##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937687_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 842154 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.7163962885648 33.0 31.0 34.0 30.0 34.0 2 31.860045787350057 33.0 31.0 34.0 30.0 34.0 3 31.88187077422894 34.0 31.0 34.0 30.0 34.0 4 35.45406303360193 37.0 35.0 37.0 33.0 37.0 5 35.440785177057876 37.0 35.0 37.0 33.0 37.0 6 35.511431400907675 37.0 35.0 37.0 33.0 37.0 7 35.45028343984592 37.0 35.0 37.0 33.0 37.0 8 35.4375506142582 37.0 35.0 37.0 33.0 37.0 9 37.06308941120032 39.0 37.0 39.0 33.0 39.0 10-11 36.93821973178302 39.0 37.0 39.0 33.0 39.0 12-13 36.81497089605939 39.0 37.0 39.0 33.0 39.0 14-15 37.964184104094976 40.0 38.0 41.0 33.0 41.0 16-17 37.870508838050995 40.0 38.0 41.0 33.0 41.0 18-19 37.78857133018427 40.0 38.0 41.0 33.0 41.0 20-21 37.67355139321312 40.0 37.5 41.0 32.0 41.0 22-23 37.591949334682255 40.0 37.0 41.0 32.0 41.0 24-25 37.61284100057709 40.0 37.0 41.0 32.0 41.0 26-27 37.50668464437621 40.0 37.0 41.0 32.0 41.0 28-29 37.377646487459536 40.0 37.0 41.0 32.0 41.0 30-31 37.15455308649012 39.5 37.0 41.0 31.0 41.0 32-33 37.016067132614694 39.0 36.0 41.0 31.0 41.0 34-35 37.01765472823261 40.0 36.0 41.0 31.0 41.0 36-37 36.874240340840274 39.5 36.0 41.0 30.5 41.0 38-39 36.71321397274133 39.0 36.0 41.0 30.0 41.0 40-41 36.50332718243932 39.0 36.0 41.0 30.0 41.0 42-43 36.22808536205967 39.0 35.0 41.0 30.0 41.0 44-45 35.98716505532242 39.0 35.0 40.5 29.0 41.0 46-47 35.640531304250764 39.0 35.0 40.0 27.5 41.0 48-49 35.305840736967355 39.0 35.0 40.0 26.5 41.0 50-51 34.43492223512564 38.0 34.0 39.5 25.0 40.5 52-53 34.5493656742116 38.0 34.0 39.5 25.5 40.5 54-55 34.81768298909701 38.0 34.0 40.0 25.5 41.0 56-57 34.53340541041187 38.0 34.0 40.0 23.5 41.0 58-59 34.25725164281117 38.0 34.0 40.0 23.0 41.0 60-61 33.8744309235603 38.0 33.5 40.0 22.0 41.0 62-63 33.491641077522644 37.0 33.0 40.0 21.5 41.0 64-65 33.088572280129284 36.5 32.5 39.5 19.5 41.0 66-67 32.587324289856724 36.0 32.0 39.0 18.0 40.5 68-69 32.040069868456364 35.5 31.0 39.0 13.5 40.0 70-71 31.538489397426126 35.0 31.0 38.0 9.5 40.0 72-73 31.055222085271815 35.0 30.5 37.5 7.5 39.5 74-75 30.50086801226379 34.5 30.0 37.0 4.5 39.0 76-77 30.002971546771732 34.0 29.0 36.0 2.0 39.0 78-79 29.396175758827958 34.0 29.0 36.0 2.0 38.5 80-81 28.76393272489355 34.0 28.0 35.0 2.0 37.0 82-83 28.219490140758104 34.0 26.5 35.0 2.0 37.0 84-85 27.66874645254906 33.0 26.0 35.0 2.0 36.0 86-87 27.135695490373493 33.0 25.5 35.0 2.0 36.0 88-89 26.610566475965204 32.5 25.0 35.0 2.0 35.5 90-91 25.993613994590063 32.0 23.5 34.5 2.0 35.0 92-93 25.307131474765896 32.0 20.0 34.0 2.0 35.0 94-95 24.50828292687561 31.0 15.5 34.0 2.0 35.0 96-97 23.63095704586097 31.0 4.5 34.0 2.0 35.0 98-99 22.535319549631062 31.0 2.0 34.0 2.0 35.0 100-101 19.793115035967293 27.0 2.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 -0.019562626882922984 1101 2 0.3148058230020432 1101 3 0.14316478057035553 1101 4 -0.023092925399460285 1101 5 -0.057073051166980804 1101 6 0.009742256481217737 1101 7 0.04950813848120106 1101 8 0.08272819804162168 1101 9 0.20802988674110878 1101 10-11 0.17801145934657825 1101 12-13 0.15641794867875802 1101 14-15 0.257185357170556 1101 16-17 0.19373964229020402 1101 18-19 0.298656053912147 1101 20-21 0.2088998293236628 1101 22-23 0.2554560783973798 1101 24-25 0.34586228810349695 1101 26-27 0.10940271977191429 1101 28-29 0.23556962468249765 