##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937684_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 553745 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.895376030483344 33.0 31.0 34.0 30.0 34.0 2 32.01647509232589 33.0 31.0 34.0 30.0 34.0 3 32.06586786336671 34.0 31.0 34.0 30.0 34.0 4 35.630430974546044 37.0 35.0 37.0 35.0 37.0 5 35.619837650904294 37.0 35.0 37.0 35.0 37.0 6 35.689552050131375 37.0 35.0 37.0 35.0 37.0 7 35.61429177690092 37.0 35.0 37.0 33.0 37.0 8 35.61635048623464 37.0 35.0 37.0 33.0 37.0 9 37.186230123974035 39.0 37.0 39.0 34.0 39.0 10-11 37.12733388111857 39.0 37.0 39.0 34.0 39.0 12-13 37.04135838698318 39.0 37.0 39.0 33.5 39.0 14-15 38.209619951421686 40.0 38.0 41.0 33.5 41.0 16-17 38.11935457656503 40.0 38.0 41.0 33.0 41.0 18-19 38.031227369998824 40.0 38.0 41.0 33.0 41.0 20-21 38.04112181599834 40.0 38.0 41.0 33.5 41.0 22-23 38.03354612682733 40.0 38.0 41.0 33.5 41.0 24-25 37.95588944369701 40.0 38.0 41.0 33.0 41.0 26-27 37.83815474631825 40.0 38.0 41.0 33.0 41.0 28-29 37.68992496546244 40.0 38.0 41.0 33.0 41.0 30-31 37.461792883005714 40.0 37.0 41.0 32.0 41.0 32-33 37.34202746751664 40.0 37.0 41.0 32.5 41.0 34-35 37.33123188471228 40.0 37.0 41.0 32.0 41.0 36-37 37.18159170737434 40.0 37.0 41.0 31.5 41.0 38-39 37.0138592673523 40.0 37.0 41.0 31.0 41.0 40-41 36.80721902680837 39.5 36.0 41.0 30.5 41.0 42-43 36.546731798932726 39.0 36.0 41.0 30.0 41.0 44-45 36.28415245284382 39.0 36.0 41.0 30.0 41.0 46-47 35.90948812178891 39.0 35.0 40.5 28.5 41.0 48-49 35.55254945868586 39.0 35.0 40.0 27.0 41.0 50-51 34.69061932839123 38.0 34.0 39.5 26.0 40.5 52-53 34.80832964631735 38.0 34.5 39.5 26.0 40.5 54-55 35.064435796260014 39.0 35.0 40.0 26.0 41.0 56-57 34.77524672909011 38.0 34.5 40.0 25.5 41.0 58-59 34.48404590560637 38.0 34.0 40.0 24.0 41.0 60-61 34.08780305014041 38.0 34.0 40.0 23.0 41.0 62-63 33.7290404428031 37.5 33.0 40.0 22.0 41.0 64-65 33.270409665098555 37.0 33.0 39.5 20.5 41.0 66-67 32.67013246169266 36.0 32.0 39.0 18.0 40.5 68-69 32.21719834942076 35.5 31.5 39.0 16.5 40.0 70-71 31.70774002474063 35.0 31.0 38.0 12.0 40.0 72-73 31.221684168705814 35.0 31.0 37.5 7.5 39.5 74-75 30.654140443706037 35.0 30.0 37.0 6.0 39.0 76-77 30.105766192019793 34.0 29.5 36.0 2.0 39.0 78-79 29.558087206205023 34.0 29.0 36.0 2.0 38.5 80-81 28.948157545440594 34.0 28.5 35.5 2.0 37.0 82-83 28.368139667175324 34.0 27.5 35.0 2.0 37.0 84-85 27.795927728467074 33.0 26.0 35.0 2.0 36.0 86-87 27.218718904911107 33.0 26.0 35.0 2.0 36.0 88-89 26.636180010654723 32.5 25.0 35.0 2.0 35.5 90-91 26.017721153238405 32.0 23.5 34.5 2.0 35.0 92-93 25.299425728449016 32.0 20.0 34.0 2.0 35.0 94-95 24.503893488880262 31.0 15.5 34.0 2.0 35.0 96-97 23.593861795591835 31.0 2.0 34.0 2.0 35.0 98-99 22.387090628357818 30.5 2.0 34.0 2.0 35.0 100-101 19.228558271406513 25.5 2.