##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937689_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 299245 Sequences flagged as poor quality 0 Sequence length 101 %GC 42 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.420762251666694 33.0 31.0 34.0 31.0 34.0 2 32.6284749954051 34.0 31.0 34.0 31.0 34.0 3 32.6941603034303 34.0 31.0 34.0 31.0 34.0 4 36.240164413774664 37.0 35.0 37.0 35.0 37.0 5 36.1883473408077 37.0 35.0 37.0 35.0 37.0 6 36.241975638690704 37.0 37.0 37.0 35.0 37.0 7 36.20555063576668 37.0 36.0 37.0 35.0 37.0 8 36.172988688198636 37.0 36.0 37.0 35.0 37.0 9 37.88084345603101 39.0 38.0 39.0 35.0 39.0 10-11 37.820289394977365 39.0 38.0 39.0 35.0 39.0 12-13 37.72241641464352 39.0 37.0 39.0 35.0 39.0 14-15 38.96978729803338 40.0 38.0 41.0 36.0 41.0 16-17 38.95142775986232 40.0 38.0 41.0 36.0 41.0 18-19 38.952478738157694 40.0 38.0 41.0 35.5 41.0 20-21 38.869023709669335 40.0 38.0 41.0 35.0 41.0 22-23 38.810162241641464 40.0 38.0 41.0 35.0 41.0 24-25 38.81525171682067 40.0 38.0 41.0 35.0 41.0 26-27 38.63692125181707 40.0 38.0 41.0 34.5 41.0 28-29 38.585082457518084 40.0 38.0 41.0 34.5 41.0 30-31 38.57020835770021 40.0 38.0 41.0 35.0 41.0 32-33 38.40950057645074 40.0 38.0 41.0 34.5 41.0 34-35 38.235198583100804 40.0 38.0 41.0 34.0 41.0 36-37 38.06046216311049 40.0 37.5 41.0 34.0 41.0 38-39 37.902282410733676 40.0 37.0 41.0 33.5 41.0 40-41 37.695092649835416 40.0 37.0 41.0 33.0 41.0 42-43 37.418581764106335 40.0 37.0 41.0 33.0 41.0 44-45 37.01338201139534 40.0 37.0 41.0 31.5 41.0 46-47 36.54038329796654 40.0 36.5 41.0 30.5 41.0 48-49 36.08480676368862 40.0 36.0 41.0 29.5 41.0 50-51 35.77432538555364 39.5 36.0 41.0 28.0 41.0 52-53 35.49966081304616 39.0 35.0 41.0 27.0 41.0 54-55 35.25802603218099 39.0 35.0 40.5 26.0 41.0 56-57 34.98662467209143 39.0 35.0 40.0 25.5 41.0 58-59 34.696955671773964 38.5 35.0 40.0 24.5 41.0 60-61 34.33209911610888 38.0 34.0 40.0 23.0 41.0 62-63 33.92096108539825 38.0 34.0 40.0 21.5 41.0 64-65 33.535131748233056 37.5 34.0 40.0 19.5 41.0 66-67 33.11689251282394 37.0 33.0 39.5 17.0 41.0 68-69 32.630119133151766 36.0 33.0 39.0 12.0 40.5 70-71 32.175538438403315 36.0 32.0 39.0 7.5 40.0 72-73 31.69130478370566 35.0 31.5 38.0 4.5 40.0 74-75 31.185730755735268 35.0 31.0 37.5 2.0 39.5 76-77 29.94734582031446 34.0 29.5 36.0 2.0 39.0 78-79 30.484728232719007 34.5 31.0 36.0 2.0 39.0 80-81 30.287971394676603 35.0 31.0 36.0 2.0 38.5 82-83 29.886317231699778 34.0 30.5 36.0 2.0 37.0 84-85 29.49873013751274 34.0 30.0 35.0 2.0 37.0 86-87 29.18420023726378 34.0 30.0 35.0 2.0 36.0 88-89 28.82303296629852 34.0 29.5 35.0 2.0 36.0 90-91 28.388718274323715 34.0 29.0 35.0 2.0 35.5 92-93 28.00874701331685 34.0 29.0 35.0 2.0 35.0 94-95 27.60855319220037 34.0 27.5 35.0 2.0 35.0 96-97 27.191032431619576 34.0 27.0 35.0 2.0 35.0 98-99 26.523880098247254 33.0 25.5 35.0 2.0 35.0 100-101 24.317729619542515 31.0 19.5 34.