##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937687_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 842154 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.296284290046714 33.0 31.0 34.0 31.0 34.0 2 32.59538635451473 34.0 31.0 34.0 31.0 34.0 3 32.69210144462889 34.0 31.0 34.0 31.0 34.0 4 36.27125086385625 37.0 35.0 37.0 35.0 37.0 5 36.20036952861353 37.0 35.0 37.0 35.0 37.0 6 36.25842304376634 37.0 37.0 37.0 35.0 37.0 7 36.21659815188196 37.0 36.0 37.0 35.0 37.0 8 36.19606152793907 37.0 36.0 37.0 35.0 37.0 9 37.92352230114682 39.0 38.0 39.0 35.0 39.0 10-11 37.839801271501415 39.0 38.0 39.0 35.0 39.0 12-13 37.74695720735163 39.0 37.0 39.0 35.0 39.0 14-15 38.99712582259302 40.0 38.0 41.0 36.0 41.0 16-17 38.96406357982032 40.0 38.0 41.0 36.0 41.0 18-19 38.92796745013382 40.0 38.0 41.0 35.5 41.0 20-21 38.832845892793955 40.0 38.0 41.0 35.0 41.0 22-23 38.7755202730142 40.0 38.0 41.0 35.0 41.0 24-25 38.79151853461481 40.0 38.0 41.0 35.0 41.0 26-27 38.651459234296816 40.0 38.0 41.0 34.5 41.0 28-29 38.5076524008673 40.0 38.0 41.0 34.0 41.0 30-31 38.589677184932924 40.0 38.0 41.0 35.0 41.0 32-33 38.515294708568746 40.0 38.0 41.0 35.0 41.0 34-35 38.36333497198849 40.0 38.0 41.0 34.0 41.0 36-37 38.21305723181271 40.0 38.0 41.0 34.0 41.0 38-39 38.077545793287214 40.0 38.0 41.0 34.0 41.0 40-41 37.92354901835056 40.0 37.5 41.0 33.0 41.0 42-43 37.70819648187861 40.0 37.0 41.0 33.0 41.0 44-45 37.411396846657496 40.0 37.0 41.0 33.0 41.0 46-47 37.035279176967634 40.0 37.0 41.0 31.5 41.0 48-49 36.67848873246461 40.0 36.0 41.0 31.0 41.0 50-51 36.39428121222484 39.0 36.0 41.0 30.0 41.0 52-53 36.183285361109725 39.0 36.0 41.0 30.0 41.0 54-55 35.96515898517373 39.0 35.0 40.0 29.5 41.0 56-57 35.65706687850441 39.0 35.0 40.0 28.5 41.0 58-59 35.37899897168451 38.0 35.0 40.0 28.0 41.0 60-61 35.008266896553366 38.0 34.0 40.0 27.5 41.0 62-63 34.64458460091622 37.5 34.0 40.0 26.5 41.0 64-65 34.2253311152117 37.0 34.0 39.5 26.0 41.0 66-67 33.78425798606905 36.5 33.0 39.0 26.0 40.5 68-69 33.31882114197641 36.0 33.0 39.0 25.0 40.0 70-71 32.87822892250111 35.0 32.5 38.5 24.0 40.0 72-73 32.36733424053083 35.0 32.0 37.5 22.5 39.5 74-75 31.888245499041744 35.0 31.0 37.0 21.5 39.0 76-77 30.707211507633996 34.0 30.0 35.5 20.5 38.0 78-79 31.273169752800555 34.0 31.0 36.0 20.5 38.5 80-81 31.05309183356013 34.0 31.0 36.0 20.0 37.0 82-83 30.66943872498379 34.0 31.0 35.0 18.5 37.0 84-85 30.2700183101903 34.0 30.5 35.0 17.0 36.5 86-87 29.923980055904266 34.0 30.0 35.0 10.0 36.0 88-89 29.48505914595193 34.0 29.5 35.0 7.0 36.0 90-91 28.934773212500325 34.0 29.0 35.0 2.0 35.0 92-93 28.48246817090461 34.0 29.0 35.0 2.0 35.0 94-95 27.974610344426317 33.0 28.0 35.0 2.0 35.0 96-97 27.369266784934823 33.0 27.0 35.0 2.0 35.0 98-99 26.469424238322205 32.5 25.0 34.5 2.0 35.0 100-101 23.65383172198909 29.5 12.5 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.19273271328197694 1101 2 0.16245846914646478 1101 3 0.18187177955142175 1101 4 0.06537513714830112 1101 5 -0.10585798390622614 1101 6 0.08279623313456597 1101 7 0.13060065113882757 1101 8 -0.02434792389370699 1101 9 -0.03970223919967708 1101 10-11 0.14616694122793916 1101 12-13 0.07302001892544041 1101 14-15 0.15948521193003984 1101 16-17 0.022128069224287117 1101 18-19 0.08893636584328135 1101 20-21 0.1533027624198482 1101 22-23 0.08321707015294066 1101 24-25 0.042400169748141536 1101 26-27 -0.03777068446900245 1101 