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.047838044851040706 1101 2 -0.016024391555784945 1101 3 -0.1682274928751113 1101 4 0.013633772194211247 1101 5 0.03255458419754831 1101 6 -0.03590136294391044 1101 7 -0.01913178770138302 1101 8 -0.009829445059601483 1101 9 0.12835429207114402 1101 10-11 0.14883691945334476 1101 12-13 0.11523822215111323 1101 14-15 0.33597511274463443 1101 16-17 0.24942279193316352 1101 18-19 0.4004108915864535 1101 20-21 0.30574509909936154 1101 22-23 0.38287113518344995 1101 24-25 0.3413023771695478 1101 26-27 0.335472577058745 1101 28-29 0.5305437433414042 1101 30-31 0.31931112769517966 1101 32-33 0.5296363503905042 1101 34-35 0.696598397804479 1101 36-37 0.6350295876381793 1101 38-39 0.6260438981983185 1101 40-41 0.6481315027613945 1101 42-43 0.7637525069697944 1101 44-45 0.8496224107382204 1101 46-47 0.9475189337483769 1101 48-49 1.1179384665455245 1101 50-51 1.0992332745015219 1101 52-53 1.2069790306706167 1101 54-55 1.2096059820344465 1101 56-57 1.3011365389868246 1101 58-59 1.3069757392462762 1101 60-61 1.3195058680051943 1101 62-63 1.1791546844221372 1101 64-65 1.318212704466717 1101 66-67 1.3921427796867718 1101 68-69 1.2512668520585066 1101 70-71 1.3810539208648684 1101 72-73 1.5914862490236068 1101 74-75 1.748371522145078 1101 76-77 1.6771401311183851 1101 78-79 1.7451530678405724 1101 80-81 2.0034699176799435 1101 82-83 2.1167544586447704 1101 84-85 2.3329690666586096 1101 86-87 2.505106980676935 1101 88-89 2.3405343701268855 1101 90-91 2.767488844754652 1101 92-93 2.75218281842157 1101 94-95 2.889406264021847 1101 96-97 3.0509708999362637 1101 98-99 3.256353762403574 1101 100-101 3.6699134596422027 1102 1 0.19655915425011372 1102 2 0.2593428400826312 1102 3 0.15303085329848543 1102 4 0.25326910860921004 1102 5 0.13033578604655105 1102 6 0.13412431759024201 1102 7 0.1307398096278405 1102 8 0.1396415359368035 1102 9 0.33342876562019796 1102 10-11 0.3654779055858626 1102 12-13 0.18123462687633207 1102 14-15 0.4712704388874158 1102 16-17 0.5804346049788762 1102 18-19 0.47864663888999104 1102 20-21 0.547904832021807 1102 22-23 0.41893430929746955 1102 24-25 0.4006629318690855 1102 26-27 0.504185982297571 1102 28-29 0.472947441338313 1102 30-31 0.5394652109001115 1102 32-33 0.5384807263535194 1102 34-35 0.6425408734079738 1102 36-37 0.5792951243613444 1102 38-39 0.6146468771402809 1102 40-41 0.8497878972143909 1102 42-43 0.9558223580227008 1102 44-45 1.332298322397179 1102 46-47 1.242251856193164 1102 48-49 1.3270011270488595 1102 50-51 1.5107766746042444 1102 52-53 1.339174716340878 1102 54-55 1.2420302244586878 1102 56-57 1.3381447567577567 1102 58-59 1.395727665286337 1102 60-61 1.2632090010303614 1102 62-63 1.2012836007035972 1102 64-65 1.4396277018986652 1102 66-67 1.6071504838470148 1102 68-69 1.5831697796394053 1102 70-71 1.7096440595397517 1102 72-73 1.5090928232403549 1102 74-75 1.5960063449493944 1102 76-77 1.5725510196648713 1102 78-79 1.7787560467825365 1102 80-81 1.84946632240516 1102 82-83 1.9948515310638726 1102 84-85 2.201980366093636 1102 86-87 2.238743344313299 1102 88-89 2.3290140825049015 1102 90-91 2.400909491904116 1102 92-93 2.5908191820579347 1102 94-95 2.4267996898051045 1102 96-97 2.569255440254704 1102 98-99 2.751469324807271 1102 100-101 3.152234979929826 1103 1 0.1874943089849168 1103 2 -0.009457227376678645 1103 3 -0.12176887686425886 1103 4 -0.15056342364477615 1103 5 0.03067308261455537 1103 6 -0.043942973075076 1103 7 0.08177278036736624 1103 8 -0.04381406740243676 1103 9 -0.11545393949828053 1103 10-11 -0.07162893310206186 1103 12-13 0.07254713214658892 1103 14-15 0.2807105265845209 1103 16-17 0.3246331040091448 1103 18-19 0.29154612451272754 1103 20-21 0.24237106020293453 1103 22-23 0.08174947077819894 1103 24-25 