107 64-65 0.3942025887360856 1107 66-67 0.5029723289467185 1107 68-69 0.6854011574210759 1107 70-71 0.3693504495039015 1107 72-73 0.10304447701682307 1107 74-75 0.028775007968704358 1107 76-77 -0.29737590457363794 1107 78-79 0.20432896308139448 1107 80-81 -0.08883425177391402 1107 82-83 -0.010156015842479604 1107 84-85 0.30131366407568905 1107 86-87 0.30622247082328613 1107 88-89 0.3085671641710306 1107 90-91 0.6239454405388329 1107 92-93 1.2703256216570615 1107 94-95 1.9424873725518736 1107 96-97 1.8199406461866268 1107 98-99 1.4117341516862787 1107 100-101 1.5263676463342257 1108 1 -0.2833220859411547 1108 2 -0.026146248006064354 1108 3 -0.12675205794926114 1108 4 -0.11377354476405799 1108 5 0.20205812624593733 1108 6 -0.3355510679142739 1108 7 -0.20907470279853868 1108 8 0.188356584300152 1108 9 0.4027129356948578 1108 10-11 0.17715219283520156 1108 12-13 0.38207621429220495 1108 14-15 0.43608221532866054 1108 16-17 0.24406103135800095 1108 18-19 0.21283198225364686 1108 20-21 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-0.07827800062990065 2316 52-53 -0.08664938215888895 2316 54-55 0.17119749289595632 2316 56-57 0.3067001708165975 2316 58-59 0.2693798082766605 2316 60-61 -0.26216750855591187 2316 62-63 0.3167868719048954 2316 64-65 0.3556386775814673 2316 66-67 0.4709555633590057 2316 68-69 0.5689751646412944 2316 70-71 0.4949087925253828 2316 72-73 0.4081292227795359 2316 74-75 0.2651759135551899 2316 76-77 0.514011233650681 2316 78-79 0.602435185929096 2316 80-81 0.6198483646367805 2316 82-83 0.4271412979921898 2316 84-85 0.6072091544997242 2316 86-87 0.8196991886841403 2316 88-89 0.7793650592943244 2316 90-91 0.9551728773619388 2316 92-93 0.9420964103739458 2316 94-95 1.0997565397794347 2316 96-97 0.8200477256701966 2316 98-99 0.6082403011080473 2316 100-101 1.0439745728471088 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1280.0 3 233.0 4 246.0 5 267.0 6 272.0 7 294.0 8 390.0 9 408.0 10 551.0 11 775.0 12 1182.0 13 1657.0 14 1935.0 15 2569.0 16 3588.0 17 5148.0 18 4223.0 19 2503.0 20 2339.0 21 2436.0 22 2634.0 23 2941.0 24 3093.0 25 3402.0 26 3824.0 27 4391.0 28 4995.0 29 5788.0 30 7183.0 31 9071.0 32 11625.0 33 15529.0 34 21500.0 35 31598.0 36 48072.0 37 69954.0 38 62153.0 39 8710.0 40 4.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.302688468791686 19.535669932385815 15.409824755071064 21.75181684375144 2 14.442097397651995 20.27819689327643 42.64143351870213 22.63827219036944 3 18.905078023967583 25.749058826910364 30.584245768313362 24.76161738080869 4 11.763032194650894 16.682473717186 39.12819805372098 32.426296034442124 5 12.439463919777658 37.69461361813912 35.29386404853808 14.572058413545136 6 29.802726442354643 37.0246965353616 18.494826882974387 14.67775013930937 7 26.681544157138465 33.949686510022204 21.853540009822588 17.515229323016744 8 25.151007723438294 34.919189685231835 20.82283771013985 19.106964881190024 9 25.8763173844479 17.3494038198045 21.850269868125114 34.92400892762249 10-11 23.75243796299754 27.319404031148476 28.24877419651346 20.67938380934052 12-13 25.027574004607157 25.04093016308875 29.139260212434724 20.792235619869366 14-15 