##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937685_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 738838 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.462962110773944 33.0 31.0 34.0 31.0 34.0 2 32.67478256397207 34.0 31.0 34.0 31.0 34.0 3 32.73955454375655 34.0 31.0 34.0 31.0 34.0 4 36.26209399083426 37.0 35.0 37.0 35.0 37.0 5 36.206060056467045 37.0 35.0 37.0 35.0 37.0 6 36.269195682950794 37.0 37.0 37.0 35.0 37.0 7 36.235510355450046 37.0 37.0 37.0 35.0 37.0 8 36.20712930304072 37.0 37.0 37.0 35.0 37.0 9 37.93977299489198 39.0 38.0 39.0 35.0 39.0 10-11 37.85909563395494 39.0 38.0 39.0 35.0 39.0 12-13 37.76561370692899 39.0 37.5 39.0 35.0 39.0 14-15 39.02857121588224 40.0 38.0 41.0 36.0 41.0 16-17 39.0062158416324 40.0 38.0 41.0 36.0 41.0 18-19 38.96725669226542 40.0 38.0 41.0 36.0 41.0 20-21 38.85668915242584 40.0 38.0 41.0 35.0 41.0 22-23 38.81080426291014 40.0 38.0 41.0 35.0 41.0 24-25 38.813733863174335 40.0 38.0 41.0 35.0 41.0 26-27 38.63522382443783 40.0 38.0 41.0 34.5 41.0 28-29 38.61260452223627 40.0 38.0 41.0 34.5 41.0 30-31 38.656921409023354 40.0 38.0 41.0 35.0 41.0 32-33 38.533996627136126 40.0 38.0 41.0 35.0 41.0 34-35 38.36739785988268 40.0 38.0 41.0 34.0 41.0 36-37 38.2003165781944 40.0 38.0 41.0 34.0 41.0 38-39 38.05663961517951 40.0 38.0 41.0 34.0 41.0 40-41 37.896697246216355 40.0 38.0 41.0 33.0 41.0 42-43 37.668778541439394 40.0 37.0 41.0 33.0 41.0 44-45 37.361028398647605 40.0 37.0 41.0 33.0 41.0 46-47 36.99208689861648 40.0 37.0 41.0 31.5 41.0 48-49 36.61571345815997 40.0 36.0 41.0 31.0 41.0 50-51 36.32969338339392 39.0 36.0 41.0 30.0 41.0 52-53 36.08774521613669 39.0 36.0 41.0 30.0 41.0 54-55 35.85081167996232 39.0 35.0 40.0 29.0 41.0 56-57 35.55052528429778 39.0 35.0 40.0 28.0 41.0 58-59 35.27574786353706 38.0 35.0 40.0 28.0 41.0 60-61 34.86871140899629 38.0 34.0 40.0 27.0 41.0 62-63 34.47246419377455 37.5 34.0 40.0 26.0 41.0 64-65 34.079780682639495 37.0 34.0 39.5 26.0 41.0 66-67 33.63187870683424 36.5 33.5 39.0 25.0 40.5 68-69 33.127315460222675 36.0 33.0 39.0 23.0 40.0 70-71 32.67104561487092 35.0 32.0 38.5 22.0 40.0 72-73 32.17713558317249 35.0 32.0 37.5 21.5 39.5 74-75 31.68573692744553 35.0 31.0 37.0 19.5 39.0 76-77 30.487749682609717 34.0 30.0 35.5 19.5 38.0 78-79 31.068991443320456 34.0 31.0 36.0 19.0 38.0 80-81 30.87274070905936 34.0 31.0 36.0 18.0 37.0 82-83 30.468668368438006 34.0 31.0 35.0 15.0 37.0 84-85 30.0584992921317 34.0 30.5 35.0 8.5 36.5 86-87 29.736419891776006 34.0 30.0 35.0 6.5 36.0 88-89 29.351954014276473 34.0 30.0 35.0 2.0 36.0 90-91 28.846243696182384 34.0 29.0 35.0 2.0 35.0 92-93 28.393538908393992 34.0 29.0 35.0 2.0 35.0 94-95 27.886029819798114 33.0 28.0 35.0 2.0 35.0 96-97 27.372683186300648 33.0 27.0 35.0 2.0 35.0 98-99 26.577301113369913 33.0 25.5 35.0 2.0 35.0 100-101 24.114460815496766 30.5 13.0 33.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.20744077581274922 1101 2 0.188865468977383 1101 3 0.1705551178804896 1101 4 0.09691467029536938 1101 5 0.12016914501880649 1101 6 0.15490419916159226 1101 7 0.115192303716249 1101 8 0.2542354163942733 1101 9 0.26904876734752037 1101 10-11 0.2531405829522484 1101 12-13 0.24250292246993155 1101 14-15 0.2186989949984408 1101 16-17 0.3322486748188638 1101 18-19 0.2223405761591053 1101 20-21 0.3556512302175605 1101 22-23 0.3134043517184111 1101 24-25 0.2424578543641971 1101 26-27 0.1275797193775361 1101 28-29 0.3292989487510951 1101 30-31 