FastQCFastQC Report
Thu 26 May 2016
SRR937688_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937688_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences191946
Sequences flagged as poor quality0
Sequence length101
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13930.7257249434736853No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11220.58453940170673No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7540.3928188136246653No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT6300.3282173111187522No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2960.15421003823992166No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2890.15056317922749107No Hit
GCTAAGGGTAGTTTATTTTGGACACAGACATATTAGGGCATTGTCACCAC2660.13858064247236201No Hit
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA2170.11305262938534798No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2130.11096870994967334No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTAGCT255.2645682E-575.998758
ATTCGTA301.2969083E-463.3487975
TTCGTAG301.2985822E-463.3322876
TAAGGTG407.5107528E-659.3894965
TCGTAGC352.7823055E-454.284827
GGGTAGG554.933076E-543.203621
AGCACCG459.593473E-442.2325335
TATTCGT459.593473E-442.2325334
GTATAGC500.001608467238.0191881
TACCGCA500.001612593337.9993747
CACTAGT905.6542194E-736.9534654
GGTAAGG651.3221632E-436.5473863
GTAAGGT651.3221632E-436.5473864
CTAGTAC550.002571214534.553893
ATATTCG550.002571214534.553893
TAGTCGC550.00257450434.5448887
AGTCGCT550.00257450434.5448888
GATTTCG300.0039480231.66614362-63
CTAGTCG600.003943143431.6661436
TCTCGGT300.0039480231.66614312-13