21.74661739551303 25.855866077442645 28.5191509774043 23.87836554964002 16-17 19.947556690327424 29.631331574692226 28.9561167825771 21.464994952403245 18-19 19.90516086320273 27.48697828781179 32.51543433710976 20.092426511875715 20-21 20.857139985267278 26.422691263323706 32.447742633998125 20.272426117410888 22-23 20.3459467221943 26.6287068284627 32.60257054642062 20.42277590292238 24-25 20.15276888213618 26.689749061529756 32.85421327903082 20.303268777303238 26-27 19.979004690197115 26.805783331849398 32.76287937318517 20.45233260476832 28-29 19.696976224840768 27.129101053402554 32.986651156445 20.187271565311676 30-31 19.772115941113196 26.8376993877806 32.84643963470766 20.543745036398548 32-33 19.544152179064607 26.993257071457812 32.73357892242401 20.72901182705358 34-35 20.12827454083545 26.98102450170109 32.49415856812754 20.396542389335924 36-37 20.059737070729554 26.688685138249674 32.634534058028244 20.617043732992528 38-39 19.858853598299923 27.08460168858767 32.64846935845156 20.408075354660845 40-41 20.012032433006773 27.19502161673973 32.494174033779835 20.298771916473665 42-43 19.86677464841316 27.278457928760762 32.02864962507934 20.826117797746743 44-45 19.86375137497954 27.49111827839638 31.712987561281725 20.932142785342357 46-47 20.16692083896964 27.641884149125907 31.27195287616783 20.919242135736628 48-49 20.25393898398556 27.835917674480726 30.88700562537609 21.023137716157624 50-51 20.111756714450248 27.97565536078951 30.909448626664794 21.003139298095455 52-53 20.370043217669693 28.305203786608985 30.449282793507436 20.875470202213883 54-55 20.15120728896675 27.970476525535076 30.570437754435343 21.30787843106283 56-57 20.291523924660936 28.083299113539837 30.41718474955419 21.20799221224504 58-59 20.496893518099725 27.635986657994078 30.609938731877662 21.25718109202854 60-61 20.197788376774223 27.563309222371217 31.122667271594338 21.116235129260215 62-63 20.14759829716361 27.54388105989361 31.276337609568046 21.032183033374732 64-65 20.01002180964018 27.475264939029202 31.321744598919427 21.192968652411192 66-67 19.74331068624692 27.5061595097662 30.940552833948804 21.80997697003808 68-69 19.772882928026945 27.382841160195216 31.227233372913943 21.6170425388639 70-71 19.924878422791473 27.35993314868817 30.96927223100459 21.74591619751576 72-73 19.752035263543295 27.03935560756386 31.048142777147614 22.160466351745228 74-75 19.711653754747733 27.20334297838121 30.95773171449701 22.127271552374044 76-77 19.699445470287717 26.919962208125252 31.093868009867244 22.286724311719787 78-79 19.646000671987043 26.63595819895986 30.899917006728483 22.818124122324615 80-81 19.7083499054492 26.819977284834106 31.01625239966631 22.455420410050383 82-83 19.74723742827076 26.891376411793672 31.10375095292035 22.257635207015216 84-85 19.473947162140046 26.710511581431806 30.757911178874043 23.0576300775541 86-87 19.54377285406847 26.47217169462155 30.957347686202784 23.026707765107194 88-89 19.58616946894333 26.00783613321714 30.902045463355527 23.50394893448401 90-91 19.50861263186006 25.452240346773834 31.607935366135564 23.431211655230538 92-93 19.492027939899064 25.598748016855826 