##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937688_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 191946 Sequences flagged as poor quality 0 Sequence length 101 %GC 41 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.41347566503079 33.0 31.0 34.0 31.0 34.0 2 32.613755952194886 34.0 31.0 34.0 31.0 34.0 3 32.71946797536807 34.0 31.0 34.0 31.0 34.0 4 36.28674731434883 37.0 35.0 37.0 35.0 37.0 5 36.193205380679984 37.0 35.0 37.0 35.0 37.0 6 36.28034968168131 37.0 37.0 37.0 35.0 37.0 7 36.23717608077272 37.0 37.0 37.0 35.0 37.0 8 36.23617579944359 37.0 37.0 37.0 35.0 37.0 9 37.90764590040949 39.0 38.0 39.0 35.0 39.0 10-11 37.897861898659 39.0 38.0 39.0 35.0 39.0 12-13 37.83638106550801 39.0 38.0 39.0 35.0 39.0 14-15 39.08915007345816 40.0 38.0 41.0 36.0 41.0 16-17 39.07787867421045 40.0 38.0 41.0 36.0 41.0 18-19 39.097279964156584 40.0 38.5 41.0 36.0 41.0 20-21 39.051694226501205 40.0 38.5 41.0 36.0 41.0 22-23 38.90948495931147 40.0 38.0 41.0 35.0 41.0 24-25 38.86659789732529 40.0 38.0 41.0 35.0 41.0 26-27 38.774879393162664 40.0 38.0 41.0 35.0 41.0 28-29 38.66693236639472 40.0 38.0 41.0 35.0 41.0 30-31 38.702705448407364 40.0 38.0 41.0 35.0 41.0 32-33 38.57423702499662 40.0 38.0 41.0 35.0 41.0 34-35 38.42837047919727 40.0 38.0 41.0 35.0 41.0 36-37 38.26554082919154 40.0 38.0 41.0 34.5 41.0 38-39 38.10700405322331 40.0 38.0 41.0 34.0 41.0 40-41 37.9150099507153 40.0 38.0 41.0 34.0 41.0 42-43 37.667044377064386 40.0 37.0 41.0 33.0 41.0 44-45 37.26529596865785 40.0 37.0 41.0 33.0 41.0 46-47 36.77541339751805 40.0 37.0 41.0 31.5 41.0 48-49 36.33709741281402 40.0 36.5 41.0 30.5 41.0 50-51 36.01823689996145 40.0 36.0 41.0 29.5 41.0 52-53 35.81108228355892 39.5 36.0 41.0 28.5 41.0 54-55 35.59142415054234 39.0 35.5 41.0 28.0 41.0 56-57 35.31581017577861 39.0 35.0 40.5 27.0 41.0 58-59 35.05466902149563 39.0 35.0 40.0 26.5 41.0 60-61 34.697237764788014 38.0 35.0 40.0 26.0 41.0 62-63 34.36016900586623 38.0 34.0 40.0 24.5 41.0 64-65 33.95267679451512 37.5 34.0 40.0 22.5 41.0 66-67 33.53309524553781 37.0 34.0 40.0 21.5 41.0 68-69 33.05552863826284 36.5 33.0 39.0 19.0 40.5 70-71 32.59809790253509 36.0 33.0 39.0 16.0 40.0 72-73 32.14811196899127 35.5 32.0 38.5 9.5 40.0 74-75 31.651771852500183 35.0 32.0 37.5 7.0 39.5 76-77 30.423780646640203 34.0 30.5 36.0 2.0 39.0 78-79 30.909560501391017 35.0 31.0 36.5 2.0 39.0 80-81 30.675596261448533 35.0 31.0 36.0 2.0 38.5 82-83 30.310610796786598 35.0 31.0 36.0 2.0 37.0 84-85 29.953255082158524 34.0 31.0 35.0 2.0 37.0 86-87 29.573450345409647 34.0 30.5 35.0 2.0 36.5 88-89 29.23083575588968 34.0 30.0 35.0 2.0 36.0 90-91 28.805744844904297 34.0 29.5 35.0 2.0 35.5 92-93 28.442244172840276 34.0 29.0 35.0 2.0 35.0 94-95 28.101270669875902 34.0 29.0 35.0 2.0 35.0 96-97 27.702624696529234 34.0 29.0 35.0 2.0 35.0 98-99 27.067873256019922 33.5 27.0 35.0 2.0 35.0 100-101 24.884329446823585 31.0 21.5 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.26513766135582983 1101 2 0.23761558410436123 1101 3 0.2633120773469386 1101 4 0.16888656643079258 1101 5 0.021297105582064546 1101 