##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937689_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 299245 Sequences flagged as poor quality 0 Sequence length 101 %GC 41 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.02909990141857 33.0 31.0 34.0 31.0 34.0 2 32.159524804090296 33.0 31.0 34.0 31.0 34.0 3 32.17445237180237 34.0 31.0 34.0 30.0 34.0 4 35.744750956574045 37.0 35.0 37.0 35.0 37.0 5 35.74879780781634 37.0 35.0 37.0 35.0 37.0 6 35.80756904877274 37.0 36.0 37.0 35.0 37.0 7 35.76563351100269 37.0 35.0 37.0 35.0 37.0 8 35.75860916640211 37.0 35.0 37.0 35.0 37.0 9 37.43063710337683 39.0 37.0 39.0 35.0 39.0 10-11 37.3318785610453 39.0 37.0 39.0 35.0 39.0 12-13 37.225951979147524 39.0 37.0 39.0 34.0 39.0 14-15 38.431490918812344 40.0 38.0 41.0 34.0 41.0 16-17 38.397298200471184 40.0 38.0 41.0 34.0 41.0 18-19 38.38745676619492 40.0 38.0 41.0 34.0 41.0 20-21 38.33841166936791 40.0 38.0 41.0 34.0 41.0 22-23 38.162213236645556 40.0 38.0 41.0 34.0 41.0 24-25 38.024526725592736 40.0 38.0 41.0 33.5 41.0 26-27 37.96249227221842 40.0 38.0 41.0 33.5 41.0 28-29 37.97883506825511 40.0 38.0 41.0 34.0 41.0 30-31 37.773854199736 40.0 37.5 41.0 33.0 41.0 32-33 37.51002188841919 40.0 37.0 41.0 33.0 41.0 34-35 37.5086768367057 40.0 37.0 41.0 33.0 41.0 36-37 37.41727180069843 40.0 37.0 41.0 33.0 41.0 38-39 37.288237063275915 40.0 37.0 41.0 33.0 41.0 40-41 37.0186970542532 40.0 36.5 41.0 31.5 41.0 42-43 36.73316179050611 40.0 36.0 41.0 31.0 41.0 44-45 36.41972129860148 40.0 36.0 41.0 30.5 41.0 46-47 35.957848919781455 39.5 36.0 41.0 29.5 41.0 48-49 35.509171414727064 39.0 35.0 41.0 26.5 41.0 50-51 34.571418068806494 38.5 34.0 40.0 25.0 40.5 52-53 34.649558054437 38.5 35.0 40.0 24.0 40.5 54-55 34.97817507393607 39.0 35.0 40.0 24.0 41.0 56-57 34.76831693094287 39.0 35.0 40.0 23.0 41.0 58-59 34.54642182826781 39.0 35.0 40.0 22.5 41.0 60-61 34.24688633059867 38.0 34.0 40.0 21.5 41.0 62-63 33.91107453758626 38.0 34.0 40.0 19.0 41.0 64-65 33.56591588831894 38.0 34.0 40.0 16.5 41.0 66-67 33.1683904492974 37.0 33.5 40.0 10.5 41.0 68-69 32.684673093953116 36.5 33.0 39.0 7.5 41.0 70-71 32.24346438536985 36.0 32.0 39.0 4.0 40.5 72-73 31.80224230981303 35.5 32.0 39.0 2.0 40.0 74-75 31.340555397751007 35.0 31.0 38.0 2.0 40.0 76-77 30.833360624237663 35.0 31.0 37.0 2.0 39.0 78-79 30.314103827967052 35.0 30.5 37.0 2.0 39.0 80-81 29.80303764473926 34.5 30.0 36.0 2.0 38.5 82-83 29.279891059165564 34.0 29.0 36.0 2.0 37.0 84-85 28.83769319453959 34.0 29.0 35.0 2.0 37.0 86-87 28.396454410265836 34.0 29.0 35.0 2.0 36.5 88-89 27.945950642450168 34.0 27.5 35.0 2.0 36.0 90-91 27.469951043459375 34.0 26.5 35.0 2.0 36.0 92-93 26.960341860348542 33.5 25.5 35.0 2.0 35.0 94-95 26.389764240004013 33.0 25.0 35.0 2.0 35.0 96-97 25.73889455128741 33.0 22.5 35.0 2.0 35.0 98-99 24.844214606760346 32.0 10.5 34.5 2.0 35.0 100-101 22.55744958144664 29.5 2.0 33.