##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937690_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 348763 Sequences flagged as poor quality 0 Sequence length 101 %GC 42 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.414396022513856 33.0 31.0 34.0 31.0 34.0 2 32.62812855721507 34.0 31.0 34.0 31.0 34.0 3 32.70611848160499 34.0 31.0 34.0 31.0 34.0 4 36.26523455756488 37.0 35.0 37.0 35.0 37.0 5 36.20536295421246 37.0 35.0 37.0 35.0 37.0 6 36.253696636397784 37.0 37.0 37.0 35.0 37.0 7 36.20837072739941 37.0 37.0 37.0 35.0 37.0 8 36.1969302936378 37.0 36.0 37.0 35.0 37.0 9 37.89548489948762 39.0 38.0 39.0 35.0 39.0 10-11 37.841263264738515 39.0 38.0 39.0 35.0 39.0 12-13 37.76347261607452 39.0 37.5 39.0 35.0 39.0 14-15 38.99995842448884 40.0 38.0 41.0 36.0 41.0 16-17 38.982651542738196 40.0 38.0 41.0 36.0 41.0 18-19 39.00197842087607 40.0 38.0 41.0 36.0 41.0 20-21 38.92642281434671 40.0 38.0 41.0 35.0 41.0 22-23 38.77753087340113 40.0 38.0 41.0 35.0 41.0 24-25 38.83981242276273 40.0 38.0 41.0 35.0 41.0 26-27 38.71080504525996 40.0 38.0 41.0 35.0 41.0 28-29 38.564490786006544 40.0 38.0 41.0 34.0 41.0 30-31 38.58908198404074 40.0 38.0 41.0 35.0 41.0 32-33 38.48549731479544 40.0 38.0 41.0 35.0 41.0 34-35 38.34278292135347 40.0 38.0 41.0 34.0 41.0 36-37 38.18460243775859 40.0 38.0 41.0 34.0 41.0 38-39 38.02604490728661 40.0 38.0 41.0 34.0 41.0 40-41 37.84114570639661 40.0 37.0 41.0 33.0 41.0 42-43 37.59624874198238 40.0 37.0 41.0 33.0 41.0 44-45 37.20444112477527 40.0 37.0 41.0 32.5 41.0 46-47 36.74927242855463 40.0 37.0 41.0 31.0 41.0 48-49 36.32291986248541 40.0 36.0 41.0 30.0 41.0 50-51 36.02457399437441 40.0 36.0 41.0 29.5 41.0 52-53 35.78801937132093 39.0 36.0 41.0 28.5 41.0 54-55 35.56927913798195 39.0 35.0 41.0 28.0 41.0 56-57 35.300170889687266 39.0 35.0 40.0 27.0 41.0 58-59 35.04331021352609 39.0 35.0 40.0 26.5 41.0 60-61 34.686652540550455 38.0 34.5 40.0 26.0 41.0 62-63 34.36180586816836 38.0 34.0 40.0 25.5 41.0 64-65 33.97086846941906 37.5 34.0 40.0 23.5 41.0 66-67 33.556026585388935 37.0 33.5 39.5 22.5 41.0 68-69 33.10041202765201 36.0 33.0 39.0 21.5 40.5 70-71 32.647671914738666 36.0 32.5 39.0 19.5 40.0 72-73 32.15269968431284 35.0 32.0 38.0 17.5 40.0 74-75 31.65901916200973 35.0 31.5 37.5 12.5 39.5 76-77 30.41827544779693 34.0 30.0 36.0 10.5 39.0 78-79 30.957220232650826 35.0 31.0 36.0 7.5 39.0 80-81 30.724289847260174 35.0 31.0 36.0 6.0 38.5 82-83 30.364043777579617 34.0 31.0 36.0 2.0 37.0 84-85 29.99523315259933 34.0 31.0 35.0 2.0 37.0 86-87 29.579427863620854 34.0 30.0 35.0 2.0 36.0 88-89 29.244613104027664 34.0 30.0 35.0 2.0 36.0 90-91 28.794209821569375 34.0 29.0 35.0 2.0 35.5 92-93 28.36300295616221 34.0 29.0 35.0 2.0 35.0 94-95 27.94662564549565 34.0 29.0 35.0 2.0 35.0 96-97 27.47472065557413 34.0 27.5 35.0 2.0 35.0 98-99 26.693099612057473 33.0 25.5 35.0 2.0 35.0 100-101 24.191865249467405 31.0 13.0 33.