0.0022250365996683286 1103 26-27 0.18354132423196035 1103 28-29 -0.033553950010023925 1103 30-31 0.12703161616193626 1103 32-33 0.20971776151669275 1103 34-35 0.02990911693608922 1103 36-37 0.1784624234590808 1103 38-39 0.38076122067683826 1103 40-41 0.5633282463163383 1103 42-43 0.4318574368657693 1103 44-45 0.5570896201457245 1103 46-47 0.2683059079681911 1103 48-49 0.7146413159349407 1103 50-51 0.8490071324843385 1103 52-53 0.777481065948777 1103 54-55 0.7074531100308334 1103 56-57 0.6132079998642581 1103 58-59 0.7717880413720124 1103 60-61 1.0526221050020297 1103 62-63 1.3091320702204214 1103 64-65 0.9671995882297182 1103 66-67 1.1059193513737924 1103 68-69 1.1806969741751985 1103 70-71 0.9922796105980147 1103 72-73 0.8041682933474412 1103 74-75 0.8531928699514992 1103 76-77 1.053449493397899 1103 78-79 1.1611639226573978 1103 80-81 1.2636643998147257 1103 82-83 1.1965780678713642 1103 84-85 1.1576954348178106 1103 86-87 1.3205841403331995 1103 88-89 1.5951748043195586 1103 90-91 1.9008040867266125 1103 92-93 2.011590326336542 1103 94-95 2.5214008366134344 1103 96-97 2.728628068638205 1103 98-99 2.912355571539713 1103 100-101 3.0448524012975646 1104 1 0.07891108528578172 1104 2 -0.03128275021839855 1104 3 -0.07124490099890224 1104 4 -0.017183212239793022 1104 5 -0.04870359025211002 1104 6 0.28524996799465185 1104 7 0.06609433369494866 1104 8 0.1310124081226718 1104 9 0.18147025412327622 1104 10-11 0.18826309611356606 1104 12-13 0.07988959914291627 1104 14-15 0.1405730679808883 1104 16-17 0.13833133020340682 1104 18-19 0.18017970433398744 1104 20-21 -0.011500917295592217 1104 22-23 0.030728738572967984 1104 24-25 0.06612967752876386 1104 26-27 0.04659535499003198 1104 28-29 0.18168217937346043 1104 30-31 0.07919783673082037 1104 32-33 0.15272007517635444 1104 34-35 0.16869013585365877 1104 36-37 0.25135177608802906 1104 38-39 0.21970328845345222 1104 40-41 0.20567502307086727 1104 42-43 0.19283144221894588 1104 44-45 0.612113890889276 1104 46-47 0.5828297931017872 1104 48-49 0.6666646339950901 1104 50-51 0.38590546755669664 1104 52-53 0.49863971041639843 1104 54-55 0.7645732842357944 1104 56-57 0.8228119557686213 1104 58-59 0.8764516534030378 1104 60-61 0.8707253577353242 1104 62-63 0.9941136970407243 1104 64-65 1.0003643117247094 1104 66-67 1.257756669343216 1104 68-69 0.8833281949709146 1104 70-71 1.0687982332900283 1104 72-73 1.0666847384774165 1104 74-75 1.3463562324702139 1104 76-77 1.3470710512620734 1104 78-79 1.5113453861605493 1104 80-81 1.4150412541448993 1104 82-83 1.516881565580384 1104 84-85 1.9012466755745159 1104 86-87 1.895025005914107 1104 88-89 2.1958507558688005 1104 90-91 1.9557194793682697 1104 92-93 2.2548262742565193 1104 94-95 2.543346579025993 1104 96-97 2.7269495312340446 1104 98-99 2.9583837891144533 1104 100-101 3.5026462587579807 1105 1 0.24689609523439415 1105 2 0.25056195382574487 1105 3 0.0967889364786636 1105 4 0.1813373136326888 1105 5 0.10906425926144436 1105 6 0.08000955344893157 1105 7 0.1583417427841951 1105 8 0.025490014597203015 1105 9 0.10420679298388791 1105 10-11 0.0029260030605087195 1105 12-13 0.07706370590430822 1105 14-15 0.10095868339085001 1105 16-17 0.1732124962047905 1105 18-19 0.06229479081974887 1105 20-21 0.039523850468469846 1105 22-23 0.27145455979096766 1105 24-25 0.29882337023270367 