##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937688_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 191946 Sequences flagged as poor quality 0 Sequence length 101 %GC 41 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.04775301386849 33.0 31.0 34.0 31.0 34.0 2 32.151089368884996 33.0 31.0 34.0 31.0 34.0 3 32.18670876183927 34.0 31.0 34.0 30.0 34.0 4 35.75792670855345 37.0 35.0 37.0 35.0 37.0 5 35.76600189636669 37.0 35.0 37.0 35.0 37.0 6 35.842742229585404 37.0 36.0 37.0 35.0 37.0 7 35.77705187917435 37.0 36.0 37.0 35.0 37.0 8 35.785033290612986 37.0 36.0 37.0 35.0 37.0 9 37.37358423723339 39.0 37.0 39.0 35.0 39.0 10-11 37.350955477061255 39.0 37.0 39.0 35.0 39.0 12-13 37.27169881112396 39.0 37.0 39.0 35.0 39.0 14-15 38.450147437300075 40.0 38.0 41.0 34.0 41.0 16-17 38.39878663790858 40.0 38.0 41.0 34.0 41.0 18-19 38.39618173861399 40.0 38.0 41.0 34.0 41.0 20-21 38.438464463963825 40.0 38.0 41.0 34.0 41.0 22-23 38.428841965969596 40.0 38.0 41.0 34.0 41.0 24-25 38.35624081772998 40.0 38.0 41.0 34.0 41.0 26-27 38.26012524355808 40.0 38.0 41.0 34.0 41.0 28-29 38.11010388338387 40.0 38.0 41.0 34.0 41.0 30-31 37.83200743959239 40.0 37.5 41.0 33.5 41.0 32-33 37.64325383180686 40.0 37.0 41.0 33.0 41.0 34-35 37.5720775634814 40.0 37.0 41.0 33.0 41.0 36-37 37.409474539714296 40.0 37.0 41.0 33.0 41.0 38-39 37.23133329165495 40.0 37.0 41.0 33.0 41.0 40-41 36.971648276077644 40.0 36.5 41.0 31.5 41.0 42-43 36.640440540568704 40.0 36.0 41.0 31.0 41.0 44-45 36.27093036583206 40.0 36.0 41.0 30.0 41.0 46-47 35.734612859866836 39.0 35.5 41.0 28.0 41.0 48-49 35.25177393641962 39.0 35.0 41.0 25.0 41.0 50-51 34.336894230669046 38.5 34.0 40.0 23.5 40.5 52-53 34.39701270148896 38.5 35.0 40.0 23.0 40.5 54-55 34.62404530440853 39.0 35.0 40.0 22.5 41.0 56-57 34.397145551352985 39.0 35.0 40.0 19.5 41.0 58-59 34.13540006043367 38.5 34.5 40.0 18.0 41.0 60-61 33.79651568670355 38.0 34.0 40.0 12.5 41.0 62-63 33.453559855375985 38.0 34.0 40.0 7.5 41.0 64-65 33.02027132631052 37.5 33.0 40.0 4.0 41.0 66-67 32.45894418221792 37.0 32.5 40.0 2.0 41.0 68-69 32.04773217467413 36.0 32.0 39.0 2.0 41.0 70-71 31.558836860366977 36.0 31.0 39.0 2.0 40.0 72-73 31.092770883477645 35.0 31.0 38.5 2.0 40.0 74-75 30.56758150729893 35.0 30.5 38.0 2.0 40.0 76-77 30.061217738322235 35.0 30.0 37.0 2.0 39.0 78-79 29.565906557052504 34.5 29.0 36.5 2.0 39.0 80-81 29.08245808717035 34.0 29.0 36.0 2.0 38.5 82-83 28.577633813676762 34.0 29.0 36.0 2.0 37.0 84-85 28.081562001813012 34.0 27.5 35.0 2.0 37.0 86-87 27.57198378710679 34.0 26.0 35.0 2.0 36.5 88-89 27.105610432100693 34.0 26.0 35.0 2.0 36.0 90-91 26.614850009898618 33.0 25.0 35.0 2.0 35.5 92-93 26.03536932262199 33.0 24.0 35.0 2.0 35.0 94-95 25.38280037093766 32.0 19.5 34.5 2.0 35.0 96-97 24.651842184781138 32.0 12.5 34.0 2.0 35.0 98-99 23.563484000708534 31.5 2.0 34.0 2.0 35.0 100-101 20.451082596146833 28.0 2.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.2628136509768453 1101 2 0.2645724373799041 1101 3 0.19690290236283658 1101 4 0.39665616724598607 1101 5 -0.0738840071190836 1101 6 0.051337011312199365 1101 7 0.22322644636989963 1101 8 0.07927271492707888 1101 9 0.1935854984538068 1101 10-11 0.27291091587864713 1101 12-13 0.3962372633730027 1101 14-15 0.5179167881743112 1101 16-17 0.5965874981861887 1101 18-19 0.5269872072486166 1101 20-21 0.47687466637385256 1101 22-23 0.36065315016077903 1101 24-25 0.10128754681723251 1101 26-27 0.5460365479433946 1101 28-29 0.49738791448821473 1101 30-31 0.48738312616313095 1101 32-33 0.6208275464288349 1101 34-35 0.5585849559483904 1101 36-37 0.8705102251936978 1101 38-39 1.090599220264977 1101 40-41 0.8238150522106693 1101 42-43 1.0106265853170555 1101 44-45 1.2366562121275422 1101 46-47 