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.06642952574147287 1101 2 0.07153284012589012 1101 3 0.1920925135109144 1101 4 -0.09889838452365751 1101 5 0.2846298700654799 1101 6 0.11570307888576536 1101 7 0.116849081752747 1101 8 -0.03829143481449648 1101 9 0.027173703029468754 1101 10-11 -0.1773499015987099 1101 12-13 0.1460841402054811 1101 14-15 0.28477696742033487 1101 16-17 0.2551588417790427 1101 18-19 -0.031015156201398497 1101 20-21 0.27415751620787177 1101 22-23 -0.15462507739635356 1101 24-25 0.05579632658053413 1101 26-27 0.0016617717846756364 1101 28-29 -0.18971451185671384 1101 30-31 -0.16987167113561696 1101 32-33 -0.04134719921952268 1101 34-35 0.08612146506426654 1101 36-37 0.17127525769721075 1101 38-39 0.5025684003263748 1101 40-41 0.6417387647165071 1101 42-43 0.706260508936488 1101 44-45 0.8975439551104003 1101 46-47 1.1778826660515094 1101 48-49 1.080904840964159 1101 50-51 0.7415726512352165 1101 52-53 1.0871872370230733 1101 54-55 1.2398424282854563 1101 56-57 1.3413315022964696 1101 58-59 1.1275422494370133 1101 60-61 1.4170465429339103 1101 62-63 1.6325394306906773 1101 64-65 1.5857715098733323 1101 66-67 1.67203770163745 1101 68-69 2.049029515034462 1101 70-71 1.7181669145396263 1101 72-73 1.6855785829083345 1101 74-75 1.5771057404541153 1101 76-77 1.6004661246264362 1101 78-79 1.6459078417609945 1101 80-81 1.5808613421965774 1101 82-83 1.8170983681244053 1101 84-85 1.6795864172341446 1101 86-87 1.8280555906413873 1101 88-89 1.863028529326872 1101 90-91 1.8705522050697816 1101 92-93 1.341631029432424 1101 94-95 1.7948579827238085 1101 96-97 2.0237486567558456 1101 98-99 1.8388741036850433 1101 100-101 2.436342382689883 1102 1 -0.026197810788300302 1102 2 -0.08266075309209242 1102 3 -0.13123456591372928 1102 4 -0.3655928463400713 1102 5 -0.24556261134651436 1102 6 -0.30080724006357684 1102 7 -0.28506878357975296 1102 8 -0.32617969831251514 1102 9 -0.27975423804481636 1102 10-11 -0.050465043006539645 1102 12-13 -0.18259350554533427 1102 14-15 -0.21536200832839114 1102 16-17 -0.18501209839190125 1102 18-19 0.12998824870444992 1102 20-21 0.11437014910342924 1102 22-23 -0.097123861358547 1102 24-25 -0.048713436465817495 1102 26-27 0.058363877267275655 1102 28-29 -0.16567170732610492 1102 30-31 0.05693675079475469 1102 32-33 -0.11340612493697932 1102 34-35 0.02184518127577917 1102 36-37 -0.14597163272041058 1102 38-39 0.20029614683461006 1102 40-41 -0.02027221436765103 1102 42-43 -0.005121604884649855 1102 44-45 -0.3560867863252142 1102 46-47 -0.2524557398966536 1102 48-49 -0.006649880736908642 1102 50-51 -0.1919621969337726 1102 52-53 0.06821704732117695 1102 54-55 0.0689717452196561 1102 56-57 -0.04387452925102764 1102 58-59 -0.4046653803315863 1102 60-61 0.055952803791392114 1102 62-63 0.12781425523379397 1102 64-65 -0.24565090691493907 1102 66-67 -0.06418632726950335 1102 68-69 0.11744032734771537 1102 70-71 0.10538809444373598 1102 72-73 0.43479684432172405 1102 74-75 0.39626354741680103 1102 76-77 0.35812716277527556 1102 78-79 0.34544227300274244 1102 80-81 0.3640244302377127 1102 82-83 0.01079581791941564 1102 84-85 0.31401071019502425 1102 86-87 0.48370842922105695 1102 88-89 0.01829918459752733 1102 90-91 0.1760556447195647 1102 92-93 0.4906130320729645 1102 94-95 0.4972414168145569 1102 96-97 0.44036320751674296 1102 98-99 0.7319670089730685 1102 100-101 1.51559330340422 1103 1 0.2112042547387638 1103 2 0.14210999120989953 1103 3 0.28429695118028775 1103 4 0.0660533431186181 1103 5 0.2666124242660217 1103 6 0.05796679839796326 1103 7 0.1625807890698212 1103 8 