1105 26-27 0.25298626818689485 1105 28-29 0.37567700365538315 1105 30-31 -0.057311727361415876 1105 32-33 0.1955326629133225 1105 34-35 0.5420711980965578 1105 36-37 0.3503709531000254 1105 38-39 0.37877117092558876 1105 40-41 0.4562731603006398 1105 42-43 0.684533814016163 1105 44-45 0.6895238346155423 1105 46-47 0.7011297406121955 1105 48-49 0.9673506607769866 1105 50-51 0.7760496308359492 1105 52-53 0.94077603687726 1105 54-55 0.9518695818884098 1105 56-57 0.8759485141602497 1105 58-59 0.7144602226343721 1105 60-61 0.9136752728974926 1105 62-63 1.1453905369230526 1105 64-65 1.0537877318489777 1105 66-67 1.3680244883395645 1105 68-69 1.1972617407040076 1105 70-71 1.328636980375638 1105 72-73 1.1007816122948775 1105 74-75 1.1638114637295942 1105 76-77 1.1710722231227706 1105 78-79 1.1457919871541904 1105 80-81 1.347705039987357 1105 82-83 1.1508398912844164 1105 84-85 1.6624068176626245 1105 86-87 1.6483818985301646 1105 88-89 1.7585117341283443 1105 90-91 2.0552727074782773 1105 92-93 2.335297093704522 1105 94-95 2.296456282276683 1105 96-97 2.3025955804766056 1105 98-99 2.005944854042859 1105 100-101 2.8194972493464725 1106 1 -0.11813173944727495 1106 2 -0.12945378636846527 1106 3 -0.22045699248213424 1106 4 -0.12106538071819983 1106 5 -0.42975962416853264 1106 6 -0.09613016392199114 1106 7 -0.2674594446745715 1106 8 0.06415186661623551 1106 9 -0.14679722308036958 1106 10-11 -0.4326057493092037 1106 12-13 -0.6668803701115706 1106 14-15 -0.7208443790028127 1106 16-17 -0.8256937917713145 1106 18-19 -0.2912230268486198 1106 20-21 -0.19409072309957764 1106 22-23 -0.028995077374403877 1106 24-25 -0.7486373660843171 1106 26-27 -0.6806936966291417 1106 28-29 -0.18521208584382265 1106 30-31 -0.019295107568119363 1106 32-33 -0.11879959406849849 1106 34-35 0.005217732360414118 1106 36-37 -0.2198407293647051 1106 38-39 -0.3023047176430893 1106 40-41 -0.33916218337956394 1106 42-43 -0.3540817433140262 1106 44-45 -0.4485471814371351 1106 46-47 -0.43329846211225487 1106 48-49 -0.4948150047670268 1106 50-51 -0.6582813530419216 1106 52-53 -0.690883164770824 1106 54-55 -0.3316969609399649 1106 56-57 -0.6106231326575724 1106 58-59 -1.0044905082430304 1106 60-61 -0.5087955903827179 1106 62-63 -0.7851558561420049 1106 64-65 -0.5920868326916064 1106 66-67 -0.4067865714615948 1106 68-69 -0.7013818249129358 1106 70-71 -0.5850957603856806 1106 72-73 -0.705156838916313 1106 74-75 -0.6496932756891738 1106 76-77 0.08600573237592002 1106 78-79 0.3126930206831169 1106 80-81 0.31739359246817145 1106 82-83 0.4415068820139041 1106 84-85 0.5164657045068353 1106 86-87 0.8537769658310701 1106 88-89 0.9395702621887132 1106 90-91 0.9465264833888369 1106 92-93 1.084214955352845 1106 94-95 0.9034172674276633 1106 96-97 0.6122144594507155 1106 98-99 0.9547573952740684 1106 100-101 1.2696986312429352 1107 1 0.05167341346252741 1107 2 0.22934874277257222 1107 3 0.3259923171655963 1107 4 0.39570523307163796 1107 5 0.31127009753993207 1107 6 0.26202717617865545 1107 7 0.19669815350169273 1107 8 0.19349936543338941 1107 9 0.3039490455763456 1107 10-11 0.3709083803307749 1107 12-13 0.4434426545346497 1107 14-15 0.5680239594203442 1107 16-17 0.5686629547554176 1107 18-19 0.4840834379455643 1107 20-21 0.6572964333372653 1107 22-23 0.5429435006289509 1107 24-25 0.67983697689818 1107 