1.709081162158597 1101 48-49 2.169823485268232 1101 50-51 1.8799451707847936 1101 52-53 1.91217370440895 1101 54-55 2.525317950259357 1101 56-57 2.74037943301807 1101 58-59 2.2055066833324446 1101 60-61 2.6755668851470134 1101 62-63 2.407507498181154 1101 64-65 2.383423329473139 1101 66-67 2.485954435596785 1101 68-69 1.9604482265964833 1101 70-71 2.2895008562798758 1101 72-73 2.27409636116575 1101 74-75 2.5358163390097452 1101 76-77 2.3637965489758592 1101 78-79 2.590714897842929 1101 80-81 2.8994891390744435 1101 82-83 2.2765063883701515 1101 84-85 2.2036864035686143 1101 86-87 2.5719861902162613 1101 88-89 2.8379409606861543 1101 90-91 2.5291456380995143 1101 92-93 2.308674278338433 1101 94-95 2.4925580926589035 1101 96-97 2.695217019642538 1101 98-99 3.079168956899469 1101 100-101 3.545943688336081 1102 1 0.5665617700776622 1102 2 0.5154957878883195 1102 3 0.4548938740467534 1102 4 0.4662836352136992 1102 5 0.4522889900082703 1102 6 0.44023856618886015 1102 7 0.387286452753564 1102 8 0.6316643067377612 1102 9 0.4167855349318472 1102 10-11 0.542027691219495 1102 12-13 0.6171346232745023 1102 14-15 0.48261288046376194 1102 16-17 0.6930377284538451 1102 18-19 0.6333663053289627 1102 20-21 1.0593150475694202 1102 22-23 0.46496895883343825 1102 24-25 0.7245377406068414 1102 26-27 0.7638332740388734 1102 28-29 0.8803275889216664 1102 30-31 0.8929505877471868 1102 32-33 0.8629733674128275 1102 34-35 0.9425391760021995 1102 36-37 0.740602788232323 1102 38-39 1.165390611446412 1102 40-41 0.9687012863085158 1102 42-43 1.0175460144356592 1102 44-45 1.111730308155984 1102 46-47 0.997383109174244 1102 48-49 1.0826067600847082 1102 50-51 1.2992602954941077 1102 52-53 1.1269495924213615 1102 54-55 1.8719277719729135 1102 56-57 1.5422466529350842 1102 58-59 1.6979260896522632 1102 60-61 1.4558208635337806 1102 62-63 1.537631523533392 1102 64-65 1.4507481208643256 1102 66-67 1.2017334698405513 1102 68-69 1.6308690920380897 1102 70-71 1.7475055072305885 1102 72-73 2.127317828495098 1102 74-75 1.8692826652146621 1102 76-77 1.5197173916186948 1102 78-79 1.8266138536588983 1102 80-81 2.1058066804542257 1102 82-83 1.9668533857710884 1102 84-85 1.7131706952105823 1102 86-87 2.095890708934057 1102 88-89 2.1206686064843367 1102 90-91 2.3775565632739237 1102 92-93 2.9740110162892783 1102 94-95 2.6717450882359373 1102 96-97 2.0138413413788925 1102 98-99 2.9132394507209973 1102 100-101 2.991648398563612 1103 1 0.45270164176666583 1103 2 0.32271169337011685 1103 3 0.3996947379227578 1103 4 0.17256579236291003 1103 5 0.209736744565852 1103 6 0.31008548227854504 1103 7 0.43175065906747534 1103 8 0.1687601588625114 1103 9 0.3331293067669492 1103 10-11 0.23897236504277686 1103 12-13 0.33577147774951044 1103 14-15 0.4117718717614878 1103 16-17 0.6674866898970322 1103 18-19 0.3718532519083979 1103 20-21 0.4576508158473871 1103 22-23 0.33111581775486343 1103 24-25 0.3563423521802349 1103 26-27 0.27053466513334 1103 28-29 0.2961790784725764 1103 30-31 0.6154621802009856 1103 32-33 0.8055827811205063 1103 34-35 0.42821534953964147 1103 36-37 0.9311319122874551 1103 38-39 0.9241564121197285 1103 40-41 0.6389039160487258 1103 42-43 0.8105810141180498 1103 44-45 0.9277914146795254 1103 46-47 1.4072319308727756 1103 48-49 1.6860972002803507 1103 50-51 1.6356955365536265 1103 52-53 2.104352247569672 1103 54-55 2.268008569787817 1103 56-57 2.708431624033345 1103 58-59 2.9514832261573787 1103 60-61 2.948754230744605 1103 62-63 2.564548248426995 1103 64-65 2.239670181462685 1103 66-67 2.5374858677316787 1103 68-69 3.1581192984790505 1103 70-71 3.1420300578245026 1103 72-73 3.1066102124117556 1103 74-75 3.1833111223067014 1103 76-77 3.3947729718765878 1103 78-79 2.823151997595886 1103 80-81 