##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937686_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 820957 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.41953476247843 33.0 31.0 34.0 31.0 34.0 2 32.63950243435405 34.0 31.0 34.0 31.0 34.0 3 32.71851388075137 34.0 31.0 34.0 31.0 34.0 4 36.271773064849924 37.0 35.0 37.0 35.0 37.0 5 36.20891106355144 37.0 35.0 37.0 35.0 37.0 6 36.25926327444677 37.0 37.0 37.0 35.0 37.0 7 36.21597111663583 37.0 37.0 37.0 35.0 37.0 8 36.20153552500314 37.0 36.0 37.0 35.0 37.0 9 37.91951710016481 39.0 38.0 39.0 35.0 39.0 10-11 37.84801640037176 39.0 38.0 39.0 35.0 39.0 12-13 37.766039512422694 39.0 37.0 39.0 35.0 39.0 14-15 39.01963927465141 40.0 38.0 41.0 36.0 41.0 16-17 38.99548271103115 40.0 38.0 41.0 36.0 41.0 18-19 38.96756955601816 40.0 38.0 41.0 36.0 41.0 20-21 38.86213041608757 40.0 38.0 41.0 35.0 41.0 22-23 38.702545322105784 40.0 38.0 41.0 35.0 41.0 24-25 38.771425909030555 40.0 38.0 41.0 35.0 41.0 26-27 38.63517577656321 40.0 38.0 41.0 34.5 41.0 28-29 38.50629021982881 40.0 38.0 41.0 34.0 41.0 30-31 38.5857529687913 40.0 38.0 41.0 35.0 41.0 32-33 38.51974342139722 40.0 38.0 41.0 35.0 41.0 34-35 38.38244390388291 40.0 38.0 41.0 34.0 41.0 36-37 38.224725533736844 40.0 38.0 41.0 34.0 41.0 38-39 38.08784747556815 40.0 38.0 41.0 34.0 41.0 40-41 37.938499214940606 40.0 37.5 41.0 33.5 41.0 42-43 37.72263955359416 40.0 37.0 41.0 33.0 41.0 44-45 37.41610096509318 40.0 37.0 41.0 33.0 41.0 46-47 37.0586072108527 40.0 37.0 41.0 32.0 41.0 48-49 36.69945928958521 40.0 36.5 41.0 31.0 41.0 50-51 36.4248974063197 39.0 36.0 41.0 30.5 41.0 52-53 36.210475091874486 39.0 36.0 41.0 30.0 41.0 54-55 35.99179859602879 39.0 35.0 40.0 29.5 41.0 56-57 35.69859992667095 39.0 35.0 40.0 28.5 41.0 58-59 35.4192418116905 38.0 35.0 40.0 28.0 41.0 60-61 35.0045495683696 38.0 34.0 40.0 27.5 41.0 62-63 34.67211681001563 37.5 34.0 40.0 27.0 41.0 64-65 34.27804075000274 37.0 34.0 39.5 26.0 41.0 66-67 33.82788988948264 36.5 33.5 39.0 26.0 40.5 68-69 33.3619964261222 36.0 33.0 39.0 25.0 40.0 70-71 32.89949960838388 35.0 32.5 38.5 24.5 40.0 72-73 32.37019843913871 35.0 32.0 37.5 22.5 39.5 74-75 31.869009582718704 35.0 31.0 37.0 21.5 39.0 76-77 30.674593797239076 33.5 30.0 35.5 20.5 38.0 78-79 31.270626232555422 34.0 31.0 36.0 20.5 38.5 80-81 31.049180407743645 34.0 31.0 36.0 20.0 37.0 82-83 30.671738592886108 34.0 31.0 35.0 18.5 37.0 84-85 30.278012124873776 34.0 30.5 35.0 17.0 36.5 86-87 29.873696186280156 34.0 30.0 35.0 10.0 36.0 88-89 29.495243965274675 34.0 30.0 35.0 7.0 36.0 90-91 28.952294699966014 34.0 29.0 35.0 2.0 35.0 92-93 28.46300537056143 34.0 29.0 35.0 2.0 35.0 94-95 27.95298657542356 33.0 27.5 35.0 2.0 35.0 96-97 27.357208721041417 33.0 27.0 35.0 2.0 35.0 98-99 26.45823289161308 33.0 25.0 34.5 2.0 35.0 100-101 23.759048281456884 30.0 12.5 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.1491089728014714 1101 2 0.11411363287486154 1101 3 0.05921137587233005 1101 4 0.013169543291617458 1101 5 0.017652828389707054 1101 6 -0.01900429757082378 1101 7 0.045283520322492166 1101 8 0.08276681099798111 1101 9 -0.02636271988811245 1101 10-11 0.046709332801690095 1101 12-13 0.018202862029269795 1101 14-15 0.03983722663756595 1101 16-17 0.0950085163581349 1101 18-19 0.030242537028115635 1101 20-21 0.003343076129887379 1101 22-23 0.04035599593464667 1101 24-25 0.05759833389358704 1101 26-27 -0.10402021485820967 1101 28-29 