FastQCFastQC Report
Thu 26 May 2016
SRR937690_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937690_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences348763
Sequences flagged as poor quality0
Sequence length101
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT26130.7492193839369429No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19820.5682942284588675No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14410.41317456266863173No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT11030.31626061250763415No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5240.15024529551586607No Hit
GCTAAGGGTAGTTTATTTTGGACACAGACATATTAGGGCATTGTCACCAC4430.12702035479681045No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3560.10207504809856549No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3500.1003546821193762No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAAGGG1600.047.5039442
TAAGGGT1700.044.709593
GGTAGTT1700.044.709597
TAGTTTA2100.038.4555749
AAGGGTA2000.038.0031554
GGGTAGT2400.035.622856
GCTAAGG2550.035.400171
AGGGTAG2150.035.3517725
GTAGTTT2450.031.0229848
CTAGTAG650.00583619529.2331963
TACCTGG1703.619789E-927.9434952
ACCGTGG856.3950574E-427.9434958
TAGTCGC1056.8883804E-527.1451117
GTCGCTC1109.452742E-525.9112429
AGTCGCT1151.2785317E-424.7846668
CTAGTCG1201.7081886E-423.7485666
GAGTACT20950.023.35516212-13
GTATCAA37600.023.1235871
CTAGTGA1252.24808E-422.8051621
GTACTTT21250.022.79862414-15
Produced by FastQC (version 0.11.5)