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.28424430952473045 1101 2 0.2872372652131432 1101 3 0.13619421166297485 1101 4 0.1019248490544058 1101 5 0.24650168146357032 1101 6 0.21700369484862847 1101 7 0.2847120197311952 1101 8 0.24346576848687818 1101 9 0.48139023170155326 1101 10-11 0.24413672009982434 1101 12-13 0.31795456995786964 1101 14-15 0.42306577509294385 1101 16-17 0.39867725841390467 1101 18-19 0.3340832986140754 1101 20-21 0.304690851322988 1101 22-23 0.3302261091418117 1101 24-25 0.3114538804530085 1101 26-27 0.4022490690854639 1101 28-29 0.3676442577469672 1101 30-31 0.5103907022325274 1101 32-33 0.45055441351394165 1101 34-35 0.3814898122898356 1101 36-37 0.3550372499707706 1101 38-39 0.37745621869859747 1101 40-41 0.07085021609625386 1101 42-43 0.526766513198524 1101 44-45 0.7345050339615042 1101 46-47 0.5115090261123001 1101 48-49 0.5966446986313159 1101 50-51 0.6581493846260358 1101 52-53 0.400660972995766 1101 54-55 0.6755081297583487 1101 56-57 0.33487657012925354 1101 58-59 0.5608594372131819 1101 60-61 0.45111720399861355 1101 62-63 0.4686141266091113 1101 64-65 0.5565495767054998 1101 66-67 0.444113701518134 1101 68-69 0.43307786294951 1101 70-71 0.4832486672263201 1101 72-73 0.3665925231448064 1101 74-75 0.342076278922967 1101 76-77 0.28262669433098964 1101 78-79 0.39965130578533703 1101 80-81 0.5075938773853998 1101 82-83 0.5411269373824048 1101 84-85 0.6715171643615641 1101 86-87 0.3751642422976502 1101 88-89 0.6978514499411084 1101 90-91 0.22083884842190216 1101 92-93 0.44240572358459573 1101 94-95 0.6925185459567338 1101 96-97 0.6718791249822509 1101 98-99 0.6463797948944254 1101 100-101 1.174651389275926 1102 1 0.07798351385500979 1102 2 0.09635596908618993 1102 3 0.22578326035086604 1102 4 0.15180738600984967 1102 5 0.15870767746705639 1102 6 0.10566166032096191 1102 7 0.11882279523360495 1102 8 0.1331336390342841 1102 9 0.010192539704128478 1102 10-11 0.14762920585185668 1102 12-13 0.048657839525546365 1102 14-15 0.1542924710714928 1102 16-17 0.3773183818717243 1102 18-19 0.3802510475418188 1102 20-21 0.3282727506219061 1102 22-23 0.23331216968069413 1102 24-25 0.22233283655600644 1102 26-27 0.1038159614412777 1102 28-29 0.2846873295826171 1102 30-31 0.050899287965158635 1102 32-33 0.07435399662019648 1102 34-35 0.09346365328520534 1102 36-37 0.2541114010193226 1102 38-39 0.33290031521843133 1102 40-41 0.2421507459885177 1102 42-43 0.29521652443679613 1102 44-45 0.23519318574285109 1102 46-47 0.27698474374529525 1102 48-49 0.11741395071655347 1102 50-51 -0.0763656608043064 1102 52-53 0.31101034473012135 1102 54-55 0.25859129605707665 1102 56-57 0.06228071381904243 1102 58-59 0.2940597586585767 1102 60-61 0.11467564147840648 1102 62-63 0.27384561963676646 1102 64-65 0.3284003201249561 1102 66-67 0.49512622372526494 1102 68-69 0.5955417234348204 1102 70-71 0.4796093141813671 1102 72-73 0.5573860683734644 1102 74-75 0.31928105872979273 1102 76-77 0.06628994025211554 1102 78-79 0.511013353675775 1102 80-81 0.22528384268530388 1102 82-83 0.22000329482722236 1102 84-85 0.2196416043722742 1102 86-87 0.16628939355248917 1102 88-89 0.16397960320036375 1102 90-91 0.18444069950400888 1102 92-93 0.12878093410977343 1102 94-95 0.0706648775073404 1102 96-97 0.4157280949098663 1102 98-99 0.0813117340661158 1102 100-101 0.6023785178510046 1103 1 0.2616268303855165 1103 2 0.2952521971411173 1103 3 0.26041193234808446 1103 4 0.22219457530239595 1103 5 0.20396124082530775 