##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937686_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 820957 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.72919288099133 33.0 31.0 34.0 30.0 34.0 2 31.86840723691984 33.0 31.0 34.0 30.0 34.0 3 31.915533943921545 34.0 31.0 34.0 30.0 34.0 4 35.47609436304216 37.0 35.0 37.0 33.0 37.0 5 35.458948519837215 37.0 35.0 37.0 33.0 37.0 6 35.53304497068665 37.0 35.0 37.0 33.0 37.0 7 35.47448404727653 37.0 35.0 37.0 33.0 37.0 8 35.46574181108146 37.0 35.0 37.0 33.0 37.0 9 37.08226131210283 39.0 37.0 39.0 34.0 39.0 10-11 36.9635011334333 39.0 37.0 39.0 33.0 39.0 12-13 36.83787823235565 39.0 37.0 39.0 33.0 39.0 14-15 37.98654558034099 40.0 38.0 41.0 33.0 41.0 16-17 37.92399297405345 40.0 38.0 41.0 33.0 41.0 18-19 37.85237594660865 40.0 38.0 41.0 33.0 41.0 20-21 37.61876139676012 40.0 37.5 41.0 32.0 41.0 22-23 37.501610315765625 40.0 37.0 41.0 32.0 41.0 24-25 37.34713267564562 40.0 37.0 41.0 32.0 41.0 26-27 37.44092138808732 40.0 37.0 41.0 32.0 41.0 28-29 37.39451761785331 40.0 37.0 41.0 32.0 41.0 30-31 37.199856387118935 39.5 37.0 41.0 31.5 41.0 32-33 36.965582850258905 39.0 36.0 41.0 31.0 41.0 34-35 36.69301559034152 39.0 36.0 41.0 30.0 41.0 36-37 36.73670119141441 39.0 36.0 41.0 30.0 41.0 38-39 36.70300697844101 39.0 36.0 41.0 30.0 41.0 40-41 36.52473820187903 39.0 36.0 41.0 30.0 41.0 42-43 36.2879754968896 39.0 35.0 41.0 30.0 41.0 44-45 36.06901640402603 39.0 35.0 41.0 29.5 41.0 46-47 35.71962904269042 39.0 35.0 40.5 27.5 41.0 48-49 35.381138110765846 39.0 35.0 40.0 26.5 41.0 50-51 34.503096386290636 38.0 34.0 39.5 25.0 40.5 52-53 34.63921801020029 38.0 34.0 39.5 26.0 40.5 54-55 34.93131978897799 38.0 34.5 40.0 26.0 41.0 56-57 34.62800426818944 38.0 34.0 40.0 24.5 41.0 58-59 34.38523454943438 38.0 34.0 40.0 23.5 41.0 60-61 34.01029408361218 38.0 33.5 40.0 22.5 41.0 62-63 33.649005368125245 37.0 33.0 40.0 22.0 41.0 64-65 33.26620699987941 37.0 33.0 39.5 20.5 41.0 66-67 32.80525228483343 36.0 32.0 39.0 19.0 41.0 68-69 32.29543021132653 36.0 31.5 39.0 17.5 40.0 70-71 31.816345435875448 35.0 31.0 38.5 13.5 40.0 72-73 31.32604082796054 35.0 31.0 37.5 9.0 40.0 74-75 30.825869686232043 35.0 30.0 37.0 7.0 39.0 76-77 30.34894763063108 34.5 30.0 36.5 4.0 39.0 78-79 29.782251080141833 34.0 29.0 36.0 2.0 38.5 80-81 29.181840827229685 34.0 29.0 35.5 2.0 37.0 82-83 28.63452044382349 34.0 28.0 35.0 2.0 37.0 84-85 28.13773863917355 34.0 27.0 35.0 2.0 36.0 86-87 27.632128113896343 33.0 26.0 35.0 2.0 36.0 88-89 27.082130367363945 33.0 25.5 35.0 2.0 35.5 90-91 26.516823658242757 32.5 25.0 35.0 2.0 35.0 92-93 25.915347576060622 32.0 23.5 34.5 2.0 35.0 94-95 25.24028115967097 32.0 19.5 34.0 2.0 35.0 96-97 24.424022208227715 31.5 12.5 34.0 2.0 35.0 98-99 23.450073511767364 31.0 2.0 34.0 2.0 35.0 100-101 20.94032513274142 28.0 2.0 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.14134338786342227 1101 2 0.1446613490913542 1101 3 0.0794288504234224 1101 4 0.037823362825875506 1101 5 0.12953357066792393 1101 6 -0.02416432779623534 1101 7 -0.15210191870281164 1101 8 -0.025026479936578028 1101 9 -0.10075105985403354 1101 10-11 -0.13514706233181784 1101 12-13 -0.057892300807608876 1101 14-15 0.09676653307133876 1101 16-17 0.08733500619975842 1101 18-19 0.12006341886529981 1101 20-21 0.17917606611938197 1101 22-23 0.1923114583048502 1101 24-25 -0.03995555641295567 1101 26-27 0.1377181664629319 1101 28-29 0.07451034006390955 1101 30-31 -0.0982331626864621 1101 32-33 -0.0942807805371686 1101 34-35 0.00343155771610526 1101 36-37 -0.07315432454274884 1101 38-39 -0.13676336747560214 1101 40-41 -0.013968079249217169 1101 42-43 0.01917051597671815 1101 44-45 0.16945930075748805 1101 46-47 0.06435217199514653 1101 48-49 0.2833934763783148 1101 50-51 0.12855404482630917 1101 52-53 0.14430409268894095 1101 54-55 0.2301577894044442 1101 56-57 0.30409684946888405 1101 58-59 0.1906084487453512 1101 60-61 0.3952317051577623 1101 62-63 0.32576686751369266 1101 64-65 0.20342119595454733 1101 66-67 0.4602415515504745 1101 68-69 0.7244822559343405 1101 70-71 0.6751628755281516 1101 72-73 0.6088692295164471 1101 74-75 0.4990290103150947 1101 76-77 0.5585018372802502 1101 78-79 0.7121488260528466 1101 80-81 0.730997428736373 1101 82-83 0.7248827227579575 1101 84-85 0.9880057837926977 1101 86-87 0.9668784926481919 1101 88-89 0.7147178967588914 1101 90-91 0.7253249026669977 1101 92-93 1.1659473610249051 1101 94-95 1.0796439300781415 1101 96-97 1.3342950007429089 1101 98-99 1.6972188465509426 1101 100-101 1.7468421840729782 1102 1 0.17181304604787684 1102 2 0.16996378612885366 1102 3 0.13132404324283087 1102 4 0.18639137659720006 1102 5 -0.013987631885363783 1102 6 0.0365832246118174 1102 7 0.12360857420489424 1102 8 0.11312979133955992 1102 9 -0.007283224170478775 1102 10-11 0.0567945403392045 1102 12-13 0.2026391083453234 1102 14-15 0.2725635427427875 1102 16-17 0.1763060513282113 1102 18-19 0.20292477500813533 1102 20-21 0.2871815425413331 1102 22-23 0.1612761707629673 1102 24-25 0.23800779139929773 1102 26-27 0.33788629440071105 1102 28-29 0.29386972951124335 1102 30-31 0.139425562871061 1102 32-33 0.13503769926518316 1102 34-35 0.12505896479976997 1102 36-37 0.31802111421021806 1102 38-39 0.2838054728132633 1102 40-41 0.3099480084092008 1102 42-43 0.255827312002296 1102 44-45 0.27368524329965993 1102 46-47 0.22121583525169086 1102 48-49 0.21759390985405958 1102 50-51 0.030519551098024067 1102 52-53 0.19197007257304932 1102 54-55 0.12845822555596698 1102 56-57 0.08684017415053802 1102 58-59 0.1684455891135883 1102 60-61 0.18967040163853 1102 62-63 0.1563173936010287 1102 64-65 -0.046942263645689764 1102 66-67 -0.029408397461125446 1102 68-69 -0.07170089797333645 1102 70-71 0.06227633735819893 1102 72-73 0.12456488673167598 1102 74-75 -0.05216899415047749 1102 76-77 -0.02622587276074384 1102 78-79 0.2673619813578725 1102 80-81 0.311989457896388 1102 82-83 0.1721476335208223 1102 84-85 0.25630437663172145 1102 86-87 0.5756971077258939 1102 88-89 0.36729957482733866 1102 90-91 0.7828644967220164 1102 92-93 0.7694664526880786 1102 94-95 0.6223604672669723 1102 96-97 0.5150667243292801 1102 98-99 0.7725664744753438 1102 100-101 1.0175095821184748 1103 1 0.19371435590730357 1103 2 0.23719062560700266 1103 3 0.10778039666253392 1103 4 0.20240154443983016 1103 5 0.1562487634819263 1103 6 0.06170295653782887 1103 7 0.08269224794825192 1103 8 0.0856694680677208 1103 9 0.017300394653979367 1103 10-11 0.2201211787967381 1103 12-13 0.284673566034229 1103 14-15 0.2757719399302374 1103 16-17 0.14617693088495542 1103 18-19 0.06333181997015203 1103 20-21 0.24705282309324872 1103 22-23 0.1364924902201281 1103 24-25 -0.029110779384190266 1103 26-27 -0.02528195096649455 1103 