0.02986110803718134 1101 30-31 0.04030161339570526 1101 32-33 0.14855150172378018 1101 34-35 -0.11730318462820577 1101 36-37 -0.11685535124265556 1101 38-39 -0.307123610072523 1101 40-41 -0.4637005610169993 1101 42-43 -0.30153263022635457 1101 44-45 -0.27213136282419725 1101 46-47 -0.4552136519772958 1101 48-49 -0.44457555529159976 1101 50-51 -0.34671231442232653 1101 52-53 -0.4115808001077994 1101 54-55 -0.3888339993519807 1101 56-57 -0.37062331190891484 1101 58-59 -0.34845082160070007 1101 60-61 -0.43193736519499026 1101 62-63 -0.38606358435597343 1101 64-65 -0.4624009618890881 1101 66-67 -0.7419927726816979 1101 68-69 -0.5572608491067825 1101 70-71 -0.4119583144789374 1101 72-73 -0.3856459331936932 1101 74-75 -0.27955738889968273 1101 76-77 -0.4934264165893758 1101 78-79 -0.3606745308606598 1101 80-81 -0.314653427500847 1101 82-83 -0.3388923784627522 1101 84-85 -0.23378698776775764 1101 86-87 -0.4381597060553304 1101 88-89 -0.383824292378403 1101 90-91 -0.4723395239334103 1101 92-93 -0.06474826180475901 1101 94-95 -0.13172926793644635 1101 96-97 0.1934397385487081 1101 98-99 0.24632448536286233 1101 100-101 0.6462968468994923 1102 1 0.036357709983754205 1102 2 0.10427272263525822 1102 3 0.03842356789792234 1102 4 0.0035189104705466434 1102 5 -0.09521587127225217 1102 6 0.013332457782958329 1102 7 0.0875726765907956 1102 8 -0.05843835860163438 1102 9 0.04331449230986095 1102 10-11 0.04624627486519017 1102 12-13 -9.943456496159797E-4 1102 14-15 0.22038225251827015 1102 16-17 0.18144946643707272 1102 18-19 -0.1270975630459077 1102 20-21 0.06244428290494852 1102 22-23 -0.04617934958238834 1102 24-25 0.045964653601267 1102 26-27 0.08334952686993091 1102 28-29 0.1304078583069952 1102 30-31 0.042975159780283434 1102 32-33 -0.0502720486744721 1102 34-35 -0.0814559595567772 1102 36-37 -0.026778318619001595 1102 38-39 -0.09718146630009272 1102 40-41 -0.08845565316775605 1102 42-43 -0.08831266061127963 1102 44-45 -0.013797806509515453 1102 46-47 0.06502339206301144 1102 48-49 -0.013171307942847932 1102 50-51 -0.06888349869114307 1102 52-53 0.0077653591127813115 1102 54-55 0.006009482605854544 1102 56-57 -0.11253057545206246 1102 58-59 0.07206970606236496 1102 60-61 -0.12715084753933326 1102 62-63 0.037457529004775836 1102 64-65 0.2039951540731053 1102 66-67 0.295051862238374 1102 68-69 0.20366746684887715 1102 70-71 -0.0656214520941063 1102 72-73 -0.07391280321934346 1102 74-75 -0.06270505309571206 1102 76-77 -0.020368509819363112 1102 78-79 0.018619718301675192 1102 80-81 0.09325304520177724 1102 82-83 0.001308780445651081 1102 84-85 -0.1284833020346987 1102 86-87 -0.038721321748461435 1102 88-89 0.15720130290747392 1102 90-91 0.21215078529712272 1102 92-93 0.21541293884574841 1102 94-95 0.1611336936721095 1102 96-97 0.08051232046462431 1102 98-99 0.12427267044332879 1102 100-101 0.6949090109780869 1103 1 0.14947147231217883 1103 2 0.1817231953736922 1103 3 0.17675992612957003 1103 4 0.09896534884892816 1103 5 -0.0024029250561667936 1103 6 0.06733866100382357 1103 7 0.022240975133442475 1103 8 0.12308169202836439 1103 9 0.10186850374345369 1103 10-11 0.10574654771625092 1103 12-13 0.2981728919993003 1103 14-15 0.25203559045433366 1103 16-17 0.21045057004550216 1103 18-19 0.15701099854955913 1103 20-21 0.09178274548948195 1103 22-23 0.09573545463607758 1103 24-25 0.2612707262371856 1103 26-27 0.21946166952658075 1103 28-29 0.12266821575059339 1103 30-31 