1103 6 0.0754111388539016 1103 7 0.20996545993028803 1103 8 0.17139085266195764 1103 9 0.3064021260069296 1103 10-11 0.06856991070149121 1103 12-13 0.12742380945428522 1103 14-15 0.2065505320831349 1103 16-17 0.15268014964505738 1103 18-19 0.124311211803942 1103 20-21 -0.09350990625276978 1103 22-23 0.026523320384676197 1103 24-25 0.028140675944150928 1103 26-27 -0.13606431841270705 1103 28-29 0.08004681593756402 1103 30-31 0.1138094000805765 1103 32-33 0.08025037676969049 1103 34-35 0.14480914694068758 1103 36-37 0.05998985573859983 1103 38-39 0.0018637907963849898 1103 40-41 0.09387484645784383 1103 42-43 0.25089182428035883 1103 44-45 0.3453965578965068 1103 46-47 0.3903060543658441 1103 48-49 0.14121403328216786 1103 50-51 0.22106339794765262 1103 52-53 -0.013029829961340056 1103 54-55 0.33259073113443094 1103 56-57 0.1649092337216942 1103 58-59 0.523021821929909 1103 60-61 0.4003445316226859 1103 62-63 0.5070853423901127 1103 64-65 0.5836937539855427 1103 66-67 0.7646377829117768 1103 68-69 0.5765163236434105 1103 70-71 0.33320200495139574 1103 72-73 0.40279009670650723 1103 74-75 0.14131412843255475 1103 76-77 0.2687332307092696 1103 78-79 0.5710825567002829 1103 80-81 0.7027222443887631 1103 82-83 0.5170249574380357 1103 84-85 0.5062366417440849 1103 86-87 0.6309627161667777 1103 88-89 0.3959808416846293 1103 90-91 0.5026035058659062 1103 92-93 0.7284408941306317 1103 94-95 0.8610242552233167 1103 96-97 0.9340364774928638 1103 98-99 0.8008547985542087 1103 100-101 1.0360598580213534 1104 1 0.15249911599464383 1104 2 0.2406883399456774 1104 3 0.2408706183988798 1104 4 0.19765004088944238 1104 5 0.1370791097984423 1104 6 -0.05114158473829633 1104 7 0.09045174995605265 1104 8 -0.005447112004951293 1104 9 0.07656452939301772 1104 10-11 0.09714577562604632 1104 12-13 0.1685709846475163 1104 14-15 0.3352943211887691 1104 16-17 0.24256559314891746 1104 18-19 0.19612180252900657 1104 20-21 0.05502823323931949 1104 22-23 0.09600482572144386 1104 24-25 0.055721647604066504 1104 26-27 0.12144626311103224 1104 28-29 -0.03405536477645654 1104 30-31 0.11100874678650285 1104 32-33 0.039724291013335744 1104 34-35 0.14813887642173995 1104 36-37 0.13706245419277963 1104 38-39 0.02625305285011592 1104 40-41 -0.2360271594310106 1104 42-43 0.06392090046732335 1104 44-45 0.2062725240356329 1104 46-47 0.28460583353404445 1104 48-49 0.25398202187253105 1104 50-51 0.3813332268030081 1104 52-53 0.461396237392357 1104 54-55 0.7668587657314134 1104 56-57 0.725965775863763 1104 58-59 0.5789313729052452 1104 60-61 0.49851691844639134 1104 62-63 0.6551749172889032 1104 64-65 0.7491278264919856 1104 66-67 0.7239679264524455 1104 68-69 0.9741113273239392 1104 70-71 0.9667707388490712 1104 72-73 0.8893389097355886 1104 74-75 0.8907505472768662 1104 76-77 0.9592811696124706 1104 78-79 0.643997306424243 1104 80-81 1.2494592245139025 1104 82-83 1.2723329717920997 1104 84-85 0.9306362552884302 1104 86-87 0.8910554195160643 1104 88-89 1.074848848677636 1104 90-91 1.1660362901868488 1104 92-93 1.072305910693018 1104 94-95 1.4115625327352745 1104 96-97 1.6654900568082844 1104 98-99 1.5387435545350705 1104 100-101 1.8695733839690867 1105 1 0.26977434325000615 