28-29 0.06659874569575663 1103 30-31 0.11966478263907732 1103 32-33 0.12468679362631718 1103 34-35 0.15851242233700447 1103 36-37 0.32381157157135476 1103 38-39 0.39958018675552154 1103 40-41 0.4456421571923599 1103 42-43 0.42717690481785553 1103 44-45 0.5005434636133756 1103 46-47 0.730684987390994 1103 48-49 0.47959761295724235 1103 50-51 0.3754813083634403 1103 52-53 0.578768265653963 1103 54-55 0.5905027255585793 1103 56-57 0.7785197859872213 1103 58-59 0.6276726622150122 1103 60-61 0.7869588025918475 1103 62-63 0.948404451394893 1103 64-65 0.9369534218206752 1103 66-67 0.8164739052274612 1103 68-69 0.8398426195911242 1103 70-71 0.7552378261312072 1103 72-73 0.9491107597068691 1103 74-75 0.8157689143633498 1103 76-77 0.7951421135287049 1103 78-79 0.7975081005092264 1103 80-81 0.8775229645293692 1103 82-83 0.7493884555260379 1103 84-85 1.079105484773148 1103 86-87 0.9702558648500954 1103 88-89 0.8908467083221154 1103 90-91 0.6984833455869399 1103 92-93 0.8904724100814718 1103 94-95 0.9086147888616054 1103 96-97 0.9674097565152451 1103 98-99 1.0295568662824905 1103 100-101 1.1799261358962845 1104 1 -0.017733261469619066 1104 2 0.18656508239309133 1104 3 0.12068767182750406 1104 4 0.011634500765147493 1104 5 0.10224233661347171 1104 6 -0.015239485284887166 1104 7 0.01650757743063025 1104 8 0.3142447696190729 1104 9 0.25887182208629866 1104 10-11 0.25217692236901 1104 12-13 0.19361411237500192 1104 14-15 0.4562966603637406 1104 16-17 0.41386876015032925 1104 18-19 0.3067736688852065 1104 20-21 0.3036450336193255 1104 22-23 0.19643539793954545 1104 24-25 0.1095663929303825 1104 26-27 0.06173365600350422 1104 28-29 0.17127286291263744 1104 30-31 0.1702755174003272 1104 32-33 0.2965115481192626 1104 34-35 0.31563010511032275 1104 36-37 0.00877085402208877 1104 38-39 -0.023098616767072144 1104 40-41 0.28566245616362806 1104 42-43 0.2265121192043864 1104 44-45 0.30891809924001024 1104 46-47 0.48115173842787584 1104 48-49 0.30207843885882113 1104 50-51 0.38477589063408146 1104 52-53 0.1009211412846085 1104 54-55 0.35329808299476184 1104 56-57 0.4449212294696281 1104 58-59 0.3826838995523616 1104 60-61 0.5079635674962688 1104 62-63 0.3421310212902142 1104 64-65 0.4270647927758304 1104 66-67 0.3120604167774914 1104 68-69 0.5613656803388096 1104 70-71 0.44021875170506775 1104 72-73 0.47068325790422705 1104 74-75 0.5227358356974285 1104 76-77 0.5668259598989813 1104 78-79 0.46546614018904364 1104 80-81 0.5374850093260761 1104 82-83 0.8632187529367243 1104 84-85 0.7816873954508381 1104 86-87 0.3136866643812368 1104 88-89 0.7809748242198999 1104 90-91 0.5569868717073412 1104 92-93 0.5448146470918971 1104 94-95 0.6079297270590978 1104 96-97 0.5644353079307614 1104 98-99 0.9558916489456664 1104 100-101 1.3481370583831485 1105 1 0.19531044619234095 1105 2 0.2856203449878798 1105 3 0.3059652452329864 1105 4 0.1655566412710172 1105 5 0.293313498233033 1105 6 0.14092065400745213 1105 7 0.14930429913614063 1105 8 0.2873829217091952 1105 9 0.22853645936048395 1105 10-11 0.41829447080035465 1105 12-13 0.3505415027247949 1105 14-15 0.4835410345887965 1105 16-17 0.5483377520689103 1105 18-19 0.39897496859530435 1105 20-21 0.5884036316677026 1105 22-23 0.32018925617909844 1105 24-25 0.38293347456237115 1105 26-27 0.5322967171520219 1105 28-29 0.5016495560679033 1105 30-31 0.5000652305202422 1105 32-33 0.4073899516508135 