0.2749081482577793 1103 32-33 0.26238911215423144 1103 34-35 0.19986176286042223 1103 36-37 0.13048631901212815 1103 38-39 -0.049231161805280976 1103 40-41 -0.15135915455293514 1103 42-43 0.00651877322538752 1103 44-45 -0.13219293668475274 1103 46-47 0.08452003852300294 1103 48-49 0.031157399998427593 1103 50-51 0.1934898173470998 1103 52-53 0.03956558332138371 1103 54-55 -0.11040246495792161 1103 56-57 -0.05299630518479859 1103 58-59 0.1255594100756312 1103 60-61 -0.013182355298589243 1103 62-63 -0.05371918259078967 1103 64-65 0.19627468954635674 1103 66-67 0.09397109309477258 1103 68-69 0.0060731763226513635 1103 70-71 0.003253390340951512 1103 72-73 0.02202093145784545 1103 74-75 -0.04662209591327837 1103 76-77 0.01387037520486345 1103 78-79 0.3213976558963658 1103 80-81 0.2189637219595717 1103 82-83 0.34305429496603423 1103 84-85 0.20678308951441338 1103 86-87 0.17168955881948733 1103 88-89 0.0332105467177648 1103 90-91 -0.0055320554223676766 1103 92-93 0.18119148839393517 1103 94-95 0.12019470247133768 1103 96-97 0.30153649698618423 1103 98-99 0.4454022389550971 1103 100-101 0.814387699828508 1104 1 0.18428386630359483 1104 2 0.10338420255351366 1104 3 0.02844985535655553 1104 4 0.020715206744732484 1104 5 0.021024047059519546 1104 6 0.057685828089873326 1104 7 -0.003111095828664645 1104 8 -3.0347468634062125E-4 1104 9 0.10018375747489472 1104 10-11 0.15930473411366108 1104 12-13 0.16558028506233313 1104 14-15 0.19774837254112043 1104 16-17 0.04741178442974814 1104 18-19 0.143795593657984 1104 20-21 0.08818201326845809 1104 22-23 0.13105304468571433 1104 24-25 0.11955212556761552 1104 26-27 0.15549029312646212 1104 28-29 -0.006167031100616782 1104 30-31 0.23151653417060913 1104 32-33 0.24307091609715314 1104 34-35 0.20561313805307435 1104 36-37 0.11464810933127723 1104 38-39 0.28197184271584774 1104 40-41 0.2694851095068387 1104 42-43 0.2429157855631061 1104 44-45 0.23635641590109202 1104 46-47 -0.022104807554519823 1104 48-49 -0.07529259242648578 1104 50-51 0.12141650867173581 1104 52-53 0.26025082738176764 1104 54-55 0.29738188296325063 1104 56-57 0.14039069549980354 1104 58-59 0.13400592365326958 1104 60-61 0.08599099130919541 1104 62-63 0.2360651493828243 1104 64-65 0.2268364880671072 1104 66-67 0.16770693535969627 1104 68-69 -0.01506816807981437 1104 70-71 0.0453395823701257 1104 72-73 0.08424377133176364 1104 74-75 0.06871980278095435 1104 76-77 0.20057648551416563 1104 78-79 0.37089206114903917 1104 80-81 0.39528967650673863 1104 82-83 0.34466955541823907 1104 84-85 0.35296871688590414 1104 86-87 0.42226737325135133 1104 88-89 0.13598975154942394 1104 90-91 0.1973334940794409 1104 92-93 0.1516235648693396 1104 94-95 1.2947693291209816E-4 1104 96-97 0.4277734299505944 1104 98-99 0.6091563867457346 1104 100-101 0.7870051621837106 1105 1 0.13899480245247986 1105 2 0.13312012749041457 1105 3 0.07767263809626712 1105 4 -0.08817142183540483 1105 5 0.106267325002257 1105 6 0.08340742514456423 1105 7 0.08308629947490687 1105 8 -0.021950653518125307 1105 9 0.06529325380058992 1105 10-11 0.019067802297144 1105 12-13 0.06190466151512908 1105 14-15 -0.040945849699348 1105 16-17 0.05389054908959423 1105 18-19 0.23197523739554526 1105 20-21 0.19298103306309855 1105 22-23 0.08712773844481347 1105 24-25 0.1976299967866879 1105 26-27 0.0805825537837137 1105 28-29 0.05004674981293533 1105 30-31 0.11197339663407746 1105 32-33 0.11452155554631815 1105 34-35 0.11011346488589879 