6 0.15362186798258648 1101 7 0.18898951083119186 1101 8 0.14578120619853507 1101 9 0.3196605831357999 1101 10-11 0.1457074047983582 1101 12-13 0.26549517926569166 1101 14-15 0.2579754962845442 1101 16-17 0.17483896116441855 1101 18-19 0.17639583385980018 1101 20-21 0.09219551206076204 1101 22-23 0.028563454196323335 1101 24-25 0.1702985146810576 1101 26-27 0.26775191707749 1101 28-29 0.46062457823244074 1101 30-31 0.2775606809232798 1101 32-33 -0.042421840816757594 1101 34-35 0.22496192065178633 1101 36-37 0.21174314878452094 1101 38-39 0.08664206670201224 1101 40-41 0.04218676944579869 1101 42-43 0.2595060365650639 1101 44-45 -0.13132041673973305 1101 46-47 -0.18733493883322438 1101 48-49 -0.32616722807033227 1101 50-51 0.07126733038295896 1101 52-53 0.3794433716486907 1101 54-55 0.6330052340787304 1101 56-57 0.21799593153841101 1101 58-59 0.6486396180349132 1101 60-61 0.9025211514413485 1101 62-63 0.71901095468872 1101 64-65 0.5201943164239324 1101 66-67 0.2907949939756378 1101 68-69 0.568442814304511 1101 70-71 0.6907385006324063 1101 72-73 0.5624985019722608 1101 74-75 0.5818640689996961 1101 76-77 0.6926028943153213 1101 78-79 0.6742412899044297 1101 80-81 0.8805600472197632 1101 82-83 0.8827888877578864 1101 84-85 0.7400206512252332 1101 86-87 0.9615047949942159 1101 88-89 1.2200309066997157 1101 90-91 0.9134456431458844 1101 92-93 1.3036429651810977 1101 94-95 1.2772163596157426 1101 96-97 1.4556258110493623 1101 98-99 1.6486855516441956 1101 100-101 2.3949890925219144 1102 1 0.11974985414229167 1102 2 0.32443333067314484 1102 3 0.041639558993921355 1102 4 0.18935986906176794 1102 5 -0.202085321119867 1102 6 -0.031777794595498676 1102 7 0.05876289097801646 1102 8 0.01724169591629021 1102 9 0.45565529381930503 1102 10-11 0.06912630954506938 1102 12-13 0.3422472653538762 1102 14-15 0.38000595545193505 1102 16-17 0.10411715185339432 1102 18-19 0.31081742886230046 1102 20-21 0.38322191304566644 1102 22-23 0.6132381156344664 1102 24-25 0.28489657222517195 1102 26-27 0.2683674840830932 1102 28-29 0.34276629543639814 1102 30-31 0.4363541695922919 1102 32-33 0.3979365559731747 1102 34-35 0.616416938662816 1102 36-37 0.6489097166564903 1102 38-39 0.9337420621422581 1102 40-41 0.9577172970095233 1102 42-43 1.209189133551071 1102 44-45 1.2712490055392252 1102 46-47 1.5154829901257827 1102 48-49 1.2040981497054233 1102 50-51 1.1961704355765264 1102 52-53 1.452422259980274 1102 54-55 1.2567797997620147 1102 56-57 1.5979033684997859 1102 58-59 1.650360925772823 1102 60-61 1.9318973768756678 1102 62-63 1.70213985897945 1102 64-65 1.689703230746126 1102 66-67 1.761395969763342 1102 68-69 1.5406397045511966 1102 70-71 1.86958351453967 1102 72-73 1.4582738884115507 1102 74-75 1.086013446593057 1102 76-77 1.214090742566654 1102 78-79 1.7265644853811537 1102 80-81 1.6230022523175691 1102 82-83 1.8992154072964382 1102 84-85 1.6032165839232384 1102 86-87 1.9703165226856107 1102 88-89 2.1023094165715897 1102 90-91 1.7131013815983032 1102 92-93 1.6085652213481687 1102 94-95 1.2319265871475444 1102 96-97 1.7824690922494888 1102 