##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937685_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 738838 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.83353048976907 33.0 31.0 34.0 30.0 34.0 2 31.978318657134583 33.0 31.0 34.0 30.0 34.0 3 32.00250122489639 34.0 31.0 34.0 30.0 34.0 4 35.5638245461116 37.0 35.0 37.0 33.0 37.0 5 35.54956296238147 37.0 35.0 37.0 33.0 37.0 6 35.606614711208685 37.0 35.0 37.0 33.0 37.0 7 35.550457610464 37.0 35.0 37.0 33.0 37.0 8 35.53599029827919 37.0 35.0 37.0 33.0 37.0 9 37.16942821024365 39.0 37.0 39.0 34.0 39.0 10-11 37.0568006789039 39.0 37.0 39.0 33.0 39.0 12-13 36.932405344608696 39.0 37.0 39.0 33.0 39.0 14-15 38.10562802671222 40.0 38.0 41.0 33.0 41.0 16-17 38.03492917256557 40.0 38.0 41.0 33.0 41.0 18-19 37.96372344140393 40.0 38.0 41.0 33.0 41.0 20-21 37.86848876208316 40.0 38.0 41.0 33.0 41.0 22-23 37.669259025659215 40.0 37.5 41.0 32.5 41.0 24-25 37.4913939185586 40.0 37.0 41.0 32.0 41.0 26-27 37.4226291825813 40.0 37.0 41.0 32.0 41.0 28-29 37.47730016593624 40.0 37.0 41.0 32.0 41.0 30-31 37.3095333483118 40.0 37.0 41.0 32.0 41.0 32-33 37.06955381287914 39.0 36.5 41.0 31.0 41.0 34-35 37.117728108191514 40.0 37.0 41.0 31.0 41.0 36-37 37.03139659844242 40.0 36.5 41.0 31.0 41.0 38-39 36.89786597332568 40.0 36.0 41.0 31.0 41.0 40-41 36.67918271664425 39.5 36.0 41.0 30.5 41.0 42-43 36.43836740936443 39.0 36.0 41.0 30.0 41.0 44-45 36.217425876849866 39.0 35.5 41.0 30.0 41.0 46-47 35.87561346330318 39.0 35.0 41.0 28.5 41.0 48-49 35.517503837106375 39.0 35.0 40.0 27.0 41.0 50-51 34.60568013556423 38.0 34.0 39.5 25.0 40.5 52-53 34.73250766744537 38.0 34.5 39.5 26.0 40.5 54-55 35.08211475316646 39.0 35.0 40.0 26.0 41.0 56-57 34.832009858724106 38.0 34.5 40.0 25.5 41.0 58-59 34.57106158589569 38.0 34.0 40.0 25.0 41.0 60-61 34.2090640979484 38.0 34.0 40.0 23.0 41.0 62-63 33.84957189532753 37.5 33.5 40.0 22.5 41.0 64-65 33.473600437443665 37.0 33.0 40.0 22.0 41.0 66-67 33.02212122278497 36.0 32.5 39.0 20.5 41.0 68-69 32.51211903015275 36.0 32.0 39.0 18.5 40.0 70-71 32.03996613601358 35.0 31.0 38.5 16.5 40.0 72-73 31.56967900947163 35.0 31.0 37.5 12.0 40.0 74-75 31.090858889228763 35.0 31.0 37.0 7.5 39.0 76-77 30.58081609229628 34.5 30.0 36.5 6.5 39.0 78-79 30.016075242475345 34.0 29.5 36.0 2.0 38.5 80-81 29.444924327119068 34.0 29.0 35.5 2.0 37.5 82-83 28.894050522577345 34.0 29.0 35.0 2.0 37.0 84-85 28.395163757142974 34.0 27.5 35.0 2.0 36.5 86-87 27.928924879337554 33.5 26.5 35.0 2.0 36.0 88-89 27.417483940999244 33.0 26.0 35.0 2.0 35.5 90-91 26.873193447007328 33.0 25.0 35.0 2.0 35.0 92-93 26.284854731348414 32.5 24.5 35.0 2.0 35.0 94-95 25.60950506064929 32.0 21.5 34.0 2.0 35.0 96-97 24.844635089153506 32.0 18.0 34.0 2.0 35.0 98-99 23.864293661127338 31.0 2.0 34.0 2.0 35.0 100-101 21.498109193084275 28.0 2.0 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.23210096623095566 1101 2 0.1278335878637762 1101 3 0.13945926938795594 1101 4 0.08582198396386786 1101 5 0.12476633001287496 1101 6 0.23046973474414756 1101 7 0.1293708730780736 1101 8 0.17064730294441688 1101 9 0.2848483561247974 1101 10-11 0.28887007327848124 1101 12-13 0.22152462245378501 1101 14-15 0.35291180977183245 1101 16-17 0.5586863558099253 1101 18-19 0.46242912457029917 1101 20-21 0.6761837304030323 1101 22-23 0.48920765366471386 1101 24-25 0.5165097318448133 