1105 34-35 0.5114403427456367 1105 36-37 0.5291993714321634 1105 38-39 0.6251200710357594 1105 40-41 0.7924174898288712 1105 42-43 0.5281055391655158 1105 44-45 0.7393913766258109 1105 46-47 0.8324039625032853 1105 48-49 0.8382550223858587 1105 50-51 0.9196107008218846 1105 52-53 0.8888681914351295 1105 54-55 1.070550312932646 1105 56-57 0.9105715388616034 1105 58-59 0.9848675239085907 1105 60-61 0.8262357210461388 1105 62-63 1.1451350986396704 1105 64-65 1.2582805522737743 1105 66-67 1.0616471124829374 1105 68-69 1.0956420900435475 1105 70-71 1.007988931067402 1105 72-73 1.051989885722243 1105 74-75 1.216322730726933 1105 76-77 1.1007901929557065 1105 78-79 1.0841013273338156 1105 80-81 0.904587159974028 1105 82-83 1.1206765504645197 1105 84-85 1.1654844014862533 1105 86-87 1.1425867473352191 1105 88-89 1.0098889771073125 1105 90-91 1.0327242974371451 1105 92-93 1.1736879092352765 1105 94-95 1.1804265246836252 1105 96-97 1.421840929455417 1105 98-99 1.4416165289848237 1105 100-101 1.2077858083574995 1106 1 0.015813272505514675 1106 2 0.06811569489596536 1106 3 0.15984539795179487 1106 4 -0.009304943712358238 1106 5 0.08611156640537843 1106 6 0.17827186013070673 1106 7 0.16476109588452204 1106 8 0.07723502521860581 1106 9 0.3083802597817993 1106 10-11 0.08695955903426977 1106 12-13 -0.06708116724377078 1106 14-15 0.10182740773464616 1106 16-17 0.05720102667633142 1106 18-19 0.02274264891553912 1106 20-21 0.2600010878045609 1106 22-23 0.024776984287413484 1106 24-25 0.15878454109222417 1106 26-27 0.17681214675525325 1106 28-29 0.1382281036438755 1106 30-31 0.4978507715443925 1106 32-33 0.6363521381548551 1106 34-35 0.53531897262733 1106 36-37 0.7760286070041928 1106 38-39 0.7927363958496656 1106 40-41 0.47054954881343036 1106 42-43 0.8084380977856824 1106 44-45 0.760380241785839 1106 46-47 0.952402620956299 1106 48-49 1.1183002361450534 1106 50-51 0.8586265129472963 1106 52-53 0.8180762073596455 1106 54-55 0.836938940965176 1106 56-57 1.018689348441697 1106 58-59 0.9699573169115183 1106 60-61 1.0121940750767706 1106 62-63 1.0566791104452875 1106 64-65 1.1951507956738965 1106 66-67 1.3103291332152693 1106 68-69 1.3294066521734962 1106 70-71 1.0351715736248934 1106 72-73 0.7410754945488947 1106 74-75 1.0931805013659108 1106 76-77 1.353582967456159 1106 78-79 0.9876141862429577 1106 80-81 1.0620140728568686 1106 82-83 0.7280057479656179 1106 84-85 0.9060310274955832 1106 86-87 0.7995830961519381 1106 88-89 1.0747446157743248 1106 90-91 1.2796405522730616 1106 92-93 1.4699866898778389 1106 94-95 1.350483921139766 1106 96-97 1.2381305730752707 1106 98-99 1.4263392094682175 1106 100-101 1.3659460398287457 1107 1 0.6223066364919951 1107 2 0.7019801055767481 1107 3 0.7465685534772568 1107 4 0.7328312665204066 1107 5 0.5633558878566021 1107 6 0.8592052644765715 1107 7 0.9084736771452455 1107 8 0.7237960473707687 1107 9 0.8694880551907858 1107 10-11 0.9303113681140545 1107 12-13 0.9329683452588 1107 14-15 1.0931613816081054 1107 16-17 1.070978884149909 1107 18-19 1.0170915327431516 1107 20-21 1.2353197743582953 1107 22-23 1.1463649706808425 1107 24-25 1.1283664682258845 1107 26-27 1.0751375536275916 1107 28-29 1.0924116424763994 1107 30-31 1.0425511941402945 1107 32-33 1.1860078255355901 1107 34-35 1.3376075207869391 1107 36-37 1.4613552423218579 1107 38-39 1.3026723507584919 1107 40-41 