1105 36-37 0.1869639031246777 1105 38-39 0.06907469686046852 1105 40-41 0.06779701061865495 1105 42-43 0.21171717379653643 1105 44-45 0.4379265325349735 1105 46-47 0.41982740467869917 1105 48-49 0.27283859797782384 1105 50-51 0.4725661394825451 1105 52-53 0.4250881584326436 1105 54-55 0.33813485890073736 1105 56-57 0.2738227626368328 1105 58-59 0.19116282979215526 1105 60-61 0.050664466260315066 1105 62-63 0.29556777723602323 1105 64-65 0.5281067392447341 1105 66-67 0.13385408927597098 1105 68-69 -0.04333114686060213 1105 70-71 0.10398042680062503 1105 72-73 0.014899518166757275 1105 74-75 0.021537893197866254 1105 76-77 -0.2007690984320405 1105 78-79 -0.05761728802860233 1105 80-81 -0.18141417777565394 1105 82-83 -0.10134513593725458 1105 84-85 -0.17472999893954722 1105 86-87 -9.796742610603815E-4 1105 88-89 -0.07083900270453469 1105 90-91 -0.13977185161589034 1105 92-93 -0.10672239720632604 1105 94-95 -0.09978390503940204 1105 96-97 -0.24056086580526426 1105 98-99 -0.40071308530932725 1105 100-101 -0.1737096783717007 1106 1 0.07798160815659116 1106 2 0.08156665233985905 1106 3 0.119980316458566 1106 4 0.0036629079893586436 1106 5 -0.0022747034892987017 1106 6 0.017109623468719803 1106 7 -0.009246578215886814 1106 8 -0.09463711948452413 1106 9 -0.0361204466573497 1106 10-11 0.04679703460594453 1106 12-13 -0.13800306180712596 1106 14-15 0.12857583441347487 1106 16-17 0.019727329956964468 1106 18-19 0.20544070706389306 1106 20-21 0.14267120822561452 1106 22-23 0.02910673779579298 1106 24-25 0.1610402092224632 1106 26-27 0.09900525710965269 1106 28-29 0.22205120229860142 1106 30-31 0.19767649415729238 1106 32-33 0.15952864399051947 1106 34-35 0.029640587702715493 1106 36-37 -0.11206238757042541 1106 38-39 -0.14323236511538084 1106 40-41 0.03797478372317187 1106 42-43 -0.05318034289121698 1106 44-45 -0.16529734990199785 1106 46-47 -0.059605229720745 1106 48-49 0.1170559866022316 1106 50-51 0.12423255838602643 1106 52-53 -9.735672318740285E-4 1106 54-55 0.2694947887052024 1106 56-57 0.04588577183549347 1106 58-59 0.2250523008238119 1106 60-61 -0.0018453143423684537 1106 62-63 -0.00984627129897575 1106 64-65 -0.046179612146310944 1106 66-67 0.0542350886196985 1106 68-69 -0.009805162545823976 1106 70-71 0.2649986972202427 1106 72-73 0.1856802622539817 1106 74-75 0.21482308795785343 1106 76-77 0.15450970766366012 1106 78-79 0.16769099584274016 1106 80-81 -0.01157161012723762 1106 82-83 -0.20460890220351047 1106 84-85 -0.045277966495866906 1106 86-87 0.004905003459473534 1106 88-89 -0.09754938446529593 1106 90-91 0.010535797739986918 1106 92-93 0.34589561360581556 1106 94-95 0.42097290875025095 1106 96-97 0.3690698099355778 1106 98-99 0.24346877044899173 1106 100-101 0.49137221038142087 1107 1 0.3496526952637993 1107 2 0.46063972476460435 1107 3 0.48660567810055966 1107 4 0.18275632068543501 1107 5 0.18200280204406027 1107 6 0.227639460773311 1107 7 0.30970493432415225 1107 8 0.2533233668620696 1107 9 0.2660610474426335 1107 10-11 0.33013032333342096 1107 12-13 0.3127948923898032 1107 14-15 0.4189559566156831 1107 16-17 0.47266368899486366 1107 18-19 0.42339072887615714 1107 20-21 0.2818602201969398 1107 22-23 0.3708540741569806 1107 24-25 0.4820404688285649 1107 26-27 0.45131615280421045 1107 28-29 0.5027937827831863 1107 30-31 0.5141992177196215 1107 32-33 0.5854512492581136 1107 34-35 0.5315755898304175 1107 36-37 0.688423637860268 1107 38-39 