0.364437074652173 1101 32-33 0.2635727706425044 1101 34-35 0.3199079996987706 1101 36-37 0.2372751464698979 1101 38-39 0.41524973917233154 1101 40-41 0.2935376837584158 1101 42-43 0.20160857723901415 1101 44-45 0.12401188954976305 1101 46-47 0.2799207317919752 1101 48-49 0.45246378291708567 1101 50-51 0.4945315807552433 1101 52-53 0.38040102127369124 1101 54-55 0.3942240691641956 1101 56-57 0.4094140264863029 1101 58-59 0.32655153411661786 1101 60-61 0.3882283428489117 1101 62-63 0.3055030226254942 1101 64-65 0.6904615365710285 1101 66-67 0.636482551107548 1101 68-69 0.8655746435208513 1101 70-71 0.7052267940608985 1101 72-73 0.6348863425129494 1101 74-75 0.6268643119717687 1101 76-77 0.3335931487921613 1101 78-79 0.5375437481392318 1101 80-81 0.6572560392611244 1101 82-83 0.5423133631984278 1101 84-85 0.7546838955307322 1101 86-87 0.740372264465897 1101 88-89 0.6075885663905609 1101 90-91 0.44063865972491456 1101 92-93 0.6017718655252473 1101 94-95 0.7933115069105376 1101 96-97 0.9195107035227856 1101 98-99 0.9574983094055796 1101 100-101 1.2585088877128747 1102 1 0.2040580438539905 1102 2 0.11137406004267092 1102 3 0.10881757492516186 1102 4 0.024298690913923338 1102 5 0.06755960893632817 1102 6 0.0248784259657171 1102 7 0.07035768172311663 1102 8 0.00309710024306753 1102 9 -0.02275750413014066 1102 10-11 0.02728147642303469 1102 12-13 0.07447242418814426 1102 14-15 0.11062339362386808 1102 16-17 0.046912549389311664 1102 18-19 -0.006439492569413119 1102 20-21 0.08853353262305319 1102 22-23 0.09925057164968365 1102 24-25 0.17330514817863474 1102 26-27 0.11654020047719627 1102 28-29 0.25978649170642854 1102 30-31 0.1957944629683297 1102 32-33 0.17627680844319826 1102 34-35 0.15150164231045693 1102 36-37 0.26733313616062304 1102 38-39 0.18253388865687015 1102 40-41 0.23940323358658588 1102 42-43 0.13114036418060238 1102 44-45 -0.2549131534055675 1102 46-47 -0.25173197439358574 1102 48-49 -0.26073424113790367 1102 50-51 -0.1981015807567772 1102 52-53 -0.300183171166168 1102 54-55 -0.31053323324130133 1102 56-57 -0.37140642024909454 1102 58-59 -0.4567656909692914 1102 60-61 -0.49129270526105273 1102 62-63 -0.4776907058968405 1102 64-65 -0.4315530682399711 1102 66-67 -0.3411860485488134 1102 68-69 -0.2934513444516611 1102 70-71 -0.4009319258703741 1102 72-73 -0.2896787348066354 1102 74-75 -0.3559267103650008 1102 76-77 -0.4505617051597284 1102 78-79 -0.28832609722159575 1102 80-81 -0.2705092098797657 1102 82-83 -0.08482903370878958 1102 84-85 -0.08277314914280254 1102 86-87 -0.12342176474372124 1102 88-89 -0.08008325491528012 1102 90-91 -0.13343780075618739 1102 92-93 -0.07254084925138571 1102 94-95 0.19827821653253253 1102 96-97 0.36120958668429637 1102 98-99 0.18034517366675118 1102 100-101 0.5496063963039433 1103 1 0.08462864476198462 1103 2 0.09711025449489341 1103 3 0.10964036974983316 1103 4 0.0518659689966654 1103 5 -0.01884723632711882 1103 6 0.09187291226666616 1103 7 0.1322316091078264 1103 8 0.19405487723646075 1103 9 0.15120761229045598 1103 10-11 0.0308757265964843 1103 12-13 0.1868739870114453 1103 14-15 0.12881312329438543 1103 16-17 0.0825665860742788 1103 18-19 0.10505407478169815 1103 20-21 0.12280245020969005 1103 22-23 0.15034600854642832 1103 24-25 0.07366542520245645 1103 26-27 0.2044441428328696 1103 28-29 0.07490230372945206 1103 30-31 0.18255002231057205 1103 32-33 0.11924106068579476 1103 34-35 0.315542100446514 1103 36-37 0.2726167934592709 