1101 30-31 0.16121860165532098 1101 32-33 0.2084787386142537 1101 34-35 0.07441803527515134 1101 36-37 0.1700629232113684 1101 38-39 0.1926661285347464 1101 40-41 0.05939430749307917 1101 42-43 0.07244964461612113 1101 44-45 0.09259567055062945 1101 46-47 0.09274184691788179 1101 48-49 0.22543652494204736 1101 50-51 0.13371282242464844 1101 52-53 -0.14167210294147736 1101 54-55 -0.09064386620816833 1101 56-57 -0.23492955540308458 1101 58-59 -0.20369205046030459 1101 60-61 0.11914175925108594 1101 62-63 0.12968328747297164 1101 64-65 0.14827098540583705 1101 66-67 0.27699510757581436 1101 68-69 0.01360692725522128 1101 70-71 0.005264944693433193 1101 72-73 -0.006325871984817155 1101 74-75 0.13636320628069143 1101 76-77 0.13638236773077494 1101 78-79 0.09691893227647341 1101 80-81 0.20035294885716937 1101 82-83 0.3397375450767903 1101 84-85 0.2614957951265069 1101 86-87 0.46988150755388247 1101 88-89 0.5431324173836671 1101 90-91 0.7699825457612626 1101 92-93 0.5399184622955424 1101 94-95 0.4567210716527228 1101 96-97 0.7503278504190511 1101 98-99 0.9684213020952654 1101 100-101 1.0278336102078036 1102 1 0.2445896107521648 1102 2 0.35372604644673444 1102 3 0.2885023725693152 1102 4 0.2425287837475878 1102 5 0.12186336448187518 1102 6 0.3047939729901188 1102 7 0.3503285692556588 1102 8 0.22755181315408635 1102 9 0.3334151278754902 1102 10-11 0.3097755207687527 1102 12-13 0.3755527671347849 1102 14-15 0.5187923464833233 1102 16-17 0.6109705241856602 1102 18-19 0.3996921231096664 1102 20-21 0.7001037718741827 1102 22-23 0.6872947244354108 1102 24-25 0.7313029266714679 1102 26-27 0.42954800519333247 1102 28-29 0.5996266026916288 1102 30-31 0.6154973626232163 1102 32-33 0.5867913293089586 1102 34-35 0.7149549672195903 1102 36-37 0.8165434374974083 1102 38-39 0.9517418782937028 1102 40-41 0.8734679994122416 1102 42-43 0.818208369180546 1102 44-45 0.8081225065205118 1102 46-47 0.8293398029902548 1102 48-49 0.9429999460185741 1102 50-51 0.8997784646155793 1102 52-53 1.009126493476522 1102 54-55 0.87174500561256 1102 56-57 0.9708094989180864 1102 58-59 0.843673045098825 1102 60-61 0.9952414475801419 1102 62-63 0.9478629977523241 1102 64-65 0.6701053418623317 1102 66-67 0.8037933986992769 1102 68-69 0.8496966065606948 1102 70-71 1.1229637194575552 1102 72-73 1.0133598292168458 1102 74-75 0.8873583119068087 1102 76-77 0.842921854101693 1102 78-79 1.1599632212396926 1102 80-81 1.1800402344988576 1102 82-83 1.3903265896401322 1102 84-85 1.3126350461501133 1102 86-87 1.4926510700745332 1102 88-89 1.3574703436608324 1102 90-91 1.5660564193113586 1102 92-93 1.462022946454038 1102 94-95 1.9838333524154983 1102 96-97 1.7723916608427395 1102 98-99 1.9558689624376981 1102 100-101 1.9033285061854492 1103 1 0.10928002226694034 1103 2 0.07873281850935854 1103 3 0.18211030090092706 1103 4 0.12418442625175174 1103 5 0.17389978576784415 1103 6 0.049775901565631386 1103 7 0.13274300653772997 1103 8 0.19815620490415142 1103 9 0.17092015970688124 1103 10-11 0.09771552946720874 1103 12-13 0.09517588431251056 1103 14-15 0.2509386532539821 1103 16-17 0.15258282548902713 1103 18-19 0.2071367513800837 1103 20-21 -0.0806832119516443 1103 22-23 0.07626095693461821 1103 24-25 -0.019722595640722318 1103 26-27 -0.015639837254497024 1103 28-29 0.0803950240509792 1103 30-31 0.07978781773820032 1103 32-33 -0.04959207627769757 1103 34-35 0.17384126239949893 1103 36-37 0.16073041765391594 1103 