FastQCFastQC Report
Thu 26 May 2016
SRR937689_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937689_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences299245
Sequences flagged as poor quality0
Sequence length101
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT23650.7903223111497268No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16520.552056007619175No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11480.3836321408879012No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4450.14870758074487458No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT3990.13333556116225836No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT3720.12431285401594011No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN3660.12230780798342496No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT3420.1142876238533643No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3210.10726996273956123No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGACCGC250.00501222657.0155686
GGTATCA14250.032.7029651
GTATCAA21600.030.8212471
AGGGTAG1153.8387934E-628.9257835
GGCTCGG856.3545816E-427.9722251
CCTATAC1205.345848E-627.7205453
GCCCCGC700.00837064327.1502726
AACCGTG700.00837064327.1502727
GTACATG14200.025.7856621
TATCAAC25750.025.282982
TACATGG14250.025.0110152
ATCAACG25850.025.001343
GGGCTAC1151.2690433E-424.8101461
CAACGCA26250.024.80145
TCAACGC26350.024.7072774
AACGCAG26950.024.3294646
CATGGGG5550.023.1182924
GTACTAT1050.002178335622.644181
ACATGGG14700.022.6290133
ACGCAGA29450.021.9414757
Produced by FastQC (version 0.11.5)