0.4932709281351322 1107 40-41 0.49528831562394515 1107 42-43 0.5487658999127447 1107 44-45 0.6706048348037257 1107 46-47 0.49257024258838555 1107 48-49 0.5971265352275239 1107 50-51 0.6209286024708902 1107 52-53 0.4676033899170662 1107 54-55 0.7372460914192871 1107 56-57 0.6191067340909129 1107 58-59 0.7465607995779706 1107 60-61 0.906848278204528 1107 62-63 0.7017862228197416 1107 64-65 0.7398487625321906 1107 66-67 0.7484646071708241 1107 68-69 0.7231966600495454 1107 70-71 0.813043221003646 1107 72-73 0.7252086355943135 1107 74-75 0.79415951336928 1107 76-77 0.8058051956026837 1107 78-79 1.0320655458811423 1107 80-81 0.6077950045289988 1107 82-83 0.7044955102971926 1107 84-85 0.8877690864396897 1107 86-87 0.9509283465971734 1107 88-89 0.8402147379724312 1107 90-91 0.8685085150128913 1107 92-93 1.0179221201909812 1107 94-95 1.5307983303425203 1107 96-97 1.5313436916890133 1107 98-99 1.9542207449273015 1107 100-101 2.1254627727631643 1108 1 0.14419994384065404 1108 2 0.047059459109952684 1108 3 -0.06300191203227001 1108 4 0.026099015182595053 1108 5 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-0.21980770920097115 2316 48-49 -0.0935857813485299 2316 50-51 -0.09453348011015805 2316 52-53 0.014128755997759868 2316 54-55 0.09970817911498386 2316 56-57 0.11486413626054315 2316 58-59 0.056147689720511096 2316 60-61 -0.06443299145488623 2316 62-63 0.3306982867323427 2316 64-65 0.3471898121595913 2316 66-67 0.30920868569085513 2316 68-69 0.3455261761530082 2316 70-71 0.5696398340858835 2316 72-73 0.4724761366300285 2316 74-75 0.5700536811398536 2316 76-77 0.6874420865124229 2316 78-79 0.3653536998524096 2316 80-81 0.23863273219462044 2316 82-83 0.18729495996141665 2316 84-85 0.16989544885318963 2316 86-87 0.4580070149482651 2316 88-89 0.7059246250922229 2316 90-91 0.525927051508436 2316 92-93 0.8359046997109161 2316 94-95 0.9906355823461794 2316 96-97 0.7247642126667309 2316 98-99 0.8606662202568138 2316 100-101 1.4696758079468033 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 2.0 3 3.0 4 5.0 5 11.0 6 29.0 7 62.0 8 144.0 9 285.0 10 475.0 11 871.0 12 1387.0 13 2247.0 14 2790.0 15 3853.0 16 5458.0 17 8253.0 18 7683.0 19 4744.0 20 3869.0 21 3893.0 22 3923.0 23 4238.0 24 4896.0 25 5560.0 26 6729.0 27 8462.0 28 10480.0 29 13090.0 30 16963.0 31 21804.0 32 28261.0 33 38795.0 34 55589.0 35 81922.0 36 129474.0 37 191530.0 38 140794.0 39 12382.0 40 1.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.092989708557354 19.19810326531259 14.933496105529015 21.775410920601036 2 15.743942739997344 20.69742995065515 39.87894615674146 23.679681152606044 3 20.94847842213417 24.036947123905396 29.831038653668827 25.18353580029161 4 13.130910791808576 16.744015513620706 37.05630996058254 33.068763733988185 5 13.575823292194105 37.68503514906371 34.17049427990107 14.568647278841116 6 32.16282947827962 36.57097752988281 16.674198526841234 14.591994464996338 7 28.791641949578356 33.08407139472591 20.11359912882161 18.010687526874126 8 26.26264225775528 35.08052187873421 18.814505510032802 19.84233035347771 9 25.813654064707407 17.980844307314513 20.314584076876134 35.89091755110195 10-11 25.52752458411342 26.684893362258926 26.607849132171356 21.179732921456303 12-13 26.843866406476497 23.957046695365545 27.32118625279863 21.877900645359333 14-15 22.847298945011737 25.674791736960646 26.622222601183744 24.855686716843877 16-17 21.990798543650886 28.79937682485197 