2.8306526437394943 1103 82-83 2.889295105900672 1103 84-85 2.9580096712634294 1103 86-87 2.382541918995674 1103 88-89 2.717537055953944 1103 90-91 2.7040191180601774 1103 92-93 2.599622399126094 1103 94-95 2.8983696197737636 1103 96-97 2.929692830929806 1103 98-99 2.935439793730591 1103 100-101 3.2393934281005485 1104 1 0.503173938397147 1104 2 0.2551098323804126 1104 3 0.4177054527119921 1104 4 0.3344155033455678 1104 5 0.45885559413585497 1104 6 0.2632082794024697 1104 7 0.2676804490012188 1104 8 0.4355372630497385 1104 9 0.5451693462424885 1104 10-11 0.4270451128222774 1104 12-13 0.4442587186912874 1104 14-15 0.6898629474699334 1104 16-17 0.8190093178400701 1104 18-19 0.3621253511611755 1104 20-21 0.3173892903997668 1104 22-23 0.3685470880213231 1104 24-25 0.42944259185316014 1104 26-27 0.527971574762546 1104 28-29 0.37352646321809857 1104 30-31 0.6489811425582346 1104 32-33 0.6717712768204933 1104 34-35 0.817807200660468 1104 36-37 0.9931825255681588 1104 38-39 1.0527867518913325 1104 40-41 0.556445858809191 1104 42-43 0.32480278205827773 1104 44-45 0.3761031304556468 1104 46-47 0.6090153793430986 1104 48-49 0.8222878107413649 1104 50-51 0.5669834260221194 1104 52-53 0.7652503160879363 1104 54-55 0.5459762844360583 1104 56-57 0.8135881163497203 1104 58-59 1.1901868776447628 1104 60-61 1.340302235820026 1104 62-63 1.2224680284918534 1104 64-65 1.3712788096876949 1104 66-67 1.6478940166108522 1104 68-69 1.3743359909927975 1104 70-71 1.4548560834836017 1104 72-73 1.675093223831471 1104 74-75 2.2452207515186053 1104 76-77 2.539651725577418 1104 78-79 2.5205296939162025 1104 80-81 2.4246890176292446 1104 82-83 1.8581023806786199 1104 84-85 2.4248431690781764 1104 86-87 2.5451670423567343 1104 88-89 2.5994276523165567 1104 90-91 2.990093416664436 1104 92-93 3.0225569825181715 1104 94-95 2.293160258431598 1104 96-97 2.4064304595777664 1104 98-99 2.9570279792656287 1104 100-101 4.239939741367149 1105 1 -0.5486283495372355 1105 2 0.11405651919240967 1105 3 0.17277776007222911 1105 4 -0.01956679753939028 1105 5 0.1953114542696781 1105 6 0.26655384383156644 1105 7 0.26611558821986137 1105 8 0.03315219937609726 1105 9 0.018944437975711992 1105 10-11 0.3403842019653851 1105 12-13 0.24384861724514195 1105 14-15 0.01395101135941701 1105 16-17 0.11584721984741009 1105 18-19 0.29100512668460254 1105 20-21 0.2554154073866002 1105 22-23 -0.4748589124103617 1105 24-25 -0.1251656533443395 1105 26-27 -0.8462999555633814 1105 28-29 -1.0218167269394698 1105 30-31 -0.2150918123306056 1105 32-33 0.13920598435340992 1105 34-35 0.15789461702135554 1105 36-37 -0.12188870257774909 1105 38-39 -0.6764113918600145 1105 40-41 -0.554200590575654 1105 42-43 -0.2022585173157765 1105 44-45 0.2624888416578841 1105 46-47 0.12212783347648326 1105 48-49 0.06575856403296854 1105 50-51 3.68921381493692E-4 1105 52-53 -0.4952180629321461 1105 54-55 -0.37731855691943395 1105 56-57 -0.2697002497197829 1105 58-59 -0.2876082874750381 1105 60-61 -1.2937901449267883 1105 62-63 -1.0136910477006822 1105 64-65 -0.8601047430858841 1105 66-67 -0.5594500369181752 1105 68-69 0.07903596940850832 1105 70-71 -0.17192462016414467 1105 72-73 -1.8074774647072758 1105 74-75 -1.52299099033333 1105 76-77 -2.0013853993955983 1105 78-79 -2.078326338891916 1105 80-81 -1.4615617917815058 1105 82-83 -1.2355194626194574 1105 84-85 -1.2729228572546525 1105 86-87 -1.9289318136760727 1105 88-89 -1.500453634106929 1105 90-91 -1.5280643644708967 1105 92-93 -0.8466152600892123 1105 94-95 -0.2257799530944098 1105 96-97 -0.4325507620928555 1105 98-99 -1.0783972311811674 1105 100-101 -0.9514859007653769 1106 1 -0.7114602079443166 1106 2 -0.37432106192853354 1106 3 -0.5062782871254079 1106 4 -0.9322511633210979 1106 5 -0.9164289587096732 