FastQCFastQC Report
Thu 26 May 2016
SRR937687_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937687_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences842154
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT41870.49717747585358496No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT33540.3982644504449305No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT32160.381877898816606No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT26440.3139568297484783No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT20220.24009860429327654No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC19520.23178658535137278No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16840.19996342711665563No Hit
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT13800.1638655162832451No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT12130.14403541395041763No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN10730.12741137606661013No Hit
ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC9910.11767443959180862No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC9840.11684323769761826No Hit
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA8470.10057542919703522No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATAGCG700.00834836927.1707525
TACCTGG7000.027.1675152
GTACGCC700.00838725727.144889
GTACCTG7600.024.3985981
GTACATG29900.024.329551
TACATGG29200.023.9343952
ACATGGG29400.023.6098653
GTAAGCG853.1532672E-622.35061894-95
CATGGGG16150.022.0814934
GTATAGG2606.166374E-1021.9443021
GAGTACT29250.021.76101112-13
GCCGCGC1100.00287548521.5925188
GTACTTT31000.020.45595414-15
AGAGTAC44250.019.96640210-11
GTGTAGC5250.019.9240321
AGTACTT30400.019.84404812-13
GTGTAGG2952.9758667E-919.3407381
TATAGGG2302.0387306E-618.6038442
GTGTATA3651.1459633E-1018.2368141
TATTAGG2905.1541065E-818.0336112
Produced by FastQC (version 0.11.5)