FastQCFastQC Report
Thu 26 May 2016
SRR937686_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937686_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences820957
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT65710.8004073294947239No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT42880.5223172468229152No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT38640.4706702056258732No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT23860.29063641579278815No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT19840.24166917390313988No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT16000.1948944950831773No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC15210.18527157938844543No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT14240.17345610062402783No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC13390.16310233057273402No Hit
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT12990.15822996819565457No Hit
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA12780.1556719779476879No Hit
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG12190.14848524344149572No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC11930.14531820789639407No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10040.12229629566469376No Hit
GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA8680.10573026358262369No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTTCGC300.00395149131.66658678-79
GTATAGG2900.029.4934621
GTCTACG650.00583275729.2413791
CTCGCGC605.386177E-627.70657560-61
TACCTGG6900.026.8543012
TCGCGCT651.0029422E-525.575360-61
CGCGGAA751.0608801E-625.33249782-83
GGGCGTA1151.2818695E-424.7810369
GTACCTG7950.023.9080471
AACGCGA500.001653971223.74993976-77
ATTATAC3500.023.0769923
CCTCGCG753.0357221E-522.1652658-59
GTACATG28800.022.1087191
TACATGG28600.021.5961152
TACGCCC1100.00287265121.5961154
GCGTCAT550.002878945821.58953910-11
GTATACA2951.3278623E-1020.9398021
GAGTACT39400.020.79512-13
GGACCGA1404.8521897E-420.355856
ACATGGG29550.020.2586923
Produced by FastQC (version 0.11.5)