98-99 2.035455420779204 1102 100-101 2.576490164064481 1103 1 0.19956310447577863 1103 2 0.3568922012823066 1103 3 0.3357702837604819 1103 4 0.37407688614872825 1103 5 0.023983407839040183 1103 6 0.22763908909883668 1103 7 0.20949655038219106 1103 8 0.009619260548184627 1103 9 0.32858294420360323 1103 10-11 0.2619739243579957 1103 12-13 0.23481856688471225 1103 14-15 0.15566815452453397 1103 16-17 0.4315007509133011 1103 18-19 0.26898931477985144 1103 20-21 0.1910730214748071 1103 22-23 0.05892826363675141 1103 24-25 0.16733638674590878 1103 26-27 -0.19926892574984834 1103 28-29 0.10566091526742838 1103 30-31 0.5510718218821964 1103 32-33 0.7124890554967749 1103 34-35 0.6023154332318299 1103 36-37 0.25047866793642015 1103 38-39 0.5879852178305072 1103 40-41 0.6942986347469287 1103 42-43 0.796370738073712 1103 44-45 0.823827929505434 1103 46-47 1.1903543615289465 1103 48-49 0.8781596373507199 1103 50-51 0.9369282326927006 1103 52-53 0.9161521716870666 1103 54-55 1.1164317085468767 1103 56-57 1.2469696201671923 1103 58-59 1.1929036431710287 1103 60-61 1.1109134475102351 1103 62-63 1.262579419419012 1103 64-65 1.3249696983825388 1103 66-67 1.110297068664437 1103 68-69 0.8486337336434886 1103 70-71 0.9600522943148064 1103 72-73 1.4226548831393622 1103 74-75 0.7536435043965248 1103 76-77 1.2460890910389928 1103 78-79 1.5179320773267797 1103 80-81 1.4685844158188068 1103 82-83 1.1750681672532473 1103 84-85 1.4467819780666673 1103 86-87 1.6600992832776207 1103 88-89 1.8306489960672359 1103 90-91 1.913841393031955 1103 92-93 1.9442540989445583 1103 94-95 1.966840517148178 1103 96-97 1.1110595769093372 1103 98-99 1.5878120237138447 1103 100-101 2.2874410628766526 1104 1 0.2515509798458595 1104 2 0.3685233869583229 1104 3 0.2719397466111815 1104 4 0.1949213807208423 1104 5 0.043157241195871165 1104 6 -0.11252022289890817 1104 7 0.12067658703804796 1104 8 0.20847728959090261 1104 9 0.5046368001178863 1104 10-11 0.44549993591868997 1104 12-13 0.2558428161445505 1104 14-15 0.2085371803246261 1104 16-17 0.31310937913831083 1104 18-19 0.4176922613468932 1104 20-21 0.09978583692667087 1104 22-23 0.16634050105392362 1104 24-25 0.2899220345677094 1104 26-27 0.05615896089896211 1104 28-29 0.4614342128848037 1104 30-31 0.1448438506429497 1104 32-33 0.5066875610656396 1104 34-35 0.7848369333023228 1104 36-37 0.5339271382592727 1104 38-39 0.42074286621623713 1104 40-41 0.5696510949189317 1104 42-43 0.9175112991903163 1104 44-45 0.8303484426874448 1104 46-47 1.3996628346714814 1104 48-49 1.3853498248595315 1104 50-51 1.1675253002240638 1104 52-53 1.2509012200446037 1104 54-55 1.5368990707349397 1104 56-57 1.3270309482371232 1104 58-59 1.4321888539422147 1104 60-61 1.4010410380924938 1104 62-63 1.4637989316141358 1104 64-65 1.1153263880765962 1104 66-67 1.46700438573761 1104 68-69 1.3825051561727477 1104 70-71 1.581792037723801 1104 72-73 1.1910533041177906 1104 74-75 2.025037836836958 1104 76-77 1.2267214045875612 1104 78-79 1.2132302586322936 1104 80-81 1.6020963289726247 1104 82-83 1.844002327693115 1104 84-85 1.992689915469743 1104 