1101 26-27 0.42339347909226177 1101 28-29 0.3227711639128472 1101 30-31 0.34374300307933225 1101 32-33 0.24534747396548084 1101 34-35 0.5504841872868482 1101 36-37 0.24696182968032332 1101 38-39 0.3593721940831216 1101 40-41 0.4068541772446892 1101 42-43 0.39774887623999433 1101 44-45 0.5001267422703322 1101 46-47 0.6099496422404513 1101 48-49 0.5833327838406817 1101 50-51 0.5022243147839376 1101 52-53 0.4779586283949797 1101 54-55 0.49176744994591104 1101 56-57 0.21695126913157736 1101 58-59 0.3395911386692134 1101 60-61 0.34341794482543264 1101 62-63 0.4644365092060809 1101 64-65 0.33351958018175054 1101 66-67 0.48170697141946306 1101 68-69 0.5827359973313051 1101 70-71 0.40168021223956885 1101 72-73 0.5388459859232562 1101 74-75 0.5906044062735383 1101 76-77 0.9450926857550321 1101 78-79 1.1366975870748064 1101 80-81 1.1097861513474285 1101 82-83 0.9169591423255774 1101 84-85 1.16770899629363 1101 86-87 1.4655311523774408 1101 88-89 1.2851049369618863 1101 90-91 1.2359838477085674 1101 92-93 1.514487071744366 1101 94-95 1.5533400643577622 1101 96-97 1.515697171209169 1101 98-99 1.6311971948395296 1101 100-101 1.7663101452281644 1102 1 0.2402517394268351 1102 2 0.0438344469703047 1102 3 0.2036023106250724 1102 4 0.22304277434187725 1102 5 0.18217612382730408 1102 6 -0.031321502369259235 1102 7 0.09591941259697023 1102 8 0.19972805895816492 1102 9 0.18074612244782173 1102 10-11 0.14202192035752859 1102 12-13 0.36327719977337125 1102 14-15 0.32938839911890483 1102 16-17 0.2264859692119856 1102 18-19 0.20959407302390787 1102 20-21 0.22164120462983306 1102 22-23 0.5398143976509786 1102 24-25 0.5046701699891472 1102 26-27 0.41623067840497896 1102 28-29 0.3471429422633676 1102 30-31 0.4458155975810456 1102 32-33 0.3972536165771672 1102 34-35 0.3982344020634798 1102 36-37 0.44166758569406994 1102 38-39 0.6579117129834628 1102 40-41 0.699009460749842 1102 42-43 0.7204507662743538 1102 44-45 0.7305015274937006 1102 46-47 0.8589401920686299 1102 48-49 0.6484584281705779 1102 50-51 0.7128235415880582 1102 52-53 0.5286512830341508 1102 54-55 0.7782633691898937 1102 56-57 0.6842676351109631 1102 58-59 0.7911751163324467 1102 60-61 0.8000169568529216 1102 62-63 1.0190595762163923 1102 64-65 1.0237203963329549 1102 66-67 0.9605622119692896 1102 68-69 0.9334393908020999 1102 70-71 0.9855880730643136 1102 72-73 1.1349102041363146 1102 74-75 1.1984759698474186 1102 76-77 1.1316315603254772 1102 78-79 1.177140026937348 1102 80-81 1.2555604211068783 1102 82-83 1.172210430985377 1102 84-85 1.0205279395925935 1102 86-87 1.1050715303843113 1102 88-89 0.9785274025976243 1102 90-91 1.2284290796673325 1102 92-93 1.6974552848027855 1102 94-95 1.7557616708869723 1102 96-97 1.852697815539063 1102 98-99 1.811163904461523 1102 100-101 2.2976729029601195 1103 1 0.2333517041294222 1103 2 0.17790983737184263 1103 3 0.19710758697945607 1103 4 0.28372389266123577 1103 5 0.22551160746269971 1103 6 0.3328236308480399 1103 7 0.3700573646168337 1103 8 0.33917839306641895 1103 9 0.31078056785246133 1103 10-11 0.1835012470289783 1103 12-13 0.14041482316216758 1103 14-15 0.46700874503354584 1103 16-17 0.43725593963519316 1103 18-19 0.3784562792338164 1103 20-21 0.2032682935671417 1103 22-23 0.14894729848959543 1103 24-25 0.3874346875709094 1103 26-27 0.10853097417293611 1103 28-29 0.10926577725405906 1103 30-31 0.060605212839263345 