1106 6 -0.5779299319796394 1106 7 -0.4363032908361859 1106 8 -0.47917818410473245 1106 9 -0.3484891903428746 1106 10-11 -0.7370199278281291 1106 12-13 -1.0944404683005828 1106 14-15 -0.9623321744812898 1106 16-17 -1.1535923081761865 1106 18-19 -0.5242161122534483 1106 20-21 -0.4064724982181218 1106 22-23 -0.490965107100628 1106 24-25 -0.9734842374151356 1106 26-27 -1.0760934658878654 1106 28-29 -0.6732621546680804 1106 30-31 -0.9353278005311978 1106 32-33 -1.1760742516347875 1106 34-35 -1.1443472708842535 1106 36-37 -1.097345929716397 1106 38-39 -1.486440890385083 1106 40-41 -1.1340825964753591 1106 42-43 -1.2952378683482308 1106 44-45 -1.572555406129723 1106 46-47 -1.8541553523642236 1106 48-49 -2.3689317014537608 1106 50-51 -2.1277726715388567 1106 52-53 -1.996457000985238 1106 54-55 -1.7949586749135378 1106 56-57 -2.1010866803982466 1106 58-59 -2.0448944231682518 1106 60-61 -2.419453861740948 1106 62-63 -2.4331010771992077 1106 64-65 -2.1207537106375085 1106 66-67 -2.943284845177768 1106 68-69 -3.072940431771432 1106 70-71 -2.8183552986302196 1106 72-73 -3.0101913290140594 1106 74-75 -2.4714865655545672 1106 76-77 -2.707462095560789 1106 78-79 -2.482397135352091 1106 80-81 -1.920676836029294 1106 82-83 -1.7956374567197528 1106 84-85 -2.688734066694181 1106 86-87 -3.134182551139201 1106 88-89 -2.1235804777647473 1106 90-91 -2.5155569898396273 1106 92-93 -2.584963342685082 1106 94-95 -1.6778153513245009 1106 96-97 -2.873081735544183 1106 98-99 -3.140167142685595 1106 100-101 -2.4394505025352906 1107 1 -0.07396020794431735 1107 2 0.08651227140479989 1107 3 -0.1646116204587429 1107 4 0.03441550334557064 1107 5 0.2869043746236599 1107 6 0.4879034013536909 1107 7 0.010363375830486632 1107 8 0.1199884825619364 1107 9 -0.645989190342874 1107 10-11 -0.07493659449479395 1107 12-13 0.1713928650327503 1107 14-15 -0.12858217448129494 1107 16-17 -0.06025897484285281 1107 18-19 -0.0029661122534534456 1107 20-21 0.20519416844854277 1107 22-23 0.6365348928993697 1107 24-25 0.08401576258486898 1107 26-27 -0.11151013255452824 1107 28-29 -0.1653454880014138 1107 30-31 0.40050553280213563 1107 32-33 -0.9852409183014572 1107 34-35 -0.18768060421758292 1107 36-37 0.32473740361693615 1107 38-39 0.1427257762815799 1107 40-41 -0.09949926314202884 1107 42-43 0.18059546498510315 1107 44-45 0.7161945938702772 1107 46-47 0.46084464763577415 1107 48-49 1.4835682985462384 1107 50-51 1.125977328461147 1107 52-53 0.33354299901476026 1107 54-55 0.5354579917531339 1107 56-57 1.2984966529350785 1107 58-59 0.5080222434984165 1107 60-61 0.6992961382590508 1107 62-63 1.1910655894674633 1107 64-65 0.43591295602915636 1107 66-67 0.7033818214889074 1107 68-69 1.1174762348952356 1107 70-71 0.34997803470311695 1107 72-73 0.8218920043192703 1107 74-75 0.2510134344454329 1107 76-77 -0.2528787622274571 1107 78-79 0.22510286464790852 1107 80-81 0.7268231639707068 1107 82-83 1.2222792099469117 1107 84-85 0.3970992666391524 1107 86-87 0.6649841155274672 1107 88-89 0.5405861889019192 1107 90-91 1.1515263434937033 1107 92-93 1.594203323981585 1107 94-95 1.1446846486755007 1107 96-97 1.3681682644558144 1107 98-99 2.1823328573144067 1107 100-101 3.5467994974647112 1108 1 -0.16146020794431593 1108 2 -0.09309299175309249 1108 3 0.10512522164651728 1108 4 0.07125760860872532 1108 5 -0.45256930958687036 1108 6 -0.14564923022525278 1108 7 -0.044899782064256044 1108 8 -0.9030378332275362 1108 9 -0.21638392718497812 1108 10-11 -0.4805286997579543 1108 12-13 -0.3911071349672497 1108 14-15 -0.38154270079708397 1108 16-17 -0.6549958169481158 1108 18-19 -0.5407950596218782 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-1.2519710341397499 2210 58-59 -1.2111529265696106 2210 60-61 -0.9922429773872068 2210 