28-29 -0.06114562626751052 1101 30-31 -0.10017802611030646 1101 32-33 0.05188998909480347 1101 34-35 -0.06385577865543013 1101 36-37 -0.24844124734678275 1101 38-39 -0.10349348702977323 1101 40-41 -0.012984666440338799 1101 42-43 -0.05831352996790429 1101 44-45 -0.21519884342043838 1101 46-47 -0.18972581341093786 1101 48-49 -0.07918493994709053 1101 50-51 0.017854862672031402 1101 52-53 -0.01648090777683109 1101 54-55 -0.14760176291891725 1101 56-57 -0.3163294031653052 1101 58-59 -0.577975687372124 1101 60-61 -0.1775935287885062 1101 62-63 -0.4463698341692819 1101 64-65 -0.2134493398699533 1101 66-67 -0.4242216805734458 1101 68-69 -0.7040766366931805 1101 70-71 -0.6854466383805473 1101 72-73 -0.6752588923386895 1101 74-75 -0.752947288059616 1101 76-77 -0.6473414213257804 1101 78-79 -0.5099953808506541 1101 80-81 -0.375390767346353 1101 82-83 -0.6078333036088885 1101 84-85 -0.5567405793553313 1101 86-87 -0.7499832833723268 1101 88-89 -0.7582393683396234 1101 90-91 -0.7196509296319178 1101 92-93 -0.8553573541404731 1101 94-95 -0.9191655577473377 1101 96-97 -0.853150946824595 1101 98-99 -1.0924417283532648 1101 100-101 -0.9326783206407754 1102 1 0.26068103735020287 1102 2 0.17578418943869423 1102 3 0.15257219865512894 1102 4 0.08081086781668745 1102 5 0.0994883418353254 1102 6 0.010448189628156968 1102 7 0.12889061525705614 1102 8 0.09386289551297722 1102 9 0.14388991820864305 1102 10-11 0.11211065882661586 1102 12-13 0.11876492794573323 1102 14-15 0.18312138585518767 1102 16-17 0.14786063120583748 1102 18-19 0.18908988262385407 1102 20-21 0.1316065614046451 1102 22-23 0.15452508336176152 1102 24-25 0.05289563573774814 1102 26-27 0.029572847247813172 1102 28-29 -0.1251367147584901 1102 30-31 -0.022324536574366505 1102 32-33 0.05246338248227289 1102 34-35 0.09392932876767901 1102 36-37 0.0783026511099294 1102 38-39 0.09292732091869738 1102 40-41 0.2420140486174418 1102 42-43 0.29310898801645635 1102 44-45 0.20724040199965543 1102 46-47 -0.11013861800503832 1102 48-49 -0.1966639219636903 1102 50-51 0.001973749776809086 1102 52-53 0.010913480600336811 1102 54-55 -0.19025798001038652 1102 56-57 0.00748714573109055 1102 58-59 -0.15287236062774667 1102 60-61 -0.38858683742776634 1102 62-63 -0.38138653841809145 1102 64-65 -0.3446999074666124 1102 66-67 -0.43183906922305226 1102 68-69 -0.5567831307718194 1102 70-71 -0.5598604438770138 1102 72-73 -0.6364681292375955 1102 74-75 -0.4683050526852526 1102 76-77 -0.48454842068620607 1102 78-79 -0.5427015078029456 1102 80-81 -0.3910404940304488 1102 82-83 -0.2429618847814936 1102 84-85 -0.10917684911976622 1102 86-87 -0.42560155312517267 1102 88-89 -0.5017315707692234 1102 90-91 -0.47354729952524366 1102 92-93 -0.25032926897489105 1102 94-95 -0.365608586501061 1102 96-97 -0.1923790733402022 1102 98-99 -0.1898891375039895 1102 100-101 -0.3821960325547984 1103 1 0.2178697302180055 1103 2 0.16992514115361956 1103 3 0.21639470694078966 1103 4 0.12898370596384723 1103 5 0.07891961808044101 1103 6 0.05755908201873439 1103 7 0.05865758075784555 1103 8 0.14238216108683588 1103 9 0.0724880958492875 1103 10-11 0.20884593107432892 1103 12-13 0.1895294749298273 1103 14-15 0.22005637729162686 1103 16-17 0.11244409269573197 1103 18-19 0.05474888795625077 1103 20-21 0.13045881820126937 1103 22-23 0.011762988350000114 1103 24-25 0.09156438312740534 1103 26-27 0.04041408311956474 1103 28-29 0.14895590979080708 1103 30-31 