1103 32-33 0.31200245723977815 1103 34-35 0.14223140786929633 1103 36-37 0.21978379937729642 1103 38-39 0.45147176118268817 1103 40-41 0.6755506796059692 1103 42-43 0.421201749124684 1103 44-45 0.534438405985405 1103 46-47 0.5328121963530066 1103 48-49 0.31327116931883836 1103 50-51 0.3109868590578415 1103 52-53 0.4467399890797452 1103 54-55 0.3201833049242637 1103 56-57 0.31003900525909245 1103 58-59 0.4991991916011287 1103 60-61 0.5815150276668319 1103 62-63 0.7373132063725549 1103 64-65 0.6924438472026111 1103 66-67 0.6560490119546785 1103 68-69 0.836915577221113 1103 70-71 0.8904475528535016 1103 72-73 0.859731587064541 1103 74-75 0.8776407600712552 1103 76-77 0.8770258567507021 1103 78-79 1.0617984331983799 1103 80-81 1.1219682400920163 1103 82-83 1.1785183217824873 1103 84-85 1.0599186332475803 1103 86-87 0.9370753278988886 1103 88-89 1.1585362878080083 1103 90-91 0.9702315602233256 1103 92-93 1.0162998423071379 1103 94-95 1.1100776922601625 1103 96-97 1.1847478648337244 1103 98-99 1.2895618888576337 1103 100-101 1.7978128754524825 1104 1 0.3475634174663291 1104 2 0.29838246085900977 1104 3 0.2511912264750791 1104 4 0.37270158951220367 1104 5 0.33226795550051946 1104 6 0.28847526140214086 1104 7 0.099663731769013 1104 8 0.4004533280852911 1104 9 0.42659358802769987 1104 10-11 0.39772017622603784 1104 12-13 0.4518237121807047 1104 14-15 0.6024803782590453 1104 16-17 0.6893450846785854 1104 18-19 0.5354135198888983 1104 20-21 0.45083831427818666 1104 22-23 0.6529382832702666 1104 24-25 0.62789017722843 1104 26-27 0.5265211882383412 1104 28-29 0.6752601918712386 1104 30-31 0.7746936424378035 1104 32-33 0.6499442175718926 1104 34-35 0.8281587917515196 1104 36-37 0.8213915544310808 1104 38-39 1.150085241330629 1104 40-41 1.1201689529273935 1104 42-43 1.1081547063223454 1104 44-45 1.2059087372855046 1104 46-47 1.0584569027519422 1104 48-49 0.7161249652451076 1104 50-51 0.9196861158242484 1104 52-53 0.894365894324757 1104 54-55 1.036943138069013 1104 56-57 1.148979471342244 1104 58-59 1.2778273491156824 1104 60-61 1.261155401479094 1104 62-63 1.4312095098562807 1104 64-65 1.0919714386559676 1104 66-67 1.3036171903184623 1104 68-69 1.3284684149732655 1104 70-71 1.190157536686904 1104 72-73 1.3320426428369991 1104 74-75 1.426057646412243 1104 76-77 1.4678420626514352 1104 78-79 1.3826961783188487 1104 80-81 1.3032713323183849 1104 82-83 1.5319258198288317 1104 84-85 1.4010944806889434 1104 86-87 1.8205837098113378 1104 88-89 2.0747824131213974 1104 90-91 2.1302194621428967 1104 92-93 1.9780734393213066 1104 94-95 1.8489641161566333 1104 96-97 2.2858389950063085 1104 98-99 2.472558405285998 1104 100-101 2.7423728077769276 1105 1 0.23661485511984282 1105 2 0.02281815576501245 1105 3 0.14621081259782898 1105 4 0.16753494692683546 1105 5 -0.11759478109824073 1105 6 -0.14044075908301323 1105 7 0.13581377431263775 1105 8 0.1442584140555283 1105 9 -0.03127818708507846 1105 10-11 0.18981528932787484 1105 12-13 0.08525919035501772 1105 14-15 0.18178912458665053 1105 16-17 0.12552663358770388 1105 18-19 0.06042295173079282 1105 20-21 0.24960194027957527 1105 22-23 0.19898774625730908 1105 24-25 0.28026358986950584 1105 26-27 0.18338576304287812 1105 28-29 0.030506503419026387 1105 30-31 -0.13484495988363676 1105 32-33 -0.017441877886376744 1105 34-35 0.20756366259548287 1105 36-37 0.12045720005069427 