38-39 0.30786549568672683 1103 40-41 0.11648414120358552 1103 42-43 0.19692477462366043 1103 44-45 0.07701979888259558 1103 46-47 0.15189611363528144 1103 48-49 0.1383715245081234 1103 50-51 -0.026887381849078906 1103 52-53 -0.009416547163404232 1103 54-55 0.027113929237728485 1103 56-57 0.0848105630222733 1103 58-59 -0.036070771279533176 1103 60-61 0.1488949618759392 1103 62-63 0.3034394540695473 1103 64-65 0.1292555046616357 1103 66-67 0.3108471359605147 1103 68-69 0.1704571064954017 1103 70-71 0.08757524048980159 1103 72-73 0.00612748217642789 1103 74-75 -0.003949813165363025 1103 76-77 0.09165242961369557 1103 78-79 0.19827474907098264 1103 80-81 0.20336364986318145 1103 82-83 0.23867879301569417 1103 84-85 0.37584402175018283 1103 86-87 0.5228825289225121 1103 88-89 0.27242133119483825 1103 90-91 0.4764358598688361 1103 92-93 0.34694320878917395 1103 94-95 0.6427269862449414 1103 96-97 0.9285149544718188 1103 98-99 1.0040774146193847 1103 100-101 1.2600214085242136 1104 1 0.3945582223277455 1104 2 0.45466065878502704 1104 3 0.34309945667269304 1104 4 0.26505804538624034 1104 5 0.3216204708798358 1104 6 0.23466695254364112 1104 7 0.323552776477932 1104 8 0.31257396106103386 1104 9 0.17578891414085263 1104 10-11 0.3450936585844673 1104 12-13 0.3346650907582358 1104 14-15 0.47306087885446857 1104 16-17 0.5247303880713758 1104 18-19 0.5417045024695781 1104 20-21 0.7127230918894369 1104 22-23 0.6893247047809723 1104 24-25 0.7802571347737839 1104 26-27 0.7368442004469316 1104 28-29 0.5803382692116159 1104 30-31 0.6152251340450832 1104 32-33 0.5217122715483882 1104 34-35 0.5234145876249983 1104 36-37 0.5161961113804807 1104 38-39 0.6303525738259808 1104 40-41 0.6634589055696551 1104 42-43 0.8284703305170424 1104 44-45 0.8728442642722811 1104 46-47 0.7960534056186646 1104 48-49 0.7577918143631948 1104 50-51 0.7631866021379494 1104 52-53 0.8593966686063297 1104 54-55 0.8433681098397372 1104 56-57 0.8204161168886941 1104 58-59 0.8529147359668485 1104 60-61 1.037345352065465 1104 62-63 0.8172572304960255 1104 64-65 0.7483798585685975 1104 66-67 0.9410488183361139 1104 68-69 0.8731387726553343 1104 70-71 0.7141344783533832 1104 72-73 0.7357717509511303 1104 74-75 0.7484572001112113 1104 76-77 0.8940016745716406 1104 78-79 0.956505214257966 1104 80-81 1.072728198358167 1104 82-83 0.9350160789181992 1104 84-85 0.8977088236149839 1104 86-87 0.9573610938358605 1104 88-89 0.9910166489205956 1104 90-91 0.8154588658483632 1104 92-93 0.7004265370551117 1104 94-95 0.8507294185952006 1104 96-97 0.9698324775284703 1104 98-99 0.8338595165754015 1104 100-101 0.8403903149800804 1105 1 0.3971385967086789 1105 2 0.4980653427437254 1105 3 0.45455584884930644 1105 4 0.3840457059251463 1105 5 0.3138692747075851 1105 6 0.23055547768424134 1105 7 0.3995974335752237 1105 8 0.16735948947537338 1105 9 0.35701446605892784 1105 10-11 0.39075218288564884 1105 12-13 0.49461136793443217 1105 14-15 0.5488167757738012 1105 16-17 0.6716522381511183 1105 18-19 0.5358478752556124 1105 20-21 0.43556284677905666 1105 22-23 0.6606249650009062 1105 24-25 0.4476918699370529 1105 26-27 0.5231583110823763 1105 28-29 0.41491730052195663 1105 30-31 0.3466538253906677 1105 32-33 0.5360797692399828 1105 34-35 0.4739845532088154 1105 36-37 0.5061801624171594 1105 38-39 0.5627818527649566 1105 40-41 0.6862071637749736 1105 42-43 0.689362633043693 1105 44-45 0.8041504004260602 1105 46-47 0.7630978108902156 