1103 38-39 0.29523608805072854 1103 40-41 0.21628586360886004 1103 42-43 0.28767951387813895 1103 44-45 0.20193089289489308 1103 46-47 0.15701082919456866 1103 48-49 0.255018141437354 1103 50-51 -0.01833490881579536 1103 52-53 0.05328571234019819 1103 54-55 0.10525688104928577 1103 56-57 0.11487201546701442 1103 58-59 0.26703571849284913 1103 60-61 0.22958632199097906 1103 62-63 0.04793095454206053 1103 64-65 -0.043184173779231116 1103 66-67 0.17594597594028727 1103 68-69 0.22476455398916784 1103 70-71 0.22973178717384002 1103 72-73 0.06813051311507934 1103 74-75 0.234564897371218 1103 76-77 0.22110360235375381 1103 78-79 0.37604360055953734 1103 80-81 0.3476160107290518 1103 82-83 0.36174407453254886 1103 84-85 0.48551821272868523 1103 86-87 0.6336973579330376 1103 88-89 0.6622766813059471 1103 90-91 0.5332031028575379 1103 92-93 0.708727788390263 1103 94-95 0.8554837570089227 1103 96-97 1.053676632290987 1103 98-99 1.6028649219596929 1103 100-101 1.6351679294287358 1104 1 0.04095426736021324 1104 2 0.08871375679705551 1104 3 0.06744908190302823 1104 4 -0.02316822957459408 1104 5 0.03160851432959788 1104 6 0.0024822225686094157 1104 7 -0.008798539370012293 1104 8 0.10981354816713917 1104 9 0.1769743741962415 1104 10-11 0.23323377757730412 1104 12-13 0.15316747600265757 1104 14-15 0.1258077943048974 1104 16-17 0.10152357187131145 1104 18-19 0.22313164681364128 1104 20-21 0.1835960720034322 1104 22-23 0.17488300364731657 1104 24-25 0.15603677178942377 1104 26-27 0.23971127551104132 1104 28-29 0.21038775455951253 1104 30-31 0.15584366329543542 1104 32-33 0.3164106011583314 1104 34-35 0.27421825110753417 1104 36-37 0.15198987338796854 1104 38-39 0.10689676452994235 1104 40-41 0.204914742709434 1104 42-43 0.4097549794930302 1104 44-45 0.44331523263601724 1104 46-47 0.28912708744866933 1104 48-49 0.3097066849543566 1104 50-51 0.21815262540197722 1104 52-53 0.1437733663105405 1104 54-55 0.04615975403632433 1104 56-57 0.16937555661201031 1104 58-59 0.00869487503555888 1104 60-61 0.09081151363347573 1104 62-63 0.11236935335804787 1104 64-65 0.14230104675741728 1104 66-67 0.1529534819701368 1104 68-69 0.21385094932926307 1104 70-71 0.13286347048048697 1104 72-73 0.1687188360109957 1104 74-75 0.3675915822361837 1104 76-77 0.29814058593997217 1104 78-79 0.11210893019384471 1104 80-81 0.028136105364289676 1104 82-83 0.15117836644940397 1104 84-85 0.3413115504938915 1104 86-87 0.12296261448757306 1104 88-89 0.16252463054313893 1104 90-91 0.03825190203093953 1104 92-93 -0.03263428965463788 1104 94-95 0.34101211917755236 1104 96-97 0.5246404716198825 1104 98-99 0.7508222961849462 1104 100-101 0.65459010791227 1105 1 0.14104417087447985 1105 2 0.09133460477984556 1105 3 0.021210982078017082 1105 4 0.11890541103610985 1105 5 0.07476019440152015 1105 6 0.08499679175418606 1105 7 0.028579649395261697 1105 8 0.08702862627081487 1105 9 0.11476635994011275 1105 10-11 0.1610109533226236 1105 12-13 0.11082853975388218 1105 14-15 0.16129158759103035 1105 16-17 0.2407556192633109 1105 18-19 0.1919393415912083 1105 20-21 0.20001851416817118 1105 22-23 0.3363210183850711 1105 24-25 0.17229967535185864 1105 26-27 0.14686984283433446 1105 28-29 -0.03393407594025888 1105 30-31 0.09761608699785285 1105 32-33 0.24895085706226183 1105 34-35 0.2014666416740809 1105 36-37 0.33283841807483583 1105 38-39 0.19860236262911712 1105 40-41 0.2582367578324849 1105 42-43 0.35538944143655726 1105 44-45 