1105 38-39 0.3735697249473162 1105 40-41 0.43897223280024633 1105 42-43 0.4482889996967785 1105 44-45 0.23274634103576375 1105 46-47 0.2827119879194626 1105 48-49 0.24604497519870705 1105 50-51 -0.023200067932116042 1105 52-53 0.2783251407406624 1105 54-55 0.23835309809405913 1105 56-57 0.25250296666244054 1105 58-59 0.3230209226198326 1105 60-61 0.30296655593654265 1105 62-63 -0.006570435238359096 1105 64-65 0.145961709811381 1105 66-67 0.11291839117254909 1105 68-69 0.1563663985658721 1105 70-71 0.6299402431037677 1105 72-73 0.4566121896269628 1105 74-75 0.32883743096489226 1105 76-77 0.3279437660019475 1105 78-79 0.020108080901966474 1105 80-81 0.3171742686313799 1105 82-83 0.5928464880045929 1105 84-85 0.6531835333306653 1105 86-87 0.7132934980564549 1105 88-89 0.9430717579495393 1105 90-91 0.9621219649925159 1105 92-93 1.4741321334727608 1105 94-95 1.5311371322589942 1105 96-97 1.5561485600980518 1105 98-99 1.7862704973086672 1105 100-101 2.135349497080014 1106 1 0.18966811499128866 1106 2 0.412148326155787 1106 3 0.3472846826110967 1106 4 0.36235435310987896 1106 5 0.23401219778146753 1106 6 0.10944700747141667 1106 7 0.11763705765107346 1106 8 0.39395997512072256 1106 9 0.23569202003664458 1106 10-11 0.18550833085424046 1106 12-13 0.05623536625543579 1106 14-15 0.18188477809139414 1106 16-17 5.381120082716961E-4 1106 18-19 0.46676796754550054 1106 20-21 0.3450628626344354 1106 22-23 0.38401223355452174 1106 24-25 0.36228710787786866 1106 26-27 0.37157701903716855 1106 28-29 0.30501548209466733 1106 30-31 0.23652020693607767 1106 32-33 0.28354909242278126 1106 34-35 0.3062219627689373 1106 36-37 0.4602796671458833 1106 38-39 0.4160526348544664 1106 40-41 0.21864394997196257 1106 42-43 -0.02413473257544041 1106 44-45 0.2853238841436152 1106 46-47 0.4541817359043634 1106 48-49 0.5740740028489455 1106 50-51 0.36588650486658025 1106 52-53 0.17779075649414722 1106 54-55 0.48724351743902616 1106 56-57 0.6259776121067873 1106 58-59 0.49447664142403625 1106 60-61 0.7058361610788708 1106 62-63 0.8773368191234425 1106 64-65 0.8606846972616395 1106 66-67 0.881078527893294 1106 68-69 0.7493122714359899 1106 70-71 0.8977675056280034 1106 72-73 0.9202982930857893 1106 74-75 1.2632369820311133 1106 76-77 1.107132114129687 1106 78-79 1.2583745843199843 1106 80-81 1.1784027147083087 1106 82-83 1.3048465390197954 1106 84-85 0.7989977359630487 1106 86-87 1.148881703341626 1106 88-89 1.1751832771822706 1106 90-91 1.7015184551465836 1106 92-93 1.6173624160969844 1106 94-95 1.9444342447985328 1106 96-97 2.0374042993083386 1106 98-99 2.183422573627407 1106 100-101 2.590729050186841 1107 1 0.283055033872639 1107 2 0.44006317348230084 1107 3 0.40685529315395286 1107 4 0.3902057438663675 1107 5 0.37834265542420553 1107 6 0.18443454278801852 1107 7 0.1737931003784965 1107 8 0.35436911891029155 1107 9 0.3348293128584956 1107 10-11 0.3337217262340033 1107 12-13 0.27326136834043524 1107 14-15 0.431835674793156 1107 16-17 0.39356695453021473 1107 18-19 0.35251139148071786 1107 20-21 0.2468018748271632 1107 22-23 0.43645304519427697 1107 24-25 0.45655486438595005 1107 26-27 0.40922338463766295 1107 28-29 0.4140731520298502 1107 30-31 0.46781940466372873 1107 32-33 0.22155957786554126 1107 34-35 0.3615968853368159 1107 36-37 0.3987766529588157 1107 38-39 0.2506122915846447 1107 40-41 0.0656607357456025 1107 