1105 48-49 0.8227225622856409 1105 50-51 0.7193697633903611 1105 52-53 0.8075734502734164 1105 54-55 0.837116955638443 1105 56-57 0.8626951398048348 1105 58-59 0.8941151053112577 1105 60-61 1.1431532929703536 1105 62-63 1.241813597490065 1105 64-65 1.029408986412129 1105 66-67 1.259876989241846 1105 68-69 1.0521835136945263 1105 70-71 1.0824388517270087 1105 72-73 1.1944429504810508 1105 74-75 1.1253354003966152 1105 76-77 1.100841261577429 1105 78-79 1.0806813243897544 1105 80-81 1.2567263516361002 1105 82-83 1.3087137201504682 1105 84-85 1.0528452292223065 1105 86-87 1.1856411240549498 1105 88-89 1.0232234817922468 1105 90-91 1.0927013740326998 1105 92-93 0.7274575116207167 1105 94-95 1.2091580259988781 1105 96-97 1.1396033161081753 1105 98-99 1.2986928918796892 1105 100-101 1.166420701952294 1106 1 0.0885733738428982 1106 2 0.20935762848199246 1106 3 0.0981752142484531 1106 4 0.23854289387109162 1106 5 0.31207501633437573 1106 6 0.195121497998187 1106 7 0.2980224734476309 1106 8 0.16552850651557804 1106 9 0.39669800504994157 1106 10-11 0.1501694161602245 1106 12-13 0.0385287271218786 1106 14-15 0.1802199697635558 1106 16-17 0.2826470547380424 1106 18-19 0.31041662368169654 1106 20-21 0.22340491007125962 1106 22-23 0.1696277350840063 1106 24-25 -0.033379228862578714 1106 26-27 0.18222298832571937 1106 28-29 0.014126147999498073 1106 30-31 0.26401301283296164 1106 32-33 0.18072742306353717 1106 34-35 0.41705095126136627 1106 36-37 -0.01395540377102833 1106 38-39 -0.08805651708311046 1106 40-41 -0.026882003521251363 1106 42-43 -0.048688760392046504 1106 44-45 -0.00992088724287754 1106 46-47 -0.006219321654064913 1106 48-49 0.02601151133290358 1106 50-51 0.1307244809258279 1106 52-53 -0.05295181624214962 1106 54-55 -0.09947279925117414 1106 56-57 -0.013826307353731693 1106 58-59 0.1179147359668491 1106 60-61 -0.0773516176315141 1106 62-63 -0.18759125435246204 1106 64-65 0.021864707053445898 1106 66-67 0.07400336379065209 1106 68-69 -0.007050621284065528 1106 70-71 -0.054501885282977725 1106 72-73 0.26115053882992 1106 74-75 0.1490253819293983 1106 76-77 0.1250244018443638 1106 78-79 0.335974911227666 1106 80-81 0.31859941047937923 1106 82-83 0.4589933516454714 1106 84-85 0.760095187251352 1106 86-87 0.9412626089873761 1106 88-89 1.2714711943751382 1106 90-91 0.974057350696846 1106 92-93 0.8966765370551109 1106 94-95 1.3436460852618701 1106 96-97 1.7991506593466546 1106 98-99 1.88473072869661 1106 100-101 1.9606554664952291 1107 1 0.17051117288596274 1107 2 0.2999178358185155 1107 3 0.33068119510969396 1107 4 0.1940353181135137 1107 5 0.3155140115496877 1107 6 0.23843290150695395 1107 7 0.2992684862067989 1107 8 0.3613518717468409 1107 9 0.4016820560866279 1107 10-11 0.503762916957676 1107 12-13 0.508375218349947 1107 14-15 0.5144504322834962 1107 16-17 0.5326719749932209 1107 18-19 0.6439243988013175 1107 20-21 0.5316933869452569 1107 22-23 0.43443036666295143 1107 24-25 0.407136122014613 1107 26-27 0.4674103886447014 1107 28-29 0.4246125913806793 1107 30-31 0.5628068724820849 1107 32-33 0.553609201372943 1107 34-35 0.6207989576409503 1107 36-37 0.6887459518908443 1107 38-39 0.6913743042885017 1107 40-41 0.5080561942458814 1107 42-43 0.3744820928774928 1107 44-45 0.37258010956733045 1107 46-47 0.6197027277877254 1107 48-49 0.5938444459421461 1107 50-51 0.43123783818260364 1107 52-53 0.21187573559198114 1107 54-55 0.45545144317306807 1107 56-57 0.546256427893475 