27.13290708283138 22.076917548665765 18-19 21.64370362881369 27.86546676622527 29.437108155481955 21.053721449479085 20-21 22.65118635933429 25.91097950318957 29.815995234829597 21.621838902646545 22-23 22.065811479613277 26.14480035958297 29.802570433558156 21.986817727245597 24-25 22.720996788502195 26.14346801566222 29.88721083272987 21.24832436310572 26-27 22.201210057092187 26.017323768839383 30.42305710558339 21.35840906848504 28-29 21.536843336187754 27.316922244865395 30.043218275434892 21.10301614351196 30-31 22.000056032167336 26.28444607939271 29.701981955205937 22.013515933234018 32-33 21.991468493477733 26.567530333501022 29.831160462728256 21.60984071029299 34-35 22.71416164305804 26.21818195106443 29.82476548710834 21.24289091876919 36-37 22.433269952019412 25.98522212491032 30.129714467383796 21.451793455686474 38-39 21.897005567892105 26.75889236586082 30.23282583618874 21.111276230058333 40-41 22.0817685830214 26.73429961106083 29.68587650176442 21.498055304153354 42-43 21.701885298327902 26.92126823622575 29.207870130661224 22.168976334785125 44-45 22.445329048902682 26.34041734219941 29.055784894945774 22.158468713952132 46-47 22.967950818374167 27.125659443795474 28.635543639922677 21.270846097907686 48-49 22.795103763047273 27.1357086911982 28.555271469760292 21.513916075994235 50-51 21.919966575594092 27.470135463854987 29.105482991191987 21.504414969358933 52-53 22.645887665249216 27.957615319681782 27.769968463634516 21.626528551434486 54-55 22.15371816063448 27.454543904248336 28.074064780493984 22.3176731546232 56-57 22.66616887364381 27.511002403292746 28.15330157365124 21.66952714941221 58-59 23.27582321607587 27.155380854295657 27.9039584290042 21.66483750062427 60-61 22.440456686525604 27.450767823406096 29.001214436322485 21.107561053745812 62-63 22.14123273204321 27.47263254957324 28.883181457737738 21.502953260645807 64-65 21.917408585346127 28.22693515007485 28.28241917664384 21.57323708793518 66-67 22.26864500820384 27.539140296020374 27.88392083872846 22.308293857047325 68-69 22.006768933554945 27.58483549371983 28.17226004057462 22.23613553215061 70-71 22.81179160418877 27.527812053493665 27.895736317492876 21.764660024824686 72-73 22.62359660737408 27.116523764338453 28.49716854841362 21.762711079873853 74-75 21.925935219506016 28.537365538024524 27.656137897599997 21.880561344869463 76-77 22.287586316944736 27.641094478760763 27.777155198140708 22.294164006153792 78-79 22.54052282884487 27.759005648286085 27.671059507379802 22.02941201548924 80-81 22.82934601285208 27.70798452780385 27.65030790303749 21.812361556306577 82-83 22.64674157659224 27.603854842275606 27.980069225395475 21.769334355736675 84-85 22.271974819447202 28.009223449389793 27.881323002225468 21.837478728937537 86-87 22.231615054138036 28.116576142234003 27.630314377001476 22.021494426626486 88-89 22.07545583995264 28.221392837870923 27.118472709289282 22.584678612887153 90-91 22.47781724221738 27.50223396071031 27.40800254370005 22.61194625337226 92-93 22.79744463196695 27.62027849832844 27.667480572539844 21.914796297164767 94-95 22.607213288881148 27.582017084938677 27.688904534585856 22.121865091594323 96-97 21.826539027013595 27.7315377053853 28.390098385177296 22.051824882423805 98-99 22.255375234030218 28.153423936677548 27.143728964688087 22.447471864604154 100-101 21.79473512037657 28.050746943298343 27.336425113474583 22.8180928228505 