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.15469233389603687 1101 2 0.22626818765662193 1101 3 0.2510965338534277 1101 4 0.08152989623728502 1101 5 0.11437349046319412 1101 6 0.10140480935784524 1101 7 0.04426643218852888 1101 8 0.07682457993490743 1101 9 -0.07533915965673543 1101 10-11 0.12460922062019364 1101 12-13 0.1355113539302124 1101 14-15 0.3185446950320099 1101 16-17 0.29431371454663946 1101 18-19 0.205984577650959 1101 20-21 0.3185161710042692 1101 22-23 0.2699709468699183 1101 24-25 0.0844785263753991 1101 26-27 0.04308882201627995 1101 28-29 0.16151636795409274 1101 30-31 0.19113113331093246 1101 32-33 0.28049565932676757 1101 34-35 0.27801312162264935 1101 36-37 0.262262946899547 1101 38-39 0.2180728043642688 1101 40-41 0.45305961605628653 1101 42-43 0.5461553294930326 1101 44-45 0.9497264227261653 1101 46-47 0.9010503246120507 1101 48-49 0.9826983456136205 1101 50-51 0.8612283312503948 1101 52-53 0.9659331327825527 1101 54-55 1.0439822495001465 1101 56-57 0.8729913469158319 1101 58-59 1.1218388565338202 1101 60-61 0.8682945601079197 1101 62-63 0.70273015612851 1101 64-65 1.0555510916878177 1101 66-67 1.2827184506322666 1101 68-69 1.4547327784404231 1101 70-71 0.8399397131130826 1101 72-73 1.0221003900944723 1101 74-75 1.103246591450894 1101 76-77 0.8638531828228118 1101 78-79 1.0203130400583085 1101 80-81 1.0685473768608418 1101 82-83 1.10296642327258 1101 84-85 1.3705805658914656 1101 86-87 1.6875446998187869 1101 88-89 1.298654561606849 1101 90-91 1.3743423033012547 1101 92-93 1.3789369351041074 1101 94-95 1.318623133052558 1101 96-97 1.1479679513695267 1101 98-99 0.7776816208556276 1101 100-101 0.9620430730571492 1102 1 0.15170435529829973 1102 2 -0.04883430695807078 1102 3 -0.11058785222120093 1102 4 9.010748041760053E-5 1102 5 -0.06692037375749749 1102 6 0.0586697666227991 1102 7 -0.10520198557257032 1102 8 0.014702420251772708 1102 9 -0.07123937506914757 1102 10-11 0.04435072572754706 1102 12-13 0.04163323566184829 1102 14-15 -0.08625691708667205 1102 16-17 -0.12243981196298392 1102 18-19 0.1228075920349525 1102 20-21 -0.1200912919934396 1102 22-23 -0.10797360180634286 1102 24-25 -0.09830932714416463 1102 26-27 -0.07556172346352952 1102 28-29 -0.021236741628285927 1102 30-31 -0.15154831912462186 1102 32-33 0.0601843536496105 1102 34-35 0.17961829152050512 1102 36-37 -0.054393296585963924 1102 38-39 0.19086830155001167 1102 40-41 0.11659932837648768 1102 42-43 0.22491288629936435 1102 44-45 0.19303821481845063 1102 46-47 0.16173408529580513 1102 48-49 0.0807109924067575 1102 50-51 0.3113707813928457 1102 52-53 0.4711794672971763 1102 54-55 0.4686573936874865 1102 56-57 0.4548828068560766 1102 58-59 0.27512910738504104 1102 60-61 0.42711604575868023 1102 62-63 0.7138134720898748 1102 64-65 0.6994604794996491 1102 66-67 0.5169968190569776 1102 68-69 0.39695361090516457 1102 70-71 0.5488897513314086 1102 72-73 0.8817696278124885 1102 74-75 1.0534932734049 1102 76-77 0.835015714960953 1102 78-79 0.613357303834281 1102 80-81 0.7816319436039478 1102 82-83 0.7677221733941835 1102 84-85 0.8212094311544753 1102 86-87 1.0050347977966894 1102 88-89 0.7298615659845815 1102 90-91 0.823650898951314 1102 92-93 1.110574764302914 1102 94-95 1.0267740607156846 1102 96-97 1.024835437993108 1102 98-99 0.6330078664257783 1102 100-101 1.0320521064808155 1103 1 0.19169055544075064 1103 2 0.3264439159764052 1103 3 0.37512261786427104 1103 4 0.02290438810720019 1103 5 0.1483017594333873 1103 6 0.061040226024509536 1103 7 0.12817141186332748 1103 8 0.2578247493116024 1103 9 0.0013990686630478422 1103 10-11 0.3185561210266954 