0.6212148216485218 1105 46-47 0.6753489725327171 1105 48-49 0.6628418693368374 1105 50-51 0.5268618276688244 1105 52-53 0.5152775644835685 1105 54-55 0.5954200568185257 1105 56-57 0.6378862487085257 1105 58-59 0.8395337872030382 1105 60-61 0.7942854416143419 1105 62-63 0.6543841954649992 1105 64-65 0.7382238822500398 1105 66-67 0.7753135696260642 1105 68-69 0.5675229459899853 1105 70-71 0.21431244220904233 1105 72-73 0.2471355709080143 1105 74-75 0.4680487255520127 1105 76-77 0.2464982413847565 1105 78-79 0.20887862468243767 1105 80-81 0.4626379837055694 1105 82-83 0.35271073974163514 1105 84-85 0.3324616733085115 1105 86-87 0.6080613076757757 1105 88-89 0.6441626404646357 1105 90-91 0.48948125231750694 1105 92-93 0.7733768037968503 1105 94-95 0.7633539451821427 1105 96-97 1.0318949627820437 1105 98-99 1.3676256242203948 1105 100-101 1.7820042580832478 1106 1 -0.5578743068318772 1106 2 -0.4559003712430041 1106 3 -0.45021795374485407 1106 4 -0.40716590821702425 1106 5 -0.39122031779110955 1106 6 -0.34189332149956897 1106 7 -0.3114519800303128 1106 8 -0.3747762915947064 1106 9 -0.6535149378866407 1106 10-11 -0.8213084528604284 1106 12-13 -0.7903397083840602 1106 14-15 -0.8724855780043157 1106 16-17 -0.7209753265585448 1106 18-19 -0.9651938591025981 1106 20-21 -0.9944348579367102 1106 22-23 -0.9070329761052847 1106 24-25 -0.8112224142308406 1106 26-27 -0.734162704864886 1106 28-29 -1.1803515333425665 1106 30-31 -1.0711689859538893 1106 32-33 -1.3090942403767798 1106 34-35 -1.5386491628356609 1106 36-37 -1.477678365926792 1106 38-39 -1.2610803228111394 1106 40-41 -1.2971734043958634 1106 42-43 -1.4577750315764035 1106 44-45 -1.568080913480543 1106 46-47 -1.6131190409352953 1106 48-49 -2.045099922317071 1106 50-51 -2.1326206231015092 1106 52-53 -2.0589929181202464 1106 54-55 -1.9895483137559111 1106 56-57 -1.821491620896623 1106 58-59 -1.896954836406799 1106 60-61 -1.971594315553297 1106 62-63 -2.4357762473469577 1106 64-65 -2.7251204193518355 1106 66-67 -2.6725782271293674 1106 68-69 -2.7785412311347955 1106 70-71 -2.890102923569959 1106 72-73 -2.820653430214321 1106 74-75 -2.986096259653575 1106 76-77 -2.965791320904806 1106 78-79 -2.8168725741748197 1106 80-81 -3.0640723009592605 1106 82-83 -3.2429784756445486 1106 84-85 -2.987327123753012 1106 86-87 -3.0410369986194965 1106 88-89 -3.1102144639124703 1106 90-91 -2.9989102383467134 1106 92-93 -3.135073864502303 1106 94-95 -3.3430994779379475 1106 96-97 -3.316689365347738 1106 98-99 -3.4755294109800943 1106 100-101 -2.473214597135609 1107 1 0.15701230232097885 1107 2 0.18755529225586542 1107 3 0.168667930190054 1107 4 0.15901476170304818 1107 5 0.1790185508688964 1107 6 0.12083198709577658 1107 7 0.13552099049260136 1107 8 0.1027767021956123 1107 9 0.07846566253338239 1107 10-11 0.13201017466462162 1107 12-13 0.20241113392636123 1107 14-15 0.23216639292653696 1107 16-17 0.24321854837157275 1107 18-19 0.3469444762200453 1107 20-21 0.42859836611030744 1107 22-23 0.25355898304077584 1107 24-25 0.41499270354153595 1107 26-27 0.29458916483763176 1107 28-29 0.41492699567370295 1107 30-31 0.3634163555784369 1107 32-33 0.17685164023421862 1107 34-35 0.10953703687122385 1107 36-37 0.11341202946807272 1107 38-39 0.27060565781950174 1107 40-41 0.1781450116072847 1107 42-43 0.03438203854310018 1107 44-45 0.10612550167651591 1107 46-47 -0.04094021427749084 1107 48-49 -0.23706813359724066 1107 50-51 0.034252025605944425 1107 52-53 -0.05756363442405643 1107 54-55 0.12581875228424622 1107 56-57 0.09387091253139346 1107 58-59 0.33957046312332295 1107 60-61 0.35384572554239213 1107 62-63 0.33159801043780845 1107 64-65 0.1716810673449416 1107 66-67 0.49495013946830824 1107 68-69 0.6085181611929968 1107 70-71 0.5689826974734515 1107 72-73 0.8127685408676086 1107 74-75 0.9028079058169887 1107 76-77 0.7479032112540764 1107 78-79 1.0320992783037646 1107 80-81 0.9071712967260872 1107 82-83 0.9516398028693587 1107 84-85 1.0197966316672336 1107 86-87 1.1527170618455465 1107 88-89 1.1717034353728906 1107 90-91 1.1289879894019386 1107 92-93 0.9922502323747295 1107 94-95 1.0823949545034672 1107 96-97 1.2562584945038964 1107 98-99 1.1746496271996065 1107 100-101 1.6151683179383483 1108 1 0.14444987542960774 1108 2 0.04461259541415785 1108 3 -0.011089997062043722 1108 4 0.03668957450992849 1108 5 0.10823188448240728 1108 6 0.06807469724588344 1108 7 0.15320715045954358 1108 8 0.12140495662416129 1108 9 0.24583277117893942 1108 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2316 58-59 -0.5054986288256558 2316 60-61 -0.7149781282870364 2316 62-63 -0.7131713256054226 2316 64-65 -0.5812679792576532 2316 66-67 -0.5554721742183375 2316 68-69 -0.5694433965163981 2316 70-71 -0.6282025588255138 2316 72-73 -0.6954121910066036 2316 74-75 -0.490457033465848 2316 76-77 -0.39590813146854487 2316 78-79 -0.6237865591233422 2316 80-81 -0.4337384905340329 2316 82-83 -0.3525103099859308 2316 84-85 -0.5174762534450572 2316 86-87 -0.10379382026408024 2316 88-89 -0.011382569549848398 2316 90-91 -0.26628638683002137 2316 92-93 -0.33505308791981747 2316 94-95 -0.2922360955103187 2316 96-97 0.13273812437939725 2316 98-99 -0.05065753323874134 2316 100-101 0.5787661037277729 >>END_MODULE >>Per sequence quality scores pass #Quality Count 3 1.0 4 10.0 5 17.0 6 21.0 7 57.0 8 155.0 9 313.0 10 504.0 11 843.0 12 1464.0 13 2174.0 14 2963.0 15 3935.0 16 5443.0 17 8207.0 18 7705.0 19 4790.0 20 3951.0 21 3952.0 22 3924.0 23 3989.0 24 4516.0 25 5082.0 26 6174.0 27 7536.0 28 9235.0 29 11256.0 30 14223.0 31 18365.0 32 23931.0 33 32445.0 34 46388.0 35 70408.0 36 114177.0 37 174935.0 38 134096.0 39 11653.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.27819535636959 19.128063402738192 14.889840044305005 21.703901196587214 2 15.763130753967717 20.85991787103533 39.843646374442024 23.533305000554925 3 20.893078049585974 24.128157999453194 29.880975261153324 25.09778868980751 4 13.252196698590756 16.809955118591947 37.094429616315395 32.8434185665019 5 13.50569928408708 37.72681066140392 34.11259419283492 14.654895861674092 6 32.037334300618 36.77044223496896 16.581848794999715 14.610374669413323 7 28.68842696233816 33.21797200468845 20.02671762957509 18.06688340339831 8 26.28451703891787 35.09131907129844 18.871254591669622 19.752909298114066 9 25.87942146992981 18.075274958786636 20.287126541948304 35.75817702933525 10-11 25.552827548122863 26.702673657824853 26.555077026357605 21.18942176769468 12-13 26.90802652950731 23.971304818624798 27.29473470492691 21.825933946940985 14-15 22.89108031801288 25.6934537747111 26.6458953112861 24.76957059598992 16-17 21.97802495269599 28.897539108708543 27.056201765474974 22.068234173120494 18-19 21.635866049120374 27.90435792419989 29.490632587928612 20.96914343875112 20-21 22.57752560952254 25.92759020432802 29.88333684110084 21.611547345048603 22-23 22.07455501889152 26.15685613681752 29.82004497691262 