26-27 0.39995923467972716 1107 28-29 0.3933117216317683 1107 30-31 0.6650913176544648 1107 32-33 0.842553582412215 1107 34-35 0.7903568781301189 1107 36-37 0.9269698861456419 1107 38-39 0.9897164445574305 1107 40-41 1.1260148134805164 1107 42-43 1.100514153283683 1107 44-45 0.7175373813397528 1107 46-47 0.7892014303562505 1107 48-49 0.9482234054871768 1107 50-51 0.4094548936783653 1107 52-53 0.7521079316204151 1107 54-55 0.8701396771950058 1107 56-57 0.6064915819538115 1107 58-59 0.45835520555111486 1107 60-61 0.7123235975393385 1107 62-63 1.2628634135040002 1107 64-65 1.3410801852446497 1107 66-67 1.3805462170454348 1107 68-69 1.727711899548332 1107 70-71 1.3116825956726466 1107 72-73 1.417601129168336 1107 74-75 1.0681182430858271 1107 76-77 1.0071808366814778 1107 78-79 1.2417609375827716 1107 80-81 1.7539629072774083 1107 82-83 1.8555332917519607 1107 84-85 1.734561106459605 1107 86-87 1.6429722000346878 1107 88-89 1.2959210729762738 1107 90-91 1.8709533404579588 1107 92-93 2.166067938276843 1107 94-95 2.3467739709417934 1107 96-97 2.2226579193844778 1107 98-99 1.7884749745247852 1107 100-101 2.303807625178159 1108 1 -0.11487479786180188 1108 2 0.11032481479342238 1108 3 0.16418231953469942 1108 4 0.19527879383922908 1108 5 0.17760508924805407 1108 6 0.10372345668090333 1108 7 0.2732203046507067 1108 8 0.03097863574847537 1108 9 0.18653611025296613 1108 10-11 0.2879422585586866 1108 12-13 0.29414154105442947 1108 14-15 0.07880339557291904 1108 16-17 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1004.0 10 1205.0 11 1529.0 12 1949.0 13 2518.0 14 2728.0 15 3413.0 16 4432.0 17 5804.0 18 5293.0 19 3749.0 20 3827.0 21 4142.0 22 4580.0 23 4923.0 24 5591.0 25 6479.0 26 7390.0 27 8757.0 28 10256.0 29 12264.0 30 14902.0 31 18708.0 32 23674.0 33 30538.0 34 41591.0 35 57969.0 36 82062.0 37 104419.0 38 66353.0 39 5671.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.33342088816833 18.318403724401534 14.579510357133152 22.76866503029699 2 15.07467902233762 19.637441449075407 41.205728077465565 24.08215145112141 3 19.950530636782247 23.628501741024625 30.050103121531308 26.370864500661824 4 12.435584977131082 15.865560460492723 37.90265047675265 33.79620408562354 5 13.394595083970847 37.09148522022543 34.7841202299579 14.729799465845819 6 31.721440967352578 35.597293526432274 17.703008137878868 14.978257368336287 7 28.180416624444476 32.35715629930949 21.047098249829904 18.415328826416133 8 25.71496301229063 34.52596377955036 19.87691272058717 19.88216048757184 9 25.428216213672876 17.417292967753262 21.325484135414953 35.82900668315891 10-11 24.98769426770035 26.067250827017595 27.510459872454707 21.43459503282735 12-13 25.937280010423773 23.53402113347678 28.212051402232785 22.316647453866658 14-15 22.63488202912084 24.954668346018956 27.045081922713603 25.365367702146603 16-17 21.70751920787206 28.460945501395123 27.586412805489307 22.245122485243503 18-19 21.464092921174597 27.388188719221763 29.65558045427446 21.49213790532918 20-21 22.211235125577087 25.924944157302253 29.75029334944908 22.11352736767158 22-23 21.771377958561814 25.77541664819521 30.062453123968048 22.390752269274927 24-25 22.224061840112476 25.910565619170146 29.913724936714132 21.95164760400325 26-27 21.770404410267822 25.886346753960005 30.470332661080548 