1103 12-13 0.2428273684966058 1103 14-15 0.5381309924574893 1103 16-17 0.4095057453732025 1103 18-19 0.27672051938537834 1103 20-21 0.328826977237334 1103 22-23 0.09778895161246481 1103 24-25 0.14341632278460992 1103 26-27 0.06337168109487124 1103 28-29 0.3469467964771269 1103 30-31 0.4020262891374884 1103 32-33 0.19114945336470868 1103 34-35 0.3800890447256293 1103 36-37 0.29925587542258114 1103 38-39 0.32733665090898256 1103 40-41 0.5153373091457141 1103 42-43 0.6593413051027852 1103 44-45 0.8640006435933785 1103 46-47 0.5490537968342721 1103 48-49 0.6558701359252765 1103 50-51 0.6511588868059519 1103 52-53 0.8430132903028777 1103 54-55 0.8919402017359204 1103 56-57 1.058008919747813 1103 58-59 0.9972622982682324 1103 60-61 0.657039478807107 1103 62-63 0.6194179948818999 1103 64-65 0.8764881682460839 1103 66-67 0.9933701274615387 1103 68-69 0.4998293231558719 1103 70-71 0.7063861900778505 1103 72-73 0.7214408795931178 1103 74-75 0.6869345572368069 1103 76-77 0.7977115839068212 1103 78-79 0.9082068408713191 1103 80-81 0.9687368670369949 1103 82-83 1.0634063792346389 1103 84-85 1.0032395682627389 1103 86-87 1.3013923031385701 1103 88-89 1.3234947123521046 1103 90-91 1.4984583686806587 1103 92-93 1.654099960171859 1103 94-95 1.7247408075105533 1103 96-97 1.7439304344318565 1103 98-99 1.4770750405710764 1103 100-101 2.0280735185178536 1104 1 0.2652131420744155 1104 2 0.2557833342932341 1104 3 0.1961312812306062 1104 4 0.19160797721610834 1104 5 0.19745862824527194 1104 6 0.23341801067797263 1104 7 0.1041150999821383 1104 8 0.2596038334700168 1104 9 0.16209677905908393 1104 10-11 0.08568947498709178 1104 12-13 0.28461650834809404 1104 14-15 0.2935842721604587 1104 16-17 0.1663320139375557 1104 18-19 0.4635243560190432 1104 20-21 0.28280284357396823 1104 22-23 0.42811846893919636 1104 24-25 0.31275806164600084 1104 26-27 0.1928580672334803 1104 28-29 0.37701718632861514 1104 30-31 0.43196131388996406 1104 32-33 0.06336479989936095 1104 34-35 0.2781204494285987 1104 36-37 0.6114271316107036 1104 38-39 0.5538411372951231 1104 40-41 0.7582596482546293 1104 42-43 0.73282521599387 1104 44-45 0.4039913614151587 1104 46-47 0.26683690326991893 1104 48-49 0.13635822379656304 1104 50-51 -0.13024581616434006 1104 52-53 -0.07338909212286637 1104 54-55 0.4363592920824573 1104 56-57 0.14079434672800772 1104 58-59 0.09967566460486665 1104 60-61 0.3997684392031431 1104 62-63 0.5237342077531864 1104 64-65 0.2099890964639073 1104 66-67 0.4912429616199532 1104 68-69 0.1834617488984449 1104 70-71 0.4257550019590397 1104 72-73 0.3526096605337088 1104 74-75 0.38064201391997443 1104 76-77 0.7584170294513761 1104 78-79 0.6899983012673587 1104 80-81 0.5850154870865012 1104 82-83 0.2928695599277056 1104 84-85 0.6278721487082812 1104 86-87 0.6848816286336188 1104 88-89 0.6497439388372541 1104 90-91 0.6188752931856101 1104 92-93 0.22971103690453276 1104 94-95 0.3366877443917424 1104 96-97 0.9272147784912619 1104 98-99 1.2811630665611737 1104 100-101 1.7596832029237923 1105 1 0.11178058027518745 1105 2 0.09297433816183798 1105 3 -0.057720925182088934 1105 4 -0.23252814500538221 1105 5 0.0745486551287513 1105 6 -0.039875543843038486 1105 7 -0.09518330667972208 1105 8 0.07555032878180157 1105 9 -0.009957512462783313 