1107 58-59 0.7127612271949175 1107 60-61 0.5121370187321261 1107 62-63 0.46766193544020496 1107 64-65 0.35584596702155125 1107 66-67 0.608517917219686 1107 68-69 0.9525038045532561 1107 70-71 0.926708242308731 1107 72-73 1.1596004989575057 1107 74-75 0.7852644170171139 1107 76-77 0.7663970094998689 1107 78-79 1.025842335470088 1107 80-81 1.1437589001125552 1107 82-83 1.0556839417571133 1107 84-85 0.9154231378095616 1107 86-87 1.4666314287640922 1107 88-89 1.3022227892714717 1107 90-91 1.2709821912072137 1107 92-93 1.651236943753677 1107 94-95 1.903280255915778 1107 96-97 2.4444855875763203 1107 98-99 2.1234418530985266 1107 100-101 2.0157541507057566 1108 1 0.0703833803831202 1108 2 0.12234542006690674 1108 3 0.2411199493694447 1108 4 0.13670618142286628 1108 5 -0.05900085024508428 1108 6 0.1180831286936268 1108 7 0.012528141640345325 1108 8 -0.016357802618927053 1108 9 0.010116361274057795 1108 10-11 0.2216077200625648 1108 12-13 0.406484362613476 1108 14-15 0.46178335541866744 1108 16-17 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-1.6420658253707998 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 3884.0 3 907.0 4 774.0 5 877.0 6 987.0 7 1117.0 8 1385.0 9 1652.0 10 2032.0 11 2584.0 12 3256.0 13 4167.0 14 4471.0 15 5730.0 16 6883.0 17 8598.0 18 7752.0 19 6114.0 20 6149.0 21 6752.0 22 7163.0 23 8074.0 24 9239.0 25 10648.0 26 12068.0 27 14065.0 28 16443.0 29 19387.0 30 23619.0 31 28967.0 32 36618.0 33 47354.0 34 63790.0 35 86790.0 36 120357.0 37 151335.0 38 100433.0 39 9733.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.54799316467303 18.35404373894767 14.495656471232682 22.60230662514662 2 15.15093075054076 19.715102360536225 41.07266365959899 24.06130322932403 3 20.165135190151886 23.659139635183603 29.880636494258006 26.295088680406504 4 12.5801482370364 15.918933912376817 37.591160063142404 33.90975778744438 5 13.511196726405558 37.141057679600635 34.71455461328463 14.633190980709177 6 32.008074790546736 35.53918973735519 17.473332849575502 14.979402622522574 7 28.46249653533247 32.346255006667626 20.871989902798976 18.319258555200925 8 25.90757956971979 34.593482152947544 19.757677414749917 19.741260862582752 9 25.44868614988205 17.476603606143552 21.15210064940086 35.92260959457354 10-11 25.12226691363929 26.11039533464433 27.362686909856183 21.404650841860192 12-13 26.092477559245257 23.657599166367298 27.922413157312487 22.327510117074958 14-15 22.673214856070885 25.089886925370507 26.859908438754065 25.376989779804543 16-17 21.822174663288898 28.536708246366118 27.360041018588632 22.28107607175636 18-19 21.679650559027046 27.53917471691784 29.299583540016915 21.481591184038198 20-21 22.448404265758846 25.964663739325044 29.479376872335912 22.107555122580198 22-23 22.05254471653508 25.885959825743043 29.825415941835047 22.23607951588682 24-25 22.454779086178604 26.029272783828777 29.566415698748095 21.949532431244524 26-27 21.997652004182143 25.92317034150612 30.21159319961414 21.867584454697596 28-29 21.533446939619154 26.99142599680594 29.724817025478146 21.750310038096767 30-31 21.801618530117686 26.11882901933492 29.585850204175106 22.493702246372287 32-33 21.702307376585985 26.183935805083486 29.907681143040694 22.206075675289835 34-35 22.54225371381801 26.115156612660112 29.50193735969459 21.840652313827288 36-37 22.098383274308127 25.896930391885743 30.015313844607594 21.989372489198537 38-39 21.87240576304165 