1105 2 0.19791085923373686 1105 3 0.03823140061567187 1105 4 0.07243042435899838 1105 5 0.06389906243765608 1105 6 -0.014265104713850008 1105 7 0.008582526825655634 1105 8 0.09007653019197903 1105 9 0.1097361740343743 1105 10-11 0.21359027948366105 1105 12-13 0.27389143381105896 1105 14-15 0.3090644686183026 1105 16-17 -0.02372422428290122 1105 18-19 -0.11397673845334566 1105 20-21 0.37387324126009247 1105 22-23 0.19027760854933717 1105 24-25 -0.18294422971622737 1105 26-27 -0.07093781755506967 1105 28-29 0.10719625847615788 1105 30-31 0.1621316556551875 1105 32-33 0.43946457059472976 1105 34-35 0.46837797198357123 1105 36-37 0.4242158420164728 1105 38-39 0.264594279647163 1105 40-41 0.14034783102044912 1105 42-43 0.053207433199737864 1105 44-45 0.14342400215036832 1105 46-47 0.17107363584097612 1105 48-49 -0.03816525488250022 1105 50-51 0.3623316226474813 1105 52-53 0.3584056331604444 1105 54-55 0.20217692324420966 1105 56-57 0.4036260004455414 1105 58-59 0.37481213067776764 1105 60-61 0.17942364826075874 1105 62-63 0.1482999650316259 1105 64-65 0.16380203014335137 1105 66-67 0.6904525341982293 1105 68-69 0.40957271300875675 1105 70-71 0.8567333085534514 1105 72-73 0.9141269320409897 1105 74-75 0.5288034080208917 1105 76-77 0.3202448087538663 1105 78-79 0.6623400609884484 1105 80-81 0.7894771757781278 1105 82-83 0.9553021872836602 1105 84-85 0.7520020791368758 1105 86-87 0.7692828276590618 1105 88-89 0.8232160475165351 1105 90-91 0.8862066362261132 1105 92-93 0.9457972406143398 1105 94-95 0.9835585605406401 1105 96-97 0.43824423907090804 1105 98-99 1.2419461364615856 1105 100-101 1.145354454933873 1106 1 0.1909489533492561 1106 2 0.12985545515477526 1106 3 0.2207213122745202 1106 4 0.2586830146087564 1106 5 0.2594492860171087 1106 6 0.3719002350178542 1106 7 0.36371940107584777 1106 8 0.23147690100667973 1106 9 0.08587861050509105 1106 10-11 0.26341850502624453 1106 12-13 -0.05070775912165004 1106 14-15 0.2961541185255996 1106 16-17 0.19309331307122335 1106 18-19 0.4209234687666381 1106 20-21 0.40681031182395344 1106 22-23 0.41490679384762785 1106 24-25 0.7792244909730499 1106 26-27 0.42159135683689897 1106 28-29 0.28476469560125395 1106 30-31 0.53553660712209 1106 32-33 0.31464179820951443 1106 34-35 0.41583680064536566 1106 36-37 0.397072751166867 1106 38-39 0.19480749810063713 1106 40-41 0.05589205612678683 1106 42-43 -0.06177239011796587 1106 44-45 -0.2452497899752757 1106 46-47 -0.32911722466725735 1106 48-49 -0.4065805673484135 1106 50-51 -0.2958917386787263 1106 52-53 -0.8969384610701141 1106 54-55 -0.8513458164220609 1106 56-57 -0.7073730179861357 1106 58-59 -0.9541359552639932 1106 60-61 -1.2629092124656012 1106 62-63 -0.7697390795752028 1106 64-65 -0.6868905208436686 1106 66-67 -0.7340485019016754 1106 68-69 -0.6172977199719298 1106 70-71 -0.5104823092892801 1106 72-73 -0.5445381350328482 1106 74-75 -0.7867435436641301 1106 76-77 -0.9123459863164811 1106 78-79 -0.9031932578031316 1106 80-81 -1.0731168911866433 1106 82-83 -1.0078617346271272 1106 84-85 -0.75465077286443 1106 86-87 -0.8558262354885002 1106 88-89 -0.9473832544793233 1106 90-91 -0.47623746891076024 1106 92-93 0.004895833699734453 1106 