1105 10-11 0.037331990231997736 1105 12-13 0.0676799140264066 1105 14-15 0.0037508186164316726 1105 16-17 -0.13215660975924237 1105 18-19 -0.035934695846407294 1105 20-21 0.165695768628062 1105 22-23 0.04974983737405836 1105 24-25 -0.3200692939372445 1105 26-27 -0.12133444473294475 1105 28-29 -0.18097901064207633 1105 30-31 0.18187935205139638 1105 32-33 0.2857272679342415 1105 34-35 0.4468962674077446 1105 36-37 0.134999356382842 1105 38-39 -0.023514445945323814 1105 40-41 0.036110281000013345 1105 42-43 0.08080239781801879 1105 44-45 -0.050372286870192795 1105 46-47 -0.4992440094571151 1105 48-49 -0.13918108510121385 1105 50-51 0.22473090667349993 1105 52-53 0.2652116553691002 1105 54-55 -0.0188239745885852 1105 56-57 -0.10192744200715254 1105 58-59 0.03223746383114445 1105 60-61 -0.3550673092723642 1105 62-63 -0.7004805978333337 1105 64-65 -0.45781957182013855 1105 66-67 -0.5769165033331589 1105 68-69 -0.32125925300307046 1105 70-71 -0.4362048695247971 1105 72-73 -0.40866828845323866 1105 74-75 -0.355555612465178 1105 76-77 -0.4880913962256308 1105 78-79 -0.3098705597909337 1105 80-81 -0.3090078556451239 1105 82-83 -0.4949804171229779 1105 84-85 -0.07231144581010795 1105 86-87 0.0641846251584397 1105 88-89 0.3373218025838902 1105 90-91 0.06904249533628715 1105 92-93 0.3577682134831157 1105 94-95 0.09393317092115083 1105 96-97 0.5030622639020557 1105 98-99 1.1573187284849844 1105 100-101 1.4498838455046084 1106 1 -0.46065319455924936 1106 2 -0.30885437947814154 1106 3 -0.578002382135729 1106 4 -0.22496493007461993 1106 5 -0.29168403602115944 1106 6 -0.4648830694300372 1106 7 -0.5032206335912193 1106 8 -0.09941956887021774 1106 9 -0.3547799086096788 1106 10-11 -0.47512285624603123 1106 12-13 -0.49882746104884745 1106 14-15 -0.756258212087964 1106 16-17 -0.6588536296267975 1106 18-19 -0.816319253341895 1106 20-21 -0.438928704580853 1106 22-23 -1.0943275256602618 1106 24-25 -0.8887214613062966 1106 26-27 -0.7050942279960353 1106 28-29 -1.0939267830683264 1106 30-31 -0.8643089381352453 1106 32-33 -0.7406687284534712 1106 34-35 -1.1694493075470973 1106 36-37 -0.763066567759239 1106 38-39 -1.0478409059091973 1106 40-41 -1.5446768953997392 1106 42-43 -1.180147331260855 1106 44-45 -1.687845947315715 1106 46-47 -1.3540001804384545 1106 48-49 -2.38372503452927 1106 50-51 -2.5434433859213215 1106 52-53 -2.495587561969849 1106 54-55 -2.2431805369004394 1106 56-57 -2.2958618188885467 1106 58-59 -1.6987604290044942 1106 60-61 -2.9381309757383463 1106 62-63 -2.63271268693628 1106 64-65 -2.9583839908448226 1106 66-67 -3.001303167993008 1106 68-69 -3.4646593132077648 1106 70-71 -3.0492956281039696 1106 72-73 -2.380654290861429 1106 74-75 -3.0461975450359198 1106 76-77 -3.0801293251840853 1106 78-79 -2.9035829318559543 1106 80-81 -3.1273213715993684 1106 82-83 -3.373028279856271 1106 84-85 -3.2007732158281677 1106 86-87 -3.1373505945887032 1106 88-89 -3.601043639920622 1106 90-91 -4.232187938137521 1106 92-93 -4.148101744373594 1106 94-95 -3.8457492493076266 1106 96-97 -4.285543997386323 1106 98-99 -4.75818774351983 1106 100-101 -4.612729038300326 1107 1 0.052228484830067146 1107 2 0.11112306673976491 1107 3 -0.13668558824259947 1107 4 0.02499169726750239 1107 5 0.007110204089876504 1107 6 