FastQCFastQC Report
Thu 26 May 2016
SRR937688_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937688_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences191946
Sequences flagged as poor quality0
Sequence length101
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17460.9096308336719702No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12320.641847186187782No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8570.44647973909328664No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3110.16202473612370144No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT2700.14066456190803664No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN2640.13753868275452472No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT2310.12034634741020912No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT2260.11774144811561585No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC2150.11201066966751065No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2070.10784283079616143No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATTGGCG250.005010700357.012077
GGTATCA9850.038.607021
GACATAC801.0675378E-535.651171
GTATCAA15500.034.6544721
CTAGACT953.470802E-530.0063514
TATCAAC17950.029.1147982
ATCAACG18150.028.7939743
TCAACGC18400.028.6609574
CAACGCA18500.028.5060355
AACGCAG19000.027.7486276
GTGTACA1056.841997E-527.1627961
CCTAGAC700.00836576727.1486073
TACATGG8150.026.2325482
GTACATG8050.025.9818061
ACGCAGA20400.025.851067
GAGTACT12750.025.71132512-13
CGCAGAG20700.025.4764088
ACATGGG8150.025.0666563
TAGACTG1151.2744736E-424.7878555
GTACTTT13650.024.17747114-15
Produced by FastQC (version 0.11.5)