94-95 0.0031353955280941648 1106 96-97 0.3337753765995366 1106 98-99 0.8627472052804315 1106 100-101 0.9037523172961812 1107 1 0.3063109598154057 1107 2 0.31335697372046667 1107 3 0.06901170122328182 1107 4 -0.13055966198574254 1107 5 -0.13709230308840858 1107 6 -0.03625103371928873 1107 7 0.10620005160979673 1107 8 0.13580726252328645 1107 9 0.20687400582202997 1107 10-11 0.33371340068608646 1107 12-13 0.36719172162685254 1107 14-15 0.2625742223758962 1107 16-17 0.13009732992239975 1107 18-19 0.2640213427746687 1107 20-21 0.4176852016054795 1107 22-23 0.5040368026651052 1107 24-25 0.6605559301814381 1107 26-27 0.6902550190295145 1107 28-29 0.8975304445970451 1107 30-31 0.38742746633635505 1107 32-33 0.35040157091452073 1107 34-35 0.44351388499924127 1107 36-37 0.08623215157835062 1107 38-39 0.0618840142170356 1107 40-41 -0.18188593936650221 1107 42-43 0.09334174723875321 1107 44-45 0.4963980987285552 1107 46-47 0.21320667069866772 1107 48-49 -0.14641597442199128 1107 50-51 -0.582263836258818 1107 52-53 -0.5805134586208212 1107 54-55 -0.37961073265601897 1107 56-57 -0.7255713132736759 1107 58-59 -0.5809361903967414 1107 60-61 -0.4000454914897986 1107 62-63 -0.29349728472853087 1107 64-65 -0.304138487925087 1107 66-67 -0.45377516007743424 1107 68-69 -0.01947269792823647 1107 70-71 0.2512008493273541 1107 72-73 0.2057802739021959 1107 74-75 -0.6655522043871684 1107 76-77 -0.3328172315403499 1107 78-79 -0.33042456964794553 1107 80-81 -0.37925483573450336 1107 82-83 -0.3331532012676668 1107 84-85 -0.20914278814806053 1107 86-87 0.3069787022992898 1107 88-89 0.08364937019002383 1107 90-91 0.367178814032318 1107 92-93 0.47405915299041723 1107 94-95 0.46397109651761426 1107 96-97 0.61265261770858 1107 98-99 0.5777712084155375 1107 100-101 0.4186195652632634 1108 1 -0.2034081346494503 1108 2 -0.14355899167007635 1108 3 -0.08596044527428859 1108 4 -0.2712494806416572 1108 5 -0.0366419060278389 1108 6 -0.090891308698545 1108 7 0.05327839699322112 1108 8 -0.3087227837016755 1108 9 -0.3904947271399237 1108 10-11 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0.18031413300383292 2316 64-65 0.48792845204921775 2316 66-67 0.11727972580642643 2316 68-69 0.29313820582260064 2316 70-71 0.4236906002910743 2316 72-73 0.34883846395297624 2316 74-75 0.27543170309616016 2316 76-77 0.15260466162492392 2316 78-79 -0.008314185997452483 2316 80-81 0.35342888206516676 2316 82-83 0.31527426003074766 2316 84-85 0.27577430173751694 2316 86-87 0.2316406370725872 2316 88-89 0.4291948636734695 2316 90-91 0.4999420765912106 2316 92-93 0.07742144877454749 2316 94-95 0.28207364806873514 2316 96-97 -0.004155801798241043 2316 98-99 -0.1948684538251797 2316 100-101 0.5785504225110145 >>END_MODULE >>Per sequence quality scores pass #Quality Count 3 1.0 4 1.0 5 6.0 6 8.0 7 31.0 8 64.0 9 134.0 10 224.0 11 432.0 12 755.0 13 1157.0 14 1363.0 15 2199.0 16 3295.0 17 5043.0 18 4725.0 19 2477.0 20 1861.0 21 1880.0 22 1825.0 23 2050.0 24 2145.0 25 2543.0 26 2947.0 27 3505.0 28 4137.0 29 4922.0 30 6248.0 31 7843.0 32 10342.0 33 13764.0 34 19996.0 35 30243.0 36 49520.0 37 80561.0 38 73174.0 39 7341.0 40 1.