0.09462692561911012 1103 9 -0.13443283491091051 1103 10-11 0.241190494742753 1103 12-13 0.35846663512418075 1103 14-15 0.3931727584094986 1103 16-17 0.5042907862234856 1103 18-19 0.5581510870235391 1103 20-21 0.5362785358555655 1103 22-23 0.617764404310968 1103 24-25 0.4405249004287697 1103 26-27 0.5810835620963317 1103 28-29 0.49921989701862657 1103 30-31 0.6282842401109932 1103 32-33 0.589930324493217 1103 34-35 0.7060454572729356 1103 36-37 0.6972163569519481 1103 38-39 0.8050804684158024 1103 40-41 0.6980882735240925 1103 42-43 1.0269964506709073 1103 44-45 0.5610083436605393 1103 46-47 0.7836742903740017 1103 48-49 0.6825361502460012 1103 50-51 1.0106063571972754 1103 52-53 0.7103611445433984 1103 54-55 0.7517252619575459 1103 56-57 0.9709046730281727 1103 58-59 0.7772924188137083 1103 60-61 0.5286869562130434 1103 62-63 0.7279355604332238 1103 64-65 0.8197759983557162 1103 66-67 0.5668177236564205 1103 68-69 0.9007825638149498 1103 70-71 1.0445240452984343 1103 72-73 1.1311610270140307 1103 74-75 0.9388149630150906 1103 76-77 1.0934369918351052 1103 78-79 1.3600211547691252 1103 80-81 1.3996759170610744 1103 82-83 1.330145666566139 1103 84-85 1.256880412830352 1103 86-87 1.3284155161156441 1103 88-89 1.6477507515490935 1103 90-91 1.8724365207879394 1103 92-93 2.138304894608577 1103 94-95 2.0797178235097142 1103 96-97 2.2237666530295606 1103 98-99 2.444178770012954 1103 100-101 2.6416004704270115 1104 1 0.48982430424371515 1104 2 0.3126313402198022 1104 3 0.1927901442495923 1104 4 0.33871732826712986 1104 5 0.42866378565216223 1104 6 0.41092162513063357 1104 7 0.34698672966388244 1104 8 0.49577172759930477 1104 9 0.0837757046930534 1104 10-11 0.08381889820810073 1104 12-13 0.21426412893605828 1104 14-15 0.552613507171877 1104 16-17 0.44960328622348555 1104 18-19 0.5400895152413625 1104 20-21 0.6298351571426934 1104 22-23 0.5423080305485897 1104 24-25 0.5555078831020381 1104 26-27 0.6082919469973191 1104 28-29 0.4374353982562482 1104 30-31 0.4200153663486148 1104 32-33 0.29642011409718094 1104 34-35 0.5961096590056059 1104 36-37 0.854839345813339 1104 38-39 0.7211347691583754 1104 40-41 0.6359363552072637 1104 42-43 0.8944933566114983 1104 44-45 1.042463325096186 1104 46-47 1.0191515490373675 1104 48-49 0.8275508470281778 1104 50-51 0.5007827185834088 1104 52-53 0.4340036259790452 1104 54-55 0.7602145874525945 1104 56-57 0.7252363561964899 1104 58-59 0.3821036811899461 1104 60-61 0.5450081195793857 1104 62-63 0.6099938834530292 1104 64-65 -0.34038025164428376 1104 66-67 -0.12588416371981737 1104 68-69 0.2771748905476201 1104 70-71 0.04139131014992259 1104 72-73 -0.1362237192730973 1104 74-75 -0.048286676836394093 1104 76-77 0.27629590272619353 1104 78-79 0.2841393478384333 1104 80-81 0.31000620790266 1104 82-83 0.5586999673087121 1104 84-85 -0.041522279001330986 1104 86-87 0.1547614319572297 1104 88-89 0.5283200584797889 1104 90-91 0.4283616445503178 1104 92-93 0.8259209218362997 1104 94-95 1.344530632915653 1104 96-97 1.65939087951471 1104 98-99 1.8779078851119664 1104 100-101 2.693754709288399 1105 1 -0.132856175724811 1105 2 -0.24729915614109288 1105 3 -0.2995710123958695 1105 4 -0.3714983952919795 1105 5 -0.4914070293763615 1105 6 -0.25241192676759994 1105 7 -0.34818080033415555 1105 8 -0.42410237901665226 1105 9 -0.48258059981156975 1105 10-11 -0.2991395963168486 1105 12-13 -0.38877829451157453 1105 14-15 -0.6381936530143406 1105 16-17 -0.048741084637441645 1105 18-19 -0.47043438483076017 1105 20-21 -0.41775188632986016 1105 22-23 -0.21721593509300874 1105 24-25 -0.5975804389752 1105 26-27 -0.20299911588380581 1105 28-29 -0.7840090971866758 1105 30-31 -0.5348728377035812 1105 32-33 -0.4338434751756566 1105 34-35 -0.7708662769985892 1105 36-37 -0.4336171488427567 1105 38-39 -0.6925266300941288 1105 40-41 -0.5572149135619995 1105 42-43 -0.19791042019001992 1105 44-45 -0.26666394524674786 1105 46-47 -0.6369201839637455 1105 48-49 -0.8291437959460524 1105 50-51 -0.8033940567100117 1105 52-53 -0.9016137105890465 1105 54-55 -0.8538598994663005 1105 56-57 -1.0204032739916897 1105 58-59 -1.2247253791995334 1105 60-61 -0.5863275305419222 1105 62-63 -0.9883439304608146 1105 64-65 -0.9347511125714405 1105 66-67 -0.9064723218733803 1105 68-69 -0.7454301845294218 1105 70-71 -0.45865786695322086 1105 72-73 -0.7475770731515325 1105 74-75 -0.46214478450146856 1105 76-77 -0.9518858558470136 1105 78-79 -0.2529237955620012 1105 80-81 -0.18805949270713995 1105 82-83 0.07550214421514667 1105 84-85 -0.3285526376663377 1105 86-87 -0.14131751368568146 1105 88-89 0.1945222747278983 1105 90-91 0.09185912012568664 1105 92-93 0.1329499691118876 1105 94-95 0.43208226887395185 1105 96-97 1.0721343061752542 1105 98-99 1.5517817294500418 1105 100-101 1.903642585493234 1106 1 0.5222838001933106 1106 2 0.3093685139371729 1106 3 0.39154126936210787 1106 4 0.25000220675498497 1106 5 0.3411862879023886 1106 6 0.5046997529434165 1106 7 0.3387171527061881 1106 8 0.286388289255477 1106 9 0.39042511963454274 1106 10-11 0.31967060837911276 1106 12-13 0.14841691921508726 1106 14-15 -0.1673741165905014 1106 16-17 -0.09016944104924107 1106 18-19 0.0933073370235391 1106 20-21 0.12896319494647202 1106 22-23 0.020818381583694645 1106 24-25 0.36772660497422294 1106 26-27 0.3085338461872382 1106 28-29 0.21992302201862657 1106 30-31 0.29341208102008665 1106 32-33 0.36727407449321703 1106 34-35 0.4427286959092953 1106 36-37 0.2329052774064948 1106 38-39 -0.0661908384023775 1106 40-41 0.277278614433186 1106 42-43 0.38850213248909427 1106 44-45 0.6290126050241795 1106 46-47 0.1812240062830952 1106 48-49 0.11524211615509472 1106 50-51 0.39625976628818194 1106 52-53 0.6313483604524919 1106 54-55 0.653891455139366 1106 56-57 0.482268309391813 1106 58-59 0.3745935551773485 1106 60-61 0.5720108198494032 1106 62-63 0.8023318672514108 1106 64-65 0.585756111992076 1106 66-67 0.6543532350200536 1106 68-69 0.3510666547240433 1106 70-71 0.050560977116614225 1106 72-73 0.2510118792867573 1106 74-75 0.8175436561969107 1106 76-77 0.9883233554714685 1106 78-79 1.0425495638600317 1106 80-81 0.6320977920610744 1106 82-83 0.0355789052025024 1106 84-85 0.05925967419398859 1106 86-87 0.43423937975200744 1106 88-89 0.6108189333672769 1106 90-91 0.6884876571515761 1106 92-93 0.5122395536994837 1106 94-95 0.5800729371460775 1106 96-97 1.1131487553022872 1106 98-99 1.447552349558407 1106 100-101 1.6059115499724648 1107 1 -0.22021272999405994 1107 2 0.018511470217532633 1107 3 -0.4423848236288741 1107 