62-63 -0.6770466554305017 2210 64-65 -0.5712299011136963 2210 66-67 -0.6489991308920509 2210 68-69 -0.4473622004789135 2210 70-71 -1.0141376115553875 2210 72-73 -0.6492389480616794 2210 74-75 -0.604683844466134 2210 76-77 -0.88242808195535 2210 78-79 -0.35287332582828057 2210 80-81 -0.6300645911313332 2210 82-83 -0.654549021345602 2210 84-85 -1.0249245428846585 2210 86-87 -0.7475158844725343 2210 88-89 -0.4771859199416184 2210 90-91 -1.16875426875119 2210 92-93 -0.8373442950660355 2210 94-95 -0.5824412016646363 2210 96-97 -0.9865511232992858 2210 98-99 -0.3230242855427363 2210 100-101 -0.9791783936917504 2211 1 0.5450615311861213 2211 2 0.3395919815497308 2211 3 0.25748982881661675 2211 4 0.44311115551948177 2211 5 0.3006724905656881 2211 6 0.42033093758557527 2211 7 0.5494938106130931 2211 8 0.5176334100981705 2211 9 0.003105012555671749 2211 10-11 0.4266032605776644 2211 12-13 0.6233856186559379 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1.3791782364344733 2211 82-83 1.2330581954541593 2211 84-85 1.9440195564942222 2211 86-87 1.636542086541958 2211 88-89 1.7320716961482958 2211 90-91 1.9838633000154466 2211 92-93 1.5353265123873818 2211 94-95 1.9821846486754993 2211 96-97 1.9405414528616127 2211 98-99 2.6966444515173045 2211 100-101 2.155766888769058 2212 1 -0.3130350110939233 2212 2 -0.3868145002487431 2212 3 -0.3073281558918133 2212 4 -0.14589945728435083 2212 5 -0.1827806647464243 2212 6 -0.08680132305575938 2212 7 -0.5715263879490422 2212 8 -0.6137713599577452 2212 9 -0.7494340722326385 2212 10-11 -0.9799070669357448 2212 12-13 -0.9048866625263017 2212 14-15 -0.9680014658198743 2212 16-17 -0.8468731480712037 2212 18-19 -1.063743671308572 2212 20-21 -0.6662625244648481 2212 22-23 -0.6619887291478719 2212 24-25 -0.5841928988324554 2212 26-27 -0.5089018648379948 2212 28-29 -0.6355718659541694 2212 30-31 -0.5635692703474646 2212 32-33 -0.6668354064904349 2212 34-35 -0.7943735176034039 2212 36-37 -0.10857952551692307 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0.43064505521358143 2213 2 0.3990122714047999 2213 3 0.5077568005938886 2213 4 -0.45505818086495964 2213 5 0.2053254272552394 2213 6 0.20171919082737588 2213 7 0.38931074425153867 2213 8 0.28117269308825144 2213 9 0.24414238860449444 2213 10-11 0.4536818265578333 2213 12-13 0.3773139176643312 2213 14-15 0.10661519393975993 2213 16-17 0.16868839357819354 2213 18-19 0.08946809827286728 2213 20-21 0.38019416844854703 2213 22-23 0.3490348928993754 2213 24-25 0.3175683941638141 2213 26-27 0.4546082884981075 2213 28-29 0.7244571435775313 2213 30-31 0.5485318485916153 2213 32-33 0.11673276590907022 2213 34-35 0.2754772905192624 2213 36-37 0.12440845624851704 2213 38-39 0.4960152499657937 2213 40-41 0.5639875789632285 2213 42-43 0.6207270439324688 2213 44-45 0.1684972254492223 2213 46-47 -0.18783956289053805 2213 48-49 -0.25524749092744514 2213 50-51 -0.16250951364411748 2213 52-53 -0.21908857993260966 2213 54-55 0.028550097016285747 2213 56-57 -0.11564808390702552 2213 58-59 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80-81 1.0350778809518424 2214 82-83 0.857066945795971 2214 84-85 0.5558256817334915 2214 86-87 0.5307860023199176 2214 88-89 1.0922371322981448 2214 90-91 0.516266909531442 2214 92-93 0.40198634284950785 2214 94-95 0.7298261581094607 2214 96-97 0.948710717286005 2214 98-99 1.8221913478804446 2214 100-101 0.4797004408609382 2215 1 0.21470801635474857 2215 2 0.3307879723393796 2215 3 0.18001454776555903 2215 4 0.10544354072874285 2215 5 0.07989502882926303 2215 6 -0.028334916403316868 2215 7 -0.0027207363190484557 2215 8 0.1953389498516529 2215 9 0.308333239563666 2215 10-11 0.13715452700052566 2215 12-13 0.10188351923835626 2215 14-15 -0.058313482892501156 2215 