26.580566741911927 29.742057026234352 21.80497046881207 40-41 21.925962136593245 26.67286344838414 29.56929393559867 21.831880479423948 42-43 21.566163232557294 26.519000855875657 29.198385315338733 22.71645059622832 44-45 21.98235996309974 26.303389839581413 29.13930918479809 22.57494101252075 46-47 22.466836417577724 26.71417788872453 28.89810008642189 21.920885607275856 48-49 22.225235802764328 26.735911610096608 28.81534166271134 22.22351092442773 50-51 21.612948699723237 26.995709660668837 29.314979190694373 22.076362448913557 52-53 22.191398439270834 27.290748125536552 28.241915995242973 22.275937439949644 54-55 21.820797560169094 26.793416072528824 28.617460872544054 22.768325494758027 56-57 22.176882237086232 27.028941208932363 28.427893169427502 22.366283384553906 58-59 22.712289943013523 26.795181095451014 28.391254422056733 22.10127453947873 60-61 22.186332079622993 26.826170336123212 29.275989712637696 21.7115078716161 62-63 21.872089800937925 27.136881491639443 29.083079901949706 21.90794880547293 64-65 21.79639657425985 27.525448644225637 28.586626483240586 22.09152829827393 66-67 21.8631609430869 27.152132791887105 28.139366308114138 22.845339956911854 68-69 21.67252554723689 26.962110457789596 28.758728106510105 22.60663588846341 70-71 22.35388915318744 26.85662464319696 28.345563748810655 22.443922454804948 72-73 22.086653504911872 26.610035441592732 28.968150139149877 22.33516091434552 74-75 21.550545067042176 27.757744281137086 28.26082819743724 22.43088245438349 76-77 21.896923401712094 27.069129055374013 28.131413296542206 22.902534246371683 78-79 22.112897182922694 27.028896302771884 28.333660416406488 22.524546097898938 80-81 22.293859492632105 27.088224450232513 28.099749051509853 22.51816700562553 82-83 22.114169531519927 26.98605543453892 28.63842310864677 22.261351925294377 84-85 22.028524452716425 27.227205560989827 28.205670996571953 22.5385989897218 86-87 21.87164414465305 27.3287777937663 28.352703890324555 22.4468741712561 88-89 21.849339627746396 27.119295143078176 27.867941009797807 23.163424219377617 90-91 22.10012464141806 26.79951047320923 28.090108133566122 23.010256751806587 92-93 22.391915910051775 26.836583670437996 28.36234029028562 22.409160129224613 94-95 22.15126004446831 26.564195696243026 28.472562910051874 22.81198134923679 96-97 21.462971981834606 26.746266633128357 29.000825970068846 22.789935414968195 98-99 21.78411997285994 27.093676615841495 28.056839515070642 23.065363896227925 100-101 21.942772615471146 26.197875451037095 28.727488277048703 23.13186365644306 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 975.0 1 561.0 2 157.5 3 181.5 4 176.0 5 148.0 6 231.0 7 489.0 8 817.5 9 1034.0 10 971.5 11 871.0 12 916.0 13 981.0 14 1076.5 15 1152.0 16 1197.5 17 1242.5 18 1294.5 19 1387.5 20 1531.0 21 1801.0 22 2217.0 23 2634.0 24 3176.0 25 3970.5 26 4904.0 27 6090.5 28 7219.0 29 8405.0 30 9709.5 31 11017.0 32 12546.0 33 14212.5 34 15964.0 35 17341.0 36 19128.0 37 21114.0 38 22538.5 39 23767.0 40 24799.5 41 25915.5 42 26978.0 43 28097.0 44 29333.5 45 30570.5 46 31611.5 47 34969.0 48 41152.5 49 40778.5 50 34964.5 51 31275.0 52 29083.5 53 28015.5 54 27032.0 55 24951.0 56 22491.5 57 20306.0 58 18005.5 59 15548.0 60 12778.5 61 10130.5 62 7830.5 63 5752.5 64 4104.5 65 3011.5 66 2226.0 67 1636.5 68 1174.5 69 829.0 70 632.5 71 445.0 72 304.0 73 217.5 74 159.0 75 116.0 76 70.0 77 54.0 78 46.5 79 26.0 80 14.5 81 