1.3330412855782043 1107 42-43 1.2586338728972208 1107 44-45 1.3769393637284395 1107 46-47 1.5536409687910009 1107 48-49 1.265476819140055 1107 50-51 1.2914808685755332 1107 52-53 1.3051547823630614 1107 54-55 1.212886034848914 1107 56-57 1.2031501840521557 1107 58-59 1.0558213636084943 1107 60-61 1.4852699790813872 1107 62-63 1.1995574729463954 1107 64-65 0.9855442996335597 1107 66-67 1.3512465329792107 1107 68-69 1.4846778602517858 1107 70-71 1.3228940787969492 1107 72-73 1.268074528386009 1107 74-75 1.4219502093491947 1107 76-77 1.4519094448690915 1107 78-79 1.2796777335327505 1107 80-81 1.211548521515077 1107 82-83 1.1592101328999007 1107 84-85 1.2345638783719544 1107 86-87 1.3534580516575936 1107 88-89 1.23908196452701 1107 90-91 1.2056327426794091 1107 92-93 1.3403830307700808 1107 94-95 1.5416383118282013 1107 96-97 1.8212741084820614 1107 98-99 2.18860559743748 1107 100-101 2.268466627662587 1108 1 0.19694952074246785 1108 2 0.11541160462798317 1108 3 0.12971845860059616 1108 4 0.19680394204223006 1108 5 0.21559421802737688 1108 6 0.22283942007429403 1108 7 0.1591757644317724 1108 8 0.2045489885472378 1108 9 0.11271537966896261 1108 10-11 0.29984305691861124 1108 12-13 0.20241502457569283 1108 14-15 0.30473178009007995 1108 16-17 0.31216294487096974 1108 18-19 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3 835.0 4 829.0 5 865.0 6 936.0 7 1106.0 8 1288.0 9 1589.0 10 1920.0 11 2475.0 12 3036.0 13 3814.0 14 4382.0 15 5190.0 16 6239.0 17 8271.0 18 7357.0 19 5584.0 20 5629.0 21 6134.0 22 6643.0 23 7391.0 24 8435.0 25 9602.0 26 10987.0 27 12848.0 28 15149.0 29 18114.0 30 22069.0 31 27102.0 32 34507.0 33 45057.0 34 60827.0 35 83985.0 36 118370.0 37 150841.0 38 105882.0 39 11838.0 40 2.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.31010157151844 18.485894201933707 14.48716460938025 22.716839617167604 2 15.03046021855161 19.638873319925708 41.14467901682303 24.185987444699652 3 20.098343280811946 23.619604184778893 29.928519795645254 26.353532738763906 4 12.511524029203569 15.886980869195705 37.73251064484499 33.86898445675573 5 13.56147656087822 37.083022968996126 34.683184154933876 14.672316315191775 6 31.917054054482858 35.53712149596584 17.542596387255195 15.003228062296111 7 28.344342736783528 32.3810697650012 20.94133755404533 18.33324994416994 8 26.033219720960197 34.603326853400986 19.68471651211235 19.678736913526464 9 25.427702476756757 17.449577606428292 21.28077639380115 35.841943523013796 10-11 25.155182472130623 26.04886996892689 27.381132545236635 21.41481501370585 12-13 26.05310818859045 23.545980883200063 28.026846997256477 22.37406393095301 14-15 22.66906036250823 25.096521798030082 26.808132300919784 25.426285538541897 16-17 21.915812324853064 28.4247346369482 27.34235479239845 22.31709824580029 18-19 21.56939196907126 27.56738814203009 29.305726405607786 21.557493483290866 20-21 22.563351666956464 26.024532279073743 29.283707479569145 22.12840857440065 22-23 21.994579363752514 25.97677009644144 29.656872017094166 22.371778522711878 24-25 22.5014613478081 25.9503031915348 29.50885289993642 22.03938256072068 26-27 21.96369200993579 25.893406828752784 30.14589459053293 21.997006570778492 28-29 21.55054738590508 26.9910281311383 29.68252776898063 21.77589671397599 30-31 21.840600332921657 26.102131772539334 29.56290608387478 22.49436181066423 32-33 21.71581474961175 