16-17 -0.06399729259986486 2215 18-19 -0.11832826178616074 2215 20-21 -0.38288994370098806 2215 22-23 -0.35096510710062745 2215 24-25 0.05492697753813047 2215 26-27 -0.00490966526480463 2215 28-29 0.06041385779298025 2215 30-31 -7.795139268367279E-4 2215 32-33 0.0889413246892019 2215 34-35 -0.2862787350587013 2215 36-37 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-2.652874544080259 2316 92-93 -1.9579416464326158 2316 94-95 -1.7042058838688803 2316 96-97 -2.283495936727615 2316 98-99 -3.0736582669459516 2316 100-101 -2.6032374847838113 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 839.0 3 126.0 4 124.0 5 128.0 6 162.0 7 171.0 8 183.0 9 233.0 10 316.0 11 440.0 12 673.0 13 1057.0 14 1107.0 15 1623.0 16 2243.0 17 3241.0 18 2787.0 19 1648.0 20 1508.0 21 1459.0 22 1611.0 23 1729.0 24 1827.0 25 2012.0 26 2204.0 27 2389.0 28 2761.0 29 3056.0 30 3883.0 31 4658.0 32 6199.0 33 8188.0 34 11661.0 35 17299.0 36 26566.0 37 39641.0 38 32779.0 39 3412.0 40 3.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.13262453958883 19.804080353177817 15.582142577257645 21.481152529975706 2 14.39447739994987 20.34683348650681 42.69728047455928 22.561408638984044 3 18.609545794011815 26.11540811690177 30.802655149182407 24.47239093990401 4 11.651783382944279 16.74082197503786 39.099691889915924 32.507702752101935 5 12.049696577223969 37.60301229358373 35.60856895165081 14.738722177541492 6 29.405254723038617 37.52433950543169 18.434336843094368 14.636068928435328 7 26.214002724752973 34.28073307338564 22.083548651456073 17.42171555040532 8 25.070991585409143 34.553066210093334 20.856910195644456 19.519032008853067 9 25.8481809591923 17.198536205933483 21.724082126990922 35.229200707883294 10-11 23.425431670168752 27.465577322144057 28.311559494133466 20.797431513553725 12-13 25.1125664631552 25.37776652475679 29.010955163962027 20.498711848125982 14-15 21.682341056983784 25.975375154329445 28.909005009018323 23.43327877966845 16-17 19.595033708041125 29.839511618377472 29.158042819521697 21.40741185405971 18-19 19.48408514369826 27.437076552076917 33.22128383668612 19.857554467538705 20-21 20.272940095677797 26.449455972815606 33.256777177217934 20.020826754288667 22-23 19.799029075533745 26.58140627446886 33.57597744949627 20.043587200501122 24-25 19.79115283530514 26.60569853660828 33.57188158193506 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19.678715907607984 28.31267127756753 31.310974814041497 20.697638000782984 52-53 19.948793702826002 28.731117351679213 30.63138773763167 20.688701207863115 54-55 19.655958094850334 28.40394518088169 30.952287740342584 20.987808983925397 56-57 19.78894023887748 28.214890568854074 30.75815025221224 21.23801894005621 58-59 20.124406083582727 27.839514064827885 30.983585598325945 21.052494253263443 60-61 19.90829782070319 27.41696829568081 31.664652906990398 21.010080976625602 62-63 19.71275285472726 27.411122476671608 31.86700206667641 21.009122601924723 64-65 19.617524557136445 27.270947942002362 31.973078488887964 21.138449011973233 66-67 19.409033854119095 27.202031299337428 31.65110920321605 21.737825643327426 68-69 19.244449895743898 27.140512172780678 31.787802949442966 21.827234982032458 70-71 19.496472909386522 27.13785013192337 31.598451890379643 21.767225068310466 72-73 19.183058818616715 26.629275944054847 31.62717499954327 22.56049023778516 74-75 19.107276153488783 26.420752619133374 31.97117517787139 22.500796049506448 76-77 19.207942498316605 26.324663190257702 31.883524640222994 22.583869671202702 78-79 18.9569588274595 25.78059754468014 31.77692291631869 23.485520711541675 80-81 18.923863485112236 25.661395683866306 32.30680095102136 23.107939880000107 82-83 19.01631558070106 26.062130733418233 