4 -0.2333860652783315 1107 5 -0.20613328184084878 1107 6 -0.36539913671654034 1107 7 -0.10936577581567519 1107 8 0.21378245997025402 1107 9 -0.04074247231549322 1107 10-11 -0.1482119384633549 1107 12-13 -0.029519409723143042 1107 14-15 0.142024738371326 1107 16-17 0.020213973246384853 1107 18-19 -0.1786392278619573 1107 20-21 -0.1534578668161899 1107 22-23 -0.00511846782643488 1107 24-25 0.2481048145509135 1107 26-27 -0.3932574445830568 1107 28-29 -0.21113613828671163 1107 30-31 -0.33733479614854645 1107 32-33 -0.7765713453541068 1107 34-35 -0.8729591944446184 1107 36-37 -1.5026733138495771 1107 38-39 -1.6336570077292336 1107 40-41 -1.293830858155296 1107 42-43 -1.8465126142145891 1107 44-45 -2.238505611492137 1107 46-47 -2.944513328900804 1107 48-49 -3.257155524372319 1107 50-51 -3.000426562650052 1107 52-53 -2.392766837326832 1107 54-55 -2.833183492813447 1107 56-57 -3.272893036895489 1107 58-59 -3.044451378896216 1107 60-61 -3.4862820323365753 1107 62-63 -3.266413795482805 1107 64-65 -2.910990938667183 1107 66-67 -3.3138329204275507 1107 68-69 -3.46288632931482 1107 70-71 -3.2968106398160693 1107 72-73 -2.959800924321847 1107 74-75 -2.874206864274985 1107 76-77 -3.2746450692335998 1107 78-79 -3.085768444467515 1107 80-81 -2.71308350564885 1107 82-83 -2.4602139469834796 1107 84-85 -2.636979916368123 1107 86-87 -2.9255801899912264 1107 88-89 -3.1323065003593022 1107 90-91 -3.2723034601280894 1107 92-93 -3.518988759971574 1107 94-95 -2.6246893806887606 1107 96-97 -2.9681316055582236 1107 98-99 -2.4812297462084203 1107 100-101 -1.8221548969393986 1108 1 0.18548310089260767 1108 2 -0.2114356818670231 1108 3 -0.3910636257427882 1108 4 0.08540430465708226 1108 5 0.02081915503525522 1108 6 0.07611583685950052 1108 7 0.3029654044544401 1108 8 0.4669826948498823 1108 9 0.5695335112429376 1108 10-11 0.4680534755119794 1108 12-13 0.5523067793549501 1108 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1170.0 3 223.0 4 163.0 5 224.0 6 211.0 7 243.0 8 273.0 9 336.0 10 479.0 11 661.0 12 972.0 13 1398.0 14 1625.0 15 2271.0 16 3084.0 17 4544.0 18 3843.0 19 2192.0 20 2082.0 21 2041.0 22 2178.0 23 2328.0 24 2567.0 25 2863.0 26 3196.0 27 3515.0 28 4194.0 29 4739.0 30 5741.0 31 7248.0 32 9423.0 33 12674.0 34 17832.0 35 26277.0 36 40235.0 37 61167.0 38 56554.0 39 8473.0 40 6.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.23926816112248 19.69036555090609 15.408350950311744 21.662015337659685 2 14.346342067976808 20.218193024120477 42.68463839379922 22.750826514103494 3 18.867204115603993 25.741615897056313 30.638608572165417 24.75257141517428 4 11.745614945858772 16.71997615307582 39.031252197969664 32.50315670309575 5 12.432055889159418 37.71279107541755 35.19931947044619 14.655833564976842 6 29.617577443664402 37.196393718132846 18.473657604092388 14.712371234110364 7 26.6823108802046 34.102488568119284 21.661656501275417 17.553544050400703 8 25.09474642775836 34.86032434748838 20.831826764694068 19.213102460059194 9 25.65478681554146 17.203743073806855 21.91003900430219 35.2314311063495 10-11 23.595221593895747 27.392802257957594 28.1618604713453 20.850115676801362 12-13 24.969407006506973 25.123083438937055 29.154662439169744 20.752847115386224 14-15 21.756387120966096 