FastQCFastQC Report
Thu 26 May 2016
SRR937687_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937687_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences842154
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT64790.7693367246370616No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT44580.5293568634715266No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT39760.4721226759001323No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT25350.30101382882465677No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT19880.23606133795006615No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT17570.2086316754417838No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT15300.18167698544446742No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC14670.17419616839675403No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC14200.16861524139290437No Hit
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT13520.16054070870648363No Hit
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA13060.15507852483037543No Hit
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG12500.14842890967685246No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC11750.1395231750962413No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10560.12539274289500496No Hit
GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA8520.10116914483574264No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGCGGA300.00395215931.66553926-27
TAAGGTG3350.024.1079145
GTACATG30850.023.5678921
GTAAGGT2653.092282E-1123.305214
TACATGG31550.022.5864182
GAGTACT40050.022.29633312-13
ACATGGG31400.021.7865453
GGGTACG1100.002869406721.6003991
GTCTAGA4500.021.120391
GTACTTT42000.020.9784214-15
AGTACTT42050.020.3886912-13
AGAGTAC56250.020.18150310-11
ACGACCC1200.00476344319.7909628
TACCTGG6250.019.7628172
CATGGGG17450.019.3294014
GTACCTG6550.018.863251
CCCTTAT2051.5825683E-518.5447331
ATGGGGA10750.018.1188625
AGGTAAG3156.7848305E-918.0978162
GTATAGC3208.216375E-917.820331
Produced by FastQC (version 0.11.5)