0.1269813674771072 1103 32-33 0.07058337156064454 1103 34-35 0.22468086044096225 1103 36-37 0.33142076775344975 1103 38-39 0.1440599011770871 1103 40-41 0.16956161223975386 1103 42-43 0.3149182673071138 1103 44-45 0.31807073781841666 1103 46-47 0.25538062922219495 1103 48-49 0.12840155929523434 1103 50-51 -0.10326410340918812 1103 52-53 0.012798326396968207 1103 54-55 -0.10240786976876137 1103 56-57 -0.06427999028538522 1103 58-59 -0.059844725216173345 1103 60-61 -0.00395418540171022 1103 62-63 -0.14378103184082391 1103 64-65 0.018438055907054718 1103 66-67 -0.036991124962256094 1103 68-69 -0.0635433220515651 1103 70-71 -0.1436840097915706 1103 72-73 -0.08740116298135092 1103 74-75 -0.24106970543222417 1103 76-77 -0.15431242914292298 1103 78-79 -0.2339688386173968 1103 80-81 -0.03378801966446332 1103 82-83 0.10062936970668801 1103 84-85 0.020739911168639935 1103 86-87 0.025107004279796286 1103 88-89 -0.26489662239338685 1103 90-91 -0.3234783917645103 1103 92-93 -0.20598386032542848 1103 94-95 -0.5446036447839653 1103 96-97 -0.5106336403005223 1103 98-99 -0.24079886024333064 1103 100-101 -0.417019003822908 1104 1 0.30335873396787605 1104 2 0.23841201659701738 1104 3 0.24244517820864786 1104 4 0.08647838519765827 1104 5 0.07890340241205251 1104 6 0.1801252115413874 1104 7 0.057234655856660765 1104 8 0.18160380900413742 1104 9 0.17381980761577864 1104 10-11 0.14473525913756902 1104 12-13 0.23319421598586132 1104 14-15 0.1532032304384785 1104 16-17 0.14451057693613478 1104 18-19 0.2581379626496698 1104 20-21 0.32256786857119124 1104 22-23 0.3604789101093928 1104 24-25 0.2125271884380382 1104 26-27 0.23266299362934006 1104 28-29 0.19385709556155462 1104 30-31 0.28702393360662626 1104 32-33 0.1816323226095875 1104 34-35 0.198056759903821 1104 36-37 0.2054128626151126 1104 38-39 0.2257625463579913 1104 40-41 0.1744749597618025 1104 42-43 0.17122920274848497 1104 44-45 -0.11370183743241569 1104 46-47 -0.22346197744649743 1104 48-49 -0.2476316828249665 1104 50-51 -0.44796259332506594 1104 52-53 -0.1647044606710395 1104 54-55 -0.29230145444496003 1104 56-57 -0.2058285866165832 1104 58-59 -0.33710833117542904 1104 60-61 -0.1950821878340605 1104 62-63 -0.36424317838993403 1104 64-65 -0.3243003650922347 1104 66-67 -0.47478782101982375 1104 68-69 -0.3408231436792093 1104 70-71 -0.4352924013999626 1104 72-73 -0.45776297508229646 1104 74-75 -0.43692660215868884 1104 76-77 -0.5707328203459205 1104 78-79 -0.654803945539463 1104 80-81 -0.6627451494911583 1104 82-83 -0.7110378036996181 1104 84-85 -0.7658314884462349 1104 86-87 -0.7235035156350733 1104 88-89 -0.5252635018907021 1104 90-91 -0.07344697390700716 1104 92-93 -0.2216502229483126 1104 94-95 -0.4340594064136418 1104 96-97 -0.28217108835970706 1104 98-99 -0.21301331245778599 1104 100-101 -0.3557176964346418 1105 1 0.24646458471915622 1105 2 0.21628157086546196 1105 3 0.2708544789725238 1105 4 0.10390472616970925 1105 5 0.08110603818808926 1105 6 0.16308164568895478 1105 7 0.0589185306153297 1105 8 0.05896467528467042 1105 9 0.1556245587280074 1105 10-11 0.22940075061746512 1105 12-13 0.18801164232684187 1105 14-15 0.018053394125210787 1105 16-17 0.10458780349535601 1105 18-19 0.25568182229879 1105 20-21 0.008829935221065455 1105 22-23 0.08586965971066718 1105 24-25 0.17988973355681281 1105 26-27 0.003805443054339719 1105 28-29 0.0758297304981781 1105 30-31 0.18195300269081827 1105 32-33 0.38528967172400286 1105 