25.83440189897786 28.54091406723426 23.868296912821783 16-17 19.77954100336464 29.747401195199114 28.927836086978353 21.54522171445789 18-19 19.685401834960672 27.456281166460496 32.70270858407391 20.15560841450493 20-21 20.49932295476267 26.488955542648828 32.80999717130667 20.201724331281834 22-23 20.292489271822888 26.61290862471799 32.667215627991666 20.427386475467454 24-25 20.017439651608647 26.723839267333794 32.88646318174895 20.372257899308607 26-27 19.90753991567453 26.846398090541925 32.835883350322284 20.41017864346126 28-29 19.524215269907646 27.20436713533131 33.066888081276126 20.20452951348492 30-31 19.599404764893848 26.880321922428614 33.01161177146052 20.50866154121702 32-33 19.453181627197292 27.197626983024293 32.78951694306668 20.55967444671174 34-35 19.957182479967997 27.096727757235712 32.55755173504692 20.388538027749366 36-37 20.007966900540946 26.83556718975952 32.671321836002356 20.485144073697178 38-39 19.66933240495566 27.174629848457965 32.775358267861506 20.380679478724865 40-41 19.7900592801363 27.334803900237993 32.57194118178684 20.30319563783887 42-43 19.76459442585578 27.33744311115649 32.033837877466574 20.86412458552115 44-45 19.72939181781816 27.539169396236456 31.87512657560476 20.85631221034062 46-47 19.96414948759877 27.777452337396504 31.382587898192575 20.87581027681215 48-49 20.130159250914765 28.00951421273405 30.94261677675077 20.917709759600417 50-51 20.055108777897562 28.084049256399783 30.91498650738913 20.945855458313524 52-53 20.309978370487432 28.38187138810578 30.370380291027438 20.937769950379355 54-55 19.993907307381363 28.05283234885017 30.702149347964724 21.25111099580374 56-57 20.132673757773333 28.099625809129186 30.462082214888646 21.30561821820884 58-59 20.440985732814525 27.667963683527887 30.60123007405548 21.28982050960211 60-61 20.004954124140976 27.547942866898083 31.305712975453652 21.141390033507285 62-63 19.989756567847675 27.323853137302162 31.513952491898984 21.17243780295118 64-65 19.856237918713337 27.401655188572672 31.438541291850413 21.30356560086358 66-67 19.7452719665272 27.272970711297074 31.197991631799166 21.78376569037657 68-69 19.676230454823905 27.309184517174312 31.38559439763533 21.628990630366452 70-71 19.73370825069251 27.361435397889416 31.108098382332017 21.796757969086055 72-73 19.56206651849075 26.954516241330367 31.17301100607878 22.3104062341001 74-75 19.498495352161235 27.050354655035637 31.2883065933367 22.16284339946643 76-77 19.543210083189663 26.86724804579616 31.289690842441793 22.299851028572384 78-79 19.339427611062103 26.428754366905427 31.126347735402778 23.105470286629696 80-81 19.557812596767203 26.478184383677668 31.376592421987855 22.587410597567285 82-83 19.548300813647725 26.60448954108662 31.43833701854434 22.408872626721323 84-85 19.31460993857432 26.425427218103003 30.90464793211375 23.35531491120893 86-87 19.339559483162617 26.296277699671954 31.114179554127336 23.249983263038093 88-89 19.349066867520296 25.828437526152815 31.143526654950204 23.67896895137668 90-91 19.32767483082238 25.10942356586085 31.875021968402322 23.687879634914445 92-93 19.279094717567883 25.294114693432817 31.556343320428944 23.87044726857036 94-95 18.43744194259451 25.467597482045097 