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 207.0 1 154.0 2 140.5 3 203.0 4 180.5 5 153.5 6 263.5 7 606.0 8 1023.0 9 1276.0 10 1252.5 11 1144.0 12 1153.0 13 1241.5 14 1369.0 15 1471.0 16 1570.0 17 1623.0 18 1610.5 19 1666.0 20 1845.0 21 2053.0 22 2336.5 23 2724.5 24 3257.5 25 3947.5 26 4848.0 27 5981.5 28 6994.5 29 8167.0 30 9386.0 31 10444.5 32 11800.5 33 13376.5 34 15003.0 35 16444.5 36 18374.5 37 20256.0 38 21901.0 39 23216.0 40 24171.0 41 25257.5 42 25958.0 43 27012.5 44 28329.5 45 29241.0 46 29975.0 47 34101.5 48 41768.0 49 40537.5 50 33431.5 51 30134.5 52 28347.5 53 27331.5 54 26206.5 55 24366.5 56 21794.5 57 19610.5 58 17582.0 59 15395.0 60 12623.0 61 9856.5 62 7636.5 63 5504.5 64 3860.5 65 2771.0 66 2042.5 67 1602.5 68 1191.0 69 834.0 70 627.5 71 411.0 72 277.5 73 200.5 74 142.0 75 89.0 76 56.0 77 38.5 78 21.0 79 16.0 80 14.5 81 11.0 82 5.5 83 3.0 84 3.0 85 3.5 86 2.0 87 0.5 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.02168201257800348 2 0.0 3 0.0 4 1.2180905942698582E-4 5 0.020342112924306634 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0023143721291127304 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 7.308543565619149E-4 24-25 6.090452971349291E-5 26-27 4.872362377079433E-4 28-29 9.744724754158866E-4 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 7.308543565619149E-4 42-43 0.01607879584436213 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 6.090452971349291E-5 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 6.090452971349291E-5 82-83 0.0018880404211182803 84-85 7.308543565619149E-4 86-87 0.0 88-89 0.0 90-91 0.012850855769547004 92-93 0.002253467599399238 94-95 0.0 96-97 0.0 98-99 0.001948944950831773 100-101 0.02174291710771697 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 820957.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 66.83150057487539 #Duplication Level Percentage of deduplicated Percentage of total 1 85.15758648756534 56.91209290298723 2 9.092003976900285 12.152645380179614 3 2.3934179347366022 4.798671362437989 4 1.0372660759244055 2.772881933977626 5 0.5683766966297351 1.8992733763777958 6 0.38560463476288864 1.5462321821898362 7 0.2508682653195632 1.1736131832545666 8 0.1826983688593528 0.9768004914762106 9 0.13754957972531465 0.8273380334837611 >10 0.7331382519394279 8.811346662009882 >50 0.0396480316143982 1.8171324000137452 >100 0.018721453757409866 2.249430487601699 >500 5.506309879865062E-4 0.2636070505455443 >1k 0.0023860676146081937 2.9929029438160697 >5k 1.8354366266216872E-4 0.8060316096485673 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 6571 0.8004073294947239 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4288 0.5223172468229152 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3864 0.4706702056258732 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2386 0.29063641579278815 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 1984 0.24166917390313988 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 1600 0.1948944950831773 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 1521 0.18527157938844543 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 1424 0.17345610062402783 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 1339 0.16310233057273402 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 1299 0.15822996819565457 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 1278 0.1556719779476879 