42-43 0.34060003407058304 1107 44-45 0.33679169504302564 1107 46-47 0.7882669792300945 1107 48-49 0.9135001741514728 1107 50-51 0.9804407224621841 1107 52-53 0.6715629474077787 1107 54-55 1.006643097416962 1107 56-57 0.9060537599908116 1107 58-59 0.862615399790478 1107 60-61 0.9131381992434697 1107 62-63 1.0596747404675142 1107 64-65 0.8457044141244694 1107 66-67 1.0383435497253686 1107 68-69 0.9937439574166191 1107 70-71 1.200157703098803 1107 72-73 1.3036016608166143 1107 74-75 1.3192283398506213 1107 76-77 1.2898205574795902 1107 78-79 1.4679323524617658 1107 80-81 1.4512614331877707 1107 82-83 1.2638112904060215 1107 84-85 1.4724079222534066 1107 86-87 1.5062494643823143 1107 88-89 1.4545795332446403 1107 90-91 1.5042682398475087 1107 92-93 1.4663342685755687 1107 94-95 1.3907953126819557 1107 96-97 1.9799881904047787 1107 98-99 2.5125566936207626 1107 100-101 2.440033294222676 1108 1 0.4104654298324881 1108 2 0.3289590667909792 1108 3 0.3314492119166914 1108 4 0.27976355484509696 1108 5 0.30002973997456195 1108 6 0.3313557500698181 1108 7 0.3217439573692573 1108 8 0.31331971673751724 1108 9 0.28923054003283966 1108 10-11 0.3298482820907438 1108 12-13 0.40696523548060526 1108 14-15 0.39930189597873067 1108 16-17 0.5955936833961317 1108 18-19 0.5816819981335186 1108 20-21 0.4566315273378976 1108 22-23 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-1.2972389626440979 2316 96-97 -1.1214973120310567 2316 98-99 -1.0070040388662456 2316 100-101 -1.2982062653863622 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 3395.0 3 716.0 4 644.0 5 715.0 6 764.0 7 895.0 8 1149.0 9 1357.0 10 1613.0 11 2040.0 12 2657.0 13 3267.0 14 3661.0 15 4509.0 16 5698.0 17 7203.0 18 6603.0 19 4963.0 20 4861.0 21 5362.0 22 5628.0 23 6360.0 24 6961.0 25 8122.0 26 9353.0 27 10818.0 28 12679.0 29 15476.0 30 18529.0 31 23120.0 32 29773.0 33 39132.0 34 52836.0 35 74436.0 36 106859.0 37 141447.0 38 102725.0 39 12510.0 40 2.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.47776912240894 18.330940289716608 14.512565514658185 22.678725073216278 2 15.094795314575535 19.57533761460172 41.092713928866544 24.237153141956206 3 20.12793466242479 23.636437636939608 29.865621120751058 26.370006579884546 4 12.537306143711405 15.916428811738276 37.71162757887197 33.83463746567835 5 13.552265855289663 36.972366427332155 34.82459579236092 14.650771925017262 6 31.908445184544327 35.513358411147784 17.537338732205285 15.040857672102604 7 28.35602321418886 32.3820306991376 20.895888702066497 18.36605738460704 8 25.878265397868972 34.58816286068043 19.779194484337346 19.754377257113255 9 25.287671195722194 17.451080640152323 21.347677678071353 35.913570486054134 10-11 25.15593840712621 26.091195913155918 27.322565180864146 21.430300498853725 12-13 26.099518437301246 23.562194276849052 27.98610235275451 22.352184933095195 14-15 22.713490292012573 24.970777045810202 26.880371667159363 25.43536099501786 16-17 21.800005695448117 28.48320724244031 27.420192533267517 22.296594528844054 18-19 21.562598314149646 27.436360233892753 29.52665466103993 21.47438679091767 20-21 22.436234235248097 25.896666343513118 29.573227158185468 22.093872263053314 22-23 21.89977514044401 25.910165997466006 29.798602576157023 22.391456285932957 24-25 22.401815166975567 25.907278429096902 29.699361274901264 21.991545129026267 26-27 21.84885515228929 