26.256884036967087 29.773028915243582 22.25427229817758 34-35 22.63662557617606 26.101431394013503 29.377137968614043 21.884805061196396 36-37 22.11279275365679 25.90094240045491 29.922160138522987 22.064104707365317 38-39 21.87595703689785 26.572041772258466 29.736440426702714 21.815560764140965 40-41 21.984936425762545 26.58606161838864 29.505193431373865 21.923808524474953 42-43 21.575875901831882 26.563597260566496 29.035188602980433 22.825338234621192 44-45 21.999580232873132 26.263084165749444 29.037635051531296 22.699700549846128 46-47 22.471847042665832 26.727325864112906 28.785113786330996 22.015713306890266 48-49 22.266930522934565 26.640634963640437 28.775161386185133 22.317273127239865 50-51 21.630683766629204 26.982598603641367 29.289665701346202 22.09705192838323 52-53 22.13664943480259 27.300899348297452 28.22685522243873 22.33559599446123 54-55 21.794472856666946 26.724410390453496 28.632930762444126 22.848185990435432 56-57 22.185809217682323 26.97674929341351 28.36192113439388 22.47552035451029 58-59 22.78201772848454 26.714068705503397 28.387598601731362 22.116314964280704 60-61 22.180314908755516 26.83251470721001 29.253317814817255 21.73385256921722 62-63 21.831417231858648 27.013187609576484 29.1375530267815 22.01784213178337 64-65 21.70865527855964 27.505682462876408 28.56701890006064 22.21864335850332 66-67 21.867856953312724 27.147771354182872 28.08642144760866 22.897950244895743 68-69 21.620582897405786 26.958410224343822 28.651588403056323 22.76941847519407 70-71 22.285064491785935 26.870347199641774 28.313428310500054 22.53115999807223 72-73 22.151084052171154 26.49900562462635 28.91173850977904 22.43817181342346 74-75 21.517943974726762 27.72564040301492 28.247956433193455 22.508459189064858 76-77 21.91027515805284 26.954117548730544 28.147955638443182 22.98765165477343 78-79 22.10235467237378 26.990087388141472 28.27912958385089 22.62842835563386 80-81 22.29986157907227 26.99940153843057 28.134953388933525 22.56578349356364 82-83 22.102729544579798 26.910678156018765 28.681414466360582 22.305177833040855 84-85 21.996848949009213 27.266228431068168 28.183058617721883 22.553864002200736 86-87 21.865124168989137 27.310833899082542 28.402623124411758 22.421418807516567 88-89 21.81702280809843 27.103511800552667 27.906522786748244 23.17294260460066 90-91 21.97744948461281 26.785310138215397 28.081266284094035 23.15597409307776 92-93 22.40556527849878 26.832313898494913 28.289271015130176 22.472849807876134 94-95 22.015182126961818 26.654063695635198 28.44513742484579 22.885616752557194 96-97 21.375049336797534 26.818903603675597 29.02412882043724 22.78191823908963 98-99 21.759950119758944 27.097062702928266 28.0680679825712 23.07491919474159 100-101 21.862836179704136 26.272357334267042 28.65661098772127 23.20819549830755 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 899.0 1 529.5 2 178.0 3 198.0 4 164.5 5 159.0 6 264.0 7 491.5 8 776.5 9 960.5 10 951.5 11 861.5 12 864.0 13 945.0 14 1037.0 15 1101.5 16 1157.0 17 1228.5 18 1296.0 19 1364.0 20 1495.5 21 1744.5 22 2113.5 23 2598.0 24 3063.0 25 3728.5 26 4751.5 27 5968.5 28 6947.0 29 7953.0 30 9216.5 31 10526.0 32 12080.0 33 13620.0 34 15319.5 35 16699.0 36 18482.0 37 20664.0 38 22292.0 39 23272.0 40 24009.0 41 25322.5 42 26212.0 43 27112.5 44 28690.0 45 29928.5 46 30654.0 47 34135.5 48 40720.0 49 40381.5 50 34166.5 51 30437.5 52 28296.0 53 27189.0 54 26370.5 55 24401.0 56 22148.5 57 20034.5 58 17689.0 59 15225.5 60 12417.5 61 9858.5 62 7585.5 63 5539.0 64 4055.5 65 2941.5 66 2083.5 67 1598.5 68 1217.5 69 871.5 70 650.0 71 446.0 72 308.5 73 217.0 74 151.5 75 118.0 76 74.0 77 51.0 78 35.5 79 21.5 80 19.0 81 12.0 82 7.0 83 6.5 84 7.5 85 6.0 86 1.5 87 1.5 88 2.5 89 2.5 90 4.0 91 3.0 92 2.0 93 3.0 94 2.0 95 1.