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.36076856897046 20.575853168913106 15.261542873530253 20.801835388586177 2 15.139507344529093 21.487371080074436 40.9108764404481 22.462245134948375 3 19.718261398141433 26.284038157717422 30.391985388358282 23.605715055782866 4 12.534013069046889 17.72349704527143 38.27613594331968 31.46635394236201 5 12.865426639365419 38.83774684401008 33.94675109405739 14.350075422567121 6 30.491479887488065 37.82970097172005 17.296559554769285 14.3822595860226 7 27.56112316960228 34.694620702310736 20.59593477519118 17.148321352895806 8 26.30611618778368 35.47423321854669 19.469668514148577 18.74998207952105 9 26.83942964133237 17.584720856283493 20.983590575835166 34.59225892654897 10-11 24.44668729194324 28.320521385582758 26.891614076034443 20.34117724643956 12-13 26.038909836418117 25.722355235050394 28.002752648707546 20.235982279823943 14-15 22.464252228590762 26.763016719090043 27.488007615486737 23.284723436832465 16-17 20.633209371406945 30.300089172303256 27.841542824210137 21.225158632079662 18-19 20.240822564320183 28.240237639887262 31.735161126610333 19.783778669182226 20-21 21.61998262430361 27.19167457557138 31.519541923885274 19.66880087623974 22-23 21.031549187543188 27.374074212130513 31.414136490450375 20.18024010987593 24-25 21.039932561653615 27.477685419611596 31.746343505475068 19.73603851325972 26-27 20.711832137675547 27.65381549595426 31.913023781260573 19.721328585109614 28-29 20.260209457861123 28.10864282488549 32.0216769531838 19.609470764069588 30-31 20.6403775629869 27.649148562204136 31.683120055739856 20.02735381906911 32-33 20.424471632598642 27.943187780813904 31.503341810914577 20.12899877567288 34-35 20.76080318153015 27.74935988049191 31.65874820436801 19.83108873360993 36-37 21.28236080088771 27.328156943253727 31.616169146383072 19.773313109475488 38-39 20.658728133431588 27.840826005052143 31.612441686761493 19.888004174754776 40-41 20.626109296730963 28.126603910459146 31.432774109193506 19.814512683616385 42-43 20.775662193493993 28.18557620476702 30.875071872351832 20.16368972938715 44-45 20.959535500519692 28.155549980287446 30.49424751801011 20.390667001182752 46-47 21.025309450830505 28.828459440938403 29.967485082993324 20.178746025237768 48-49 21.227165725722053 29.06228585027655 29.470299315007615 20.240249108993787 50-51 21.275766064634148 29.013972239027648 29.3921660267861 20.318095669552104 52-53 21.27920679659253 29.582409831318117 28.833907266539168 20.304476105550187 54-55 20.856856948701555 29.390445660806908 28.940856684912102 20.811840705579435 56-57 21.25669867503147 29.31948056416535 29.102140995461102 20.32167976534208 58-59 21.532243959364955 28.762655442234415 29.0703142248461 20.634786373554533 60-61 20.944738977471808 28.903008633369936 29.805627317117928 20.346625072040325 62-63 20.803238875683487 28.736419861051775 29.91745110576523 20.542890157499507 64-65 20.78159093711202 28.83505417719196 29.81494596617187 20.56840891952415 66-67 20.459165679845626 28.88623506507284 29.626135799955843 21.02846345512569 68-69 20.499307552693377 28.858709209405813 29.56692653750541 21.075056700395397 70-71 20.75621553891898 29.190023024231355 29.262995214515303 20.790766222334366 