25.872636200673107 30.301039488635595 21.977469158402013 28-29 21.46725477100207 26.911469051630903 29.85377628755183 21.767499889815195 30-31 21.6804609999044 26.05322056491337 29.704769401734783 22.561549033447445 32-33 21.687843305304995 26.152668432761487 29.910694004747707 22.248794257185807 34-35 22.458431845040945 25.996242373041095 29.59215983542923 21.953165946488735 36-37 22.039721666314136 25.804371878101563 30.11740507530521 22.038501380279094 38-39 21.710105724871276 26.531824237062345 29.91949452732271 21.83857551074367 40-41 21.865009531195344 26.61840510427372 29.720653273280927 21.795932091250013 42-43 21.527097436870957 26.506571386923667 29.179873284269693 22.786457891935687 44-45 21.836602423818398 26.307568398701587 29.190767904066906 22.665061273413116 46-47 22.334929021157045 26.61318479719217 29.005157228696348 22.046728952954442 48-49 22.18873589138767 26.569448578443104 29.007720539129174 22.234094991040052 50-51 21.51009359938595 26.94569282802503 29.42779882777008 22.116414744818943 52-53 22.05466010522982 27.2650214250307 28.29880712861189 22.381511341127585 54-55 21.68115973471787 26.71581156887179 28.771124330319914 22.831904366090424 56-57 22.167620576249956 26.891980923615836 28.458882475266584 22.481516024867624 58-59 22.661619845287447 26.67110432658285 28.441378150987084 22.225897677142616 60-61 22.139181564838484 26.678692309621667 29.41903400328502 21.76309212225483 62-63 21.768390652646993 26.93546746934044 29.274814076620093 22.021327801392477 64-65 21.616446044804043 27.434545533424075 28.738211959675276 22.210796462096603 66-67 21.805251351884504 27.044740140581077 28.269757308175436 22.880251199358987 68-69 21.56340883052789 26.933720197137838 28.843815037522624 22.659055934811644 70-71 22.240013015449065 26.804910620470864 28.355850512144364 22.59922585193571 72-73 21.983926203704456 26.544133796458237 29.10406481579397 22.36787518404334 74-75 21.44551883466924 27.61715402887296 28.40825894551883 22.52906819093897 76-77 21.765584087152373 27.01056454051503 28.309537918306855 22.914313454025738 78-79 22.004442383265395 26.987681743106577 28.438846716080725 22.569029157547305 80-81 22.252165765648495 26.970951825019423 28.215563682120248 22.561318727211834 82-83 22.004885334302354 26.73842580508696 28.85221762042322 22.40447124018747 84-85 21.85688069911648 27.175773615552373 28.33229047961554 22.635055205715606 86-87 21.73759480661755 27.323423098512105 28.507200828685086 22.431781266185254 88-89 21.763327633967858 26.976077580070207 28.061216880065192 23.199377905896743 90-91 21.842648699880275 26.75119896841937 28.223519984162905 23.182632347537453 92-93 22.223404846711528 26.72416481476711 28.525512825901554 22.52691751261981 94-95 21.921395662891065 26.487033958744043 28.603726974192657 22.98784340417224 96-97 21.296435635778945 26.738290173493475 29.17102196698242 22.794252223745165 98-99 21.589465478086975 27.101176151577803 28.210758227976818 23.098600142358407 100-101 21.668962375679225 26.30929979067472 28.78710643051594 23.23463140313012 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 879.0 1 515.5 2 170.5 3 184.0 4 147.5 5 139.5 6 230.0 7 452.0 8 754.0 9 934.0 10 885.5 11 813.5 12 888.5 13 929.5 14 975.0 15 1065.0 16 1084.0 17 1131.0 18 1197.5 19 1265.5 20 1380.0 21 1596.0 22 1963.0 23 2382.5 24 2770.0 25 3387.0 26 4353.5 27 5408.0 