86-87 1.66644356637363 1104 88-89 2.1023429196538714 1104 90-91 1.9567357959644234 1104 92-93 2.0484941948137276 1104 94-95 1.8190010980025448 1104 96-97 2.216568529397705 1104 98-99 2.2587199611169204 1104 100-101 3.50020364570479 1105 1 -0.11748636097002674 1105 2 -0.1854373296399956 1105 3 -0.43521544440975646 1105 4 0.05068438525353969 1105 5 -0.2978744877054922 1105 6 -0.10782564055485011 1105 7 -0.05696209200360869 1105 8 -0.30231485372875966 1105 9 -0.21579272718623344 1105 10-11 -0.07441156850608621 1105 12-13 -0.13550728098473996 1105 14-15 -0.25315071089057994 1105 16-17 -0.5045573594759745 1105 18-19 -0.14916506651841388 1105 20-21 -0.17152864612967278 1105 22-23 -0.006522654568783537 1105 24-25 -0.37077297946207466 1105 26-27 -0.5213825888528163 1105 28-29 0.024352408438439 1105 30-31 -0.16405427152411534 1105 32-33 -0.24039856023804873 1105 34-35 -0.44290427110087194 1105 36-37 -0.6235302327746126 1105 38-39 -0.8163065618001681 1105 40-41 -0.3362144349753109 1105 42-43 -0.4622102635120413 1105 44-45 -1.1853541258426574 1105 46-47 -0.8420002346140834 1105 48-49 -1.3266078699386554 1105 50-51 -1.1844334737884523 1105 52-53 -1.0293437616087502 1105 54-55 -1.2356834891614525 1105 56-57 -1.2807555831680162 1105 58-59 -0.7883485947951101 1105 60-61 -0.6424243191274428 1105 62-63 -1.0383055363331977 1105 64-65 -0.8966528910082232 1105 66-67 -0.9870480640789268 1105 68-69 -0.8575660719731673 1105 70-71 -1.121639863851854 1105 72-73 -1.1692424006091677 1105 74-75 -1.0555794737609254 1105 76-77 -1.1566425064851806 1105 78-79 -0.893342156649382 1105 80-81 -1.2145451560263005 1105 82-83 -1.38214703557043 1105 84-85 -0.8582975656440084 1105 86-87 -1.1855961487137847 1105 88-89 -1.63349239223907 1105 90-91 -1.3540239709252795 1105 92-93 -1.6253402537077513 1105 94-95 -1.9488329188331974 1105 96-97 -0.853337578955415 1105 98-99 -1.1287719447755897 1105 100-101 -0.5315522360133151 1106 1 -0.06332706893462614 1106 2 0.17161281785263327 1106 3 0.0011267384811048942 1106 4 -0.17020057049867177 1106 5 -0.16985088888543487 1106 6 0.02609766327995544 1106 7 0.06799366020878495 1106 8 -0.07623815756356578 1106 9 0.14715712532113656 1106 10-11 -0.14450006408131344 1106 12-13 0.07393224703885437 1106 14-15 -0.31438964894367416 1106 16-17 -0.04892314118689711 1106 18-19 -0.5184866004417259 1106 20-21 -0.014360504536753638 1106 22-23 0.3171941595905068 1106 24-25 -0.20422430689570348 1106 26-27 -0.18928819357258675 1106 28-29 -0.9462080635379593 1106 30-31 -0.8954407022025848 1106 32-33 -0.6595726015359844 1106 34-35 -1.4058541236082434 1106 36-37 -1.0701379023911386 1106 38-39 -0.8555985971983944 1106 40-41 -1.056568417276189 1106 42-43 -0.5999683756064371 1106 44-45 -0.4038791995889781 1106 46-47 -1.1570444824016946 1106 48-49 -0.7328615572542887 1106 50-51 -1.329507220101135 1106 52-53 -1.828163820605802 1106 54-55 -2.138751335769122 1106 56-57 -1.994147913551494 1106 58-59 -1.7740713086594155 1106 60-61 -1.99363375865547 1106 62-63 -1.5574205805809882 1106 64-65 -2.00597442493153 1106 66-67 -1.8097029313355648 1106 68-69 -2.0130232991118078 1106 70-71 -1.229427474471322 1106 72-73 -1.1392718991342399 1106 74-75 -1.6806532200736086 1106 76-77 -1.8987664002904872 1106 78-79 -1.894168115351448 1106 80-81 -1.6331882238729065 1106 82-83 -2.3188432007621707 1106 84-85 -2.205318214611559 1106 86-87 -2.3955076531385657 1106 88-89 -1.8268847226225517 1106 90-91 -2.210012171515249 1106 92-93 -1.9996765368935918 1106 94-95 -1.816974511753557 1106 96-97 -1.8159924462120536 1106 98-99 -1.30428816896438 1106 100-101 0.1948784424527581 1107 1 -0.11582706893462813 1107 2 0.17827948451930098 1107 3 0.11279340514776948 1107 4 0.024799429501328518 1107 5 -0.37818422221876347 1107 6 -0.4030690033867117 1107 7 -0.1595063397912142 1107 8 0.2929285091030991 1107 9 0.3204904586544757 1107 10-11 -0.06075006408131145 1107 12-13 -0.22815108629447423 1107 14-15 -0.1493896489436679 1107 16-17 0.26524352547977514 1107 18-19 -0.22056993377506018 1107 20-21 -0.353110504536744 1107 22-23 -0.061555840409489804 1107 24-25 -0.1379743068957069 1107 26-27 -0.505121526905917 1107 28-29 -0.28079139687130095 1107 30-31 -0.2646073688692425 1107 32-33 -0.3012392682026501 1107 34-35 0.07081254305842322 1107 36-37 0.2631954309421971 1107 38-39 -0.019348597198401762 1107 40-41 -0.1128184172761948 1107 42-43 -0.19163504227309858 1107 44-45 -0.6172125329223022 1107 46-47 0.07420551759830829 1107 48-49 0.16172177607904104 1107 50-51 0.6071594465655323 1107 52-53 0.1489195127275309 1107 54-55 0.3233319975642104 1107 56-57 0.38251875311517836 1107 58-59 0.41801202467392073 1107 60-61 0.492199574677862 1107 62-63 0.13757941941901208 1107 64-65 0.2756922417351362 1107 66-67 0.47279706866443405 1107 68-69 -0.3796899657784749 1107 70-71 -0.31942747447132547 1107 72-73 -0.2813552324675719 1107 74-75 0.5735134465930543 1107 76-77 0.6087335997095131 1107 78-79 0.3237485513152194 1107 80-81 -0.13235489053957394 1107 82-83 -0.3692598674288341 1107 84-85 -0.45365154794489015 1107 86-87 -0.11800765313856587 1107 88-89 -0.1327180559558876 1107 90-91 0.27457116181808416 1107 92-93 0.20032346310641103 1107 94-95 -0.010724511753554822 1107 96-97 -0.24349244621204846 1107 98-99 -0.28137150229771635 1107 100-101 0.4340451091194275 1108 1 0.025620299486426745 1108 2 0.1888058003087707 1108 3 0.05884603672671318 1108 4 0.15769416634343258 1108 5 -0.37950001169244985 1108 6 -0.016884792860395237 1108 7 0.09115155494562543 1108 8 -0.2222030698442694 1108 9 0.054700984970260436 1108 10-11 0.04418414644500501 1108 12-13 0.3655989137055258 1108 14-15 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2316 80-81 -0.3068371390602813 2316 82-83 -0.6639714058903756 2316 84-85 -0.547882317175663 2316 86-87 -0.20994552296104985 2316 88-89 -0.5373778192694942 2316 90-91 -0.22709303936534653 2316 92-93 -0.09494280908294073 2316 94-95 -0.9246668194458607 2316 96-97 -1.6346906710641242 2316 98-99 -1.4689824490432777 2316 100-101 -1.7920643583361944 >>END_MODULE >>Per sequence quality scores pass #Quality Count 3 1.0 4 1.0 5 6.0 6 10.0 7 13.0 8 36.0 9 75.0 10 148.0 11 235.0 12 407.0 13 649.0 14 812.0 15 1308.0 16 1920.0 17 2965.0 18 2628.0 19 1445.0 20 1164.0 21 1086.0 22 1058.0 23 1133.0 24 1329.0 25 1424.0 26 1568.0 27 1862.0 28 2094.0 29 2414.0 30 2983.0 31 3773.0 32 5050.0 33 6961.0 34 9954.0 35 15272.0 36 25966.0 37 45394.0 38 44096.0 39 4705.0 40 1.