34-35 0.36944935622716457 1105 36-37 0.26775903906101206 1105 38-39 0.390709243243748 1105 40-41 0.4034290435358301 1105 42-43 0.43328913727379614 1105 44-45 0.44650130199509874 1105 46-47 0.4236554572886675 1105 48-49 0.47582000860456475 1105 50-51 0.3820516768000033 1105 52-53 0.4826454931609092 1105 54-55 0.4303351610171475 1105 56-57 0.7068499998016122 1105 58-59 0.8181959582562897 1105 60-61 0.44338335401099016 1105 62-63 0.4372300021691231 1105 64-65 0.634437987967452 1105 66-67 0.37368527559731746 1105 68-69 0.4568038184629941 1105 70-71 0.2543911375910568 1105 72-73 0.12597244001046448 1105 74-75 0.1767542506911397 1105 76-77 0.3076336700427298 1105 78-79 0.42803161225454645 1105 80-81 0.17532082885183087 1105 82-83 0.1938417396563814 1105 84-85 0.08822710300847447 1105 86-87 0.13138694016950936 1105 88-89 0.11698110484143953 1105 90-91 0.1635134894523489 1105 92-93 0.1960757906502799 1105 94-95 -0.03880716096749026 1105 96-97 0.07039669473438792 1105 98-99 0.46752559461851817 1105 100-101 0.5123222599155604 1106 1 0.1448738854830296 1106 2 0.11560898629398508 1106 3 0.18305123881471275 1106 4 0.11223596095523902 1106 5 -0.1361723551637084 1106 6 -0.0949505460343687 1106 7 -0.06329564717363922 1106 8 -0.09301740311708073 1106 9 0.13109253488850925 1106 10-11 0.010682228834539842 1106 12-13 -0.25650275371110354 1106 14-15 0.03437747286271531 1106 16-17 0.04962421329977218 1106 18-19 0.10366826567997123 1106 20-21 0.15711332311663995 1106 22-23 0.15968345556393615 1106 24-25 -0.03057887216802868 1106 26-27 -0.22714761243125992 1106 28-29 -0.1231126014081454 1106 30-31 -0.10267303609034428 1106 32-33 0.0367838377611065 1106 34-35 -0.19531445221739574 1106 36-37 0.10291286261510635 1106 38-39 -0.07715412030866986 1106 40-41 -0.36211594932910884 1106 42-43 -0.3628238275545428 1106 44-45 -0.38699729197786326 1106 46-47 -0.2875528865374051 1106 48-49 -0.5404725919158793 1106 50-51 -0.3139474418099226 1106 52-53 -0.0770529455195259 1106 54-55 -0.11309690899040703 1106 56-57 -0.026017980555984366 1106 58-59 -0.20199469481179477 1106 60-61 0.1245011454992806 1106 62-63 -0.16784166323841276 1106 64-65 -0.11085339539526018 1106 66-67 -0.2784620634440671 1106 68-69 -0.09029284064890675 1106 70-71 -0.07790603776359717 1106 72-73 -0.11734630841562677 1106 74-75 0.0018612766291923322 1106 76-77 -0.4071343354974353 1106 78-79 -0.49112970311521664 1106 80-81 -0.7758512100972155 1106 82-83 -0.5472499249117391 1106 84-85 -0.7347708823856287 1106 86-87 -0.34024593987749796 1106 88-89 -0.29162713825433784 1106 90-91 -0.7155681860282179 1106 92-93 -0.670097192645283 1106 94-95 0.019236048131812566 1106 96-97 -0.005958967147588368 1106 98-99 -0.33411179730627083 1106 100-101 -0.47833133279828033 1107 1 0.11531846283550351 1107 2 0.08894431324772967 1107 3 0.17831639032986146 1107 4 0.00582647132205949 1107 5 0.041459223783661514 1107 6 -0.010221678410765378 1107 7 -0.09499421176694511 1107 8 -0.07183518621117457 1107 9 -0.11437197866811033 1107 10-11 0.02869957333215467 1107 12-13 -0.15335781750695787 1107 14-15 -0.010080262384491334 1107 16-17 0.10238039352305606 1107 18-19 -0.03379186191173744 1107 20-21 -0.03414963541605687 1107 22-23 0.08422492286856453 1107 24-25 -0.020162205501357278 1107 26-27 0.10967455662775194 1107 28-29 0.08703991055357818 1107 30-31 -6.993518798168452E-4 1107 32-33 -0.10207182412086979 1107 34-35 -0.2239976659335028 1107 36-37 -0.4051114595539431 1107 38-39 -0.10461624152079452 1107 40-41 -0.38755952987137476 1107 42-43 -0.11220082117495878 1107 44-45 -0.4774079777832867 1107 46-47 -0.3859878945118922 1107 48-49 -0.32010277532895515 1107 50-51 -0.239485719321884 1107 52-53 -0.3279500747061306 1107 54-55 -0.44353949272247206 1107 56-57 -0.4583594877330128 1107 58-59 -0.23867731044177987 1107 60-61 -0.27419801718014725 1107 62-63 -0.4309746377200767 1107 64-65 -0.23627703973338754 1107 66-67 -0.0963199183085024 1107 68-69 -0.04272007350376583 1107 70-71 0.13735512651072668 1107 72-73 -0.09559092564050786 1107 74-75 -0.06632054155262779 1107 76-77 -0.20565407233953792 1107 78-79 -0.004187916831327243 1107 80-81 0.11243128591554097 1107 82-83 -0.3233414320887711 1107 84-85 -0.17324277233778318 1107 86-87 -0.12129657783603065 1107 88-89 -0.12682949072642913 1107 90-91 -0.09293561027064356 1107 92-93 -0.011031203809555024 1107 94-95 -0.06070813049657531 1107 96-97 0.10658090526070296 1107 98-99 0.36178632869054184 1107 100-101 0.35507676130060517 1108 1 0.20793252947692764 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-0.4460845225909438 >>END_MODULE >>Per sequence quality scores pass #Quality Count 4 3.0 5 8.0 6 26.0 7 72.0 8 141.0 9 323.0 10 521.0 11 883.0 12 1556.0 13 2281.0 14 2874.0 15 4019.0 16 5694.0 17 8430.0 18 8059.0 19 4755.0 20 3992.0 21 3943.0 22 4007.0 23 4353.0 24 5039.0 25 5720.0 26 7132.0 27 8506.0 28 10752.0 29 13572.0 30 16677.0 31 22093.0 32 29309.0 33 39792.0 34 56606.0 35 84729.0 36 134088.0 37 197377.0 38 143463.0 39 11355.0 40 4.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.208233521022045 19.166576603004522 14.910148135919723 21.715041740053707 2 15.729545902530893 20.758673591765877 39.92523932677396 23.58654117892927 3 20.853311864575836 24.077187782757072 29.909137758652214 25.160362594014874 4 13.148426534814298 16.761779911987592 37.03408165252436 33.05571190067375 5 13.544949285579383 37.691404027457885 34.089427002474245 14.67421968448848 6 31.973249548182398 36.649947634280664 16.70715807322651 14.669644744310423 7 28.66542223868794 33.09727199538327 20.149521346452072 18.087784419476723 8 26.18012857505872 35.05783977752287 18.909368120320035 19.85266352709837 9 25.76998981183964 17.909907214119983 20.41538720946525 35.90471576457512 10-11 25.526863257788957 26.69772986888384 26.570674722200454 21.204732151126755 12-13 26.760428745391902 23.940619725066693 27.39922446791267 21.899727061628735 14-15 22.90810231857831 25.6032744604906 26.659732068006566 24.828891152924523 16-17 21.879549346081596 28.8446649900137 27.121405348665444 22.154380315239255 18-19 21.600087394942015 27.766536761684918 29.579506836041862 21.0538690073312 20-21 22.579421340989892 25.98230252424141 29.86454971418529 21.57372642058341 22-23 22.0581975053123 26.0088618469761 29.992358838899747 21.940581808811856 24-25 22.594271933256664 26.17996880608774 29.984034939081628 21.24172432157397 26-27 22.187502783063536 26.012189076141652 30.48967075245132 21.31063738834349 28-29 21.4354246521989 27.30927342937346 30.062411832599494 21.192890085828147 30-31 21.977215568648965 26.257252236526814 29.81396514176742 21.951567053056802 32-33 21.99597698283212 26.47983622947822 29.926830484685702 21.597356303003963 34-35 22.71247301562422 26.175853822460027 29.826433170180277 21.285239991735477 36-37 22.39441954796866 26.085134072865536 30.16823526338413 