31.335329040409476 23.573895002835627 94-95 18.772738889351523 25.75786726088093 31.537098668473813 23.932295181293735 96-97 18.548234253585193 25.78523883091449 32.02163462919038 23.644892286309936 98-99 18.98539154507313 27.186170930696708 30.68421672319175 23.144220801038415 100-101 18.866957448091494 27.12738006701947 30.155603476742748 23.85005900814628 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 388.0 1 240.0 2 102.0 3 107.5 4 90.0 5 90.5 6 157.5 7 330.5 8 570.0 9 700.5 10 677.0 11 650.0 12 656.5 13 727.0 14 821.0 15 848.0 16 893.0 17 929.0 18 963.0 19 1038.5 20 1153.5 21 1293.5 22 1452.0 23 1693.5 24 1989.5 25 2389.0 26 2893.5 27 3468.0 28 4056.5 29 4590.0 30 5289.0 31 6035.5 32 6712.0 33 7563.5 34 8631.5 35 9466.5 36 10200.0 37 11089.0 38 11559.0 39 11712.0 40 12051.0 41 12499.0 42 12904.0 43 13420.5 44 13832.5 45 14095.5 46 14001.0 47 14146.0 48 15173.5 49 14540.0 50 12352.0 51 10802.5 52 9721.5 53 8949.5 54 8219.0 55 7210.5 56 6118.0 57 5213.0 58 4466.5 59 3711.5 60 2998.0 61 2329.5 62 1705.5 63 1234.0 64 875.5 65 626.0 66 442.0 67 326.0 68 236.0 69 146.0 70 92.0 71 67.5 72 56.0 73 42.5 74 32.0 75 27.0 76 22.5 77 15.0 78 8.0 79 6.0 80 5.0 81 3.5 82 3.5 83 2.5 84 1.5 85 2.0 86 1.5 87 1.5 88 1.5 89 1.0 90 0.0 91 1.5 92 1.5 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.2600619905207835 2 0.2316759518641599 3 0.22737503691618663 4 0.23425650083294386 5 0.23798396045452067 6 0.17691096819330032 7 0.16802241063415557 8 0.17261005324532708 9 0.18063842781487716 10-11 0.17891806183568784 12-13 0.17461714688771457 14-15 0.1782012426776923 16-17 0.16558522549697072 18-19 0.17045959577134043 20-21 0.16013739989620457 22-23 0.168595865960552 24-25 0.16916932128694845 26-27 0.16916932128694845 28-29 0.17361360006652082 30-31 0.17304014474012439 32-33 0.17791451501449407 34-35 0.17304014474012439 36-37 0.1635781318545832 38-39 0.15569312111663222 40-41 0.154116118969042 42-43 0.15942058073820906 44-45 0.16515513400217338 46-47 0.16458167867577697 48-49 0.17447378305611547 50-51 0.17088968726613776 52-53 0.18393579594165665 54-55 0.16214449353859212 56-57 0.15024529551586607 58-59 0.15468957429543845 60-61 0.14680456355748747 62-63 0.14924174869467233 64-65 0.14981520402106874 66-67 0.14938511252627143 68-69 0.15153557000025805 70-71 0.15124884233705982 72-73 0.15139220616865895 74-75 0.1635781318545832 76-77 0.15483293812703755 78-79 0.155406393453434 80-81 0.154116118969042 82-83 0.140926646461924 84-85 0.14049655496712668 86-87 0.14150010178832045 88-89 0.14465410608350082 90-91 0.15038865934746518 92-93 0.14780811037868122 94-95 0.14795147421028035 96-97 0.1505320231790643 98-99 0.15483293812703755 100-101 0.14508419757829816 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 348763.