FastQCFastQC Report
Thu 26 May 2016
SRR937686_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937686_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences820957
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT41410.5044113150871483No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT32370.3942959253651531No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT29560.3600675796661701No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT25670.31268385554907263No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC19630.23911118365517314No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT19390.2361877662289255No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16180.1970870581528631No Hit
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT13760.16760926577153248No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT11650.14190755423243848No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN10320.12570694932864937No Hit
ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC10080.12278353190240171No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC9090.11072443501913011No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG28250.027.0957241
TACATGG28550.026.1401062
ACATGGG28250.024.5652793
GTATAGA2202.1427695E-923.7718721
CGTATAT500.001655952123.74505490-91
GAGTACT27650.022.85100712-13
TCTAGAC3400.022.3680633
CCTACAC3550.021.4229343
CATGGGG15450.021.2279434
GTACTTT29750.021.07830814-15
GTCTAGG3001.6552804E-1020.6022871
AGTACTT29450.020.2445112-13
AGAGTAC40550.020.20910510-11
CTAGACT4350.019.6684684
TAGACTC2952.9867806E-919.3351065
TACAGCG1507.6834473E-419.0128545
AGGATCG1250.006006193319.0128525
GTACAAG5550.018.8461671
ACTTTTT34900.018.71656216-17
GATCCCG650.007554936718.27322444-45
Produced by FastQC (version 0.11.5)