21.352211115781675 38-39 21.85176345419009 26.78744030189253 30.258539412031528 21.102256831885853 40-41 22.030345040506564 26.76811366179915 29.709403637723796 21.492137659970492 42-43 21.672232342832984 26.995568043360024 29.173307080442623 22.15889253336437 44-45 22.369246004887465 26.360796243917385 29.166340123065375 22.103617628129772 46-47 22.905311855076388 27.092491397060396 28.74587070773279 21.25632604013043 48-49 22.75153950465117 27.08429812124623 28.630333644440327 21.53382872966227 50-51 21.883645983988675 27.474784896824094 29.14176029562289 21.499808823564337 52-53 22.57627464810474 27.84621340039945 27.88599234819285 21.691519603302957 54-55 22.034179062474397 27.44715033966512 28.20758223268218 22.3110883651783 56-57 22.60417928312399 27.465285446604774 28.186293718251054 21.744241552020178 58-59 23.126233444239414 27.1979946660587 27.988052066486652 21.687719823215232 60-61 22.298831330136768 27.44123996323713 29.106374843555926 21.153553863070172 62-63 22.006604492764982 27.511120293912988 29.00870862098856 21.47356659233347 64-65 21.924196762112395 28.143664935391865 28.297496657380954 21.634641645114787 66-67 22.19706846966232 27.58907515727527 27.92054659836562 22.29330977469679 68-69 21.97359390325285 27.574885353510165 28.25635216361853 22.195168579618453 70-71 22.728681452560934 27.550008668248328 27.980333763183456 21.740976116007285 72-73 22.471008865361917 27.1994195835916 28.54412613370001 21.785445417346473 74-75 21.863697138528106 28.54365116118905 27.697784490722597 21.894867209560246 76-77 22.277160709323947 27.64001595907637 27.777401757873264 22.30542157372642 78-79 22.442748000959444 27.88694229321478 27.651118441520197 22.01919126430558 80-81 22.80004607233356 27.72432357977282 27.630753995112535 21.844876352781085 82-83 22.602586009330913 27.60005082248111 28.052881504060455 21.74448166412752 84-85 22.218145374836432 28.063216466347008 27.93491451682234 21.78372364199422 86-87 22.153430370217322 28.11397915345649 27.68163542534976 22.050955050976427 88-89 21.986774390432153 28.19306207653232 27.21165012574897 22.608513407286555 90-91 22.46603697737263 27.51131012148249 27.451159691968048 22.57149320917683 92-93 22.74699516233604 27.71866264834197 27.618145158417462 21.916197030904527 94-95 22.53619884249199 27.59038133168043 27.742135048934042 22.131284776893537 96-97 21.784317357632926 27.74777534750176 28.362508519819414 22.105398775045895 98-99 22.12386228024865 28.192116559499848 27.197988469919075 22.486032690332426 100-101 21.66658551039968 28.03608563926 27.38430184430586 22.913027006034465 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 233.0 1 177.0 2 158.5 3 204.0 4 178.5 5 158.0 6 275.0 7 612.5 8 1070.5 9 1325.5 10 1298.0 11 1229.5 12 1279.5 13 1385.5 14 1479.0 15 1525.0 16 1567.0 17 1611.5 18 1668.0 19 1770.0 20 1876.0 21 2024.5 22 2416.5 23 2954.5 24 3421.5 25 4168.5 26 5109.0 27 6097.5 28 7135.0 29 8347.0 30 9695.0 31 10844.5 32 12177.5 33 13814.5 34 15623.0 35 16983.5 36 18822.0 37 20903.5 38 22462.0 39 23743.0 40 24550.0 41 25839.5 42 26808.5 43 27707.5 44 29016.5 45 30048.5 46 30897.5 47 35108.5 48 42692.5 49 41247.5 50 34052.0 51 30869.0 52 29016.0 53 28009.5 54 27066.0 55 25087.0 56 22275.0 57 19825.5 58 17861.0 59 15709.5 60 12887.5 61 10169.0 62 7867.5 63 5655.5 64 3993.0 65 2825.0 66 2036.0 67 1587.5 68 1163.5 69 808.0 70 596.0 71 373.5 72 252.5 73 175.0 74 115.5 75 89.5 76 64.0 77 