FastQCFastQC Report
Thu 26 May 2016
SRR937685_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937685_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences738838
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT61200.8283277254283078No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT38690.5236601257650527No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT34620.46857362507071915No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT23490.3179316710835122No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT17700.23956537156995172No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT13500.18271935119742083No Hit
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT13220.17892961650591876No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC12650.17121479945536097No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT12310.16661297875853706No Hit
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA12230.1655301974181079No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC11980.16214650572926675No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC11710.15849211870531835No Hit
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG11390.1541609933436017No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9940.13453558154832318No Hit
GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA7980.10800743870780875No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCGTC500.001615357638.0004047
TATACGG908.936883E-426.3927445
GTACCTA950.001226547424.9985756
GACGCGC500.001654417323.74864660-61
TCGACGC500.001654417323.74864658-59
GTACATG25250.023.7160631
TACACCG1050.002193621422.622355
TACCTGG5850.021.9262772
ACAGTCG1754.040885E-621.7174575
TACATGG26250.021.7174572
ACGATTC1100.002873967221.5940635
AGTACTT37200.021.00492912-13
ACATGGG25050.020.861333
AGAGTAC48500.020.7631110-11
TAGGGCG1150.003718888420.6551915
AGGACCG2551.1881639E-820.493195
GGTGTGC5850.020.2993628
GAGTACT36850.020.17321412-13
CACTAGG1908.234296E-620.0029222
TATAGGG2151.076216E-619.8866252
Produced by FastQC (version 0.11.5)