12.0 82 7.5 83 4.0 84 4.5 85 5.0 86 3.5 87 4.5 88 4.0 89 2.0 90 2.5 91 2.0 92 2.0 93 2.0 94 1.0 95 0.0 96 0.5 97 1.0 98 0.5 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.2848647634518152 2 0.25327909147258104 3 0.25114171517323436 4 0.2550602383887033 5 0.2637284867138314 6 0.17965835227286223 7 0.18108326980575998 8 0.18274567359414073 9 0.18203321482769186 10-11 0.1870204261928341 12-13 0.17763971910125703 14-15 0.17983646696447445 16-17 0.18517990771284112 18-19 0.1777584622289985 20-21 0.1733055949386929 22-23 0.17116821863934625 24-25 0.17520548498255664 26-27 0.17063387456450957 28-29 0.18185510013607964 30-31 0.18719854088444632 32-33 0.18868282998121483 34-35 0.18725791244831705 36-37 0.1676059248071018 38-39 0.1600657361955177 40-41 0.16564666319936736 42-43 0.17746160440964479 44-45 0.17924275132576703 46-47 0.1793614944535085 48-49 0.17989583852834518 50-51 0.1833987607967189 52-53 0.20346634938502936 54-55 0.17099010394773403 56-57 0.15982824994003472 58-59 0.15917516273745658 60-61 0.156325327671661 62-63 0.16160939685615697 64-65 0.15947202055681026 66-67 0.15668155705488546 68-69 0.16713095229613587 70-71 0.16077819496196658 72-73 0.15840333240713694 74-75 0.15911579117358582 76-77 0.1628561996974425 78-79 0.16493420443291845 80-81 0.15959076368455177 82-83 0.1615500252922862 84-85 0.15395046511683136 86-87 0.15116000161490656 88-89 0.15466292388328026 90-91 0.1593532774290688 92-93 0.15347549260586543 94-95 0.15798773146004175 96-97 0.15727527269359284 98-99 0.15798773146004175 100-101 0.13958254666011205 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 842154.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 69.93781820188464 #Duplication Level Percentage of deduplicated Percentage of total 1 86.38609235751407 60.416548224710354 2 8.519245911841589 11.916349435990519 3 2.163564211628512 4.539448815029362 4 0.9085947951833802 2.541805504188554 5 0.4862939726238775 1.7005169725020506 6 0.3132348428123305 1.314417689466281 7 0.23118778377782703 1.1318138434645197 8 0.162599873533851 0.9097504315887924 9 0.11619059380422532 0.7313504963624041 >10 0.6623906377970266 8.082841893963892 >50 0.03424920041154455 1.602086287464409 >100 0.012948356838696011 1.5119608298478966 >500 0.0017037311165315806 0.8933068124853185 >1k 0.0017037311165315806 2.7078027629356893 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 4187 0.49717747585358496 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 3354 0.3982644504449305 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3216 0.381877898816606 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2644 0.3139568297484783 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 2022 0.24009860429327654 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 1952 0.23178658535137278 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1684 0.19996342711665563 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 1380 0.1638655162832451 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 1213 0.14403541395041763 No Hit NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 1073 0.12741137606661013 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 991 0.11767443959180862 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 984 0.11684323769761826 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 847 0.10057542919703522 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 