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.2828406359894611 2 0.24605430004251133 3 0.24227821920027479 4 0.24556706380480342 5 0.24690696345850024 6 0.1924583138946376 7 0.18344444349704064 8 0.1832008253781867 9 0.19233650483521061 10-11 0.19477268602375034 12-13 0.19160565047864872 14-15 0.19276283654320508 16-17 0.17863298564967472 18-19 0.18575881562615337 20-21 0.1735170051537413 22-23 0.18222635290277078 24-25 0.18368806161589463 26-27 0.1821654483730573 28-29 0.19032665535466534 30-31 0.1881949968146931 32-33 0.1923974093649241 34-35 0.18831680587412009 36-37 0.17711037240683739 38-39 0.16687841141497056 40-41 0.16614755705840867 42-43 0.17595318634228102 44-45 0.17723218146626438 46-47 0.1779021312931128 48-49 0.1914229368895082 50-51 0.18758595151755816 52-53 0.19994957104939723 54-55 0.1795465535953771 56-57 0.1641377075778634 58-59 0.16712202953382455 60-61 0.1572554957202387 62-63 0.16127519468132923 64-65 0.16200604903789115 66-67 0.16109248109218874 68-69 0.16377228039958244 70-71 0.16523398911270626 72-73 0.1640768030481499 74-75 0.17467419121829766 76-77 0.16730474312296503 78-79 0.16992363790064524 80-81 0.16511218005327927 82-83 0.1512259472786029 84-85 0.14988604762490604 86-87 0.1519568016351648 88-89 0.1573773047796657 90-91 0.16206695356760464 92-93 0.158778108963076 94-95 0.15944805878992444 96-97 0.16267599886473957 98-99 0.16980182884121822 100-101 0.15682916401224425 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 820957.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 70.09808014663787 #Duplication Level Percentage of deduplicated Percentage of total 1 86.42079951024984 60.579321304060166 2 8.465461056519894 11.868251352363467 3 2.1445517373331566 4.509868786865734 4 0.9516737777124415 2.668420189741615 5 0.4940126360522842 1.7314668677722431 6 0.33145699412747487 1.394069936370685 7 0.2181565679811005 1.0704649610798247 8 0.16589827019953166 0.9303320192508285 9 0.12233046382516344 0.7717617591832524 >10 0.6362637948403932 7.858058581473702 >50 0.034211065942600836 1.6365727851784164 >100 0.011693521762377137 1.4060800719485862 >500 0.0015707715541222723 0.7612137926250673 >1k 0.0019198318994827774 2.814117592086435 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 4141 0.5044113150871483 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 3237 0.3942959253651531 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2956 0.3600675796661701 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2567 0.31268385554907263 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 1963 0.23911118365517314 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 1939 0.2361877662289255 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1618 0.1970870581528631 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 1376 0.16760926577153248 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 1165 0.14190755423243848 No Hit NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 