31.840313053899035 24.254647521461365 96-97 18.252458056148278 25.410986987992136 32.35713986862475 23.97941508723484 98-99 18.721286947265888 26.988135703849498 30.986917308081747 23.30366004080287 100-101 18.591270206538056 27.175461116587478 30.172865374541647 24.06040330233282 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 391.0 1 230.0 2 83.0 3 88.5 4 71.5 5 74.0 6 130.5 7 281.5 8 470.5 9 589.5 10 593.0 11 564.5 12 584.5 13 653.5 14 723.0 15 738.5 16 770.5 17 811.0 18 860.0 19 922.5 20 1002.0 21 1104.0 22 1308.0 23 1554.0 24 1746.5 25 2103.0 26 2578.5 27 3125.5 28 3558.5 29 4033.0 30 4630.0 31 5154.5 32 5792.5 33 6450.0 34 7302.0 35 8191.0 36 8873.5 37 9467.0 38 9824.5 39 9917.5 40 10113.0 41 10678.0 42 11264.5 43 11590.5 44 11913.5 45 12132.0 46 11972.0 47 12082.5 48 12886.5 49 12308.0 50 10519.0 51 9273.5 52 8331.5 53 7804.5 54 7120.0 55 6183.0 56 5317.0 57 4473.5 58 3777.0 59 3105.0 60 2495.0 61 1927.5 62 1376.0 63 980.5 64 682.0 65 493.5 66 376.5 67 278.5 68 192.0 69 120.5 70 88.5 71 63.0 72 45.0 73 33.5 74 27.5 75 21.5 76 12.0 77 7.5 78 6.0 79 5.5 80 3.5 81 1.5 82 1.5 83 1.0 84 1.5 85 1.5 86 1.5 87 1.5 88 0.0 89 0.5 90 0.5 91 0.5 92 0.5 93 0.0 94 0.0 95 0.0 96 0.5 97 0.5 98 0.0 99 0.5 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.2553091948069308 2 0.2345903858042741 3 0.2252335043192033 4 0.2252335043192033 5 0.2185500175441528 6 0.1817908402813748 7 0.1734364818125616 8 0.18479840933014755 9 0.18713762970141523 10-11 0.18981102441143546 12-13 0.18897558856455413 14-15 0.18646928102391017 16-17 0.18379588631388996 18-19 0.1826262761282561 20-21 0.1739377433206904 22-23 0.16675299503751106 24-25 0.16725425654563986 26-27 0.17427191765944294 28-29 0.18697054253203899 30-31 0.18028705575698842 32-33 0.18496549649952382 34-35 0.17895035840197832 36-37 0.17009473842503633 38-39 0.17243395879630402 40-41 0.16508212334374844 42-43 0.17594278935320556 44-45 0.17059599993316513 46-47 0.16859095390065 48-49 0.17844909689384952 50-51 0.18814015271767282 52-53 0.19348694213771325 54-55 0.17594278935320556 56-57 0.1560594161974302 58-59 0.16123911844809435 60-61 0.16842386673127369 62-63 0.17276813313505654 64-65 0.16290999014185703 66-67 0.16541629768250096 68-69 0.17176561011879898 70-71 0.1712643486106702 72-73 0.16742134371501613 74-75 0.16842386673127369 76-77 0.17711239953883942 78-79 0.1836287991445137 80-81 0.17560861501445305 82-83 0.17661113803071063 84-85 0.17042891276378885 86-87 0.16875804107002623 88-89 0.17377065615131415 90-91 0.1739377433206904 92-93 0.1702618255944126 94-95 0.17059599993316513 96-97 0.16123911844809435 98-99 0.1642466874968671 100-101 0.16257581580310448 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 299245.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 73.26873589361062 #Duplication Level Percentage of deduplicated Percentage of total 1 88.34599842631522 64.72999625955029 2 7.130944892787398 10.449506360430629 3 1.805582870771009 3.9687832347764505 4 0.813283725281402 2.383530818968593 5 0.47646864342133094 1.7455127598212212 6 0.30255181831441563 1.3300553562126352 7 0.21344518642253266 1.0947201294228521 8 0.16328878246530015 0.9571170141471451 9 0.11546101414850027 0.7613714296489416 >10 