0.31005620884893403 1107 7 0.265516354611421 1107 8 0.3170446920596959 1107 9 0.09959205530427084 1107 10-11 0.2583521611030264 1107 12-13 0.0780939476950806 1107 14-15 -0.0679341315883093 1107 16-17 -0.0126227135962651 1107 18-19 0.08832595831667334 1107 20-21 -0.389028461693961 1107 22-23 -0.06027998922723299 1107 24-25 -0.19360955984897288 1107 26-27 0.10380584140021654 1107 28-29 -0.1081313285228731 1107 30-31 -0.09290453147320221 1107 32-33 -0.3171997832765143 1107 34-35 -0.2532452825644427 1107 36-37 -0.29364429156215266 1107 38-39 0.035456283542572464 1107 40-41 -0.4694253339840557 1107 42-43 -0.5433065956605674 1107 44-45 -0.8171181541165424 1107 46-47 -1.7316892852267927 1107 48-49 -1.8320681989983925 1107 50-51 -1.976345884186415 1107 52-53 -1.7697461324070503 1107 54-55 -1.3463857763175184 1107 56-57 -1.4293628598323451 1107 58-59 -1.3813159460065734 1107 60-61 -1.7772401013455692 1107 62-63 -2.0611044287822224 1107 64-65 -2.285457203891319 1107 66-67 -2.4169650347522023 1107 68-69 -2.0014826997448907 1107 70-71 -2.565798924425966 1107 72-73 -2.3807627225061196 1107 74-75 -2.5911099322670097 1107 76-77 -2.47161093517715 1107 78-79 -3.086246880502724 1107 80-81 -3.547797603382854 1107 82-83 -3.2191854623684115 1107 84-85 -3.378514367805966 1107 86-87 -3.3398141615560846 1107 88-89 -2.9968625157013307 1107 90-91 -3.232383115097967 1107 92-93 -3.2488998012785224 1107 94-95 -3.151049388100134 1107 96-97 -2.6523899377055464 1107 98-99 -3.273953704657931 1107 100-101 -2.4652620015203155 1108 1 0.18646219005613318 1108 2 0.09333093520717739 1108 3 0.2210360794027295 1108 4 0.27302458041489075 1108 5 0.19319358635646466 1108 6 0.30653301448604964 1108 7 0.13069545032486474 1108 8 0.07453148008083588 1108 9 0.330185126355353 1108 10-11 -0.10650397512714704 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40-41 -0.09148036171759344 2316 42-43 -0.37247074525692625 2316 44-45 -0.5436544823059037 2316 46-47 -0.052986445971484386 2316 48-49 0.045272676392905 2316 50-51 -0.08501467434512477 2316 52-53 -0.043045050524455064 2316 54-55 -0.3303085617532844 2316 56-57 -0.28204559913707783 2316 58-59 0.08651589539053361 2316 60-61 0.3134018888790564 2316 62-63 0.06034650207614334 2316 64-65 0.04468173838996847 2316 66-67 0.37396252854068024 2316 68-69 0.1361827404220577 2316 70-71 0.5056218015886458 2316 72-73 0.4887660819312458 2316 74-75 0.1709582757619863 2316 76-77 0.35683928174854884 2316 78-79 0.33384757828138945 2316 80-81 0.2815038409578605 2316 82-83 0.35845471556557484 2316 84-85 0.17684287311885427 2316 86-87 0.5220673256205828 2316 88-89 0.4472722528197295 2316 90-91 0.27989103504756585 2316 92-93 0.5186739259991988 2316 94-95 0.2726696519350149 2316 96-97 0.13278497579876358 2316 98-99 0.17855604326891594 2316 100-101 0.7219668413595777 >>END_MODULE >>Per sequence quality scores pass #Quality Count 3 1.0 4 1.0 5 9.0 6 7.0 7 32.0 8 77.0 9 164.0 10 247.0 11 461.0 12 773.0 13 1129.0 14 1520.0 15 2279.0 16 3327.0 17 4712.0 18 4340.0 19 2438.0 20 2013.0 21 1926.0 22 1989.0 23 1995.0 24 2105.0 25 2320.0 26 2806.0 27 3153.0 28 3550.0 29 4336.0 30 5077.0 31 6405.0 32 8111.0 33 11079.0 34 15888.0 35 24082.0 36 40242.0 37 68911.0 38 65225.0 39 6515.