32.329484957932316 22.59206872794839 84-85 18.76280319933195 25.745488707088892 31.566133009746743 23.925575083832413 86-87 18.622924979579267 25.52369718337426 31.839901458549175 24.013476378497295 88-89 18.785395516350444 24.777774877991494 31.8897617245609 24.547067881097163 90-91 18.778543877322004 23.85237302378504 32.99346002682798 24.375623072064972 92-93 18.603109652030042 24.091452371383685 32.54669958297885 24.758738393607416 94-95 17.51289170963903 24.524781310674545 32.869684127017265 25.092642852669155 96-97 17.27794466526951 24.615368554568732 33.74682573031039 24.359861049851368 98-99 18.057947866754358 26.8018018018018 31.328228576200768 23.812021755243077 100-101 17.948724639572053 26.911605453715183 29.97612368712897 25.163546219583793 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 283.0 1 168.0 2 60.5 3 68.0 4 56.5 5 57.0 6 98.5 7 200.5 8 360.0 9 440.5 10 414.0 11 380.5 12 390.5 13 443.0 14 505.5 15 547.0 16 548.5 17 577.0 18 628.0 19 649.0 20 750.5 21 873.5 22 936.5 23 1048.5 24 1209.0 25 1404.5 26 1721.5 27 2077.5 28 2342.0 29 2621.5 30 2971.5 31 3421.0 32 3897.0 33 4259.5 34 4693.5 35 5227.0 36 5724.5 37 6116.0 38 6317.0 39 6481.5 40 6671.5 41 6843.0 42 7078.0 43 7392.0 44 7577.5 45 7629.5 46 7657.0 47 7924.0 48 8324.0 49 7751.0 50 6576.5 51 5794.5 52 5207.0 53 4890.5 54 4460.5 55 3808.0 56 3213.0 57 2706.0 58 2330.5 59 1867.5 60 1478.0 61 1136.5 62 808.5 63 572.5 64 404.5 65 294.5 66 209.0 67 148.5 68 98.5 69 65.0 70 47.0 71 37.0 72 26.0 73 21.5 74 17.0 75 12.5 76 7.5 77 6.5 78 6.0 79 4.5 80 3.0 81 1.5 82 1.0 83 1.5 84 2.5 85 1.5 86 0.0 87 0.0 88 0.0 89 0.0 90 0.5 91 1.0 92 1.0 93 0.5 94 0.0 95 0.5 96 0.5 97 0.0 98 0.5 99 0.5 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.2818501036749919 2 0.23027309764204518 3 0.24433955383284883 4 0.23756681566690632 5 0.24173465453825554 6 0.20057724568368188 7 0.1922415679409834 8 0.19484646723557666 9 0.2026611651193565 10-11 0.20500557448449044 12-13 0.20448459462557178 14-15 0.20396361476665315 16-17 0.195106957165036 18-19 0.18963666864639014 20-21 0.1904181384347681 22-23 0.195888426953414 24-25 0.19302303772936139 26-27 0.195888426953414 28-29 0.20135871547205986 30-31 0.20031675575422253 32-33 0.19562793702395465 34-35 0.19927479603638523 36-37 0.194325487376658 38-39 0.1857293197045002 40-41 0.18729225928125617 42-43 0.19250205787044272 44-45 0.19093911829368676 46-47 0.195888426953414 48-49 0.19771185645962927 50-51 0.1932835276588207 52-53 0.19172058808206474 54-55 0.19302303772936139 56-57 0.1779146218207204 58-59 0.1643691454888354 60-61 0.18104050097423235 62-63 0.1740072728788305 64-65 0.18338491033936627 66-67 0.1794775613974764 68-69 0.18260344055098832 70-71 0.18625029956341888 72-73 0.1904181384347681 74-75 0.19536744709449536 76-77 0.1922415679409834 78-79 0.18859470892855282 80-81 0.18885519885801214 82-83 0.18234295062152897 84-85 0.1789565815385577 86-87 0.18312442040990695 88-89 0.18807372906963418 90-91 0.18494784991612223 92-93 0.1818219707626103 94-95 0.18234295062152897 96-97 0.19093911829368676 98-99 0.1875527492107155 100-101 0.17374678294937118 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 191946.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 69.39528235998412 #Duplication Level Percentage of deduplicated Percentage of total 1 85.49078854587347 59.326574103185855 2 8.881278541846193 12.326376642581693 3 2.3507533366579265 4.8939357466815485 4 0.9907240783845783 2.750063086413314 5 0.6107791650846142 2.1192596310321083 6 0.3642588885529759 1.5166709053960652 7 0.29561840064126066 1.436016566831504 8 0.17690447618146662 0.982106886028637 9 0.12846016462947454 0.8023076456825188 >10 0.6610303265685045 7.878513050583528 >50 0.032606689882501844 