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.317871475478185 20.7901183092719 15.29212487621827 20.599885339031637 2 15.059443801902619 21.638377460327384 41.05269190293103 22.249486834838965 3 19.60082523209653 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20.877801250875898 27.655506380717444 31.585528183781335 19.881164184625323 24-25 20.888796269764775 27.790512907343253 31.822397040818984 19.498293782072988 26-27 20.468055620679056 28.07369896271289 31.867273096700583 19.590972319907472 28-29 19.86183254229164 28.50693702752408 32.04865036651888 19.582580063665397 30-31 20.371721065930345 28.131756492745318 31.727838703795356 19.76868373752898 32-33 20.22261469371594 28.489523095037146 31.393725318579186 19.89413689266773 34-35 20.436997905660967 28.263417836266452 31.704229314494704 19.59535494357788 36-37 21.103852125076845 27.93415856542986 31.420555781313492 19.541433528179798 38-39 20.480239233951217 28.349119023058567 31.686776489220925 19.483865253769288 40-41 20.35754847717587 28.714065414231086 31.30672168213977 19.621664426453272 42-43 20.519413530073777 28.910581051227545 30.75606477429036 19.81394064440832 44-45 20.70790743229867 28.803152970106176 30.32415366821919 20.16478592937597 46-47 20.696445875402457 29.426244881372888 29.897992143623725 19.97931709960093 48-49 21.03638523334688 29.844070728225645 29.211340689568942 19.90820334885853 50-51 20.86628530940994 29.711481354130846 29.1808633678222 20.241369968637013 52-53 20.910417046549792 30.25580244340827 28.755112141498866 20.078668368543074 54-55 20.604753420232775 30.006095464349347 28.870098881977224 20.519052233440654 56-57 20.875923436799933 29.864909922582395 28.992789638752566 20.266377001865106 58-59 21.06243422629281 29.41035499567587 29.01154491367364 20.515665864357686 60-61 20.655809446406803 29.466881310368542 29.631250455857376 20.24605878736728 62-63 20.58365373594657 29.11886676461088 29.84250778864889 20.45497171079366 64-65 20.39896637595991 29.229054004772177 29.928729955299925 20.44324966396799 66-67 20.19682619069947 29.10792618757359 29.506475779646358 21.188771842080588 68-69 20.121805091015183 29.03056067852417 29.60624342262928 21.24139080783137 70-71 20.307534410719683 29.40618715680452 29.335854875850497 20.9504235566253 72-73 20.061944666585045 28.44791881028729 29.764698833783548 21.72543768934411 74-75 20.06298662901709 28.666991411624654 29.385945489735366 21.884076469622887 76-77 19.81963677284236 28.1376012003376 29.89747116376481 22.145290863055234 78-79 19.87876798682963 27.696852239692415 29.377793754493453 23.046586018984506 80-81 19.934773321663386 27.737749158617525 29.671105415064652 22.656372104654434 82-83 19.921800719475062 28.109541510480838 29.65477179720285 22.31388597284125 84-85 19.280472842551664 28.662563071288467 28.88632450357002 