21.87291617469163 28-29 21.389391581309493 26.9145644069637 30.00959137138514 21.68645264034167 30-31 21.579267398869664 26.037091528994043 29.873737510835557 22.50990356130074 32-33 21.539055933014655 26.173433333604777 30.161138692920808 22.126372040459756 34-35 22.380476699765286 26.012548159488663 29.68597027457921 21.92100486616684 36-37 21.901551361773006 25.751501646178777 30.37337813617639 21.97356885587182 38-39 21.615409040291144 26.486368640591117 30.174258138695475 21.72396418042226 40-41 21.75042245774518 26.57765804623696 29.894810735311676 21.77710876070618 42-43 21.383524899015256 26.492154175317655 29.396534176493738 22.72778674917335 44-45 21.809230078863965 26.22604655118287 29.381329043780042 22.58339432617312 46-47 22.256321821401137 26.68505752950214 29.19596221338166 21.86265843571507 48-49 22.046765317442077 26.568751820973635 29.109428526445512 22.275054335138776 50-51 21.349715454142427 26.92319523736282 29.601726275027822 22.125363033466936 52-53 21.989720666346948 27.204491779247693 28.5393573606725 22.266430193732862 54-55 21.640460234351462 26.594213322982856 28.979166044642767 22.786160398022915 56-57 21.949278030098135 26.84210193058994 28.706297596509145 22.50232244280278 58-59 22.546877317637016 26.608372254561107 28.694109900764435 22.150640527037442 60-61 22.02421927790839 26.748127289157768 29.502760331909222 21.72489310102462 62-63 21.60871154013141 26.98884193464355 29.44477408897508 21.957672436249965 64-65 21.536328085777324 27.378383977088415 28.88136868852711 22.20391924860715 66-67 21.69371230699508 27.046605221094605 28.40647069093251 22.8532117809778 68-69 21.46024423337856 26.83600180913614 29.047670737222976 22.656083220262325 70-71 22.127691524169553 26.67140677056902 28.649133709827844 22.55176799543358 72-73 21.89745297032838 26.42862183204477 29.28414660845367 22.38977858917318 74-75 21.290039491004826 27.532603827868073 28.643508863989 22.5338478171381 76-77 21.68383225731739 26.949223386699867 28.48498885382589 22.881955502156856 78-79 21.8321435259612 26.784552738878975 28.695693081264857 22.68761065389497 80-81 22.044305005595348 26.92369974931766 28.449686669688244 22.582308575398756 82-83 21.8164960017368 26.785740130983825 29.027752650432394 22.37001121684698 84-85 21.71289893364425 27.077693183699203 28.58718039403333 22.622227488623217 86-87 21.603559042069136 27.18012132440075 28.727432594037268 22.488887039492848 88-89 21.6238367533035 26.896859923703136 28.188134996774977 23.291168326218383 90-91 21.720880667683463 26.591727773887904 28.507445944307847 23.17994561412079 92-93 22.10888907382414 26.54917448520659 28.738425967804382 22.60351047316489 94-95 21.773729574040036 26.358263015443402 28.85750314802217 23.01050426249439 96-97 21.14377834409425 26.56304703593034 29.401142946057902 22.892031673917508 98-99 21.530584089850237 26.97524414349622 28.314584379339514 23.179587387314037 100-101 21.62217410259611 25.962075945794872 29.1045950624922 23.311154889116825 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 695.0 1 403.0 2 124.0 3 146.0 4 123.0 5 108.0 6 178.5 7 322.0 8 528.0 9 694.5 10 689.5 11 621.5 12 623.5 13 659.0 14 726.5 15 792.0 16 831.0 17 852.5 18 906.0 19 987.5 20 1109.0 21 1281.0 22 1496.5 23 1805.0 24 2176.5 25 2681.0 26 3352.0 27 4194.0 28 4969.0 29 