21.94854386737834 24-25 22.72514407759211 26.190856452970745 29.927805554126884 21.156193915310258 26-27 22.20923622484198 26.12014942544293 30.47250382361301 21.19811052610208 28-29 21.488715189659256 27.341048286130004 30.096910635130104 21.073325889080635 30-31 22.01957668663496 26.37499695467748 29.781224030166285 21.824202328521274 32-33 21.910824835755605 26.63256356603207 29.821760656598606 21.634850941613724 34-35 22.726700575768977 26.35483015221199 29.810865169360532 21.1076041026585 36-37 22.436034519162575 26.03324689105566 30.149315951036492 21.38140263874527 38-39 21.85351988766136 26.881824487793327 30.247178845145246 21.01747677940007 40-41 22.054270464439696 26.88801920316606 29.68796208633439 21.369748246059853 42-43 21.70966818311965 27.01991605244793 29.200102074591616 22.070313689840802 44-45 22.475427630955636 26.368838635803787 29.098327373524373 22.057406359716204 46-47 22.87125188471627 27.179774185951455 28.695668062552276 21.253305866779996 48-49 22.814541212011292 27.204542809113768 28.515587990872156 21.465327988002784 50-51 21.91393783210934 27.449386739718314 29.16397099228789 21.472704435884456 52-53 22.657959294161437 28.02720489958888 27.76327676924899 21.551559037000693 54-55 22.085761696068694 27.536415289955308 28.12977946451049 22.248043549465514 56-57 22.606444173147562 27.60029938904063 28.169030288101045 21.62422614971076 58-59 23.321485900833473 27.200144009918276 27.91647154044594 21.561898548802308 60-61 22.299543336969673 27.58669694845149 29.04154902698562 21.07221068759322 62-63 22.100785287167145 27.56869570866685 28.89280194034416 21.43771706382184 64-65 21.946827315324875 28.30674653983688 28.231628584344605 21.51479756049364 66-67 22.28303092152813 27.634542348931703 27.87891256269981 22.203514166840364 68-69 22.00380868336496 27.65971701509668 28.128832030837614 22.20764227070075 70-71 22.864958218175026 27.6354221087708 27.859422498572084 21.640197174482093 72-73 22.638589244191554 27.239259485841284 28.444192096237607 21.67795917372956 74-75 21.931600702725092 28.679561690113392 27.581283041749344 21.807554565412175 76-77 22.395031116428772 27.694433691824187 27.678056624050196 22.23247856769684 78-79 22.507369680498297 27.908621375727833 27.64124205847561 21.942766885298266 80-81 22.8463479138864 27.762039851767234 27.663845118957063 21.7277671153893 82-83 22.713670268272974 27.705163655853728 27.904328018223236 21.676838057650063 84-85 22.345200866497528 28.03949182261569 27.84093639120428 21.7743709196825 86-87 22.209672485714055 28.233320430189025 27.607743510756077 21.94926357334084 88-89 22.04854108749144 28.29409153292061 27.110340832496433 22.547026547091516 90-91 22.499020298600804 27.555159388449745 27.3652428339665 22.580577478982953 92-93 22.764408110229827 27.7049213610893 27.622289596924826 21.908380931756042 94-95 22.606038130144903 27.59535919917492 27.688952111288266 22.10965055939191 96-97 21.78806450128445 27.75912987691483 28.418137670233527 22.03466795156719 98-99 22.23802791833343 28.291150847924463 27.076195110265033 22.394626123477078 100-101 21.733921649451045 28.264555442973204 27.2219390742211 22.779583833354657 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 196.0 1 138.0 2 131.0 3 194.0 4 166.0 5 153.0 6 262.0 7 558.0 8 899.5 9 1115.0 10 1123.0 11 1041.0 12 1091.0 13 1167.0 14 1247.5 15 1357.0 16 1437.0 17 1483.0 18 1509.5 19 1560.0 20 1712.0 21 1918.0 22 2130.0 23 