34.5 78 18.0 79 13.0 80 12.0 81 8.5 82 5.5 83 4.5 84 1.0 85 0.5 86 0.5 87 1.0 88 1.5 89 0.5 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.01864267105541267 2 0.0 3 0.0 4 0.0 5 0.0336043051508394 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0026717203741833443 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 5.343440748366688E-4 24-25 2.968578193537049E-4 26-27 7.718303303196328E-4 28-29 7.124587664488918E-4 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 7.718303303196328E-4 42-43 0.010449395241250414 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 1.1874312774148197E-4 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 8.312018941903738E-4 84-85 0.0 86-87 0.0 88-89 0.0 90-91 0.011696198082535973 92-93 0.0014249175328977836 94-95 0.0 96-97 0.0 98-99 0.0010686881496733377 100-101 0.01864267105541267 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 842154.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 66.62309767793066 #Duplication Level Percentage of deduplicated Percentage of total 1 84.86331971016932 56.538572383240684 2 9.318991485421773 12.417201599861178 3 2.446807123747235 4.8904161001340585 4 1.0643265856233775 2.8363493630081877 5 0.580853321977248 1.934912380332035 6 0.3723832514119196 1.4885595439465038 7 0.24206604053243871 1.1289032624031814 8 0.1788027776674383 0.9529915937298449 9 0.13915990255017285 0.834413740240633 >10 0.7303676792583558 8.737760212772402 >50 0.04002617130068092 1.8027355073460978 >100 0.019831138115875318 2.3695333917876504 >500 5.408492160418867E-4 0.25427322388753465 >1k 0.0023436799361815086 3.035183924250355 >5k 1.8028307201396223E-4 0.7781937730596774 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 6479 0.7693367246370616 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4458 0.5293568634715266 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3976 0.4721226759001323 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2535 0.30101382882465677 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 1988 0.23606133795006615 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 1757 0.2086316754417838 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 1530 0.18167698544446742 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 1467 0.17419616839675403 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 1420 0.16861524139290437 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 1352 0.16054070870648363 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 1306 0.15507852483037543 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 1250 0.14842890967685246 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 1175 0.1395231750962413 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1056 0.12539274289500496 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 852 0.10116914483574264 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 1.1874312774148197E-4 0.0 4 0.0 0.0 0.0 1.1874312774148197E-4 0.0 5 0.0 0.0 0.0 1.1874312774148197E-4 0.0 6 1.1874312774148197E-4 0.0 0.0 1.1874312774148197E-4 0.0 7 1.1874312774148197E-4 0.0 0.0 1.1874312774148197E-4 0.0 8 1.1874312774148197E-4 0.0 0.0 1.1874312774148197E-4 0.0 9 1.1874312774148197E-4 0.0 0.0 1.1874312774148197E-4 0.0 10-11 1.1874312774148197E-4 0.0 0.0 1.1874312774148197E-4 0.0 12-13 1.1874312774148197E-4 0.0 0.0 1.1874312774148197E-4 0.0 14-15 1.1874312774148197E-4 0.0 0.0 1.1874312774148197E-4 