23.17063958258985 86-87 19.186385754326736 28.021422691798737 28.609348462588436 24.18284309128609 88-89 19.264272243235077 27.603857334875432 29.13084930136601 24.00102112052348 90-91 19.272962207477224 26.668646560325538 29.716119721043405 24.342271511153836 92-93 19.322243177626575 26.563753633910242 29.259620093569932 24.854383094893247 94-95 18.153282694091047 27.88258155939691 29.310066372834026 24.654069373678013 96-97 18.02824752795057 28.356152251153972 29.97874402175612 23.63685619913934 98-99 18.098507898136955 30.158014837660986 27.71511482515734 24.02836243904472 100-101 17.88655375709593 31.101193965303885 25.819662521786025 25.192589755814165 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 93.0 1 69.5 2 47.5 3 53.5 4 56.5 5 60.0 6 92.0 7 153.0 8 229.0 9 296.0 10 331.0 11 345.0 12 406.0 13 463.5 14 467.5 15 492.0 16 527.5 17 551.5 18 587.5 19 638.5 20 682.5 21 764.0 22 888.0 23 1050.0 24 1200.0 25 1353.5 26 1598.5 27 1900.5 28 2209.0 29 2550.0 30 2943.0 31 3382.5 32 3741.0 33 4154.5 34 4646.5 35 5032.0 36 5545.5 37 5948.0 38 6144.5 39 6306.5 40 6493.5 41 6862.5 42 7095.5 43 7295.5 44 7694.0 45 7776.5 46 7592.0 47 8005.5 48 8577.5 49 7871.5 50 6756.0 51 6014.5 52 5324.5 53 4979.0 54 4609.5 55 4010.0 56 3475.5 57 3005.5 58 2491.0 59 2136.0 60 1732.5 61 1251.5 62 902.0 63 612.5 64 429.0 65 304.0 66 210.0 67 162.0 68 114.5 69 60.0 70 37.0 71 23.5 72 18.5 73 19.0 74 19.0 75 12.5 76 6.5 77 3.0 78 1.0 79 2.0 80 2.0 81 1.5 82 2.5 83 1.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.03959446927781772 2 0.0 3 0.0 4 0.0 5 0.010940577037291737 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.003907348941889907 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 2.6048992945932714E-4 24-25 5.209798589186543E-4 26-27 0.0015629395767559626 28-29 0.002604899294593271 30-31 5.209798589186543E-4 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.009377637460535776 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 5.209798589186543E-4 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 2.6048992945932714E-4 74-75 2.6048992945932714E-4 76-77 0.0 78-79 0.0 80-81 0.0 82-83 2.6048992945932714E-4 84-85 2.6048992945932714E-4 86-87 0.0 88-89 0.0 90-91 0.008596167672157795 92-93 0.002083919435674617 94-95 0.0 96-97 0.0 98-99 0.0010419597178373085 100-101 0.011722046825669718 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 191946.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 66.74004984831996 #Duplication Level Percentage of deduplicated Percentage of total 1 83.95253241063699 56.029961979786094 2 9.782371101980956 13.057518699619472 3 2.6884778621747483 5.38287439612942 4 1.1254093071980227 3.00439493048637 5 0.621163098475922 2.072822807810995 6 0.41183416239721077 1.6491499516578565 7 0.244345932921241 1.1415361820378505 8 0.20373823903531513 1.0878000183380687 9 0.15229760796166184 0.914791495242708 >10 0.7525622118764487 9.042658752176477 >50 0.04351204356100582 2.1001170756435004 >100 0.017800381456775108 1.943037294372868 >500 0.0019778201618639007 0.913438728061158 >1k 0.0019778201618639007 