2507.0 24 3041.0 25 3628.0 26 4392.0 27 5409.0 28 6328.5 29 7305.5 30 8404.5 31 9518.0 32 10897.0 33 12270.0 34 13639.5 35 14850.0 36 16608.0 37 18296.0 38 19653.0 39 20758.5 40 21434.5 41 22759.0 42 23654.5 43 24391.5 44 25564.5 45 26377.0 46 27020.0 47 30930.5 48 37628.0 49 36201.5 50 29793.0 51 26955.0 52 25419.5 53 24497.0 54 23656.5 55 21844.0 56 19421.0 57 17477.0 58 15625.5 59 13577.5 60 11200.5 61 8893.5 62 6896.5 63 5016.0 64 3512.0 65 2503.0 66 1799.5 67 1406.0 68 1075.0 69 730.5 70 523.5 71 348.0 72 240.0 73 161.0 74 107.5 75 81.5 76 48.0 77 23.5 78 17.5 79 12.0 80 6.0 81 4.5 82 4.0 83 3.0 84 1.5 85 0.0 86 0.5 87 0.5 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.04344660128472006 2 0.0 3 0.0 4 2.706953351072901E-4 5 0.008256207720772348 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.005143211367038512 14-15 0.0 16-17 0.0 18-19 0.0 20-21 2.030215013304676E-4 22-23 0.0019625411795278535 24-25 0.0 26-27 0.0010827813404291604 28-29 0.0017595196781973858 30-31 0.0 32-33 0.0 34-35 0.0 36-37 2.706953351072901E-4 38-39 6.767383377682253E-5 40-41 2.030215013304676E-4 42-43 0.02199399597746732 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 6.767383377682253E-5 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 1.3534766755364506E-4 84-85 2.030215013304676E-4 86-87 0.0 88-89 0.0 90-91 0.012519659248712168 92-93 0.002436258015965611 94-95 0.0 96-97 0.0 98-99 6.090645039914027E-4 100-101 0.016174046272660586 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 738838.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 67.7226381487793 #Duplication Level Percentage of deduplicated Percentage of total 1 85.46148972138509 57.87677544056984 2 8.957966902748261 12.133143022071236 3 2.3873354258250403 4.850299595487334 4 1.018646407735308 2.7594168829044854 5 0.5711925729696685 1.9341333966247538 6 0.33259226007617454 1.3514415168134077 7 0.23688764081401248 1.122985918653576 8 0.17178847564831487 0.9307175019568971 9 0.12898701039736304 0.7861806567930106 >10 0.6750986221600062 8.285690253946912 >50 0.03699237120366898 1.7238044545986746 >100 0.017586204294263014 2.0876446293745334 >500 8.085611159521233E-4 0.3974054002958675 >1k 0.00242568334785637 2.922565584194446 >5k 2.0214027898803082E-4 0.8377957457150222 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 6120 0.8283277254283078 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3869 0.5236601257650527 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3462 0.46857362507071915 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2349 0.3179316710835122 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 1770 0.23956537156995172 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 1350 0.18271935119742083 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 1322 0.17892961650591876 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 1265 0.17121479945536097 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 1231 0.16661297875853706 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 1223 0.1655301974181079 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 1198 0.16214650572926675 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 1171 0.15849211870531835 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 1139 0.1541609933436017 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 994 0.13453558154832318 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 798 