5670.0 30 6518.0 31 7371.0 32 8373.5 33 9504.5 34 10613.5 35 11608.5 36 12702.0 37 13897.0 38 14921.0 39 15689.5 40 16265.5 41 17066.5 42 17855.5 43 18588.5 44 19494.0 45 20218.0 46 20779.5 47 23061.5 48 27301.0 49 26929.5 50 22846.5 51 20305.5 52 18806.0 53 18119.5 54 17534.0 55 16150.0 56 14529.5 57 13060.5 58 11401.0 59 9927.0 60 8206.0 61 6512.5 62 4959.0 63 3604.5 64 2625.0 65 1903.0 66 1402.0 67 1042.0 68 764.0 69 549.5 70 407.5 71 266.5 72 183.0 73 143.0 74 116.5 75 74.0 76 42.0 77 29.0 78 23.0 79 16.0 80 7.0 81 6.0 82 7.0 83 3.5 84 2.0 85 3.5 86 4.0 87 3.0 88 1.5 89 0.5 90 0.0 91 1.0 92 1.0 93 0.5 94 0.5 95 0.5 96 0.5 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.30880640005778837 2 0.26149220308986987 3 0.26799339045950754 4 0.26492338531273424 5 0.2654651509268707 6 0.20659328752404083 7 0.20099504284463066 8 0.20388445945335848 9 0.20984388120885966 10-11 0.2089409385186322 12-13 0.20984388120885966 14-15 0.20785740729035931 16-17 0.1999115116163577 18-19 0.1916947331352879 20-21 0.19440356120597027 22-23 0.1969318007386071 24-25 0.19756386062176634 26-27 0.20325239957019928 28-29 0.21038564682299615 30-31 0.21056623536104163 32-33 0.20704475886915455 34-35 0.20839917290449575 36-37 0.19891827465710754 38-39 0.1860964884558777 40-41 0.1854644285727185 42-43 0.1918753216733334 44-45 0.19584826951033418 46-47 0.20144651418974438 48-49 0.20876034998058673 50-51 0.20045327723049416 52-53 0.2130944748936785 54-55 0.20108533711365337 56-57 0.17815059278187614 58-59 0.16596086646380553 60-61 0.18122059792864947 62-63 0.17354558506171613 64-65 0.18266530623301339 66-67 0.18176236354278594 68-69 0.17968559535526282 70-71 0.18320707184714985 72-73 0.19178502740431067 74-75 0.19639003512447067 76-77 0.19666091793153886 78-79 0.19223649874942436 80-81 0.19025002483092399 82-83 0.18149148073571772 84-85 0.17598353032533023 86-87 0.18275560050203615 88-89 0.18699943114610518 90-91 0.18564501711076398 92-93 0.18040794950744476 94-95 0.18365854319226357 96-97 0.18970825921678752 98-99 0.18916649360265103 100-101 0.17264264237148869 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 553745.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 73.30313641672363 #Duplication Level Percentage of deduplicated Percentage of total 1 88.06380433468544 64.55353062521105 2 7.463826272908516 10.942437509474779 3 1.9161579738364318 4.213811680563741 4 0.8244545484367979 2.417404169338035 5 0.4419027139819452 1.6196427462969465 6 0.2853819732060911 1.255163622767992 7 0.18016021555433867 0.9244416200352218 8 0.1449507364243021 0.8500274884652134 9 0.11224832123495694 0.7405338603630838 >10 0.5326822255034909 6.7866727663722894 >50 0.022540957907182833 1.1520864684380336 >100 0.008174186845311111 1.2221732343730332 >500 0.0019816210534056625 0.9746881476911545 >1k 0.0017339184217299549 2.3473860606093977 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 2582 0.4662796052334558 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 2333 0.42131305926012874 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2212 0.39946184615662444 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1854 0.3348111495363389 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 1438 0.25968631770941497 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 1400 