0.0 16-17 1.1874312774148197E-4 0.0 0.0 1.1874312774148197E-4 0.0 18-19 1.1874312774148197E-4 0.0 0.0 1.1874312774148197E-4 0.0 20-21 1.1874312774148197E-4 0.0 0.0 1.7811469161222295E-4 0.0 22-23 1.1874312774148197E-4 0.0 0.0 3.562293832244459E-4 0.0 24-25 1.1874312774148197E-4 0.0 0.0 7.124587664488918E-4 0.0 26-27 1.1874312774148197E-4 0.0 0.0 8.312018941903738E-4 0.0 28-29 1.1874312774148197E-4 0.0 0.0 0.0018998900438637115 0.0 30-31 1.1874312774148197E-4 0.0 0.0 0.004274752598693351 0.0 32-33 1.1874312774148197E-4 0.0 0.0 0.007955789558679292 0.0 34-35 1.1874312774148197E-4 0.0 0.0 0.011102482443828565 0.0 36-37 1.1874312774148197E-4 0.0 0.0 0.01561472129800488 0.0 38-39 1.1874312774148197E-4 0.0 0.0 0.020423817971534898 5.9371563870740984E-5 40-41 1.1874312774148197E-4 0.0 0.0 0.027429662508282333 1.1874312774148197E-4 42-43 1.1874312774148197E-4 0.0 0.0 0.03591979614179829 1.1874312774148197E-4 44-45 1.1874312774148197E-4 0.0 0.0 0.05165326056754466 1.1874312774148197E-4 46-47 1.1874312774148197E-4 0.0 0.0 0.08282333159968366 1.1874312774148197E-4 48-49 1.1874312774148197E-4 0.0 0.0 0.13340790401755498 1.1874312774148197E-4 50-51 1.1874312774148197E-4 0.0 0.0 0.16730906698774808 1.1874312774148197E-4 52-53 1.1874312774148197E-4 0.0 0.0 0.19752919299795524 1.1874312774148197E-4 54-55 1.1874312774148197E-4 0.0 0.0 0.24116729244294988 1.1874312774148197E-4 56-57 1.1874312774148197E-4 0.0 0.0 0.27720583171248964 1.1874312774148197E-4 58-59 1.1874312774148197E-4 0.0 0.0 0.3216157614878039 1.1874312774148197E-4 60-61 1.1874312774148197E-4 0.0 0.0 0.38199664194434746 1.1874312774148197E-4 62-63 1.1874312774148197E-4 0.0 0.0 0.43715282478026585 1.1874312774148197E-4 64-65 1.1874312774148197E-4 0.0 0.0 0.49717747585358496 1.1874312774148197E-4 66-67 1.1874312774148197E-4 0.0 0.0 0.5887284273422675 1.1874312774148197E-4 68-69 1.1874312774148197E-4 0.0 0.0 0.6871071086760854 1.1874312774148197E-4 70-71 1.1874312774148197E-4 0.0 0.0 0.786673221287318 1.1874312774148197E-4 72-73 1.1874312774148197E-4 0.0 0.0 0.90060725235527 2.3748625548296394E-4 74-75 1.1874312774148197E-4 0.0 0.0 1.0089009848555015 2.3748625548296394E-4 76-77 1.1874312774148197E-4 0.0 0.0 1.1002144500887012 2.3748625548296394E-4 78-79 1.1874312774148197E-4 0.0 0.0 1.2174732887334145 2.3748625548296394E-4 80-81 1.1874312774148197E-4 0.0 0.0 1.361033730172866 2.3748625548296394E-4 82-83 1.1874312774148197E-4 0.0 0.0 1.5209213516767717 2.3748625548296394E-4 84-85 1.1874312774148197E-4 0.0 0.0 1.6818776613303505 2.3748625548296394E-4 86-87 1.1874312774148197E-4 0.0 0.0 1.941687624828713 2.3748625548296394E-4 88-89 1.1874312774148197E-4 0.0 0.0 2.316915908491796 2.3748625548296394E-4 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TAGCGGA 30 0.003952159 31.665539 26-27 TAAGGTG 335 0.0 24.107914 5 GTACATG 3085 0.0 23.567892 1 GTAAGGT 265 3.092282E-11 23.30521 4 TACATGG 3155 0.0 22.586418 2 GAGTACT 4005 0.0 22.296333 12-13 ACATGGG 3140 0.0 21.786545 3 GGGTACG 110 0.0028694067 21.600399 1 GTCTAGA 450 0.0 21.12039 1 GTACTTT 4200 0.0 20.97842 14-15 AGTACTT 4205 0.0 20.38869 12-13 AGAGTAC 5625 0.0 20.181503 10-11 ACGACCC 120 0.004763443 19.790962 8 TACCTGG 625 0.0 19.762817 2 CATGGGG 1745 0.0 19.329401 4 GTACCTG 655 0.0 18.86325 1 CCCTTAT 205 1.5825683E-5 18.544733 1 ATGGGGA 1075 0.0 18.118862 5 AGGTAAG 315 6.7848305E-9 18.097816 2 GTATAGC 320 8.216375E-9 17.82033 1 >>END_MODULE