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 1219 0.14848524344149572 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 1193 0.14531820789639407 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1004 0.12229629566469376 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 868 0.10573026358262369 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 6.090452971349291E-5 0.0 12-13 0.0 0.0 0.0 1.2180905942698582E-4 0.0 14-15 0.0 0.0 0.0 1.2180905942698582E-4 1.8271358914047874E-4 16-17 0.0 0.0 0.0 2.4361811885397164E-4 3.6542717828095747E-4 18-19 0.0 0.0 0.0 2.4361811885397164E-4 3.6542717828095747E-4 20-21 0.0 0.0 0.0 3.0452264856746454E-4 3.6542717828095747E-4 22-23 0.0 0.0 0.0 4.872362377079433E-4 4.2633170799445035E-4 24-25 0.0 0.0 0.0 7.917588862754079E-4 4.872362377079433E-4 26-27 0.0 0.0 0.0 0.0012180905942698582 4.872362377079433E-4 28-29 0.0 0.0 0.0 0.0023143721291127304 4.872362377079433E-4 30-31 0.0 0.0 0.0 0.005176885025646897 4.872362377079433E-4 32-33 0.0 0.0 0.0 0.009135679457023937 4.872362377079433E-4 34-35 0.0 0.0 0.0 0.01339899653696844 4.872362377079433E-4 36-37 0.0 0.0 0.0 0.018332263443761366 4.872362377079433E-4 38-39 0.0 0.0 0.0 0.022717389583132856 4.872362377079433E-4 40-41 0.0 0.0 0.0 0.028990556143622625 4.872362377079433E-4 42-43 0.0 0.0 0.0 0.03977065790291087 4.872362377079433E-4 44-45 0.0 0.0 0.0 0.05664121263354841 4.872362377079433E-4 46-47 0.0 0.0 0.0 0.090199608505683 6.69949826848422E-4 48-49 0.0 0.0 0.0 0.15518474170997992 7.308543565619149E-4 50-51 0.0 0.0 0.0 0.1948944950831773 7.308543565619149E-4 52-53 0.0 0.0 0.0 0.22881831813359288 7.308543565619149E-4 54-55 0.0 0.0 0.0 0.27382676559186414 7.308543565619149E-4 56-57 0.0 0.0 0.0 0.3134147099056345 7.308543565619149E-4 58-59 0.0 0.0 0.0 0.36085933855244545 7.308543565619149E-4 60-61 0.0 0.0 0.0 0.4358328146297553 7.308543565619149E-4 62-63 0.0 0.0 0.0 0.49923443006150137 7.308543565619149E-4 64-65 0.0 0.0 0.0 0.5620270001961125 7.308543565619149E-4 66-67 0.0 0.0 0.0 0.6638593738770727 7.308543565619149E-4 68-69 0.0 0.0 0.0 0.776471849317321 7.308543565619149E-4 70-71 0.0 0.0 0.0 0.8853691484450464 7.308543565619149E-4 72-73 0.0 0.0 0.0 1.0030366998515148 8.526634159889008E-4 74-75 0.0 0.0 0.0 1.112177617098094 8.526634159889008E-4 76-77 0.0 0.0 0.0 1.2142536088979081 8.526634159889008E-4 78-79 0.0 0.0 0.0 1.344954729663064 8.526634159889008E-4 80-81 0.0 0.0 0.0 1.4897247967920366 8.526634159889008E-4 82-83 0.0 0.0 0.0 1.6468584834528484 8.526634159889008E-4 84-85 0.0 0.0 0.0 1.8150158899918023 8.526634159889008E-4 86-87 0.0 0.0 0.0 2.1012671796452187 8.526634159889008E-4 88-89 0.0 0.0 0.0 2.504211548229688 8.526634159889008E-4 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTTTCGC 30 0.003951491 31.666586 78-79 GTATAGG 290 0.0 29.493462 1 GTCTACG 65 0.005832757 29.241379 1 CTCGCGC 60 5.386177E-6 27.706575 60-61 TACCTGG 690 0.0 26.854301 2 TCGCGCT 65 1.0029422E-5 25.5753 60-61 CGCGGAA 75 1.0608801E-6 25.332497 82-83 GGGCGTA 115 1.2818695E-4 24.781036 9 GTACCTG 795 0.0 23.908047 1 AACGCGA 50 0.0016539712 23.749939 76-77 ATTATAC 350 0.0 23.076992 3 CCTCGCG 75 3.0357221E-5 22.16526 58-59 GTACATG 2880 0.0 22.108719 1 TACATGG 2860 0.0 21.596115 2 TACGCCC 110 0.002872651 21.596115 4 GCGTCAT 55 0.0028789458 21.589539 10-11 GTATACA 295 1.3278623E-10 20.939802 1 GAGTACT 3940 0.0 20.795 12-13 GGACCGA 140 4.8521897E-4 20.35585 6 ACATGGG 2955 0.0 20.258692 3 >>END_MODULE