1.6598976886371477 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1393 0.7257249434736853 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1122 0.58453940170673 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 754 0.3928188136246653 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 630 0.3282173111187522 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 296 0.15421003823992166 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 289 0.15056317922749107 No Hit GCTAAGGGTAGTTTATTTTGGACACAGACATATTAGGGCATTGTCACCAC 266 0.13858064247236201 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 217 0.11305262938534798 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 213 0.11096870994967334 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0010419597178373083 0.0 18-19 0.0 0.0 0.0 0.0015629395767559626 0.0 20-21 0.0 0.0 0.0 0.0015629395767559626 0.0 22-23 0.0 0.0 0.0 0.0015629395767559626 0.0 24-25 0.0 0.0 0.0 0.0015629395767559626 0.0 26-27 0.0 0.0 0.0 0.002083919435674617 0.0 28-29 0.0 0.0 0.0 0.006772738165942505 0.0 30-31 0.0 0.0 0.0 0.016671355485396933 0.0 32-33 0.0 0.0 0.0 0.030477321746741268 0.0 34-35 0.0 0.0 0.0 0.045064757796463586 0.0 36-37 0.0 0.0 0.0 0.06095464349348254 0.0 38-39 0.0 0.0 0.0 0.08283579756806601 0.0 40-41 0.0 0.0 0.0 0.10419597178373083 0.0 42-43 0.0 0.0 0.0 0.16020130661748616 0.0 44-45 0.0 0.0 0.0 0.22428182926448065 0.0 46-47 0.0 0.0 0.0 0.293051170641743 0.0 48-49 0.0 0.0 0.0 0.3847436258114261 0.0 50-51 0.0 0.0 0.0 0.4894605774540757 0.0 52-53 0.0 0.0 0.0 0.7022808498223458 0.0 54-55 0.0 0.0 0.0 0.8718597939003678 0.0 56-57 0.0 0.0 0.0 1.2456628426745022 0.0 58-59 0.0 0.0 0.0 1.7359048899169558 0.0 60-61 0.0 0.0 0.0 2.217029789628333 0.0 62-63 0.0 0.0 0.0 3.006835255749013 0.0 64-65 0.0 0.0 0.0 3.423879632813396 0.0 66-67 0.0 0.0 0.0 4.1691413209965305 0.0 68-69 0.0 0.0 0.0 5.004532524772593 0.0 70-71 0.0 0.0 0.0 5.770372917383014 0.0 72-73 0.0 0.0 0.0 7.15800277161285 0.0 74-75 0.0 0.0 0.0 8.429454117303825 0.0 76-77 0.0 0.0 0.0 9.345597199212278 0.0 78-79 0.0 0.0 0.0 10.28570535463099 0.0 80-81 0.0 0.0 0.0 12.024996613630917 0.0 82-83 0.0 0.0 0.0 12.999489439738259 0.0 84-85 0.0 0.0 0.0 14.364717160034592 0.0 86-87 0.0 0.0 0.0 16.80212142998552 0.0 88-89 0.0 0.0 0.0 20.1306617486168 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGTAGCT 25 5.2645682E-5 75.99875 8 ATTCGTA 30 1.2969083E-4 63.348797 5 TTCGTAG 30 1.2985822E-4 63.332287 6 TAAGGTG 40 7.5107528E-6 59.389496 5 TCGTAGC 35 2.7823055E-4 54.28482 7 GGGTAGG 55 4.933076E-5 43.20362 1 AGCACCG 45 9.593473E-4 42.232533 5 TATTCGT 45 9.593473E-4 42.232533 4 GTATAGC 50 0.0016084672 38.019188 1 TACCGCA 50 0.0016125933 37.999374 7 CACTAGT 90 5.6542194E-7 36.953465 4 GGTAAGG 65 1.3221632E-4 36.547386 3 GTAAGGT 65 1.3221632E-4 36.547386 4 CTAGTAC 55 0.0025712145 34.55389 3 ATATTCG 55 0.0025712145 34.55389 3 TAGTCGC 55 0.002574504 34.544888 7 AGTCGCT 55 0.002574504 34.544888 8 GATTTCG 30 0.00394802 31.666143 62-63 CTAGTCG 60 0.0039431434 31.666143 6 TCTCGGT 30 0.00394802 31.666143 12-13 >>END_MODULE