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.2576568142303 20.57275645312291 15.507222147920288 20.662364584726493 2 15.013784691473543 21.58699393473575 40.971444802753595 22.42777657103711 3 19.76307039382446 26.089993149426054 30.597336630520143 23.549599826229343 4 12.48809503918194 18.071814065397916 38.10890741699945 31.331183478420694 5 12.847436895734676 38.9005671279664 33.838858124433955 14.41313785186497 6 30.49541345720062 37.9431569449782 17.275476616150645 14.285952981670539 7 27.599792811909975 34.715032832628786 20.486892011562432 17.198282343898814 8 26.30887734130896 35.43551270697923 19.453290781800867 18.80231916991094 9 26.815151464519037 17.595615632675567 21.14087119250113 34.44836171030426 10-11 24.349780280372272 28.455279119116444 26.949489548697553 20.24545105181373 12-13 25.840965562646108 25.795681837550067 28.129715314086916 20.233637285716913 14-15 22.33253688449264 26.798944009089542 27.598623201724337 23.269895904693477 16-17 20.599675850891412 30.284549449447777 27.975237681498438 21.140537018162377 18-19 20.297749335828502 28.270814884125052 31.739377433206904 19.69205834683955 20-21 21.613596908213854 27.215537796016303 31.584039138563952 19.5868261572059 22-23 21.02704030397158 27.614427956295728 31.397029459778736 19.96150227995395 24-25 20.913966816488163 27.500543033300474 31.834282945412625 19.751207204798742 26-27 20.73351880164081 27.810053718973744 31.775065373401173 19.681362105984277 28-29 20.171835890375252 28.164777986826582 31.982796360100117 19.680589762698045 30-31 20.54256636295738 27.738020247657012 31.722554633418493 19.99685875596711 32-33 20.393323196711723 28.09219870006182 31.466524085615465 20.047954017610987 34-35 20.69458136309713 27.793613928386442 31.6929272001203 19.81887750839613 36-37 21.21893635471997 27.44260963860656 31.587715062432224 19.75073894424125 38-39 20.761416230847633 28.0367257598289 31.53870574278601 19.663152266537452 40-41 20.639246364994335 28.468836363757884 31.182517218447884 19.7094000527999 42-43 20.877275082312437 28.30895993849548 30.77432186251734 20.039443116674747 44-45 21.02173804073585 28.301558923290283 30.449464485622148 20.227238550351718 46-47 21.005697672475733 28.94885461745392 29.969088873665388 20.076358836404953 48-49 21.405704355962506 29.199819545857075 29.346187906230682 20.04828819194974 50-51 21.136192751758593 29.262310147203795 29.273504987552005 20.327992113485603 52-53 21.133853531387324 29.672342060853147 28.794131898611507 20.399672509148022 54-55 20.763087102541398 29.48353355945797 28.949355878962052 20.804023459038582 56-57 21.23477418169059 29.41519490718308 29.128306237364033 20.2217246737623 58-59 21.35591237948838 28.977092349078514 28.956540627245236 20.710454644187873 60-61 20.965763838994803 29.035572858360208 29.763905829671337 20.234757472973648 62-63 20.891242961452992 28.816354492138547 29.888719945195408 20.403682601213053 64-65 20.79733997226353 28.89755885645541 29.69189125967017 20.613209911610888 66-67 20.5652558939999 28.912763788868652 29.516115557486337 21.005864759645107 68-69 20.616384567829034 28.783438319771427 29.52614078764892 21.074036324750622 70-71 20.700095239686544 29.250614045347454 29.172083075740613 20.877207639225386 72-73 20.552223094788552 28.51426088990626 