28 6243.0 29 7177.0 30 8433.5 31 9571.5 32 10915.0 33 12427.5 34 13857.5 35 15142.0 36 16614.5 37 18404.0 38 19910.0 39 20905.0 40 21711.0 41 22890.0 42 23780.0 43 24585.0 44 25744.5 45 26884.0 46 27669.5 47 30778.5 48 36607.0 49 36105.0 50 30655.0 51 27386.0 52 25456.0 53 24386.0 54 23617.5 55 21896.5 56 19734.5 57 17780.0 58 15683.0 59 13569.0 60 11127.0 61 8838.5 62 6853.0 63 4987.0 64 3554.5 65 2609.5 66 1892.0 67 1398.0 68 1059.5 69 746.0 70 521.5 71 360.5 72 268.0 73 203.0 74 140.5 75 89.5 76 70.0 77 45.0 78 20.0 79 16.0 80 11.5 81 9.5 82 11.0 83 8.0 84 4.5 85 4.5 86 5.5 87 4.0 88 1.5 89 3.5 90 3.5 91 2.0 92 1.5 93 2.0 94 2.5 95 2.0 96 1.5 97 0.5 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.2679883817562172 2 0.2475508839556168 3 0.2359109845460033 4 0.2292789488358747 5 0.22400038980128256 6 0.18948673457510307 7 0.18380213253784997 8 0.19584807495012438 9 0.19638946562033896 10-11 0.19591574878390122 12-13 0.19584807495012438 14-15 0.19002812524531765 16-17 0.19043416824797857 18-19 0.19029882058042494 20-21 0.17791450899926642 22-23 0.17141782095669147 24-25 0.17256827613089742 26-27 0.18407282787295726 28-29 0.1949683151110257 30-31 0.18914836540621896 32-33 0.1934794907679356 34-35 0.19009579907909446 36-37 0.1767640538250604 38-39 0.17737311832905184 40-41 0.17053806111759276 42-43 0.1841405017067341 44-45 0.17920031184102606 46-47 0.1799447240125711 48-49 0.1880655840657898 50-51 0.19490064127724885 52-53 0.20295382749669072 54-55 0.18393748020540363 56-57 0.158627466372872 58-59 0.1640413730750178 60-61 0.1688462152731722 62-63 0.1763580108223995 64-65 0.16593624042076882 66-67 0.170064344281155 68-69 0.17365105747132661 70-71 0.16959062744471726 72-73 0.16783110776651985 74-75 0.17080875645270005 76-77 0.18556165221604734 78-79 0.18894534390488849 80-81 0.18123052685433072 82-83 0.17926798567480284 84-85 0.1730419929673352 86-87 0.17378640513888025 88-89 0.17987705017879427 90-91 0.17987705017879427 92-93 0.17689940149261407 94-95 0.175410577149524 96-97 0.16661297875853706 98-99 0.17094410412025368 100-101 0.1664776310909834 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 738838.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 70.8896571924169 #Duplication Level Percentage of deduplicated Percentage of total 1 86.91274264459268 61.6121453172793 2 8.14000061070814 11.540837056783287 3 2.0546705083195596 4.369646639344277 4 0.9230151877293544 2.617289209661131 5 0.5238902857331638 1.856920138103066 6 0.3025133317184981 1.2867039828996136 7 0.2137121804344133 1.0604988246187652 8 0.14802902575024215 0.8394981511969697 9 0.12640599329565108 0.8064789778616087 >10 0.6091083517143305 7.541954292083274 >50 0.03232990980953856 1.5889648313877096 >100 0.009947358458134782 1.270110346501566 >500 0.0019129535454273446 0.9522437370858687 >1k 0.00172165819088461 2.6567084951935707 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 3603 0.4876576461957831 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 3033 0.4105094756902054 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2880 0.3898012825544977 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2468 0.33403804352239597 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 1909 0.25837869735990837 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 1870 0.25310013832531625 