1.5468984902890281 >100 0.013833141162273509 1.7916869481938078 >500 9.88081511590965E-4 0.5876294353624545 >1k 0.00197616302318193 2.0419608617379104 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1746 0.9096308336719702 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1232 0.641847186187782 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 857 0.44647973909328664 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 311 0.16202473612370144 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 270 0.14066456190803664 No Hit NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 264 0.13753868275452472 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 231 0.12034634741020912 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 226 0.11774144811561585 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 215 0.11201066966751065 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 207 0.10784283079616143 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0010419597178373083 0.0 18-19 0.0 0.0 0.0 0.0015629395767559626 0.0 20-21 0.0 0.0 0.0 0.0015629395767559626 0.0 22-23 0.0 0.0 0.0 0.0015629395767559626 0.0 24-25 0.0 0.0 0.0 0.0015629395767559626 0.0 26-27 0.0 0.0 0.0 0.002083919435674617 0.0 28-29 2.604899294593271E-4 0.0 0.0 0.006772738165942505 0.0 30-31 5.209798589186542E-4 0.0 0.0 0.016671355485396933 0.0 32-33 5.209798589186542E-4 0.0 0.0 0.030477321746741268 0.0 34-35 5.209798589186542E-4 0.0 0.0 0.043762308149166956 0.0 36-37 5.209798589186542E-4 0.0 0.0 0.05939170391672658 0.0 38-39 5.209798589186542E-4 0.0 0.0 0.0817938378502287 0.0 40-41 5.209798589186542E-4 0.0 0.0 0.10341450199535286 0.0 42-43 5.209798589186542E-4 0.0 0.0 0.1586383670407302 0.0 44-45 5.209798589186542E-4 0.0 0.0 0.22219790982880602 0.0 46-47 5.209798589186542E-4 0.0 0.0 0.29018578141769036 0.0 48-49 5.209798589186542E-4 0.0 0.0 0.38031529701061756 0.0 50-51 5.209798589186542E-4 0.0 0.0 0.4832088191470518 0.0 52-53 5.209798589186542E-4 0.0 0.0 0.6921217425734321 0.0 54-55 5.209798589186542E-4 0.0 0.0 0.8606587269336168 0.0 56-57 5.209798589186542E-4 0.0 0.0 1.2328988361309952 0.0 58-59 5.209798589186542E-4 0.0 0.0 1.7194940243610182 0.0 60-61 5.209798589186542E-4 0.0 0.0 2.1941066758359122 0.0 62-63 5.209798589186542E-4 0.0 0.0 2.973753034707678 0.0 64-65 5.209798589186542E-4 0.0 0.0 3.384545653465037 0.0 66-67 5.209798589186542E-4 0.0 0.0 4.128504892000875 0.0 68-69 5.209798589186542E-4 0.0 0.0 4.956602377752076 0.0 70-71 5.209798589186542E-4 0.0 0.0 5.718274931491148 0.0 72-73 5.209798589186542E-4 0.0 0.0 7.09183832953018 0.0 74-75 5.209798589186542E-4 0.0 0.0 8.354172527690078 0.0 76-77 5.209798589186542E-4 0.0 0.0 9.26041699227908 0.0 78-79 5.209798589186542E-4 0.0 0.0 10.188803100872121 0.0 80-81 5.209798589186542E-4 0.0 0.0 11.914809373469621 0.0 82-83 5.209798589186542E-4 0.0 0.0 12.878622112469131 0.0 84-85 5.209798589186542E-4 0.0 0.0 14.232388275869255 0.0 86-87 5.209798589186542E-4 0.0 0.0 16.64374355287425 0.0 88-89 5.209798589186542E-4 0.0 0.0 19.937899200816894 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position ATTGGCG 25 0.0050107003 57.01207 7 GGTATCA 985 0.0 38.60702 1 GACATAC 80 1.0675378E-5 35.65117 1 GTATCAA 1550 0.0 34.654472 1 CTAGACT 95 3.470802E-5 30.006351 4 TATCAAC 1795 0.0 29.114798 2 ATCAACG 1815 0.0 28.793974 3 TCAACGC 1840 0.0 28.660957 4 CAACGCA 1850 0.0 28.506035 5 AACGCAG 1900 0.0 27.748627 6 GTGTACA 105 6.841997E-5 27.162796 1 CCTAGAC 70 0.008365767 27.148607 3 TACATGG 815 0.0 26.232548 2 GTACATG 805 0.0 25.981806 1 ACGCAGA 2040 0.0 25.85106 7 GAGTACT 1275 0.0 25.711325 12-13 CGCAGAG 2070 0.0 25.476408 8 ACATGGG 815 0.0 25.066656 3 TAGACTG 115 1.2744736E-4 24.787855 5 GTACTTT 1365 0.0 24.177471 14-15 >>END_MODULE