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 72.38236768585152 #Duplication Level Percentage of deduplicated Percentage of total 1 87.81189910460053 63.56033168182091 2 7.493687292843466 10.848216579067781 3 1.9294459903302894 4.189736073062368 4 0.8427703331508493 2.4400684851540944 5 0.4838569376374727 1.751135538371284 6 0.29973669201474124 1.3017390870211103 7 0.19442787516683452 0.9851204964093261 8 0.16171853391943875 0.9364456307018934 9 0.11423513826049117 0.7441748802193462 >10 0.6201189421027242 7.922752102279299 >50 0.03180374213124529 1.5417846974634104 >100 0.014709230735415925 1.9552868643344892 >500 3.975467766328628E-4 0.14646666871909567 >1k 0.0011926403298985885 1.6767412153755805 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2702 0.7747381459615842 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1863 0.5341736365382795 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1262 0.3618503109561507 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 509 0.1459443805678928 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 448 0.12845399311280153 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 415 0.11899198022726033 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 414 0.11870525256406214 No Hit NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 395 0.11325742696329599 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 357 0.10236177576176371 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 2.867276631982177E-4 0.0 24-25 0.0 0.0 0.0 5.734553263964354E-4 0.0 26-27 0.0 0.0 0.0 0.001863729810788415 0.0 28-29 0.0 0.0 0.0 0.005304461769167027 0.0 30-31 0.0 0.0 0.0 0.014479746991509994 0.0 32-33 0.0 0.0 0.0 0.024801942866645832 0.0 34-35 0.0 0.0 0.0 0.03541086640497988 0.0 36-37 0.0 0.0 0.0 0.04745342825930503 0.0 38-39 0.0 0.0 0.0 0.06408363272480166 0.0 40-41 0.0 0.0 0.0 0.08014038186390185 0.0 42-43 0.0 0.0 0.0 0.1247265334912247 0.0 44-45 0.0 0.0 0.0 0.1734702362349217 0.0 46-47 0.0 0.0 0.0 0.22164048365222228 0.0 48-49 0.0 0.0 0.0 0.2900250313249972 0.0 50-51 0.0 0.0 0.0 0.3733194174840795 0.0 52-53 0.0 0.0 0.0 0.5370409131702618 0.0 54-55 0.0 0.0 0.0 0.6692223659046401 0.0 56-57 0.0 0.0 0.0 0.9371693671633745 0.0 58-59 0.0 0.0 0.0 1.2786620140324518 0.0 60-61 0.0 0.0 0.0 1.6301901291134668 0.0 62-63 0.0 0.0 0.0 2.1550451165978046 0.0 64-65 0.0 0.0 0.0 2.472309275926632 0.0 66-67 0.0 0.0 0.0 2.9779535099766887 0.0 68-69 0.0 0.0 0.0 3.5660319471962336 0.0 70-71 0.0 0.0 0.0 4.117265879694807 0.0 72-73 0.0 0.0 0.0 5.018307561295206 0.0 74-75 0.0 0.0 0.0 5.8645842592247455 0.0 76-77 0.0 0.0 0.0 6.486926652196477 0.0 78-79 0.0 0.0 0.0 7.170055309766231 0.0 80-81 0.0 0.0 0.0 8.264638164025428 0.0 82-83 0.0 0.0 0.0 8.988768877432525 0.0 84-85 0.0 0.0 0.0 9.958051742874101 0.0 86-87 0.0 0.0 0.0 11.55397791623538 0.0 88-89 0.0 0.0 0.0 13.597629335680676 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TTGGTCG 55 0.0025754452 34.550156 9 GTATCAA 2575 0.0 32.48444 1 GGTATCA 1660 0.0 31.493761 1 ACTGTGC 200 2.3646862E-11 28.50388 8 GTAGACC 235 0.0 28.309858 3 TATCAAC 3020 0.0 27.536493 2 AGTCACG 70 0.008354644 27.16216 5 CACGGTG 70 0.008378111 27.14655 9 ATCAACG 3125 0.0 26.459204 3 TCAACGC 3140 0.0 26.344164 4 CAACGCA 3165 0.0 26.132318 5 AACGCAG 3225 0.0 25.778702 6 GTACATG 1515 0.0 25.41045 1 CAATGCA 155 1.4042544E-6 24.519466 9 GAGTACT 1970 0.0 24.356108 12-13 TACATGG 1560 0.0 23.455444 2 GTACTTT 2025 0.0 23.225384 14-15 ACGCAGA 3620 0.0 22.8346 7 ATGGGGC 125 2.2407004E-4 22.816216 5 TCTAGCA 125 2.245068E-4 22.809658 2 >>END_MODULE