FastQCFastQC Report
Thu 26 May 2016
SRR937689_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937689_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences299245
Sequences flagged as poor quality0
Sequence length101
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT22190.7415328576918578No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16750.5597420174104831No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12250.4093635649718458No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT9410.3144580527661281No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4290.14336079132483417No Hit
GCTAAGGGTAGTTTATTTTGGACACAGACATATTAGGGCATTGTCACCAC4160.13901652492105132No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3110.10392821935203596No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGTCGG250.001611026138.02000440-41
TATAGAC651.3269494E-436.5332573
AGGGTAG1456.6029315E-1032.7539525
TTAGTCG300.003939289631.68333238-39
GACCGCG600.003945845631.6674487
CTGGTCG600.003945845631.6674489
CTAAGGG1509.513315E-1031.6621552
TAAGGGT1701.07320375E-1030.7309153
GCTTATA1401.688386E-830.5517861
ATAGGAG1252.1475898E-730.3956685
AAGGGTA1751.5097612E-1029.8528884
GTAAGGT804.4895307E-429.6832714
GCTAAGG1652.6157068E-928.803031
CTGAACG856.395098E-427.9418649
GGGTAGT1903.929017E-1027.4960826
GTATCAA30050.026.8860241
TAAGGTG1257.3751835E-626.596215
CGTGTGA458.9150807E-426.40277738-39
GAACGTT458.941229E-426.38953818-19
TATAGGC908.939459E-426.3851283
Produced by FastQC (version 0.11.5)