1032 0.12570694932864937 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 1008 0.12278353190240171 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 909 0.11072443501913011 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 1.2180905942698582E-4 0.0 8 0.0 0.0 0.0 1.2180905942698582E-4 0.0 9 0.0 0.0 0.0 1.2180905942698582E-4 0.0 10-11 0.0 0.0 0.0 2.4361811885397164E-4 0.0 12-13 0.0 0.0 0.0 2.4361811885397164E-4 0.0 14-15 0.0 0.0 0.0 2.4361811885397164E-4 0.0 16-17 0.0 0.0 0.0 3.6542717828095747E-4 0.0 18-19 0.0 0.0 0.0 4.872362377079433E-4 0.0 20-21 0.0 0.0 0.0 5.481407674214362E-4 0.0 22-23 0.0 0.0 0.0 7.917588862754079E-4 0.0 24-25 0.0 0.0 0.0 0.0011571860645563652 6.090452971349291E-5 26-27 0.0 0.0 0.0 0.0015835177725508159 1.2180905942698582E-4 28-29 0.0 0.0 0.0 0.0025579902479667023 1.2180905942698582E-4 30-31 0.0 0.0 0.0 0.005298694085073883 1.2180905942698582E-4 32-33 0.0 0.0 0.0 0.009318393046164416 2.4361811885397164E-4 34-35 0.0 0.0 0.0 0.013581710126108919 2.4361811885397164E-4 36-37 0.0 0.0 0.0 0.01833226344376137 2.4361811885397164E-4 38-39 0.0 0.0 0.0 0.022778294112846348 2.4361811885397164E-4 40-41 0.0 0.0 0.0 0.029234174262476598 2.4361811885397164E-4 42-43 0.0 0.0 0.0 0.04025789414061881 2.4361811885397164E-4 44-45 0.0 0.0 0.0 0.057067544341542854 2.4361811885397164E-4 46-47 0.0 0.0 0.0 0.09013870397596951 2.4361811885397164E-4 48-49 0.0 0.0 0.0 0.15427117376427757 2.4361811885397164E-4 50-51 0.0 0.0 0.0 0.1940418316671884 2.4361811885397164E-4 52-53 0.0 0.0 0.0 0.22778294112846348 3.0452264856746454E-4 54-55 0.0 0.0 0.0 0.27266957952730775 3.6542717828095747E-4 56-57 0.0 0.0 0.0 0.31164847854394323 3.6542717828095747E-4 58-59 0.0 0.0 0.0 0.3582404437747653 3.6542717828095747E-4 60-61 0.0 0.0 0.0 0.43205673378751874 3.6542717828095747E-4 62-63 0.0 0.0 0.0 0.49521473110041087 3.6542717828095747E-4 64-65 0.0 0.0 0.0 0.5573982559378872 3.6542717828095747E-4 66-67 0.0 0.0 0.0 0.6583170616731449 3.6542717828095747E-4 68-69 0.0 0.0 0.0 0.7701986827568313 4.872362377079433E-4 70-71 0.0 0.0 0.0 0.8783651275279948 4.872362377079433E-4 72-73 0.0 0.0 0.0 0.9951191109887607 6.090452971349291E-4 74-75 0.0 0.0 0.0 1.103894601057059 6.090452971349291E-4 76-77 0.0 0.0 0.0 1.2048743113220302 6.090452971349291E-4 78-79 0.0 0.0 0.0 1.3339919143146353 6.090452971349291E-4 80-81 0.0 0.0 0.0 1.4775438908493381 6.69949826848422E-4 82-83 0.0 0.0 0.0 1.633094059737599 7.308543565619149E-4 84-85 0.0 0.0 0.0 1.8004597073902775 7.308543565619149E-4 86-87 0.0 0.0 0.0 2.082508584493463 7.917588862754079E-4 88-89 0.0 0.0 0.0 2.4800933544631447 8.526634159889008E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTACATG 2825 0.0 27.095724 1 TACATGG 2855 0.0 26.140106 2 ACATGGG 2825 0.0 24.565279 3 GTATAGA 220 2.1427695E-9 23.771872 1 CGTATAT 50 0.0016559521 23.745054 90-91 GAGTACT 2765 0.0 22.851007 12-13 TCTAGAC 340 0.0 22.368063 3 CCTACAC 355 0.0 21.422934 3 CATGGGG 1545 0.0 21.227943 4 GTACTTT 2975 0.0 21.078308 14-15 GTCTAGG 300 1.6552804E-10 20.602287 1 AGTACTT 2945 0.0 20.24451 12-13 AGAGTAC 4055 0.0 20.209105 10-11 CTAGACT 435 0.0 19.668468 4 TAGACTC 295 2.9867806E-9 19.335106 5 TACAGCG 150 7.6834473E-4 19.012854 5 AGGATCG 125 0.0060061933 19.012852 5 GTACAAG 555 0.0 18.846167 1 ACTTTTT 3490 0.0 18.716562 16-17 GATCCCG 65 0.0075549367 18.273224 44-45 >>END_MODULE