0.10800743870780875 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 2.706953351072901E-4 0.0 3 0.0 0.0 0.0 4.0604300266093514E-4 0.0 4 0.0 0.0 0.0 4.0604300266093514E-4 0.0 5 0.0 0.0 0.0 4.0604300266093514E-4 0.0 6 0.0 0.0 0.0 4.0604300266093514E-4 0.0 7 0.0 0.0 0.0 4.0604300266093514E-4 0.0 8 0.0 0.0 0.0 4.0604300266093514E-4 0.0 9 0.0 0.0 0.0 4.0604300266093514E-4 0.0 10-11 0.0 0.0 0.0 6.090645039914027E-4 0.0 12-13 0.0 0.0 0.0 6.767383377682253E-4 0.0 14-15 0.0 0.0 0.0 6.767383377682253E-4 0.0 16-17 0.0 0.0 0.0 7.444121715450478E-4 0.0 18-19 0.0 0.0 0.0 8.120860053218703E-4 1.3534766755364506E-4 20-21 0.0 0.0 0.0 8.797598390986928E-4 1.3534766755364506E-4 22-23 0.0 0.0 0.0 0.0011504551742059829 1.3534766755364506E-4 24-25 0.0 0.0 0.0 0.001421150509313273 1.3534766755364506E-4 26-27 0.0 0.0 0.0 0.0023009103484119657 1.3534766755364506E-4 28-29 0.0 0.0 0.0 0.003383691688841126 1.3534766755364506E-4 30-31 0.0 0.0 0.0 0.006361340375021317 2.030215013304676E-4 32-33 0.0 0.0 0.0 0.009474336728755153 4.0604300266093514E-4 34-35 0.0 0.0 0.0 0.012790354583819458 4.0604300266093514E-4 36-37 0.0 0.0 0.0 0.01854263045484937 4.737168364377577E-4 38-39 0.0 0.0 0.0 0.023482820320557415 5.413906702145802E-4 40-41 0.0 0.0 0.0 0.028761379355149573 5.413906702145802E-4 42-43 0.0 0.0 0.0 0.038641759086565655 5.413906702145802E-4 44-45 0.0 0.0 0.0 0.054680457691672596 5.413906702145802E-4 46-47 0.0 0.0 0.0 0.08831435307875339 5.413906702145802E-4 48-49 0.0 0.0 0.0 0.15422866717737854 5.413906702145802E-4 50-51 0.0 0.0 0.0 0.19388553377059653 5.413906702145802E-4 52-53 0.0 0.0 0.0 0.2288729058332138 5.413906702145802E-4 54-55 0.0 0.0 0.0 0.2778687614876333 5.413906702145802E-4 56-57 0.0 0.0 0.0 0.3209769936034692 5.413906702145802E-4 58-59 0.0 0.0 0.0 0.3709879567645411 5.413906702145802E-4 60-61 0.0 0.0 0.0 0.4488805394416638 6.767383377682253E-4 62-63 0.0 0.0 0.0 0.5084335131652676 6.767383377682253E-4 64-65 0.0 0.0 0.0 0.5771224544487426 6.767383377682253E-4 66-67 0.0 0.0 0.0 0.6804603986259505 6.767383377682253E-4 68-69 0.0 0.0 0.0 0.7935433748670209 6.767383377682253E-4 70-71 0.0 0.0 0.0 0.9054082221001085 6.767383377682253E-4 72-73 0.0 0.0 0.0 1.0314169005925522 6.767383377682253E-4 74-75 0.0 0.0 0.0 1.145176615171391 6.767383377682253E-4 76-77 0.0 0.0 0.0 1.248785254683706 6.767383377682253E-4 78-79 0.0 0.0 0.0 1.3832531623982525 6.767383377682253E-4 80-81 0.0 0.0 0.0 1.5422866717737853 6.767383377682253E-4 82-83 0.0 0.0 0.0 1.7022676148221938 6.767383377682253E-4 84-85 0.0 0.0 0.0 1.8690159412482845 6.767383377682253E-4 86-87 0.0 0.0 0.0 2.1625850321721405 6.767383377682253E-4 88-89 0.0 0.0 0.0 2.5926522458238477 6.767383377682253E-4 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TACCGTC 50 0.0016153576 38.000404 7 TATACGG 90 8.936883E-4 26.392744 5 GTACCTA 95 0.0012265474 24.998575 6 GACGCGC 50 0.0016544173 23.748646 60-61 TCGACGC 50 0.0016544173 23.748646 58-59 GTACATG 2525 0.0 23.716063 1 TACACCG 105 0.0021936214 22.62235 5 TACCTGG 585 0.0 21.926277 2 ACAGTCG 175 4.040885E-6 21.717457 5 TACATGG 2625 0.0 21.717457 2 ACGATTC 110 0.0028739672 21.594063 5 AGTACTT 3720 0.0 21.004929 12-13 ACATGGG 2505 0.0 20.86133 3 AGAGTAC 4850 0.0 20.76311 10-11 TAGGGCG 115 0.0037188884 20.655191 5 AGGACCG 255 1.1881639E-8 20.49319 5 GGTGTGC 585 0.0 20.299362 8 GAGTACT 3685 0.0 20.173214 12-13 CACTAGG 190 8.234296E-6 20.002922 2 TATAGGG 215 1.076216E-6 19.886625 2 >>END_MODULE