29.55153803739411 21.381977977911077 74-75 20.536851075205934 28.83724038831058 29.11176460759578 21.514143928887698 76-77 20.34486791759261 28.291032431619577 29.52045982389012 21.843639826897693 78-79 20.405854734414945 28.080335511036107 29.06865611789671 22.44515363665224 80-81 20.380290397500374 28.305234841016556 29.32129191799362 21.993182843489446 82-83 20.326655416130595 28.45444368326956 29.34836003943257 21.87054086116727 84-85 19.90128473539196 28.641127907112747 28.911308311430954 22.54627904606434 86-87 19.883540242944743 28.314257548162875 28.590285551972467 23.211916656919914 88-89 19.87167705391903 28.09186452572307 28.951862186502698 23.084596233855205 90-91 19.835263172846666 27.32679488443523 29.29200596916156 23.54593597355655 92-93 20.03495527829715 27.266453793700364 29.07286638294744 23.62572454505505 94-95 19.07383582014737 28.100720145700013 29.05428662133035 23.77115741282227 96-97 19.008671824090627 28.40314792227105 29.54017610987652 23.048004143761798 98-99 19.022491942100082 29.582225667982776 28.027623266235686 23.367659123681452 100-101 18.81346131992861 30.53228965434261 26.771402215255453 23.882846810473332 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 135.0 1 94.5 2 56.5 3 72.5 4 74.5 5 73.0 6 114.0 7 221.5 8 388.0 9 508.0 10 526.5 11 538.5 12 611.5 13 675.0 14 719.0 15 759.0 16 797.0 17 832.0 18 880.5 19 934.5 20 986.0 21 1130.0 22 1309.0 23 1460.0 24 1722.0 25 2068.5 26 2366.5 27 2846.5 28 3346.5 29 3881.5 30 4507.0 31 5144.0 32 5727.0 33 6305.5 34 7116.0 35 7820.0 36 8720.0 37 9318.5 38 9597.5 39 9877.0 40 10126.0 41 10689.0 42 10971.5 43 11381.5 44 11950.0 45 11987.0 46 11901.0 47 12401.0 48 13197.0 49 12246.5 50 10577.0 51 9465.0 52 8475.5 53 7859.5 54 7161.5 55 6431.5 56 5544.5 57 4702.0 58 4140.0 59 3580.0 60 2831.5 61 2113.0 62 1533.0 63 1101.0 64 774.5 65 523.0 66 393.5 67 322.5 68 240.5 69 135.0 70 75.5 71 53.0 72 38.5 73 34.0 74 34.0 75 19.5 76 9.0 77 7.5 78 7.5 79 4.0 80 4.0 81 5.0 82 3.5 83 1.5 84 0.5 85 0.0 86 0.5 87 0.5 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.05513876589416698 2 0.0 3 0.0 4 0.0 5 0.006015138097545489 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.006850573944426807 14-15 0.0 16-17 0.0 18-19 0.0 20-21 1.670871693762636E-4 22-23 0.002172133201891427 24-25 0.0 26-27 8.354358468813179E-4 28-29 0.002005046032515163 30-31 1.670871693762636E-4 32-33 0.0 34-35 0.0 36-37 1.670871693762636E-4 38-39 0.0 40-41 6.683486775050544E-4 42-43 0.026733947100202174 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 0.0 84-85 1.670871693762636E-4 86-87 0.0 88-89 0.0 90-91 0.013534060719477351 92-93 0.0018379588631388993 94-95 0.0 96-97 0.0 98-99 0.0015037845243863722 100-101 0.01570619392136878 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 299245.