FastQCFastQC Report
Thu 26 May 2016
SRR937684_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937684_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences553745
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT25820.4662796052334558No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT23330.42131305926012874No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT22120.39946184615662444No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT18540.3348111495363389No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT14380.25968631770941497No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC14000.2528239532636864No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11090.20027268869244869No Hit
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT9700.17517088190412555No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT7790.1406784711374369No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN7590.1370667003765271No Hit
ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC6720.12135549756656944No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC6240.11268724774038592No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAAAGCG407.4915424E-659.445
TGGACCG702.038806E-433.9657175
GTACATG19400.028.446051
ACATGGG19650.027.589923
TGCACCG1405.741749E-727.1725735
TACATGG20300.026.70652
GTACTAA1257.2935745E-626.6411971
CATGGGG11900.023.9779724
GAGTACT20650.023.2262912-13
CCCTATA1452.3102391E-522.958222
CATGGGA8600.022.6721954
GTACCTG4750.022.0340731
CCGCTCC1100.00287689321.5890989
GTACTTT22850.020.88614814-15
TACCTGG5150.020.3153172
ATGGGGG6350.020.2189625
ATGGGAG4950.020.1735765
GGTCTAG1908.129016E-620.0309751
AGAGTAC31100.019.92639210-11
AGTACTT22900.018.66317612-13
Produced by FastQC (version 0.11.5)