FastQCFastQC Report
Thu 26 May 2016
SRR937685_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937685_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences738838
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT36030.4876576461957831No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT30330.4105094756902054No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT28800.3898012825544977No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT24680.33403804352239597No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC19090.25837869735990837No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT18700.25310013832531625No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT15150.20505171634377226No Hit
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT12770.17283897146600471No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT10360.14022018358557628No Hit
ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC9290.12573798315733625No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN9230.1249258971520144No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC8550.1157222557583665No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC7510.10164609833278743No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGACCGT953.4881457E-530.0012866
TATACCG650.005829905629.243925
TAGCGTG650.005841510429.2320219
GACCGTC856.4033805E-427.9423737
GTACATG23450.026.9579031
TACCTGG6000.026.1367512
GTACCTG6200.026.0654091
TACATGG23950.025.7944772
ACCGTCC1151.280772E-424.783678
TGGACCG1351.33721005E-524.640715
ACATGGG24000.023.9554393
GAGTACT25800.022.82888812-13
GTACTAG1503.0160583E-522.181151
GTAAGCG753.0369341E-522.16385894-95
TACCGCA1604.9845323E-520.7821397
GTACTTT28200.020.54773914-15
GTGTAGC3252.1827873E-1120.474911
GTCTAGA3503.6379788E-1220.3704451
AGAGTAC37300.019.73811310-11
GAGTACA18350.019.1678311
Produced by FastQC (version 0.11.5)