72-73 20.5758638387673 28.474637504551804 29.56706990133701 21.382428755343888 74-75 20.42934600287301 28.941286776406898 29.114183557315425 21.51518366340466 76-77 20.394651955626028 28.329553306973505 29.515315558129736 21.760479179270735 78-79 20.44898684780209 28.143753781221058 29.069023950361704 22.338235420615145 80-81 20.461518169984117 28.336516019520474 29.221933582213662 21.98003222828175 82-83 20.40131094142913 28.485306388115504 29.309089136562132 21.804293533893226 84-85 19.981792636770273 28.7783862828306 28.892361509347403 22.347459571051726 86-87 20.02405645094233 28.392203301382313 28.579006373955952 23.004733873719402 88-89 20.050865487451365 28.15679988989658 28.937272589122127 22.855062033529933 90-91 19.927734164963976 27.536294225185504 29.28687672509589 23.249094884754633 92-93 20.068758673685902 27.52726834807143 29.08394407551411 23.320028902728556 94-95 19.27899461812176 28.266473221069894 29.033469720125126 23.421062440683215 96-97 19.247597939001558 28.45341965747513 29.37911991811058 22.919862485412732 98-99 19.25319531759629 29.757922882273245 27.94947706468054 23.039404735449924 100-101 18.97714969922494 30.325009141554276 27.13256329181992 23.56527786740086 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 176.0 1 122.0 2 76.0 3 95.0 4 101.0 5 94.0 6 146.0 7 301.0 8 478.0 9 587.5 10 614.5 11 644.5 12 714.0 13 810.5 14 888.0 15 903.0 16 970.5 17 1036.0 18 1045.0 19 1090.5 20 1153.0 21 1212.5 22 1400.5 23 1698.5 24 1961.0 25 2258.5 26 2667.0 27 3229.5 28 3820.0 29 4409.0 30 5158.0 31 6017.0 32 6788.5 33 7404.0 34 8203.0 35 9016.0 36 9943.5 37 10725.5 38 11130.0 39 11615.0 40 12028.5 41 12476.5 42 12833.5 43 13336.5 44 14069.5 45 14012.5 46 13657.5 47 14277.0 48 15405.0 49 14487.0 50 12344.0 51 10932.0 52 9870.0 53 9203.0 54 8433.5 55 7479.5 56 6545.0 57 5602.0 58 4802.0 59 4240.0 60 3463.5 61 2497.5 62 1766.0 63 1295.5 64 891.5 65 581.5 66 434.0 67 385.0 68 275.0 69 152.5 70 107.0 71 76.5 72 52.0 73 31.0 74 22.5 75 22.5 76 17.5 77 11.5 78 9.0 79 5.5 80 3.5 81 2.5 82 2.5 83 3.0 84 2.5 85 1.0 86 0.5 87 0.0 88 0.5 89 0.5 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.018063842781487715 2 0.0 3 0.0 4 0.0 5 0.01749038745509128 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0022938213055857417 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0017203659791893062 24-25 0.0 26-27 2.867276631982177E-4 28-29 0.0015770021475901973 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0017203659791893062 42-43 0.015626657644302867 44-45 1.4336383159910886E-4 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 2.867276631982177E-4 82-83 0.0017203659791893062 84-85 8.601829895946531E-4 86-87 0.0 88-89 0.0 90-91 0.014479746991509994 92-93 0.002007093642387524 94-95 0.0 96-97 0.0 98-99 0.003010640463581286 100-101 0.023081576887456524 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 348763.