1.1874312774148197E-4 0.0 10-11 0.0 0.0 0.0 2.3748625548296394E-4 0.0 12-13 0.0 0.0 0.0 2.3748625548296394E-4 0.0 14-15 0.0 0.0 0.0 2.3748625548296394E-4 0.0 16-17 0.0 0.0 0.0 2.3748625548296394E-4 0.0 18-19 0.0 0.0 0.0 2.3748625548296394E-4 0.0 20-21 0.0 0.0 0.0 2.968578193537049E-4 0.0 22-23 0.0 0.0 0.0 5.937156387074099E-4 5.9371563870740984E-5 24-25 0.0 0.0 0.0 9.499450219318557E-4 1.1874312774148197E-4 26-27 0.0 0.0 0.0 0.0010686881496733377 2.3748625548296394E-4 28-29 0.0 0.0 0.0 0.0021373762993466753 2.3748625548296394E-4 30-31 0.0 0.0 0.0 0.004512238854176315 2.3748625548296394E-4 32-33 0.0 0.0 0.0 0.008193275814162255 2.3748625548296394E-4 34-35 0.0 0.0 0.0 0.011339968699311528 2.3748625548296394E-4 36-37 0.0 0.0 0.0 0.015911579117358583 2.3748625548296394E-4 38-39 0.0 0.0 0.0 0.020067588588310453 3.562293832244459E-4 40-41 0.0 0.0 0.0 0.026835946869574925 3.562293832244459E-4 42-43 0.0 0.0 0.0 0.03562293832244459 3.562293832244459E-4 44-45 0.0 0.0 0.0 0.05165326056754466 3.562293832244459E-4 46-47 0.0 1.1874312774148197E-4 0.0 0.08240773065258848 3.562293832244459E-4 48-49 0.0 1.1874312774148197E-4 0.0 0.13275481681497683 3.562293832244459E-4 50-51 0.0 1.1874312774148197E-4 0.0 0.1667747229129114 3.562293832244459E-4 52-53 0.0 1.1874312774148197E-4 0.0 0.19651987641215266 3.562293832244459E-4 54-55 0.0 1.1874312774148197E-4 0.0 0.23997986116553505 3.562293832244459E-4 56-57 0.0 1.1874312774148197E-4 0.0 0.27566217105185037 3.562293832244459E-4 58-59 0.0 1.1874312774148197E-4 0.0 0.32013147239103534 3.562293832244459E-4 60-61 0.0 1.1874312774148197E-4 0.0 0.38051235284757895 3.562293832244459E-4 62-63 0.0 1.1874312774148197E-4 0.0 0.4353716778641436 3.562293832244459E-4 64-65 0.0 1.1874312774148197E-4 0.0 0.49539632893746277 3.562293832244459E-4 66-67 0.0 1.1874312774148197E-4 0.0 0.585878592276472 3.562293832244459E-4 68-69 0.0 1.1874312774148197E-4 0.0 0.6831885854606164 3.562293832244459E-4 70-71 0.0 1.1874312774148197E-4 0.0 0.7825172118163661 4.7497251096592787E-4 72-73 0.0 1.1874312774148197E-4 0.0 0.8964512428843181 4.7497251096592787E-4 74-75 0.0 1.1874312774148197E-4 0.0 1.00397314505423 4.7497251096592787E-4 76-77 0.0 1.1874312774148197E-4 0.0 1.0948116377764636 4.7497251096592787E-4 78-79 0.0 1.1874312774148197E-4 0.0 1.2101112148134425 4.7497251096592787E-4 80-81 0.0 1.1874312774148197E-4 0.0 1.3520686240283843 5.343440748366688E-4 82-83 0.0 1.1874312774148197E-4 0.0 1.5093438967219772 5.937156387074099E-4 84-85 0.0 1.1874312774148197E-4 0.0 1.6691127750981412 5.937156387074099E-4 86-87 0.0 1.1874312774148197E-4 0.0 1.9225106096984637 5.937156387074099E-4 88-89 0.0 1.1874312774148197E-4 0.0 2.2913861360273775 5.937156387074099E-4 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TATAGCG 70 0.008348369 27.170752 5 TACCTGG 700 0.0 27.167515 2 GTACGCC 70 0.008387257 27.14488 9 GTACCTG 760 0.0 24.398598 1 GTACATG 2990 0.0 24.32955 1 TACATGG 2920 0.0 23.934395 2 ACATGGG 2940 0.0 23.609865 3 GTAAGCG 85 3.1532672E-6 22.350618 94-95 CATGGGG 1615 0.0 22.081493 4 GTATAGG 260 6.166374E-10 21.944302 1 GAGTACT 2925 0.0 21.761011 12-13 GCCGCGC 110 0.002875485 21.592518 8 GTACTTT 3100 0.0 20.455954 14-15 AGAGTAC 4425 0.0 19.966402 10-11 GTGTAGC 525 0.0 19.924032 1 AGTACTT 3040 0.0 19.844048 12-13 GTGTAGG 295 2.9758667E-9 19.340738 1 TATAGGG 230 2.0387306E-6 18.603844 2 GTGTATA 365 1.1459633E-10 18.236814 1 TATTAGG 290 5.1541065E-8 18.033611 2 >>END_MODULE