0.2528239532636864 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1109 0.20027268869244869 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 970 0.17517088190412555 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 779 0.1406784711374369 No Hit NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 759 0.1370667003765271 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 672 0.12135549756656944 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 624 0.11268724774038592 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 3.611770760909805E-4 0.0 24-25 0.0 0.0 0.0 8.126484212047061E-4 0.0 26-27 0.0 0.0 0.0 0.001535002573386667 0.0 28-29 0.0 0.0 0.0 0.0030700051467733346 0.0 30-31 0.0 0.0 0.0 0.006501187369637649 9.029426902274512E-5 32-33 0.0 0.0 0.0 0.011467372165888631 1.8058853804549024E-4 34-35 0.0 0.0 0.0 0.015620908540934907 1.8058853804549024E-4 36-37 0.0 0.0 0.0 0.021580330296436086 1.8058853804549024E-4 38-39 0.0 0.0 0.0 0.027359163513891775 1.8058853804549024E-4 40-41 0.0 0.0 0.0 0.03467299930473413 1.8058853804549024E-4 42-43 0.0 0.0 0.0 0.04505684024234982 1.8058853804549024E-4 44-45 0.0 0.0 0.0 0.06465069662028551 1.8058853804549024E-4 46-47 0.0 0.0 0.0 0.10501223487345257 1.8058853804549024E-4 48-49 0.0 0.0 0.0 0.18239442342594514 1.8058853804549024E-4 50-51 0.0 0.0 0.0 0.2277221464753632 1.8058853804549024E-4 52-53 0.0 0.0 0.0 0.27142457268237186 1.8058853804549024E-4 54-55 0.0 0.0 0.0 0.32876143351181497 1.8058853804549024E-4 56-57 0.0 0.0 0.0 0.3767979846319154 1.8058853804549024E-4 58-59 0.0 0.0 0.0 0.4257374784422433 1.8058853804549024E-4 60-61 0.0 0.0 0.0 0.5135035079323516 1.8058853804549024E-4 62-63 0.0 0.0 0.0 0.5783347930906826 1.8058853804549024E-4 64-65 0.0 0.0 0.0 0.6558072759121978 1.8058853804549024E-4 66-67 0.0 0.0 0.0 0.7730092371037209 1.8058853804549024E-4 68-69 0.0 0.0 0.0 0.8946356174773586 1.8058853804549024E-4 70-71 0.0 0.0 0.0 1.0093093391362449 1.8058853804549024E-4 72-73 0.0 0.0 0.0 1.155676349222115 3.611770760909805E-4 74-75 0.0 0.0 0.0 1.2953615834003016 3.611770760909805E-4 76-77 0.0 9.029426902274512E-5 0.0 1.4010058781569135 3.611770760909805E-4 78-79 0.0 1.8058853804549024E-4 0.0 1.5472825939737604 3.611770760909805E-4 80-81 0.0 1.8058853804549024E-4 0.0 1.7100831610217702 3.611770760909805E-4 82-83 0.0 1.8058853804549024E-4 0.0 1.8842608059666452 3.611770760909805E-4 84-85 0.0 1.8058853804549024E-4 0.0 2.0499507896233826 3.611770760909805E-4 86-87 0.0 1.8058853804549024E-4 0.0 2.359479543833353 3.611770760909805E-4 88-89 0.0 1.8058853804549024E-4 0.0 2.845172416906699 3.611770760909805E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TAAAGCG 40 7.4915424E-6 59.44 5 TGGACCG 70 2.038806E-4 33.965717 5 GTACATG 1940 0.0 28.44605 1 ACATGGG 1965 0.0 27.58992 3 TGCACCG 140 5.741749E-7 27.172573 5 TACATGG 2030 0.0 26.7065 2 GTACTAA 125 7.2935745E-6 26.641197 1 CATGGGG 1190 0.0 23.977972 4 GAGTACT 2065 0.0 23.22629 12-13 CCCTATA 145 2.3102391E-5 22.95822 2 CATGGGA 860 0.0 22.672195 4 GTACCTG 475 0.0 22.034073 1 CCGCTCC 110 0.002876893 21.589098 9 GTACTTT 2285 0.0 20.886148 14-15 TACCTGG 515 0.0 20.315317 2 ATGGGGG 635 0.0 20.218962 5 ATGGGAG 495 0.0 20.173576 5 GGTCTAG 190 8.129016E-6 20.030975 1 AGAGTAC 3110 0.0 19.926392 10-11 AGTACTT 2290 0.0 18.663176 12-13 >>END_MODULE