0.5882406002423395 7.490086344335483 >50 0.03058347621070402 1.5401406287585728 >100 0.012781154237307808 1.8217472323229704 >500 0.0 0.0 >1k 0.0013694093825686938 1.727432431604212 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2365 0.7903223111497268 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1652 0.552056007619175 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1148 0.3836321408879012 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 445 0.14870758074487458 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 399 0.13333556116225836 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 372 0.12431285401594011 No Hit NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 366 0.12230780798342496 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 342 0.1142876238533643 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 321 0.10726996273956123 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 3.341743387525272E-4 0.0 14-15 0.0 0.0 0.0 3.341743387525272E-4 0.0 16-17 0.0 0.0 0.0 3.341743387525272E-4 0.0 18-19 0.0 0.0 0.0 3.341743387525272E-4 0.0 20-21 0.0 0.0 0.0 3.341743387525272E-4 0.0 22-23 0.0 0.0 0.0 3.341743387525272E-4 0.0 24-25 0.0 0.0 0.0 0.0010025230162575816 0.0 26-27 0.0 0.0 0.0 0.0015037845243863724 0.0 28-29 0.0 0.0 0.0 0.005179702250664171 0.0 30-31 0.0 0.0 0.0 0.014369496566358669 0.0 32-33 0.0 0.0 0.0 0.025731424083944594 0.0 34-35 0.0 0.0 0.0 0.035756654246520414 0.0 36-37 0.0 0.0 0.0 0.050293237982255344 0.0 38-39 0.0 0.0 0.0 0.06633360624237665 0.0 40-41 0.0 0.0 0.0 0.08638406656752828 0.0 42-43 0.0 0.0 0.0 0.13166468946849572 0.0 44-45 0.0 0.0 0.0 0.18613510668515765 0.0 46-47 0.0 0.0 0.0 0.24511687747497868 0.0 48-49 0.0 0.0 0.0 0.31696436030677205 0.0 50-51 0.0 0.0 0.0 0.408862303463717 0.0 52-53 0.0 0.0 0.0 0.5929923641163595 0.0 54-55 0.0 0.0 0.0 0.7396948988287189 0.0 56-57 0.0 0.0 0.0 1.0446289829404 0.0 58-59 0.0 0.0 0.0 1.4369496566358668 0.0 60-61 0.0 0.0 0.0 1.825594412605056 0.0 62-63 0.0 0.0 0.0 2.4092298952363445 0.0 64-65 0.0 0.0 0.0 2.730705609116276 0.0 66-67 0.0 0.0 0.0 3.314508178916941 0.0 68-69 0.0 0.0 0.0 3.936072448996642 0.0 70-71 0.0 0.0 0.0 4.522214239168575 0.0 72-73 0.0 0.0 0.0 5.534261224080603 0.0 74-75 0.0 0.0 0.0 6.438704071914318 0.0 76-77 0.0 0.0 0.0 7.108890708282511 0.0 78-79 0.0 0.0 0.0 7.860782970475697 0.0 80-81 0.0 0.0 0.0 9.131480893582182 0.0 82-83 0.0 0.0 0.0 9.914618456448729 0.0 84-85 0.0 0.0 0.0 10.954067737138466 0.0 86-87 0.0 0.0 0.0 12.70146535447543 0.0 88-89 0.0 0.0 0.0 14.964828150846296 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGACCGC 25 0.005012226 57.015568 6 GGTATCA 1425 0.0 32.702965 1 GTATCAA 2160 0.0 30.821247 1 AGGGTAG 115 3.8387934E-6 28.925783 5 GGCTCGG 85 6.3545816E-4 27.972225 1 CCTATAC 120 5.345848E-6 27.720545 3 GCCCCGC 70 0.008370643 27.150272 6 AACCGTG 70 0.008370643 27.150272 7 GTACATG 1420 0.0 25.785662 1 TATCAAC 2575 0.0 25.28298 2 TACATGG 1425 0.0 25.011015 2 ATCAACG 2585 0.0 25.00134 3 GGGCTAC 115 1.2690433E-4 24.810146 1 CAACGCA 2625 0.0 24.8014 5 TCAACGC 2635 0.0 24.707277 4 AACGCAG 2695 0.0 24.329464 6 CATGGGG 555 0.0 23.118292 4 GTACTAT 105 0.0021783356 22.64418 1 ACATGGG 1470 0.0 22.629013 3 ACGCAGA 2945 0.0 21.941475 7 >>END_MODULE