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 69.88505553957152 #Duplication Level Percentage of deduplicated Percentage of total 1 86.635047046159 60.54495074494214 2 8.087864050494657 11.304416567306461 3 2.2108623645911636 4.635187174194057 4 0.9320730157631865 2.6055189789418485 5 0.5344304629725005 1.867435129343606 6 0.35491622714094817 1.488200414738421 7 0.23633360968157544 1.156133120692502 8 0.16163775477351564 0.9036850775711026 9 0.11694031841282121 0.7355142582385671 >10 0.669839775458997 8.418507202486895 >50 0.035072338738179824 1.6673735073315936 >100 0.023061263827843567 2.638385223643045 >500 4.804429964134077E-4 0.3159481414416016 >1k 0.001441328989240223 1.7187444591281174 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2219 0.7415328576918578 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1675 0.5597420174104831 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1225 0.4093635649718458 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 941 0.3144580527661281 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 429 0.14336079132483417 No Hit GCTAAGGGTAGTTTATTTTGGACACAGACATATTAGGGCATTGTCACCAC 416 0.13901652492105132 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 311 0.10392821935203596 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 3.341743387525272E-4 0.0 14-15 0.0 0.0 0.0 3.341743387525272E-4 0.0 16-17 0.0 0.0 0.0 3.341743387525272E-4 0.0 18-19 0.0 0.0 0.0 3.341743387525272E-4 0.0 20-21 0.0 0.0 0.0 3.341743387525272E-4 0.0 22-23 0.0 0.0 0.0 3.341743387525272E-4 0.0 24-25 0.0 0.0 0.0 0.0010025230162575816 0.0 26-27 0.0 0.0 0.0 0.0015037845243863724 0.0 28-29 0.0 0.0 0.0 0.005346789420040435 0.0 30-31 0.0 0.0 0.0 0.015037845243863724 0.0 32-33 0.0 0.0 0.0 0.02639977276144965 0.0 34-35 0.0 0.0 0.0 0.036425002924025465 0.0 36-37 0.0 0.0 0.0 0.05046032515163161 0.0 38-39 0.0 0.0 0.0 0.06633360624237665 0.0 40-41 0.0 0.0 0.0 0.08604989222877575 0.0 42-43 0.0 0.0 0.0 0.131831776637872 0.0 44-45 0.0 0.0 0.0 0.18814015271767282 0.0 46-47 0.0 0.0 0.0 0.24845862086250398 0.0 48-49 0.0 0.0 0.0 0.32064027803304984 0.0 50-51 0.0 0.0 0.0 0.41136861100436095 0.0 52-53 0.0 0.0 0.0 0.5985062407057762 0.0 54-55 0.0 0.0 0.0 0.7458771240956408 0.0 56-57 0.0 0.0 0.0 1.052147905562332 0.0 58-59 0.0 0.0 0.0 1.4444685792577987 0.0 60-61 0.0 0.0 0.0 1.8352854684288793 0.0 62-63 0.0 0.0 0.0 2.4202576484151783 0.0 64-65 0.0 0.0 0.0 2.741566275125733 0.0 66-67 0.0 0.0 0.0 3.3323865060402014 0.0 68-69 0.0 0.0 0.0 3.959798827048071 0.0 70-71 0.0 0.0 0.0 4.549616534946281 0.0 72-73 0.0 0.0 0.0 5.5701849654965 0.0 74-75 0.0 0.0 0.0 6.483817607645909 0.0 76-77 0.0 0.0 0.0 7.162191515313539 0.0 78-79 0.0 0.0 0.0 7.919764741265519 0.0 80-81 0.0 0.0 0.0 9.204330899430232 0.0 82-83 0.0 0.0 0.0 9.98880515965179 0.0 84-85 0.0 0.0 0.0 11.039115106350984 0.0 86-87 0.0 0.0 0.0 12.805560660996843 0.0 88-89 0.0 0.0 0.0 15.104345937275477 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TAGTCGG 25 0.0016110261 38.020004 40-41 TATAGAC 65 1.3269494E-4 36.533257 3 AGGGTAG 145 6.6029315E-10 32.753952 5 TTAGTCG 30 0.0039392896 31.683332 38-39 GACCGCG 60 0.0039458456 31.667448 7 CTGGTCG 60 0.0039458456 31.667448 9 CTAAGGG 150 9.513315E-10 31.662155 2 TAAGGGT 170 1.07320375E-10 30.730915 3 GCTTATA 140 1.688386E-8 30.551786 1 ATAGGAG 125 2.1475898E-7 30.395668 5 AAGGGTA 175 1.5097612E-10 29.852888 4 GTAAGGT 80 4.4895307E-4 29.683271 4 GCTAAGG 165 2.6157068E-9 28.80303 1 CTGAACG 85 6.395098E-4 27.941864 9 GGGTAGT 190 3.929017E-10 27.496082 6 GTATCAA 3005 0.0 26.886024 1 TAAGGTG 125 7.3751835E-6 26.59621 5 CGTGTGA 45 8.9150807E-4 26.402777 38-39 GAACGTT 45 8.941229E-4 26.389538 18-19 TATAGGC 90 8.939459E-4 26.385128 3 >>END_MODULE