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1515 0.20505171634377226 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 1277 0.17283897146600471 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 1036 0.14022018358557628 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 929 0.12573798315733625 No Hit NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 923 0.1249258971520144 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 855 0.1157222557583665 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 751 0.10164609833278743 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 6.767383377682253E-5 0.0 12-13 0.0 0.0 0.0 2.030215013304676E-4 0.0 14-15 0.0 0.0 0.0 2.706953351072901E-4 0.0 16-17 0.0 0.0 0.0 2.706953351072901E-4 0.0 18-19 0.0 0.0 0.0 2.706953351072901E-4 0.0 20-21 0.0 0.0 0.0 3.383691688841126E-4 0.0 22-23 0.0 0.0 0.0 6.090645039914027E-4 0.0 24-25 0.0 0.0 0.0 8.797598390986928E-4 0.0 26-27 0.0 0.0 0.0 0.0017595196781973856 0.0 28-29 0.0 0.0 0.0 0.002842301018626546 0.0 30-31 0.0 0.0 0.0 0.005752275871029914 0.0 32-33 0.0 0.0 0.0 0.008797598390986927 0.0 34-35 0.0 0.0 0.0 0.012181290079828055 0.0 36-37 0.0 0.0 0.0 0.017662870615750677 0.0 38-39 0.0 0.0 0.0 0.02233236514635143 0.0 40-41 0.0 0.0 0.0 0.027272555012059475 0.0 42-43 0.0 0.0 0.0 0.03708526090969874 0.0 44-45 0.0 0.0 0.0 0.05298861184725204 0.0 46-47 0.0 0.0 0.0 0.08601344273034142 0.0 48-49 0.0 0.0 0.0 0.15158938766008245 1.3534766755364506E-4 50-51 0.0 0.0 0.0 0.1907725374168627 1.3534766755364506E-4 52-53 0.0 0.0 0.0 0.2259629309808104 1.3534766755364506E-4 54-55 0.0 0.0 0.0 0.2746880913001226 1.3534766755364506E-4 56-57 0.0 0.0 0.0 0.3177286495821817 1.3534766755364506E-4 58-59 0.0 0.0 0.0 0.3684163510810218 1.3534766755364506E-4 60-61 0.0 0.0 0.0 0.44657962909325183 1.3534766755364506E-4 62-63 0.0 0.0 0.0 0.5058619074817483 1.3534766755364506E-4 64-65 0.0 0.0 0.0 0.5754306086043219 1.3534766755364506E-4 66-67 0.0 0.0 0.0 0.677550423773547 1.3534766755364506E-4 68-69 0.0 0.0 0.0 0.7897536401755187 1.3534766755364506E-4 70-71 0.0 0.0 0.0 0.9016861612423833 1.3534766755364506E-4 72-73 0.0 0.0 0.0 1.026476710726844 1.3534766755364506E-4 74-75 0.0 0.0 0.0 1.1390182962976998 1.3534766755364506E-4 76-77 0.0 0.0 0.0 1.241950197472247 1.3534766755364506E-4 78-79 0.0 0.0 0.0 1.375741366849025 1.3534766755364506E-4 80-81 0.0 0.0 0.0 1.533286051881468 2.030215013304676E-4 82-83 0.0 0.0 0.0 1.6927932780934385 2.706953351072901E-4 84-85 0.0 0.0 0.0 1.8592032353506451 2.706953351072901E-4 86-87 0.0 0.0 0.0 2.151824892601626 2.706953351072901E-4 88-89 0.0 0.0 0.0 2.577222611722732 2.706953351072901E-4 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGACCGT 95 3.4881457E-5 30.001286 6 TATACCG 65 0.0058299056 29.24392 5 TAGCGTG 65 0.0058415104 29.232021 9 GACCGTC 85 6.4033805E-4 27.942373 7 GTACATG 2345 0.0 26.957903 1 TACCTGG 600 0.0 26.136751 2 GTACCTG 620 0.0 26.065409 1 TACATGG 2395 0.0 25.794477 2 ACCGTCC 115 1.280772E-4 24.78367 8 TGGACCG 135 1.33721005E-5 24.64071 5 ACATGGG 2400 0.0 23.955439 3 GAGTACT 2580 0.0 22.828888 12-13 GTACTAG 150 3.0160583E-5 22.18115 1 GTAAGCG 75 3.0369341E-5 22.163858 94-95 TACCGCA 160 4.9845323E-5 20.782139 7 GTACTTT 2820 0.0 20.547739 14-15 GTGTAGC 325 2.1827873E-11 20.47491 1 GTCTAGA 350 3.6379788E-12 20.370445 1 AGAGTAC 3730 0.0 19.738113 10-11 GAGTACA 1835 0.0 19.167831 1 >>END_MODULE