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 68.50108891285599 #Duplication Level Percentage of deduplicated Percentage of total 1 85.99964583392696 58.910693857439554 2 8.458929163131096 11.588917174223875 3 2.323868287650428 4.77562524582325 4 1.0202221275251482 2.7954530667385313 5 0.5253422835275037 1.7993259236800168 6 0.3593059735417654 1.4767710264302851 7 0.2402464838286839 1.1520002024824787 8 0.17426370109284076 0.9549802626275234 9 0.13680710223686635 0.8434291926813985 >10 0.6939615374597465 8.734438122872453 >50 0.04086580056044238 1.9073109582727146 >100 0.0244352209535487 2.8495670149667287 >500 4.21296912992219E-4 0.15122271782890073 >1k 0.001685187651968876 2.060265233932294 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2613 0.7492193839369429 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1982 0.5682942284588675 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1441 0.41317456266863173 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 1103 0.31626061250763415 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 524 0.15024529551586607 No Hit GCTAAGGGTAGTTTATTTTGGACACAGACATATTAGGGCATTGTCACCAC 443 0.12702035479681045 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 356 0.10207504809856549 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 350 0.1003546821193762 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 2.867276631982177E-4 0.0 24-25 0.0 0.0 0.0 5.734553263964354E-4 0.0 26-27 0.0 0.0 0.0 0.001863729810788415 0.0 28-29 0.0 0.0 0.0 0.005161097937567919 0.0 30-31 0.0 0.0 0.0 0.014479746991509994 0.0 32-33 0.0 0.0 0.0 0.024515215203447613 0.0 34-35 0.0 0.0 0.0 0.03526750257338078 0.0 36-37 0.0 0.0 0.0 0.04716670059610681 0.0 38-39 0.0 0.0 0.0 0.06422699655640077 0.0 40-41 0.0 0.0 0.0 0.08057047335869917 0.0 42-43 0.0 0.0 0.0 0.1245831696596256 0.0 44-45 0.0 0.0 0.0 0.17289678090852528 0.0 46-47 0.0 0.0 0.0 0.22264403047341605 0.0 48-49 0.0 0.0 0.0 0.2936091271149749 0.0 50-51 0.0 0.0 0.0 0.3787672430848456 0.0 52-53 0.0 0.0 0.0 0.5457861068978074 0.0 54-55 0.0 0.0 0.0 0.6811215639273662 0.0 56-57 0.0 0.0 0.0 0.9513623864916863 0.0 58-59 0.0 0.0 0.0 1.2967258568139397 0.0 60-61 0.0 0.0 0.0 1.6521247953481304 0.0 62-63 0.0 0.0 0.0 2.18142406161204 0.0 64-65 0.0 0.0 0.0 2.5022723167308456 0.0 66-67 0.0 0.0 0.0 3.0119307380656775 0.0 68-69 0.0 0.0 0.0 3.604166726401597 0.0 70-71 0.0 0.0 0.0 4.160848484500936 0.0 72-73 0.0 0.0 0.0 5.0703486321656825 0.0 74-75 0.0 0.0 0.0 5.9196359705588035 0.0 76-77 0.0 0.0 0.0 6.547139461468102 0.0 78-79 0.0 0.0 0.0 7.236862855291415 0.0 80-81 0.0 0.0 0.0 8.344348454394531 0.0 82-83 0.0 0.0 0.0 9.075790723213185 0.0 84-85 0.0 0.0 0.0 10.052241780234715 0.0 86-87 0.0 0.0 0.0 11.67167962197825 0.0 88-89 0.0 0.0 0.0 13.754039275955305 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CTAAGGG 160 0.0 47.503944 2 TAAGGGT 170 0.0 44.70959 3 GGTAGTT 170 0.0 44.70959 7 TAGTTTA 210 0.0 38.455574 9 AAGGGTA 200 0.0 38.003155 4 GGGTAGT 240 0.0 35.62285 6 GCTAAGG 255 0.0 35.40017 1 AGGGTAG 215 0.0 35.351772 5 GTAGTTT 245 0.0 31.022984 8 CTAGTAG 65 0.005836195 29.233196 3 TACCTGG 170 3.619789E-9 27.943495 2 ACCGTGG 85 6.3950574E-4 27.943495 8 TAGTCGC 105 6.8883804E-5 27.145111 7 GTCGCTC 110 9.452742E-5 25.911242 9 AGTCGCT 115 1.2785317E-4 24.784666 8 CTAGTCG 120 1.7081886E-4 23.748566 6 GAGTACT 2095 0.0 23.355162 12-13 GTATCAA 3760 0.0 23.123587 1 CTAGTGA 125 2.24808E-4 22.805162 1 GTACTTT 2125 0.0 22.798624 14-15 >>END_MODULE